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<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GWK6</accession>
    <name>A0A1A0GWK6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_48180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000019">
        <property type="protein sequence ID" value="OBA16113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709229.1">
        <property type="nucleotide sequence ID" value="XM_018858275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GWK6"/>
    <dbReference type="STRING" id="869754.A0A1A0GWK6"/>
    <dbReference type="GeneID" id="30031251"/>
    <dbReference type="OrthoDB" id="5414461at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA16113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="66" mass="6962" checksum="61FFFD8726CB92A9" modified="2016-10-05" version="1">MPKSSLHGSGRGLLVSNSQTQASGVASSMDSDLEAFSHNPAVGRFGPMASQTGPNTNYLNERFLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYV0</accession>
    <name>A0A1A0GYV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_14062</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Membrane-associated protein that warps the membrane surface to access and bind aromatic isoprenes with high specificity, including ubiquinone (CoQ) isoprene intermediates and presents them directly to Coq7, therefore facilitating the Coq7-mediated hydroxylase step. Participates in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the COQ9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000012">
        <property type="protein sequence ID" value="OBA16949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709251.1">
        <property type="nucleotide sequence ID" value="XM_018854476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYV0"/>
    <dbReference type="STRING" id="869754.A0A1A0GYV0"/>
    <dbReference type="GeneID" id="30027452"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.10">
        <property type="entry name" value="Tetracycline Repressor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013718">
        <property type="entry name" value="COQ9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012762">
        <property type="entry name" value="Ubiq_biosynth_COQ9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02396">
        <property type="entry name" value="diverge_rpsU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21427:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS PROTEIN COQ9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08511">
        <property type="entry name" value="COQ9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="COQ9 C-terminal" evidence="9">
        <location>
            <begin position="149"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA16949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="247" mass="27697" checksum="847DADB65D1AE947" modified="2016-10-05" version="1">MFSRTPGALRLCARAYHSISHPASNVIVHSKSPEAVLFSKAIEHVPRHGFSKTAIDAAVADLQFLDLIQSAISASLGRSMEFLFVLFWLKMQRQKLQDHVLDAGLPFHQIGDEYDRAAYLLNKRLLYNRPVIGQLLGALAQLVVPYNVALSLEELHNLSDDVAFYAGDMSNDTAWYAKRMLLSSIYVKSELFLLQDNSRDFERTRRFVDSSVASVRSMGSAYTSVEQWAVFNAISLVNLIKSQLARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ04</accession>
    <name>A0A1A0GZ04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Protein URE2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_80171</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an important role in the cellular response to the nitrogen source. URE2 gene plays a major part in the repression of GLN1 and GDH2 genes by glutamine, and is required for the inactivation of glutamine synthetase. URE2 gene product may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709301.1">
        <property type="nucleotide sequence ID" value="XM_018859185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ04"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ04"/>
    <dbReference type="GeneID" id="30032161"/>
    <dbReference type="OrthoDB" id="422574at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03048">
        <property type="entry name" value="GST_N_Ure2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000034">
        <property type="entry name" value="Transcriptional regulator URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017298">
        <property type="entry name" value="Ure2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF3">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037861">
        <property type="entry name" value="Prion_URE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="8">
        <location>
            <begin position="79"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="9">
        <location>
            <begin position="172"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00058785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR037861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA17004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="321" mass="36089" checksum="71421E6430B9F2C1" modified="2016-10-05" version="1">MDGIQRSDERAADSDSINQANGGAPISNLSAGLKSVSLSDQQQNAVNLNLLQQHLHNETSESPLLQSRISCFFANQPTEGYTLFSHRSAPNGFKVAIILLELDSPFNTIFLDFNSGEQRAPEFVSINPNARVPALIDHYNENTSIWESGAIVLYLVSKYVKERGDCPLWSNNLVEQSQISSWLFFQTSGHAPMIGQALHFRYFHSCSVPSAVDRYIEEVRRVYGVIEMALAERREALIMDLDRDNAAAYSAGTTPLSSSRYFDYPVWLVGDRATVADLSFVPWNNVVDRIGINLKVEFPEVYKWTKYMMRRPAVVRALRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ68</accession>
    <name>A0A1A0GZ68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcriptional protein SWT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_48001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SWT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA16992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709289.1">
        <property type="nucleotide sequence ID" value="XM_018858248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ68"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ68"/>
    <dbReference type="GeneID" id="30031224"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18727">
        <property type="entry name" value="PIN_Swt1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000045">
        <property type="entry name" value="Transcriptional protein swt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049014">
        <property type="entry name" value="SWT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052626">
        <property type="entry name" value="SWT1_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16161:SF0">
        <property type="entry name" value="TRANSCRIPTIONAL PROTEIN SWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21693">
        <property type="entry name" value="SWT1_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="PIN" evidence="7">
        <location>
            <begin position="102"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00060839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA16992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="47812" checksum="DA2DF0901AF17A5E" modified="2016-10-05" version="1">MELPSIHAPNGLSESDRKRAITVGPSRRSQIRTEPSSSFNAGSYAPAPDIEMMAIDDMEESRMISDIVASSRSETDFSASDEMELDRHILQTSTVALQGNVSYLIIDTNFILSQLLILDELKTIGERYGIVIVIPIQVVRELDGLKGSENRASNEQGGLLDIGKLARRANDWIFSCLAQRCPTVKGQGLKEKIDRYATKDDAILDCCLYYKEEYKHTLQILFSNDKNLCLKALMNDVLTISFHKDMSAQLIAEKIYQENMMRFGYIDPNSVASVEVEVPSPVHAQSPEQITESIYAEILKVACHIVGLYMRAEYGDDISLIRDYDANSTVSLEDAVMVIIRFWRPVFSQSLRGGEPFEEHHGRSTPIMVDPPRSAHDLLQFVDFWESLLLKLYEKNSRLDGLEVIQIHAKKWKDLAHSLVAAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H222</accession>
    <name>A0A1A0H222_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-1,3-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_80081</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709367.1">
        <property type="nucleotide sequence ID" value="XM_018859169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H222"/>
    <dbReference type="GeneID" id="30032145"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508801" description="Alpha-1,3-mannosyltransferase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA17972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="641" mass="73021" checksum="FD05293B2F2FB742" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H261</accession>
    <name>A0A1A0H261_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase II transcription factor B subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">RNA polymerase II transcription factor B 38 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">RNA polymerase II transcription factor B p38 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_13945</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709435.1">
        <property type="nucleotide sequence ID" value="XM_018854457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H261"/>
    <dbReference type="STRING" id="869754.A0A1A0H261"/>
    <dbReference type="GeneID" id="30027433"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16573">
        <property type="entry name" value="RING-HC_TFB3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000037">
        <property type="entry name" value="Cdk-activating kinase assembly factor MAT1, centre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004575">
        <property type="entry name" value="MAT1/Tfb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00570">
        <property type="entry name" value="cdk7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17121">
        <property type="entry name" value="zf-C3HC4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="10"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="154"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="344"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA18040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="498" mass="57506" checksum="534ADE16DCEF0026" modified="2016-10-05" version="1">MDDDMTRDMCPICKTDRYLSPNMVFLINPECYHKICESCVDRIFSLGPAQCPYPKCSKILRKNRFKKQVFDDILIEREIDIRKRIQRIYNKTEEDFASLPEYNAYLEAVEEITFKLCEGIDTDETEKEVSKYEAEHKIEILEIAMRESQKDSDVSKFQEATERLRQEKLNIQKQMEAEDAEFQKQQKVELMEKLQSSAANAEEIMKQQKNAQLKRLLLRKRQLQQISSQLDQKFLSNKAGSRHGAGLDSADAHTPFTPFCGDRDVDKKYTLLGKPTRDVVDITDTYYDPYVNKMAQNREFLGGGWRLKTLFERALDEAFMGLSCFIETERAPHLRRGPSDTALMEKLQSSAANAEEIMKQQKNAQLKRLLLRKRQLQQISSQLDQKFLSNKAGSRHGAGLDSADAHTPFTPFCGDRDVDKKYTLLGKPTRDVVDITDTYYDPYVNKMAQNREFLGGGWRLKTLFERALDEAFMGLSCFIETERAPHLRRGPSDTAVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4S0</accession>
    <name>A0A1A0H4S0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33684</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709609.1">
        <property type="nucleotide sequence ID" value="XM_018856378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4S0"/>
    <dbReference type="STRING" id="869754.A0A1A0H4S0"/>
    <dbReference type="GeneID" id="30029354"/>
    <dbReference type="OrthoDB" id="346839at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051229">
        <property type="entry name" value="ALYREF_mRNA_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965">
        <property type="entry name" value="RNA AND EXPORT FACTOR BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19965:SF35">
        <property type="entry name" value="RNA ANNEALING PROTEIN YRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15038" checksum="F016798A59090384" modified="2016-10-05" version="1">MNYNEKGIFKGVATIAFKSSKNAVKAIEKYNNAPIDGGASKLKLELVIDPSKKPLTARITANVAPTKEQPDQKKKQLQEKKKLLQQKRKELVQAKAVKTAKSAKQEKKTKQKQAKPEPKTAEQLDQEMADYFNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4T3</accession>
    <name>A0A1A0H4T3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide:N-glycanase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33664</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglutaminase-like superfamily. PNGase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709581.1">
        <property type="nucleotide sequence ID" value="XM_018856370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4T3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4T3"/>
    <dbReference type="GeneID" id="30029346"/>
    <dbReference type="OrthoDB" id="409136at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120125">
        <property type="term" value="C:PNGase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097466">
        <property type="term" value="P:ubiquitin-dependent glycoprotein ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.620.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050883">
        <property type="entry name" value="PNGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143">
        <property type="entry name" value="PEPTIDE N-GLYCANASE PNGASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12143:SF19">
        <property type="entry name" value="PEPTIDE-N(4)-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="6">
        <location>
            <begin position="171"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="360" mass="41442" checksum="57778FCC0797F8C9" modified="2016-10-05" version="1">MGVCLDYKHLANLLLISYTKGMLDLAKTKGSRRIYVKSQADSRIIRSIQRISHDLKHYDISESLEKALDLIDLDKIYAGVYQREMSSVNTALGYEDLVVLETLRYFKADFFSWVNRPACPKCKKDGDNIQPKGSEAPPEINPDEISVIEVYTCIDCNQRVEFPRINNPARLLETRRGRCGEWVNCFMLILKAILGPEVPTRYIWNAEDHVWCEYYSHKMKRWVHLDPCEDVFDEPSLYSRNWGKKMSWVLGISHDYVVDLSGKYVTERGKTIPKNTVANEQAIARFLESYNALLLSQNWDALQLLDASVDEKYLKLYYETLLPQAKERNDSKVAHSESENLPQGRQTGDALWTAARGENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H567</accession>
    <name>A0A1A0H567_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_224779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709587.1">
        <property type="nucleotide sequence ID" value="XM_018855166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H567"/>
    <dbReference type="STRING" id="869754.A0A1A0H567"/>
    <dbReference type="GeneID" id="30028142"/>
    <dbReference type="OrthoDB" id="3251668at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="16652" checksum="69B46839D9DD5F69" modified="2016-10-05" version="1">MEQQAWVHSVFSREVSVCQLLGPSSPMLVFQCQATRPSSKEFLKKEKQTTCKKKTKGSTFIRREKVKKEKRQRRSKQGCWTCRLRHKACPEDGDPCGACARLGLECDVSRQRPSYMVDAQNAMHRLKEIRAVTDKLRKNRFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5M7</accession>
    <name>A0A1A0H5M7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_13683</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the large ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 32 different proteins and 1 molecule of RNA (18S). The 60S subunit contains 45 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709926.1">
        <property type="nucleotide sequence ID" value="XM_018854411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5M7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5M7"/>
    <dbReference type="GeneID" id="30027387"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="8">
        <location>
            <begin position="120"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="265" mass="29050" checksum="E2C6622165005369" modified="2016-10-05" version="1">MAPKGKKVAPAPLATKSESSKAPKNPLFETTPKNYGIGQSIQPKRNLTRYVKWPQYVRLQRQKKILNMRLKVPPAIAQFQNVLDKNTATQTFKLFNKYRPETAAEKKDRLTKEAAAIAEGKKAFDVSAKPVVVKYGLNHVVSLIENKKAKLVLIANDVDPIELVIFLPALCRKMGVPYAIVKGKARLGTLVHKKTSSVAALTEVAAADEADLAKLVSTINANFIEKYEDSRKHWGGGIMGKKHNDKVIKKAKQMDVAAAPAAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H616</accession>
    <name>A0A1A0H616_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNase I-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_45970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710057.1">
        <property type="nucleotide sequence ID" value="XM_018857976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H616"/>
    <dbReference type="GeneID" id="30030952"/>
    <dbReference type="OrthoDB" id="276515at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09083">
        <property type="entry name" value="EEP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF36">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="76"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="336" mass="36990" checksum="6F6AB3AF434BAC18" modified="2016-10-05" version="1" fragment="single">PGLPGSALTAPQSVAVVPEPAYPEIAGPSPQTAAGHVAFRFYSHNVKNGLHDDLVPGEMPWDMRKRLVINSIKFNAVANSVVFLQEPLKFQLDDILADLNYLSAPGTPEWVAVGSGRVDGKDVGEHVPILFRPAEWTVVFNDTFWLNDGATRMAVAGWDAKYPRIATYATLKHALSGHHINVANTHFDHKGKTARRESALLLVAKLRSLNLWPLFVMGDLNSQPGDASHREMTKAFVDVHDHVPDFSRYGHLDYTVTGFSGKYLKDAKRIDYIFAPKDTRRLSEPMCADPALPYSLQVVGYGLLHSKFGGVYMSDHRPVVADFVMKGCDLGQATDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H639</accession>
    <name>A0A1A0H639_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA mismatch repair protein MutL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33551</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709915.1">
        <property type="nucleotide sequence ID" value="XM_018856329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H639"/>
    <dbReference type="STRING" id="869754.A0A1A0H639"/>
    <dbReference type="GeneID" id="30029305"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032390">
        <property type="term" value="C:MutLbeta complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097587">
        <property type="term" value="C:MutLgamma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000713">
        <property type="term" value="P:meiotic heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair endonuclease MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000014">
        <property type="entry name" value="DNA mismatch repair protein Mlh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032189">
        <property type="entry name" value="Mlh1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF12">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16413">
        <property type="entry name" value="Mlh1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="238"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="712" mass="80453" checksum="84F7739178856518" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H697</accession>
    <name>A0A1A0H697_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UBR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_46278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709960.1">
        <property type="nucleotide sequence ID" value="XM_018858022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H697"/>
    <dbReference type="GeneID" id="30030998"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19677">
        <property type="entry name" value="UBR-box_UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040204">
        <property type="entry name" value="UBR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047506">
        <property type="entry name" value="UBR7-like_UBR-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13513:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="6">
        <location>
            <begin position="35"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="4">
        <location>
            <begin position="35"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="391" mass="42601" checksum="C04F7CFBEAF1997A" modified="2016-10-05" version="1">MPESPADSSLTAADFLEHQAQLEQEAREAMPYEPDTCTYPRPLRQHVFACLTCRRASAAAVGVCYLCLIQCHSTHDLVELFSKRHFACDCGTGRMAGGAACAVRARATASGGSRPFSAPPLPPASDIESLGNSYNHNYAGRFCSCAAPYDPALERRTMHQCYLGDACGEDWYHQECILGYVPGLFLGPRAHAVPHFPDEDAFVEFVCWRCVQRHGRAFAELVACAGVVLATRPHLAGADSADTWRSLAESGPGEKGVKRPRRSTLPRPRPAPYSVFLAPGFHARLSALRGRLPPGSALRKLLEAFGFLVTEEPVHQPAADGAASTPAAEGSLYDLGSTALRSLPIPQALEGLEAYDAMKEKLRGFFKDFVEKKKVVTEEEVRAFFGRMEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6A1</accession>
    <name>A0A1A0H6A1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="5">2.3.3.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_45953</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710084.1">
        <property type="nucleotide sequence ID" value="XM_018857975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6A1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6A1"/>
    <dbReference type="GeneID" id="30030951"/>
    <dbReference type="OrthoDB" id="418791at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000045">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054692">
        <property type="entry name" value="LeuA-like_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002991">
        <property type="entry name" value="PRK03739.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46911:SF1">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22615">
        <property type="entry name" value="IPMS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="11">
        <location>
            <begin position="33"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="574" mass="63508" checksum="93231AE9949B2580" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6K3</accession>
    <name>A0A1A0H6K3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAR-17a/AIG1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_45678</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710118.1">
        <property type="nucleotide sequence ID" value="XM_018857946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6K3"/>
    <dbReference type="GeneID" id="30030922"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25866" checksum="3A879FFD04A734DD" modified="2016-10-05" version="1">MARTAGDFRAIGLNLAAVASGIYAVHYISTYVELPPHLAGAGHWQFLTNISLLLSQIVFALGGVAHFFKLDSLFRLKNNLHPIALAVECIVTGIYWPLRIMFIHILVKDPNAKFIPLSVDLCLHLVPVLGLAIDYFFFMPKWTVLPRFSLGACVVLAGLYWWWLKIIIDFENGGEYPYMFLNVPDELTRVLIFGVVALLAFALFMTLRLMHDLLICADITEDEKLKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6N9</accession>
    <name>A0A1A0H6N9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_33271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710153.1">
        <property type="nucleotide sequence ID" value="XM_018856257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6N9"/>
    <dbReference type="GeneID" id="30029233"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="54" mass="6170" checksum="A7E113907B2FFAC0" modified="2016-10-05" version="1" fragment="single">MGETTMSAETNKDTSTLTFNPTMYFSDAEQDRAAQEYLSNMNRLLETKICPLQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6P9</accession>
    <name>A0A1A0H6P9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Multidrug efflux transporter AcrB transmembrane domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_73968</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the patched family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710163.1">
        <property type="nucleotide sequence ID" value="XM_018858420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6P9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6P9"/>
    <dbReference type="GeneID" id="30031396"/>
    <dbReference type="OrthoDB" id="6510177at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015918">
        <property type="term" value="P:sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1640.10:FF:000029">
        <property type="entry name" value="Putative Patched sphingolipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053958">
        <property type="entry name" value="HMGCR/SNAP/NPC1-like_SSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053956">
        <property type="entry name" value="NPC1_MLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032190">
        <property type="entry name" value="NPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45727:SF2">
        <property type="entry name" value="NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22314">
        <property type="entry name" value="NPC1_MLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16414">
        <property type="entry name" value="NPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12349">
        <property type="entry name" value="Sterol-sensing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291683" evidence="12">
        <location>
            <begin position="19"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="587"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="652"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="701"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="734"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1098"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1129"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1183"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1213"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="13">
        <location>
            <begin position="584"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1239" mass="138757" checksum="434331EAFBFD09FA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6W5</accession>
    <name>A0A1A0H6W5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleoside phosphatase GDA1/CD39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79649</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710180.1">
        <property type="nucleotide sequence ID" value="XM_018859105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6W5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6W5"/>
    <dbReference type="GeneID" id="30032081"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004382">
        <property type="term" value="F:GDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045134">
        <property type="term" value="F:UDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046036">
        <property type="term" value="P:CTP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006256">
        <property type="term" value="P:UDP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782:SF121">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATASE MIG-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="94"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="248"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="80502" checksum="9DE65FC996777491" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6X3</accession>
    <name>A0A1A0H6X3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_207789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710306.1">
        <property type="nucleotide sequence ID" value="XM_018855006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6X3"/>
    <dbReference type="GeneID" id="30027982"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="166" mass="19013" checksum="C048E0303D95865E" modified="2016-10-05" version="1">MAPRPCFLGFGGFFAAPGPPCRISPYRASFGVTCSLAFSPLLYLKIVLNYLNSNSPSFLSPLSTQPLPRNYFHLEPRVFRKSLSNFTSPPRNLYTGSLNWLFFFRHRNTPVALSCYQAFKKFLRVVFQPSFVDFTATPFAACQHRLDFWRAPSDSTLHFHLPLYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H704</accession>
    <name>A0A1A0H704_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710215.1">
        <property type="nucleotide sequence ID" value="XM_018858008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H704"/>
    <dbReference type="STRING" id="869754.A0A1A0H704"/>
    <dbReference type="GeneID" id="30030984"/>
    <dbReference type="OrthoDB" id="4020988at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="555" mass="62298" checksum="ED6298DCA68B3A00" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7T4</accession>
    <name>A0A1A0H7T4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UBC core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_45028</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710684.1">
        <property type="nucleotide sequence ID" value="XM_018857862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7T4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7T4"/>
    <dbReference type="GeneID" id="30030838"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23811">
        <property type="entry name" value="UBCc_ScCDC34-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="5"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="193"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="195"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="238" mass="27122" checksum="9CB9287E54F1A8B6" modified="2016-10-05" version="1">MSSKSAAAILQRQFKDLTDPKKGIPYFHIELDNDNIFYWKIGIMVLNKESLYYGGYFKGRMRFPADYPFSPPSFAFTPPIFHPNVYKDGRLCISILHEGGDPISGMPDAETWSPAQTAESVLISIISLLEDPNLNSPANMDACWLFRKNFESYKEKVLDGVEKSKKDIPKGFVFPDPDDSALSGNAANKELEQAQYDEEFWYDSDEESFDEESLMDDINDEEDDEVEEEDDSDVEMGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Z2</accession>
    <name>A0A1A0H7Z2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_78673</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710741.1">
        <property type="nucleotide sequence ID" value="XM_018858915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Z2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Z2"/>
    <dbReference type="GeneID" id="30031891"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="42"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="262" mass="29209" checksum="D299E3B62A6FA4B9" modified="2016-10-05" version="1">MARGPKKHLKRLAAPSHWMLDKLSGTYAPRPSAGPHKLRESLPLIVFLRNRLKYALNGREVKAILMQEHVKVDGKVRTDSTFPAGFMDVITLEATNENFRLVYDVKGRFTVHRITGEEASYKLGKVRKIALGKRGVPYAVTHDGRTIRYPDPQVKANDTVKIDLATGKITDFIKFDTGKLAMVTGGKNMGRVGVIVHREKHEGGFDLVHIKDALENTFVTRQSNVFVIGTEAGKPYVSLPKGKGIKLSITEERDRRRAQGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H828</accession>
    <name>A0A1A0H828_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710572.1">
        <property type="nucleotide sequence ID" value="XM_018854300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H828"/>
    <dbReference type="GeneID" id="30027276"/>
    <dbReference type="OrthoDB" id="4010386at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012470">
        <property type="entry name" value="Pup1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07954">
        <property type="entry name" value="DUF1689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="32814" checksum="994277CE9B5B2DC3" modified="2016-10-05" version="1">MTEKDFEQDSFSSEDHHDPMILGKKIKKDDFEHLMGFYEADHKLNTEERHIMLKDLTSSGVTTMIWALIDSSIVVTTPFIYRRYQEKKLGGPLPVLPRSTAFLKLIFFLVAGISVYFWSSKYHAKNDQQYRVANLEADCSSGKFSEQEIESKQRLLNVWKSLDPRNLSLFTVYYYKSASDPSLVMKSPKDMVSFDPHAVTYLPPPQKNGQLQGVLGAVKEHEKQAPHWQKIREVNGFVSPTDDGSDSSASIDDSQVLDDARAAEDPAFEQWKEPAAKTSAWDRLRKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H854</accession>
    <name>A0A1A0H854_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_79374</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SF3B4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710597.1">
        <property type="nucleotide sequence ID" value="XM_018859052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H854"/>
    <dbReference type="STRING" id="869754.A0A1A0H854"/>
    <dbReference type="GeneID" id="30032028"/>
    <dbReference type="OrthoDB" id="10259687at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12334">
        <property type="entry name" value="RRM1_SF3B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000895">
        <property type="entry name" value="Hsh49p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000505">
        <property type="entry name" value="Splicing factor 3B subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034158">
        <property type="entry name" value="SF3B4_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="16"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="112"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="231" mass="26425" checksum="7B332D65BA72B5B9" modified="2016-10-05" version="1">MNVFRKSLEQDRNVHATIYVGNLDTQVTEALLYELFIQVGPVRLLYLPKDRVLRTHQGFGFVEFRGIEDADFALNLLRGVRLYGRTLKMKKTDPQSNSSNSPEFKHTAGVGARLFIKNLHQLVDEKYLQNTFSAIGNLINQPIIIRDEKGHSKGYGFIEFGDFESSDKAIERLNGAIFMNNRITLSYAYKEGQEGKKIQHGDHAERTLAKQGNKHLKKEPATRETRSSRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8J0</accession>
    <name>A0A1A0H8J0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="6">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_44006</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Vacuole membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710724.1">
        <property type="nucleotide sequence ID" value="XM_018857734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8J0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8J0"/>
    <dbReference type="GeneID" id="30030710"/>
    <dbReference type="OrthoDB" id="1735853at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="9">
        <location>
            <begin position="33"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="8">
        <location>
            <begin position="153"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="10">
        <location>
            <begin position="385"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="490"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="509" mass="56807" checksum="7740420A82DD7810" modified="2016-10-05" version="1">MLSDKELFDLNKKAVSEGFKIKPRINYNTVGGVNGPLVILDNVKFPRFNEIVNLTLPDGTVRAGQVLEVRGLKAIVQVFEGTSGIDIKKTRVEFTGENLKISVSEDMLGRVFDGSGRPIDKGPKIFAEDYLDINGSPINPYARIYPEEMISTGVSAIDTMNSIARGQKIPIFSASGLPHNEIAAQICRQAGLVRPTKDVHDGHEDNFSIVFAAMGVNLETSRFFKQDFEENGSLDRTSLFLNLANDPTIERIITPRLALTTAEYLAYQTERHVLTILTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFVDRQLDNRGIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYAKYAIGRDAAAMKSVVGEEALSTEDKLALEFLEKFEKNFIAQGAYEDRSIFDSLDLAWSLLRIFPKEMLNRISPKILDEFYDRSSEQDEDDEDDENPDQSHDLIDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9N4</accession>
    <name>A0A1A0H9N4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ada DNA repair metal-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_15944</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711248.1">
        <property type="nucleotide sequence ID" value="XM_018854621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9N4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9N4"/>
    <dbReference type="GeneID" id="30027597"/>
    <dbReference type="OrthoDB" id="2447880at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="entry name" value="DNA Methylphosphotriester Repair Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004026">
        <property type="entry name" value="Ada_DNA_repair_Zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02805">
        <property type="entry name" value="Ada_Zn_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="Ada DNA repair protein, N-terminal domain (N-Ada 10)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ada DNA repair metal-binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="282"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="180"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="327" mass="35155" checksum="CADE4BAE507AD238" modified="2016-10-05" version="1" fragment="single">MVYSTDESKWRAYQFLDPFAAGSFVVCNKINSYFCRPDCDAHPITELHLEIKFVESPNDAARLGFSPCKSCDPMLVPSIDVNLLLVTVKEINAMIGFVPPLLDDDENKVTETIKENIMCQSSVQRRMSVPANAFSNKTATSLKPLAGAVSKNDSEHYRLIDLACRHLALAAAMSIFNGPGASAGSSPRSDDSSSGGAKKKGGKKRRGGVLGFKELATKSKLSAWHFHRVFKSIIGLTPKTYGDMCWEFLEKEKDAMDAGETPARKPSSVSLLADSERLAVGGLEHGSTSGSVAGLGPVSEPAPLSMAPAATTPQLSEEPCPFELPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9X3</accession>
    <name>A0A1A0H9X3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">SRF-TF-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_44130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711199.1">
        <property type="nucleotide sequence ID" value="XM_018857746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9X3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9X3"/>
    <dbReference type="GeneID" id="30030722"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00350">
        <property type="entry name" value="MADS_BOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="50"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="233" mass="25290" checksum="E65EABD5E373A3AC" modified="2016-10-05" version="1">MSEITDPSKLEGSDETLAGAPGPQFNRGSVSGLGGDDDDDDEPAGGKQQKERRKIEIKFIQDKSRRHITFSKRKAGIMKKAYELSVLTGTQVLLLVVSETGLVYTFTTPKLQPLVTKSEGKNLIQACLNAPEEGLGDEQGDQSEANSPELPEQSPAPQKHHAPVANVAHQVPPPAVPHPQQLPPTHLPHGMPYQGQGHPQQGLPMPQAGGYNDPYQYFGNMGNGNMQNQQQYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA52</accession>
    <name>A0A1A0HA52_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_205582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711275.1">
        <property type="nucleotide sequence ID" value="XM_018854974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA52"/>
    <dbReference type="GeneID" id="30027950"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291711" evidence="1">
        <location>
            <begin position="23"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="135" mass="15404" checksum="69DC56765A8E61BC" modified="2016-10-05" version="1">MKLERNSVFLALTLLGTVTSRAVFFTVNSDDQSIILVIEEEDSRIISSQRSGAVKTHFYGEKLECKGNYLSVENEMLALSKTPKNYLTTILDKDDRFPHLEAKGDGVFYVCEDRLIHLKDGCPGAQEVKIFYYEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA53</accession>
    <name>A0A1A0HA53_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_205607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711279.1">
        <property type="nucleotide sequence ID" value="XM_018854976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA53"/>
    <dbReference type="GeneID" id="30027952"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="13198" checksum="F24D5C648E98AA5E" modified="2016-10-05" version="1">MISRTDPHVGQERACQGAPSKMRQNRLSATAPFFSVRNAAFAGRPFRVVVTCAYQFVVFAGAYVGTPGEYSQDVFGWQIGHDDSARYCACHSPNVWSQGLPTRCKQSGGTVGPGSGDATHVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAE0</accession>
    <name>A0A1A0HAE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent DNA helicase II subunit Ku70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_41288</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711480.1">
        <property type="nucleotide sequence ID" value="XM_018857378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAE0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAE0"/>
    <dbReference type="GeneID" id="30030354"/>
    <dbReference type="OrthoDB" id="3249161at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006165">
        <property type="entry name" value="Ku70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005160">
        <property type="entry name" value="Ku_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF2">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03730">
        <property type="entry name" value="Ku_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003033">
        <property type="entry name" value="Ku70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="18">
        <location>
            <begin position="318"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA20970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="703" mass="81168" checksum="918EEDF67A42DB4A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAF9</accession>
    <name>A0A1A0HAF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_54905</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711372.1">
        <property type="nucleotide sequence ID" value="XM_018858293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAF9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAF9"/>
    <dbReference type="GeneID" id="30031269"/>
    <dbReference type="OrthoDB" id="6770063at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF48">
        <property type="entry name" value="MULTIDRUG TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G02700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="424"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="536"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="154"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="596" mass="66290" checksum="205D2D1623B32C10" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAN3</accession>
    <name>A0A1A0HAN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Chitin synthase</fullName>
            <ecNumber evidence="2 10">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_42164</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chitin synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 10"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711448.1">
        <property type="nucleotide sequence ID" value="XM_018857506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAN3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAN3"/>
    <dbReference type="GeneID" id="30030482"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="623"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="667"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="698"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="749"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="875"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="912"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="11">
        <location>
            <begin position="189"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="951" mass="108434" checksum="7E3B12B219796173" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAW7</accession>
    <name>A0A1A0HAW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LAA1-like C-terminal TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_42355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711666.1">
        <property type="nucleotide sequence ID" value="XM_018857533.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HAW7"/>
    <dbReference type="GeneID" id="30030509"/>
    <dbReference type="OrthoDB" id="192608at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040108">
        <property type="entry name" value="Laa1/Sip1/HEATR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046837">
        <property type="entry name" value="Laa1/Sip1/HEATR5-like_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057981">
        <property type="entry name" value="TPR_LAA1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663:SF0">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21663">
        <property type="entry name" value="HYPOTHETICAL HEAT DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20210">
        <property type="entry name" value="Laa1_Sip1_HTR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25808">
        <property type="entry name" value="TPR_LAA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LAA1-like C-terminal TPR repeats" evidence="2">
        <location>
            <begin position="1939"/>
            <end position="2099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2110" mass="236770" checksum="94C5EBDEE3831AF6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAW9</accession>
    <name>A0A1A0HAW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711779.1">
        <property type="nucleotide sequence ID" value="XM_018857387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAW9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAW9"/>
    <dbReference type="GeneID" id="30030363"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16473">
        <property type="entry name" value="RING-H2_RNF103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF416">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE GODZILLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="238"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="439"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="481"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="515" mass="56533" checksum="2FB5347B0898416E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAZ8</accession>
    <name>A0A1A0HAZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_55357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711567.1">
        <property type="nucleotide sequence ID" value="XM_018858295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAZ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HAZ8"/>
    <dbReference type="GeneID" id="30031271"/>
    <dbReference type="OrthoDB" id="3995760at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="89"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="750" mass="84777" checksum="477A0CC1DA0C53C8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB61</accession>
    <name>A0A1A0HB61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_42191</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the NDC80 complex, which consists of NDC80, NUF2, SPC24 and SPC25.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Chromosome</location>
            <location evidence="11">Centromere</location>
            <location evidence="11">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711638.1">
        <property type="nucleotide sequence ID" value="XM_018857509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB61"/>
    <dbReference type="STRING" id="869754.A0A1A0HB61"/>
    <dbReference type="GeneID" id="30030485"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.30:FF:000001">
        <property type="entry name" value="Probable kinetochore protein ndc80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="10 11" id="KW-0137">Centromere</keyword>
    <keyword evidence="3 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="14">
        <location>
            <begin position="72"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="281"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="621" mass="71196" checksum="47DBA8CE9C92F4C1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB62</accession>
    <name>A0A1A0HB62_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_229614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711627.1">
        <property type="nucleotide sequence ID" value="XM_018855229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB62"/>
    <dbReference type="GeneID" id="30028205"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="406"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1187" mass="131856" checksum="BD50126334EE1BDA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBB6</accession>
    <name>A0A1A0HBB6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="14">TC-AMP synthase</shortName>
            <ecNumber evidence="3 14">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 14">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_78220</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Likely catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Required for normal translation, by ensuring translation fidelity at the level of codon recognition, appropriate translation initiation selection and maintenance of reading frame. Also involved in telomere replication. Binds to single-stranded telomeric (ssTG) DNA and positively regulates telomere length.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the SUA5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3 14"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711688.1">
        <property type="nucleotide sequence ID" value="XM_018858846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBB6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBB6"/>
    <dbReference type="GeneID" id="30031822"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000008">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="16">
        <location>
            <begin position="28"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA21178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="372" mass="40382" checksum="4F6A2992B849E412" modified="2016-10-05" version="1">MHTKILAVLKHSISFLNEKPIFNDENTEKDMRMAASAVRQAGKVVAFPTETVYGLGGSALNDQSVKAIYQAKNRPADNPLIVHVSSIEQVQRVILTELHKIPPVYEKLLQRFWPGPLTILVPIEKDCPISELVTTGQKTVGVRMPDHSIARALIALSDTPLAAPSANASTRPSPTLASHVMFDLNGKIPYILDGGACSVGVESTVIDGLCSPPMLLRPGGVSAEDIKKYGGEEWKNLVIAKKSAGKSESVRTPGMKYKHYSPTAKVILFVNCGDGAESVRHFLKKKEGKIAILKSTRFLLAQEMGVSNVIERELGNSATEIAHSLFRELRDVDELGVDYILVEGVEEVGDGLAVMNRLSKAASDIIEGDYKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS6</accession>
    <name>A0A1A0HBS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Profilin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_41082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the profilin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712099.1">
        <property type="nucleotide sequence ID" value="XM_018857351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS6"/>
    <dbReference type="GeneID" id="30030327"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003785">
        <property type="term" value="F:actin monomer binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00148">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.30:FF:000001">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048278">
        <property type="entry name" value="PFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005455">
        <property type="entry name" value="PFN_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036140">
        <property type="entry name" value="PFN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027310">
        <property type="entry name" value="Profilin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11604:SF0">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00235">
        <property type="entry name" value="Profilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00392">
        <property type="entry name" value="PROFILIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01640">
        <property type="entry name" value="PROFILINPLNT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00392">
        <property type="entry name" value="PROF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55770">
        <property type="entry name" value="Profilin (actin-binding protein)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00414">
        <property type="entry name" value="PROFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="126" mass="13615" checksum="5A23728A2460F238" modified="2016-10-05" version="1">MSWNAYTDSLLSTGKIDKAAIYSVAGDSLWAQSGGFQIAPQEITNIAGGYADASQLQAHGLRVEGEKYFLLKSDERSIYGKQEDRGIIAVKTKQAILLAHYPSGVQAQEATAVVEKLADYLISLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC45</accession>
    <name>A0A1A0HC45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">assimilatory sulfite reductase (NADPH)</fullName>
            <ecNumber evidence="5">1.8.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_32058</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogen sulfide + 3 NADP(+) + 3 H2O = sulfite + 3 NADPH + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.2" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712065.1">
        <property type="nucleotide sequence ID" value="XM_018855978.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HC45"/>
    <dbReference type="GeneID" id="30028954"/>
    <dbReference type="OrthoDB" id="1688044at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000097">
        <property type="term" value="P:sulfur amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000003">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.413.10:FF:000004">
        <property type="entry name" value="Sulfite reductase [NADPH] hemoprotein beta-component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000016">
        <property type="entry name" value="Sulfite reductase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="entry name" value="Sulfite Reductase Hemoprotein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010029">
        <property type="entry name" value="PRK13504.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493:SF47">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="SULFITE REDUCTASE [NADPH] SUBUNIT BETA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="Nitrite and sulphite reductase 4Fe-4S domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="Nitrite/Sulfite reductase N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="15">
        <location>
            <begin position="664"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1010"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1011"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1421" mass="157033" checksum="1F0346B645A8395B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG6</accession>
    <name>A0A1A0HCG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_12165</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712195.1">
        <property type="nucleotide sequence ID" value="XM_018854185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCG6"/>
    <dbReference type="GeneID" id="30027161"/>
    <dbReference type="OrthoDB" id="1162399at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990328">
        <property type="term" value="C:RPB4-RPB7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010590">
        <property type="term" value="P:regulation of septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04462">
        <property type="entry name" value="S1_RNAPII_Rpb7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="173" mass="19092" checksum="D7F58D1E649172AF" modified="2016-10-05" version="1">MFFLKDLSLNLTLHPSYFGPQMDQYLRDKLLSDVEGTCTGQFGYIVCVLDCMNIDVGRGRVIPGSGLAEFEVKYRAVVWKPFKGEVVDGVVTTVNKMGFFADVGPLSVFVSTHLIPSDMKFNPSANPPAYVSPDETIEKGSRVRLKIVGTRTDVNEIYAIGSIKEDYLGPSPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCK5</accession>
    <name>A0A1A0HCK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">VHS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_78532</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in the regulation of membrane traffic through the trans-Golgi network.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712132.1">
        <property type="nucleotide sequence ID" value="XM_018858894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCK5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCK5"/>
    <dbReference type="GeneID" id="30031870"/>
    <dbReference type="OrthoDB" id="2018246at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14235">
        <property type="entry name" value="GAT_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16998">
        <property type="entry name" value="VHS_GGA_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000008">
        <property type="entry name" value="VHS domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000024">
        <property type="entry name" value="VHS domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052653">
        <property type="entry name" value="ARF-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008153">
        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47180:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02883">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03127">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00809">
        <property type="entry name" value="Alpha_adaptinC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50180">
        <property type="entry name" value="GAE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50909">
        <property type="entry name" value="GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VHS" evidence="7">
        <location>
            <begin position="24"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="GAT" evidence="9">
        <location>
            <begin position="187"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="GAE" evidence="8">
        <location>
            <begin position="463"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="577" mass="62897" checksum="0D8865B832A15202" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCM1</accession>
    <name>A0A1A0HCM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UDP-glucose:glycoprotein glucosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_77411</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712350.1">
        <property type="nucleotide sequence ID" value="XM_018858695.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HCM1"/>
    <dbReference type="GeneID" id="30031671"/>
    <dbReference type="OrthoDB" id="27683at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003980">
        <property type="term" value="F:UDP-glucose:glycoprotein glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040497">
        <property type="entry name" value="Glyco_transf_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009448">
        <property type="entry name" value="UDP-g_GGtrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040693">
        <property type="entry name" value="UGGT_TRXL_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040694">
        <property type="entry name" value="UGGT_TRXL_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040692">
        <property type="entry name" value="UGGT_TRXL_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226">
        <property type="entry name" value="UDP-GLUCOSE GLYCOPROTEIN:GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11226:SF0">
        <property type="entry name" value="UDP-GLUCOSE:GLYCOPROTEIN GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18404">
        <property type="entry name" value="Glyco_transf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18400">
        <property type="entry name" value="Thioredoxin_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18401">
        <property type="entry name" value="Thioredoxin_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18402">
        <property type="entry name" value="Thioredoxin_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06427">
        <property type="entry name" value="UDP-g_GGTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291790" description="UDP-glucose:glycoprotein glucosyltransferase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="7">
        <location>
            <begin position="31"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="8">
        <location>
            <begin position="276"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="UGGT thioredoxin-like" evidence="9">
        <location>
            <begin position="435"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="Glucosyltransferase 24 catalytic" evidence="10">
        <location>
            <begin position="1142"/>
            <end position="1409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1420" mass="161121" checksum="D354932D66E70107" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCQ0</accession>
    <name>A0A1A0HCQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PapD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_25371</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712370.1">
        <property type="nucleotide sequence ID" value="XM_018855292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCQ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCQ0"/>
    <dbReference type="GeneID" id="30028268"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="MSP" evidence="6">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="124" mass="13827" checksum="2E1F31DC9637A3DD" modified="2016-10-05" version="1" fragment="single">LTHPGTFAEPLTEYLTLTNTTPEPLAFKVKTTAPKLYCVRPNANIVAPGESMKISVILQGYTTPLPKDFKCKDKFLIVSCPCPGFEDASKVAENWAALYQEHGQQGVQKKLRVNFIIEEDVRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCZ0</accession>
    <name>A0A1A0HCZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enolase-phosphatase E1</fullName>
            <ecNumber evidence="6">3.1.3.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2,3-diketo-5-methylthio-1-phosphopentane phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">UTR4</name>
        <name evidence="7" type="ORF">METBIDRAFT_10729</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="3.1.3.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.77" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712299.1">
        <property type="nucleotide sequence ID" value="XM_018853837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCZ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCZ0"/>
    <dbReference type="GeneID" id="30026813"/>
    <dbReference type="OrthoDB" id="272500at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00876"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03117">
        <property type="entry name" value="Salvage_MtnC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027511">
        <property type="entry name" value="ENOPH1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="121"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="228" mass="24971" checksum="5A7696AC851A32EB" modified="2016-10-05" version="1">MVALLDIEGTVCSISFVKETLYPYFLETYASTISTMEYPITGERDEMSAILSGFPTEAKQSQEALKSHISHLVSNDIKDPVLKAFQGKVWKLGYAKGELKAPVYSDAIEFFERSDKTYIYSSGSVAAQKLLFAHVDVNGCLTDLTPKLAGYFDITTSGYKQEQASYASISSAIGVAPKDITFYSDNINEIKAAHSAGLATRLVVRPGNEPVSEQDKAVFTVITTFDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD60</accession>
    <name>A0A1A0HD60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_39511</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fmt family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712401.1">
        <property type="nucleotide sequence ID" value="XM_018857148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD60"/>
    <dbReference type="STRING" id="869754.A0A1A0HD60"/>
    <dbReference type="GeneID" id="30030124"/>
    <dbReference type="OrthoDB" id="10268103at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11138:SF5">
        <property type="entry name" value="METHIONYL-TRNA FORMYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="5">
        <location>
            <begin position="34"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl transferase C-terminal" evidence="6">
        <location>
            <begin position="213"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="342" mass="37437" checksum="17760F7F6736A82B" modified="2016-10-05" version="1">MPSGLRIAFFGLDLFSVASLRKLAELHRSRPGLISSLDVYTRSIKPTGRNLKQFVDLPIGTYALGNGLRVFRVDSSAEIVASALPQNYDLAVAVSYGKLIPGKFLASLRYGGLNVHPSLLPKFSGSSPIQHALMEDCKSTGVTVQTLHPTKFDHGDIICQSEEVEILNDDKFVSLQEKLATVGADLLADVVYSGSYTNPPHFQSLEQYSLAPKLSPAKSEVKWGQMSSRQVRRLNDALGPLHSYKYVDILKKKKPLQGNFKVILDDIHELLTCAELDSLSTPGTFTLSADGLVVKTSDSYVSVKKLKFQYCGLESPAEFMKNLKKRSGTTACQFISSDASSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDA4</accession>
    <name>A0A1A0HDA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_77517</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712492.1">
        <property type="nucleotide sequence ID" value="XM_018858713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDA4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDA4"/>
    <dbReference type="GeneID" id="30031689"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="515"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="579"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="191"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="601" mass="67312" checksum="1C9099995635BB11" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDB6</accession>
    <name>A0A1A0HDB6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_140863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712419.1">
        <property type="nucleotide sequence ID" value="XM_018854480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDB6"/>
    <dbReference type="GeneID" id="30027456"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20286" checksum="B60320C2A890A96E" modified="2016-10-05" version="1">MSLQQHQPISHTAFIPANLKYSVHSSQSHIQRSFQPISHTAFIPANLKYSVHSSQSEIRSFQPISNTFIPANLKYSVHSSLFDKSDSANFDRVCPPISHTAFIPANIDKSDPANFDSSLSSADSSQRRQIGFRQLQQNSPHNQPGQPPTPNQNHRRGRHKRAACPGFPDFQVPPQRPRLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDD1</accession>
    <name>A0A1A0HDD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_40458</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Vacuole membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712585.1">
        <property type="nucleotide sequence ID" value="XM_018857276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDD1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDD1"/>
    <dbReference type="GeneID" id="30030252"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000003">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <keyword evidence="4 12" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="13">
        <location>
            <begin position="96"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="160" mass="16366" checksum="86AA4BE83DA6C765" modified="2016-10-05" version="1">MSTSDELNPLFAPFLGLAGCAASMVFSCVGAAVGSAKSGIGISGIGTFKPELIMKSLIPVVLSGILSVYGLVVSVLIAGGLNPAEDYSLFKGIMHLACGLSVGLACMASGYAIGIVGDEGVRQYMHQPRLFVGIVLILIFAEVLGLYGLIIALILNTKGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDI3</accession>
    <name>A0A1A0HDI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_40264</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 8">Nucleus</location>
            <location evidence="3 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712531.1">
        <property type="nucleotide sequence ID" value="XM_018857250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDI3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDI3"/>
    <dbReference type="GeneID" id="30030226"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="10">
        <location>
            <begin position="213"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="473"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="544" mass="61999" checksum="624C03C1DBE5E06E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK2</accession>
    <name>A0A1A0HDK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712660.1">
        <property type="nucleotide sequence ID" value="XM_018855771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDK2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDK2"/>
    <dbReference type="GeneID" id="30028747"/>
    <dbReference type="OrthoDB" id="3998161at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="529" mass="58559" checksum="1660A0EBC67DBAE4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDN3</accession>
    <name>A0A1A0HDN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712622.1">
        <property type="nucleotide sequence ID" value="XM_018855766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDN3"/>
    <dbReference type="GeneID" id="30028742"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="81" mass="9217" checksum="6BD47505460FCFD8" modified="2016-10-05" version="1" fragment="single">VFTLYTRHLYVTRISPPKIIAAPQFRIWSPTTLLSVALCSLTNVDPTGNSASCMVWPQPIFYIEDTLRSLKVTLFCFCKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDQ8</accession>
    <name>A0A1A0HDQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Histone deacetylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_77674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712715.1">
        <property type="nucleotide sequence ID" value="XM_018858743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDQ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDQ8"/>
    <dbReference type="GeneID" id="30031719"/>
    <dbReference type="OrthoDB" id="5232919at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053244">
        <property type="entry name" value="HDAC_HD_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47558:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE HOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="208"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="503"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="712" mass="82008" checksum="AF0643511C5AE309" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDR5</accession>
    <name>A0A1A0HDR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_40019</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712533.1">
        <property type="nucleotide sequence ID" value="XM_018857220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDR5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDR5"/>
    <dbReference type="GeneID" id="30030196"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="397"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="705" mass="82178" checksum="DE585F5045B9EE13" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDS3</accession>
    <name>A0A1A0HDS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Translocase of outer membrane 40 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_77571</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Channel-forming protein essential for import of protein precursors into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712569.1">
        <property type="nucleotide sequence ID" value="XM_018858724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDS3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDS3"/>
    <dbReference type="GeneID" id="30031700"/>
    <dbReference type="OrthoDB" id="19656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07305">
        <property type="entry name" value="Porin3_Tom40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000009">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00078731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="42026" checksum="CF0C82A21FCD3ED4" modified="2016-10-05" version="1">MSDTQQTSELSQIAIPTLPSLAPELPKQNGLWSSNPVFSYLNDVYINIAEHRKSLGLTNPGTIENLTKEVNRDVFLSQYFFTGLRADLNKAFSLDPAFQTSHTLSMGSNVLPPYAFSALYARENFFVQGNVDNDFSFSGKVNYGWDAHNILKLTLQIAHGQPVMCQLEQDYQANDFSVNVKSLNPSLSNGGFSGVAVASLLQSLTPKFAVGLEAMYSRQAMSLPPDSAVSYFGRYNAGKWIATAQVQAQGALVGTFWRKVSDRVEAGLETQLMASVRPITDSLMGTPIGYEPVVEGQTTIGAKYEYRQSVFRGQLDTLGKVGVFMEKRVLPTVSILFSGEIDHRKNASKLGVGLQFESAGNEQLMLMQQGLVDANGNPVPGAPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE18</accession>
    <name>A0A1A0HE18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_77619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712644.1">
        <property type="nucleotide sequence ID" value="XM_018858733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE18"/>
    <dbReference type="STRING" id="869754.A0A1A0HE18"/>
    <dbReference type="GeneID" id="30031709"/>
    <dbReference type="OrthoDB" id="10256055at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677:SF1">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41677">
        <property type="entry name" value="YALI0B19030P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="399" mass="43886" checksum="619FBBD30A1DF75F" modified="2016-10-05" version="1">MAPPELAADIEAGNKRKAISEDTYERCLAGTFAALSARLGQPHAVSFDPLVHLKYYSQGPAEQHKYNHTRRITMEELGLTSKTQISPVGVSDPFPLFTDEAVDIMRWEILHRDTFLANARLSYNSTSGLDCAMRGYVSDGRTVHTPFTHAAWTHPETMQLISTMAGVDLQVVMDHEIAHVNVGITDPHTAARQRSLHEAEQRERLFRGTQKDADADAPGPAGDAEIPAIVGWHHDAYPFVCVLMLSDTTDMVGGETYLRMGDGQLARVAGPQKGHAAVLQGRLIRHLAPKPVGATERITMVTSYRARDAAQHDGSVLGTVKPEINYGSKYSDFYRQWVGYRAEVIKARLDRLTASMGHGTVFDKAAATAGLQDIEAYLAATYTEMEVTADEWREVVGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE52</accession>
    <name>A0A1A0HE52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="10">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31134</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712679.1">
        <property type="nucleotide sequence ID" value="XM_018855775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE52"/>
    <dbReference type="STRING" id="869754.A0A1A0HE52"/>
    <dbReference type="GeneID" id="30028751"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000074">
        <property type="entry name" value="T-complex protein 1 subunit delta, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="540" mass="58698" checksum="950BBEC2ECC6D207" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE66</accession>
    <name>A0A1A0HE66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_39390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712782.1">
        <property type="nucleotide sequence ID" value="XM_018857133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE66"/>
    <dbReference type="STRING" id="869754.A0A1A0HE66"/>
    <dbReference type="GeneID" id="30030109"/>
    <dbReference type="OrthoDB" id="4085621at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="207"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="295"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="207"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="375" mass="42484" checksum="76C8139F7A977FAA" modified="2016-10-05" version="1">MARKFLLGAAAVGAGVYLYDQNVQPIFPRDNKGNAIGVSQAAPPKELKDDFHKLGEDTKDLGSQLKKTVNSSVSDVRHKTDDAVSSIKDSEMYNKWSQKLDNYQKDVQTEAEAINNKPLPNRLAAKYHNFVNSLGQTEDEKLKELASSTSARQQEIKKDLAREQQSWSSWWSGKKNDAENKKDELTSAAEKEKNSWFKWGQKEADNAEAKLQKEKSSWVNWADDKSDEAKKTAEDAKKEKNKWVSWGSTKADEAERAAKDAQNDLEAKKNGWVNWGSAKADEAQQRAREGHNDLRATLNDQSQHLSENYEQAKNKALEDYEAARKNLENWSQQAKEKANGVWSRADEEHLKRAGDDFQSAFGNLKRFGEELTSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE82</accession>
    <name>A0A1A0HE82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homoserine O-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_40137</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712915.1">
        <property type="nucleotide sequence ID" value="XM_018857236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE82"/>
    <dbReference type="STRING" id="869754.A0A1A0HE82"/>
    <dbReference type="GeneID" id="30030212"/>
    <dbReference type="OrthoDB" id="191364at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF11">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="60"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="470" mass="52955" checksum="EDD0A82B4D1A34BF" modified="2016-10-05" version="1">MYANYKNVTEEQNRTNPYAAMVPGQTIVEIPRYELECGEVLLDFPVAYKTWGRLNKDHDNVLLICHALTGSSDVQDWWGPLLGTGLTFDPSRFFIICINFLGSPYGSCSPMTIDKSTGKRYGPEFPLVTVKDDIGVQKLILDFLGVEKIACVIGGSMGGMAALEYSSTYHNTGYVKSVIALATAARALAWCISWNETQRQCIFSDPNYRDGYYHETPGLRPDSGLSAARMAALLTYRLRNSFETRFGRKLPSNVQLKRAGAAQDEGIRLPKNKDEESWLLHNDGARSSRQVSSQLLQSLTSSDKPQTYFTAQSYLRYQGDKFVARFDANCYISISRKLDTHDITRGHVDFDENADEDPLVQYLGSLWLPHLIIGITSDGLFTYGEQQLLGAFLPDNTLKTLDSPEGHDAFLLDFEIIDEYCREFLHDRLPEFYDDSGKIEPFENWKDYVVSVENGGNSVFGEAEKNITNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB6</accession>
    <name>A0A1A0HEB6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31347</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712950.1">
        <property type="nucleotide sequence ID" value="XM_018855833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEB6"/>
    <dbReference type="GeneID" id="30028809"/>
    <dbReference type="OrthoDB" id="759142at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291917" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="31"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="212" mass="24160" checksum="D720F0773DC949BA" modified="2016-10-05" version="1">MFANTWVLLSCLLLLAAALQLDVPALDSARPVCIRDFVGKDQLVVISVNTDGYRGDGQKLQLTVIDTLGNQFARKNDINKETRISFTTHESAAIDICFANFLDRGHGGRRQLSREVNLEVESGASARDWNALQTAEKLKPAEVDLRRVHEMTKEIAQELHYLKGREERMRDTNESTNARVQWFSILIILVLLALGAWQIQYLRHYFKVKHII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEC2</accession>
    <name>A0A1A0HEC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">G10 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_151087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BUD31 (G10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712837.1">
        <property type="nucleotide sequence ID" value="XM_018854568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEC2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEC2"/>
    <dbReference type="GeneID" id="30027544"/>
    <dbReference type="OrthoDB" id="277109at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001748">
        <property type="entry name" value="BUD31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411:SF0">
        <property type="entry name" value="PROTEIN BUD31 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411">
        <property type="entry name" value="PROTEIN BUD31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01125">
        <property type="entry name" value="BUD31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00322">
        <property type="entry name" value="G10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="147" mass="16672" checksum="5E1BA1B0469C6C08" modified="2016-10-05" version="1">MPKAPRSKKPPADYEKVEPTLTKLLAKLKEAQSTSLQSSTANKHSSLWPILKLNHQISRYVYSMYYQRKAISKELYDYLIRQKYVDADLIAKWKKQGYENLCCVGCIVVSEKNHGTTCICRVPRADVDGRNQDGCVTCGCRGCFSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEC9</accession>
    <name>A0A1A0HEC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Prephenate dehydrogenase [NADP(+)]</fullName>
            <shortName evidence="9">PRDH</shortName>
            <ecNumber evidence="9">1.3.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_39924</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>prephenate + NADP(+) = 3-(4-hydroxyphenyl)pyruvate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21640"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="ChEBI" id="CHEBI:36242"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8 9">Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NADP(+) route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the prephenate/arogenate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.13" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712753.1">
        <property type="nucleotide sequence ID" value="XM_018857205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEC9"/>
    <dbReference type="STRING" id="869754.A0A1A0HEC9"/>
    <dbReference type="GeneID" id="30030181"/>
    <dbReference type="OrthoDB" id="5399569at2759"/>
    <dbReference type="UniPathway" id="UPA00122">
        <property type="reaction ID" value="UER00962"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3660.10:FF:000004">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000339">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012385">
        <property type="entry name" value="Prephenate_DH_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036510">
        <property type="entry name" value="PDH_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="10">
        <location>
            <begin position="9"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="437" mass="49777" checksum="5D21018808EAE14C" modified="2016-10-05" version="1">MEEEWKKSKTIGIIGLGDMGAMYAERFSSAGWNVIGSDREDKFQETLQKYRGRKFKVLPSGHFVSRSADYIIYSVEAENIRSIVKTYGPSTKVGAIVGGQTSCKNPEIEAFQTYVPEDVDIISLHSLHGPKVDSTGQPLVLINHRSKSENFEFVKCIVSCLKSKVVELSAAEHDRITADTQAVTHAAFLSMGTAWMKINQYPWETPKWIGGIENAKINIALRIFSNKWHVYAGLAITNPSAHGQILQYSRSATELFTLMIEGKKEELKTRIYKARDFVFEHNSGHESLLLNDSILEKFSLSKTPPTYEFQPNSHISLLAIVDSWHKLGIVPYEHMICSTPLFRIFLGVTEYLFRSPKLLDQCIEFAIHDTSFRHDDLEFTFAARRWAEIIARGDYRLYENSFVEAQEFFSPMIPEANAIGNEMIRTILERVRQRGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEF7</accession>
    <name>A0A1A0HEF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial zinc maintenance protein 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_31280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Assembly factor required for Rieske Fe-S protein RIP1 incorporation into the cytochrome b-c1 (CIII) complex. Functions as a chaperone, binding to this subunit within the mitochondrial matrix and stabilizing it prior to its translocation and insertion into the late CIII dimeric intermediate within the mitochondrial inner membrane. Modulates the mitochondrial matrix zinc pool.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with RIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family. MZM1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712866.1">
        <property type="nucleotide sequence ID" value="XM_018855824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEF7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEF7"/>
    <dbReference type="GeneID" id="30028800"/>
    <dbReference type="OrthoDB" id="529194at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20267">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045298">
        <property type="entry name" value="Complex1_LYR_LYRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050435">
        <property type="entry name" value="MZM1/LYRM7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46749:SF1">
        <property type="entry name" value="COMPLEX III ASSEMBLY FACTOR LYRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="144" mass="16060" checksum="DDCC333569A60AAA" modified="2016-10-05" version="1">MPGARIEIAKSQSHQIHHQLKTTYSSAMSRQALTAYRHALRATRVAFNGDNFMLLSARAKVKESILANRNLSDEQETAKSVQHLDEVAAFLAKNIVQGEKQENDRYHLKFHAKTELGSNDTIKENSKANLGSLAGVKVRKCSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEH3</accession>
    <name>A0A1A0HEH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712799.1">
        <property type="nucleotide sequence ID" value="XM_018857212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEH3"/>
    <dbReference type="GeneID" id="30030188"/>
    <dbReference type="OrthoDB" id="4073891at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="321" mass="35895" checksum="EE14CD11924B0CF1" modified="2016-10-05" version="1">MSASPQKLEANLPETPAPRALTTKHKVVACWVLWAATCFTSVICCFPTFTRASLWFPQLRLHLDKTDNGVWVYDVDEVLQVISQFATDLQGVSLADYDPELSFVSDSIYGFDFSSWCRHSPSTVSISCYLGFGMDVITALVTDMGVQVGIAGRMDNPLLFGANLASTFNKIIRDLDLAYHNAQDAPEEALDMARLSIVHQVDQSMKMGRVMLVLNFVHAVLFLATAMAFFVPMAKGVFWRYRRAQIPMFSLLALCETCTLINYLLEALYLRKLNSVFRVHGVILTLGLGSTMLVTEYLAAFVLCILYFVYFHGEFHGESST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEV1</accession>
    <name>A0A1A0HEV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">High affinity nicotinic acid plasma membrane permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_29247</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713135.1">
        <property type="nucleotide sequence ID" value="XM_018855369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEV1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEV1"/>
    <dbReference type="GeneID" id="30028345"/>
    <dbReference type="OrthoDB" id="5298304at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015225">
        <property type="term" value="F:biotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901604">
        <property type="term" value="F:dethiobiotin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905135">
        <property type="term" value="P:biotin import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000399">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000278">
        <property type="entry name" value="Putative MFS transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF33">
        <property type="entry name" value="VITAMIN H TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="465"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="47"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="492" mass="54588" checksum="89467916DFD80356" modified="2016-10-05" version="1">MSTEVEDKQLDQSSASLHIKEESVGSFQDEATKSELDRIYRKLDRRIIPALWCLYFLTSFGSSAYGTTLTMNSSDGHSLGQKLDLTSKDTSTASALYYVGYIIFDVPMNLIMTKVSPQSWLARIVITVGLVYSCYIALESSGALIAVRFISGVVGAGTWPGMSYYISLWYPNERLTRRIGYYFTAAQISAAAAGLLSAAFQKMDGIHGYTGWQWLYLVYGCVTIVVGISLLWWLPDRPYQLSDKTPGKYMTIYNKYFTTAHPLNPEEREIHRKDIQSRYVLIKWTWKDVSAIFLDLRLWPIIMMYFGVVGTGFGLAVFGSTIIAANNPSLSSINVSLLYAPIWLFDLAGILLITPFADRYKKHRAFFFSGACLIIITGMFVTTFANGSWNRYGGLLIAGFGLGPTVPICMSWSAEIFGPVYGDSGIAVSSALVSGLGNLGSVTATYALYRGWPSDAARLYRNSNMVLVAMLGISIIFSFVCHFIKDRVGRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEY6</accession>
    <name>A0A1A0HEY6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713165.1">
        <property type="nucleotide sequence ID" value="XM_018855375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEY6"/>
    <dbReference type="GeneID" id="30028351"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="134" mass="14889" checksum="1AD0A3C8E88B82C4" modified="2016-10-05" version="1">MTCESIDGEGLVLIQDFTQNLLPESSRDAGEDPHLGSKQVGTEVEMSDILVPDISDDGWKVQGRKRRSSRKRPEPSVIMATTRGSDLLDQKGFLGSNPSMKQPSSNLNPFLVLAEISEEDFQQATLRRYLPLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF45</accession>
    <name>A0A1A0HF45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713235.1">
        <property type="nucleotide sequence ID" value="XM_018855384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF45"/>
    <dbReference type="GeneID" id="30028360"/>
    <dbReference type="OrthoDB" id="10557666at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="213" mass="24053" checksum="6731D20817173337" modified="2016-10-05" version="1">MGLDSHTKGHGHNSSEDSLSIYSPIRHDQDSGIHPQTRDSVTSRTSMVPCTTRSFEAETNEPHVILAGIETSCSFELFKAPSARSNIFSHEDSMILKYLITLHRSLEDPALYFKWGVWRNGGPDEGLRLEDILETEVQLVTWNKQIKKDCSGEAAYLRLGLHPDRICGKVAFQISLRDARNFFQEVASVVLRELLSLPMAIYSTYAFAAVYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF81</accession>
    <name>A0A1A0HF81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_155380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713039.1">
        <property type="nucleotide sequence ID" value="XM_018854606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF81"/>
    <dbReference type="GeneID" id="30027582"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="17618" checksum="B090FC042178E8E4" modified="2016-10-05" version="1">MMVEADLRFRPTRLDYYLKHKSDHQKQALRIHKCSGAELKGIDKLECLVPSFLGLAIWYRCAHQSLTQPNNVSIKAYTLSKTRCKLLLRCEAKLFWCEKIGSVRIWPWSSGKHTGNKRLTFTQLSRKGPETPDSWLERFFLKPQPAFMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY6</accession>
    <name>A0A1A0HFY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA replication regulator Sld3 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713249.1">
        <property type="nucleotide sequence ID" value="XM_018856717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY6"/>
    <dbReference type="GeneID" id="30029693"/>
    <dbReference type="OrthoDB" id="5395343at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2130">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013948">
        <property type="entry name" value="DNA_replication_reg_Sld3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042511">
        <property type="entry name" value="Sld3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28067:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08639">
        <property type="entry name" value="Sld3_STD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication regulator Sld3 C-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="386" mass="42428" checksum="A53E11CF8B50EDCE" modified="2016-10-05" version="1" fragment="single">MCPFEAENQALFVSKHYEQTTKDELLEHLVLELKIREAQIQIVLLLEIILCMAVDEADFLDTGRRQQAQAAASKRKPALIRRKKKKVIATFLGVGVTEPEGVSGTVMLSPKQDHMSPLSLYTSLVGLIDQIGLWDSLLEHGRTDKDENTQGFLAFVVVPFFGKGLPKTVLFIIKQIKGLRPNILRTRWTKSASSLEPPGLPQNQPSDPQSKFPKISMSTDSIPTLPRASTTTDADSIKPAFSLKRSKSNLGTRNLKRRQVDMSVAKNEPADTEPGKSNLFLFTDARRVKSGASLAQSSQSFTNVRQVEATPAKPAAPGASRNAPRVFATPQVLATPSNQRTVDGGYIVKETPQGRQEKKPSVHERLSQVARESPVKSMGIVSSPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFZ4</accession>
    <name>A0A1A0HFZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29210</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713298.1">
        <property type="nucleotide sequence ID" value="XM_018855368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFZ4"/>
    <dbReference type="GeneID" id="30028344"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="103"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="453" mass="49880" checksum="099C4EF78B62E52C" modified="2016-10-05" version="1" fragment="single">VTQLPTPVMPCGAFPAVLPVKIDVAKRSTTPVAAGRARIVSAFDPEPLLVPVLPTTQPPTRHISNPGAPVAGVPRAVPLPAQEPEQPPLQGRVLHAPASGRTYVCEDVVGTGAFSTVARARDAADPALEVAVKIVLFPRDGSTSVSSFRAFIVRELGLLAHLHHPCIVTLLDYSVTPLITPLQIRASYDNGGSPPVAEDLSQTADAQQLFFLGYCRGGNLFSWLLAHYHGLSRTLRFWQLVARVVAEVVVAVVHMHRQRIIHRDIKLENVLLNERFVCTGPAPDPRRLSRPVATITDFGLSKRLALHDQLLSTKCGSQDYVSPELLMGLHYSGKLLDAWALGVLVYSIIENRLPFDMPSPDLLQQAGVSPSAPRRRRSRHSPAHRIAMIDWEWYRAVAIRHDNDMDPEARKIVGDLQALVDTFLVRKEKRPTVEEALETDAFAWIKECVPSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG18</accession>
    <name>A0A1A0HG18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alkyl transferase</fullName>
            <ecNumber evidence="4">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713427.1">
        <property type="nucleotide sequence ID" value="XM_018856904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG18"/>
    <dbReference type="STRING" id="869754.A0A1A0HG18"/>
    <dbReference type="GeneID" id="30029880"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="320" mass="36248" checksum="E2A9F223B0478F6A" modified="2016-10-05" version="1">MPEWISTFPGYSQLMRKIKCTIGGVCKTGPVPRHVGVIMDGNRRYAKTNKIEMKEGHNLGFDSMASILELLYESGVACATVYAFSIENFKRLRYEVEWLMNLAKSKLYQMSQHGELCDQYGIRIKILGNVSLLPADVQKILQDTQKMTAHNTRAVLNVCFPYTSRDEIAHSIRGVVKASMEDPEMIIDEHTMENYLYTADAPPLDLLIRTSGTSRLSDFLLWQSVPSTCAIVFSDRLWPEFLPWDMTKILLSWSFNMYWYGHGNGQTMSKIVLHSSALPSLKNHDASLFADSDVSTAYERYTTESPVDATERLSLATGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG54</accession>
    <name>A0A1A0HG54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">COX5B-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_76215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713625.1">
        <property type="nucleotide sequence ID" value="XM_018858496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG54"/>
    <dbReference type="STRING" id="869754.A0A1A0HG54"/>
    <dbReference type="GeneID" id="30031472"/>
    <dbReference type="OrthoDB" id="10249250at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00924">
        <property type="entry name" value="Cyt_c_Oxidase_Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.11.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit Vb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002124">
        <property type="entry name" value="Cyt_c_oxidase_su5b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036972">
        <property type="entry name" value="Cyt_c_oxidase_su5b_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10122">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 5B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01215">
        <property type="entry name" value="COX5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51359">
        <property type="entry name" value="COX5B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602124-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="314" mass="34290" checksum="C4C34452440199D8" modified="2016-10-05" version="1">MELCGGSFKNVAKYRRQAKKTSALRPAVYRANVYFLYYNGLLSSILRRDLLVLSESLSLRRSSDSSVDSGSDSHDSGVDGTRNTVVQLVVQLWQSVLLVHRSLGQVSDGSSLNHVSDGDSLDGLVLWHTSGTVQTSDWLDVASTLLVSSVGSSLLWHVAGMLSRTIARVARQSRTLSSSRIVFSATKLESKTATKLADVTGPDSSLIGAGATPGTIPTDIDQATGLERFEILGKMEGVDVFDMENPIFEGKGTMKDPYMVPTYIGYRYVGCRGHDTQDHKPYWMKVEEGNPGRCWQCGSVYAAKYVGEPGHHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG74</accession>
    <name>A0A1A0HG74_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_228278</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713471.1">
        <property type="nucleotide sequence ID" value="XM_018855200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG74"/>
    <dbReference type="GeneID" id="30028176"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="871" mass="98880" checksum="F151DFFAE2D5BE0D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGA9</accession>
    <name>A0A1A0HGA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_101876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713680.1">
        <property type="nucleotide sequence ID" value="XM_018853705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGA9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGA9"/>
    <dbReference type="GeneID" id="30026681"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="11839" checksum="8453746719140CE5" modified="2016-10-05" version="1">MKPVLCFCRKETVAGYFARELLLRRKKVSSRSKRGSDASLCTMRGNYPPLLMNGTFRNSSSAKNYSARECEDAFNREEFVMILKTVEMITISCDFVKVKVYCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGB2</accession>
    <name>A0A1A0HGB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_228290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713511.1">
        <property type="nucleotide sequence ID" value="XM_018855201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGB2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGB2"/>
    <dbReference type="GeneID" id="30028177"/>
    <dbReference type="OrthoDB" id="4329339at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043457">
        <property type="term" value="P:regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044792">
        <property type="entry name" value="TAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188:SF1">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="111" mass="13002" checksum="46409AB2AD755771" modified="2016-10-05" version="1">MCFSSSRHCTCSLSVSHQYPALDGIYHPLRAAFPNNSTLWWSKKMDSSRGYHPPWRHFPMDLRMFELAETTFKLQFPEGFKFEPLLLHSPLLRQSLLVSFPPLIDTLKFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGE8</accession>
    <name>A0A1A0HGE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TAP42-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_76078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713409.1">
        <property type="nucleotide sequence ID" value="XM_018858474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGE8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGE8"/>
    <dbReference type="GeneID" id="30031450"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="384" mass="43766" checksum="E79EA6C67EFF1359" modified="2016-10-05" version="1">MLSNHSDLTVRQRLNLIVKSCAFIENSTERQDSASFQERVFDVLKQLSMLQSITLSLSLFSSNEQLNEINTSYLPFAALLYYEAILYPKLFIDPKENIDSDSVDILLFKPRNLKTAKVKNLEYLTLVDSFRDILSPEQLSILNSFKKSYNPSNEDILAATGDPALRRASKIASYKLEKDLLGKVVALDEYYANFGDRDGADEDLLQKMDEDTVRSLYENQLRLFSVNAFKNLELIAMELQVLESRDSQQDIRATEDIRVQKMPLVDRFEYTSRLESDPTKQKDIADLISKLGKILQPFVITGSREKLRSSVFGTGQVLPSMSIEEYLDYELANGKMASNDAKDSQMGDSDDNTDDSDEEIRKREWDDWKDENPKGSGNMKANMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH05</accession>
    <name>A0A1A0HH05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calreticulin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_9462</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the calreticulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713643.1">
        <property type="nucleotide sequence ID" value="XM_018859396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH05"/>
    <dbReference type="STRING" id="869754.A0A1A0HH05"/>
    <dbReference type="GeneID" id="30032371"/>
    <dbReference type="OrthoDB" id="1938156at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.250.10:FF:000001">
        <property type="entry name" value="Calnexin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.250.10">
        <property type="entry name" value="Calreticulin/calnexin, P domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001580">
        <property type="entry name" value="Calret/calnex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018124">
        <property type="entry name" value="Calret/calnex_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009033">
        <property type="entry name" value="Calreticulin/calnexin_P_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073:SF1">
        <property type="entry name" value="CALNEXIN 14D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11073">
        <property type="entry name" value="CALRETICULIN AND CALNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00262">
        <property type="entry name" value="Calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00626">
        <property type="entry name" value="CALRETICULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63887">
        <property type="entry name" value="P-domain of calnexin/calreticulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00803">
        <property type="entry name" value="CALRETICULIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00804">
        <property type="entry name" value="CALRETICULIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="492"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="232"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="279"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="127"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR601580-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="573" mass="64248" checksum="3E7CE7644FCF7FDA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH35</accession>
    <name>A0A1A0HH35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33962</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I LYR family. SDHAF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713639.1">
        <property type="nucleotide sequence ID" value="XM_018856429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH35"/>
    <dbReference type="STRING" id="869754.A0A1A0HH35"/>
    <dbReference type="GeneID" id="30029405"/>
    <dbReference type="OrthoDB" id="273010at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20268">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045295">
        <property type="entry name" value="Complex1_LYR_SDHAF1_LYRM8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675">
        <property type="entry name" value="LYR MOTIF-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13675:SF1">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="5">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="78" mass="9646" checksum="1239C4E10C41D5FD" modified="2016-10-05" version="1">MPRLSGLQREVLKLYRECTRSVNTKPREFQDNWRTYVRLEFAKYHHLPKKSFSVIEHLLRVGRRRYEMYLDPNIKDVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH48</accession>
    <name>A0A1A0HH48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_29724</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713683.1">
        <property type="nucleotide sequence ID" value="XM_018855466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH48"/>
    <dbReference type="STRING" id="869754.A0A1A0HH48"/>
    <dbReference type="GeneID" id="30028442"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043573">
        <property type="entry name" value="Fig4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45738:SF5">
        <property type="entry name" value="POLYPHOSPHOINOSITIDE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="5">
        <location>
            <begin position="214"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="744"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="930" mass="106143" checksum="392CEE975897DB28" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHE8</accession>
    <name>A0A1A0HHE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_228448</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713906.1">
        <property type="nucleotide sequence ID" value="XM_018855207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHE8"/>
    <dbReference type="GeneID" id="30028183"/>
    <dbReference type="OrthoDB" id="4046837at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="487" mass="54870" checksum="F697C41A355864AE" modified="2016-10-05" version="1">MLRSLPGCRPYRGIRWNSGLAALTGDLRLGKHVRSSDVENALTNRKMTTSDIEHLHEILRLNIEEDIANEVLYHGLPHDFSLYFTASRRPQGLKLNNRALEPLIKHNPGRVFSLTDLLAKHGESVSDAVRIAVVDKLLCGENSEADDVDFSPSDTSISKAIALLNEVSNLSFSEESLSKLIEVLREKGAIAALSILKIDGLYTWLYTTKLATEKDPHVFLALSQLIFTHDRTLLRKETLGKILSYGSLMEKPDSTSALQNFVLSERPLGIKEYTDAVLEYVEEQKLDFDKKDPEALFLRMQLMETYGINKDDVDSALRKFHEYQTHEKFGIDLVQANIVKVFCYQSFKTKNETFMKIAETLVSPEGLAVSTVAQLILAKSRFSSEKSLEIYNEYINLVSKKVNPATGRSPTGVLTESLMVASLYDNDREFAQLLYEKAIVNGIISNEHEVASIKKVFKTYGDSYVEDDWKVAQPVFAEFVLECIKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHH8</accession>
    <name>A0A1A0HHH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713942.1">
        <property type="nucleotide sequence ID" value="XM_018855513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHH8"/>
    <dbReference type="GeneID" id="30028489"/>
    <dbReference type="OrthoDB" id="4081076at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291844" evidence="1">
        <location>
            <begin position="22"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="142" mass="16410" checksum="AFE12AB2C649E873" modified="2016-10-05" version="1">MYELSFTLMGILILVQEITRSLPVYTFLGTLRSNQEINSETNYNLICAYEAHQVRPVMEISTITTKEKVRMWTVDPAKKPFAEKSEWEELDRELVLEDSLLMSCVSEKHVANICSWTNEETNANLNAKTIKKIANKHPQKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHM0</accession>
    <name>A0A1A0HHM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Postreplication repair E3 ubiquitin-protein ligase RAD18</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Postreplication repair E3 ubiquitin-protein ligase rad18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 18">RING-type E3 ubiquitin transferase RAD18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">METBIDRAFT_110397</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RAD18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714140.1">
        <property type="nucleotide sequence ID" value="XM_018853904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHM0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHM0"/>
    <dbReference type="GeneID" id="30026880"/>
    <dbReference type="OrthoDB" id="9049620at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097505">
        <property type="term" value="C:Rad6-Rad18 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000172">
        <property type="entry name" value="E3 ubiquitin-protein ligase RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039577">
        <property type="entry name" value="Rad18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006642">
        <property type="entry name" value="Rad18_UBZ4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14134:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RAD18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00734">
        <property type="entry name" value="ZnF_Rad18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51908">
        <property type="entry name" value="ZF_UBZ4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14 20" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="22">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ4-type" evidence="23">
        <location>
            <begin position="148"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="348"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="367"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OBA23659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="419" mass="47854" checksum="2E38622932293041" modified="2016-10-05" version="1">MSEFEPSDWKLTRIPKLSQLDSLQRCLICKDFLKAPVITSCNHTFCSQCIRQHLMIVSQCPLCKAEQFESNLKRVILLEEIVLCFQALRDDLISLLQEEESSTNDQQDNMKTIKNQQVHLDVIEIPDHSEGLDSNSTLAQGTILDAGYVHCPVCGEVMKEEHVQGSHLDYCLNGKPDPKLMLKTHSVKRKTNDVVLFFQGRKRQKNAANNVDHANFYFNKGDQHHHNTKRIPKVDFSSLSTAKVKEKLMALKLSTLGSRTELEWRYNHYYLLNQSNLDSNHPLTEMELRQKLKQTEISHLMTPLTSASNTIYGDSLSRKSLADKDFPIKAWLDIYKDEFKQLVKQARKSRKKQALTKNMKSPDHATRASTNDIASSRSATNPLTNEENKNVHLGDQASHREKDPADDFDFSTSALFAPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHP9</accession>
    <name>A0A1A0HHP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Brl1/Brr6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_19906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714012.1">
        <property type="nucleotide sequence ID" value="XM_018854908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHP9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHP9"/>
    <dbReference type="GeneID" id="30027884"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="2">
        <location>
            <begin position="8"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="175" mass="19948" checksum="9166B68432C574A9" modified="2016-10-05" version="1" fragment="single">DPRIPYVLLLYLQLFFNAVIVSVVIYLLYTVVRTVRQDVRHKIEMYTADAIQEISLCSRQYYRNRCSNDGTNIRAPALEETCMALEKCMNRDPQQIGKSQITAETFADILNGFMNPISWKLTVLFTGLIVGGVFIINVAFGTYRSRSGMEDFSKNTNATNSVVRSRKEMVLCPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI01</accession>
    <name>A0A1A0HI01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Small nuclear ribonucleoprotein E</fullName>
            <shortName evidence="11">snRNP-E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Sm protein E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_34148</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in pre-mRNA splicing. Binds and is required for the stability of snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site. Involved in cap modification.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714270.1">
        <property type="nucleotide sequence ID" value="XM_018856447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI01"/>
    <dbReference type="STRING" id="869754.A0A1A0HI01"/>
    <dbReference type="GeneID" id="30029423"/>
    <dbReference type="OrthoDB" id="25620at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990935">
        <property type="term" value="F:splicing factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01718">
        <property type="entry name" value="Sm_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027078">
        <property type="entry name" value="snRNP-E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11193">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="12">
        <location>
            <begin position="14"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="88" mass="9853" checksum="28237F7B9E13E835" modified="2016-10-05" version="1">MSTVKSKQAVLPPINLIFKYLQQQSLVTIWLYEQTQLRIQGKVRGFDEFMNIVIDDAVEISTASGAKETLGRILLKGDNITLISSLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI26</accession>
    <name>A0A1A0HI26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-exchange factor SEC12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_28401</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Guanine nucleotide-exchange factor (GEF) required for the formation or budding of transport vesicles from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Golgi apparatus membrane</location>
            <topology evidence="11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat SEC12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714141.1">
        <property type="nucleotide sequence ID" value="XM_018855354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI26"/>
    <dbReference type="STRING" id="869754.A0A1A0HI26"/>
    <dbReference type="GeneID" id="30028330"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045260">
        <property type="entry name" value="Sec12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23284:SF0">
        <property type="entry name" value="PROLACTIN REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <keyword evidence="2 10 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="325"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="41608" checksum="A218E026CD26FA76" modified="2016-10-05" version="1" fragment="single">MAAKDFAEIDTGFPVYGLRFINNHTLLACGGGGEGNNGIPNKVTAVRCSFTVSDKSRRLQKFREITLPAQEDSPMCIEFCRSSVGDNSRFQIFVGCNQSTELIRSMNINNNLRKYNYTEDEHLQFLDAVQFDENLLVESIGEYPKIVHLSTDASIGGMMTSRIPSEIFIFNPDTLELTLRFKPPAASEIKDFHFNQDDSGKTFTYVTASFIETISTHSGNSISSSANADKKTAKILGKYFFSKVRYVNGPKVIIAAASRNGKGAAVLEYNTETRKVLREVIISNKIKGIVAIDVSKSSGMFAVAGNDFSVSLLRVTDFKMIKTYTKLHKFAVTCLSFSPDGKKLATGSASNTVNVLLVKPSSGGFFSFIFSLFRFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI82</accession>
    <name>A0A1A0HI82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">t-SNARE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_76544</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714029.1">
        <property type="nucleotide sequence ID" value="XM_018858549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI82"/>
    <dbReference type="STRING" id="869754.A0A1A0HI82"/>
    <dbReference type="GeneID" id="30031525"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.70:FF:000008">
        <property type="entry name" value="Syntaxin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF307">
        <property type="entry name" value="PROTEIN SSO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="199"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="298" mass="34354" checksum="4581697557650007" modified="2016-10-05" version="1">MSNPYYNGGANGAGANHNTNSYEMNAFKYEEADDFVEFMNEIQDINLQLDNYSNLVDLIANKQKNHLQDLDLNDDGDEGEYMSKQIDLLVLEASSLQSDLKSRIKNVQTQAAQLRNPQKIDQAETSRNKFLDLIQRYRLTESNNREQTRVQAERQYRIVKPDATPEEIQAVVEDGSGNQQIFQQALMQLNRRGEARTVLNEVQVRHRELLKLEKTMAELTQLFHDMEELVVEQDQPIQQIEQQIDTAQHDIEQGVGHTNKAVVLAKKARKKKIWCFFICIIILCILALILGLYFGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIA0</accession>
    <name>A0A1A0HIA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Tom7-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_34760</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714375.1">
        <property type="nucleotide sequence ID" value="XM_018856522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIA0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIA0"/>
    <dbReference type="GeneID" id="30029498"/>
    <dbReference type="OrthoDB" id="284357at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012621">
        <property type="entry name" value="Tom7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34944">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34944:SF2">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08038">
        <property type="entry name" value="Tom7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6788" checksum="88ED8F804E6D7AD8" modified="2016-10-05" version="1">MATTYQLTLSDESKERIMKVLGYSRTIAHYGFIPFILYLGWKSTPSKPSLFSLLSPFPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIA4</accession>
    <name>A0A1A0HIA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rho GTPase activation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_24494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714380.1">
        <property type="nucleotide sequence ID" value="XM_018855280.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HIA4"/>
    <dbReference type="GeneID" id="30028256"/>
    <dbReference type="OrthoDB" id="79452at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037769">
        <property type="entry name" value="Abr/Bcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182">
        <property type="entry name" value="BREAKPOINT CLUSTER REGION PROTEIN BCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23182:SF1">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 1A, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="280"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="5">
        <location>
            <begin position="804"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="1443"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="6">
        <location>
            <begin position="1499"/>
            <end position="1691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1345"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1692" mass="193959" checksum="9CAAE51236D2A7EB" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIJ5</accession>
    <name>A0A1A0HIJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_76889</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714455.1">
        <property type="nucleotide sequence ID" value="XM_018858604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIJ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIJ5"/>
    <dbReference type="GeneID" id="30031580"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd23813">
        <property type="entry name" value="UBCc_UBE2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000020">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="3"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="16905" checksum="58889430E30E5A06" modified="2016-10-05" version="1">MAALPKRIIKETERLVLDPVPGINAVPSEENLRYFEVTIDGPSSSPYADGQFQLELFLPDDYPMCAPKVRFLTKIYHPNIDKLGRICLDVLKNNWSPALQIRTILLSIQALLGAPNPDDPLANDVAQEWKADEAKAIAVAKEWTEMYAKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIP0</accession>
    <name>A0A1A0HIP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_36154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714352.1">
        <property type="nucleotide sequence ID" value="XM_018856696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIP0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIP0"/>
    <dbReference type="GeneID" id="30029672"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF28">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="277"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="386"/>
            <end position="393"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="644" mass="72505" checksum="2E529F8BBB249390" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIP6</accession>
    <name>A0A1A0HIP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C4-dicarboxylate transporter/malic acid transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76941</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tellurite-resistance/dicarboxylate transporter (TDT) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714510.1">
        <property type="nucleotide sequence ID" value="XM_018858610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIP6"/>
    <dbReference type="GeneID" id="30031586"/>
    <dbReference type="OrthoDB" id="1099at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000319">
        <property type="term" value="F:sulfite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="entry name" value="Voltage-dependent anion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051629">
        <property type="entry name" value="Sulfite_efflux_TDT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31686:SF1">
        <property type="entry name" value="SULFITE EFFLUX PUMP SSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="52236" checksum="FDCFD30B118DA6B1" modified="2016-10-05" version="1">MMCGPSASSGETVGSKPGLAEVPRISGTNSNSNDLVETVQTHPASWLQSVSAELRARFIDGFSPAYFASVMGTGMSANLLYNFPYPARWLQVCGIIMALVGLVLFVGLCGFFAVALYCRPRLFSQIHRDPVVAPAMGCFVMGYTTLFTFLHNVLGASWIVGVWVLWWVVVFGSFYTGIVTFYFSLMAKHRNSKNRLEPTSLGMHYLLPVVTLTVAASMGGIITADLPTTNDKIVTMTVSLIMWAIAMLLASIIVTINFWRMLVHKIPHSGQVLAMFLPIGFVGQGAFAILYFGQNCTLLLLEHHDTVAASLYVSFLSDTAQNNGVALTHLPLLMSTAIMVTCSIVAFMLIAFGFLLTVFAYMSLVSKMSPFAIKRNHAFLYQPTSRLPIKRFFAGLLKFQRGFWSMTFPLGTMSLAINHFYSLYNGFVAFRVVATIYAMLVLITTLGCLVGVVYKGLMIFIRARATAESKNDEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIU9</accession>
    <name>A0A1A0HIU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_30436</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714565.1">
        <property type="nucleotide sequence ID" value="XM_018855635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIU9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIU9"/>
    <dbReference type="GeneID" id="30028611"/>
    <dbReference type="OrthoDB" id="260807at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101:SF80">
        <property type="entry name" value="PHOSPHATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14565" checksum="9C1DA6D24797CF41" modified="2016-10-05" version="1">MECLGAVLVENRVSDAVRKKIIKTGTLEDDPAVLMLAMATALVGSPIWLLTATVIGMPVSTTHSIIGAVIGVDIVSKGADYVIWGWGGFAQIAASWFIAPGIAGGFASFLFCKYFILETRERGSRTLWLLGPFWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIZ8</accession>
    <name>A0A1A0HIZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ADP/ATP translocase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ADP,ATP carrier protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_37689</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the exchange of ADP and ATP across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ADP(in) + ATP(out) = ADP(out) + ATP(in)</text>
            <dbReference type="Rhea" id="RHEA:34999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:35000"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714457.1">
        <property type="nucleotide sequence ID" value="XM_018856906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIZ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIZ8"/>
    <dbReference type="GeneID" id="30029882"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140021">
        <property type="term" value="P:mitochondrial ADP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990544">
        <property type="term" value="P:mitochondrial ATP transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000001">
        <property type="entry name" value="ADP,ATP carrier protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002113">
        <property type="entry name" value="ADT_euk_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45635:SF14">
        <property type="entry name" value="ADP_ATP TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00927">
        <property type="entry name" value="ADPTRNSLCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0050">Antiport</keyword>
    <keyword evidence="11 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="108"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="208"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU368008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA23976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="32568" checksum="F9BFCD017EE5F556" modified="2016-10-05" version="1">MSDNSFLIDFMMGGVSAAVSKTAAAPIERVKLLIQNQDEMMKQGRLAKKYDGIVECFKRTAAEEGVSSFWRGNTANVVRYFPTQALNFAFKDKFKKMFGFKKEESYAKWFAGNLASGGMAGATSLAFVYSLDYARTRLANDAKSTKGGEGRQFNGLLDVYKKTLASDGIAGLYRGFGPSVIGIVVYRGLYFGLYDSLKPVVLVGPLEGSFLASFLLGWTVTTGASTASYPLDTVRRRMMMTSGQAVKYDGAFDCFKKVVAAEGIPSLFKGCGANILRGVAGAGVISMYDQLQVILFGKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ43</accession>
    <name>A0A1A0HJ43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Kontroller of growth</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_35216</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714516.1">
        <property type="nucleotide sequence ID" value="XM_018856582.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HJ43"/>
    <dbReference type="GeneID" id="30029558"/>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031139">
        <property type="term" value="P:positive regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029347">
        <property type="entry name" value="Raptor_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14538">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM01302">
        <property type="entry name" value="Raptor_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Raptor N-terminal CASPase-like" evidence="6">
        <location>
            <begin position="64"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1294"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="414"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1406" mass="158918" checksum="762189BB97843BED" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ71</accession>
    <name>A0A1A0HJ71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_10096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714369.1">
        <property type="nucleotide sequence ID" value="XM_018853683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ71"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ71"/>
    <dbReference type="GeneID" id="30026659"/>
    <dbReference type="OrthoDB" id="21467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12307">
        <property type="entry name" value="RRM_NIFK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46754">
        <property type="entry name" value="MKI67 FHA DOMAIN-INTERACTING NUCLEOLAR PHOSPHOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="86"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="229" mass="26246" checksum="BC02D73D53BBF3A7" modified="2016-10-05" version="1">MAKKNSNVPKALPVEAVQKSEELSLENTPDSASSSEGDSEDFSSDEDIDVQGLEEELTTKKSAHTVKLTKESKKSEGKIDAAKQRAVIYVGRLPKLFEEKEMKKYFSQFGDITRVRVSRNKVTGASRHYAFVEFKDLHAAQVAAETMNNYLLVGHLLKVHLVENPKENLFSKNMKSSFREFDWRAKEYEQQNAPKPLETWKELQKKYEESKKQKFSELKKLGFDYALEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJK8</accession>
    <name>A0A1A0HJK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_30422</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714553.1">
        <property type="nucleotide sequence ID" value="XM_018855633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJK8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJK8"/>
    <dbReference type="GeneID" id="30028609"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="95"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="17"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="416" mass="45738" checksum="A915FAACE49A1ED1" modified="2016-10-05" version="1">MGPPVEGMTNDDKNNKNDTGSNTNGNTNDTDTTSKNNNTNNTNNTDTTNRKNDTNSTDTSSAAFLQPPIAAPSNRPPCPRANTRTSQSGPRVWSKVARTPIFDGANGVIYKALDASRSVFVVVKTVKVQPWHTPDVYRRSVLREYDNLARCQACRHVATVHDVCSSDASPELSLVVQYYPHGDLLDFLGSLRAKRVAMPPSLQDAVFKQIVRAVDFLHRHDVVHRDLKPENFLIDAQGSLRLNDFGCSLDLQRLGEQLPLNGPSCGTPSFKSPELFGLEAAARAGTPADPAFDFRAVDVWALGIVCFHVYLMAAPWPHANVAADDRAKALDLYLKNFPLDDAQVRRLADRLNDTRCSTQLNPALLLFKKMHYDARLELLAMLHPVPAKRPTTGALLASSWLSQAYAKPGDILALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJZ4</accession>
    <name>A0A1A0HJZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_77284</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714980.1">
        <property type="nucleotide sequence ID" value="XM_018858672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJZ4"/>
    <dbReference type="GeneID" id="30031648"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000003">
        <property type="entry name" value="C6 transcription factor (Leu3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF6">
        <property type="entry name" value="TRANSCRIPTION FACTOR SEF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="901" mass="100516" checksum="3A541C13C7D45FD2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKB3</accession>
    <name>A0A1A0HKB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30629</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714808.1">
        <property type="nucleotide sequence ID" value="XM_018855670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKB3"/>
    <dbReference type="GeneID" id="30028646"/>
    <dbReference type="OrthoDB" id="10253125at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="9">
        <location>
            <begin position="72"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="250" mass="27012" checksum="4DB7B7C5D79A0E44" modified="2016-10-05" version="1">MSAPAQAAPKREFGRKPRGPKRGRRDDEKAWVPVTKLGRLVAAGKISSIEEIYLHSLPVKEYQVIDALLPELKDEVMKIRSVQKQTRAGQRTRMKAVVIIGDSNGHVGLGIKTAKEVASAIKAAIIIAKLSIIPIRRGYWGSNLGEPHSLPCKVTGKCGSVSVRLIPAARGKGIIASPVVKKLMQLAGVEDVYTSSSGSTRTTENTLKAAFIAIGNTYGFLTPNLWDVKPLGASPLDTYAEEAKGSKRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKI6</accession>
    <name>A0A1A0HKI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_36805</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in nucleolar integrity and required for processing of the pre-rRNA for the 60S ribosome subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CGR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715021.1">
        <property type="nucleotide sequence ID" value="XM_018856777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKI6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKI6"/>
    <dbReference type="GeneID" id="30029753"/>
    <dbReference type="OrthoDB" id="3942380at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005579">
        <property type="entry name" value="Cgr1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03879">
        <property type="entry name" value="Cgr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="132" mass="16079" checksum="921B92EA7661C69C" modified="2016-10-05" version="1">MSATSEKQFKDIPKRYIDPLVKKDEGEGTRVNGKDWKIKKDAFRLKSAGVRKMNTWKLREEKKLQNEQFKLRINSLKQEKADEKNKKIEQVKQRRQAIEEKERYERIAQKMHAKKVDRLRKKEKRNKLLRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKJ9</accession>
    <name>A0A1A0HKJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutathione synthetase</fullName>
            <shortName evidence="9">GSH-S</shortName>
            <ecNumber evidence="9">6.3.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_10484</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>gamma-L-glutamyl-L-cysteine + glycine + ATP = glutathione + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9 11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic GSH synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714814.1">
        <property type="nucleotide sequence ID" value="XM_018853778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKJ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKJ9"/>
    <dbReference type="GeneID" id="30026754"/>
    <dbReference type="OrthoDB" id="2020073at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.50:FF:000002">
        <property type="entry name" value="Glutathione synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1760">
        <property type="entry name" value="Glutathione synthase, substrate-binding domain superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1080.10">
        <property type="entry name" value="Glutathione Synthetase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005615">
        <property type="entry name" value="Glutathione_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014042">
        <property type="entry name" value="Glutathione_synthase_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014709">
        <property type="entry name" value="Glutathione_synthase_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014049">
        <property type="entry name" value="Glutathione_synthase_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037013">
        <property type="entry name" value="GSH-S_sub-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004887">
        <property type="entry name" value="GSH_synth_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01986">
        <property type="entry name" value="glut_syn_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11130:SF0">
        <property type="entry name" value="GLUTATHIONE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03917">
        <property type="entry name" value="GSH_synth_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03199">
        <property type="entry name" value="GSH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001558">
        <property type="entry name" value="GSHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutathione synthase substrate-binding" evidence="13">
        <location>
            <begin position="214"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="147"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="279"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="413"/>
            <end position="416"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="477"/>
            <end position="478"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001558-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="490" mass="55073" checksum="BF01DDD1EFCBA9BA" modified="2016-10-05" version="1">MTHKEFPALSADNKDLLVEELLQWCLANGLVMYPPNFHKFSANNAPITLFPTPFPKDMFERAVAVQKLFNSLYASVVARQKPWLLEVVSKLAVFDAGFTGKLYETYTKAVEIGGGSVVQPLTLGVFRSDYMYDELTHAIKQIEFNTVSVSFGGLSSKVGHAHTYLNKRGSYDDKYSQKFYQDEELPISKSISEIALGLAKGNFFYNDHKENTSTVILFVVQNGERNCFDQRHIEYELLEKHGLKSYRLSLEQIQEHTTINQGNLYIKLTMDEVSVVYYRSGYAPLDYESNPEATWDARLHLESTKAIKCPSLLTQLSGAKKIQQVLTVPEVVRSFLPDISTKELDNLLSTFVKIYPLDDSAEGTAAKKLAFESPEKFVLKPQREGGGNNVYKQNIPGFLKSLNEKDWGAYILMELINPPTHGNTILRNDELYQEDIISELGIFGNVVFNENTGDVLLNENAGFLLRSKFSSSDEGGVAAGFGCVDNVYLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GQR4</accession>
    <name>A0A1A0GQR4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidate cytidylyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_192353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000039">
        <property type="protein sequence ID" value="OBA14050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709217.1">
        <property type="nucleotide sequence ID" value="XM_018854851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GQR4"/>
    <dbReference type="STRING" id="869754.A0A1A0GQR4"/>
    <dbReference type="GeneID" id="30027827"/>
    <dbReference type="OrthoDB" id="5673at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303:SF1">
        <property type="entry name" value="CTP-DEPENDENT DIACYLGLYCEROL KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA14050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14463" checksum="B654628A155819B8" modified="2016-10-05" version="1" fragment="single">SLVLSFAPKDVAVVSILLLSWADTAASTVGRAWGKYTPKVAQGKSLAGCLASFFVGVLVCYFYYGYLVPKYQVDSIGDNYYSESSSHLSFPYYAFATGLVTSFSEAVNVAGLDDNFTIPVISSVVLSGLIYANHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ57</accession>
    <name>A0A1A0GZ57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33875</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709274.1">
        <property type="nucleotide sequence ID" value="XM_018856414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ57"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ57"/>
    <dbReference type="GeneID" id="30029390"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="4"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA16975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="257" mass="28286" checksum="A6C8D76415EBCC17" modified="2016-10-05" version="1">MKLRMSGAEFRNTGKVDFTSFSRFGESSYVIFARKGFWNEGEIAFGICTNQTQSIPFVVASFGLWINRGKMIFQKGIGSMTELYIVGKSAGNDFLAITNNGSICLYNTHWNTNMDIKGHGCVAVGSDSRLEISFARGENAVQNTQTIYLESPASVLAISGLTSLLTPPFINIAGFGQHNWIDLDIEFNNLTTEYDYFGHSGLLVIKLSKRQVVQIQIGESYDLRYLKLTSGPAGSRLVYELPSPNTPPSACSCKPIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ81</accession>
    <name>A0A1A0GZ81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_135582</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709297.1">
        <property type="nucleotide sequence ID" value="XM_018854388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ81"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ81"/>
    <dbReference type="GeneID" id="30027364"/>
    <dbReference type="OrthoDB" id="4207594at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21615">
        <property type="entry name" value="RRM_SNP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022023">
        <property type="entry name" value="U1snRNP70_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF5">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12220">
        <property type="entry name" value="U1snRNP70_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="102"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="232"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA17000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="331" mass="37381" checksum="C6A65DD6DB6D6246" modified="2016-10-05" version="1">MADESEKYPPKIQKLFAPRLPLLYKKPIDHPPETRASPFITLLRKWKLHIEQYKAQYGQTGPLVQHQLSAKERLAESRRRQLHEWEDTEAFAENKFLKDPYRTVFVARLYYSMTELDLSKAFAQYGSIDSVRIVRHTETGASRGYGFVVFEREADAKNCIHELAPTGLAVDPPKGSSTRRTILVDMERGRLIRSWRPRRLDGGLGGRHYTLPSALHPKDASAAASGRRFNLSQNPYQLPAAPPRFQKRPHPDRYSPAAGAKRPATDSDHYPGILRHPAMTAYASTSQPPSLSSARAPDPSVKDKYAKYQNVGSHAASGETGRSIRSIREQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Q0</accession>
    <name>A0A1A0H1Q0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial glycine transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Solute carrier family 25 member 38 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_13872</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial glycine transporter that imports glycine into the mitochondrial matrix. Plays an important role in providing glycine for the first enzymatic step in heme biosynthesis, the condensation of glycine with succinyl-CoA to produce 5-aminolevulinate (ALA) in the miochondrial matrix.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>glycine(in) = glycine(out)</text>
            <dbReference type="Rhea" id="RHEA:70715"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709351.1">
        <property type="nucleotide sequence ID" value="XM_018854439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Q0"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Q0"/>
    <dbReference type="GeneID" id="30027415"/>
    <dbReference type="OrthoDB" id="1924968at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904983">
        <property type="term" value="P:glycine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03064">
        <property type="entry name" value="SLC25A38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030847">
        <property type="entry name" value="Hem25/SLC25A38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF3">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10 12" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="18"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="124"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="11">
        <location>
            <begin position="231"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA17956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="319" mass="34431" checksum="FF7F53F4DEE7656C" modified="2016-10-05" version="1">MSNLLVSTPAADLQPKKPGPSVHLLSGGAAGLVSSFTLQPLDLLKTRLQQQQKEVSGYRTSVTRELRKLAHVRDLWRGALPLTLRTSVGAGLYFTILSQARLYLDGLKVRAGPRAPRSSVLPRLSHWENLATGFVVRALVGVVTMPITVIKTRFESNMYSYNSLYEGFEGIYSDGPARGAPGSYRNFFRGTTATLARDCPYAGLYVLFYEGFKNDLFPPLLLLVDGAGSVLAGMNNTLAAVSAAAVSTTITAPFDAVKTRLQLASPLKPLLSIVAVTRQLAAEPGGVQNLFSGLSLRLGRKALSAGISWCIYEELLKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1V8</accession>
    <name>A0A1A0H1V8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709409.1">
        <property type="nucleotide sequence ID" value="XM_018858209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1V8"/>
    <dbReference type="GeneID" id="30031185"/>
    <dbReference type="OrthoDB" id="4086878at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="96" mass="10831" checksum="D7532EE121F5E6C1" modified="2016-10-05" version="1">MAQIRKQNKQERISKSRAAKASALTGLTVAFFLAFCYHVWWAVNAFYHNKFGSNLSNFMIIVGGLALNAYLSVQFLLLCETKLLSNGIDADHKKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1W5</accession>
    <name>A0A1A0H1W5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TMEM205-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_226840</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709416.1">
        <property type="nucleotide sequence ID" value="XM_018855188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1W5"/>
    <dbReference type="GeneID" id="30028164"/>
    <dbReference type="OrthoDB" id="1641132at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="TMEM205-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053009">
        <property type="entry name" value="Xanthocillin_Biosynth-Assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PLANTS LEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23241:SF102">
        <property type="entry name" value="LD23009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="TMEM205-like" evidence="6">
        <location>
            <begin position="14"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA18021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="164" mass="17736" checksum="B9BCE43000915DD6" modified="2016-10-05" version="1">MFSTSAKSAVNVILFSVAFGGGVMHSFIVSPIAFKHLPREEFGKLQHQVLPLYLLSQAAAPVLLSLTTPLGSKFATPVLAASAVAGALNYFWVLPRCNQIKAEKKKLVDAKRHEQIIDGNTVETEEFKALSKQFGKFHGISSLLNLLSLVTLGAYGVALGRRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1W6</accession>
    <name>A0A1A0H1W6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_47683</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709340.1">
        <property type="nucleotide sequence ID" value="XM_018858205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1W6"/>
    <dbReference type="STRING" id="869754.A0A1A0H1W6"/>
    <dbReference type="GeneID" id="30031181"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508800" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA17945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="516" mass="53716" checksum="68B4B9968CAD9BDF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H219</accession>
    <name>A0A1A0H219_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_27582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709398.1">
        <property type="nucleotide sequence ID" value="XM_018855339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H219"/>
    <dbReference type="STRING" id="869754.A0A1A0H219"/>
    <dbReference type="GeneID" id="30028315"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="171" mass="18977" checksum="7CF2DEF686C2B800" modified="2016-10-05" version="1" fragment="single">TKADFINIIQKKLQSVLSKQSLGALCVAVGVHEPTKGVNKDTKVQNNQLWISELGVIEQRYQKIHLFDINIPNGPILQESRSVEAGNKILCPFPVSDNAPGFKVGFSICYDIRFPELAARLRQMGANILTYPSAFTTKTGEAHWLELGRARAIDSQCYVVMAAQCGEHDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H232</accession>
    <name>A0A1A0H232_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_47742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709377.1">
        <property type="nucleotide sequence ID" value="XM_018858214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H232"/>
    <dbReference type="STRING" id="869754.A0A1A0H232"/>
    <dbReference type="GeneID" id="30031190"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="680"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA17982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="709" mass="82258" checksum="E67875551921B546" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H265</accession>
    <name>A0A1A0H265_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_33794</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the ER membrane protein complex (EMC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709443.1">
        <property type="nucleotide sequence ID" value="XM_018856399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H265"/>
    <dbReference type="STRING" id="869754.A0A1A0H265"/>
    <dbReference type="GeneID" id="30029375"/>
    <dbReference type="OrthoDB" id="28092at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026895">
        <property type="entry name" value="EMC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011678">
        <property type="entry name" value="EMC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21573:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07774">
        <property type="entry name" value="EMC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291859" description="ER membrane protein complex subunit 1" evidence="12">
        <location>
            <begin position="20"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="837"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="ER membrane protein complex subunit 1 C-terminal" evidence="13">
        <location>
            <begin position="652"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA18048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="866" mass="96661" checksum="74CC8D64BCD78803" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H283</accession>
    <name>A0A1A0H283_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fungal pheromone mating factor STE2G-protein-coupled receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_80142</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709458.1">
        <property type="nucleotide sequence ID" value="XM_018859177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H283"/>
    <dbReference type="SMR" id="A0A1A0H283"/>
    <dbReference type="STRING" id="869754.A0A1A0H283"/>
    <dbReference type="GeneID" id="30032153"/>
    <dbReference type="OrthoDB" id="5402633at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038038">
        <property type="term" value="C:G protein-coupled receptor homodimeric complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14939">
        <property type="entry name" value="7tmD_STE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.920">
        <property type="entry name" value="Pheromone alpha factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000366">
        <property type="entry name" value="GPCR_STE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027458">
        <property type="entry name" value="STE2_TM1-TM2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28009">
        <property type="entry name" value="PHEROMONE ALPHA FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28009:SF1">
        <property type="entry name" value="PHEROMONE ALPHA FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02116">
        <property type="entry name" value="STE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00250">
        <property type="entry name" value="GPCRSTE2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="358" mass="39619" checksum="B4E21B1F3AF6455E" modified="2016-10-05" version="1">MISDTDPAEVLLNFTVQDTVFAVSFGQLDSSLHDSIRMALAQGLAIGAGALLLFVSWFVIVNKKTPMFILHQVSLLLLIIRSGLYIGYLLGPFNSLSFTYTGLFYDYWDSFHMTIAVNTFYVLLIASIECILVYQIHVIFKSAKARSFGLVFLVVSAALAVVVVVLYIISITHNLMSVKARITDGYSEYASWENNLPFIMFAASINYTCVLLMAKLLIAVRTRRVLGLKQFDAMHILLIMSTQTCIIPSIMVIWSYTGSRSTVFLNLSVIIIVCNLPLSSLWASSANNSSTPSSCQNSFFSRTSTNDSADKTLSSIPISDFEKGTLAESISEAAGMHGDVDSIERILREVEQEQAYEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H285</accession>
    <name>A0A1A0H285_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCA tRNA nucleotidyltransferase, mitochondrial</fullName>
            <ecNumber evidence="7">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCA-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_47717</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Nucleotidyltransferase that catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs, which is necessary for the attachment of amino acids to the 3' terminus of tRNA molecules, using CTP and ATP as substrates. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded. The structural flexibility of RNA controls the choice between CCA versus CCACCA addition: following the first CCA addition cycle, nucleotide-binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709432.1">
        <property type="nucleotide sequence ID" value="XM_018858207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H285"/>
    <dbReference type="STRING" id="869754.A0A1A0H285"/>
    <dbReference type="GeneID" id="30031183"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000019">
        <property type="entry name" value="tRNA nucleotidyltransferase cca2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="14">
        <location>
            <begin position="44"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="15">
        <location>
            <begin position="213"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA18037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="542" mass="61293" checksum="79ABB24FCAD2D5AB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4T5</accession>
    <name>A0A1A0H4T5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Ribokinase</fullName>
            <shortName evidence="12">RK</shortName>
            <ecNumber evidence="2 12">2.7.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RBK1</name>
        <name evidence="15" type="ORF">METBIDRAFT_47499</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribose + ATP = D-ribose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13697"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:47013"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="12">Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the carbohydrate kinase PfkB family. Ribokinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbohydrate kinase pfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.15" evidence="2 12"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709624.1">
        <property type="nucleotide sequence ID" value="XM_018858177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4T5"/>
    <dbReference type="STRING" id="869754.A0A1A0H4T5"/>
    <dbReference type="GeneID" id="30031153"/>
    <dbReference type="OrthoDB" id="415590at2759"/>
    <dbReference type="UniPathway" id="UPA00916">
        <property type="reaction ID" value="UER00889"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004747">
        <property type="term" value="F:ribokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019303">
        <property type="term" value="P:D-ribose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01174">
        <property type="entry name" value="ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01987">
        <property type="entry name" value="Ribokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011877">
        <property type="entry name" value="Ribokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="14">
        <location>
            <begin position="2"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="245"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="278"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="329" mass="35887" checksum="2C3297E2087C6814" modified="2016-10-05" version="1">MITIIGSLNYDLVTFTDKVPEGGETLQANSFENHLGGKGLNEALACARLSLNGRSDVRMIGNIGADAFGRELKQTLVDANVDTKYVRTIKNQSSGVAVILVETSGENRILITAGANGDLKPTCEEFETFFEDSSTEGDFVILQNEFPHTLESIRWIKSHKPTINIAYNPSPFNRNYITSETLSKIDFLIVNEGEALDVAHCILNEEEMSQFHNTILQDKVKGYGSLAQKLQTQLDQQNISTVIITMGSLGSVFASKTREASFVQSQKIDNVIDTTGAGDTFFGGVILQLSDRQDIEVAIKFATAASGLAIQKKGAAEGIPHFEDVNKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X7</accession>
    <name>A0A1A0H4X7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA primase</fullName>
            <ecNumber evidence="10">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33607</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709513.1">
        <property type="nucleotide sequence ID" value="XM_018856349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X7"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X7"/>
    <dbReference type="GeneID" id="30029325"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.920.10:FF:000003">
        <property type="entry name" value="DNA primase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 10" id="KW-0639">Primosome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA18978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="462" mass="53402" checksum="D10CA1CCC1B97533" modified="2016-10-05" version="1">MTDTFDDTSITEEAVDKVHVNLSKPSESDMLFYYQRILPFKHIFKWLNHAVKPTKDFTMREFAYEFRLGAYQRYNSFSTADEFQKSVVSANPTRFEVGAIYTVNPKERKSLPKNAMKPISKELVFDIDLTDYDEIRTCCSKTDICTKCWKFIQVASEIISVALRDDFGFTNFIWVFSGRRGAHCWVSDKRARSLDEASRKTVVEYMNVLGSKSNKMGKSSLGIKKPFHPHVERSLDILKVRFSEIILEDQDPWCTSHGSEENWAQVEEMLAFIPEQTLRNSLKAKWKDQTLFSLSRQKWEDLNSVAQSVLKNKSQVAQLNEAKKDIIIYYMYPRLDIEVSKQMIHLLKSPFCIHPGTGNVCVPFDPSKNLSGNMEDDAYGFNPMTAPNLKKIQDEIETWDSQRAIRDSSEPAEGSDSPPKSVHDYEKSCLKPYTEYFSHYVNRLITEEMRDSGKRSSDPLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Z0</accession>
    <name>A0A1A0H4Z0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">18S rRNA biogenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_79976</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709550.1">
        <property type="nucleotide sequence ID" value="XM_018859154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Z0"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Z0"/>
    <dbReference type="GeneID" id="30032130"/>
    <dbReference type="OrthoDB" id="1911237at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00875">
        <property type="entry name" value="RNA_Cyclase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.20:FF:000004">
        <property type="entry name" value="18S rRNA biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016443">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03400">
        <property type="entry name" value="18S_RNA_Rcl1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="5">
        <location>
            <begin position="8"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="6">
        <location>
            <begin position="185"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="367" mass="40211" checksum="02FA061C8717B6FE" modified="2016-10-05" version="1">MSTSKLINFEGERNFRFRLVIATLAGTHIRISKIRSNDMNPGLRDHEVSFLRLLEAVTNGSHIEISYTGTTVIYRPGIIIGGQLTHACSGNKPIGYFLEPLLYLAPFLKKKFSVILKGLTSSDKSKDAGIEAIKWGLIPVMEKFGVREVELHILKRGCPPLGGGEVHFICNSLIAQPLTIHALETPKMSAIRGVAFCARVSPSIVNRVIDSARAVFKPTGIEVNITADVWRGENSGKSPGFGVTLVAESKKGWRIFAEGTGTAGSLPEDLGELVAYQLLDELVKSSCFGRNQLKLALAYMAIGKEDVGRVRIHKAQIDEDFIWVLRDIKLIFGTEAYLKDDADELQEDYMTASIKGSGFTSASKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Z4</accession>
    <name>A0A1A0H4Z4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_79706</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709681.1">
        <property type="nucleotide sequence ID" value="XM_018859113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Z4"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Z4"/>
    <dbReference type="GeneID" id="30032089"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005310">
        <property type="term" value="F:dicarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF9">
        <property type="entry name" value="DICARBOXYLIC AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="415"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="50"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="575" mass="63919" checksum="ED847211B9065FCA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H581</accession>
    <name>A0A1A0H581_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709771.1">
        <property type="nucleotide sequence ID" value="XM_018856296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H581"/>
    <dbReference type="GeneID" id="30029272"/>
    <dbReference type="OrthoDB" id="4026374at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="93" mass="10527" checksum="25F0F7543F85036E" modified="2016-10-05" version="1">MNESPPPGTPRKSGKRAPTTPLVPSKTQNKKTSTAKPEPAFPRLLVMDVSIPPQAYEKELLQSKFQVWVLEKRLKEQTDLANFLELERSFVHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H583</accession>
    <name>A0A1A0H583_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_47176</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709645.1">
        <property type="nucleotide sequence ID" value="XM_018858133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H583"/>
    <dbReference type="STRING" id="869754.A0A1A0H583"/>
    <dbReference type="GeneID" id="30031109"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.770:FF:000001">
        <property type="entry name" value="60S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="15357" checksum="AC1B9CCB4FFA82BD" modified="2016-10-05" version="1">MVKFIKSGKVAIVTRGRFAGKKVVVVRPHDDGTKSHSFSHAIVVGVERGPLKVTKAHSAKQLAKRTKVKAFVKLVNYNHLMPTRYSLDVESFKNSVTPETLKEPTQKEEAKKVVKKALEEKHQAGKNKWFFSKLNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B1</accession>
    <name>A0A1A0H5B1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SIN1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_75290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709643.1">
        <property type="nucleotide sequence ID" value="XM_018858428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5B1"/>
    <dbReference type="GeneID" id="30031404"/>
    <dbReference type="OrthoDB" id="241990at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031567">
        <property type="entry name" value="CRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008828">
        <property type="entry name" value="Sin1/Avo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031313">
        <property type="entry name" value="Sin1_PH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13335:SF1">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX 2 SUBUNIT MAPKAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16978">
        <property type="entry name" value="CRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16979">
        <property type="entry name" value="SIN1_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRIM" evidence="3">
        <location>
            <begin position="340"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="SIN1-type PH" evidence="4">
        <location>
            <begin position="726"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="646"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="676"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="698"/>
            <end position="708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="877" mass="99095" checksum="E7AE7A36515937B8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5C7</accession>
    <name>A0A1A0H5C7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_225494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709647.1">
        <property type="nucleotide sequence ID" value="XM_018855171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5C7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5C7"/>
    <dbReference type="GeneID" id="30028147"/>
    <dbReference type="OrthoDB" id="3980246at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053720">
        <property type="entry name" value="Psm_Assembly_Chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018854">
        <property type="entry name" value="Psome_chaperone_3/4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10448">
        <property type="entry name" value="POC3_POC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15455" checksum="966D593B38DA1D01" modified="2016-10-05" version="1">MNANDKLQAQKQTCTISASFGSDNIDFHSISFTDKLILNIMVNGAMDASFDIPLLTLSTINRSIQQEDSLGPEPVVLMGDPNNLKAQLIASQVGKVVSQLREPRNVILSISSRWFGKADGIAEGDFEKLMFVLQNVKTLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5I0</accession>
    <name>A0A1A0H5I0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cysteine proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_46597</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709705.1">
        <property type="nucleotide sequence ID" value="XM_018858058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5I0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5I0"/>
    <dbReference type="GeneID" id="30031034"/>
    <dbReference type="OrthoDB" id="5065855at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044613">
        <property type="entry name" value="Nep1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46468:SF1">
        <property type="entry name" value="SENTRIN-SPECIFIC PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="138"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="429" mass="48990" checksum="5EDC89DD66E40A8B" modified="2016-10-05" version="1">MLYVLNPYKGLCFSPEAHKEQQPVHSDGLDQFDWSSVLPEIEIEISPSPPDEGTGQPRKKGKEARKERKEAKIQRKALHIAHVAHDKLARAGLTSSSSSDMEDFNPYLAKKRITDLYSQITRTTKGEEDTMLFQFGLVDLYKSDVSYLMPGQWLNDNNISLVYEMIIKYFLKGEDFGFHIHLIFPALTQLFLHFPVEEDLQGILPMKDLAKLKMVFFPFNFIDTDDYVDLEDVNNGDHWALCVLSIPARKLFVYDSMGFDGENDDKLLRKLASRLQKALFKPNEKITIVNKQCDQQENFDDCGVFLIMFSCYLISQLMSGEPTDLDLADVKFNPMLARLKITEIIHKLSLEAQKKKGDGLCLESFTSCKIFLNHKCLHVCSSSLGISSVVKVLEFVLQIHLPCFIIKRKIIEGILALMEENVGLTQTKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5I7</accession>
    <name>A0A1A0H5I7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79722</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709699.1">
        <property type="nucleotide sequence ID" value="XM_018859119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5I7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5I7"/>
    <dbReference type="GeneID" id="30032095"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071920">
        <property type="term" value="C:cleavage body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033620">
        <property type="term" value="C:Mei2 nuclear dot complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990251">
        <property type="term" value="C:nuclear exosome focus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106222">
        <property type="term" value="F:lncRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140517">
        <property type="term" value="F:protein-RNA adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033621">
        <property type="term" value="P:nuclear mRNA surveillance of meiosis-specific transcripts"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="84"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="39"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="22765" checksum="C9128C7F1CB8300E" modified="2016-10-05" version="1">MSDDVPVSVQNSYPTENQEQASQDHGSDPQLSFETSDGHVQIQLTLQDMEEEAARLRELNARLDALQSAPLGEEETQKSEVDPKSVYVGNVDYGATPLELQQHFSTSGEVERVTIITNKQTGQPKGFAYLEFSLADEANKAVATLDGSKLRERELKVNLKRTNIPGFSASSRGAFRGWGRALMRGRGRGGFRGRGFLRGTPRFSPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5N3</accession>
    <name>A0A1A0H5N3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peroxin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_46991</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat peroxin-7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709931.1">
        <property type="nucleotide sequence ID" value="XM_018858110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5N3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5N3"/>
    <dbReference type="GeneID" id="30031086"/>
    <dbReference type="OrthoDB" id="273771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062137">
        <property type="term" value="C:cargo receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005053">
        <property type="term" value="F:peroxisome matrix targeting signal-2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044536">
        <property type="entry name" value="PEX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46027:SF1">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="282"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="374" mass="41690" checksum="FB2C08D4BC9A6782" modified="2016-10-05" version="1">MLSYRTVGYNGYGVAYSPFFDNKLAVAAAANYGLVGNGKLFVLDIDSAGQIRENIAWDTQDGLFDLAWSELHENQVATASGDGSVKLFDLAVPQFPVMHFREHLREVFSVNWNLVDKVNFVTLSWDGSIKVWAPNRQQSLVTLSPQVDYTTAASSVPQTARPSVPMAHTNTPGPIDTAQCIYNAVFSPHSPLTIVSCTGSSHVHIWDIRAPKPLQLDYVAHGGLECLSVDWNKYKPTVIASAGTDKAVRVWDLRMIAKLDLPNLHLPMPAYHMRGPTPLNELLGHEYAVRKVAWSPHDGKELLSASYDMTARVWHDLSDERARFLNSKASGCRGVMRQHREFVIGCDYSLWGEPGWAATTGWDEMVYVWDTKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5P7</accession>
    <name>A0A1A0H5P7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="3 11">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_60608</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 11">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3 11"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709770.1">
        <property type="nucleotide sequence ID" value="XM_018858312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5P7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5P7"/>
    <dbReference type="GeneID" id="30031288"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="12">
        <location>
            <begin position="4"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="9">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="152" mass="16944" checksum="15142E83D2AF9BFF" modified="2016-10-05" version="1">MSNNERTFIAIKPDGVQRGLVSSILSRFENRGFKLVGIKLCQPTEALLRNHYDDLQEKPFFPSLLSYMLSGPVLATVWEGKDVVKQGRAILGATNPLQSAPGTIRGDFAIDMGRNVCHGSDSVESANKEIGLWFKNEELVDYKPALLSWIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Q6</accession>
    <name>A0A1A0H5Q6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="7">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25 26">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="ORF">METBIDRAFT_33542</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30 31">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Chromosome</location>
            <location evidence="4">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="30">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709898.1">
        <property type="nucleotide sequence ID" value="XM_018856324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Q6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Q6"/>
    <dbReference type="GeneID" id="30029300"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000745">
        <property type="entry name" value="Serine/threonine-protein kinase BUD32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0010">Activator</keyword>
    <keyword evidence="19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17 30" id="KW-0418">Kinase</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="20" id="KW-0779">Telomere</keyword>
    <keyword evidence="23" id="KW-0804">Transcription</keyword>
    <keyword evidence="21" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="29">
        <location>
            <begin position="15"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="EMBL" id="OBA19366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="250" mass="27855" checksum="3B4254D57F0C8DB7" modified="2016-10-05" version="1">MTDVILAQVQQAVPHIPMTVVSQGAEALVLSSTSHPYLDAKGKYIIKFRPKKPYRHPKIDALITKSRTVGEAKFMLKLNKLGVPAPHLILLDATNGVIWMNSVGDVLDDGSVSSLKNFLWYLEKNNGECLGSEAESYCRSTGSVIGMLHMHDMIHGDLTTSNIILQGDKSYLIDFGLSSYSNLPEDKAVDLYVLERAVLSTHSDYSDTYNKWLLEGYESAFSKSNKGKLKDTMKKLEDVRLRGRKRSMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5S9</accession>
    <name>A0A1A0H5S9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_46687</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709794.1">
        <property type="nucleotide sequence ID" value="XM_018858064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5S9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5S9"/>
    <dbReference type="GeneID" id="30031040"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="152"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="35491" checksum="6426A25BDC271663" modified="2016-10-05" version="1">MVKVTVCGAAGGIGQPLSLLLKLNKNVSELSLFDVVNVPGVGADLGHINSAATTKSFLPSSREDKTALAESLKGSDLVVIPAGVPRKPGMTRDDLFNINASIIQSLAQGIAENAPNALVLVISNPVNSTVPIVAETLKKNNVYNPARLFGVTTLDIVRANTFISQLFPGETKPTDFEVPVIGGHSGETIVPLYSVGAKPYYDKLSAEQKKELINRVQFGGDEVVKAKDGAGSATLSMAYAGYRLAESLLEAASGKTVVECTFLNLDSSIKGASEAKKLVNGLDFFSLPVKLSKDGIAEVNYEILEKIDDEEKRLLSVAIEQLSKNIEKGVAFGTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Z6</accession>
    <name>A0A1A0H5Z6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal recognition particle receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_79525</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SRP receptor beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710037.1">
        <property type="nucleotide sequence ID" value="XM_018859080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Z6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Z6"/>
    <dbReference type="GeneID" id="30032056"/>
    <dbReference type="OrthoDB" id="41266at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019009">
        <property type="entry name" value="SRP_receptor_beta_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09439">
        <property type="entry name" value="SRPRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508804" description="Signal recognition particle receptor subunit beta" evidence="11">
        <location>
            <begin position="31"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="281" mass="30936" checksum="7205AE911B940003" modified="2016-10-05" version="1">MDRIQQGLLAAVLGLILVLVVFQLQSRTDSKSGSSSPYCKPTYLIVGANGAGKTSLFYSLTNRTQNPASFVSSLEPNVASVRLPFANESIQKLYQLIDYPGHLKHSELLRTLILDDITVGKLKGVIYVVDSSSVSLSQELKVSAMAKELFLLLSTTEKNPNGVDFLFAVNKQDLFDSRPVHKVKQVLEDELAKLVADEISAKGLARGGSGIDNDDLEDDDEEKFRKEESTRDFWKAVVGSLRFSFDFLEGNMEFVGGSVLKGAVGNWENWFDEKAVNYGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L4</accession>
    <name>A0A1A0H6L4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_206803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710254.1">
        <property type="nucleotide sequence ID" value="XM_018855002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L4"/>
    <dbReference type="GeneID" id="30027978"/>
    <dbReference type="OrthoDB" id="4074674at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="267" mass="28775" checksum="3EA22C98A49BDC45" modified="2016-10-05" version="1">MIKSESRKSSLFSSLKTRRSLKDMSVRAQKSSLVISGPFPQPTPEARATPEPAHALSPLDPNAAVGKRYSQVYFAGPEGQENTQKRHSLAWQKHAAGLASELSLATLSAVPSDMASVDSALVDLPLPTIMEHDRRAPSLDLVGAAGLWRSAGSRGMGLGETCAPRRIHRTTNIGDMRKFIDGADMLPMSNRRSLEVEAHEIQTLGARACRRDFGVQCAVRLVENAFVMTFSAAGRGGPRDWDDVDEAEVLKGDADDDAADLLPVADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S3</accession>
    <name>A0A1A0H6S3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_32865</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710319.1">
        <property type="nucleotide sequence ID" value="XM_018856150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S3"/>
    <dbReference type="GeneID" id="30029126"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140898">
        <property type="term" value="P:CENP-A eviction from euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000021">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="11">
        <location>
            <begin position="15"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="12">
        <location>
            <begin position="99"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="133" mass="14409" checksum="52BC2D0C0D45E222" modified="2016-10-05" version="1">MSGKGKVHGGKGKSSEGNKATSSHSARAGLQFPVGRIKRYLKRNAQSKIRIGSKSAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDEELDNLIKATIAYGGVLPHINKALLLKVERKKGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H770</accession>
    <name>A0A1A0H770_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_45074</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710464.1">
        <property type="nucleotide sequence ID" value="XM_018857874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H770"/>
    <dbReference type="STRING" id="869754.A0A1A0H770"/>
    <dbReference type="GeneID" id="30030850"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="132"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="546" mass="61851" checksum="0AD316ED811EFEC1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H797</accession>
    <name>A0A1A0H797_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cyclophilin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_45522</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family. CWC27 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710494.1">
        <property type="nucleotide sequence ID" value="XM_018857928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H797"/>
    <dbReference type="STRING" id="869754.A0A1A0H797"/>
    <dbReference type="GeneID" id="30030904"/>
    <dbReference type="OrthoDB" id="442970at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF6">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="278" mass="31602" checksum="BBBB1A03017F75B4" modified="2016-10-05" version="1">MDIKPTAQVTLVTTKGNIDIQLFPKELPELCREFIRNCVNKKYVGLEFSKVTSSLIQTLQIDSSPEIKREKNSRIKFNTRGSVGLLRIENTRMSSANGFFISLDPISAYSADYVFIGNVVGDSIYNVVKIKDSELKPGTDMPLYSVRITDCVATQPYFDDLQERTSVLNLDKKRKKQKIAVSLQFEEENDENVGEQSEFVVRPAHELLIGNKSTKGSERVNTTTKETEPQIIEAKSLKISRAKGIRDPSIDPYQSDLDFSEDEISYETLRMHKFICRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7I1</accession>
    <name>A0A1A0H7I1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12889</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710377.1">
        <property type="nucleotide sequence ID" value="XM_018854275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7I1"/>
    <dbReference type="GeneID" id="30027251"/>
    <dbReference type="OrthoDB" id="4074036at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11597" checksum="1822E26FFF124BDC" modified="2016-10-05" version="1">MPIAQMSPERIEQINSQLLTAGAVGFLKGTIVALVSGSYFSYKYNFGHNKRFFLPQCKVGYFIAWGIVGITFAVENAKISVTKDLAEEENIQRQLYCKQELGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7J3</accession>
    <name>A0A1A0H7J3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710521.1">
        <property type="nucleotide sequence ID" value="XM_018856186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7J3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7J3"/>
    <dbReference type="GeneID" id="30029162"/>
    <dbReference type="OrthoDB" id="4024111at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="265"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="508" mass="55194" checksum="5FD47469C6AFBAFC" modified="2016-10-05" version="1">MDDYSGSALNTPMKKIRNLTIHSPMLTSPIRYPSMSDFPIDCDPINESMVEKGYSQTLSNAVLRELDTRAQEISSNVTVGSSTSKPMLVNRKRYSGIHKPLFSKMDSITSHYAAARQSDPTTQDISSSATKKRRTLNGPEDIFLASQEDPSPTRRKTHIKAGAPNGDAIANPSRPNPRNLSNDVFSARLQPLKLNVPLAASNPSKSALVSPTISSTRLLSSPIKTPGISPSKGSMNLHGLLLNDTIFAKPEGPVRIRQLSLQMAGVKPPEGGIPSLSRKTSSSELQRTRFGFDLQKKPSVDGLNRHYSVNGLQASSSVNSLQKKPSAGNLQKKPSIPSLQKKPFIPSLQKKPSIPSLDKKTSVSNLQKKKSIPSFKNTPKDSYVPSQICSGTFSSLLKGQKLPSHSNLAPDRLRDTSMLCESSSRPSSRNFMTSSGHATPSSSQFQSRGTKSNVTIPKPFSLYSKPTISSSQKTFGSQNSLNSAMSNASLLASQRSSNRFQKFKSRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7N3</accession>
    <name>A0A1A0H7N3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial ATPase complex subunit ATP10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32931</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710427.1">
        <property type="nucleotide sequence ID" value="XM_018856163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7N3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7N3"/>
    <dbReference type="GeneID" id="30029139"/>
    <dbReference type="OrthoDB" id="17089at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007849">
        <property type="entry name" value="ATP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28106:SF1">
        <property type="entry name" value="MITOCHONDRIAL ATPASE COMPLEX SUBUNIT ATP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05176">
        <property type="entry name" value="ATP-synt_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="35943" checksum="C568ACB52F986685" modified="2016-10-05" version="1">MGVLIDFPLERVETGCTRDSQAKCNAHHHQANPLSSDYLPMSARFFSVYVRAQQKKVPGFLKSALATSEPALTKPEVISRPFGFDEQIQLSQRKTSIRDMFSEEAKERRQRQLDYDLKHSSFYEFKSFNNTKGKIFQPPASYFKKEKSKFFPDLMAKTLSNNTHRLSDILKDRVTILRIFSTISGEKCANTYVEKYLTEEGYEEFCEKYPRAQIVDLNIPQTWAKGLFAGISKGNLRRKVNPARHDSYFLIPHGLLDLDAKTILKCGNTCAGYLYVLDSEGHIRWATSGNADEKELEVLWKSVRGISRELSTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7N7</accession>
    <name>A0A1A0H7N7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32938</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710438.1">
        <property type="nucleotide sequence ID" value="XM_018856165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7N7"/>
    <dbReference type="GeneID" id="30029141"/>
    <dbReference type="OrthoDB" id="4080273at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="100" mass="11070" checksum="A80D3AAA3102E9F5" modified="2016-10-05" version="1">MSGIGGKAFSIIAVGAIGWYTGVKFWRPLIIEQLEKDNNLDKGLVPPEYVDDAPKNWEDVKSLWKTSLHPEQHPLDVRGAEQAANIASSELAKIRENGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7T0</accession>
    <name>A0A1A0H7T0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_79382</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710606.1">
        <property type="nucleotide sequence ID" value="XM_018859054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7T0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7T0"/>
    <dbReference type="GeneID" id="30032030"/>
    <dbReference type="OrthoDB" id="432645at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_bL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="Ribosomal_bL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680:SF9">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL19M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="168" mass="19235" checksum="4D8DAEBCFD9EB769" modified="2016-10-05" version="1">MYGLARQIGQTASIFSKAVYEPLPKKRNGKSAAEYLHQQLLKKYDPTGKRTALVSGPERIRTGDVIKVTYLDRSTVLGRVLGVKRGVNNVGSNILIRNKISKVGCEIRIPLFNPEIKNIEIVQKPEKYLPRNKQYYIRNTKYDVGDLERGSFLFLNKEKIGKKREQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Y0</accession>
    <name>A0A1A0H7Y0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710726.1">
        <property type="nucleotide sequence ID" value="XM_018854197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Y0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Y0"/>
    <dbReference type="GeneID" id="30027173"/>
    <dbReference type="OrthoDB" id="3995714at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="511" mass="55407" checksum="B950F8A633239250" modified="2016-10-05" version="1">MYTHNQARASRAGALPPRPASPLSNNAVRFAVHNTQDPPKRAARVPGGEDPSPRPKGRRQPGGRCGHVTLLHSRAGAVPAEKSVPGLLRFFPSTGTCATPNGTSTPRKQQQANPCTSPTPRSEPLMKPQHTASSAATEPVNVKDPIRLAFLGGPRTGKTATILKLSCGTYRDTYYPLRKTTPVLFTYQAQSAALRLILDQHSSRRFLEHALARGDLLLSPVVSDALVRAAVKKTPLPGPAGDAPHGSQNEVYAAVWGDGDSDVARISPILAELIDTPAFNSSQFIPFLEASLHARLGKNVLRKLADTPRQPVNTEPLLVASGAGEMNGAIDGYFLFYSAVPSAEPPQYDELVAGQQAPGLGSSAAGSAQDTTLSLLPVIKAGLEEAWREYHTYRTRWDQEKEKDMFSLKTALRTMFDAHGSLQPGLLHHKLLATSLDPADPLCPPPIWIVCTHKNLPLASPTLIHDGKKLALLWRCGFYAMDVTENLDQVLALVIREVVERKSLQRARRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8I7</accession>
    <name>A0A1A0H8I7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32319</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710827.1">
        <property type="nucleotide sequence ID" value="XM_018856028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8I7"/>
    <dbReference type="STRING" id="869754.A0A1A0H8I7"/>
    <dbReference type="GeneID" id="30029004"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015343">
        <property type="term" value="F:siderophore-iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000197">
        <property type="entry name" value="Siderophore iron transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF92">
        <property type="entry name" value="GLUTATHIONE EXCHANGER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="458"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="576"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="651" mass="71905" checksum="0552F903E3F31A78" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8N1</accession>
    <name>A0A1A0H8N1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">A1 cistron-splicing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_78843</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710996.1">
        <property type="nucleotide sequence ID" value="XM_018858955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8N1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8N1"/>
    <dbReference type="GeneID" id="30031931"/>
    <dbReference type="OrthoDB" id="201752at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.550">
        <property type="entry name" value="Aar2, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007946">
        <property type="entry name" value="AAR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033648">
        <property type="entry name" value="AAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038514">
        <property type="entry name" value="AAR2_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033647">
        <property type="entry name" value="Aar2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038516">
        <property type="entry name" value="AAR2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689">
        <property type="entry name" value="A1 CISTRON SPLICING FACTOR AAR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12689:SF4">
        <property type="entry name" value="PROTEIN AAR2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05282">
        <property type="entry name" value="AAR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20981">
        <property type="entry name" value="AAR2_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAR2 N-terminal" evidence="3">
        <location>
            <begin position="9"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="AAR2 C-terminal" evidence="2">
        <location>
            <begin position="173"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="352" mass="40104" checksum="DB01949376779060" modified="2016-10-05" version="1">MNNRPPLGTLIIENVDRLGPNAVVGIDTQFFTVQNFLKGIKLIPAGHHLLHYSDSVEDGVSMRYAWWFSVHDGEALTVTWTEEKALFTPHDPLGLDLGDVYQYMVSYPEDPGKWARLCQYIDSEALEEYLPSLSQAISTATPLAEENMVLLDVLKSKKLDISVSDQSNEELKYTIIQPQKMRLHAFGEQLTRDALDRSWYLAELFGHDIDLLLAEIQVSFIHFVVLCNLCSYTQWMSLLALALTSKKYLRCHAEFSLRLLDLLQAQLETLPENYVLESLGTHVVERGELQAVFRNLNDIYEGSTSWHQLVSIAQRRFGLSTTETSMLDENLFEVYDLLGHDEEDEDAPALVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H950</accession>
    <name>A0A1A0H950_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_44333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710929.1">
        <property type="nucleotide sequence ID" value="XM_018857777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H950"/>
    <dbReference type="STRING" id="869754.A0A1A0H950"/>
    <dbReference type="GeneID" id="30030753"/>
    <dbReference type="OrthoDB" id="10249920at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000016">
        <property type="entry name" value="ADP-sugar pyrophosphatase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF26">
        <property type="entry name" value="ADP-RIBOSE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="51"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="22628" checksum="078B64C97651CD50" modified="2016-10-05" version="1">MKNSPFKAHVDKVQPMEQGKWIQTRKIDYTDPDGKSRVWEMAVRTTRTETTGLDAVSILGLLQKPGSKGKHVVLTKQFRPPVGKVVVELPAGLIDPNESVATTAERELLEETGYHGKVTKESIPLYSDPGLTNANMALATVDIDMADPRNTDPQPQLEDGEYIEVFTLPMAGLYDGLLAVCEKEGTTVDARLFHLAEGLRLADALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H979</accession>
    <name>A0A1A0H979_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ubiquitin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_17517</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710955.1">
        <property type="nucleotide sequence ID" value="XM_018854718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H979"/>
    <dbReference type="STRING" id="869754.A0A1A0H979"/>
    <dbReference type="GeneID" id="30027694"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000014">
        <property type="entry name" value="Ubiquitin-60S ribosomal L40 fusion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="8913" checksum="58CAF34ADB24268F" modified="2016-10-05" version="1" fragment="single">MQIFVKTLTGKTITLEVEASDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9C5</accession>
    <name>A0A1A0H9C5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32594</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711143.1">
        <property type="nucleotide sequence ID" value="XM_018856094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9C5"/>
    <dbReference type="GeneID" id="30029070"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="96" mass="10987" checksum="2FD7C91DE25A239A" modified="2016-10-05" version="1">MPKSVRPVASIWAFLNAALFLKSRPRTLVGQTDHNRRQLAKIQTSQEIWRGRLAMHRLCLQDRAIKQVSKSRLTGRQELPHMSKVFAGPPEIPADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9P5</accession>
    <name>A0A1A0H9P5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative tyrosine-protein phosphatase OCA1</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_12687</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Putative tyrosine-protein phosphatase required for protection against superoxide stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711258.1">
        <property type="nucleotide sequence ID" value="XM_018854257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9P5"/>
    <dbReference type="STRING" id="869754.A0A1A0H9P5"/>
    <dbReference type="GeneID" id="30027233"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000035">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF8">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE OCA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="35"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="198" mass="22420" checksum="6BDA88AEF69FA250" modified="2016-10-05" version="1">MSAATQQDPEIDPLDSSKSLLIPKAPILRIIPPLNFCPVEKQLYRSGQPSLINQSFLEELNLKTILWLASEEPVEDFLDYCLNKSINIEFVGLINDYNYTNINPWDSLDERTIKKALEFICNKDNYPLLVCCGMGRHRTGAVIGCLRRLQGWNLASVSEEYRRFSGARGGRILVELLIESFDVLSVEGVPSKLPDFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA30</accession>
    <name>A0A1A0HA30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_43307</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711254.1">
        <property type="nucleotide sequence ID" value="XM_018857643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA30"/>
    <dbReference type="STRING" id="869754.A0A1A0HA30"/>
    <dbReference type="GeneID" id="30030619"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="236" mass="26166" checksum="F675C1DBA79FE4EB" modified="2016-10-05" version="1">MQRHSPVVIEQNVSYTRGEYILPSPPKQHVAAPPEVQAAAESDEDSRFLRLAHEALVATSAESSLIVDPTIQDLLARLQYVLSPHGNPIKHSESIQLNESGQLMIQSFYEQFPDLSNNFFMDGPDTSVSSGKSADWVFPVETAATSLGSELSDAAGQRLARPARKFPCRGCDMLFRRSSDLKRHAKQHLTVPANICTQCGKGFARRDALKRHTGTLTCKRNAEKRLYYTNLGPGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAE3</accession>
    <name>A0A1A0HAE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S ribosomal protein L7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_11669</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711609.1">
        <property type="nucleotide sequence ID" value="XM_018854054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAE3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAE3"/>
    <dbReference type="GeneID" id="30027030"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005998">
        <property type="entry name" value="Ribosomal_uL30_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012988">
        <property type="entry name" value="Ribosomal_uL30_N_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01310">
        <property type="entry name" value="uL30_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08079">
        <property type="entry name" value="Ribosomal_L30_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30 N-terminal eukaryotes" evidence="6">
        <location>
            <begin position="7"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="82"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="241" mass="27612" checksum="483BDDE092996003" modified="2016-10-05" version="1">MATTLKPETQIKKTKTDQKTVEKRAADKAVRKAANKEKRKAICERTAAYNQEYRDAERAVIDAKRQARATNSFYVEAEPKLVFVIRIKGIMKIPPKPRKVMQLLRLTQINSGVFVKVTKATSELLKLAEPYVTFGYPNLATVRKLIYKRGHGKVRNQRVALSDNEIIEAALGKYGIVSIEDLIHEIYTVGPNFKQVNNFLWPFRLSNPNGGFRKRKFFHFIQGGDTGNREQFINNLVKQMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAF4</accession>
    <name>A0A1A0HAF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA polymerase</fullName>
            <ecNumber evidence="12">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_31525</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20 12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711367.1">
        <property type="nucleotide sequence ID" value="XM_018855871.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HAF4"/>
    <dbReference type="GeneID" id="30028847"/>
    <dbReference type="OrthoDB" id="6755010at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000510">
        <property type="term" value="F:H3-H4 histone complex chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05776">
        <property type="entry name" value="DNA_polB_alpha_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05532">
        <property type="entry name" value="POLBc_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3200.20:FF:000002">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000036">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2820:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3200.20">
        <property type="entry name" value="DNA Polymerase alpha, zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024647">
        <property type="entry name" value="DNA_pol_a_cat_su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038256">
        <property type="entry name" value="Pol_alpha_znc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045846">
        <property type="entry name" value="POLBc_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015088">
        <property type="entry name" value="Znf_DNA-dir_DNA_pol_B_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12254">
        <property type="entry name" value="DNA_pol_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08996">
        <property type="entry name" value="zf-DNA_Pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 12" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase alpha catalytic subunit N-terminal" evidence="17">
        <location>
            <begin position="12"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="15">
        <location>
            <begin position="458"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="14">
        <location>
            <begin position="770"/>
            <end position="1223"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger DNA-directed DNA polymerase family B alpha" evidence="16">
        <location>
            <begin position="1261"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="52"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="135"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="809"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF12254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA20857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1459" mass="163923" checksum="91B6F46979BAD8DF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAJ1</accession>
    <name>A0A1A0HAJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ALDH-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_171326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711530.1">
        <property type="nucleotide sequence ID" value="XM_018854695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAJ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAJ1"/>
    <dbReference type="GeneID" id="30027671"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF6">
        <property type="entry name" value="CYTOPLASMIC ALDEHYDE DEHYDROGENASE (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="4">
        <location>
            <begin position="39"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="485" mass="51043" checksum="C9C4FC7EDB3411F1" modified="2016-10-05" version="1">MSSAPRFGRISKRHVSCVPCVLSGKPVRTGLSIPVFSHTGEIVHHYSALENAPGHVSRICEEAQVGFLKWAARPADERRQILFAAAAIIEKHADLYVEAHREIGGPAGFARLGVAGAVAGCREYALQITNPDGVVLQLAAAQLAYTRQTPMGPVLAVAAWNAPAILWARAVLAPLAAGCSVVLKSLEKAPLPPYLLVQHLLQAGVDAQALQLVQVRAQDNAAVVESLLQNRRIKKVNFTGSTAVGRQVAAVAAHNLKPALLELGGKNVSVVCRDADLPKAAADALSSAWLHKGQVCMCLDTVYVDEHVCDEFVRVLQAEAARLCAENTDLRLNQRDAGAARGVHALVADAVAKGARVLHGGLPGHGPSNCSPVILTGVAGDMRLAAEESFGPVLSVEKFASVPAVVEHINDQPHGLKASVWSSDVMAAVAVASQLDFGGVHINGSTVHDEPTVPHGAVKESGSGRFNSVWGIEEFRYTKAITVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ6</accession>
    <name>A0A1A0HAQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78069</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711472.1">
        <property type="nucleotide sequence ID" value="XM_018858813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAQ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAQ6"/>
    <dbReference type="GeneID" id="30031789"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="473"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="569" mass="61618" checksum="DDF687E5468CF099" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ8</accession>
    <name>A0A1A0HAQ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">PLU-1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_41783</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711611.1">
        <property type="nucleotide sequence ID" value="XM_018857451.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HAQ8"/>
    <dbReference type="GeneID" id="30030427"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15518">
        <property type="entry name" value="PHD_Ecm5p_Lid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013637">
        <property type="entry name" value="Lys_sp_deMease-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08429">
        <property type="entry name" value="PLU-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ARID" evidence="10">
        <location>
            <begin position="180"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="597"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="1435"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="308"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1583" mass="176309" checksum="C739EA0A22B5785A" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAR6</accession>
    <name>A0A1A0HAR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA helicase</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_31787</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711724.1">
        <property type="nucleotide sequence ID" value="XM_018855918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAR6"/>
    <dbReference type="GeneID" id="30028894"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033203">
        <property type="term" value="C:DNA helicase A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043601">
        <property type="term" value="C:nuclear replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048761">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00376">
        <property type="entry name" value="IGHMBP2 family helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21138">
        <property type="entry name" value="SMUBP-2_HCS1_1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="217"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="718" mass="80717" checksum="EE5027AA7B462935" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAS6</accession>
    <name>A0A1A0HAS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711498.1">
        <property type="nucleotide sequence ID" value="XM_018855888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAS6"/>
    <dbReference type="GeneID" id="30028864"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291893" evidence="1">
        <location>
            <begin position="22"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="75" mass="8996" checksum="F7A20A94D546590B" modified="2016-10-05" version="1">MKWRVTIQMLFVFEMLLYGPGRQLFKELNKINWFVTTNFKVYTRRRGESQEAGSPSPLQPSELKRLLQETYPRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB76</accession>
    <name>A0A1A0HB76_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711750.1">
        <property type="nucleotide sequence ID" value="XM_018855924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB76"/>
    <dbReference type="STRING" id="869754.A0A1A0HB76"/>
    <dbReference type="GeneID" id="30028900"/>
    <dbReference type="OrthoDB" id="198652at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000165">
        <property type="entry name" value="HAD superfamily phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027706">
        <property type="entry name" value="PGP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010021">
        <property type="entry name" value="PGPP1/Gep4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01668">
        <property type="entry name" value="YqeG_hyp_ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF25">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROPHOSPHATASE GEP4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09419">
        <property type="entry name" value="PGP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="207" mass="23392" checksum="591BC9B87B818A33" modified="2016-10-05" version="1">MQLLNASAVTNISRLAYNPALCLPHLTVSSFDKIPLPIEIPGHTPEIKGIVLDKDNCFAKDHDDKVWPAYAEAWERLRQFYPKEKLLIVSNSAGTNDDVDYRQADKLERDTGVTVLRHPTKKPGCHQEILEYFAQHGINNASEIAVIGDRLFTDILMANMMGAWGVWLNEGVELSDKIFPRMERLLYAKLVTSREEPFMPSIPKDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBD2</accession>
    <name>A0A1A0HBD2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_180051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711831.1">
        <property type="nucleotide sequence ID" value="XM_018854763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBD2"/>
    <dbReference type="GeneID" id="30027739"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291773" evidence="2">
        <location>
            <begin position="18"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="216" mass="24348" checksum="0E24FE84F3826A9A" modified="2016-10-05" version="1">MSWQLIALACIKVLTHWTIDNPSPRVLTHWTNNNYMQFNTCVLHKESGNVSWALSINSNPCVCLVLMSCTRETVSSVQFSQALCQAAFREEYIHPVPLRDSTERMIRGRIDCLWQMYWGKGSVDKADMILACPLSQNPTQLLLSSELAAEKETTGTRIAFWPRYGCELLGDCPCQLKGELTQSKTAFRTHSHRVAAPSRSALKHPDTNHPDIIAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBF9</accession>
    <name>A0A1A0HBF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41122</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711722.1">
        <property type="nucleotide sequence ID" value="XM_018857357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBF9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBF9"/>
    <dbReference type="GeneID" id="30030333"/>
    <dbReference type="OrthoDB" id="2110361at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001158">
        <property type="term" value="P:positive regulation of L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051127">
        <property type="entry name" value="Fungal_SecMet_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424:SF6">
        <property type="entry name" value="PROLINE UTILIZATION TRANS-ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47424">
        <property type="entry name" value="REGULATORY PROTEIN GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="96"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="812"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="889"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="816"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="907"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="945" mass="106698" checksum="1744F81F5A8AD2FB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBG7</accession>
    <name>A0A1A0HBG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_41834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711840.1">
        <property type="nucleotide sequence ID" value="XM_018857457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBG7"/>
    <dbReference type="GeneID" id="30030433"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="65" mass="7443" checksum="D39E85A6F15C03B6" modified="2016-10-05" version="1" fragment="single">RVVRTKTSTNKKKIAAPQFRIWSPTTLLSVALYSLTNVDRTGNSASCMVWPQPKDWMLSKIYRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBT5</accession>
    <name>A0A1A0HBT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_12087</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712109.1">
        <property type="nucleotide sequence ID" value="XM_018854164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBT5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBT5"/>
    <dbReference type="GeneID" id="30027140"/>
    <dbReference type="OrthoDB" id="3251668at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF4">
        <property type="entry name" value="HYPOTHETICAL CONSERVED PROTEIN (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="172"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="267" mass="30631" checksum="C0FDF496B844AD80" modified="2016-10-05" version="1">MKSPMSSSHNPKASNPQLCVSSYFNNPSMEIHTIDKEYENGTNHSDNFLRQFSLPQENLQDFLTGFNAQEQAVHANQMDILHTRERTFDQFVLQDMFRVSLFPRTYNDVESSGMSSTSEDCASMNFSGEAYHLVSPAESRAIDTMADFTASSPSIKKKKHKRRLLMNRSRLGCWICRIKHLKCDETKPACTNCIRFGIECDYSVERPAYVLDSSLRRKKLDSIITKKRRRSESTPNASFFASAIEENMNQSDFSYFRKQEGTSFDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC30</accession>
    <name>A0A1A0HC30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Tricalbin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_70232</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712050.1">
        <property type="nucleotide sequence ID" value="XM_018858387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC30"/>
    <dbReference type="STRING" id="869754.A0A1A0HC30"/>
    <dbReference type="GeneID" id="30031363"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="13">
        <location>
            <begin position="205"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="398"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="547"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="674"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1007"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="840"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="840"/>
            <end position="867"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1215" mass="134504" checksum="7E3B0D176A5C5D30" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS6</accession>
    <name>A0A1A0HCS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Creatinase/aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_30829</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712302.1">
        <property type="nucleotide sequence ID" value="XM_018855721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCS6"/>
    <dbReference type="GeneID" id="30028697"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000004">
        <property type="entry name" value="xaa-Pro aminopeptidase 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000003">
        <property type="entry name" value="Xaa-pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="7">
        <location>
            <begin position="93"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="6">
        <location>
            <begin position="444"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="8">
        <location>
            <begin position="655"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="719" mass="80013" checksum="1A5DC24BC22CD38A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCT2</accession>
    <name>A0A1A0HCT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pru domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_67006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712405.1">
        <property type="nucleotide sequence ID" value="XM_018858362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCT2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCT2"/>
    <dbReference type="GeneID" id="30031338"/>
    <dbReference type="OrthoDB" id="340431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2020.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.70">
        <property type="entry name" value="Proteasomal ubiquitin receptor Rpn13/ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006773">
        <property type="entry name" value="Rpn13/ADRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044868">
        <property type="entry name" value="Rpn13/ADRM1_Pru"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038633">
        <property type="entry name" value="Rpn13/ADRM1_Pru_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038108">
        <property type="entry name" value="RPN13_DEUBAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225">
        <property type="entry name" value="ADHESION REGULATING MOLECULE 1 110 KDA CELL MEMBRANE GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12225:SF0">
        <property type="entry name" value="PROTEASOMAL UBIQUITIN RECEPTOR ADRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04683">
        <property type="entry name" value="Rpn13_ADRM1_Pru"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51917">
        <property type="entry name" value="PRU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pru" evidence="6">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="259" mass="28626" checksum="1121D8A36D60A75A" modified="2016-10-05" version="1">MTKTIRFNAGKVQYDDETKRCTPLPHRGVVTIAPSDEDEGFFDFCWAPKADSGLEKDELLLIPGDMTFRRVQSCKTGRVVALTFLSSGDKSLYWLQDVGDDEQLDRWTQKDEALVSAVQALITPADDGDDEAAADAAAVKEEPVPPRSTAADFFPVLLLSDVLSDELVLKHLRSMLPDELRARYGDMLPDAVEPSAEQIMAVVRSEFFRNSSAELSMNMTENNIGLILATSFGYEYSGEGIDAFLKGLRDSAKKSREDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCY4</accession>
    <name>A0A1A0HCY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Alternative oxidase</fullName>
            <ecNumber evidence="17">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_39969</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="16 17">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the alternative oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="17"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712372.1">
        <property type="nucleotide sequence ID" value="XM_018857211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCY4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCY4"/>
    <dbReference type="GeneID" id="30030187"/>
    <dbReference type="OrthoDB" id="16906at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009916">
        <property type="term" value="F:alternative oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010230">
        <property type="term" value="P:alternative respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.140:FF:000002">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.140">
        <property type="entry name" value="Alternative oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002680">
        <property type="entry name" value="AOX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038659">
        <property type="entry name" value="AOX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31803:SF3">
        <property type="entry name" value="ALTERNATIVE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01786">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005229">
        <property type="entry name" value="AOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12 16 17" id="KW-0408">Iron</keyword>
    <keyword evidence="14 17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005229-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA21876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="318" mass="37320" checksum="0D4C3635F7789C56" modified="2016-10-05" version="1">MKLGLTFTRCKSSLIFDVRTKVLENDSLEKNNDLKFITHPAFPHERWSDEDCAQVEYLHRPPKSRLDRIAFNSMATCKSLFNIVTGYKKPKTDAELQHRFKGTRWEMTENKWLTRVIFLESVAGVPGMVAAFLRHLHSLRLLKRDKAWIETLLDEAYNERMHLLTFIKIGQPSRFMRFFVYMGQGVFCNIFFFLYLLSPRYCHRFVGYLEEEAVSTYTHLIGDLSAKRLPKFDTVSVPKIAQDYWPELDEKSTFLDLVQRVRADESKHREVNHTLANLDQKCDRNPYAMKIEGVDKPQPEYGLKGENKVGWDKKDLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCY6</accession>
    <name>A0A1A0HCY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30817</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712289.1">
        <property type="nucleotide sequence ID" value="XM_018855719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCY6"/>
    <dbReference type="GeneID" id="30028695"/>
    <dbReference type="OrthoDB" id="276239at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015966">
        <property type="entry name" value="tRNA_lig_kin_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015965">
        <property type="entry name" value="tRNA_lig_PDEase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004:SF1">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08302">
        <property type="entry name" value="tRNA_lig_CPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08303">
        <property type="entry name" value="tRNA_lig_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase kinase" evidence="2">
        <location>
            <begin position="273"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase phosphodiesterase" evidence="1">
        <location>
            <begin position="456"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="704" mass="80951" checksum="D6C2187601EFE2E4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD71</accession>
    <name>A0A1A0HD71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Histone H4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_139921</name>
        <name evidence="13" type="ORF">METBIDRAFT_80146</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the histone H4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709462.1">
        <property type="nucleotide sequence ID" value="XM_018859179.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712416.1">
        <property type="nucleotide sequence ID" value="XM_018854466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD71"/>
    <dbReference type="STRING" id="869754.A0A1A0HD71"/>
    <dbReference type="GeneID" id="30027442"/>
    <dbReference type="GeneID" id="30032155"/>
    <dbReference type="OrthoDB" id="3972251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000007">
        <property type="entry name" value="Histone H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019809">
        <property type="entry name" value="Histone_H4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00417">
        <property type="entry name" value="H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00047">
        <property type="entry name" value="HISTONE_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 11" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="10 11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="12">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA18067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="11379" checksum="0A781066FE62CDDE" modified="2016-10-05" version="1">MSGRGKGGKGLGKGGAKRHRKILRDNIQGITKPAIRRLARRGGVKRISALIYEEVRGVLKSFLENVIRDAVTYTEHAKRKTVTSLDVVYALKRQGRTLYGFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDC8</accession>
    <name>A0A1A0HDC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712517.1">
        <property type="nucleotide sequence ID" value="XM_018857050.1"/>
    </dbReference>
    <dbReference type="GeneID" id="30030026"/>
    <dbReference type="OrthoDB" id="4018542at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019021">
        <property type="entry name" value="Mms22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09462">
        <property type="entry name" value="Mus7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="474"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1640" mass="187149" checksum="9D6652740F875686" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDS5</accession>
    <name>A0A1A0HDS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PLC-like phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712626.1">
        <property type="nucleotide sequence ID" value="XM_018855767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDS5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDS5"/>
    <dbReference type="GeneID" id="30028743"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08586">
        <property type="entry name" value="PI-PLCc_BcPLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF113">
        <property type="entry name" value="SI:DKEY-266F7.9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="1">
        <location>
            <begin position="13"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="298" mass="33918" checksum="3D462548FEF6DA81" modified="2016-10-05" version="1">MVNYAKWLGELGDNTPVGSLSIPGTHNSAACHIALPSVQCQGASVTDQLEHGVRFLDVRVGKHPLKSGNEAHELIVVHGKFPVRIPIPKKFADTLEEVYEFLDKHQSETVMVSIKQEGMGDWNHEQDEFANVIWEKFIEPNKNRWFLSTELPRLGDARGKAMLFRRFGVKNEERKRQYGFDAAWWKYNSIEDDRGSFVVQDFCEIQTKDDIPKKVTYVKSLAHKAAEHNATNSDNKLYVNFTLGSNFFNYSCWPKQVAHAMASHDLQDSFAKGSGIIVLDFVDDKDWGLIRGLIDKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE71</accession>
    <name>A0A1A0HE71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_31211</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712787.1">
        <property type="nucleotide sequence ID" value="XM_018855810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE71"/>
    <dbReference type="STRING" id="869754.A0A1A0HE71"/>
    <dbReference type="GeneID" id="30028786"/>
    <dbReference type="OrthoDB" id="434258at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12253">
        <property type="entry name" value="RRM_PIN4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001151">
        <property type="entry name" value="RNA-binding protein PIN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034186">
        <property type="entry name" value="PIN4-like_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="165"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="408"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="574"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="408"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="584"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="694" mass="74727" checksum="89C00F62CDCE9413" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEI3</accession>
    <name>A0A1A0HEI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_77755</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712809.1">
        <property type="nucleotide sequence ID" value="XM_018858754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEI3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEI3"/>
    <dbReference type="GeneID" id="30031730"/>
    <dbReference type="OrthoDB" id="5840532at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322">
        <property type="entry name" value="PKSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24322:SF736">
        <property type="entry name" value="RETINOL DEHYDROGENASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="313" mass="34467" checksum="0DED8DA369B5A54C" modified="2016-10-05" version="1">MPIAYLFSVDSLVHRLTQHRIWAAVALCGLWYLLALTNRIAKLPTSPLPPLGPNDVAIVTGGLSGLGLEMVKMLLQKHRLLRVFVLDITEPEPGLGDAVLFLRCDLASWQAVDAALSQISARCKALGRPVSVVINNAGVRLDGCLLAMARESIQRDFTVNTFAPVSILQRVVSEHVATHAHRRLSIVTVSSVLAALGPRNLLSYSASKAAATQFHEVLSRELAELPSVRMLLVMPGQLTTQMFSDLEPSRRFVAPLVDHRALAESILEKVELGEEGVMCEPFYANFGYLIKALPWKLQQMARDFSQMDEKIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEU4</accession>
    <name>A0A1A0HEU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_40296</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712991.1">
        <property type="nucleotide sequence ID" value="XM_018857255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEU4"/>
    <dbReference type="STRING" id="869754.A0A1A0HEU4"/>
    <dbReference type="GeneID" id="30030231"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="113"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="196"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="397"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="487" mass="53482" checksum="D0D92BEFD12FC041" modified="2016-10-05" version="1">MSNLLKRKNEDDSEVLPDQLSKRVALDSEQLAALEAADELAQTAPQFGAPNLAAIDEQIPVEHGAVSSENPEEGLTLASNQIHKLQLDQPPNSYAPTPVSKPVLSHREKEDLDHVYLRMFCPMKEAGVVVGKKGERITHIREKAGVRIQVSENLKNVPERVVSVKGLAENVARAFGLITRTILDEPEDEPALMMSKHYVLRLLVPHPVIGYVIGKGGGKFREIEENSAAKLKAAELVLPYSTDRVLSVSGVADAIHIAVYYISQVLLEHKETLKKNKIIFYNPANFHHLGTANNPMMNMAVAGTHVPASMPYIAQGPSMQMPGMAPMKEHVQPQPYHQKAYDFNMMFQPAVRPQYNGPLNLPANSIPLQSLYTDEHGNTIIGDVIVNLPVQISTGPDRFTQDVFVANTNIGSVIGKGGNNIKQIRESSGCAYVKIEPDQHQSILLGGGRGLTNIRKLTLTGTYSAIQTAIFFINQRISTDKERNGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEW6</accession>
    <name>A0A1A0HEW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_37168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713150.1">
        <property type="nucleotide sequence ID" value="XM_018856833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEW6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEW6"/>
    <dbReference type="GeneID" id="30029809"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="8">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="68" mass="7823" checksum="20C110B67AE444F6" modified="2016-10-05" version="1">METIYDEIEIEDFTFDPVTQLLQYPCPCGDRFAIALDDLRDGENIAVCPSCSLMVRVIFEPEDLDDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF04</accession>
    <name>A0A1A0HF04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_155596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713056.1">
        <property type="nucleotide sequence ID" value="XM_018854610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF04"/>
    <dbReference type="GeneID" id="30027586"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="240" mass="26263" checksum="8A3EA7C223BCE0AD" modified="2016-10-05" version="1">MRYLGQPARSGRRAFDWWWQKKEVIRRDYAITLAAGGRRAGHVTRRAWRWAPAPAFCAILPDGRGPGLLDAAPGGRFRRWGGLGREWGGAGKEGGAEIGLCAGPGYRRAGSDSPRYQRLSAGGALMLVGAPETRCTYLVTGFGSQRDLKIAGMGNQCDLGVGGSVKSGVFADGILGADGILVWCDLVIRGIWVLADLIIGGFGYWRIWILTALDTDSFGYWKIWILANLGTDRCYWWSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF08</accession>
    <name>A0A1A0HF08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713082.1">
        <property type="nucleotide sequence ID" value="XM_018858793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF08"/>
    <dbReference type="STRING" id="869754.A0A1A0HF08"/>
    <dbReference type="GeneID" id="30031769"/>
    <dbReference type="OrthoDB" id="7722975at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000005">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.740:FF:000001">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000043">
        <property type="entry name" value="Ser/Thr protein phosphatase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.780.10">
        <property type="entry name" value="5'-Nucleotidase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036907">
        <property type="entry name" value="5'-Nucleotdase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053828">
        <property type="entry name" value="Nucleosidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21953">
        <property type="entry name" value="NadN_nucleosid_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55816">
        <property type="entry name" value="5'-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="8">
        <location>
            <begin position="42"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="829" mass="93040" checksum="B33BF87A56A6FEDB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF12</accession>
    <name>A0A1A0HF12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial inner membrane protease subunit</fullName>
            <ecNumber evidence="7">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_11307</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase S26 family. IMP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712974.1">
        <property type="nucleotide sequence ID" value="XM_018853955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF12"/>
    <dbReference type="STRING" id="869754.A0A1A0HF12"/>
    <dbReference type="GeneID" id="30026931"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06462">
        <property type="entry name" value="Peptidase_S24_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000018">
        <property type="entry name" value="Mitochondrial inner membrane protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052064">
        <property type="entry name" value="Mito_IMP1_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019757">
        <property type="entry name" value="Pept_S26A_signal_pept_1_Lys-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383:SF16">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12383">
        <property type="entry name" value="PROTEASE FAMILY S26 MITOCHONDRIAL INNER MEMBRANE PROTEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00760">
        <property type="entry name" value="SPASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S26" evidence="8">
        <location>
            <begin position="14"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="188" mass="21074" checksum="0E66AE350DE41A5A" modified="2016-10-05" version="1">MKEAAIFAGSIFSWTIRAGCAAHFVNEYFYEFTETRGELMLTTLQAKRDFVHALKTHRLGRGLGMGDIVVATKPLDPDHRICKRISGMPGDVILVDPSSSSTLTNLPNTAVQNDGFNKYVEVPQGHVWVTGDNLSHSLDLRSYSWLPMGLIKGKVVAANSMDKGLWDEEGNFSFYNFRWLENLFVDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF86</accession>
    <name>A0A1A0HF86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chaperonin Cpn10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_75876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GroES chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713142.1">
        <property type="nucleotide sequence ID" value="XM_018858433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF86"/>
    <dbReference type="STRING" id="869754.A0A1A0HF86"/>
    <dbReference type="GeneID" id="30031409"/>
    <dbReference type="OrthoDB" id="184876at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00320">
        <property type="entry name" value="cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.33.40:FF:000002">
        <property type="entry name" value="10 kDa chaperonin, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.33.40">
        <property type="entry name" value="GroES chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00580">
        <property type="entry name" value="CH10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020818">
        <property type="entry name" value="Chaperonin_GroES"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037124">
        <property type="entry name" value="Chaperonin_GroES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018369">
        <property type="entry name" value="Chaprnonin_Cpn10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10772:SF0">
        <property type="entry name" value="10 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00166">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00297">
        <property type="entry name" value="CHAPERONIN10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00883">
        <property type="entry name" value="Cpn10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00681">
        <property type="entry name" value="CHAPERONINS_CPN10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="104" mass="11210" checksum="498AB4F11AFE0C4F" modified="2016-10-05" version="1">MSFLKNVNSLKPLFDRVLVQRMKPATKTSSGIYIPEKNQEKLNQATVIAAGPGVTDVATGKLIPVAVQAGDKVLLPNFGGAPVKVGEDEYLLFSDREILAKIDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFD0</accession>
    <name>A0A1A0HFD0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_38022</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713171.1">
        <property type="nucleotide sequence ID" value="XM_018856944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFD0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFD0"/>
    <dbReference type="GeneID" id="30029920"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041672">
        <property type="entry name" value="Bap31/Bap29_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF19">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18035">
        <property type="entry name" value="Bap31_Bap29_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="12">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Bap31/Bap29 cytoplasmic coiled-coil" evidence="13">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF18035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="191" mass="21149" checksum="D33DAEEFDB302350" modified="2016-10-05" version="1">MSIQMAMVFGALVAQMAVIALLLLPLPHMIRAKIVRGWAALRQNANYKVGLLFVSGLMVLQFADCVQKLQKYLRRESPEAVLNPSMGVGLLSDKLASKFYAQRNLYLSGAVLYLGLTIHTVLLIMGKLVAKEVLCRSAHNENTKDDSEEIVALKETIRKREVEIAAMKKQIEGVQKAYDGLSASSERSKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFG9</accession>
    <name>A0A1A0HFG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Cytochrome c oxidase subunit 9, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Cytochrome c oxidase polypeptide VIIA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_35498</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the fungal cytochrome c oxidase subunit 7a family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713375.1">
        <property type="nucleotide sequence ID" value="XM_018856616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFG9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFG9"/>
    <dbReference type="GeneID" id="30029592"/>
    <dbReference type="OrthoDB" id="2317211at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22888">
        <property type="entry name" value="CcO_VIIa_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014368">
        <property type="entry name" value="Cyt_c_oxidase_su7a_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28264:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28264">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000283">
        <property type="entry name" value="COX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="61" mass="6872" checksum="83C16F6718E81E17" modified="2016-10-05" version="1">MAIAPITGTLRKKIITDISVGFGIGFLLAGLYWRYEHMPIVQKRDAFYAKLKAEKEAEDSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG92</accession>
    <name>A0A1A0HG92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWR1-complex protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_22569</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713359.1">
        <property type="nucleotide sequence ID" value="XM_018855174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG92"/>
    <dbReference type="STRING" id="869754.A0A1A0HG92"/>
    <dbReference type="GeneID" id="30028150"/>
    <dbReference type="OrthoDB" id="4097064at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140849">
        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037651">
        <property type="entry name" value="Swc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058986">
        <property type="entry name" value="Swc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057558">
        <property type="entry name" value="Swc3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28108:SF1">
        <property type="entry name" value="SWR1-COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24707">
        <property type="entry name" value="Swc3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26242">
        <property type="entry name" value="Swc3_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWR1-complex protein 3" evidence="2">
        <location>
            <begin position="30"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Swc3 C-terminal" evidence="3">
        <location>
            <begin position="487"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="domain" description="Swc3 C-terminal" evidence="3">
        <location>
            <begin position="612"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="573"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="353"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF26242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="80304" checksum="089D04DCD8B35783" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGG8</accession>
    <name>A0A1A0HGG8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="A0A1A0HGG8"/>
    <dbReference type="GeneID" id="30028395"/>
    <dbReference type="OrthoDB" id="416585at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6773" checksum="4C42877B9D77C524" modified="2016-10-05" version="1">MALEPAVLEFSLALFIDDFSVALLKYFAKEITDRFLFPSNASNNSSWDWLDSGVLGSLWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGI8</accession>
    <name>A0A1A0HGI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_37759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018856910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGI8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGI8"/>
    <dbReference type="GeneID" id="30029886"/>
    <dbReference type="OrthoDB" id="5563754at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="PH_domain"/>
    </dbReference>
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        <property type="entry name" value="Skg3/CAF120-like_PH"/>
    </dbReference>
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        <property type="entry name" value="PH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="53"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="626" mass="69842" checksum="990CA7A43721A4E1" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGR1</accession>
    <name>A0A1A0HGR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_85370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713524.1">
        <property type="nucleotide sequence ID" value="XM_018859248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGR1"/>
    <dbReference type="GeneID" id="30032223"/>
    <dbReference type="OrthoDB" id="10561805at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="16318" checksum="AF3E385C7F1D5F51" modified="2016-10-05" version="1">MPNTNPTRSSKYWKHSNKNYVASTHAGAAIFLTAYLLCFFKTITWNLSKSDKEALFCLMAAFLAAGVAANLASMSAAAHFLVTTPVLAPLGNFGKTTGVKVTLAKAIAYTLHTLFWSMISTMVANLEPSTKTTRPNSTNLQLAVLTVLKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGR8</accession>
    <name>A0A1A0HGR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase assembly factor FMC1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_38113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713666.1">
        <property type="nucleotide sequence ID" value="XM_018856956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGR8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGR8"/>
    <dbReference type="GeneID" id="30029932"/>
    <dbReference type="OrthoDB" id="15893at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039196">
        <property type="entry name" value="Fmc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28015:SF1">
        <property type="entry name" value="ATP SYNTHASE ASSEMBLY FACTOR FMC1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15607" checksum="6AD0B1E368D5DCC4" modified="2016-10-05" version="1">MFKALYKELQKELLTAHKKVHLHRWKKDFEKNKARLTYDKMQLIRSRQSAEKVQAQLDALESGKAEIPPLDSSKVRNLLDSKEDLHNLQNVTAYLKNQRVYNELLERYNPGLTMSQGDNVRKTANMVGLSIPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS1</accession>
    <name>A0A1A0HGS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">4-nitrophenylphosphatase</fullName>
            <shortName evidence="5">PNPPase</shortName>
            <ecNumber evidence="3 5">3.1.3.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_38542</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57917"/>
            <dbReference type="ChEBI" id="CHEBI:61146"/>
            <dbReference type="EC" id="3.1.3.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.41" evidence="3 5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713671.1">
        <property type="nucleotide sequence ID" value="XM_018857017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS1"/>
    <dbReference type="GeneID" id="30029993"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000039">
        <property type="entry name" value="Phosphoglycolate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006349">
        <property type="entry name" value="PGP_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01460">
        <property type="entry name" value="HAD-SF-IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01452">
        <property type="entry name" value="PGP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="308" mass="33657" checksum="DC920BC165D98F1D" modified="2016-10-05" version="1">MSLKITTPQQARALLANYDYFLFDCDGVIWLGDHLLPSVAETLALLKKMGKAVIFVTNNLTKAREDYLRKFELLGIPGIQKSEVFGSSYALAVYVDKILRLPKDKKVWVLGESGIESELHEMGYQTLGGTDPALVENGVAFDPNHRLLTDLDPAVGCVVAGLTTNINYLKLSVTMQYLLKDGKLVPFVATNIDLTFPLKGKLLIGAGSIVETVSYASGRSPDAICGKPNQSMMQSILADRPGLKENPKRGLMVGDRLNTDMKFGRDGGLDTLLVLTGIETQDAVEKLDPAEAPTYFAEKLGDLFELTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH73</accession>
    <name>A0A1A0HH73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_76531</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714010.1">
        <property type="nucleotide sequence ID" value="XM_018858545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH73"/>
    <dbReference type="STRING" id="869754.A0A1A0HH73"/>
    <dbReference type="GeneID" id="30031521"/>
    <dbReference type="OrthoDB" id="4072599at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="178" mass="20772" checksum="F81E7D6525BFECA4" modified="2016-10-05" version="1">MWPFTSGDKSSDTLDKELPQSLKEFFKETDPSHRLNDLNEDPREAQVQEVLASQSNEYNDEFDQYKHRETPRKVASVNCAELLQAVVTCYRGWLFLGNDCSDEIVRTKICMEIQQEALKKLRYQDCCNKRHCTSIRAFTDKLFTAHFGQYGEKIDDETRARFDVALNEAFAAVWKKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHE7</accession>
    <name>A0A1A0HHE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylulose kinase</fullName>
            <ecNumber evidence="6">2.7.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_76450</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Highly specific D-xylulose kinase which participates in the catabolism of xylose. Xylose is a major component of hemicelluloses such as xylan. Most fungi utilize D-xylose via three enzymatic reactions, xylose reductase (XR), xylitol dehydrogenase (XDH), and xylulokinase, to form xylulose 5-phosphate, which enters pentose phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-xylulose + ATP = D-xylulose 5-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17140"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.17"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.17" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713907.1">
        <property type="nucleotide sequence ID" value="XM_018858531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHE7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHE7"/>
    <dbReference type="GeneID" id="30031507"/>
    <dbReference type="OrthoDB" id="1728974at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004856">
        <property type="term" value="F:D-xylulokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005998">
        <property type="term" value="P:xylulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07776">
        <property type="entry name" value="ASKHA_NBD_FGGY_SpXK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042024">
        <property type="entry name" value="D-XK_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF57">
        <property type="entry name" value="XYLULOSE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="7">
        <location>
            <begin position="317"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="606" mass="67858" checksum="CF0B15C2BED854F2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHN0</accession>
    <name>A0A1A0HHN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714150.1">
        <property type="nucleotide sequence ID" value="XM_018859488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHN0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHN0"/>
    <dbReference type="GeneID" id="30032463"/>
    <dbReference type="OrthoDB" id="4092398at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24819" checksum="0A080B5824050A74" modified="2016-10-05" version="1">MFQIARSNTAKFAVFQARCFTGAQLLSSQAAVASRKPVSPELKDLRAQLKLEKLVLNEMKIELRKILDNEKKKKKIALEKKREARALRPYKHITPLNLFVKENIKSIGALAETSQAWVKLSDAEKESYVEKAKKFNEDQLKIYTPKPVGPVPAYAKFLKSVWTAGADFGEASKAASLEWKKLTPEEKMTFSPSDKERAAYKLAYAAWKEERLRLANSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHU3</accession>
    <name>A0A1A0HHU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="19">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_9684</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">NADH-dependent reductase for DPH3 and cytochrome b5. Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. By reducing DPH3, also involved in the formation of the tRNA wobble base modification mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), mediated by the elongator complex. The cytochrome b5/NADH cytochrome b5 reductase electron transfer system supports the catalytic activity of several sterol biosynthetic enzymes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2 Fe(3+)-[Dph3] + NADH = 2 Fe(2+)-[Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71231"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:71232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="19"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713894.1">
        <property type="nucleotide sequence ID" value="XM_018859438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHU3"/>
    <dbReference type="GeneID" id="30032413"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000032">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000019">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF184">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18 19" id="KW-0274">FAD</keyword>
    <keyword evidence="5 18 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="40"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA23413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="283" mass="31280" checksum="87AACF2BCEF9F87F" modified="2016-10-05" version="1">MADEFNPLAVLATVVAIISSFIALYFVKKNKKPQPVLSPTEFRKFPLIEKIRVSHNSCIYKFGLPRSTDRLGLPIGQHISISAIIDGKEVVRSYTPISNDDQLGSFDLLIKTYENGNISRYVESKKIGETIDVRGPKGFFTYTPNMRKSLGMVAGGTGIAPMYQVLTAILDDPTDKTKIHLVYANVAENDILLKAELEALEAKHPEQLKIHYVLNNAPENWKGSVGFVTPEIMDTHLPKNHEDTNLLICGPPPMVSAIKKAAQTLGYPKAKAVSKLGDQVFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHV1</accession>
    <name>A0A1A0HHV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_50652</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase gamma chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714062.1">
        <property type="nucleotide sequence ID" value="XM_018858284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHV1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHV1"/>
    <dbReference type="GeneID" id="30031260"/>
    <dbReference type="OrthoDB" id="239812at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12151">
        <property type="entry name" value="F1-ATPase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.10:FF:000003">
        <property type="entry name" value="ATP synthase subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.80">
        <property type="entry name" value="ATP synthase, gamma subunit, helix hairpin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035968">
        <property type="entry name" value="ATP_synth_F1_ATPase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000131">
        <property type="entry name" value="ATP_synth_F1_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023632">
        <property type="entry name" value="ATP_synth_F1_gsu_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01146">
        <property type="entry name" value="ATPsyn_F1gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693">
        <property type="entry name" value="ATP SYNTHASE GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11693:SF22">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT GAMMA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00231">
        <property type="entry name" value="ATP-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039089">
        <property type="entry name" value="ATP_synthase_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00126">
        <property type="entry name" value="ATPASEGAMMA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52943">
        <property type="entry name" value="ATP synthase (F1-ATPase), gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00153">
        <property type="entry name" value="ATPASE_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="9 11" id="KW-0139">CF(1)</keyword>
    <keyword evidence="4 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="261" mass="28568" checksum="7EF2239556B41889" modified="2016-10-05" version="1">MRLKSIKNIEKITKTMKIVASTRLNKAQRAMQASRVFNESDTEFFKNAEPEENHSDKTLLIVVSSDKGLCGSIHTQISKSARKRIGELNGNVDVITIGEKAKVQLNRTHADKLKMAFSGVGKAEPTFHEVSLIADEIATLGKYENTEILYNKFVSGVSFEPSKFSVFAAEAIEKAPGLSKYELENEETPETVAEFSLANSLLTAQAEGYASEVSARRNAMDNASKNAGDMITANSILYNRTRQAVITNELVDIITGAASLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHY7</accession>
    <name>A0A1A0HHY7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_9865</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714097.1">
        <property type="nucleotide sequence ID" value="XM_018859476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHY7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHY7"/>
    <dbReference type="GeneID" id="30032451"/>
    <dbReference type="OrthoDB" id="3367at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045013">
        <property type="term" value="P:carbon catabolite repression of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051362">
        <property type="entry name" value="WD_repeat_creC_regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107:SF16">
        <property type="entry name" value="AT02583P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14107">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="696"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="707"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="868" mass="95743" checksum="B9574881883369A8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHY8</accession>
    <name>A0A1A0HHY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase DBP10</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP-dependent RNA helicase dbp10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_76475</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DDX54/DBP10 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713939.1">
        <property type="nucleotide sequence ID" value="XM_018858533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHY8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHY8"/>
    <dbReference type="GeneID" id="30031509"/>
    <dbReference type="OrthoDB" id="10261375at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17959">
        <property type="entry name" value="DEADc_DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000865">
        <property type="entry name" value="ATP-dependent RNA helicase DDX54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012541">
        <property type="entry name" value="DBP10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033517">
        <property type="entry name" value="DDX54/DBP10_DEAD-box_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08147">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01123">
        <property type="entry name" value="DBP10CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="142"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="382"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="19"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="365"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="859"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="878"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="916"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="938"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA23458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="947" mass="107082" checksum="0F4F603AD92CA33B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIE3</accession>
    <name>A0A1A0HIE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MADS-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_35033</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714256.1">
        <property type="nucleotide sequence ID" value="XM_018856560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIE3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIE3"/>
    <dbReference type="GeneID" id="30029536"/>
    <dbReference type="OrthoDB" id="1898716at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="96"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="191"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="415"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="446" mass="49219" checksum="62CA903A32B6AD5F" modified="2016-10-05" version="1">MGRRKIEIEPLGDDRTRTVTFVKRKAGLFKKAYELSVLCDVDMAVIIVGNDRVYEYLSVDTKELLKYYKHQSPYELKLPEDYGQFKKRHALKAGIRPAFEGDDTTERNAESDCESFSPEPKRQRTSSSPQNIYTKTPPVNSQFVQLAAPPKQMQRPLADVPLQRPVLRVQIPSDAKSQGGATESVTPIEGILGKKDNQPGSADGLSLQPPPLNTVRTSFNSKFRSPESRKQMPQLHVPMGKLQTLSPSTGAMPQLPINGSANFFSSLPPPSPSGQYPPSILPTPVFNQVFNQHYMNQMAGMPGLGPDASKLKPPLQHLFTNGEQTPVSGLPSRYLNEIFPSPSNMYPSQEWPTGLTPYTSTMSHFFTGMPPSANGATPHSLSAPFVHNQRTSLLVAGQRALPVRAALSLNVHAPTIQEDQQTHPSANTLPNLPSSEFMGQFYKKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ19</accession>
    <name>A0A1A0HJ19_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_37652</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714491.1">
        <property type="nucleotide sequence ID" value="XM_018856903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ19"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ19"/>
    <dbReference type="GeneID" id="30029879"/>
    <dbReference type="OrthoDB" id="2430343at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="127" mass="14066" checksum="5A306F8320D751BA" modified="2016-10-05" version="1">MNTFDKKIVVPDLLFENSFLRSLQRYADASVGKQSGPLADSELALSNDQSNNSNEPGHLNPVGVITPGIVVYAVIKDIMLRPLLEGFLWALTLMLFRPALRLVAAQGRHVGTWLATMLGRPPRRWAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ68</accession>
    <name>A0A1A0HJ68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_77041</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714680.1">
        <property type="nucleotide sequence ID" value="XM_018858627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ68"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ68"/>
    <dbReference type="GeneID" id="30031603"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03253">
        <property type="entry name" value="ABCC_ATM1_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="19">
        <location>
            <begin position="22"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="18">
        <location>
            <begin position="348"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA24199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="610" mass="67399" checksum="6C0B50276C6616D9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJL1</accession>
    <name>A0A1A0HJL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Aminopeptidase</fullName>
            <ecNumber evidence="9">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_35983</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714681.1">
        <property type="nucleotide sequence ID" value="XM_018856678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJL1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJL1"/>
    <dbReference type="GeneID" id="30029654"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="12">
        <location>
            <begin position="18"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="10">
        <location>
            <begin position="248"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="11">
        <location>
            <begin position="560"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="929" mass="105908" checksum="73432BFB5570209A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJM3</accession>
    <name>A0A1A0HJM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HLH-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_28357</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714677.1">
        <property type="nucleotide sequence ID" value="XM_018855353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJM3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJM3"/>
    <dbReference type="GeneID" id="30028329"/>
    <dbReference type="OrthoDB" id="71302at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11398">
        <property type="entry name" value="bHLHzip_scCBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047206">
        <property type="entry name" value="bHLHzip_scCBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47787:SF1">
        <property type="entry name" value="CENTROMERE-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="154"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="52"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="27738" checksum="D4CD907DEC72F116" modified="2016-10-05" version="1" fragment="single">GDHHSKKVKLGDSKKDDIAIDSELLGDQSSYSAGSGTGTENNKHDEDDEDAAVAAAASLVSDAETANAAAAALSHQQPHYHHHHHLQLHQQQQQQLHQKLAYESHYSQQLHAASAVNAAQQHHRSDNTILQPTTLPQQNSGNKPSHGSEEWHRARRENHKEVERRRRDSINQGIKELAQLIPSPDTNKAQILQRGIEYIRRLKENENNNIEKWTLEKLLTEQAVSELNASNEKLKSELEKAYREIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJT7</accession>
    <name>A0A1A0HJT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF1762-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_36002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IWR1/SLC7A6OS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714559.1">
        <property type="nucleotide sequence ID" value="XM_018856681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJT7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJT7"/>
    <dbReference type="GeneID" id="30029657"/>
    <dbReference type="OrthoDB" id="6255506at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040150">
        <property type="entry name" value="Iwr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013883">
        <property type="entry name" value="TF_Iwr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28063:SF1">
        <property type="entry name" value="RNA POLYMERASE II NUCLEAR LOCALIZATION PROTEIN IWR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08574">
        <property type="entry name" value="Iwr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor Iwr1" evidence="3">
        <location>
            <begin position="154"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="208"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="35"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="239"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="34732" checksum="4F69659BEFD4F835" modified="2016-10-05" version="1">MSQPPYVLRIKRKRGQDPLQALILEDAQRTKRSKPSSPISSNPRTPVPEARSWYFQLAHTDNQAAAPDAEVLASVLAEAASSRDKRRFVIPKAASEDDAAIPHELAEMLDSFLKVSDDGPPKRRRRGALNASGAGLASGAEQVPGETDTDDAVEEYVFDVYKLSTTEPLTDGNYPLSQIGYIRFFDDDEYDLMQSDDENKARTQIYLDDEDSNAESFYQNDYPEDEDAGTYSDTYEPPQADDGEDDYDENIGPVIIDSAEDAEGNAYDEDSADEAYERQHFFEGEDDDEMAIHRDKIFGRLQRMIDEQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK13</accession>
    <name>A0A1A0HK13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">BSD-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_134619</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715000.1">
        <property type="nucleotide sequence ID" value="XM_018854369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK13"/>
    <dbReference type="STRING" id="869754.A0A1A0HK13"/>
    <dbReference type="GeneID" id="30027345"/>
    <dbReference type="OrthoDB" id="360521at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13229">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3970.10">
        <property type="entry name" value="BSD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005607">
        <property type="entry name" value="BSD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035925">
        <property type="entry name" value="BSD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027079">
        <property type="entry name" value="Tfb1/GTF2H1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013876">
        <property type="entry name" value="TFIIH_BTF_p62_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12856">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03909">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08567">
        <property type="entry name" value="PH_TFIIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00751">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140383">
        <property type="entry name" value="BSD domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50858">
        <property type="entry name" value="BSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BSD" evidence="9">
        <location>
            <begin position="222"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="105"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="560"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="650" mass="73442" checksum="38C2C0C17AD86C47" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK38</accession>
    <name>A0A1A0HK38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Mitochondrial import inner membrane translocase subunit TIM44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_34891</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the Tim44 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714847.1">
        <property type="nucleotide sequence ID" value="XM_018856534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK38"/>
    <dbReference type="STRING" id="869754.A0A1A0HK38"/>
    <dbReference type="GeneID" id="30029510"/>
    <dbReference type="OrthoDB" id="10265990at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.240:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017303">
        <property type="entry name" value="Tim44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039544">
        <property type="entry name" value="Tim44-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10721:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037871">
        <property type="entry name" value="TIM44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Tim44-like" evidence="16">
        <location>
            <begin position="258"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="178"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA24366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="419" mass="47002" checksum="5D9765D23914F093" modified="2016-10-05" version="1">MFRQTLARQIRPASGKKLLSTSSVLCNENKGKSPLEVFFQTFKQEVKKSSELKENIKALQDERGRVAESEAFKRAKEAYDRAQKGSSAAGKVVKKTAEAVGGVAHKAWESPVGKGVRTTVLTGAEAADKAFEPVRKTQVYKDIGNVIDDGSSTAYGGFLTKEQREELRKKELQKRLQEGKGWKPVKENEEAGGALVATDSKPTGPSVGQKWEDFKVRSPVGRAIVLLKERWDESENGLISLVRTVFEKIAGFFSETEQAQVVKQFRMMDPTFRVTDFTRTLTNYIVPELLDAYMKNDQEVLKTWFSEAPFNVWQANNKQFIQQGLFSDGKILDIRSVDIVTCKMLQPNDTPVVVVSCRAQEIHLYRKAKTGEVAAGAEDHIQMSTYAMVFTRVPEEMDNETTEGWKVIEFARGGSRPFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK63</accession>
    <name>A0A1A0HK63_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ras-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30595</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714758.1">
        <property type="nucleotide sequence ID" value="XM_018855664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK63"/>
    <dbReference type="STRING" id="869754.A0A1A0HK63"/>
    <dbReference type="GeneID" id="30028640"/>
    <dbReference type="OrthoDB" id="4031310at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="310" mass="34799" checksum="47DF8331EF4991DB" modified="2016-10-05" version="1">MSNRHSQYNSPEFNSGYFNPYEARSSPASGHGPKVEFNPSAPSYQMLDQVSKNSAYSMKIVVVGDGGCGKTCMLVSYAQQKFPEIYVPTIFENYVSTVRSPGGKRIDLALWDTAGQEEYDRLRPLSYPDTDLILICFSVDNLVSLQNVKDTWYPEVNHFCPGIPVILVGTKSDLALTIDDALPMQIAQEINAVAYIQCSAKTMTNVKEVFDYALSRFQKDCEMAEQVDRLRKRLSILRGVPGHTRNNSSASYKKGHFKNASVNSSVLLDAPLTEEPYDSNPYAASPYQQKYNEEEFAFTRKKKSRKCAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKB1</accession>
    <name>A0A1A0HKB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_10580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714922.1">
        <property type="nucleotide sequence ID" value="XM_018853804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKB1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKB1"/>
    <dbReference type="GeneID" id="30026780"/>
    <dbReference type="OrthoDB" id="10255148at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="204" mass="24237" checksum="B74DE938D119A69D" modified="2016-10-05" version="1">MGAYKYLEELQRKKQSDVSRFLLRVRCWEYRQRNVIHRASRPSRPDKARRLGYKAKQGFVIFRIRVRRGGRKRPVPKGATYGKPTNQGVTQLKYQKSLRVTAEERVGRRAANLRVLNSYWVNSDSTYKYFEVILVDPSHNAIRRDARYNWIVNPVHKRREARGLTSAGKQSRGINKGHLYNNTKAGRRHTWKKHNTLSLWRYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKG2</accession>
    <name>A0A1A0HKG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial distribution and morphology protein 34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MDM34</name>
        <name evidence="13" type="ORF">METBIDRAFT_30739</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the ERMES/MDM complex, which serves as a molecular tether to connect the endoplasmic reticulum (ER) and mitochondria. Components of this complex are involved in the control of mitochondrial shape and protein biogenesis, and function in nonvesicular lipid trafficking between the ER and mitochondria. MDM34 is required for the interaction of the ER-resident membrane protein MMM1 and the outer mitochondrial membrane-resident beta-barrel protein MDM10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ER-mitochondria encounter structure (ERMES) or MDM complex, composed of MMM1, MDM10, MDM12 and MDM34.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="10">The ERMES/MDM complex localizes to a few discrete foci (around 10 per single cell), that represent mitochondria-endoplasmic reticulum junctions. These foci are often found next to mtDNA nucleoids.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Lacks alpha-helical transmembrane segments, suggesting that it resides in the membrane via beta-sheet conformations similar to those predicted for other outer membrane proteins and porin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MDM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714957.1">
        <property type="nucleotide sequence ID" value="XM_018855694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKG2"/>
    <dbReference type="STRING" id="869754.A0A1A0HKG2"/>
    <dbReference type="GeneID" id="30028670"/>
    <dbReference type="OrthoDB" id="17927at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21673">
        <property type="entry name" value="SMP_Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03105">
        <property type="entry name" value="Mdm34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027536">
        <property type="entry name" value="MDM34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058825">
        <property type="entry name" value="MDM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28185:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26545">
        <property type="entry name" value="Mdm34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="12">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="378"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="396"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="559" mass="62849" checksum="AE8B5D848E38F1C0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKJ0</accession>
    <name>A0A1A0HKJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_10642</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714987.1">
        <property type="nucleotide sequence ID" value="XM_018853815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKJ0"/>
    <dbReference type="GeneID" id="30026791"/>
    <dbReference type="OrthoDB" id="4080914at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="22648" checksum="C83AB479317D7E75" modified="2016-10-05" version="1">MDKPHNIHDSIQKLSTILHSQICEADAQIKSLEQNVQLLSRGQVVSDTTELEDDIPSDDEDIFIYELEKSRLQLVMDIQEENYISQKLKEMVGECETLMSSVKRYYNSFHKSRLQETLGYEVKEFDGTALEQIKNLLRVNTSRLEASQSALYNNAMKVLEVIESEETHLLAPKSQEALLKVIQQLNKKFNDLTEKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKU6</accession>
    <name>A0A1A0HKU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Superoxide dismutase [Cu-Zn]</fullName>
            <ecNumber evidence="7">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_134617</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="7">Binds 1 copper ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the Cu-Zn superoxide dismutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714998.1">
        <property type="nucleotide sequence ID" value="XM_018854368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKU6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKU6"/>
    <dbReference type="GeneID" id="30027344"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902693">
        <property type="term" value="C:superoxide dismutase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016670">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901856">
        <property type="term" value="P:negative regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00305">
        <property type="entry name" value="Cu-Zn_Superoxide_Dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000001">
        <property type="entry name" value="Superoxide dismutase [Cu-Zn]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018152">
        <property type="entry name" value="SOD_Cu/Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00068">
        <property type="entry name" value="CUZNDISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00087">
        <property type="entry name" value="SOD_CU_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00332">
        <property type="entry name" value="SOD_CU_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6 7" id="KW-0186">Copper</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="8">
        <location>
            <begin position="14"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="154" mass="15910" checksum="C1696DFBEBC0B2DD" modified="2016-10-05" version="1">MVKAVAVLRGDKSVSGVVTFEQATEAEPTIISWEITGNEPNALRGFHIHTFGDNTNGCTSAGPHFNPFAKTHGAPEDDERHIGDLGNIITDSEGVAKGTKQDLLVKLIGANSVLGRSVVVHDGTDDLGKGGHADSLKTGNAGGRPACGVIGLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYY4</accession>
    <name>A0A1A0GYY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DRS1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase drs1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_27588</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-binding RNA helicase involved in ribosome assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Associates with pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA16984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709281.1">
        <property type="nucleotide sequence ID" value="XM_018855340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYY4"/>
    <dbReference type="STRING" id="869754.A0A1A0GYY4"/>
    <dbReference type="GeneID" id="30028316"/>
    <dbReference type="OrthoDB" id="10259843at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17947">
        <property type="entry name" value="DEADc_DDX27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000842">
        <property type="entry name" value="ATP-dependent RNA helicase DRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="22">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="196"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="401"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="41"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="621"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="554"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="165"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="640"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="665"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="23">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="23">
        <location>
            <position position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00043999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA16984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="675" mass="75152" checksum="083CE58C2C0413D2" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4K5</accession>
    <name>A0A1A0H4K5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_47330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709506.1">
        <property type="nucleotide sequence ID" value="XM_018858152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4K5"/>
    <dbReference type="GeneID" id="30031128"/>
    <dbReference type="OrthoDB" id="4079124at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008698">
        <property type="entry name" value="NDUB7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05676">
        <property type="entry name" value="NDUF_B7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="57" mass="6767" checksum="DCE630F5369D3CD4" modified="2016-10-05" version="1" fragment="single">QEELETNKVPFVNRDKCAAHFISYYECLNKGTSYCNAAKDQFYECQYVALKQRLEKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4L6</accession>
    <name>A0A1A0H4L6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Riboflavin synthase</fullName>
            <ecNumber evidence="5">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33610</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709516.1">
        <property type="nucleotide sequence ID" value="XM_018856351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4L6"/>
    <dbReference type="STRING" id="869754.A0A1A0H4L6"/>
    <dbReference type="GeneID" id="30029327"/>
    <dbReference type="OrthoDB" id="10258924at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000004">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000006">
        <property type="entry name" value="Riboflavin synthase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006767">
        <property type="entry name" value="PRK09289.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098:SF0">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="RIBOFLAVIN SYNTHASE ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="10">
        <location>
            <begin position="106"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="11">
        <location>
            <begin position="106"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA18981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="233" mass="25563" checksum="BF1A7C1F9067748C" modified="2016-10-05" version="1">MFTGLIETIGTILEYTVLDNSSSGGNGVSMVVGNCHQILTDVHLGDSICTSGVCLTVTEFDEKKSWFKVGIAPETLRRSSLGDLAKGGLVNLERAVTSEVRLGGHVVQGHVDTIAHITGRKQDGNSIAFTFKLRDAQYIKYIVEKGFIAIDGTSLTVTHVDYKKHEFSIMLISYSQEKVILSRKPVGASVNIEVDYTGKLIEKQVELTLEGQLHQEDSPLVRFITETVKRHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4N2</accession>
    <name>A0A1A0H4N2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA-dependent ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47617</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709531.1">
        <property type="nucleotide sequence ID" value="XM_018858195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4N2"/>
    <dbReference type="STRING" id="869754.A0A1A0H4N2"/>
    <dbReference type="GeneID" id="30031171"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032392">
        <property type="term" value="P:DNA geometric change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18004">
        <property type="entry name" value="DEXHc_RAD54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="200"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="533"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA18996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="827" mass="92688" checksum="1B62CA3111DF6986" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4S5</accession>
    <name>A0A1A0H4S5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Phosphoribosylaminoimidazole carboxylase</fullName>
            <ecNumber evidence="4 11">4.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_33688</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H(+) = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + CO2</text>
            <dbReference type="Rhea" id="RHEA:10792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">In the C-terminal section; belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.21" evidence="4 11"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709614.1">
        <property type="nucleotide sequence ID" value="XM_018856381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4S5"/>
    <dbReference type="STRING" id="869754.A0A1A0H4S5"/>
    <dbReference type="GeneID" id="30029357"/>
    <dbReference type="OrthoDB" id="15425at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00130"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1970:FF:000013">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000037">
        <property type="entry name" value="Phosphoribosylaminoimidazole carboxylase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016301">
        <property type="entry name" value="Ade2_fungi/plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004679">
        <property type="entry name" value="PRK06019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609:SF5">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11609">
        <property type="entry name" value="PURINE BIOSYNTHESIS PROTEIN 6/7, PUR6/7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001340">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="13">
        <location>
            <begin position="109"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="560" mass="60928" checksum="280DB4EADB6565F7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4U1</accession>
    <name>A0A1A0H4U1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycerol uptake protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33583</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the membrane-bound acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709478.1">
        <property type="nucleotide sequence ID" value="XM_018856338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4U1"/>
    <dbReference type="STRING" id="869754.A0A1A0H4U1"/>
    <dbReference type="GeneID" id="30029314"/>
    <dbReference type="OrthoDB" id="420606at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051085">
        <property type="entry name" value="MB_O-acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285">
        <property type="entry name" value="ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13285:SF18">
        <property type="entry name" value="PROTEIN-CYSTEINE N-PALMITOYLTRANSFERASE RASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA18943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="269" mass="31149" checksum="DCDEA191304D9102" modified="2016-10-05" version="1">MYQSNYHPALSTKDLKRILVYGARLMFCVLVMEFLLHFMYVVAVSKTKAWEGDTPFQLSMLGMFNLNIIWLKLLIPWRLFRFWSLLDGIDPPENMIRCMDNNYSAVSFWRSWHRSFNKWVLRYIYIPMGGAGAGTGGYTSRVVNSLVVFSFVAIWHDIELKLLMWGWLVVLFLLPEIFATSYFKRYSNNSWYRHLCAIGAVVNIWMMMIANLYGFCLGHDGTIALLRAFFGTSNGLIYLVLSSGALFVGAQVMFEIREAEKRRGVDVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V4</accession>
    <name>A0A1A0H4V4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_47541</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709644.1">
        <property type="nucleotide sequence ID" value="XM_018858186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V4"/>
    <dbReference type="STRING" id="869754.A0A1A0H4V4"/>
    <dbReference type="GeneID" id="30031162"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010606">
        <property type="term" value="P:positive regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="169"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="512"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="854"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="977"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="854"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="901"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1028" mass="114506" checksum="46EFBFF5A25ED7D9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H503</accession>
    <name>A0A1A0H503_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_47314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709651.1">
        <property type="nucleotide sequence ID" value="XM_018858148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H503"/>
    <dbReference type="STRING" id="869754.A0A1A0H503"/>
    <dbReference type="GeneID" id="30031124"/>
    <dbReference type="OrthoDB" id="73901at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF27">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN CTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="208" mass="23096" checksum="79BC019F53A4DBEC" modified="2016-10-05" version="1">MASSTTLMDMSMSSMTGMSSLTASMDMSDMDMDMSSMSMNMYFSLAYEGYPVLFKGFSASNAGQAFGIFLLFFVASFVTKGFEFVKVYLELKVWNNPNYQTPQVTTIAGDCGCDDENEKVSDASEGYTPTGNQKQSSILTVVGRDTIRIVLCFLIEMFGYAMMLVAMTFSLVYFFAVVLGMAFGRVFFEKLSERLNIRPGANNFQGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H596</accession>
    <name>A0A1A0H596_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_47010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709786.1">
        <property type="nucleotide sequence ID" value="XM_018858114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H596"/>
    <dbReference type="GeneID" id="30031090"/>
    <dbReference type="OrthoDB" id="4082794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58100">
        <property type="entry name" value="Bacterial hemolysins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="41084" checksum="1EF76718A1280BDB" modified="2016-10-05" version="1">MSTFQPHTPSSSPKRKLRPESPHRDGTSTVGKLNFLQSDNIDEHFEHIHDSHTEILNYLQLLDAYTQKTQMDLDQLFDRLKNNNQHLSTLLSSIAEYSREVTEEGSATKTDVSRILNVLNDLRDSPNLEELMKRLSAKQEEILKEWRSYTAKSTTDLGKSVSINQHNILNKLQSIETQLGGIKIQNTFGEFRSRVESLAQENSKEVNAKLTEVLQALQLLNLKDKSHNPEVSLNDLKQADLQKEYNDLSRNYSDLEKSYTDLESRYSKLESRYDAKIQRFLELQSDFLKLGEESKNLKSSMADLDASKYDKVHELHSRRVRDLRNVTPLQRHNRIASMPLQNHFLKETNNSDLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5C2</accession>
    <name>A0A1A0H5C2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA exonuclease 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33705</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Exoribonuclease involved in ribosome biosynthesis. Involved in the processing of ITS1, the internal transcribed spacer localized between the 18S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709642.1">
        <property type="nucleotide sequence ID" value="XM_018856386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5C2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5C2"/>
    <dbReference type="GeneID" id="30029362"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06144">
        <property type="entry name" value="REX4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000007">
        <property type="entry name" value="Interferon-stimulated exonuclease gene 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037431">
        <property type="entry name" value="REX4_DEDDh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF45">
        <property type="entry name" value="RNA EXONUCLEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exonuclease" evidence="10">
        <location>
            <begin position="100"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="264" mass="29624" checksum="15E4BFB288399080" modified="2016-10-05" version="1">MASRNMSLSLNWKALSSRIGHKKPQEIKSKKLAKGHKYQAKDVSKLRKAQEIIRTASTKLLTPLEFALWARTSSSTFSVASSDAKIAVDYSDKKKSAPGKFLAIDCEFVGVGPNDQSALARVSLVNYYGVVLLDTYVKPEQRVTNWRTWVSGVTPQHMRNAVSYKEARERVKNIIDGRVLVGHAVHNDLECLGISVPKHLIRDTSRFPEFKKQNGGKMPSLKKLCRVYLNLEIQNGLHSSVEDAQATMAIFRLHQKAMESHFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5M9</accession>
    <name>A0A1A0H5M9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hexaprenyl pyrophosphate synthetase mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79787</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709792.1">
        <property type="nucleotide sequence ID" value="XM_018859126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5M9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5M9"/>
    <dbReference type="GeneID" id="30032102"/>
    <dbReference type="OrthoDB" id="9927103at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032478">
        <property type="term" value="C:heterotetrameric polyprenyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097269">
        <property type="term" value="F:all-trans-decaprenyl-diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF69">
        <property type="entry name" value="ALL TRANS-POLYPRENYL-DIPHOSPHATE SYNTHASE PDSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="441" mass="48317" checksum="7C4FBAD478C8FAB0" modified="2016-10-05" version="1">MVQPSKKTFDDPFSIVSHEMSNLAKLIANLIGSGHPILNRVSSYYFEAEGKNVRPLIVLLLSKALLQIPLEQRNRIVIDTADVSEQKQYAGVPQQTVVAGNSVNNSISPLAILHGINPRVILDPLSKPMDQLPELDAANGILPKQRRLAEIVEMIHTASLLHDDVIDLSDARRGRPSGNIAFTNKMAVLAGDFLLGRASVAIARLRNPEVIELLSTTIANLVEGEFMQLKNTVINNDNSTVTNDGDVKEIPHPTGKVPVKQHEYLVSAPVEVDHNTNVDAAFEYYSHKTYLKTASLMSKLSRAAAVLSGAQDNIIENCYEFGRNLGLCFQVVDDMLDYTSSDAAIGKPSQADLKLGLATAPILFAWREEPHLGELIGRKFSEPGDVEIARKAVDKWGGVEKTREMAQEYCAKALANLRVLPESDARSALELLTNSVLTRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Q2</accession>
    <name>A0A1A0H5Q2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein YTP1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_46518</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709775.1">
        <property type="nucleotide sequence ID" value="XM_018858050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Q2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Q2"/>
    <dbReference type="GeneID" id="30031026"/>
    <dbReference type="OrthoDB" id="4005299at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018825">
        <property type="entry name" value="DUF2427"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018827">
        <property type="entry name" value="YTP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685:SF3">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31685">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_6G12730)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10348">
        <property type="entry name" value="DUF2427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10355">
        <property type="entry name" value="Ytp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="522"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2427" evidence="3">
        <location>
            <begin position="40"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Protein YTP1-like C-terminal" evidence="4">
        <location>
            <begin position="250"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="63064" checksum="8AA6CC0240AA7177" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H614</accession>
    <name>A0A1A0H614_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_46522</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cclA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709879.1">
        <property type="nucleotide sequence ID" value="XM_018858051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H614"/>
    <dbReference type="STRING" id="869754.A0A1A0H614"/>
    <dbReference type="GeneID" id="30031027"/>
    <dbReference type="OrthoDB" id="10266026at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="101"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="51788" checksum="329DAA6095135911" modified="2016-10-05" version="1">MLSPKHALNHEENQLIPQTSKPEPDQRPILKTNKSELSELAIKKKQANVVVQPRLKPSVFAYDSIITEPVITSQHSVKDEAFFQFDDLPLNRRGYKYRVCKPNRALKATRYLTTELPPYKVRASYFDKSQGVGLTADMTSITTQNGWLSARCNVSMREGKYYFEYNIVNSNNGDDKAHVRVGIGRKELALDAPVGFDGYGYGLRDLNGQKITLSRPQEFMSEGFHTGDTIGFLVELPPLTRQRDGNSEFAKKFREKYPKSKKPTRAALNEEKEEKALNAFSNILRDQVAIKYKNGLFYEQFEYTRTKQMDHLLNPVTVFGETAVLEKKPDAQPPVPTIPDSRIVVYKNGQCVGEMFNELYSFLPLDTADDAASTEANTKQQQNPSYRNTDDGSLGYYPMMSVFQNGVVKINGGPDFKYAVPEGAQPLCKRYDEAVVEDWLWDLVDEVEAEYLDSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H646</accession>
    <name>A0A1A0H646_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13314</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710087.1">
        <property type="nucleotide sequence ID" value="XM_018854339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H646"/>
    <dbReference type="GeneID" id="30027315"/>
    <dbReference type="OrthoDB" id="4088875at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020999">
        <property type="entry name" value="Chitin_synth_reg_RCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187:SF1">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12273">
        <property type="entry name" value="RCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="196" mass="21757" checksum="D7B85087B1A3BD73" modified="2016-10-05" version="1">MPALHDIHHKLLAKRYYYGLTSSGARWAYFAIVAVALVLLIASIILVNRKRGRRGAKPIQGTQWMTPPSYYQSQTYYNQPTRRDPNMPSTYVPQYTETANAGDMGYYAPDGTFHPRSSKDGPEFPENAHQRTTANSDGQPIQAFDQTGTRVVADEDIDDFTRPVGPPPGSSGIDGDTTSSNSKEVERPDHPPPSMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H683</accession>
    <name>A0A1A0H683_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="2">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_33110</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709945.1">
        <property type="nucleotide sequence ID" value="XM_018856225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H683"/>
    <dbReference type="STRING" id="869754.A0A1A0H683"/>
    <dbReference type="GeneID" id="30029201"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF25">
        <property type="entry name" value="FERRIC_CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="13">
        <location>
            <begin position="367"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="629" mass="72467" checksum="300DF07F9E52DC53" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6D9</accession>
    <name>A0A1A0H6D9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase PFA4</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protein S-acyltransferase</fullName>
            <shortName evidence="11">PAT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Protein fatty acyltransferase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PFA4</name>
        <name evidence="14" type="ORF">METBIDRAFT_45729</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mediates the reversible addition of palmitate to target proteins, thereby regulating their membrane association and biological function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11 12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DHHC palmitoyltransferase family. PFA4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710182.1">
        <property type="nucleotide sequence ID" value="XM_018857954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6D9"/>
    <dbReference type="GeneID" id="30030930"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03199">
        <property type="entry name" value="DHHC_PAT_PFA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033682">
        <property type="entry name" value="PFA4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11 12">
        <location>
            <begin position="162"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="76"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="S-palmitoyl cysteine intermediate" evidence="11">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="391" mass="45431" checksum="51A51C14A6C76A96" modified="2016-10-05" version="1">MWEFRWPMLGIIIPCALIATIGYGSHFFIFSNHMSKRAQICYQVYVTMVWVSYILAIWKRPGTGPKSYVPPPSRWKRWCNKCNNFKPERAHHCRKCNVCVLQMDHHCPWTNNCVGNGNTPHFIRFLVWVIFATGLTLWELLKRAVGFWNDMHLPAYLIRKSELTAVILLVPLDAFVFFAVFVLFIKCILHLASGKTQIEVWESERIESQFHTERLWLKIRNNFKMVHNKEMPTLSSWNPTAAMYDSLELRDIHNEVDEPIVPQNFAPDDLIFPYDLGIYQNLKNALGQPWTWVLPWGNALGDGVKFKQNDDDDQLNLPWPPDGGNVEFTGRKLSDEELRKIGNVAVIKKLLDHRSNMSRNMWFNDWGEALGDYGVDVEAEEEKGLMIDSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Q7</accession>
    <name>A0A1A0H6Q7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33238</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMP24/GP25L family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710120.1">
        <property type="nucleotide sequence ID" value="XM_018856249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Q7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Q7"/>
    <dbReference type="GeneID" id="30029225"/>
    <dbReference type="OrthoDB" id="3427at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015720">
        <property type="entry name" value="Emp24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22811">
        <property type="entry name" value="TRANSMEMBRANE EMP24 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50866">
        <property type="entry name" value="GOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291674" description="GOLD domain-containing protein" evidence="9">
        <location>
            <begin position="18"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="10">
        <location>
            <begin position="27"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="213" mass="24630" checksum="378CABC3758989DB" modified="2016-10-05" version="1">MRISVLAVFVWVSHVTALHFYLLTGQTCCFYEDLTSDSLVVGKIDAYEYDERMDDYSKNPNLKLKITVDEIFDNDHRVVDQTSQPSGDFVFNSHDSGEHKFCLTPVYMDGTHGRKHRIFFDVAIGAALEYVDLKSTKKVDGLTLQIQDLNRKLQDIHMEQESIRERESVFRNQSESTNARVVWWSIIQVAVLVGTCTYQLRHLKGFFVKQKIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U8</accession>
    <name>A0A1A0H6U8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">oxaloacetate tautomerase</fullName>
            <ecNumber evidence="10">5.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_46041</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Tautomerase that converts enol-oxaloacetate, a strong inhibitor of succinate dehydrogenase, to the physiological keto form of oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>oxaloacetate = enol-oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:16021"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17479"/>
            <dbReference type="EC" id="5.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:16023"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.2.2" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710161.1">
        <property type="nucleotide sequence ID" value="XM_018857984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U8"/>
    <dbReference type="GeneID" id="30030960"/>
    <dbReference type="OrthoDB" id="74910at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050163">
        <property type="term" value="F:oxaloacetate tautomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000014">
        <property type="entry name" value="Uncharacterized mitochondrial hydrolase FMP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="12">
        <location>
            <begin position="11"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00056302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="231" mass="25678" checksum="A1BF5B591B27B242" modified="2016-10-05" version="1">MPLKYIENARKILCIGRNYAAHIKELNNTTPQQPFFFLKPSSSILKPNSGPFLVPRGTIVHHEVELAVILGKDLRNLTEDFSPEDALEAIDGYALSIDMTARNVQDEAKKKGLPWSIGKGFDTFLPLSEFIPKENIPDPYNVRLDLKVNGKTKQDDMASLMIFPIHKILSHMSSIMTLEKGDLILTGTPKGVGPVKPGDHISASLHVGPEKIASIEVEAREKDGPYYFKET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H728</accession>
    <name>A0A1A0H728_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pex24p-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_206478</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710228.1">
        <property type="nucleotide sequence ID" value="XM_018854992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H728"/>
    <dbReference type="STRING" id="869754.A0A1A0H728"/>
    <dbReference type="GeneID" id="30027968"/>
    <dbReference type="OrthoDB" id="74314at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052816">
        <property type="entry name" value="Peroxisomal_Membrane_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28304">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="TECPR1-like DysF" evidence="7">
        <location>
            <begin position="133"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="566" mass="65262" checksum="44500837FA7F8198" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H778</accession>
    <name>A0A1A0H778_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_230377</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710277.1">
        <property type="nucleotide sequence ID" value="XM_018855248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H778"/>
    <dbReference type="STRING" id="869754.A0A1A0H778"/>
    <dbReference type="GeneID" id="30028224"/>
    <dbReference type="OrthoDB" id="120967at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06876">
        <property type="entry name" value="PX_MDM1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013937">
        <property type="entry name" value="Sorting_nexin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08628">
        <property type="entry name" value="Nexin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00313">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51207">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="6">
        <location>
            <begin position="41"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="RGS" evidence="4">
        <location>
            <begin position="276"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="5">
        <location>
            <begin position="641"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="477"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="580"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="799"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="485"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="984" mass="112021" checksum="3254A304BDA711BF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7E0</accession>
    <name>A0A1A0H7E0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Six-hairpin glycosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_79327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710539.1">
        <property type="nucleotide sequence ID" value="XM_018859039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7E0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7E0"/>
    <dbReference type="GeneID" id="30032015"/>
    <dbReference type="OrthoDB" id="9984024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053169">
        <property type="entry name" value="MUG_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47791:SF3">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 191 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="394" mass="44971" checksum="1BDE1233962D3361" modified="2016-10-05" version="1">MTLTNPNLEAYSVTRGMWLEYYNSHRGSFYAKKKCSGCYNDNLFVIWAVSVAVQAVVDGARIFPELKPLIAPAVDTFRRYKNNQTKGYSAAENSGSDNDVYYDDDAQVASAIITAYEVTGDRKYLDSGRELVRFLMGGWDKKIGGVKWHVSKTYVNACTTAEVAKACLQLSKFIPEEASVYVEFATKCVEWQIEVLQDHGDKCIMDGIQPEGRKIDKVKLTYNTGTTLSAASHLYSITKSNKWKDIADNLAKAAINRNSPFYDRDYGHDKRYWRDPSYWTQLLIEGLADYLLYVGSDVPEDLLQKITLEVERHLKMFYKYLKDLKDGLYIQSFEPHRTFKEIYERDYKRQFGGKKGHGMRGDDKDGDKPQKCLMGLGSAARVFYQGARICPLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7I7</accession>
    <name>A0A1A0H7I7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710382.1">
        <property type="nucleotide sequence ID" value="XM_018854276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7I7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7I7"/>
    <dbReference type="GeneID" id="30027252"/>
    <dbReference type="OrthoDB" id="4087170at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="136"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="715"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="316"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="163"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="722"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="734" mass="83724" checksum="CCAF95F60A704D9C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7R1</accession>
    <name>A0A1A0H7R1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deoxyhypusine synthase</fullName>
            <ecNumber evidence="6">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33010</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710545.1">
        <property type="nucleotide sequence ID" value="XM_018856190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7R1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7R1"/>
    <dbReference type="GeneID" id="30029166"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000003">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="41549" checksum="894E7513DECE7BAE" modified="2016-10-05" version="1">MAQDDSRPSLATDAVLKQSEPIPEDFVEVAGIDYSKDNAYDMKATDLIKSMKTMGFQASSVGTACEIINEMSLWRGEHIDKVSEHEKTGEFDNLGHQKATIFLGFTSNLISSGIRDTLRYLVQHKKVSAIVASAGGIEEDLIKVLAPTYLGEFSLPGKSLRDKGLNRIGNLLVPNDNYCKFEDWIVPVLDKILEEQQAEAEKLGAEALNADSEAVWTPSRLIDRLGKEINDELSVLYWAHKNQIPVFCPAITDGSIGDMLFFHTYKASPQRLRLDIVSDIRRINAMSIAAFKAGMIILGGGLIKHHICNACLMRNGADWAVYINTGQEFDGSDAGARPDEAVSWGKIKAEAKSVKVYADVTVVWPLIVAATFAATRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7V0</accession>
    <name>A0A1A0H7V0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710626.1">
        <property type="nucleotide sequence ID" value="XM_018857904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7V0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7V0"/>
    <dbReference type="GeneID" id="30030880"/>
    <dbReference type="OrthoDB" id="270602at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02958">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14346">
        <property type="entry name" value="UBA_Ubx5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.300.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF6">
        <property type="entry name" value="UBX DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13899">
        <property type="entry name" value="Thioredoxin_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="2">
        <location>
            <begin position="390"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="345"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="477" mass="53917" checksum="C15545BAD459A877" modified="2016-10-05" version="1">MDDSIDTFLAFTATDDPALAKQFLDVSGGNLEYAVQLYMESGSNQAAPLASRNDEELAQRLQLEAYEDNGVREADANVHRHETLLDSFPAYDSGVDHARAIFGAGRVGIFNQRFDEDGDYGDQFDENLDMNDSESEDQIVELHLDEEEENIIEVDLDEEPISQPNRRLRTQRERLNELTSTQRRLAELFKPPFDLILRTSLDGARKEGREQKKWILVNIQDPTEFQCQVLNRDFWSDPSIKEIVRSEFIFLQYQNDSPHGQSYLNFYSVDSCPHISILDPMTGERVFKWSDGVVPNVEEWVSDVELFLEKFSLLPGSSNPVVKHDVKIDPDAMTEEQQIEYAMKQSMGGSAGTIEKASSNSGSDNEMPLSVEESLDPFEAIEPKKHAEPGSSPLTRVQIRFPNGKRLIHKFDPEKDTVLTLYQWLKYVLSETEESEFGIGNEGFTISCAGKPKLLDCISQTIKEAGLANASILLEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H830</accession>
    <name>A0A1A0H830_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flavin reductase like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33032</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the flavoredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710578.1">
        <property type="nucleotide sequence ID" value="XM_018856199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H830"/>
    <dbReference type="STRING" id="869754.A0A1A0H830"/>
    <dbReference type="GeneID" id="30029175"/>
    <dbReference type="OrthoDB" id="10250990at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798:SF5">
        <property type="entry name" value="FLAVIN REDUCTASE LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33798">
        <property type="entry name" value="FLAVOPROTEIN OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin reductase like" evidence="6">
        <location>
            <begin position="83"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="285" mass="31942" checksum="C9CEA7E2C6D6CFA0" modified="2016-10-05" version="1">MTQAKAGLESKVNRNPHPDFARVERQRPSFDKSKKWQYTQIPDVEWKPGSGANSDEWKNYKKISIDPYQEGRSPVDNYKTLISAVVPRPIGFVSTIGENGKKNLAPFSYFTVVNHDPPIFTLGFSGGKGSHKDTCKNILETEELTINIISEWFVEAANFTSINSPLGTDEWKLSGLTPLESQKVKPPHVAESAFSIEAKLVHSHEWESKADPGKATGTLCIVEGVNFHAREDVINEDMNILDIAKLKPVARLGGITYSRTLDGYERLRPDFQKEVEEVDEVNNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H894</accession>
    <name>A0A1A0H894_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ras GEF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_44147</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710722.1">
        <property type="nucleotide sequence ID" value="XM_018857748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H894"/>
    <dbReference type="STRING" id="869754.A0A1A0H894"/>
    <dbReference type="GeneID" id="30030724"/>
    <dbReference type="OrthoDB" id="546434at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF354">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="38"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="7">
        <location>
            <begin position="539"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="6">
        <location>
            <begin position="816"/>
            <end position="1057"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1109" mass="125429" checksum="9302B0D102864A19" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8A8</accession>
    <name>A0A1A0H8A8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose transporter</fullName>
            <shortName evidence="5">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_213106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710653.1">
        <property type="nucleotide sequence ID" value="XM_018855060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8A8"/>
    <dbReference type="STRING" id="869754.A0A1A0H8A8"/>
    <dbReference type="GeneID" id="30028036"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00803">
        <property type="entry name" value="nst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="39104" checksum="D1A4184816FE0850" modified="2016-10-05" version="1">MGILLFYLIGQGFYLLKKKMDKNSYAPVAPGGASSLANSGPLSIACYCMASILMTVTNKYVLSGYSFNLNFLLLAVQSTVCIVTIATLKGLNVITYRQFNKEEARKWMPIALLLVAMIYTGSKALAYLSIPVYTIFKNLTIILIAYGEVLWFGGKVTSMTLGSFLLMVFSSVLATYGDSSANKSAEDIYTMYLGYFWMLVNCFASAAFVLFMRIRIKLTNFKDFDTMYYNNLLSIPVLLVATFLFEDWSAENLALNFPAENRTATLLAMVFSGASSVGISYCSAWCVRVTSSTTYSMVGALNKLPIALSGLIFFDAAVNFWSVSSIFIGFISGLVYAVAKQKQLRENAQQLPSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C6</accession>
    <name>A0A1A0H8C6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_73288</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710667.1">
        <property type="nucleotide sequence ID" value="XM_018858411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C6"/>
    <dbReference type="GeneID" id="30031387"/>
    <dbReference type="OrthoDB" id="5579088at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="253" mass="28741" checksum="94AB086D6A272B24" modified="2016-10-05" version="1">MRVSQVLLGIFILTIPLSLYGANYRSFVVNSSFLQKLNLTFITDYGILWFSGVYWGLTICECLLARKAQKWIMANKLSKYLFYLSFAVFFNVWCFGKLLVERLNIATGGHCSDSNTATTSMSECQNDPLLLWVNGFDLSGHYYFLTTLIVLLTDATSWVKNLGRPDLDFELSDQEFIGSDFTYKLRTVLTTLKRLSSMLIVLWIAEFIITSVFFHTTWEKAAGLLGIPIAWAIIFFADKVWSAQVTSEIGSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8L1</accession>
    <name>A0A1A0H8L1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710852.1">
        <property type="nucleotide sequence ID" value="XM_018857608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8L1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8L1"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
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        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 5"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07189">
        <property type="entry name" value="SF3b10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="84" mass="9819" checksum="3971B4214B71CC1E" modified="2016-10-05" version="1">MADKIRENHQYLRQKEIYAGIGNADTSREEFLTNIHRDTLASLAMHENLLMYNSVATNTHPLLLRQELIKKMVQPLQKPDDRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8N7</accession>
    <name>A0A1A0H8N7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L domain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_44129</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018857745.1"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 11-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="76087" checksum="A2BF82CFC5F19D0D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8S0</accession>
    <name>A0A1A0H8S0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Cytochrome b5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_43543</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Microsome membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018857673.1"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
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    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
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    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
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    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 13" id="KW-0349">Heme</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0492">Microsome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="14">
        <location>
            <begin position="7"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA20395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="126" mass="13538" checksum="CD14CCCC5C9D9888" modified="2016-10-05" version="1">MSDTTELKVYTSEQVKEHNTAEDLWIVYNGHVYDVTKYLDEHPGGEEVIVDCAGEDATEAFDDIGHSDDAHEILKDLLIGKLEGGAVKPAKGASSSSGQTEASSVPVPLIAAAIFAVFAGVYFYMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U5</accession>
    <name>A0A1A0H8U5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_198627</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710942.1">
        <property type="nucleotide sequence ID" value="XM_018854904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U5"/>
    <dbReference type="GeneID" id="30027880"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="110" mass="12824" checksum="66E5760C72FC32B6" modified="2016-10-05" version="1">MSPIGVVQKNTDYPLESAPKETENLEVRTESSCFWRVDRMLGVVPNAQNFETEILGRVEERTARNCVCAQLCIVQFCRLSNRPRQLKSSDKLHIQAERERRQVEVWRCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8V0</accession>
    <name>A0A1A0H8V0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_78812</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710947.1">
        <property type="nucleotide sequence ID" value="XM_018858946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8V0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8V0"/>
    <dbReference type="GeneID" id="30031922"/>
    <dbReference type="OrthoDB" id="1721126at2759"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.110">
        <property type="entry name" value="Siroheme synthase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028162">
        <property type="entry name" value="Met8_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028281">
        <property type="entry name" value="Sirohaem_synthase_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14823">
        <property type="entry name" value="Sirohm_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14824">
        <property type="entry name" value="Sirohm_synth_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Siroheme synthase central" evidence="7">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Siroheme biosynthesis protein Met8 C-terminal" evidence="6">
        <location>
            <begin position="192"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="260" mass="29444" checksum="1B0F72C81CFEF97B" modified="2016-10-05" version="1">MTDKDQTPGSLLLAWQVKDKHCLVIGGGDVGLSRVDHMIRANAKITVVTGDKTHPDLLERHRAGKLYSLIQRAYEPLDLTMYETEPSFAVDSYKDITAAHYAAINELVSNEHFACVCCCIDDPVLSAAIYYQCKYLRIPVNIADKPPMCDFYFGLMFNQDALQIMISTNGKSPRLLKMIKDDILKKFSNIRLNDAVDNLGAIRARLREKKVPDTDLASIDARMLWIKLVLELPTGALDKIIEPYPEFPPKDYEEFKKYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8X1</accession>
    <name>A0A1A0H8X1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA mismatch repair protein MSH3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA mismatch repair protein msh3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">MutS protein homolog 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_78831</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutS family. MSH3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710978.1">
        <property type="nucleotide sequence ID" value="XM_018858951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8X1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8X1"/>
    <dbReference type="GeneID" id="30031927"/>
    <dbReference type="OrthoDB" id="121051at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032302">
        <property type="term" value="C:MutSbeta complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000406">
        <property type="term" value="F:double-strand/single-strand DNA junction binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000404">
        <property type="term" value="F:heteroduplex DNA loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007534">
        <property type="term" value="P:gene conversion at mating-type locus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043570">
        <property type="term" value="P:maintenance of DNA repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000735">
        <property type="term" value="P:removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF122">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="15">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="922" mass="102004" checksum="BB7B9F1F00DAFCBD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Z0</accession>
    <name>A0A1A0H8Z0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">EKC/KEOPS complex subunit GON7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_43295</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. GON7 likely plays a supporting role to the catalytic subunit KAE1 in the complex. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GON7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710870.1">
        <property type="nucleotide sequence ID" value="XM_018857641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Z0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8Z0"/>
    <dbReference type="GeneID" id="30030617"/>
    <dbReference type="OrthoDB" id="2288868at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014849">
        <property type="entry name" value="EKC/KEOPS_Gon7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08738">
        <property type="entry name" value="Gon7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0779">Telomere</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="109" mass="11885" checksum="C53B488B1D125616" modified="2016-10-05" version="1">MPSLVPSATYESPLELEAVRFVPGEGPHSTDGKTTRISDIVIGAGGTDRDKPSAARDTDLGGLRARLTTVQDQLNEFLTSKMRNKRKLDDAEADVERRLLDEGVDEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H930</accession>
    <name>A0A1A0H930_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH:flavin oxidoreductase/12-oxophytodienoate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78785</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710909.1">
        <property type="nucleotide sequence ID" value="XM_018858941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H930"/>
    <dbReference type="STRING" id="869754.A0A1A0H930"/>
    <dbReference type="GeneID" id="30031917"/>
    <dbReference type="OrthoDB" id="72788at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303:SF4">
        <property type="entry name" value="NADPH DEHYDROGENASE C23G7.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="37142" checksum="4B613AF2AB6E13C2" modified="2016-10-05" version="1">MVLPNRVGVSPLCQYTGVNDIPTEYHKIHYGAFALRGAGLVMVEATTIIPHAGVSTVDVGLYNDKQEAKLKEIVDFAHANTSWIGIQLGHTGRKDWDPRGDEDKIVAPSALPFREGGQYPVPRALTVEEIHEIVKKFSESARRAVSAGFDFVEIHGAHGYLINEFMSAHSNKRTDQYGGSYENRIRFLLEFIDCVRANMPEGFPVLSDSNPDAWILEDSVRLAPIVVKHGVDVLDVSGGGNDANTDRLPKHFGMFMNVSEAVKKAVGDKAIVPGVGRLHDPVKVNELHEKGTIDFAFVGAPFMVNQGLVFDWAETLDVKLHHAPLLLPVRPEYAEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H946</accession>
    <name>A0A1A0H946_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein CASP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_43761</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CASP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711053.1">
        <property type="nucleotide sequence ID" value="XM_018857695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H946"/>
    <dbReference type="STRING" id="869754.A0A1A0H946"/>
    <dbReference type="GeneID" id="30030671"/>
    <dbReference type="OrthoDB" id="10257567at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012955">
        <property type="entry name" value="CASP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057476">
        <property type="entry name" value="Cux_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043">
        <property type="entry name" value="CCAAT DISPLACEMENT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14043:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN CUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08172">
        <property type="entry name" value="CASP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25398">
        <property type="entry name" value="CUX1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="616"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Cux N-terminal" evidence="13">
        <location>
            <begin position="10"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="CASP C-terminal" evidence="12">
        <location>
            <begin position="405"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="26"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="192"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="484"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="670" mass="77409" checksum="BB447B965389ABF2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9E2</accession>
    <name>A0A1A0H9E2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nagb/rpia/CoA transferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_205275</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in regulation of tRNA subcellular distribution.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711261.1">
        <property type="nucleotide sequence ID" value="XM_018854969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9E2"/>
    <dbReference type="STRING" id="869754.A0A1A0H9E2"/>
    <dbReference type="GeneID" id="30027945"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000017">
        <property type="entry name" value="6-phosphogluconolactonase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="7">
        <location>
            <begin position="92"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="360" mass="39962" checksum="29BD25D3416CA66E" modified="2016-10-05" version="1">MTTQVPLIYSFPGFEGVADAVADHIITAQNNTLYNTLDKAAIAGLKLAAALRPSLLQMPSSASIHGHSRSNSGGLANGEGSRRTPKLKKRSELRFKIAISGGSLIKILHQGLLSRQAEVEWDRWDVYFADERMVPFDSPDSNYAQAKQMIFDLIEGDKKPNVYHIDELLISDPQECADAYEKLLIDNFAKKDSVKLPMFNLLLLGCAPDGHIALLFPNHGEQLREDLAWCMPVSNAPLGPENRITLSIPVICHAQRVAFVVEGLTKAPIMRIIMERPEKGLPSSIVNEGAAGKVSWFVDDDALNDCYDITKKKYNLMTFGDRPVSAILWEHVSSCCKFHSPVVARRAAWPWSMNQITYVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9J7</accession>
    <name>A0A1A0H9J7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adenine phosphoribosyltransferase</fullName>
            <ecNumber evidence="8">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_32756</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711321.1">
        <property type="nucleotide sequence ID" value="XM_018856124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9J7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9J7"/>
    <dbReference type="GeneID" id="30029100"/>
    <dbReference type="OrthoDB" id="363185at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002055">
        <property type="term" value="F:adenine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000004">
        <property type="entry name" value="Adenine phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050054">
        <property type="entry name" value="UPRTase/APRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002636">
        <property type="entry name" value="PRK02304.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32315:SF3">
        <property type="entry name" value="ADENINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="14">
        <location>
            <begin position="49"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00008391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA20799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="185" mass="20155" checksum="D52199507CB017F9" modified="2016-10-05" version="1">MSPTILSLATELKSELKQFPNFPQEGILFEDFLPIFAKPDSFRKLVKAFEMHLQGQEVDYIVVLESRGFLFGPALALALNAGFIPVRKPGKLPGPVLSAEFVKEYGSDKFELQEHVIPKDAKVVVVDDILATGGSASAAGELVAKAGAKTVEYLFVMELDFLKGREKLDAPVFTLLSGQAEKLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9T6</accession>
    <name>A0A1A0H9T6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Chorismate mutase</fullName>
            <ecNumber evidence="4 15">5.4.99.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_32725</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the Claisen rearrangement of chorismate to prephenate. Acts at the first branch point in the aromatic amino acid pathway where it steers biosynthesis towards phenylalanine and tyrosine, and away from tryptophan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13898"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="4 15"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711298.1">
        <property type="nucleotide sequence ID" value="XM_018856119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9T6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9T6"/>
    <dbReference type="GeneID" id="30029095"/>
    <dbReference type="OrthoDB" id="191918at2759"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072545">
        <property type="term" value="F:L-tyrosine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120284">
        <property type="term" value="F:tryptophan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.590.10:FF:000002">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.590.10">
        <property type="entry name" value="Chorismate mutase, AroQ class superfamily, eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008238">
        <property type="entry name" value="Chorismate_mutase_AroQ_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037039">
        <property type="entry name" value="CM_AroQ_sf_eucaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01802">
        <property type="entry name" value="CM_pl-yst"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21145:SF12">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017318">
        <property type="entry name" value="Chor_mut_AroQ_eu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51169">
        <property type="entry name" value="CHORISMATE_MUT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="9 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0827">Tyrosine biosynthesis</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="16">
        <location>
            <begin position="140"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR017318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA20776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="259" mass="30044" checksum="29DF2B015F8E0E12" modified="2016-10-05" version="1">MNFREPATVLDLNNIRASLVRMEETIVFGLIERSQFFSLPSVYQPNKYRIEGFNGSFMEWLLLQTEKVHSQVRRYEAPDETPFFPDQLLKPLLPSIEYPKILASYSDDVNVNKDILDFYVHKIVPQMACEQGDQNENSGSVSVCDVECLQALSRRIHFGKFVAEAKYKADPEMYKKLILARDVQGIEASITNQAVEDKILLRLKEKCQTYGTDPSLKYSQRPQAKVDPELIVHLYKQYVIPLTKKVEVEYLLRRLEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAP9</accession>
    <name>A0A1A0HAP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Iron-sulfur cluster assembly protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_25948</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711467.1">
        <property type="nucleotide sequence ID" value="XM_018855306.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAP9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAP9"/>
    <dbReference type="GeneID" id="30028282"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000005">
        <property type="entry name" value="Iron-sulfur cluster assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="159" mass="16884" checksum="6C84257CCA1A6830" modified="2016-10-05" version="1" fragment="single">ALSAARAVPAFSLAARPQHRGYHEKVLDHYSNPRNVGTMNKNDEDVGTGLVGAPACGDVMRLQIQVDEATGVIKDVKFKTFGCGSAIASSSYVTELVRGMTLEEAMTIKNTAIAKELSLPPVKLHCSMLAEDAIKSAVKDYKTKRANAVKQPTLGAAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAS1</accession>
    <name>A0A1A0HAS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_170243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711493.1">
        <property type="nucleotide sequence ID" value="XM_018854683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAS1"/>
    <dbReference type="GeneID" id="30027659"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="159" mass="18038" checksum="FFE40A9B41A61E13" modified="2016-10-05" version="1">MISDEIQSNDINVYGNPPNNIGTPSNHIGQNYIQMRTDGLNRGRSTSMGLERPRQAHNTSSRLPTHSRDESLLSTGLFFLPSIPLLAEHICIHRWCRQSAEKSEWHHQRAAKSRKPESRGLLQRPQAAFPSLLQCHRPWREKAPWPINPGPRPGARASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAX5</accession>
    <name>A0A1A0HAX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711542.1">
        <property type="nucleotide sequence ID" value="XM_018854035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAX5"/>
    <dbReference type="GeneID" id="30027011"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="139" mass="14964" checksum="585A910F115100AA" modified="2016-10-05" version="1">MNELIKYYDSGSDLEIPHSDEYDSDIDEETLLASSSDPDSESDSESENACEKCVGCVEESESESSSSESESGDAYGACLSQIKPACLKIKPLKDKVCLSLRNSEALPGACSVESKTQAPSLRDVGYGNIEESLMKSTEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB12</accession>
    <name>A0A1A0HB12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Calcium-transporting ATPase</fullName>
            <ecNumber evidence="16">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_31765</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the hydrolysis of ATP coupled with the transport of calcium.</text>
    </comment>
    <comment type="function">
        <text evidence="15">This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Has a role in the secretory pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18105"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 16">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="16"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711690.1">
        <property type="nucleotide sequence ID" value="XM_018855910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB12"/>
    <dbReference type="STRING" id="869754.A0A1A0HB12"/>
    <dbReference type="GeneID" id="30028886"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140613">
        <property type="term" value="F:P-type manganese transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000008">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000028">
        <property type="entry name" value="Calcium-transporting P-type ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006413">
        <property type="entry name" value="P-type_ATPase_IIA_PMR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01522">
        <property type="entry name" value="ATPase-IIA2_Ca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08282">
        <property type="entry name" value="Hydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 16" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 16" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="11 16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="302"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="719"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="792"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="824"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="859"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="17">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="928" mass="101961" checksum="C7DB900BA8CD175C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB31</accession>
    <name>A0A1A0HB31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein L10e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_78327</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711844.1">
        <property type="nucleotide sequence ID" value="XM_018858863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB31"/>
    <dbReference type="STRING" id="869754.A0A1A0HB31"/>
    <dbReference type="GeneID" id="30031839"/>
    <dbReference type="OrthoDB" id="10258869at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="220" mass="25241" checksum="0E00DDB3F7DED02B" modified="2016-10-05" version="1">MARRPARCYRYCKNKPYPKSRYNRAVPDAKIRIYDLGRKKATVDEFPLCVHLVSNELEQLSSEALEAARICANKYITKISGRDSFHLRVRVHPFHVLRINKMLSCAGADRLQQGMRGAWGKPHGLAARVNIGQILMSARTKDSNKAVVIEGLRRARYKFPGQQKIIISKKWGFTPLNRDEYIAKKNNGEVRDDGAYVKFLSRKGNLAENLAQFPDYKYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB88</accession>
    <name>A0A1A0HB88_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Tethering factor for nuclear proteasome STS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_40790</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ubiquitin-mediated protein degradation. Regulatory factor in the ubiquitin/proteasome pathway that controls the turnover of proteasome substrates. Targets proteasomes to the nucleus and facilitates the degradation of nuclear proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cut8/STS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711786.1">
        <property type="nucleotide sequence ID" value="XM_018857312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB88"/>
    <dbReference type="STRING" id="869754.A0A1A0HB88"/>
    <dbReference type="GeneID" id="30030288"/>
    <dbReference type="OrthoDB" id="10061064at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031144">
        <property type="term" value="P:proteasome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1590">
        <property type="entry name" value="Tethering factor for nuclear proteasome Cut8/Sts1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013868">
        <property type="entry name" value="Cut8/Sts1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038422">
        <property type="entry name" value="Cut8/Sts1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28032:SF1">
        <property type="entry name" value="FI02826P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08559">
        <property type="entry name" value="Cut8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="301" mass="33228" checksum="69D6B84BA5104F3A" modified="2016-10-05" version="1">MQDAFRPTKIVGKKRRCAEDEPGPAKQGQARRHVTPGAAARFKRLKPPGIAGLSLPVNRLIEVLDHASVLALLQTVMAHHPEIVPTVTKVAPKSSVADTIELIQQKAARIKAALPYRCDMESDYSYIRVKPHLTEFLACISDFVLDLLPPMDSPLHRACVVLDAIATAVHELPNFSNKEFQYTRSMAYEQLATLWLAVLNHHKSPEDPESAGEEACAASPSLEKSIDWLKTIEEMHLLQQVERHNDVSGGKFAALVDFVRAELDSFAQLNHGFSNSNTGSIFLDLISVDYSGFSISARTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBB2</accession>
    <name>A0A1A0HBB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31844</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711811.1">
        <property type="nucleotide sequence ID" value="XM_018855931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBB2"/>
    <dbReference type="GeneID" id="30028907"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="73" mass="8037" checksum="69652B2394A7BC40" modified="2016-10-05" version="1">MQPVGKQREFTSLEAFGTIAFASLMLLAAAGGLRKNIGKMSKGSNEKHSQSLKLERKPMLLYEDTQSTHTPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBE8</accession>
    <name>A0A1A0HBE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RCC1/BLIP-II protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_78329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711846.1">
        <property type="nucleotide sequence ID" value="XM_018858864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBE8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBE8"/>
    <dbReference type="GeneID" id="30031840"/>
    <dbReference type="OrthoDB" id="10256179at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053245">
        <property type="entry name" value="MitoProcess-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47563:SF1">
        <property type="entry name" value="PROTEIN FMP25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="RCC1" evidence="1">
        <location>
            <begin position="491"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="644" mass="69706" checksum="2123C2CAC75585C6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBQ4</accession>
    <name>A0A1A0HBQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711817.1">
        <property type="nucleotide sequence ID" value="XM_018854111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HBQ4"/>
    <dbReference type="GeneID" id="30027087"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038872">
        <property type="entry name" value="Put_GTT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807:SF1">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="283" mass="31895" checksum="7524D11A73DDC5A6" modified="2016-10-05" version="1">MRSVGAATSRKRKPQSLLPLYTTSTTSQDSANLSSPEAGVLLNFYREIETSSHSSIASLRAVLGLGKKLNIGFGETNFGTYQNPHYREARAVYIPISKTAACNFGELAVEAFFFLRFYVVLVPVKTNLSIHSFLRKRFSHLDSWIMPIPDLVALCTFSAISVFISWAVCSILIPLWISRSVGFSRKIYARPALAKTEFQNTKHGNYDPLVFALVKLAINCYIYMYYATFSLEIYGNFKLFRGLIALQLGFYNLHLLRMGVFPLISGAIITATSFYSRLRDKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBR9</accession>
    <name>A0A1A0HBR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">DUF663-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_180053</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711832.1">
        <property type="nucleotide sequence ID" value="XM_018854764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBR9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBR9"/>
    <dbReference type="GeneID" id="30027740"/>
    <dbReference type="OrthoDB" id="10260897at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="70"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="409"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="673"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="761"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1176"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="466"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="673"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="708"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="763"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1176"/>
            <end position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1221" mass="137019" checksum="0A9E58566D548B7F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBY2</accession>
    <name>A0A1A0HBY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_182790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711908.1">
        <property type="nucleotide sequence ID" value="XM_018854785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBY2"/>
    <dbReference type="GeneID" id="30027761"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291738" description="Secreted protein" evidence="1">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="11203" checksum="C5FE85A693C8F7F4" modified="2016-10-05" version="1">MNSPSVKIPILLIPPSIALWLHRISTLPAILYFVAAQRNVIICDVNDQESLGNRNIPCYSLPQTNEDTTYIRYEFSWRSRCLTTGKEAIKWKYICQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC54</accession>
    <name>A0A1A0HC54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_83143</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712075.1">
        <property type="nucleotide sequence ID" value="XM_018859226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC54"/>
    <dbReference type="GeneID" id="30032201"/>
    <dbReference type="OrthoDB" id="4093184at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="577" mass="62889" checksum="6EAD43F7B3386148" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC80</accession>
    <name>A0A1A0HC80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712008.1">
        <property type="nucleotide sequence ID" value="XM_018854142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC80"/>
    <dbReference type="GeneID" id="30027118"/>
    <dbReference type="OrthoDB" id="4093861at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="125" mass="13680" checksum="3313CB9FDAE70BBB" modified="2016-10-05" version="1">MEEEKIAIVAQPDSGAENGKTRGTTLKKLQGLVERDLLKERTMLDELGGPSQGGRPDHAGYDQDGFKVPVRKIPLDGQFSGPELKELVELSKDKGLLKDDVSVRATASNEISKHMEIAEPKREKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCT3</accession>
    <name>A0A1A0HCT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712212.1">
        <property type="nucleotide sequence ID" value="XM_018857309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCT3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCT3"/>
    <dbReference type="GeneID" id="30030285"/>
    <dbReference type="OrthoDB" id="3365616at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22952">
        <property type="entry name" value="ART10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="638" mass="71361" checksum="C2EFF2A9067E76D4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCX3</accession>
    <name>A0A1A0HCX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flavo protein-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_23869</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HFCD (homooligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712362.1">
        <property type="nucleotide sequence ID" value="XM_018855270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCX3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCX3"/>
    <dbReference type="GeneID" id="30028246"/>
    <dbReference type="OrthoDB" id="1532798at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF17">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE SUBUNIT SIS2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="3">
        <location>
            <begin position="190"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="381"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="404"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="434" mass="47647" checksum="6FB07C0DDAB75370" modified="2016-10-05" version="1" fragment="single">PSSPQATEIPPAVQAISNLRCPSPPPILTNSRSTSCSTPRLLLVSPGAPESFSDSHLSAAEITHGSPAVFSERATGSPALVVPGVGSSLGADVTTANIRDSALDVKLDYTQNPPSQSIHHKSPSVHAHFFVDETQKPLKSGNRSRSGSASASGSEYLAGPMKRDERLQLPSAEQNTNIDPRLPQDDNKLHILFGVCGAISTMKIKLIINKLYEIYSLDKIAIQLILTKASENFISSETLQHLESNKKVRIWRDSDEWTTWSSRSDPVLHIELRRWADMLVVCPLTANTLSKISLGLCDNLLTSVIRAWNSAYPILLAPAMVSNAYNAATTKRQLRLISEEMPWMEVLKPVEKVVGSYGDIGMGGMMDWNEIVNKIVMKNGGYPEEDEEEDCKSNDNDHAIIDDNNNDDDDDDDDDDDDDDDDDDDDDDDDDDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD93</accession>
    <name>A0A1A0HD93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_40062</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712363.1">
        <property type="nucleotide sequence ID" value="XM_018857223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD93"/>
    <dbReference type="STRING" id="869754.A0A1A0HD93"/>
    <dbReference type="GeneID" id="30030199"/>
    <dbReference type="OrthoDB" id="2449818at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000006">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000002">
        <property type="entry name" value="Ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.190.20:FF:000009">
        <property type="entry name" value="Ribosomal protein L10a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="217" mass="24504" checksum="064624B96E799F47" modified="2016-10-05" version="1">MSKITTSHVRENVKKLLEHSNETKKRNFLETVELQVGLKNYDPQRDKRFSGTLKLPQVPRPNMTLCIFGDAFDCDRAKSVGVDAMSVDDLKKLNKNKKLIKKLAKKYNAFIASEVLIKQVPRLLGPQLSKAGKFPTPVSHNDDLYAKVQDVKSTIKFQLKKVLCLAVAVGHVEMEEEVIANQIMMASNFLVSLLKKNWQNVGSLVIKSTMGPSFRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDF1</accession>
    <name>A0A1A0HDF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712605.1">
        <property type="nucleotide sequence ID" value="XM_018855763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDF1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDF1"/>
    <dbReference type="GeneID" id="30028739"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21615:SF2">
        <property type="entry name" value="CYCLIN N-TERMINAL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="59"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="56703" checksum="D2140B7E1AE2CACB" modified="2016-10-05" version="1">MTQTLMSPPEYRHQDSVPLIHDTYRGENVTVNQYKYLAALYESELRTHALVVDEYMDDVTEAMQEALERTKPLAQFLTHQPYLTPSIRFKLLDFGLKLLVRLKVLPFVFCRAVRLFDRYCLKAIVLLPHGQLLMATCLWIAAKIHGGNCHFANMHSERPPAEIRSVLDLGYGAGARFNGPTQRYRLPRVTELIRLCGSRCNYNSKMFHQMEIAIMLNTNYDAANASRPCDYEFRRIKQFVAYAACFLYETVGYSPTQVAKTTIDLINQTFMLLRRDPRFQVPNQCVDVNDCNPDYFASSMLRKLLVSAVAKSPAFLLDLFADKGPQQLFSLIVSPSSIPALDIPKQPPLLTQTYSMPPLVSSPSSAQFGNSPVTPREYKYERVEIARSVKRLAERPRVVASQGEVGSRPCRRNTPVLLTPTPNPANLHSHHYPSILPHSSTNYAMSNAYPSSGRSSKSPKESEIFDQERPKLGAATPASSENEYKDRGFADVPAPMFKRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDJ0</accession>
    <name>A0A1A0HDJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial fission 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_10897</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in mitochondrial fission.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The C-terminus is required for mitochondrial localization, while the N-terminus is necessary for mitochondrial fission.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FIS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712489.1">
        <property type="nucleotide sequence ID" value="XM_018853877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDJ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HDJ0"/>
    <dbReference type="GeneID" id="30026853"/>
    <dbReference type="OrthoDB" id="421154at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090141">
        <property type="term" value="P:positive regulation of mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12212">
        <property type="entry name" value="Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016543">
        <property type="entry name" value="Fis1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033745">
        <property type="entry name" value="Fis1_cytosol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028061">
        <property type="entry name" value="Fis1_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028058">
        <property type="entry name" value="Fis1_TPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247:SF0">
        <property type="entry name" value="MITOCHONDRIAL FISSION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13247">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14853">
        <property type="entry name" value="Fis1_TPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14852">
        <property type="entry name" value="Fis1_TPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008835">
        <property type="entry name" value="TPR_repeat_11_Fis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR008835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="16530" checksum="EA712607416CFBC6" modified="2016-10-05" version="1">MFRRSHPALEELNVAIAPEQLQILKNQLESESPPSPQSLFNYAWGLLKTPSLRSQKQALGLLAALYRDTPALRREALYYLALGSVKTGEFADARRYADALVEKEPDNAQFKALRQAIDDQVTQDGLIGLGIAGGVLAVGLGVLGAMIRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HED6</accession>
    <name>A0A1A0HED6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SUN-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_20034</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with EMP65.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SLP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712852.1">
        <property type="nucleotide sequence ID" value="XM_018854924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HED6"/>
    <dbReference type="STRING" id="869754.A0A1A0HED6"/>
    <dbReference type="GeneID" id="30027900"/>
    <dbReference type="OrthoDB" id="266334at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000099">
        <property type="entry name" value="Uncharacterized protein, isoform C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045120">
        <property type="entry name" value="Suco/Slp1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953">
        <property type="entry name" value="MEMBRANE PROTEIN CH1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12953:SF0">
        <property type="entry name" value="SUN DOMAIN-CONTAINING OSSIFICATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="13">
        <location>
            <begin position="43"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="17"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="428" mass="47760" checksum="8265C25BD2DBF85B" modified="2016-10-05" version="1" fragment="single">DDCKFLSFEEWKKLKEADTKAPLSKAQPGGPLPNQQAAPLPQNASSSPHKPEQQPVPEDQGRVYKDKFNYASGDCAATIVETNSDAKGAAAILTEVKDSYLLNKCLAASQFVVIELCQDILVGSVVMGNFELFSLMFRTVKFSVADRFPATQGWHDLGLFEAQNIRAIQTFDIASPLIWARYLKIEVASHYGNEFYCPISLVRVHGKTMMEEIKDSDRSTQPAPAETSRASPAQETPAAAFGWEDGAAECKVRGPFLALDQYLRDLNYSANYCDVPMMHTNVSHPALPETASTKPTQESIFQNIVKRLALLERNATLSLLYVEEQSKLLLDAFFNLENRQVRKFEHLLAHINETMYTQVHYLERAVGQIKSDSISMFRAQSAQALRSITGLRQETRAFAGEISFLRKLVVADTLIILALLVYVIGTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEN1</accession>
    <name>A0A1A0HEN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_11265</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712926.1">
        <property type="nucleotide sequence ID" value="XM_018853947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEN1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEN1"/>
    <dbReference type="GeneID" id="30026923"/>
    <dbReference type="OrthoDB" id="10263554at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032184">
        <property type="entry name" value="ATPase_V1_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="6">
        <location>
            <begin position="351"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR032184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="477" mass="53088" checksum="AA154B8D64FF19C6" modified="2016-10-05" version="1">MLHYAPLVLDSSSLLDSKKLIREKIVPWEGLARAGVISADQANLIKVLETQNHDSKRTTVLGSQQMYARTLLNLLSKLDVDARDDVLKCVLVLLNDLLVDAQAELFADALLELSSVDVALPYTPFVKHLDNTDPLIKTLSFYNVTVLLSKAAKVGNGAHSETLIKLFSVVCLKQFIGNTQDSNVQSIGVQLLQELLVNKPYRVVFQEHNLATNAKTLVRLIDTLAKQPNSVNLQLLYNTLLTVWILSFSPLLNKVLVHSFPSIVGSLLTIAKEGIKLKVVRVAVSTARNFVSFTVSLAEQFSVVKLLLFHDGLNVVKTIQTRKFALESSDEELAGDLAYLNEALSEVVTTKLTSLDAYLVELENPDLLSWASPTHKSEDFWRENAGKFRENSFALVRRMMDILAAEDTGSTAPARVILLNDLQFLIRNIGTDLVNFINTEKDSQYKMMVMGFLENSDGDNELKYEALRTIQYLVGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HES3</accession>
    <name>A0A1A0HES3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31497</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713120.1">
        <property type="nucleotide sequence ID" value="XM_018855865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HES3"/>
    <dbReference type="GeneID" id="30028841"/>
    <dbReference type="OrthoDB" id="4079529at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="84" mass="9367" checksum="71B9B4113F285E35" modified="2016-10-05" version="1">MNCPLCASELRKHLLSPSQAILSCPNEACAYPFNLPMDEVRRQGLTLRTSELEIMAQMGDKLARAQVDPRTARFMTKSDGDTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEZ4</accession>
    <name>A0A1A0HEZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_23921</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713067.1">
        <property type="nucleotide sequence ID" value="XM_018855273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HEZ4"/>
    <dbReference type="GeneID" id="30028249"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="145" mass="16735" checksum="737691D3055AD659" modified="2016-10-05" version="1" fragment="single">MGLILTDFFEPRSYDVEDTEVIVCDGCLAHLCLSSLVLSDQFWGLSGDAFLVDRLINILCEKQDLETSMRTGVYIINKIRCLQCSTKLGWHYKKSFKYSEYYKEGKYVIEKKYIRQIPNHSSTARLTEQAKLLKRRRSSASTTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFL6</accession>
    <name>A0A1A0HFL6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">DNA dependent ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_75892</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713168.1">
        <property type="nucleotide sequence ID" value="XM_018858439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFL6"/>
    <dbReference type="GeneID" id="30031415"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061635">
        <property type="term" value="P:regulation of protein complex stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18000">
        <property type="entry name" value="DEXHc_ERCC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000039">
        <property type="entry name" value="DNA repair protein Rhp26/Rad26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058951">
        <property type="entry name" value="WHD_Rad26_CSB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25875">
        <property type="entry name" value="WHD_Rad26_CSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="312"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="661"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="161"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1113" mass="124176" checksum="F82F0FB5DA1EEF65" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM7</accession>
    <name>A0A1A0HFM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_34440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713271.1">
        <property type="nucleotide sequence ID" value="XM_018856473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFM7"/>
    <dbReference type="GeneID" id="30029449"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="64" mass="7303" checksum="57E730F8BBFE307A" modified="2016-10-05" version="1" fragment="single">YTLISCFRLLWAPSGCPDNLTPFIVSDKCVFWHFNYTSGSSRIFSSACQKDPLNALHFCLQLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ8</accession>
    <name>A0A1A0HFQ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_75897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713174.1">
        <property type="nucleotide sequence ID" value="XM_018858441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFQ8"/>
    <dbReference type="GeneID" id="30031417"/>
    <dbReference type="OrthoDB" id="5286008at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070485">
        <property type="term" value="P:dehydro-D-arabinono-1,4-lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="21"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="349" mass="39455" checksum="D8BFA77D29A8395A" modified="2016-10-05" version="1">MPSKDFTLTKNPPYDLTDIPPLVIGGAVFNTQYSEDPESMPIRDILRVAFSKGLVALDTSPYYGKSEELIGNALSQISDEWPREKYFICTKAGRIRLNDFDYSRDLVRKSVLRSLQRLRTSYLDLVYLHDIEFVSEQQTYEALKELVALKKEGVIRNFGVTGYPVDYLLKIAMGSKNEYAGEIGPLDAILSYSNGCLQNTKLFEAHDRFMMEAGVRKVFNGSILSMSLLRSGKTHDFHPAPLALKGTCDQISQRLLEQDQVELAELATKYAIEKSLFTSKTDEENRLVWNKACSVVLGVSSVEELTSAIQGYEHVQANNNSNDHQLYERVQRELGPEHLNETWPSGIDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFW6</accession>
    <name>A0A1A0HFW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3533 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_75932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713229.1">
        <property type="nucleotide sequence ID" value="XM_018858450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFW6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFW6"/>
    <dbReference type="GeneID" id="30031426"/>
    <dbReference type="OrthoDB" id="2140105at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022703">
        <property type="entry name" value="DUF3533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053001">
        <property type="entry name" value="MNNG_permease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814:SF1">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12051">
        <property type="entry name" value="DUF3533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="2">
        <location>
            <begin position="72"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="56476" checksum="9CA256A8EC6BAD3A" modified="2016-10-05" version="1">MTDKSDSGSSINEGADAQLARAFEGHTNISPGGDAARSQSALQRVRSGFSFFNEKLSAQRKSLVIKTLLIYALMGACILGIFSIYWGSAYQRTSRFKNLRMLVVIEDDQTIDGTDPYIGDYLRQTLQTPEARELGNWLIQNNTAFQELALKNGHDIYAEVQRQVHHQRYWALIYVKPNATINLKNAIINGDTLYNVSYDSVVSFYETGRDMSAMILYVIPNLQTVGIMFGQQQANIMELILANVNTSEVFSSMDSVKVATSSLEFTYVDARPVTDEVLIAPLQVGLIYMIIVTFFGFNFFAEIHKGMIQAGVKKWHLVSYRVAASVSSFFFISLFFSLVTLAFQVDFTVAFGKSGFLVYWMTTFITMCAVGSMNEMVGMLCVMTYPPLLGFWLLFWVISNIAATFSPIALCPDFYRYTYAMPIHAAYEINKVIFFDTYKGAIGRNFGILVAWACIAITGMALVFFPFKNTLRKRAIAERRALEEQILASRAKDEEDRIRFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFW9</accession>
    <name>A0A1A0HFW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_37627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713535.1">
        <property type="nucleotide sequence ID" value="XM_018856900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFW9"/>
    <dbReference type="GeneID" id="30029876"/>
    <dbReference type="OrthoDB" id="4024240at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="530" mass="59253" checksum="3FD90124EA95190F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFX9</accession>
    <name>A0A1A0HFX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713545.1">
        <property type="nucleotide sequence ID" value="XM_018856869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFX9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFX9"/>
    <dbReference type="GeneID" id="30029845"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="263" mass="28315" checksum="B050D6D1A4574BE2" modified="2016-10-05" version="1">MSESFFDEAKPSFSHDSVSRIRTEGHGDEIVVLGNKRYYKHELMRAFGGTLQSERLAPFKEPKFGNAAALGLTAFATSAFVLGLSLAGAKGITVPNITVGLCFFYGGVVQAAAGIWELFLGNTFAGTVLCSFGMGFWMSFGAMNVEAFGIVAAYGTDTEMLSNAIGLYLVGWGIFTFLMLLCTLKTTLAFMGLFFTLDIGFFVLAGYYFTAEPKLLTTGGVFVTMAALCGWYCAFAGVATPENSYIVPFSFMLPVFGESKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG84</accession>
    <name>A0A1A0HG84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713497.1">
        <property type="nucleotide sequence ID" value="XM_018859358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG84"/>
    <dbReference type="STRING" id="869754.A0A1A0HG84"/>
    <dbReference type="GeneID" id="30032333"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000808">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="21703" checksum="DE41F286B79E6DFB" modified="2016-10-05" version="1">MPALKLVLLGDLSVGKSSLVNRFAVDSFDPHLPNTIGAAFISKEHVSKDRSVKFEVWDTAGQERYKSLTPMYYRNAKVALVCFDLSNTDLSFHRARYWCDQLAVLGPPDIDIVIVGTKLDLAETAVDVLVPEFCSDRGLRYVATSAKLGQGVLELFDSIVDGLDEEMFRQLELEPTEPEQENVLLFARARNSGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG97</accession>
    <name>A0A1A0HG97_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Las1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_93990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713364.1">
        <property type="nucleotide sequence ID" value="XM_018859379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG97"/>
    <dbReference type="STRING" id="869754.A0A1A0HG97"/>
    <dbReference type="GeneID" id="30032354"/>
    <dbReference type="OrthoDB" id="10263222at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="439" mass="50629" checksum="9A39A5A2C2E44C82" modified="2016-10-05" version="1">MTRTISITPYKNKSELLRVRELFYEDFNDSVSLKAAIDTVLYWQSRGKVPHAVNTTAWLVSAVLNDAILTERSKELRIRTERGMSDALPRNAEEYVLRNAYSMALVRFVNGILDPYQQGTYAVALLTLAKEIELPYFFVEVRHWATHEQLPSLEILRATSHRALEWLHIKFWSLLDASQNLKRTKFMEKEMHFIDIKLDVTAAFRAYRSCVKSKFTTESSRNNSSQDITVLEAQIHSIATSLSLDFHLARLLALKKALLKEASFSTIHETYGDLVAKLPPKFLLLLVHTLLLETHSNCSVHSIGSMVYLNTKEWFDLFLSLILGGPYPFRLHYHVYNNDEDVLQSLENYHRVLHYHNHIPCDFKTIKLTKKPDLKFAKAPLLEELLQVSSAPPGKQMKRNRNEDPEIQRVTNRSKAGRVFEKPSTWRILPFGISAMSNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGG1</accession>
    <name>A0A1A0HGG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_95463</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713448.1">
        <property type="nucleotide sequence ID" value="XM_018859407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGG1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGG1"/>
    <dbReference type="GeneID" id="30032382"/>
    <dbReference type="OrthoDB" id="5340910at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="301"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="366" mass="38868" checksum="E57EF4E3BCF470C3" modified="2016-10-05" version="1">MRLARDSLPVLRRDATVPTTVVVVTQVAATTTDSAASTSISEIDLTITILTTITAGAEPATSSSSTSSSAPTTAFSALSLAALAALSVSAPSAISSQSALGSISLTTTSTSLALATTVSLLLSTSLLSSTRLGLAIGIPIAAVCVFGLVVFLLIYLRKRMDLTPTETLAFKLPKTTLARSTNEKHAASSIYLSSDLEQGLEQTYNGRPSVPSGPDLGALQKPTFLKRISRLINVPESPLEFKSPLFLRRFHLLSNKYDAESDERQEKELPKSPATTPDELSLGHVAETGFPERKASSKRGVPELKYVVIKPYTRRLGDELTVSIGEQVSIVDHHSDGWAAVKLTETGEVGVIPLMCVKKFVGKNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGG4</accession>
    <name>A0A1A0HGG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_38233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713577.1">
        <property type="nucleotide sequence ID" value="XM_018856973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGG4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGG4"/>
    <dbReference type="GeneID" id="30029949"/>
    <dbReference type="OrthoDB" id="4026289at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56988">
        <property type="entry name" value="Anthrax protective antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508832" description="PA14 domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="2">
        <location>
            <begin position="48"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="210" mass="22151" checksum="B83C15DDAA0F231A" modified="2016-10-05" version="1" fragment="single">MRSSAPAKIQMLPIFLFLASTLAVTSGCTPATIGTTGFLARFYSYGLRDDSGWDADFFRSGYKTTLLQTVAGVTESNFQFSDQPDRVVIHNSIYGFDTTISNFSLELSAFFLAPDTGTYTFRLAADNGASLQFGSGQACCNDASGSVTGDFSIDTLGPAGGGGATDVNVETASFNLTAGVYYPIKIVMFNWQGNGGLDLKMTDSSGATIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGP9</accession>
    <name>A0A1A0HGP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Protoporphyrinogen oxidase</fullName>
            <ecNumber evidence="4 11">1.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_38549</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>protoporphyrinogen IX + 3 O2 = protoporphyrin IX + 3 H2O2</text>
            <dbReference type="Rhea" id="RHEA:25576"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="EC" id="1.3.3.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the protoporphyrinogen/coproporphyrinogen oxidase family. Protoporphyrinogen oxidase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.4" evidence="4 11"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713830.1">
        <property type="nucleotide sequence ID" value="XM_018857019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGP9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGP9"/>
    <dbReference type="GeneID" id="30029995"/>
    <dbReference type="OrthoDB" id="438553at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004729">
        <property type="term" value="F:oxygen-dependent protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004572">
        <property type="entry name" value="Protoporphyrinogen_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00562">
        <property type="entry name" value="proto_IX_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF3">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 11" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="12">
        <location>
            <begin position="17"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="528" mass="58406" checksum="6CA07604C61BF99E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGZ9</accession>
    <name>A0A1A0HGZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_64177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713935.1">
        <property type="nucleotide sequence ID" value="XM_018858341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGZ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGZ9"/>
    <dbReference type="GeneID" id="30031317"/>
    <dbReference type="OrthoDB" id="2573163at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF21">
        <property type="entry name" value="KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="614"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="631"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="640" mass="72353" checksum="EB286769590F560D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH09</accession>
    <name>A0A1A0HH09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Snf7-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_14427</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713761.1">
        <property type="nucleotide sequence ID" value="XM_018854503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH09"/>
    <dbReference type="STRING" id="869754.A0A1A0HH09"/>
    <dbReference type="GeneID" id="30027479"/>
    <dbReference type="OrthoDB" id="441172at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF5">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18897" checksum="97DC4802AEE7762F" modified="2016-10-05" version="1" fragment="single">MGNQPSQPKITAQEKAIFQLKRQRDNLKQYLKRINVVIERQTALAKQAVQDGNMDKAKIYLRLKKHQQSLINSTYDQLETLENLIGTIEFKLIEKDVLYGLSQGNEVLKKLNSEMSVDKIDRVMDDLEEERVKVDEVMDILGLGLTNAEEHEVDDELEALELEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH28</accession>
    <name>A0A1A0HH28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_29709</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713663.1">
        <property type="nucleotide sequence ID" value="XM_018855463.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HH28"/>
    <dbReference type="GeneID" id="30028439"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.120:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="323"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1309"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1336"/>
            <end position="1346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1389"/>
            <end position="1411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA23182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1648" mass="182521" checksum="C2E1D2C78D793BA7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH56</accession>
    <name>A0A1A0HH56_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="1 9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_35147</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="1 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713693.1">
        <property type="nucleotide sequence ID" value="XM_018856575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH56"/>
    <dbReference type="STRING" id="869754.A0A1A0HH56"/>
    <dbReference type="GeneID" id="30029551"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071507">
        <property type="term" value="P:pheromone response MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010526">
        <property type="term" value="P:transposable element silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000098">
        <property type="entry name" value="Mitogen-activated protein kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="14"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="367" mass="42732" checksum="ADB690E2147F2AD9" modified="2016-10-05" version="1">MSSRKVVFNISRSYETKQVLGEGAYGVVASAVHKPTGLTVAIKKIEPFSKTIVCLRTIRELHLLNFFRNHENIIGFYDVQRPKDFESFNEVYLIQEYMPTDLHKLNHSLDILDKHIQYFIYQILRGLKTIHSANVIHRDLKPLNVLVNYNCDVKICDFGLARIMGSTMLPLESKLTEYVATRWYRAPEIMLLQSMYTTAVDLWSVGCMMAELYLGYPLFPGKDYKHQLLLIFQYLGTPEGEDLECVKLPRARDYIQLLPRQRAVDVFLYFHNHPRRIQRFGTATINPLGLDLMTRLLTFDPEKRITASEALKHPYLEQYHDPSDEPITKVKLEPDYLDQKSKSDLSIDALKTYLYDAILEMTRSRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH75</accession>
    <name>A0A1A0HH75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713831.1">
        <property type="nucleotide sequence ID" value="XM_018859425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH75"/>
    <dbReference type="STRING" id="869754.A0A1A0HH75"/>
    <dbReference type="GeneID" id="30032400"/>
    <dbReference type="OrthoDB" id="277802at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13893">
        <property type="entry name" value="RRM_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="25"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="115" mass="12778" checksum="9694A3D8FF7F5D59" modified="2016-10-05" version="1">MGAVSKRTADAPNRPAKRLKLEVQQTLYIQNLNDKINRALLKHSLFMIFSTYGEVLEINMKMKGQAHIVLDSCESASHALRSLQATNILGKAIHIDYAKKKSVSIEAAEKAIAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH98</accession>
    <name>A0A1A0HH98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TIP41-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_15636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TIP41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713733.1">
        <property type="nucleotide sequence ID" value="XM_018854614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH98"/>
    <dbReference type="STRING" id="869754.A0A1A0HH98"/>
    <dbReference type="GeneID" id="30027590"/>
    <dbReference type="OrthoDB" id="10253878at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051330">
        <property type="entry name" value="Phosphatase_reg/MetRdx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007303">
        <property type="entry name" value="TIP41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021">
        <property type="entry name" value="GAF/PUTATIVE CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21021:SF16">
        <property type="entry name" value="TIP41-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04176">
        <property type="entry name" value="TIP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="311" mass="34916" checksum="99D05210081EC868" modified="2016-10-05" version="1" fragment="single">TAQVSVPATSHTGPCTNPRCAHCGTVVIPSPKATLPLREQPSITVNDWSIYTSKGPICLSEELDHLNDTRFDFPLPEMIFGNNRVLLVNEKTGARIDFNTLDALDSLAPTADFKVAYYKEWLSTRPKAKTSDTGSVATADGSFITMGSETPSPSPELQSVKPYDWTYSTNYRGTLSRCTFTKTAQDIPLEKLVRPDPILFYDESILFEDELGDNGISMLSTKIRVMHSCLLLLCRFSMRVDDVAFRIRDTRVYIDLETNVVLREYKTQQALYDELLGKVSGRSTDPKKLLRDSNWVSQNTPVVLREVEVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHH7</accession>
    <name>A0A1A0HHH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_29832</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713813.1">
        <property type="nucleotide sequence ID" value="XM_018855491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHH7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHH7"/>
    <dbReference type="GeneID" id="30028467"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF12">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="8"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="101"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="197"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="30901" checksum="A4B44C7A758041F2" modified="2016-10-05" version="1">MSVDESFSRKAKDVAAGFIGGATQVLIGQPADLVKIRLQTSTTTSSADIIKQVLTKEGPLAFYKGTLAPLFGVGACVSLQFYGFHETKRQLLKNSGESQLLLWPQTYIAGAMAGVVNTPVTAPVEQLRILSQSSTKSTSLKDTVKKIYGAHGFAGLYRGTTITLIREVQAYGVWFLTYESLIQGLINQRGYKLRGDISTIELLGCGALAGNALWLSSYPLDVIKSNIQSDKFGADSKYDGKISSATRAIWAANGMRGFWKGIVPCLLRAVPCSAGTFAAVETALRIMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHI3</accession>
    <name>A0A1A0HHI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713947.1">
        <property type="nucleotide sequence ID" value="XM_018859449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHI3"/>
    <dbReference type="GeneID" id="30032424"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="108" mass="12319" checksum="C3A56C71FC084D39" modified="2016-10-05" version="1">MAHIESTQFYNTKCGATEVSAMRVMRAEGDEEGHQSRTRIEVRRQQETKPKQQTTGSEQIQPEKTRPLQKQQKTKTQAPRIGLGVSLWKNTVSIPTNFAVKPSNRPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHJ9</accession>
    <name>A0A1A0HHJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADH dehydrogenase [ubiquinone] iron-sulfur protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex I-15 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase 15 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_34025</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Mammalian complex I is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the complex I NDUFS5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713961.1">
        <property type="nucleotide sequence ID" value="XM_018856431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHJ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHJ9"/>
    <dbReference type="GeneID" id="30029407"/>
    <dbReference type="OrthoDB" id="9992197at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd24141">
        <property type="entry name" value="NDUFS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019342">
        <property type="entry name" value="NADH_UbQ_OxRdtase_FeS-su5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15224:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10200">
        <property type="entry name" value="Ndufs5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="13"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR619342-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA23480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="92" mass="10420" checksum="D935393F7A72733B" modified="2016-10-05" version="1">MQPYSRSGGTRRCFYEFQSLVACYTAADTATKKECTPFFDDYSECLHGTKEREKARLMLQQLKANEMSGEGVKAADLYKNSGAVYEKLDLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHM5</accession>
    <name>A0A1A0HHM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_76510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713987.1">
        <property type="nucleotide sequence ID" value="XM_018858541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHM5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHM5"/>
    <dbReference type="GeneID" id="30031517"/>
    <dbReference type="OrthoDB" id="64915at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004104">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051450">
        <property type="entry name" value="Gfo/Idh/MocA_Oxidoreductases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43377:SF1">
        <property type="entry name" value="BILIVERDIN REDUCTASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02894">
        <property type="entry name" value="GFO_IDH_MocA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase C-terminal" evidence="2">
        <location>
            <begin position="143"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="418" mass="46004" checksum="2CC0E25155E286BC" modified="2016-10-05" version="1">MIPVSVVVIGAGLIGPRHAELVLKNSNCSLFAIVDRSAKGPSIASSCGTLHFQSIHDMLEFCDNHDANYPDAAVVCTPNDSHAEITVVLAEKGIHVLVEKPMASSAVDCIKMIDVCEKNNVKLLIGHHRRFNPYISATKSHMHRIGSPVAVQGVWALKKHQAYFDEKAWRRSRFLGGGAILINLIHDLDLLQYLLGPVIRVYAELMPRQRQAEGKDNHVDEGAVLTLRFANGCCGTFLCSDNVTSPYSFEAGTGENPLIPMHPDVAGFYRIFGSSGTLSVPDLTLYHQDNFSLRENSWWNPIQSESLGGVYQENDCMYVDDFSAITPPSSFEEKEANFGPATQKLPKPFDLQLGHFINLITGAETDVKCSGYDALQSMLCIEAVTRSINQGIAQTIQTAEELRAGFQGHESHQQPFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHS1</accession>
    <name>A0A1A0HHS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Polyprenal reductase</fullName>
            <ecNumber evidence="5">1.3.1.94</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76546</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Plays a key role in early steps of protein N-linked glycosylation by being involved in the conversion of polyprenol into dolichol. Acts as a polyprenal reductase that mediates the reduction of polyprenal into dolichal in a NADP-dependent mechanism. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a di-trans,poly-cis-dolichal + NADP(+) = a di-trans,poly-cis-polyprenal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80727"/>
            <dbReference type="Rhea" id="RHEA-COMP:19536"/>
            <dbReference type="Rhea" id="RHEA-COMP:19537"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:231623"/>
            <dbReference type="ChEBI" id="CHEBI:231637"/>
            <dbReference type="EC" id="1.3.1.94"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:80729"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the steroid 5-alpha reductase family. Polyprenal reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.94" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714032.1">
        <property type="nucleotide sequence ID" value="XM_018858550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHS1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHS1"/>
    <dbReference type="GeneID" id="30031526"/>
    <dbReference type="OrthoDB" id="541710at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003865">
        <property type="term" value="F:3-oxo-5-alpha-steroid 4-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160198">
        <property type="term" value="F:polyprenal reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102389">
        <property type="term" value="F:polyprenol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016095">
        <property type="term" value="P:polyprenol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039698">
        <property type="entry name" value="Dfg10/SRD5A3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624">
        <property type="entry name" value="DFG10 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14624:SF0">
        <property type="entry name" value="POLYPRENOL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="6">
        <location>
            <begin position="170"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="275" mass="31222" checksum="EC1F1BDCB91C501D" modified="2016-10-05" version="1">MIVAHAISASFAVLSVFLLALIQFNDLSVLVYYGKVAQGRLHQNLSPLATLFNGLAKLTVPKAWFAHFYILYLALMWSQVVLYPKSILENGKYTLVWLFLTCQASRRLYESFNVSRWSPASRMNVSHYLMGMLFYVGVSAACLLGLLSQDPLEEQKFSLLHKAWVGGLGLLFLLSQIDQYRNHKYLASLVKYSAPKNGVFCVVSSAHYLDEIVAYFCVVLLVFTRGQIVAEDWSLLSIWLFIVINLSISACETHKYYKQKFDDYSVRFALIPGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHU0</accession>
    <name>A0A1A0HHU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-aminoacid aminotransferase-like PLP-dependent enzyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_105862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713928.1">
        <property type="nucleotide sequence ID" value="XM_018853807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHU0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHU0"/>
    <dbReference type="GeneID" id="30026783"/>
    <dbReference type="OrthoDB" id="5288718at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="32137" checksum="3D75912E27F5592D" modified="2016-10-05" version="1">MSAEPEPELPTYEEFISSLGKKKQFTIDVPFELLSTIRYDPLLSKTPPRSAKEVKKENFFLLPEHIERIKFTLEFFGRLDDKSFKLNNAQFTIDESLMLDKLIDALEHSEVNIRKPLKIRLLINKAGDAKIELYETAERKSLLDGLIDDFPDSKRYDVYVDKTPVLASPFTSFKTTHRDIYTEARTRALPGKSAREEVIVINTTSEVMEGSITNIAIKSKQGIWITPKLTSGCLCGVTRHFLLRKNFIQEGDISVEMLRTGHDVMLMNGVMGCVRGTIKGFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI28</accession>
    <name>A0A1A0HI28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">1-phosphatidylinositol-4-phosphate 5-kinase</fullName>
            <ecNumber evidence="2">2.7.1.68</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">1-phosphatidylinositol 4-phosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphosphoinositide kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">PIP5K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_35886</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14425"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.68"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.68" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713979.1">
        <property type="nucleotide sequence ID" value="XM_018856666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI28"/>
    <dbReference type="STRING" id="869754.A0A1A0HI28"/>
    <dbReference type="GeneID" id="30029642"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031321">
        <property type="term" value="P:ascospore-type prospore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17303">
        <property type="entry name" value="PIPKc_PIP5K_yeast_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.800.10:FF:000009">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 5-kinase its3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="66"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="454" mass="52352" checksum="5C088783B196CCCE" modified="2016-10-05" version="1">MSTILRPSSKDKNPSVAGDHHQGRKHSEPESELTRMKSTMVTKNIQRKKDIVDDMTHIVGNKITEGHQNFVMAYNMLTGIRVAVSRCSGVMRKLNDADFRATKKLSFDMDGTELTPSSKYDFKFKDYSPEVFRELRSLFGIDPADYLMSITGKYILSELGSSGKSGSFFYYSRDYRFIIKTIHHSEHKQLRRILHEYYHHVKNNPNTMVSQFYGLHRLKVKGGIKRVHFLVMNNLFPPHRDIHVKYDLKGSTWGRYTKIPETSGNEDLSSHTLKDLNWLQRKDRIKFGPEKKRIFLEQLEADVKLLKKINVMDYSLLLGIHDVKKGNSETVAKLTVFDPKSNLKAEVFKTNPRGIDRQNDLPSDVFPGRSKYIFYGHNGGIRATNESNEPVSEIYYLGIIDCLTNYSFKKKLETAWRSITQSRSTISAVPAREYGDRFIQFIKDGISDVKVKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI30</accession>
    <name>A0A1A0HI30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Mannose-6-phosphate isomerase</fullName>
            <ecNumber evidence="5 12">5.3.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_107331</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>D-mannose 6-phosphate = D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:12356"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="5.3.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the mannose-6-phosphate isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.8" evidence="5 12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714018.1">
        <property type="nucleotide sequence ID" value="XM_018853839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI30"/>
    <dbReference type="STRING" id="869754.A0A1A0HI30"/>
    <dbReference type="GeneID" id="30026815"/>
    <dbReference type="OrthoDB" id="6605218at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00423"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004476">
        <property type="term" value="F:mannose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07011">
        <property type="entry name" value="cupin_PMI_type_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.441.10:FF:000001">
        <property type="entry name" value="Mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.441.10">
        <property type="entry name" value="Phosphomannose Isomerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001250">
        <property type="entry name" value="Man6P_Isoase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016305">
        <property type="entry name" value="Mannose-6-P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018050">
        <property type="entry name" value="Pmannose_isomerase-type1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046456">
        <property type="entry name" value="PMI_typeI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046457">
        <property type="entry name" value="PMI_typeI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046458">
        <property type="entry name" value="PMI_typeI_hel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00218">
        <property type="entry name" value="manA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10309:SF0">
        <property type="entry name" value="MANNOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01238">
        <property type="entry name" value="PMI_typeI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20511">
        <property type="entry name" value="PMI_typeI_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20512">
        <property type="entry name" value="PMI_typeI_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001480">
        <property type="entry name" value="Mannose-6-phosphate_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00714">
        <property type="entry name" value="MAN6PISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00965">
        <property type="entry name" value="PMI_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00966">
        <property type="entry name" value="PMI_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 18" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphomannose isomerase type I catalytic" evidence="16">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I helical insertion" evidence="17">
        <location>
            <begin position="172"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphomannose isomerase type I C-terminal" evidence="15">
        <location>
            <begin position="349"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001480-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF20512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA23537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="440" mass="47210" checksum="8351E4609DF1F696" modified="2016-10-05" version="1">MSPLFRIDCGYQNYDWGKIGSASAVARFAAKSNADTAIDENKPYAELWMGTHPSVPSTIVDDKALQGKVLRDVVGGNAAVYLHPSVVRKFGSDKELPFLFKVLSIEKVLSIQAHPDKALAAQLHAADPKNYPDDNHKPEMAIAVTDFEGFCGFKPLRQIAATLQAVPEFRDIVGAETAADFSSNIQPDAAAGSAADKANRALLQKVFGNVMTTSDADVAAKAALLVSRAQREPQVFDALAPGLADLVLRLNKQFPNDIGLFCGCMMLNHVRLAAGEAMFLQAKDPHAYISGDIIECMAASDNVVRAGFTPKFKDVANLVDMLTYSYDPVDKQKMPLLPFPRSHGDAPTSVLYDPPIAEFAVLQTIFAATPGQTQYFDGFAGPSIVIATNGTGLIKVKGSDSALPVETGYVFFIAPGTEIELVSSSTAEPLTTYRAFVEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI48</accession>
    <name>A0A1A0HI48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_107155</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713999.1">
        <property type="nucleotide sequence ID" value="XM_018853830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI48"/>
    <dbReference type="GeneID" id="30026806"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="1">
        <location>
            <begin position="109"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="231" mass="26879" checksum="880528E7F86A09D9" modified="2016-10-05" version="1">MLKKCMETLQHETQKLSGALQVIMNSMKIEHESPEKILNDSPAQSNNRPFQLFSPIKLKFDSSEQANDKYDHHSDDQYSILRKKIKINPTSKYYSIPKTVEDCFKTPEFRMLPKDIPVSTLLKEFQIPIPGRPSLLERESKYGAKWRYDQLALWKFKKTFFNFVTKTSEELNFAPSVVAQIMDTIAQQQGLRGMASMRVKIQDSLLQKEMLREISNILTDQQETNNNNEVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI66</accession>
    <name>A0A1A0HI66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_114432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714340.1">
        <property type="nucleotide sequence ID" value="XM_018853988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI66"/>
    <dbReference type="GeneID" id="30026964"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="99" mass="11581" checksum="D838F219431554A1" modified="2016-10-05" version="1">MFSTSAAVHKRNPLIPVWSWLLSGSQRAIAYRYKKNFRNTAMQFTTCRLFPKIQSANCFCVLYKCSIRILILPRAWNNLQSIAKPRRQNLAINFVFSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HID3</accession>
    <name>A0A1A0HID3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor IIIC subunit 5 HTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9994</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714246.1">
        <property type="nucleotide sequence ID" value="XM_018859503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HID3"/>
    <dbReference type="STRING" id="869754.A0A1A0HID3"/>
    <dbReference type="GeneID" id="30032478"/>
    <dbReference type="OrthoDB" id="5598268at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001002">
        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001003">
        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.160">
        <property type="entry name" value="TFIIIC, subcomplex tauA, subunit Sfc1, barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019136">
        <property type="entry name" value="TF_IIIC_su-5_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040454">
        <property type="entry name" value="TF_IIIC_Tfc1/Sfc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041499">
        <property type="entry name" value="Tfc1/Sfc1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042536">
        <property type="entry name" value="TFIIIC_tauA_Sfc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230:SF5">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIIC, POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09734">
        <property type="entry name" value="Tau95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17682">
        <property type="entry name" value="Tau95_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Transcription factor IIIC subunit Tfc1/Sfc1 triple barrel" evidence="7">
        <location>
            <begin position="18"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC subunit 5 HTH" evidence="6">
        <location>
            <begin position="202"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="491"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="498"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="527"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="587" mass="67436" checksum="962290F11E588107" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIE1</accession>
    <name>A0A1A0HIE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquinone biosynthesis O-methyltransferase, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinol 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">3-demethylubiquinone-6 3-O-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Hexaprenyldihydroxybenzoate methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Polyprenyldihydroxybenzoate methyltransferase</fullName>
            <shortName evidence="5">DHHB methyltransferase</shortName>
            <shortName evidence="5">DHHB-MT</shortName>
            <shortName evidence="5">DHHB-MTase</shortName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
            <ecNumber evidence="5">2.1.1.114</ecNumber>
            <ecNumber evidence="5">2.1.1.64</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COQ3</name>
        <name evidence="6" type="ORF">METBIDRAFT_37051</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">O-methyltransferase required for two non-consecutive steps during ubiquinone biosynthesis. Catalyzes the 2 O-methylation of 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid into 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoic acid. Also catalyzes the last step of ubiquinone biosynthesis by mediating methylation of 3-demethylubiquinone into ubiquinone. Also able to mediate the methylation of 3-demethylubiquinol into ubiquinol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-methionine = a 4-hydroxy-3-methoxy-5-(all-trans-polyprenyl)benzoate + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="Rhea" id="RHEA-COMP:10931"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:84443"/>
            <dbReference type="EC" id="2.1.1.114"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3-demethylubiquinone + S-adenosyl-L-methionine = a ubiquinone + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:81215"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EC" id="2.1.1.114" evidence="5"/>
    <dbReference type="EC" id="2.1.1.64" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714133.1">
        <property type="nucleotide sequence ID" value="XM_018856815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIE1"/>
    <dbReference type="STRING" id="869754.A0A1A0HIE1"/>
    <dbReference type="GeneID" id="30029791"/>
    <dbReference type="OrthoDB" id="3265906at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061542">
        <property type="term" value="F:3-demethylubiquinol 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120537">
        <property type="term" value="F:3-demethylubiquinone 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010420">
        <property type="term" value="F:polyprenyldihydroxybenzoate methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43464:SF19">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS O-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 5" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="316" mass="35951" checksum="3B0B784D421B2E62" modified="2016-10-05" version="1">MIKQALQRTRPVILCRAFSISCSLRHEVNKATSASKDEVTHFNALASSWWDVDGPQRILHKMNLLRMDYINEVIKNNVKLNADTTSADEEVYIAPYNLDLLPKSIKDRILEEQDEKRAEIFAEKPLKVLDVGCGGGILSESLARLNYVDSVKGIDLSADVLEAAKVHMKKDPDLTDVLLYEYQAVEELPKGERFDIITVFEMLEHVSYPAQILSEVFDRLETGGFVFLSTINRDFVSWFTTIFMGEHVLGIVPVGTHSVKKYINESEIRQWLHSSEYKKNFKIVDSRGCVYLPACGWKFTSNAEMGNYFMAIQKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ04</accession>
    <name>A0A1A0HJ04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_115949</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714476.1">
        <property type="nucleotide sequence ID" value="XM_018854026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ04"/>
    <dbReference type="GeneID" id="30027002"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="156" mass="16853" checksum="64D22DE251E36874" modified="2016-10-05" version="1">MHVSGRSAAPASAPDISQPRDPRFLRRATYGACCGALPALAHGRFSEPKRRFALKPPAVTCLGPLPLGFLVPPRLAPNLANITWRPGKYARVAPSVGLSVRSISRCAGPGYFHSRSQMHWGRSPYRGCKLIAARDQGTARCVQARAHPARADALYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ05</accession>
    <name>A0A1A0HJ05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 74</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_34289</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Mediates the cis and medial Golgi localization of mannosyltransferases through direct binding of their cytosolic domains. Involved in vacuolar protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714620.1">
        <property type="nucleotide sequence ID" value="XM_018856463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ05"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ05"/>
    <dbReference type="GeneID" id="30029439"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048194">
        <property type="term" value="P:Golgi vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060304">
        <property type="term" value="P:regulation of phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3630.10:FF:000002">
        <property type="entry name" value="Vacuolar sorting-associated 74 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704:SF2">
        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3 HOMOLOG SAURON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12704">
        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="336" mass="37663" checksum="BB68FDA86B1422C1" modified="2016-10-05" version="1">MSLSVLQKRRGGKPAQAASAASRSLFDEDDFASDGHKIGIDARDIAQDHTVKIPFLTLMEEVLLMGLKDKEGYLSFWNDNISYALRGCILLELAFRGKITLVNDPARRRFDLCDRLVEVVNTELTGEVLLDEALKIMKNDEGHLSVSNWIDLLSGETWNLMKINYQLKQVRERLAKGLVDKGILRTERKNFFLFDMATHPVADKMSKENIRNRVLSFLTLRTIDLDALSNDQFAADTSYRHLRTVALVCGAYAANVLENVLLALNYDARDQAFARADELLNEYSEFPFCAKGPLSPLGLGLNLAEEVGKETEQTSELTLELIAAVVSVFARMDSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ18</accession>
    <name>A0A1A0HJ18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GDS1 winged helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36951</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714477.1">
        <property type="nucleotide sequence ID" value="XM_018856806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ18"/>
    <dbReference type="GeneID" id="30029782"/>
    <dbReference type="OrthoDB" id="4090479at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057511">
        <property type="entry name" value="WH_GDS1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25318">
        <property type="entry name" value="WHD_GDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDS1 winged helix" evidence="2">
        <location>
            <begin position="159"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="513" mass="54447" checksum="08B29340BA017AED" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ50</accession>
    <name>A0A1A0HJ50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glycine--tRNA ligase</fullName>
            <ecNumber evidence="4">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_30378</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 ATP + H(+) = P(1),P(4)-bis(5'-adenosyl) tetraphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34935"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714507.1">
        <property type="nucleotide sequence ID" value="XM_018855627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ50"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ50"/>
    <dbReference type="GeneID" id="30028603"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00858">
        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.200:FF:000001">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000004">
        <property type="entry name" value="Glycine--tRNA ligase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000158">
        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.230:FF:000002">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000010">
        <property type="entry name" value="Glycyl-tRNA synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="193"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA24026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="658" mass="73880" checksum="7F8A452D5FA6EEB1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJL9</accession>
    <name>A0A1A0HJL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_77033</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714672.1">
        <property type="nucleotide sequence ID" value="XM_018858626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJL9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJL9"/>
    <dbReference type="GeneID" id="30031602"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140713">
        <property type="term" value="F:histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140719">
        <property type="term" value="P:constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007063">
        <property type="term" value="P:regulation of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000002">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.210:FF:000001">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000017">
        <property type="entry name" value="FACT complex subunit SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048969">
        <property type="entry name" value="FACT_SPT16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21091">
        <property type="entry name" value="SPT16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="12">
        <location>
            <begin position="6"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="13">
        <location>
            <begin position="557"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="833"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="952"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="454"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="956"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1003"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1017"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA24191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1031" mass="116336" checksum="0EABEB9518441D40" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJN1</accession>
    <name>A0A1A0HJN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714706.1">
        <property type="nucleotide sequence ID" value="XM_018855659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJN1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJN1"/>
    <dbReference type="GeneID" id="30028635"/>
    <dbReference type="OrthoDB" id="5598057at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd13311">
        <property type="entry name" value="PH_Slm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000328">
        <property type="entry name" value="Phosphatidylinositol 4,5-bisphosphate-binding protein SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043453">
        <property type="entry name" value="Slm1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF16">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE-BINDING PROTEIN SLM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="351"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="478"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="714"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="754"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="529"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="575"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="590"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="725"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="814" mass="91034" checksum="1F88F0B28DBD7A57" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX0</accession>
    <name>A0A1A0HJX0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714663.1">
        <property type="nucleotide sequence ID" value="XM_018855651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX0"/>
    <dbReference type="GeneID" id="30028627"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="85" mass="10017" checksum="0CD75A4343D0DFEE" modified="2016-10-05" version="1">MPIKYSLYPSNIHYTYRTLPVSILHHQEPSNIYLYIEHDQYPSNMTSTHQTLTTTIQRLLQPPNTHYIYRTPPVPTASPLLKPPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK96</accession>
    <name>A0A1A0HK96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_34451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714907.1">
        <property type="nucleotide sequence ID" value="XM_018856474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK96"/>
    <dbReference type="STRING" id="869754.A0A1A0HK96"/>
    <dbReference type="GeneID" id="30029450"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="3">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="528" mass="57471" checksum="4F7FA6C5178F1B78" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKZ1</accession>
    <name>A0A1A0HKZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DUF1014-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37905</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC124 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715043.1">
        <property type="nucleotide sequence ID" value="XM_018856927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKZ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKZ1"/>
    <dbReference type="GeneID" id="30029903"/>
    <dbReference type="OrthoDB" id="76412at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010422">
        <property type="entry name" value="Ccdc124/Oxs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054414">
        <property type="entry name" value="Ccdc124/Oxs1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054413">
        <property type="entry name" value="LSO1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21680:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06244">
        <property type="entry name" value="Ccdc124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22048">
        <property type="entry name" value="LSO1_2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LSO1/LSO2" evidence="5">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Coiled-coil" evidence="4">
        <location>
            <begin position="115"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="224" mass="24670" checksum="A8BB48450FE30272" modified="2016-10-05" version="1">MAKKGGENAKKVAGNAKKAEAANKKRTAAEQAAEAAEASQWEDGAKKGGKKKEDAQFKKEEAARKKAERDLLLAAEEASLPSKPQSSKQRGAAKVAAKRTGKIDDFLDFNSSTPELSASGLDDALSALALTGKDAGVGAKDIDRHPERRVKAAFAAYEERRLPEMRKESPGLRLQQMKQLIFKEFQKSDENPMNQETNVLYNASRDDVEYKKAEVRGQREKKFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GWP8</accession>
    <name>A0A1A0GWP8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_14069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000015">
        <property type="protein sequence ID" value="OBA16125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709235.1">
        <property type="nucleotide sequence ID" value="XM_018854479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GWP8"/>
    <dbReference type="GeneID" id="30027455"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="140" mass="15852" checksum="A68DAECAC26F6482" modified="2016-10-05" version="1">MLAQKMHVKIPKELDSESFHMMIAIITPENEVQVFNFGTSWAGLFRDGNLIAHTVLQKRQDSSYQYGHSAKPAETIWDVSEHKWQAQPGDLVLSDEPFLKEKDFDLYAMGIVKAARIAAGEQPNNDMFASVIRIGEERDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H255</accession>
    <name>A0A1A0H255_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_33783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709433.1">
        <property type="nucleotide sequence ID" value="XM_018856397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H255"/>
    <dbReference type="GeneID" id="30029373"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="57" mass="6393" checksum="BE09E8C83E7E8A35" modified="2016-10-05" version="1">MAAPQFSILSPNIPLSVALRSLNNVGRTTRNVFTVTWPQPMIIYDILVRNPEISLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4H2</accession>
    <name>A0A1A0H4H2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_223745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709509.1">
        <property type="nucleotide sequence ID" value="XM_018855162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4H2"/>
    <dbReference type="GeneID" id="30028138"/>
    <dbReference type="OrthoDB" id="4018768at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="586" mass="66034" checksum="F6582B5294EFBD25" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4U3</accession>
    <name>A0A1A0H4U3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_33673</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709591.1">
        <property type="nucleotide sequence ID" value="XM_018856376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4U3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4U3"/>
    <dbReference type="GeneID" id="30029352"/>
    <dbReference type="OrthoDB" id="3477330at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="509"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="808" mass="93507" checksum="1FCC23070F426363" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H522</accession>
    <name>A0A1A0H522_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_13450</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709668.1">
        <property type="nucleotide sequence ID" value="XM_018854365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H522"/>
    <dbReference type="STRING" id="869754.A0A1A0H522"/>
    <dbReference type="GeneID" id="30027341"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070056">
        <property type="term" value="C:prospore membrane leading edge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904853">
        <property type="term" value="P:protein localization to ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="356" mass="41002" checksum="4B4E6305B8F1868F" modified="2016-10-05" version="1">MTDFQAYKGTNFYVPSRYKIQEVLGRGSYGVVCVAIDTKSQDPVKLAVKKICKILQREILLKRAIRELKLMRFFRGHRNIVSLVDVYFITLSPYEGLYCFQEHMDYDLARVLYSTVQFLEFHIRNFLYQVVCGIKYIHLANVVHRDLKPGNILVSSRGVLKICDFGLARAISGSPSNRSEEPITNYVATRWYRAPELILRSTHYGKEVDMWAVGCILGELYGRQPLMPGKSSVHQLHEIIKYLGSPPPHISIRRDWDLPGGERKAVNWSTVYPFASADSLDLLGQLLKWEPTARLDVEQTIAHSFFKKIRDITCEPQSRSVFNFGKEENEDDLNKLHGLLKEEVAAFQTERNKRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F0</accession>
    <name>A0A1A0H5F0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FAD/NAD(P)-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33501</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709846.1">
        <property type="nucleotide sequence ID" value="XM_018856311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F0"/>
    <dbReference type="GeneID" id="30029287"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="23"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="423" mass="47496" checksum="533993FF80ACBBFB" modified="2016-10-05" version="1">MSTSIIRKTQDAIQKNTPSFHTNVLIVGGSYAGLSALIAIKNHLRERNRLQKISVTIVEPKAGFLNVLGVPRAIVDIEFAKTQFVPFESLEDIVLDHVISNDEFVIDTLGKTVKPNNNDTVEVTYVQGSITDLRSKSASYHLNNRTDEVGSIEFNYSVIATGRNRHWPTSPLAYNYQSYMEEMVEFRRQVDTCQKIAVIGAGAVGIEIAGDVKNNCPDKEVFLIHPHDKFPPEPLPEKFKDLIRDSLERGGVKVMTGYRVKSELDDHELEFTNGEKFKTDLNYWCSKFENNTKLLSSELETFISPKNNIHVNDFLQLTHPDSQEHLPHVFAIGDLVELPMIKSAGWALYMGRQVANNIVSLIFDGVLVEPMPDLKLMPKGMVIVAGDNEIVSALDEVLELNNELFVEEYKDYCIGKIRATLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F5</accession>
    <name>A0A1A0H5F5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L21-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_79710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709685.1">
        <property type="nucleotide sequence ID" value="XM_018859115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F5"/>
    <dbReference type="GeneID" id="30032091"/>
    <dbReference type="OrthoDB" id="1539250at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3260:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="160" mass="17931" checksum="80C0ADCE04327DFC" modified="2016-10-05" version="1">MGKSRGYRSGTRYMFQRDFKKHGIVNLSTYLTIYKVGDIVDIKANGSIQKGMPHKYYHGKTGIVFNVTKSSVGVIINKVVGNRYIEKKVNLRVEHVKHSACRKEFLDRVKINAGLREEAKATGKQVVLKRQPAQPRPAKIVSADGNAAQTLAPVPYETFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F9</accession>
    <name>A0A1A0H5F9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glucan 1,3-beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_33349</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709674.1">
        <property type="nucleotide sequence ID" value="XM_018856272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F9"/>
    <dbReference type="GeneID" id="30029248"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046557">
        <property type="term" value="F:glucan endo-1,6-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF43">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="425" mass="48313" checksum="D27791F617F3A18B" modified="2016-10-05" version="1">MEAHWTSYFLDEDWKWLTENKVNSLRLPIGFWNIDGGKYTTGTSFAEFSEMYQNSWLIIKSHYIEPAAKRGISILVDIHGVPGGANVNDHSGEVYGSPEFWITPSFQNLLIEAAVFVFEDLKEYDNISGVLLINEASYDFNSEQQNFYAKAINAIREKEKTIPVVISDGWNPDSWIQWVSSNQNPNAPAGIVVDHHVYRTFTPENKLRTAQKIIADLYDDLLPSVNNNGNDVDFMVGEWSCVMDSETWSKSGVSPSDDNDPGRKKLVAEFGSKELELIMKRASTGSYFWTYKFESGNGGEWDFRQQLGHGFKAPQVSVPDNSTFKTTLDREYAAHADYWDSRLPKETNDHERYKEGFIAAWKDGVTYAKVGALVGRKQAVKSVRLQEHLQSKGDLKYLWEWKQGYDKGLEEFNKNALQAAEGTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5H3</accession>
    <name>A0A1A0H5H3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_26574</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709871.1">
        <property type="nucleotide sequence ID" value="XM_018855320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5H3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5H3"/>
    <dbReference type="GeneID" id="30028296"/>
    <dbReference type="OrthoDB" id="2405700at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038910">
        <property type="entry name" value="Hua1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28031:SF1">
        <property type="entry name" value="PROLINE-RICH PROTEIN HUA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="223" mass="24729" checksum="ACC92D3332BF98A2" modified="2016-10-05" version="1" fragment="single">PPSYEEALNSPPVPPGRPQADPQENPYSSAPPPQPPRPQAPVFPNRPQQPQGPGHTQNPQSALLYTNNPNLPFDYPRGYYCNKCKNTGFREKNMKPCSHCWSKFFKDKAYNPNPSLNFKYPRGYICDKCRNTGTKVKNGRSCQDCYSRFAPRNLSSQAPITYSLPAFGFAPGGFAPPNVPQYVQPGDPRMGGTMCGRCRGSGRTTFFLDKEICLVCGGVGRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5L2</accession>
    <name>A0A1A0H5L2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL24-related N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_220105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709735.1">
        <property type="nucleotide sequence ID" value="XM_018855131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5L2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5L2"/>
    <dbReference type="GeneID" id="30028107"/>
    <dbReference type="OrthoDB" id="1727108at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000002">
        <property type="entry name" value="60S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL24-related N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="60"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="89"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="131" mass="15033" checksum="DCDDFCDA0381BC85" modified="2016-10-05" version="1">MKIEIDSFSGAKIYPGRGTLFVRGDSKIFRFQSSKSASLFQQRKNPRKISWTVLYRRQHKKGITEETKKRRTKKTVKHQRPIEGASLDFIKEKRNQKPAERKAAGIQAGTNEKVSKMGQKGAFQKVHATSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Y0</accession>
    <name>A0A1A0H5Y0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HCP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709855.1">
        <property type="nucleotide sequence ID" value="XM_018854399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Y0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Y0"/>
    <dbReference type="GeneID" id="30027375"/>
    <dbReference type="OrthoDB" id="1658288at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="41133" checksum="FEBC98534AF9DFE4" modified="2016-10-05" version="1">MLRRVGIRFQLTTSRPSLDSILPKKKTINRLLFDNDSRLAYKKAMPILNTVYANLDSPQDIRLPSHAKHDDLMMLRSILHDLRSVTNSINKNLVDLENELVEQAAELGNNDAIAMLAFEAVASKDTSKEDHAHANKLIRQLTEQKHPLVFKLAGDFAFSKSYHEQAAQYWQQFLELEDDTILASHVHSNLGIYYYHYMNPRPNLGEAKLCFEKAIRFGQLDSHIVKAHYYLGQLYSITDPVLSRYHLEISATKGLQESLPSLGFMELNVFNNVSKALEWFKLGVEANSDISCLIGQFDAHSRAGSNGKAIKILANLRGLKEKLDKILAKGINHVPASYRELAASNQVLLNTFFATRSDAIKNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6E6</accession>
    <name>A0A1A0H6E6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_33245</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710129.1">
        <property type="nucleotide sequence ID" value="XM_018856253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6E6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6E6"/>
    <dbReference type="GeneID" id="30029229"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="17317" checksum="DF382FF683E83DC6" modified="2016-10-05" version="1">MEHSANGGMGHDMPMHDMCKMNMLFTWEWENTCVVYKWWHVKTAPGFVGTVLALVLLSMFYEFTRSWISLWKARWTPNSIAPNTPLVSGRASKSIRIKSAALYAFQVGFSFMLMLVFMTYNGWYMLAIVAGAGFGFYLWGDEKEAKSMLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6K6</accession>
    <name>A0A1A0H6K6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710244.1">
        <property type="nucleotide sequence ID" value="XM_018857855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6K6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6K6"/>
    <dbReference type="GeneID" id="30030831"/>
    <dbReference type="OrthoDB" id="3991365at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020266">
        <property type="entry name" value="Tom6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17112">
        <property type="entry name" value="Tom6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="53" mass="5768" checksum="0FA0AC8E06641F47" modified="2016-10-05" version="1" fragment="single">SSTYPKMAPKQEPSRFEQIKNAPAFTAAVQVALFAAGVAFIQSPLMEFLVPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6K8</accession>
    <name>A0A1A0H6K8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_45779</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710070.1">
        <property type="nucleotide sequence ID" value="XM_018857958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6K8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6K8"/>
    <dbReference type="GeneID" id="30030934"/>
    <dbReference type="OrthoDB" id="259769at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.10:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028363">
        <property type="entry name" value="RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500154">
        <property type="entry name" value="RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="155" mass="17351" checksum="DDC2B0A319C67525" modified="2016-10-05" version="1">MSDVEEPYNEAPENFEDFDAPDHFSDDDFAEPEPAAHGEPDVAMKAEDGRTIVNGGYSEADAQVRVRAAKALAIARDERSTTPYMTKYERARVLGTRALQISLNAPVLVDIEGETDPLQIAMKELAQRKIPLVLRRYLPDGSYEDWGCDELIIEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6N2</accession>
    <name>A0A1A0H6N2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sec20-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_46183</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SEC20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710148.1">
        <property type="nucleotide sequence ID" value="XM_018858001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6N2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6N2"/>
    <dbReference type="GeneID" id="30030977"/>
    <dbReference type="OrthoDB" id="46868at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005606">
        <property type="entry name" value="Sec20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056173">
        <property type="entry name" value="Sec20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825">
        <property type="entry name" value="BNIP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12825:SF0">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SEC20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03908">
        <property type="entry name" value="Sec20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Sec20 C-terminal" evidence="11">
        <location>
            <begin position="176"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="33320" checksum="9D4DAC8199B2F510" modified="2016-10-05" version="1" fragment="single">MGFPPANLELESIKCLFGEIDDLQSQVLLQISRISKRSSSDDTEPVQIFREKTVKTATTLIHKIADLLRVTDLKILRIPAIAASEHYLHETLDLYNGKVKHLRRKLKESQLAAYRMENDVVHQQRLREYCPAKNLEGSDPTEPVDAKQALFEGRSAKHEGQRGNDKTKSVDEQILTQNKNISSSLQHSKQLMTMLVMQTELNIDTLDQQSKDLMQLNDKLIDMETVLTKSREIIKFIERQDKHDKRRIYAAILFLLLCSAWVLWRRVLKVPVKMLVWTLLKMFGVVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U1</accession>
    <name>A0A1A0H6U1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710198.1">
        <property type="nucleotide sequence ID" value="XM_018859108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U1"/>
    <dbReference type="GeneID" id="30032084"/>
    <dbReference type="OrthoDB" id="1106148at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055304">
        <property type="entry name" value="CHCHD2/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523:SF2">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13523">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 2/NUR77"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15043" checksum="C9D12FC6DE406E1D" modified="2016-10-05" version="1">MARQRRSAPAPRTQTRSAHTAAAPAPVAAAPHPPAPVAQPQVASQGPGLFGQMASTAAGVAVGSTIGHTLGAGITSMFGGSSTPAEQPQQQVAATQQQSTQEQARTCDSDARSFTRCLEENSGNMQICDYYLQQLKACQEAARSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Y6</accession>
    <name>A0A1A0H6Y6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_208871</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710384.1">
        <property type="nucleotide sequence ID" value="XM_018855020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Y6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Y6"/>
    <dbReference type="GeneID" id="30027996"/>
    <dbReference type="OrthoDB" id="341482at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019321">
        <property type="entry name" value="Nucleoporin_Nup88"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037700">
        <property type="entry name" value="NUP88/NUP82"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257:SF0">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13257">
        <property type="entry name" value="NUCLEOPORIN NUP84-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10168">
        <property type="entry name" value="Nup88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="787" mass="87145" checksum="F755A39CB1EF8369" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H709</accession>
    <name>A0A1A0H709_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein L34e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_46025</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710220.1">
        <property type="nucleotide sequence ID" value="XM_018857982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H709"/>
    <dbReference type="STRING" id="869754.A0A1A0H709"/>
    <dbReference type="GeneID" id="30030958"/>
    <dbReference type="OrthoDB" id="277449at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="118" mass="13379" checksum="85EB9960FBA2C28A" modified="2016-10-05" version="1">MAQRVTYRRQTPYNTRSNKIKVVKTPGGKLIAQHVKKQGSKVKCGDCGIALPGVASLRPRQYAQISKTHKTVQRAYGGSRCANCVKERIVRAFLIEEQKIVKRVLKEQQEKERKSAKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D1</accession>
    <name>A0A1A0H7D1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_32872</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710333.1">
        <property type="nucleotide sequence ID" value="XM_018856151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7D1"/>
    <dbReference type="GeneID" id="30029127"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="568"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="570"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="614" mass="71407" checksum="D7C42978507EDECC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D2</accession>
    <name>A0A1A0H7D2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein G</fullName>
            <shortName evidence="9">snRNP-G</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_59118</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710529.1">
        <property type="nucleotide sequence ID" value="XM_018858306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7D2"/>
    <dbReference type="GeneID" id="30031282"/>
    <dbReference type="OrthoDB" id="2146at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01719">
        <property type="entry name" value="Sm_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000023">
        <property type="entry name" value="Small nuclear ribonucleoprotein G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044641">
        <property type="entry name" value="Lsm7/SmG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034098">
        <property type="entry name" value="Sm_G"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10553:SF2">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="2"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="74" mass="8006" checksum="0E36EBE62BF93567" modified="2016-10-05" version="1">MVSAPELKSYLDKKVSVQLNGSRTVTGTLRGYDVFMNITMADALEHDPKGEQLALGTIVVRGNSIVSVEALEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7J4</accession>
    <name>A0A1A0H7J4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79273</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710475.1">
        <property type="nucleotide sequence ID" value="XM_018859028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7J4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7J4"/>
    <dbReference type="GeneID" id="30032004"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013870">
        <property type="entry name" value="Ribosomal_mL54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L54, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08561">
        <property type="entry name" value="Ribosomal_L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="11844" checksum="FB2CA9BAA75C339D" modified="2016-10-05" version="1">MFKLPARILHFGLVRPFSTSFARLNAPKSSCAAGTVLNLKVRKNGDEPVALEDLEYPEWLWDILDKSKTEAALKEEDPIKWRRKQNSKRTTAKIKNNNFLAQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K5</accession>
    <name>A0A1A0H7K5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_44637</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710408.1">
        <property type="nucleotide sequence ID" value="XM_018857820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K5"/>
    <dbReference type="GeneID" id="30030796"/>
    <dbReference type="OrthoDB" id="1555531at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000014">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000053">
        <property type="entry name" value="Ribose-5-phosphate isomerase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="235" mass="25182" checksum="12EFD5C01175DC2F" modified="2016-10-05" version="1">MSSTIESAKMRAAYRAVDENLPDNAKVVGIGSGSTVVYVAERLSQLANKDSFVCIPTGFQLKQLILDHGLHVGTIEQYPSLDIAFDGADECDDKLNLIKGGGACLLQEKLVAEAAATFIIVADYRKKSRALGTEWTQGVPIEIVPNAYAKVTLDLKRLGARNVDLRQGGKAKAGPVITDNNNFLIDADFGPIADPRGLHAKIKSLCGVVDSGLFTNLARKAYFGEQDGAVSMWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7P3</accession>
    <name>A0A1A0H7P3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SYO1-like TPR repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_209872</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nuclear import and ribosome assembly adapter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710437.1">
        <property type="nucleotide sequence ID" value="XM_018855027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7P3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7P3"/>
    <dbReference type="GeneID" id="30028003"/>
    <dbReference type="OrthoDB" id="288703at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13394">
        <property type="entry name" value="Syo1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052616">
        <property type="entry name" value="SYO1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057990">
        <property type="entry name" value="TPR_SYO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13347:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25567">
        <property type="entry name" value="TPR_SYO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SYO1-like TPR repeats" evidence="3">
        <location>
            <begin position="412"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00049983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="639" mass="72276" checksum="2D340A9635F388D4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U1</accession>
    <name>A0A1A0H7U1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Endoplasmic reticulum chaperone BiP</fullName>
            <ecNumber evidence="3">3.6.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Immunoglobulin heavy chain-binding protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_73161</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER. Is required for secretory polypeptide translocation. May physically associate with SEC63 protein in the endoplasmic reticulum and this interaction may be regulated by ATP hydrolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710616.1">
        <property type="nucleotide sequence ID" value="XM_018858410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7U1"/>
    <dbReference type="GeneID" id="30031386"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034099">
        <property type="term" value="C:luminal surveillance complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070972">
        <property type="term" value="P:protein localization to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10241">
        <property type="entry name" value="ASKHA_NBD_HSP70_BiP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042050">
        <property type="entry name" value="BIP_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="658"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="678" mass="73632" checksum="CF6D07E1CE53DCB9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H803</accession>
    <name>A0A1A0H803_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_13129</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 10"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710640.1">
        <property type="nucleotide sequence ID" value="XM_018854309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H803"/>
    <dbReference type="STRING" id="869754.A0A1A0H803"/>
    <dbReference type="GeneID" id="30027285"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="758" mass="86323" checksum="8E5E851C95B56518" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H837</accession>
    <name>A0A1A0H837_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33089</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710675.1">
        <property type="nucleotide sequence ID" value="XM_018856216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H837"/>
    <dbReference type="GeneID" id="30029192"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="73" mass="7870" checksum="A281634DDD5D81A5" modified="2016-10-05" version="1">MQKHAQLNPCLFPACPSSHLGSISLALAARVRRPHSHPASAAGRERARRQARERGSDMCTTPVHHGIDGLASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H899</accession>
    <name>A0A1A0H899_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_192799</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710727.1">
        <property type="nucleotide sequence ID" value="XM_018854855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H899"/>
    <dbReference type="GeneID" id="30027831"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="8908" checksum="1E9194DD9D3076B4" modified="2016-10-05" version="1">MSVLQCQSCMRTLVSLLGWIGGRTLWGCIRQRCTACNKNDLKTTAFSSSPLVFSILCCSFIYMQRSIQGDVVIWCFSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8D3</accession>
    <name>A0A1A0H8D3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_32261</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710762.1">
        <property type="nucleotide sequence ID" value="XM_018856019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8D3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8D3"/>
    <dbReference type="GeneID" id="30028995"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291690" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="336" mass="36390" checksum="1F4159381E916E2C" modified="2016-10-05" version="1">MILGVIRTAITLNFVALVTSMEIVDRTVANNKAEFAEESLTVSAEVYLSIVNSSRVTFLDSVRNYGSLYVTNRNNQDVWVRMSGQDFENSGTVSFSCLTTPVLSDYHIMATRSFVNTGNMYFGVYGGDYGASPFSVTSVATWTNSGMILFLVAHGESAQLQIQRYTPDNGYRSITNTGSLCLNNTHWPVQTNIEGNGCIIVGSGGQLDLQFSESTHGIAEDQTIYLASSDSLLKILGLESYSSEPPVIKLAGLGGSNRIQFQTYSTQTYRYYTSTGLLNVFVDNVRKVSFNIGIGYELGLFDSTSGILSYSGEPPDSAPDVCKCGTSFPAVPNTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8H9</accession>
    <name>A0A1A0H8H9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_195825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710817.1">
        <property type="nucleotide sequence ID" value="XM_018854872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8H9"/>
    <dbReference type="GeneID" id="30027848"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="263" mass="29415" checksum="F362C476FC7DFCD8" modified="2016-10-05" version="1">MDTTVNVAMLPIANPDESGWQVQGRKRRNSRTRTGKKTDMWMLIGMTSSEKWFALRSENAVPESVLFNNPYLVLADIDENDYQEPRPSRTYTKVPEKLELSSLGNVISGCSLIMVLTMDSYEVLNLNVVTNIMDKARESDDEKDLGIFHESTQNLTKDLPTDLGMGSTLASKQVGTIAKMPDIPETDIDDHGWKVQGRKHRNCRKRPQPTVSGAESLATPPTDPKCTLGPKPEMKSLYSQNPYLVLAEIGDDELHGSPPRRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8L2</accession>
    <name>A0A1A0H8L2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Septin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_42966</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710878.1">
        <property type="nucleotide sequence ID" value="XM_018857604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8L2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8L2"/>
    <dbReference type="GeneID" id="30030580"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061163">
        <property type="term" value="P:endoplasmic reticulum polarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010458">
        <property type="term" value="P:exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="25"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="528"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="538"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="555"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="583" mass="64842" checksum="F4D2D3039F893404" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8L5</accession>
    <name>A0A1A0H8L5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710857.1">
        <property type="nucleotide sequence ID" value="XM_018856034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8L5"/>
    <dbReference type="GeneID" id="30029010"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508812" evidence="1">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6651" checksum="01ECD3CD2FC6C1AC" modified="2016-10-05" version="1">MQQRRRARAVHTPVVFLLLAASVLLAGKDRHAVPLSGRGAGFWDRFAFGYRLCVPACRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Q0</accession>
    <name>A0A1A0H8Q0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_44302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AVL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710785.1">
        <property type="nucleotide sequence ID" value="XM_018857772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Q0"/>
    <dbReference type="GeneID" id="30030748"/>
    <dbReference type="OrthoDB" id="26278at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018307">
        <property type="entry name" value="ABL9/DENND6_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051731">
        <property type="entry name" value="DENND11/AVL9_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017:SF1">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31017">
        <property type="entry name" value="LATE SECRETORY PATHWAY PROTEIN AVL9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09794">
        <property type="entry name" value="Avl9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="149"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="647"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="649"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="696" mass="77819" checksum="19862409C517A7EE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U3</accession>
    <name>A0A1A0H8U3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78725</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710824.1">
        <property type="nucleotide sequence ID" value="XM_018858928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8U3"/>
    <dbReference type="GeneID" id="30031904"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070145">
        <property type="term" value="P:mitochondrial asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF34">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="8">
        <location>
            <begin position="149"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="485" mass="52805" checksum="C9FA6081F5C03B9B" modified="2016-10-05" version="1">MLPQRLVARLSLLAPTIRQLMETPPEPHTKATARGHIKALRTFKNVGFMDLSDGSGHGTLSVIVSDPGAVFAAHQYKVGQSVEVTGRWAPSRGRQPFELACDTQDPAHGIAVLGDVAPDYPIQKKRHTLAFLRSAPTLRHRTALLSSVLRMRARAEAAVAAFFLLHDVVKVAPPLITASDCEGAGEQFQVRPLGESTADGGGGGGFFGTAAYLTVSTQLHLEVLALSLNRVWTLTPCFRAETSNTPRHLSEFWMLEAELCYVTSVGQLTAFVEQMVRHVVAHLAAGGEWAAGAQHDLLAARHSRDDVARIRAAWAQVLLPAPWPSVTYSEALETLNALKYKGRLQGRLAWGAPLLTEDEKWLAGCHYQLPVFVTDYPKAQKPFYMRASGDAARPTVACFDLLVPELGELVGGSLREHDLATLEAEMASRGMLRDAMRWYLSTRENGSVPHGGFGMGFERLLAYLAAVDNIRDISAFPRAPNVCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8V3</accession>
    <name>A0A1A0H8V3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_32328</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710834.1">
        <property type="nucleotide sequence ID" value="XM_018856029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8V3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8V3"/>
    <dbReference type="GeneID" id="30029005"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06614">
        <property type="entry name" value="STKc_PAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000011">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000385">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF22">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE SAMKA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="15">
        <location>
            <begin position="497"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="855"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="339"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="404"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="576"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="576"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="587"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="614"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="666"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="884"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1133" mass="121600" checksum="E21596D89CAA5E2A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H952</accession>
    <name>A0A1A0H952_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_202541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711176.1">
        <property type="nucleotide sequence ID" value="XM_018854947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H952"/>
    <dbReference type="STRING" id="869754.A0A1A0H952"/>
    <dbReference type="GeneID" id="30027923"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="167" mass="18533" checksum="7BA8D04F30FF943E" modified="2016-10-05" version="1">MCFGNPGSVFWEFRECVLGIPGMCSGNPGNVFWESWECVLGSLGNVFWESWECVLGSLGVCSGNPGNVFWESWECVLGILGVCSGKPGSVFWECLLRMSSRNVFWESWECVLGIPGMCSGNPGNVFWVLWAHASGVAYSTVADFIHNVRNELTPKQIWSTVQIYVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H990</accession>
    <name>A0A1A0H990_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED16</name>
        <name evidence="13" type="ORF">METBIDRAFT_230170</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711108.1">
        <property type="nucleotide sequence ID" value="XM_018855240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H990"/>
    <dbReference type="STRING" id="869754.A0A1A0H990"/>
    <dbReference type="GeneID" id="30028216"/>
    <dbReference type="OrthoDB" id="4139168at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048338">
        <property type="entry name" value="Mediator_Med16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048339">
        <property type="entry name" value="Mediator_Med16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021665">
        <property type="entry name" value="Mediator_Med16_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13224:SF6">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13224">
        <property type="entry name" value="THYROID HORMONE RECEPTOR-ASSOCIATED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20719">
        <property type="entry name" value="Med16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11635">
        <property type="entry name" value="Med16_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med16 N-terminal" evidence="11">
        <location>
            <begin position="181"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Mediator complex subunit 16 C-terminal" evidence="12">
        <location>
            <begin position="865"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="131"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="990" mass="111355" checksum="57DA3DB8480B61E9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9M0</accession>
    <name>A0A1A0H9M0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">U3 small nucleolar ribonucleoprotein protein IMP4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_43283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711346.1">
        <property type="nucleotide sequence ID" value="XM_018857638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9M0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9M0"/>
    <dbReference type="GeneID" id="30030614"/>
    <dbReference type="OrthoDB" id="10253204at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140691">
        <property type="term" value="F:RNA folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000001">
        <property type="entry name" value="IMP4, U3 small nucleolar ribonucleoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF2">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="79"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="285" mass="32899" checksum="3F4F3624E9DA03B3" modified="2016-10-05" version="1">MIRRQARERREYLYKKALELQETAQIEKRQKLKAALASGKALSKELSEDTELHKDFRYDESEQVDIDDEYLALSGISDPRIIVTTSRNPSVRLLQFSKEIKLMFPNSLKLNRGNYVIPELVKSCAKAAINDLIVLHEHRGVPTSLTVSHFPHGPTAIFTLHNVKLRHDLPNMGNISEVYPHLIFENFSTALGQRVVKILKHLFPPGAKKDSSRVITFINNEDFISVRHHVYIQTKDGVELSEVGPRFEMRLFELRLGLLDNKDADVEWKVKRFIRTANRKNYLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9M7</accession>
    <name>A0A1A0H9M7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78923</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711105.1">
        <property type="nucleotide sequence ID" value="XM_018858967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9M7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9M7"/>
    <dbReference type="GeneID" id="30031943"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF30">
        <property type="entry name" value="MATERNAL EMBRYONIC LEUCINE ZIPPER KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="32915" checksum="69606D25B8F1905E" modified="2016-10-05" version="1">MIWIKGLREYKGSKTLLGEGISGKVELYEKGTKRYVIKTYHGKELHETRKEYRARILYEYQLLNSLQHPNIIRAFKSVVSANGLQVKVFIEAGSPDLSVLLQRHGSSLCITTLTALWKQVCEGVCYLHERELCHRDLKLENLVMNPEFTQIKIIDFMTATKCEGPVVGIVGSSRYMAPEMASRISYEGKSVDMWSLGIILYRLLSRKFPWRQAMHLDPDFKIYAETRGSGSQLSDVYGKKIGGKTHLPGWSPSFPVLMSFLLEINPSNRSTIQQVFEDEWFASIQSHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q3</accession>
    <name>A0A1A0H9Q3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Creatinase/aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32587</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711130.1">
        <property type="nucleotide sequence ID" value="XM_018856091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Q3"/>
    <dbReference type="GeneID" id="30029067"/>
    <dbReference type="OrthoDB" id="3209743at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031615">
        <property type="entry name" value="Zfn-C6H2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43330:SF7">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15801">
        <property type="entry name" value="zf-C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52013">
        <property type="entry name" value="ZF_C6H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C6H2-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="216" mass="24212" checksum="10D1A2D461ADA21E" modified="2016-10-05" version="1" fragment="single">MATCSSPQCGKDSESALKCPVCLKNGISSVFCNQTCFRGGWAMHKAVHPAEDNKKFDPFPNFKYTGDLRASYPLSSRRAVPQHIKKPDYAQDGRPLSEIKNDRLGKITILTESEIKKMRKVGLISRQILDKVASHIAPGITTDELDAILHKECLHHNAYPSPLNYYNFPKSMCTSLNEVICHGVPDKTVLQDGDILNLDVTIYLHGFHSDLNETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q8</accession>
    <name>A0A1A0H9Q8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphatidylinositol 3-kinase VPS34</fullName>
            <ecNumber evidence="4">2.7.1.137</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Vacuolar protein sorting-associated protein 34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_24769</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Multifunctional phosphatidylinositol 3-kinase involved in acidification of vacuoles, pH-dependent cell growth, and autophagocytosis. Plays an important role in protein transport and virulence. Component of the autophagy-specific VPS34 PI3-kinase complex I essential to recruit the ATG8-phosphatidylinositol conjugate and the ATG12-ATG5 conjugate to the pre-autophagosomal structure. Also involved in endosome-to-Golgi retrograde transport as part of the VPS34 PI3-kinase complex II. This second complex is required for the endosome-to-Golgi retrieval of PEP1 and KEX2, and the recruitment of VPS5 and VPS7, two components of the retromer complex, to endosomal membranes (probably through the synthesis of a specific pool of phosphatidylinositol 3-phosphate recruiting the retromer to the endosomes). Finally, it might also be involved in ethanol tolerance and cell wall integrity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the autophagy-specific VPS34 PI3-kinase complex I composed of at least VPS15, VPS30, VPS34, and of the VPS34 PI3-kinase complex II composed of VPS15, VPS30, VPS34 and VPS38. Interacts with VMNA7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711135.1">
        <property type="nucleotide sequence ID" value="XM_018855283.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q8"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Q8"/>
    <dbReference type="GeneID" id="30028259"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032120">
        <property type="term" value="P:ascospore-type prospore membrane formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051365">
        <property type="term" value="P:cellular response to potassium ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00896">
        <property type="entry name" value="PI3Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000002">
        <property type="entry name" value="Phosphatidylinositol 3-kinase catalytic subunit type 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057756">
        <property type="entry name" value="PI3-kinase_type3/VPS34_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008290">
        <property type="entry name" value="PI3K_Vps34"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF7">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE CATALYTIC SUBUNIT TYPE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000587">
        <property type="entry name" value="PI3K_Vps34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2 PI3K-type" evidence="16">
        <location>
            <begin position="37"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="15">
        <location>
            <begin position="309"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="14">
        <location>
            <begin position="647"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="997"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA20613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="997" mass="111007" checksum="1897213BF0BB6704" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9T8</accession>
    <name>A0A1A0H9T8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711169.1">
        <property type="nucleotide sequence ID" value="XM_018856101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9T8"/>
    <dbReference type="GeneID" id="30029077"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="78" mass="8868" checksum="E16922FA1EDC07FA" modified="2016-10-05" version="1">MYLVSKVAPPPSVSHGLDSPLGPYLFMYWTISCQERAFRAQTRAFDQACLFMKAVVKYVFLQTAWSNTSGNKRHGHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA70</accession>
    <name>A0A1A0HA70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PHP domain-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_78038</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711421.1">
        <property type="nucleotide sequence ID" value="XM_018858806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA70"/>
    <dbReference type="STRING" id="869754.A0A1A0HA70"/>
    <dbReference type="GeneID" id="30031782"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="290"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="318" mass="34590" checksum="DB4C9D3A0343B525" modified="2016-10-05" version="1">MYDINVPWPSSSYALEPSPAELTNFYNTIAMLHSFGYNYLAINFIVDEAVKLPVNSPSKLNPVPMAELRKRFGAFSKLRLFSRITLVVSDPAKAQSILKINNTGAFDIVAVQPTTEKALQLTVTNLDIDLLSLPMASRLPFFLKHKTVGLALAKGVKFEICYSGLIAGPAGYESSTALGASGHISRKNFFSNCLQLIRASRCRGLVFSSGATEPLHVRNYADVLAIMNSLGLKVSNSKEGFLAHPESVLVKGRLRIKSHKQTVIVGDEAGTDSRAGALFGGEDSKNGVLDDYKKKKGAGKRSLEHQADRAKRVKTMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAI2</accession>
    <name>A0A1A0HAI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_176130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711649.1">
        <property type="nucleotide sequence ID" value="XM_018854725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAI2"/>
    <dbReference type="GeneID" id="30027701"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="119" mass="13915" checksum="06691C1E403E24E5" modified="2016-10-05" version="1">MFPNIWQIKLRLRYPNSRVINGNMDRICPIDPIYWITQGCRQIFENMRPGESEMFLLRFGIDLIPRILDLLWLILQLFIFSKAVTIISGLQSSISLRYKLMSTLAWYSLFVVGLLSSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ4</accession>
    <name>A0A1A0HAQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711585.1">
        <property type="nucleotide sequence ID" value="XM_018857429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAQ4"/>
    <dbReference type="GeneID" id="30030405"/>
    <dbReference type="OrthoDB" id="1888931at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050085">
        <property type="term" value="F:mannitol 2-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050664">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000090">
        <property type="entry name" value="NADP-dependent mannitol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008">
        <property type="entry name" value="BENZIL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43008:SF13">
        <property type="entry name" value="L-XYLULOSE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="285" mass="30288" checksum="B804B9024B2735FE" modified="2016-10-05" version="1">MTASDNQYTTIIKPQQDEAPVPFPAPTLTGTAFERFSMAGKVTVITGAAGSIGGALADAFASAGSHVAIMDFRYNPTIDKTLSEKYGVKVQSYTVDVTNAGQVQKTVQDIEADFGTIDTFIANAGVVWTTGSILNEASTYEEWARVFNVNVHGVFNCAKSVGEVFKRKGKGSLIMTASISGHIVNVPNYQTGYNASKAAVVHMGKSLAVEFAGFARVNTVSPGYTDSGLSDFVPTEQRAKWWGLTPLGREAQPEELVGAYMYLASDLASFTTGTDIQVDGGYCAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ7</accession>
    <name>A0A1A0HAQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711478.1">
        <property type="nucleotide sequence ID" value="XM_018857522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAQ7"/>
    <dbReference type="GeneID" id="30030498"/>
    <dbReference type="OrthoDB" id="3997547at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="170" mass="19241" checksum="4CBFFC57D95AB186" modified="2016-10-05" version="1">MSFVPKRKRDTEPDQKKPKAAFGDKEREMLSQNNEWPMYNHPDGGTVKITPWGALRQWIDPATGYAKTKFEDASFQEVSFEMSPSNTNNQCYELPTTPMSLHTSASGCSSPAYGDKLNFSPTNEAELYVVEGYGQPRESGQRPTQQYAQEQEHYLGMEESEDFSDDTAMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAU4</accession>
    <name>A0A1A0HAU4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zinc finger C2H2 LYAR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_14544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the UPF0743 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711512.1">
        <property type="nucleotide sequence ID" value="XM_018854516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAU4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAU4"/>
    <dbReference type="GeneID" id="30027492"/>
    <dbReference type="OrthoDB" id="21474at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.490:FF:000001">
        <property type="entry name" value="cell growth-regulating nucleolar protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039999">
        <property type="entry name" value="LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014898">
        <property type="entry name" value="Znf_C2H2_LYAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100:SF10">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08790">
        <property type="entry name" value="zf-LYAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51804">
        <property type="entry name" value="ZF_C2HC_LYAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger C2H2 LYAR-type" evidence="10">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="65"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="116" mass="12971" checksum="D9AC17856CCA9A6F" modified="2016-10-05" version="1" fragment="single">MVSFSCEVCNDTVIKKKLDQHRQRCYDAYFTCIDCSTTFHGNDHKSHTQCISEAEKYEKALYKGKPSKAAGKTNSAQAKSGKPAAKKEDEKNVAEETDSKKLKKAKDTTAEESKDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAV7</accession>
    <name>A0A1A0HAV7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711656.1">
        <property type="nucleotide sequence ID" value="XM_018855906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAV7"/>
    <dbReference type="GeneID" id="30028882"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6778" checksum="73E8EC734CE9C45B" modified="2016-10-05" version="1">MCALWCASCANKVFVVEMILEEVSSAMIIVEASIAKMCIVKMFILQMFIVRASIAKRFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAY4</accession>
    <name>A0A1A0HAY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_42300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711681.1">
        <property type="nucleotide sequence ID" value="XM_018857525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAY4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAY4"/>
    <dbReference type="GeneID" id="30030501"/>
    <dbReference type="OrthoDB" id="10250282at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001110">
        <property type="entry name" value="UPF0012_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088:SF27">
        <property type="entry name" value="DEAMINATED GLUTATHIONE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23088">
        <property type="entry name" value="NITRILASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01227">
        <property type="entry name" value="UPF0012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="324" mass="36107" checksum="70D17DDE9C96BC64" modified="2016-10-05" version="1">MSTKLRIAVGQMCSSSNVRMNSRVVCKLIDRAVERKAKVLFLPEASDYISRDAAHSVSLAKQTKADFINIIQKKLQSVLSKQSLGALCVAVGVHEPTKGVNKDTKVQNNQLWISELGVIEQRYQKIHLFDINIPNGPIFQESRSVEAGNKILCPFPISDNAPGFKVGFSICYDIRFPELAARLRQMGANILTYPSAFTTKTGEAHWLELGRARAIDSQCYVVMAAQCGEHDIYADILEGKEKLDNNPQGNRTKRVSYGQSVIFGPWGDILAKGPTYLEAECSDAVDAEGDYYEIFDAEINLENLEKIRTNLPVFSHRRPDVYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB09</accession>
    <name>A0A1A0HB09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_42780</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711685.1">
        <property type="nucleotide sequence ID" value="XM_018857586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB09"/>
    <dbReference type="STRING" id="869754.A0A1A0HB09"/>
    <dbReference type="GeneID" id="30030562"/>
    <dbReference type="OrthoDB" id="5579281at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902657">
        <property type="term" value="P:protein localization to prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040345">
        <property type="entry name" value="Mug56/Spo71"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039486">
        <property type="entry name" value="Mug56/Spo71_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057379">
        <property type="entry name" value="PH_SPO71"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029217">
        <property type="entry name" value="Spo7_2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28076:SF1">
        <property type="entry name" value="PROSPORE MEMBRANE ADAPTER PROTEIN SPO71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28076">
        <property type="entry name" value="SPORULATION-SPECIFIC PROTEIN 71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15404">
        <property type="entry name" value="PH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23207">
        <property type="entry name" value="PH_SPO71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15407">
        <property type="entry name" value="Spo7_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01316">
        <property type="entry name" value="Spo7_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="852"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1032" mass="118081" checksum="DC22ADD8D6A4D1D4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB21</accession>
    <name>A0A1A0HB21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711834.1">
        <property type="nucleotide sequence ID" value="XM_018855934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB21"/>
    <dbReference type="STRING" id="869754.A0A1A0HB21"/>
    <dbReference type="GeneID" id="30028910"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="429"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="447" mass="49588" checksum="0C8981AE9D409AA1" modified="2016-10-05" version="1" fragment="single">MSFTATATREVAPTINLSDFSHPPTTQIHAEDAGAETRLMPLAPPAKPGAAQAQFSDDDDDEFNLDMVTMDMNFEQAYSMYTQLQQKNALLEVEHLQKAQQLNHKQAHEQIRLFLDEVLLSQQAPRPAGADKQWARAAELYPGDYSSQCHARFYPETLLDQTGGPSRNMAHTVEAVQHAACHAALPDISGAPLISPPGEMVEGFVEMFAGQQAEQTTVGDGFPAMNEEEEADQFFSTTESSALERFLDNLASSSEINPLEFYHPNPPKLPHMFEMRTMGQQEHFGAPANAKPEPAAGFQMHPSQDLGGFGGGAKPAQQLQTPDTSVGQVQTKRVRDAPDASEPSANKRKLAKPLLSSTQKRLNHSHLEQKRRRLCKEAYDRCLRLVTNVDDYKNELIHVSADKGSKKKSRRRQLTKDGLPNLSKHAALQKISSEISKIQSKNEELRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB32</accession>
    <name>A0A1A0HB32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mating factor alpha precursor N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_40901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711608.1">
        <property type="nucleotide sequence ID" value="XM_018857326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB32"/>
    <dbReference type="STRING" id="869754.A0A1A0HB32"/>
    <dbReference type="GeneID" id="30030302"/>
    <dbReference type="OrthoDB" id="3766782at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007618">
        <property type="term" value="P:mating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008675">
        <property type="entry name" value="Mating_factor_alpha_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05436">
        <property type="entry name" value="MF_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291725" description="Mating factor alpha precursor N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="18"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Mating factor alpha precursor N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="20397" checksum="A3C36706FB0D0539" modified="2016-10-05" version="1">MKLSIPLIFGAITVASASPASEANLTINGTLIPQEAIISGLTIPEDVYPILNYENNTATVVFLNGTLLAEAKAYNSETKRDVAYPDREITKRDVEPHTWSWGDADHELASVKREAEAEAEADPWHWVRLKIGQPFFKRDAEGSADPWRWVSLTFAKPFFKRDAEASADPWHWVALKIGQPFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB47</accession>
    <name>A0A1A0HB47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MINDY deubiquitinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711623.1">
        <property type="nucleotide sequence ID" value="XM_018858837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB47"/>
    <dbReference type="STRING" id="869754.A0A1A0HB47"/>
    <dbReference type="GeneID" id="30031813"/>
    <dbReference type="OrthoDB" id="10261212at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="26"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="MINDY deubiquitinase" evidence="2">
        <location>
            <begin position="105"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="407" mass="44985" checksum="21C6BCFAFCEB3047" modified="2016-10-05" version="1">MIMRLLFQHGQNHTELRCQYGGGHEIRWSPYEQVLPILLQDTNGPCPLIALVNTLILTNDIEKRTVDLQEPAGPSRDLEPQAPPATAASSAQKEQQSVPGARRLNVAQLRSLLNLNIGGTVEVPQILSSLGDLLLDIPDTDSAVLNGLLESLPLLHTGLDVNPNLTTGGFDPEEMASQIFRVFGLNFVHGWCREPSGDADLDAVFARHQTFEALQEYLLTSAENTVQKTHIEAWVQDNASQLTAHGLRRIDEKMQPDSLAIFFRNNHFLTLYKAHNQDLYLLITDDAFLKHANYVWQSINSVSGGDDLYFAGDFTPLMDGADPGEAAGVDESLVFAKQLQEKEDIAYAQKLQLSYKKRAEKTPFSGAGEGPDPSLPQLEDKKMLLKKSSKGLSKEARSKKKDTCVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB50</accession>
    <name>A0A1A0HB50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_15483</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711864.1">
        <property type="nucleotide sequence ID" value="XM_018854602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB50"/>
    <dbReference type="STRING" id="869754.A0A1A0HB50"/>
    <dbReference type="GeneID" id="30027578"/>
    <dbReference type="OrthoDB" id="21123at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051376">
        <property type="entry name" value="CWC25_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="10">
        <location>
            <begin position="10"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="154"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="156"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="211" mass="24297" checksum="ADE87410553CF5D3" modified="2016-10-05" version="1" fragment="single">MPGDLNLKKSWNPALMKNQKKVWEREQQALAELQSIKQRQKEIAQEREKEHMIRLQYGSDPSSMPTKDKLELNKLGWMYNDGPKTTADDGSGFREVDEDFLARSDEIQLLVRGNKAVQPAVGSRFEKVAAVGRLAPSALLSDDPLLLIRREHFKKRADNRDRSPSRLHGEGSSRTLHDSRSRHRDSRERKEGRDRPGDRRGHGRDSRSHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB53</accession>
    <name>A0A1A0HB53_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_178533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711751.1">
        <property type="nucleotide sequence ID" value="XM_018854738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB53"/>
    <dbReference type="GeneID" id="30027714"/>
    <dbReference type="OrthoDB" id="10323670at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="147" mass="16237" checksum="D95A4833D00BB681" modified="2016-10-05" version="1">MSGNLILFDDRCSVKLCREPAPSVTKGSYQGAPSDPIPIVSHDQTSKSSSTIRTEPRAGSKGLSEEQKNLVGDDQEIKHTVPLFRSSDYKLVSNIFIGLMMFQYILIFFAIPFSEVDNRPAASVLLCLLGYLLVCSNNFLAIFRVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBQ0</accession>
    <name>A0A1A0HBQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_11928</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711920.1">
        <property type="nucleotide sequence ID" value="XM_018854128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBQ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBQ0"/>
    <dbReference type="GeneID" id="30027104"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990526">
        <property type="term" value="C:Ste12p-Dig1p-Dig2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990527">
        <property type="term" value="C:Tec1p-Ste12p-Dig1p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000220">
        <property type="term" value="P:regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052127">
        <property type="entry name" value="STE12_transcription_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427:SF1">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="111" mass="12149" checksum="9A6ACC8C0928B383" modified="2016-10-05" version="1">MADALERDIKKEKANQTPTSVALREPALLFNYVEDKTKSLYDQLSEHLSQPRFDTVKLELQSGLASPDANTSTLAALKLPSDSSCASTGYRKMPGDAAYLIGHTDDYPISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCV0</accession>
    <name>A0A1A0HCV0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Outer mitochondrial membrane cytochrome B5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_19731</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712327.1">
        <property type="nucleotide sequence ID" value="XM_018854885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCV0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCV0"/>
    <dbReference type="GeneID" id="30027861"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000002">
        <property type="entry name" value="Cytochrome b5 type B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050668">
        <property type="entry name" value="Cytochrome_b5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359">
        <property type="entry name" value="CYTOCHROME B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19359:SF95">
        <property type="entry name" value="CYTOCHROME B5 TYPE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="8735" checksum="F9E1137FB129065B" modified="2016-10-05" version="1" fragment="single">YTLAEVKQHGSPDDLWMILYNRVYDFTAFSAQHPGGAEVLFDCGGVDATEAFEDVGHSKNACEMLAPFLLGRVPKNDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD73</accession>
    <name>A0A1A0HD73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_10772</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712343.1">
        <property type="nucleotide sequence ID" value="XM_018853852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD73"/>
    <dbReference type="STRING" id="869754.A0A1A0HD73"/>
    <dbReference type="GeneID" id="30026828"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF317">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE ECM32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="2">
        <location>
            <begin position="192"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="2">
        <location>
            <begin position="323"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="3">
        <location>
            <begin position="404"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="655" mass="74025" checksum="DEED32007275F7C6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD87</accession>
    <name>A0A1A0HD87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Anp1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_77417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712358.1">
        <property type="nucleotide sequence ID" value="XM_018858697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD87"/>
    <dbReference type="STRING" id="869754.A0A1A0HD87"/>
    <dbReference type="GeneID" id="30031673"/>
    <dbReference type="OrthoDB" id="2405412at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140497">
        <property type="term" value="C:mannan polymerase II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF6">
        <property type="entry name" value="MANNAN POLYMERASE COMPLEXES SUBUNIT MNN9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41542" checksum="D79273B58EB545A6" modified="2016-10-05" version="1">MANPPPQIMRGIRRKPLVLVGAAAFFLAVYWLVFSASAPPQLHSALSTKYSYTKKSGGWFSLGSRDLPILVNLPKNHISHYDLNKLHAAPDKAHAAEVLILTPMSKFVPEYWANLNALTYDHKLVSLGFIFPRTAEGDHAMKQLEKHVKATQRGAGRFKKITMLRQDSPSLESQTEKARHALLVQKERRSMMALARNSLVFTTLAPATRWVMWMDADIVETPPSILEDMMAHNKPVLSANVYQRYTKDGQPAIRPYDFNNWVESEEGLRLAGEMPDDDIIVEGYSEIATFRPLMAHFYDEKGDASVEMPLDGVGGGAVLVNADVHRDGAMFPSFPFYHLIETEGFAKMAKRLGYEVFGLPNYLVYHYNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDG3</accession>
    <name>A0A1A0HDG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_14952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712511.1">
        <property type="nucleotide sequence ID" value="XM_018854559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDG3"/>
    <dbReference type="GeneID" id="30027535"/>
    <dbReference type="OrthoDB" id="4087523at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508825" description="Extracellular membrane protein CFEM domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="15363" checksum="BD9FC78BED6EBF7D" modified="2016-10-05" version="1" fragment="single">SRVIRLTLSHLLLLLLCIASLALAIESSVIENVSYKNLNPRPYDNCEANDLIKLLACCNDVLASLDDCKAGDLACECCALQSMDLTCYNLCPGNPSTNFLAVLFDDCSSLNDVNACNIPFKRVDGDRPASFRNRKATVDTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDL5</accession>
    <name>A0A1A0HDL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF775-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_10891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OPI10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712483.1">
        <property type="nucleotide sequence ID" value="XM_018853874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDL5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDL5"/>
    <dbReference type="GeneID" id="30026850"/>
    <dbReference type="OrthoDB" id="10248398at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048364">
        <property type="entry name" value="Hikeshi-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008493">
        <property type="entry name" value="Hikeshi-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031318">
        <property type="entry name" value="OPI10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925">
        <property type="entry name" value="HIKESHI FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12925:SF0">
        <property type="entry name" value="PROTEIN HIKESHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21057">
        <property type="entry name" value="Hikeshi-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05603">
        <property type="entry name" value="Hikeshi-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hikeshi-like N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Hikeshi-like C-terminal" evidence="3">
        <location>
            <begin position="158"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="210" mass="23096" checksum="DAE484142969C7F8" modified="2016-10-05" version="1">MFGAVCSGRPMQLAQQVEPTKYVFSIPNAGNISHVAVFMLPQTEFVDLSFTALVYFQLPALPEFKLLGGLNPNKPSSIFRLKNAGAGLRSSSHTLDEDDMMNDAETTVAGEETVLNIGISIEPTVEAEVLLQQERTRQKTITLPPSQAPAAPKAPLDIAVLANQIVKHAYNYLGGFIDATGKVPMKVFDNWWDKFRMKLSNNPNFLSELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDS7</accession>
    <name>A0A1A0HDS7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pyruvate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_39027</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712574.1">
        <property type="nucleotide sequence ID" value="XM_018857082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDS7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDS7"/>
    <dbReference type="GeneID" id="30030058"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="39"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="459"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="504"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="532"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="620" mass="68591" checksum="FC6289F329C30FA6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ3</accession>
    <name>A0A1A0HDZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_148292</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ELOF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712696.1">
        <property type="nucleotide sequence ID" value="XM_018854546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDZ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDZ3"/>
    <dbReference type="GeneID" id="30027522"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF0">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="81"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="122" mass="13601" checksum="E2DC825064C644EA" modified="2016-10-05" version="1">MGKRKTAAKPVKKVKQKLDISFRCLFCNHEKSVICTMDKRNGIGDLHCKICGQSFQTAINSLSHPVDIYSDWIDACEDLAEDNEAEKSAEEAGESVKGQYEYSDDDGAPPAKKTSEYSDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE27</accession>
    <name>A0A1A0HE27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">P-type Na(+) transporter</fullName>
            <ecNumber evidence="19">7.2.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">METBIDRAFT_31192</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) + ATP + H2O = K(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:75815"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Na(+)(in) + ATP + H2O = Na(+)(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:14634"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IID subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.3" evidence="19"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712762.1">
        <property type="nucleotide sequence ID" value="XM_018855800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE27"/>
    <dbReference type="STRING" id="869754.A0A1A0HE27"/>
    <dbReference type="GeneID" id="30028776"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008554">
        <property type="term" value="F:P-type sodium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000015">
        <property type="entry name" value="Sodium ion P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000039">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000047">
        <property type="entry name" value="Sodium P-type ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006414">
        <property type="entry name" value="P-type_ATPase_IID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01523">
        <property type="entry name" value="ATPase-IID_K-Na"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42861">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="12" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="345"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="811"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="890"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="987"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1022"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="24">
        <location>
            <begin position="37"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00035017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00035029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00049499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OBA22266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1085" mass="119602" checksum="FE3276262B092750" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE40</accession>
    <name>A0A1A0HE40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">GPI mannosyltransferase 1</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_31181</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. Required for cell wall integrity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712746.1">
        <property type="nucleotide sequence ID" value="XM_018855796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE40"/>
    <dbReference type="STRING" id="869754.A0A1A0HE40"/>
    <dbReference type="GeneID" id="30028772"/>
    <dbReference type="OrthoDB" id="1741594at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180041">
        <property type="term" value="F:dol-P-Man:GlcN-acyl-PI alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 13" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 13" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="413" mass="48430" checksum="C973BD4C1E44F5BF" modified="2016-10-05" version="1">MQLTLKHLFVTATLLRVGFFLFGLYQDNNFLVKYTDIDYLVFSDAARYVYDGLSPYKRETYRYTPLLAWMLVPNSWGGIFAHFGKFLFMLCDILTGILISRNLAHSKISHRKKLIFLSIWLLNPMVVTISTRGSSESILTFFIMLSVDLYLNERYVEAAFWLGLSIHFKIYPIIYLPGLMLNLATKSPPFPLFRNVPVLKWLNSINVKFLVHTLISLTAWNVVMYYIYGYEFIYHSYIYHFTRLDHRHNFSLYNVMLYYKSAYPDVVSLTEMNCWSWLGQVSGHIEKVAFLPQLFLSAIILPLLLAQKDIIACLFVQTFAFVLLNKVITSQYFIWFLIFLPQFLANSKLSSPLNLKRSSIMIVMWSFSQACWLFYAYKLEFLGESTFTMGLFCSSVFFYLTNCWVLGQFIEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE64</accession>
    <name>A0A1A0HE64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">POP1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_40082</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712776.1">
        <property type="nucleotide sequence ID" value="XM_018857226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE64"/>
    <dbReference type="STRING" id="869754.A0A1A0HE64"/>
    <dbReference type="GeneID" id="30030202"/>
    <dbReference type="OrthoDB" id="442863at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="5">
        <location>
            <begin position="41"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="6">
        <location>
            <begin position="490"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="145"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="750" mass="85064" checksum="811883CCE1F663CB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF38</accession>
    <name>A0A1A0HF38_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor CWC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_38726</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the 'nineteen complex' NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CWC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713112.1">
        <property type="nucleotide sequence ID" value="XM_018857039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF38"/>
    <dbReference type="STRING" id="869754.A0A1A0HF38"/>
    <dbReference type="GeneID" id="30030015"/>
    <dbReference type="OrthoDB" id="10251848at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045787">
        <property type="term" value="P:positive regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032297">
        <property type="entry name" value="Torus"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16131">
        <property type="entry name" value="Torus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="18">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="17">
        <location>
            <begin position="129"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="16">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA22616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="245" mass="27314" checksum="1E061A0972D0ECB0" modified="2016-10-05" version="1" fragment="single">MNPRKYARLQVDADAIPDDDKPPQSGHTFNLWYLKWAGGDSTARGTARRAFRVDVPRDAGATRGADGSSPICLFFARGCCYRGRKCPYLHRLPQAGDRRVPTQDCFGRDKAAADRDDTRGAGLLRRHNRTLYVAGAHVDDRVQARLHRQFLEFGAVDQIRVLAARQCAFVSFRLEAEAEFAREAMDGQTLDGTDVLTLRWANEDPDPRAQQQAQRAVEAEAVATVKRLLAGVAEPPQSKRRQAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF40</accession>
    <name>A0A1A0HF40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PNK3P-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_17530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712999.1">
        <property type="nucleotide sequence ID" value="XM_018854719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF40"/>
    <dbReference type="STRING" id="869754.A0A1A0HF40"/>
    <dbReference type="GeneID" id="30027695"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046403">
        <property type="term" value="F:polynucleotide 3'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006551">
        <property type="entry name" value="Polynucleotide_phosphatase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01664">
        <property type="entry name" value="DNA-3'-Pase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE/KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12083:SF9">
        <property type="entry name" value="BIFUNCTIONAL POLYNUCLEOTIDE PHOSPHATASE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="223" mass="25071" checksum="1F14202B1BB704E9" modified="2016-10-05" version="1" fragment="single">SILSPQWKIHGSHMVTRLPLASSFRCFYKDKLDSDSKKIRVAAFDMDDTLICTKSGIKFGRGPYDWKWRNDQILPALQKKVKDKNMVLVIFTNQSAVSVTELLAPHSKSYKNLSLKVGLISKALETGIGPTPVLFFASTGRPRQGVLARSSLETHFSHKKPELGMWSELERYIRRCFGEEYSVDLDLSYFVGDAAGRDGDHLAADKGFAENAGVPFFVPEDYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF50</accession>
    <name>A0A1A0HF50_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Auxin efflux carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31402</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713009.1">
        <property type="nucleotide sequence ID" value="XM_018855844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF50"/>
    <dbReference type="STRING" id="869754.A0A1A0HF50"/>
    <dbReference type="GeneID" id="30028820"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040254">
        <property type="entry name" value="Ecm3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274:SF1">
        <property type="entry name" value="AGL149CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31274">
        <property type="entry name" value="PROTEIN ECM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="431"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="544"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="181"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="214"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="574" mass="63400" checksum="F7493DD098F60DFA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFD1</accession>
    <name>A0A1A0HFD1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SAE2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_29437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713335.1">
        <property type="nucleotide sequence ID" value="XM_018855401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFD1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFD1"/>
    <dbReference type="GeneID" id="30028377"/>
    <dbReference type="OrthoDB" id="4083304at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="271"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="468" mass="53126" checksum="1939C19A5C344DE2" modified="2016-10-05" version="1">MDFHQLVKHSIANINDELSKILTLTTLFDSKPSEAQSAFYKRIVQEFNSKIHKIKSENTRLNQKNKVLADKVKLLESQLLYFQSKTDLLPLKARPSKSDGALRSEQDRESTDIFKLDKALDYQKPNLEILSSPIKGSEQDSAKRSLRKQEDADTKNDITSSQFNRLATQYSDASSQPEAVQFEAPKLRVLNSSLMKCHGSGKEAEHAQLQSSSPFKRLKGNDRESFEQSFKGSFASDDESEEGEITGLNRIVSDSQDEFEPLGNAGWSPSKKGNSTLGTRSSAQSKPKYPSHYTALQRVEFLRNYLRPKVADKNFTIDFSKNPITEKQWVPEDFTPNEKWRPPKNINPNLGVMTKAKEKMYDEFFEKAGLGRRSAGPAWDSDDEPGLAVEGAEWVRSQVMDKYLSPPGFMVGDFANTQEVEERKQARIEKDKDRIKRRFKSAISGGEFLFYEPVFNEFVKTGKYVNGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFD3</accession>
    <name>A0A1A0HFD3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_163160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713103.1">
        <property type="nucleotide sequence ID" value="XM_018854635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFD3"/>
    <dbReference type="GeneID" id="30027611"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="56" mass="6769" checksum="62FB598CF026FEB9" modified="2016-10-05" version="1">MSFRRIDHLYFPISWRSNVLYYKIEKPPVITSSHHLIAVYFCTDQPLSTKKKEKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFF4</accession>
    <name>A0A1A0HFF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mitochondrial ribosomal protein L51</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_9229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713360.1">
        <property type="nucleotide sequence ID" value="XM_018859338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFF4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFF4"/>
    <dbReference type="GeneID" id="30032313"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016712">
        <property type="entry name" value="Rbsml_bS1m-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28058">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP51, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28058:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11709">
        <property type="entry name" value="Mit_ribos_Mrp51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018156">
        <property type="entry name" value="MRPL51_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="363" mass="40415" checksum="A797AB66B2140D97" modified="2016-10-05" version="1">MNNQELFRLFKHSKLAQVALPLSKNVRGPSTSSPTHQIIYTPKSSALRSDYGMKTALPKQVGVSHIAFNDIDSAKSMPDVEKRAGPLYTRLRFQESEVVLKKSYNKANPLFAWEDSKSLQGIKSDMPESIATQLNMNRNASLLDVKKAMAENPEIYTNFKQWVLENSPESFMLKVPSKFDALLKEFFSTSKFVRRRENSLEDMIKPQDRSKASGARSSVQGTAGLSYLQRGRLINSPNGIKHGSIVPGRLLGEREATIGGFVANVNEKTTLLQANYAKNSPGKHPKQFVLPFKVNEAEITANGSVRLQADGVKVGSWMLRSDLDSDSDRYPASNPNFGSLPERSQKEFSFLENVLGLTPRSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM6</accession>
    <name>A0A1A0HFM6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713144.1">
        <property type="nucleotide sequence ID" value="XM_018859302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFM6"/>
    <dbReference type="GeneID" id="30032277"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291783" evidence="1">
        <location>
            <begin position="23"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="354" mass="41531" checksum="DF0DCBDC7BD3A61F" modified="2016-10-05" version="1">MLIILTIFALLLIFKWWLKKDSQTGFFVNAKIAPIGSDFRWEDCEPLRLRPFVGKISFNPLMGVRNMSQKREELFLIENTYLETTNRRKSHLGRFEHKMIHCNHSEMAQSAVREFYSMAVSFLANRYPQYFKLDSAKGTIHNLINDDVFPLSGRNRDVKYLLRVLTANIEEDVIIMLKDHPENEDAEYILRASLTGSPAGFDPSHNFDRPVSFIHNPVPQYEARLFSPMHRFFNKIASKDIWQRANWSIQTNRILFKLEDHHGRCGDDPEEQETQDIDFENACFMRCERQLITRLPQTKAVLMLVRTYLTPLKTIKEESHATEMAQAIESLPEDLALYKRRHVWGKAVCEYLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG16</accession>
    <name>A0A1A0HG16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713279.1">
        <property type="nucleotide sequence ID" value="XM_018856771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG16"/>
    <dbReference type="GeneID" id="30029747"/>
    <dbReference type="OrthoDB" id="4078100at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024274">
        <property type="entry name" value="APC9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12856">
        <property type="entry name" value="ANAPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="436" mass="48811" checksum="3B185AE14A25AAF2" modified="2016-10-05" version="1">MADEKNIVKLHIPRLRVPSHTLNEASYNSMPNVSQRTVSSSTSLLSTPSNNIRRVQQQMNAQARPSMARLSRAKAPDVCFAGVSSTATASKKLLNLQQLRRAQTNPRPFVLRKPATMAKYDYSAFVDCHTNSETGDVNIPPLVKDSQVRAWKSAEKISAEIIFENGSSSSSPNDTTDVLDEGDGNSLNEDARASVEGCDTDEAPSASVLLESIPQYTKGELKVILKNYRKLQNCASPPSYYELEDTEKKELWLYRQHQSVTQERAFTANHTLLGKRKVQVFHKKEFLHKLFGYSNNINQECTTNNTSYSASDNAFNAQKAFHEDKEEILGLLAEDKAFEEELKTAKEHLLALVSKPGNGPDAQNKVFDQPMDTLDLLDLTNSWLTSSYEKQSGHCDSEEASECEPDQHPEVIAYTMGLLKRRVRDAFDDHVDMPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGA0</accession>
    <name>A0A1A0HGA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_96745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713512.1">
        <property type="nucleotide sequence ID" value="XM_018859436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGA0"/>
    <dbReference type="GeneID" id="30032411"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="76" mass="8574" checksum="F755E502DAD30E63" modified="2016-10-05" version="1">MSSELLYTIVAIISVFTAKGKSSPIKLYRSILVLLLRQEPASISLSPLYIGSTIALHLRTDMILRQSFLWLQLIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGC2</accession>
    <name>A0A1A0HGC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_9360</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713521.1">
        <property type="nucleotide sequence ID" value="XM_018859367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGC2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGC2"/>
    <dbReference type="GeneID" id="30032342"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045009">
        <property type="term" value="C:chitosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="414"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="988"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1015"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1042"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="322"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1122"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1129"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1147" mass="127842" checksum="6CA9F487D1CDD843" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGL2</accession>
    <name>A0A1A0HGL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">mRNA m(6)A methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.348</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76212</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>an adenosine in mRNA + S-adenosyl-L-methionine = an N(6)-methyladenosine in mRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55584"/>
            <dbReference type="Rhea" id="RHEA-COMP:12414"/>
            <dbReference type="Rhea" id="RHEA-COMP:12417"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
            <dbReference type="EC" id="2.1.1.348"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.348" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713622.1">
        <property type="nucleotide sequence ID" value="XM_018858495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGL2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGL2"/>
    <dbReference type="GeneID" id="30031471"/>
    <dbReference type="OrthoDB" id="10262526at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001734">
        <property type="term" value="F:mRNA m(6)A methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902974">
        <property type="term" value="P:meiotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000221">
        <property type="term" value="P:negative regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12829:SF7">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="495" mass="56537" checksum="56D01E29C19F8299" modified="2016-10-05" version="1">MAFEESCAVFLPFIEFLSQRKEALLDDPYKGDIETIRGVFREVCNDEPVKEKAKDQATFRDYLAEFNVVSGSALVFNSETDKQLIWIKVANISILKDRLKNSEPYSALDPQTVVNCEVSAEKGFDHDFARLNKMLARPTAMVKLGVKRAQLITTQKPFYEVCEVLDHSQTLANLCLNLKSIGLGFNELPSNAKIHKVISSCKSLLTKEVLACVRNKIHFLPLLTNNTDLYLGNCSYLDTCHKLKNCRYLHFYTLNPSQANVETSQQNPLLAIDYTIGECHDTMLRKELPAQWINCDVRYLPFSILGKFAAIISDPAWDIHMSLPYGTCKDSELLSLPMRELQDEGVLMLWVTGRSIEIGRRALEQWGYTVSDEMIWIKLNQLRKTIVTGRTGHWLNHSKEHLLVGVKGNPVWLNRKIDIDFIVSGTRETLRKPDELYGIVERLVGIHARKLEIFGRDNNIRPGWITIGNQLTGTSIYEEEVKQKYNRYQSASKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGM1</accession>
    <name>A0A1A0HGM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_96742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713508.1">
        <property type="nucleotide sequence ID" value="XM_018859435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGM1"/>
    <dbReference type="GeneID" id="30032410"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="91" mass="10792" checksum="DA34CEA0B9D7DDFF" modified="2016-10-05" version="1">MRRWTISNRPKTSECNHRAQLLCLHNLTITIVYCFSPSTWEYMQTLSPGSLHIFSGLQLRKIRPSVLYLESCTRREAWHLKKQLKLFLSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS4</accession>
    <name>A0A1A0HGS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AIP3-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_228380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713677.1">
        <property type="nucleotide sequence ID" value="XM_018855203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS4"/>
    <dbReference type="GeneID" id="30028179"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005519">
        <property type="term" value="F:cytoskeletal regulatory protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1540">
        <property type="entry name" value="Actin interacting protein 3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022782">
        <property type="entry name" value="AIP3-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005613">
        <property type="entry name" value="AIP3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056279">
        <property type="entry name" value="Aip3p_Bud6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051825">
        <property type="entry name" value="SRCIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741:SF10">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN CG32809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22741">
        <property type="entry name" value="P140CAP/SNIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03915">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23153">
        <property type="entry name" value="Aip3p_Bud6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00806">
        <property type="entry name" value="AIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Actin interacting protein 3 C-terminal" evidence="4">
        <location>
            <begin position="252"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="660"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="663"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="683" mass="76281" checksum="BBB9FC2D69F2A258" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGY9</accession>
    <name>A0A1A0HGY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome c oxidase subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cytochrome c oxidase polypeptide VIa</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_76218</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase subunit 6A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713628.1">
        <property type="nucleotide sequence ID" value="XM_018858497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGY9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGY9"/>
    <dbReference type="GeneID" id="30031473"/>
    <dbReference type="OrthoDB" id="5947505at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.95.10:FF:000001">
        <property type="entry name" value="Cytochrome c oxidase subunit 6A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.95.10">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001349">
        <property type="entry name" value="Cyt_c_oxidase_su6a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018507">
        <property type="entry name" value="Cyt_c_oxidase_su6a_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036418">
        <property type="entry name" value="Cyt_c_oxidase_su6a_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE VIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11504:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02046">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81411">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01329">
        <property type="entry name" value="COX6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="133" mass="15925" checksum="574417F24ABBF330" modified="2016-10-05" version="1">MFRNIAQRSFQRFNSHGHVSKLIKEDAFKNLYNKEAGEAFKKAQYEKAHHSEGITELWKKITFYVALPAVLLTAIPILKTELEHAKHREHLRHLSDEEWPVQYEYQNLRQKKFFWGDGDKTLFWNSDVNRHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH04</accession>
    <name>A0A1A0HH04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Interdomain Rrm packing contributes To Rna recognition in the Rna15, Hrp1, anchor Rna 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_18975</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713940.1">
        <property type="nucleotide sequence ID" value="XM_018854826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH04"/>
    <dbReference type="STRING" id="869754.A0A1A0HH04"/>
    <dbReference type="GeneID" id="30027802"/>
    <dbReference type="OrthoDB" id="1875751at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000025">
        <property type="entry name" value="RNA-binding protein Musashi homolog 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032:SF6">
        <property type="entry name" value="RNA-BINDING (RRM_RBD_RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48032">
        <property type="entry name" value="RNA-BINDING PROTEIN MUSASHI HOMOLOG RBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="4"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="88"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="166" mass="18834" checksum="9B37E52922C55103" modified="2016-10-05" version="1" fragment="single">RDSGKMFIGGLNWDTTEDGMVAYFSKYGEILDYTIMKDSNTGRSRGFGFLTFKDPRAVDEVIKKDHILDGKLIDPKRAIAREEQDKIGKIFVGGIDPLVNEKDFNDFFSQFGRIIDAQLMIDKDTGRSRGFGFITYDSPEAVDKVCLNKYLTLKGKAMEVKRAEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH51</accession>
    <name>A0A1A0HH51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_104047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713806.1">
        <property type="nucleotide sequence ID" value="XM_018853759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH51"/>
    <dbReference type="GeneID" id="30026735"/>
    <dbReference type="OrthoDB" id="8960535at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.50">
        <property type="entry name" value="Insect antifreeze protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="135" mass="16149" checksum="189614D0F311C52B" modified="2016-10-05" version="1">MEPVPCFCFSYHSNIIVVSYTIVVLYTIVVFNIIEYHYNRCLQYNRCVQYNRCVQYNRCFQYNRCGQYNRCVQYNSCFQYNRCGQYNRCVQYNRCAQYNNCFQYNRCANIIVVSNVVIVVSNVLLEAEIISVALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH86</accession>
    <name>A0A1A0HH86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_76541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714025.1">
        <property type="nucleotide sequence ID" value="XM_018858547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH86"/>
    <dbReference type="STRING" id="869754.A0A1A0HH86"/>
    <dbReference type="GeneID" id="30031523"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16980">
        <property type="entry name" value="VHS_Lsb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045007">
        <property type="entry name" value="LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789:SF1">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="2">
        <location>
            <begin position="21"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="462" mass="50322" checksum="E0B743D84DEF3B13" modified="2016-10-05" version="1">MPIFGEKPFTSITVKVNQLCTLNRNADLEDDSMELYVPDLVSLIKLQSLGAVEAARAIRKKIKYGNTPAELVLALDLLELLVLNAGSKIGQAIAADDKLGELLRAVASGLARSGLGGPYDGLVVRRVRELARGWRHEFQELPGYGNLAGLYKAMPRSSDKNDRSSDRNDRSSGKNDRSGARHVPPPRPQTKSPYAAKQKKKKSKRKRGVVYADEQYQIPQINYRAEAPKIRTTIADCHTHTTALDNALLALARGSDPMDDAKAAAEFERCRKIRRKVLRYLQYVGAGDGSHKSKEVLELDDEFLGSLLVANDQLVASFHRFDAMCGYTPENPAQYHQDDRRDDASVSSGESYYTSDSDDESAGDEIDDGESATGADDIGESAAGAGTENDAIAGRLRRLDVNDAGSDTDDAAGLAVPVHPRDRRQALARPQPVARQDTAVSTESGDPFGDRNEVSKGASVYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHK6</accession>
    <name>A0A1A0HHK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear rim protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_38036</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Member of a perinuclear network that controls recombination at multiple loci to maintain genome stability. Required for rDNA repeat stability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NUR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713843.1">
        <property type="nucleotide sequence ID" value="XM_018856948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHK6"/>
    <dbReference type="GeneID" id="30029924"/>
    <dbReference type="OrthoDB" id="3363151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018819">
        <property type="entry name" value="Nur1/Mug154"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28293:SF1">
        <property type="entry name" value="NUCLEAR RIM PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="145" mass="16668" checksum="082F61E419B4EA3D" modified="2016-10-05" version="1" fragment="single">MARKRIIRKQPLLEKIRSYPLDLLLSIHEQRLSIDWDDHVPKCLPVGSALTFIFLLLCKAREYYISVKDKRDNQLFSSDYSTYQQVVSRAVNGNGIHTAPVAVPKFQNESLAQTLLWIVNGAMIILFIISALNSANVYLLPYRNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHL6</accession>
    <name>A0A1A0HHL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exocyst complex component SEC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_104701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the SEC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713853.1">
        <property type="nucleotide sequence ID" value="XM_018853776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHL6"/>
    <dbReference type="GeneID" id="30026752"/>
    <dbReference type="OrthoDB" id="10267033at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.30">
        <property type="entry name" value="Exocyst complex subunit Sec15 C-terminal domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007225">
        <property type="entry name" value="EXOC6/Sec15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046361">
        <property type="entry name" value="EXOC6/Sec15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042045">
        <property type="entry name" value="EXOC6/Sec15_C_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048359">
        <property type="entry name" value="EXOC6_Sec15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12702">
        <property type="entry name" value="SEC15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20651">
        <property type="entry name" value="EXOC6_Sec15_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04091">
        <property type="entry name" value="Sec15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025007">
        <property type="entry name" value="Sec15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC6/Sec15 N-terminal" evidence="7">
        <location>
            <begin position="69"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex subunit EXOC6/Sec15 C-terminal" evidence="6">
        <location>
            <begin position="444"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR025007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="860" mass="98888" checksum="3D897BDA3080ABF2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHY4</accession>
    <name>A0A1A0HHY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAP-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_109185</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the nucleosome assembly protein (NAP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714101.1">
        <property type="nucleotide sequence ID" value="XM_018853882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHY4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHY4"/>
    <dbReference type="GeneID" id="30026858"/>
    <dbReference type="OrthoDB" id="27325at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032174">
        <property type="term" value="C:cellular bud neck septin collar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000511">
        <property type="term" value="F:H2A-H2B histone complex chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098841">
        <property type="term" value="P:protein localization to cell division site after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.90:FF:000003">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1500:FF:000001">
        <property type="entry name" value="Nucleosome assembly protein 1-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.90">
        <property type="entry name" value="Nucleosome assembly protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002164">
        <property type="entry name" value="NAP_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00956">
        <property type="entry name" value="NAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="147"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="295"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="346"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="364"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="393"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="404" mass="46458" checksum="B7FE049CD60EE8E8" modified="2016-10-05" version="1">MSEPIRKSSDISKAPTPQNTPSSVANSYLKQQPTVSTINEEEENALSKQLANNPALLSMIQGKLGTLVGQQSGYIEQLPKQVKKRVLGLKAIQQLQMKMEAEFQKELLELEKKYFKKYEPLYEKRMAIINGEQEPTAAEIEVGEEIEAEHKDPEDQDADDQSDAEGDDEEISGVPNFWLTALENLNTVSETITERDSEVLQYLRDIRMEYLQTPGFELIFEFNQNPYLSNQVLTKTYHYQAELGYSGDFVYDHADGCEINWKSKENNVTINIERRKQRNKNTKQTRTIEKLTPSESFFNFFDPPKPPSADSDEEDQEDDENDEDADLEERLELDYQLGEEIKDRLIPRAIDWFTGDAVGIGYPDDFEEDEEEFDSEDENDDDDDDDDDINETGPKENPPECKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIC8</accession>
    <name>A0A1A0HIC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714241.1">
        <property type="nucleotide sequence ID" value="XM_018859501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIC8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIC8"/>
    <dbReference type="GeneID" id="30032476"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF23">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ICT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="105"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="487" mass="54515" checksum="EAC68CF4B768A068" modified="2016-10-05" version="1">MLCSVRTLGTARARGSAPGAAYQPNRRVVKKLLFVQQCAMWLRSLRPGHLATLQRQLVEWLFPAGAAENQGVARDFVQTPVDARGSVVNSVVFLVQNDKAAETRHVVFVHGYGAALGCFARNFHVINRFKGLRHNYKVHFLDNLSFGLSSNPRVASLDYWRPVPRVDFLTMHDAAPTVKAGLHRKYYKLIAGYEVDAHRFGRYKAAVTPVLRDLEAYYTDALEAWRVNARIGRIHFLVGHSFGGYWCGSYALRHPDSVRHLVLLSPVGVERHAAAVTAPAPRGLGPIAPSLDPASPRFLSRWPILAARAVRHWYYVQPYLPRLLRLMGPWGVARYYDMWSGKLFAVNGVIARLGGAAVLTSSNQLRYGTNTECRLLIEYLYNSVTAGSRSDIHIKYLLTPATDSKWPLMDKFARASGATLAKFRTHVVYGQYDYMNSEAGEKMVAEMNGRPAVDARFHVVPEAGHNLYLQNPFGTNALLERLVREAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIN4</accession>
    <name>A0A1A0HIN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714228.1">
        <property type="nucleotide sequence ID" value="XM_018855567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIN4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIN4"/>
    <dbReference type="GeneID" id="30028543"/>
    <dbReference type="OrthoDB" id="4026618at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="329" mass="36079" checksum="1B072C50F9471E46" modified="2016-10-05" version="1" fragment="single">MANIFLASNVWAIFIEHNITMSTMNELILTSLTVRPEVYFSVINSNLFTVLGLFKNEGNINFTSSSSRTTGVRITGEEFENLGNVILNSLSNEAFSEFHITLLGSFQNTGNIYFGIQGGSYETAPFSVTSVTEWYNTGIMVFAATYGMDVRLDFECRSLSNELTSIVNDGTVCLNNTLWPVKTTIEGIGCITLGSGGQLDLQYSQRTYAIAAAQTVYLASFDSILKVTGWGLFEGNIPVIKIAGFGNSNLIQLHTYSVNGFRYSLTTGMLTVRVGDIHEVNFDIGTGYIMPLFRLTSSAIYYRGNPPQSPPEICFCATTFPTAPRALVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIN5</accession>
    <name>A0A1A0HIN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pescadillo homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nucleolar protein 7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">NOP7</name>
        <name evidence="8" type="ORF">METBIDRAFT_30243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the pescadillo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714347.1">
        <property type="nucleotide sequence ID" value="XM_018855582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIN5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIN5"/>
    <dbReference type="GeneID" id="30028558"/>
    <dbReference type="OrthoDB" id="10264910at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000067">
        <property type="entry name" value="Pescadillo homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03028">
        <property type="entry name" value="Pescadillo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="346"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="284"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="449"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="557"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="294"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="578"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="589" mass="66910" checksum="383CCCA3AFF6644D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ92</accession>
    <name>A0A1A0HJ92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Clavaminate synthase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30276</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714389.1">
        <property type="nucleotide sequence ID" value="XM_018855596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ92"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ92"/>
    <dbReference type="GeneID" id="30028572"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044283">
        <property type="term" value="P:small molecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000051">
        <property type="entry name" value="Clavaminate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="3">
        <location>
            <begin position="171"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="37978" checksum="B1EB920C0A18C587" modified="2016-10-05" version="1">MANNSNPLQIVDITDFNRNTAAELLNAATSQGFLFLEGHGFSAKEVSDLFALSKEFFDLPAEYKRKFPIDESNHGYTDHGGENLDPTKQKRGDPKEALNFCSLNFKTGTSSKEIPGWFKDDPSREKLLTSTISKLYNLSIKILQILALGLDIEDTEVDGTDWFTSRYDPEKASGTTFRFLHYPGQKSLDPESTIRAGAHTDYGSMTLLFQQEGQEGLEIYSPISKKWEAVPFVPSSFPEAKGQAPPIIVNIADQLSYWTGGLLKSTIHRVKFPAEAQRSGKDRYSIVFFSHPNDATLLGPVPSAIVRDIKHRGANKDTVPITAKQHLDKRLAATYGWSQDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJG8</accession>
    <name>A0A1A0HJG8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714464.1">
        <property type="nucleotide sequence ID" value="XM_018855620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJG8"/>
    <dbReference type="GeneID" id="30028596"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8585" checksum="E5A380FBB0C20B85" modified="2016-10-05" version="1" fragment="single">MSDILVSNISDDGWKVQGRKRRNSRKRPERSVTMATTPGLILTDQKGSLGSYPSMKQPSSNSNPFLVLAEISEEDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJH8</accession>
    <name>A0A1A0HJH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitochondrial ribosomal protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_65582</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial ribosome (mitoribosome) assembly factor. Binds at the interface of the head and body domains of the mitochondrial small ribosomal subunit (mt-SSU), occluding the mRNA channel and preventing compaction of the head domain towards the body. Probable inactive methyltransferase: retains the characteristic folding and ability to bind S-adenosyl-L-methionine, but it probably lost its methyltransferase activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714523.1">
        <property type="nucleotide sequence ID" value="XM_018858354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJH8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJH8"/>
    <dbReference type="GeneID" id="30031330"/>
    <dbReference type="OrthoDB" id="421327at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052571">
        <property type="entry name" value="Mt_RNA_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015324">
        <property type="entry name" value="Ribosomal_Rsm22-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016522">
        <property type="entry name" value="RSM22_mit_bud"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13184:SF5">
        <property type="entry name" value="METHYLTRANSFERASE-LIKE PROTEIN 17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09243">
        <property type="entry name" value="Rsm22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007797">
        <property type="entry name" value="RSM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="263"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="766" mass="85538" checksum="B9C3BC17E38DB21A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJI9</accession>
    <name>A0A1A0HJI9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sas10 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_35457</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714656.1">
        <property type="nucleotide sequence ID" value="XM_018856610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJI9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJI9"/>
    <dbReference type="GeneID" id="30029586"/>
    <dbReference type="OrthoDB" id="1924577at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018972">
        <property type="entry name" value="Sas10_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF8">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09368">
        <property type="entry name" value="Sas10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sas10 C-terminal" evidence="5">
        <location>
            <begin position="481"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="26"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="254"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="491"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="269"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="556" mass="61438" checksum="42BCDFEB0D774A58" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJK1</accession>
    <name>A0A1A0HJK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ras-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_35952</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Golgi apparatus membrane</location>
            <topology evidence="7">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714671.1">
        <property type="nucleotide sequence ID" value="XM_018856674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJK1"/>
    <dbReference type="GeneID" id="30029650"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01868">
        <property type="entry name" value="Rab11_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000067">
        <property type="entry name" value="ras-related protein RABA1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050209">
        <property type="entry name" value="Rab_GTPases_membrane_traffic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47979">
        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24453" checksum="EE5F836F66344237" modified="2016-10-05" version="1">MSDNTDDYSYDYEYLYKIVLIGDSGVGKSNLLSRFTRDEFNLESRSTIGVEFATRTLEIDGKRVKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKTESYESVSRWLKELKDHADANIVIELVGNKSDLDHLRAVPTDEAKSFATENSLLFTEASALSADNVDLSFHQLLKNIYEMISKHQLDTNENALAAKPRSGPTISLTPAPQEKSSKNSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK06</accession>
    <name>A0A1A0HK06_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714836.1">
        <property type="nucleotide sequence ID" value="XM_018853782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK06"/>
    <dbReference type="GeneID" id="30026758"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="241" mass="25748" checksum="48044995362CB2A7" modified="2016-10-05" version="1">MTLHLHVARAAGTCGLYPQDKTALGQKTREPVLACKNISAAGLGVHACCLHAALARASRCGRLAEGVQKAAHRGRPCIATISLRRTGPLFAAGALIPDTAAPSVNKCCTPGDRAQRMGCRGVGLDFLDLAVVGVAVFEFAIEFAIFDFAIFDFAIQFAVFEFAIQFAIKFAIEFAIFDLAIQFAIFDFAIQFAVFDFAIQFAIFDLAVQFAIFDLAVFGVAVCGVAAFEFVVGVADGICRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK64</accession>
    <name>A0A1A0HK64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714674.1">
        <property type="nucleotide sequence ID" value="XM_018856881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK64"/>
    <dbReference type="STRING" id="869754.A0A1A0HK64"/>
    <dbReference type="GeneID" id="30029857"/>
    <dbReference type="OrthoDB" id="10005898at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF25">
        <property type="entry name" value="MICROBODY (PEROXISOME) PROLIFERATION PROTEIN PEROXIN 11C (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="305" mass="34362" checksum="983BBFACD02F99DC" modified="2016-10-05" version="1">MGTLVLGAQPRQNIPITVLLKLNKALKKSLFSRNFYLQINDKVLSQTATLDSTLYFICYFTLLLSAVLNNKHKILAFVKGQNARLIKVMGSFLPPKAGRVLSPEQPDASASGNEKSEPDRASENAAQETPSQLAVHLKAVSSYISDVRIFNRLVEAIKYMPWIIDEFSALANPVLAVPRTDRLVNFAQAINCLTLELFENVGWLSDHNWIGTSDNNYWCIESYIWSSRVWGAYIVVEMIELFRRHPASRRGKDWKISLFKQAVQLPLVLHWSLYDGCLSPFWVGLCGTGASWFGFKSVMESIDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK65</accession>
    <name>A0A1A0HK65_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_34960</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714896.1">
        <property type="nucleotide sequence ID" value="XM_018856546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK65"/>
    <dbReference type="STRING" id="869754.A0A1A0HK65"/>
    <dbReference type="GeneID" id="30029522"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="350"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="36"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="135"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="291"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="382" mass="41758" checksum="6415A1A5B5836088" modified="2016-10-05" version="1">MPQEPPSISAAVPGPRPSSAAPAPPDAKVIDKHSVEYVVRSGVAGGLSGSAAKTLIAPLDRIKILFQTSSPEFVQFRGLLGGLLLAGNRICAAEGPAGLFHGHLVTLLRVFPYAAIKFVAYEQIRSMLIPNDRHETAARRFMAGLLLGLASVFLTYPLDLVRVRMAFDTTPMAPGPARRRSLWAIVRAVYAEHPPRRPLDPRWLRLTRQKVPPALVPLSNFYRGFGPTILGMVPYAGVSFYVHDAIHDLFRLGLLAAYLVLRPAAPPSGPRVVKVKDRQQHVSSRDSRVPLKAYAQLAAGGFAGMFAQTAAYPFEVIRRRMQVGGAVDLGRFMTFRTTTRLIFAENGVRGFFVGLSIGYIKVIPMTACAFYVYERAKLLLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKF6</accession>
    <name>A0A1A0HKF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714769.1">
        <property type="nucleotide sequence ID" value="XM_018858642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKF6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKF6"/>
    <dbReference type="GeneID" id="30031618"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043224">
        <property type="term" value="C:nuclear SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010828">
        <property type="term" value="P:positive regulation of D-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000001">
        <property type="entry name" value="Cullin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000029">
        <property type="entry name" value="Cullin homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cullin family profile" evidence="6">
        <location>
            <begin position="429"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="785" mass="90742" checksum="9831437B7CC0B162" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKH5</accession>
    <name>A0A1A0HKH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenine nucleotide alpha hydrolases-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714972.1">
        <property type="nucleotide sequence ID" value="XM_018856427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKH5"/>
    <dbReference type="STRING" id="869754.A0A1A0HKH5"/>
    <dbReference type="GeneID" id="30029403"/>
    <dbReference type="OrthoDB" id="992776at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23659">
        <property type="entry name" value="USP_At3g01520-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100">
        <property type="entry name" value="IMP2'P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46100:SF4">
        <property type="entry name" value="USPA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="296"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="467" mass="52303" checksum="B2EB4B3CF87BF644" modified="2016-10-05" version="1">MASRLSKLEAEKLAIIQQSMSNRGRSLSPSGNNSRSSSALADDFNNREMKWSIINHDPLERIQGENSTGAAAGEEMWDDENSEEEHISDDYEGEKFEYNGTGNILPNYACHDEKMTEISSILEHSNLNDQETIKKLEELTANERAVANAKKIGSNVDNDVVAKLAENKHALTGQDKLDLNKSDQDAESRRQKLEAYQAYRRKIIHQENDNLRGRQSPSSKKSDDTTTEGSDASEFMIPYTSAVEDKLDSEFAFQLNETIKEGEVDSNISQSRVIQTITRGNFFQLINPQIKPKMFLLCMDFSPESIFALEWCLGTVLVDGSVLFIVCVIEDNDTNHQLKGNTLNESARESARLNMLNRAKQQVLNLLKLTKLQLHIVIEIIHHPIPRHLILECIDNLQPTLVVVGSKGLSAIKGVLLGSLSNYLVTKSSVPVMVVREKLKKINRFKSGSSIFSNNIKPLTLSEARVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKJ5</accession>
    <name>A0A1A0HKJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_132925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714893.1">
        <property type="nucleotide sequence ID" value="XM_018854335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKJ5"/>
    <dbReference type="GeneID" id="30027311"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="65" mass="7682" checksum="5C7C148046DC728E" modified="2016-10-05" version="1">MSRRPRFSSTGSPGRAWWNRQCLLVFGVFFVVVSFCFCCFCCFLSFCCFLCFWCFFVFVGFVVFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKZ4</accession>
    <name>A0A1A0HKZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Chromatin remodelling complex ATPase chain ISW1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_134373</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714959.1">
        <property type="nucleotide sequence ID" value="XM_018854361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HKZ4"/>
    <dbReference type="GeneID" id="30027337"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036436">
        <property type="term" value="C:Isw1a complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036437">
        <property type="term" value="C:Isw1b complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030874">
        <property type="term" value="C:nucleolar chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903895">
        <property type="term" value="P:negative regulation of IRE1-mediated unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046832">
        <property type="term" value="P:negative regulation of RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001178">
        <property type="term" value="P:regulation of transcriptional start site selection at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.30:FF:000003">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase ISW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="161"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="462"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1009"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1012"/>
            <end position="1023"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA24478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1038" mass="119883" checksum="6C81D7E701520EEA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ08</accession>
    <name>A0A1A0GZ08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_80176</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709306.1">
        <property type="nucleotide sequence ID" value="XM_018859186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ08"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ08"/>
    <dbReference type="GeneID" id="30032162"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="254"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1100"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1190"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1057"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="1112"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1190"/>
            <end position="1202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA17009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1209" mass="136442" checksum="C0DB16DB4FCF22F4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H294</accession>
    <name>A0A1A0H294_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_47861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709468.1">
        <property type="nucleotide sequence ID" value="XM_018858233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H294"/>
    <dbReference type="GeneID" id="30031209"/>
    <dbReference type="OrthoDB" id="6285886at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.50">
        <property type="entry name" value="Insect antifreeze protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016133">
        <property type="entry name" value="Insect_cyst_antifreeze_prot"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51156">
        <property type="entry name" value="Insect cysteine-rich antifreeze protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="92" mass="11038" checksum="E7B101F6E8EEDA88" modified="2016-10-05" version="1">MDLRRCIDLRLRGLRNCMDLSNCMDLRNCMDLRNCMDLRRCIDLRLRGLKELNCMDLSNCMDLRDCMDLRNCMYLSNCMDLRNCMDLRLPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4E8</accession>
    <name>A0A1A0H4E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Galactose oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709484.1">
        <property type="nucleotide sequence ID" value="XM_018856341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4E8"/>
    <dbReference type="STRING" id="869754.A0A1A0H4E8"/>
    <dbReference type="GeneID" id="30029317"/>
    <dbReference type="OrthoDB" id="4447at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025183">
        <property type="entry name" value="DUF4110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052588">
        <property type="entry name" value="Kelch_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46063:SF1">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13422">
        <property type="entry name" value="DUF4110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4110" evidence="2">
        <location>
            <begin position="544"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="488"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="494"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA18949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="632" mass="73030" checksum="C3DE7D81D1523CED" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4I4</accession>
    <name>A0A1A0H4I4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_84747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709486.1">
        <property type="nucleotide sequence ID" value="XM_018859241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4I4"/>
    <dbReference type="GeneID" id="30032216"/>
    <dbReference type="OrthoDB" id="4082198at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="359" mass="40874" checksum="EE336AA68307B7FB" modified="2016-10-05" version="1">MHCDHHIFGLFATHWHELTKKHKKPGALNKTADLDTIKEGGESEGFDSILWSKCFKICEKTHGESLPCFSMADFNTFLTSHTQNFVEKDCGALKLYNDLFGKQNLKDILFKKNEESSTVGKRFVIKLDLLISGSSELGAFLDEAFIIRASHRVALFLEEEFQNPLNPVFCITFRKREAYNAIKYYINDVNYSCLSIPTLLRKIKTTLEIVEELKAQIHTEGSSIVIQDLRDEVSEAVKNSQNIQRSVTQVIHAIDSGSDLLRTLSAWANYECSLTEVYESLKARHDMFMATVPFDRIQKIKYLCDYLTTASKKIFETVKEIEENLSDSISLSLSLDTHSFAANSKEKFLVVDHSNGSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5D0</accession>
    <name>A0A1A0H5D0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="3 11">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_46908</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="3 11"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709821.1">
        <property type="nucleotide sequence ID" value="XM_018858100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5D0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5D0"/>
    <dbReference type="GeneID" id="30031076"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000015">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000111">
        <property type="entry name" value="Glucose-6-phosphate 1-dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 11" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="6 11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="12">
        <location>
            <begin position="13"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="196"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="503" mass="57315" checksum="8448ED1E2037951B" modified="2016-10-05" version="1">MHSDSFGEHVSIVVFGASGDLAKKKTFPALFGLYREGQLPAHVNIIGYARSDLAIDDLRDRIKQNLKLDDEKSSSKVDSFLQLISYISGPYDKPEGYEKIEQAIQEYDLKNKVEKSERLFYLALPPSVFTNVGAHIKKYCYPKENGRLRLIVEKPFGHDLESARQMQKDLAPLFTEEELYRIDHYLGKEMVKNLLFLRFGNAMFSGIWDSKHICSVQISFKEAFGTEGRGGYFDDIGIVRDVMQNHLLQVLTLLTMERPVSFDPEAVRDEKVKVLKAFEEVKKENILIGQYGKSEDGEKPGYLDDETVKGESKCVTYAAMGLSIHNERWDGVPFVLRAGKALNESKVEVRIQFKDVAKGIFTDIPRNELVIRIQPNEAVYMKINSKIPGIANELSVTELDLTYASRFSKEFWVPAAYEALIRDCFLGNHANFVRDDELDVSWQLFTPLLKYIESEDAPELHIYPYGSKGPKPLKSFLAKHGYAFQTEGSYQWPLTKPNVKGKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F2</accession>
    <name>A0A1A0H5F2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SPIN90/Ldb17 leucine-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46564</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709798.1">
        <property type="nucleotide sequence ID" value="XM_018858054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F2"/>
    <dbReference type="GeneID" id="30031030"/>
    <dbReference type="OrthoDB" id="445362at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030125">
        <property type="entry name" value="SPIN90/Ldb17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018556">
        <property type="entry name" value="SPIN90/Ldb17_LRD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357:SF1">
        <property type="entry name" value="NCK-INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13357">
        <property type="entry name" value="SH3 ADAPTER PROTEIN SPIN90 NCK INTERACTING PROTEIN WITH SH3 DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09431">
        <property type="entry name" value="SPIN90_LRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SPIN90/Ldb17 leucine-rich" evidence="2">
        <location>
            <begin position="255"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="635" mass="72041" checksum="A70F44807B6B426F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5L4</accession>
    <name>A0A1A0H5L4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Non-structural maintenance of chromosomes element 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_60997</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SMC5-SMC6 complex, that promotes sister chromatid alignment after DNA damage and facilitates double-stranded DNA breaks (DSBs) repair via homologous recombination between sister chromatids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the SMC5-SMC6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NSE4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709916.1">
        <property type="nucleotide sequence ID" value="XM_018858313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5L4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5L4"/>
    <dbReference type="GeneID" id="30031289"/>
    <dbReference type="OrthoDB" id="361242at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027786">
        <property type="entry name" value="Nse4/EID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014854">
        <property type="entry name" value="Nse4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16140:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08743">
        <property type="entry name" value="Nse4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-structural maintenance of chromosome element 4 C-terminal" evidence="9">
        <location>
            <begin position="200"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="34619" checksum="B824DEF6BB6EF4C5" modified="2016-10-05" version="1" fragment="single">MLKLESLAHKGEAGQLVLQRIKELETLFSSVTNSEIKELSMYMKDSEALRKTTEFASTNAEHIRFGDLGGTLPLEHYLKAVKQYMNPDIAHEQQGEPVGRSAEDMNERFNSFDWDKLGNWFYLRGRVPVPSHFLCGPLATQRRRIATRTRTIDDTVSSAAITTARKVDASELENDPEQSTSHMVRALYNVIREKDPETKVSLYRLFINPHSFSQSVENLFYISFLARDGRIRIDEGPDNVPYVEIPDKDQDDDEDQDEESLLLHYIASLDQASWNWLIDRYNITDSYVPHRTQEEDRISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5P1</accession>
    <name>A0A1A0H5P1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46962</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709883.1">
        <property type="nucleotide sequence ID" value="XM_018858106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5P1"/>
    <dbReference type="GeneID" id="30031082"/>
    <dbReference type="OrthoDB" id="4022411at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990879">
        <property type="term" value="C:CST complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024222">
        <property type="entry name" value="Ten1_fungal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12658">
        <property type="entry name" value="Ten1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="135" mass="14485" checksum="5C91DCE211C6BBE8" modified="2016-10-05" version="1">MTVTILDPAKLSLFYPKASDVTPQKIRVISQAVDYDISSALLAVQRLPNLGPLHEEINLDGLDAENTEPQLAHVDLSKTNHASSSLYIPPGTIVSVVGFYNGSIITAVECIPLSEQVLLGKYIDTLAAVGTLKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5R3</accession>
    <name>A0A1A0H5R3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit OST5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33484</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the OST5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709827.1">
        <property type="nucleotide sequence ID" value="XM_018856307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5R3"/>
    <dbReference type="GeneID" id="30029283"/>
    <dbReference type="OrthoDB" id="4090047at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007915">
        <property type="entry name" value="TMEM258/Ost5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05251">
        <property type="entry name" value="Ost5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="88" mass="9790" checksum="BD30A537F375A5A9" modified="2016-10-05" version="1">MSFIENKIKYIDLITDFQQNSEQILPSKLSQYQLLITLILALLSFASVALTLINRKANFATYLTSASVASVSIALTSIYACNFFGVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5V0</accession>
    <name>A0A1A0H5V0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 30 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF-3 30 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HCR1</name>
        <name evidence="6" type="ORF">METBIDRAFT_79489</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709992.1">
        <property type="nucleotide sequence ID" value="XM_018859074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5V0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5V0"/>
    <dbReference type="GeneID" id="30032050"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="30285" checksum="8964DAD89A925B2C" modified="2016-10-05" version="1">MSWDDEDFDVPTTTKHAAASWEDEAEDEPVVDSWDVDEDEVARQKKAAEDKKKAELKKKQDEAKAKKNAAKSGAQTLLEIDTVDEATRREMLRKAELLADLNNAADLFGGLGVAGDSLEDELIAHPREKAGVPAKKAPAFNKDTPISDHPLFQPANKQEFEKLRKTVGAALTGLAEDSLLNYSSSLAVDLIRDLVQPLSVENLRKVISTLTVIQREKERQERQARLLKSGGTATGGAGKKKAKPAVKTNVNNPGFKKDSFDDMGGEYDDFGDDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H655</accession>
    <name>A0A1A0H655_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_45839</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710039.1">
        <property type="nucleotide sequence ID" value="XM_018857966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H655"/>
    <dbReference type="STRING" id="869754.A0A1A0H655"/>
    <dbReference type="GeneID" id="30030942"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032174">
        <property type="term" value="C:cellular bud neck septin collar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000370">
        <property type="term" value="P:positive regulation of clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031536">
        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903501">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007116">
        <property type="term" value="P:regulation of cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901901">
        <property type="term" value="P:regulation of protein localization to cell division site involved in cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000004">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="121"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="328" mass="37702" checksum="4D088B2AE0A1F80C" modified="2016-10-05" version="1">MSEHQEADIDSIVDRLLEVRGSRPGKQVTLLEHEIRFLCTKAREIFIQQPILLELEAPIKICGDIHGQYYDLLRLFEYGGFPPEANYLFLGDYVDRGKQSLETICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKMWKTFTDCFNCLPIAAIIDEKIFTMHGGLSPDLNSMEQIRRVMRPTDIPDVGLLCDLLWSDPDKDIAGWSENDRGVSFTFGPDVVSRFLQKHDMDLICRAHQVVEDGYEFFSKRQLVTLFSAPNYCGEFDNAGAMMSVDESLLCSFQILKPADKKPRYPPPSSMGNNRPVTPPRKAKRGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H694</accession>
    <name>A0A1A0H694_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein L17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_79501</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710008.1">
        <property type="nucleotide sequence ID" value="XM_018859076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H694"/>
    <dbReference type="STRING" id="869754.A0A1A0H694"/>
    <dbReference type="GeneID" id="30032052"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="222"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="248" mass="28170" checksum="8273D8963C2A5406" modified="2016-10-05" version="1">MPALKKFDANVVRHVKLMNKNLCANLIRHEYIVTGRTKAKRAQAKIESFLARAMYQNKELAEQPLALRIRQVKALGYLQPPDKAELAPKVLDELSRRFSTRTHGFTRIIKLENRLGTDNAPMSVLELVDSDFEIKFWYTAKIVARLELQGLELDGITSHNVQSLTHSRHNGTARFRDAVEECKVKFFSYDPETKSVTDAAVRAELDNKPSLRYVEQAVDFSGSKRHPVKPRGQRSSVAVPPSPFLEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H696</accession>
    <name>A0A1A0H696_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cystathionine beta-lyase</fullName>
            <ecNumber evidence="3">4.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cysteine-S-conjugate beta-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_46198</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L,L-cystathionine + H2O = L-homocysteine + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13965"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18121"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="EC" id="4.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.13" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710137.1">
        <property type="nucleotide sequence ID" value="XM_018858005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H696"/>
    <dbReference type="STRING" id="869754.A0A1A0H696"/>
    <dbReference type="GeneID" id="30030981"/>
    <dbReference type="OrthoDB" id="2545919at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000013">
        <property type="entry name" value="Cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000009">
        <property type="entry name" value="Cystathionine gamma-synthase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006238">
        <property type="entry name" value="Cys_b_lyase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01329">
        <property type="entry name" value="cysta_beta_ly_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 13 14" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="403" mass="44391" checksum="464BBC0A569166D8" modified="2016-10-05" version="1">MASKPYNIETELVFSKSTDQHNASVPPLYQSATFKQDSLLNMGEYDYTRSGNPTRTFLQNKMAAIMKASQTFAVTSGMGCLDVILRLLRPGDEVIAGDDLYGGTHRLLTYLNSKGDLKVHHHDTTDTELIKRKLTRNIKMIFLESPTNPLIKVCDVRAIAAHAHAVNPHCLVVFDNTMMTPLLMTPLDLGVDIHYESATKYLNGHHDIMAGVIAARDPALAERIYFVINSTGCGLSPFDCWLLARGLRTLAVRVERQQQNCVKIAGFLQQAGFKVRYPGLKSHPQHALHQSQCSGAGAVLSFETGLVKLLEKIVEATDIFGIAVSFGCVNSLISMPCKMSHASIDAKTREERDMPEDLIRLCIGIENIDDLVQDLTRALLKSGAVRLNANDELFNAAAAQPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6I6</accession>
    <name>A0A1A0H6I6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_217904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710174.1">
        <property type="nucleotide sequence ID" value="XM_018855106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6I6"/>
    <dbReference type="GeneID" id="30028082"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="189" mass="19762" checksum="FF7BC33CE26A6A24" modified="2016-10-05" version="1">MALGKLLGSTCVLHPAGCHLAETCFVIPCLAMYGWGHLAPRARPLRKRCMGPMGGHDRSSRGAVRDAGAAPSPSQPRGGSDVMVSFRGVGSAGPWNLKPRAVFSSSSSRSCGNEKGCRETSQGAGQTAGGLGPRPVTLPFRSTIAFSRAWSSSNWQVEFPSGRCTLCFLCILAQKSVSPSKNAGTVPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L0</accession>
    <name>A0A1A0H6L0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_28011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710076.1">
        <property type="nucleotide sequence ID" value="XM_018855347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L0"/>
    <dbReference type="GeneID" id="30028323"/>
    <dbReference type="OrthoDB" id="79687at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984:SF6">
        <property type="entry name" value="SCY1-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="657" mass="74983" checksum="37DC7EFD72A62C04" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Q6</accession>
    <name>A0A1A0H6Q6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_218011</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710168.1">
        <property type="nucleotide sequence ID" value="XM_018855107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Q6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Q6"/>
    <dbReference type="GeneID" id="30028083"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF224">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="7"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="437" mass="46521" checksum="8289128B2AEF6A93" modified="2016-10-05" version="1">MPTLQGASRASSFISLFKTIVGAGLLSMPLAFSTDGMAGGVLIVLLAAITSGYGLFLQAYVSKYVATGHATFFNVCSATYPALSVVFDLAIAIQCFGCAVSFLVLIGDLMPTVVPMIPGVPADRVRTFWIVASAAPCVPLSFLKNLDSLKSTSVMGLGAIAYLCALVVCEKALGHVPAELTGPIRLTPASLEGVFSTFPIMVFAFSGHQNMFSIINEAQDKSLASLTALIKATILCASFLFIIVGVSGYLTFGNLVGGNVVLLYPSSFWTQFGRFAIVFMVVFLFPLMIHPARISVNNIHHWVSERVSGSIGRSSQSANDETYTLLGDGESSPSFKPQRIVVPFPQNTFFIITIVLLAVAYTLAVTLESFALVIAVIGATGSTSISFILPGLFGYKLIGSEAETPSKRERTLRLFALFLTIWGFAVMAVCLYASLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6T7</accession>
    <name>A0A1A0H6T7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Ceramide very long chain fatty acid hydroxylase</fullName>
            <ecNumber evidence="18">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_12789</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Ceramide hydroxylase involved in the hydroxylation of sphingolipid-associated very long chain fatty acids. Postulated to hydroxylate the very long chain fatty acid of dihydroceramides and phytoceramides at C-2.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 19">Binds 2 Zn(2+) ions per subunit that likely form a catalytic dimetal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the sterol desaturase family. SCS7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="18"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710266.1">
        <property type="nucleotide sequence ID" value="XM_018854267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6T7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6T7"/>
    <dbReference type="GeneID" id="30027243"/>
    <dbReference type="OrthoDB" id="2204368at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080132">
        <property type="term" value="F:fatty acid 2-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102772">
        <property type="term" value="F:sphingolipid C4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000038">
        <property type="term" value="P:very long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014430">
        <property type="entry name" value="Scs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863:SF1">
        <property type="entry name" value="FATTY ACID 2-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12863">
        <property type="entry name" value="FATTY ACID HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005149">
        <property type="entry name" value="IPC-B_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="17 18" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="10 18" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 20" id="KW-0349">Heme</keyword>
    <keyword evidence="14 18 20" id="KW-0408">Iron</keyword>
    <keyword evidence="5 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 18 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11 19" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="221"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="22">
        <location>
            <begin position="5"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR005149-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA19741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="43957" checksum="26AB1134DB792123" modified="2016-10-05" version="1">MSEVLPLLSAAAVAEHNTKSNCWVTLYHRKVYDVSSFIDEHPAGGDLIIPYAGKDVTEIMANLESHEHSESAYEMLNEHMLIGYLATPEEEEELINNKKKVPVEVKLTKEAESEFDLSEFHSKLPALEKLSIQTDFSQDAKKHKFLDLNHALLPQIFRSNFSKEFYLEQVHRPRHYGKGSAPLFGNFLEPLSLTAWWVVPLVWLPLNFLLFYVGFMGQSKITALSLWAVGLFVWTFVEYCLHRFLFHLDQYLPDHKAFFTLHFLLHGIHHYLPMDKNRLVLPPTLFVLLAYPFYRLIFGIFPFYMACSGFAGGTFGYIMYDMVHYVLHHSQLPGYLQDLKTYHLEHHYKNYEMGFGVTSKFWDVIFNTEIEATSQKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Y5</accession>
    <name>A0A1A0H6Y5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710196.1">
        <property type="nucleotide sequence ID" value="XM_018857949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Y5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Y5"/>
    <dbReference type="GeneID" id="30030925"/>
    <dbReference type="OrthoDB" id="4065319at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051009">
        <property type="entry name" value="PRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36089:SF1">
        <property type="entry name" value="CHITIN SYNTHASE 3 COMPLEX PROTEIN CSI2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="336" mass="35986" checksum="5ED23EBD02B8ACC4" modified="2016-10-05" version="1">MKIAPLSPPRSLSYGGKYERKYHHGHAPFNKRLAKRSTLPSLNSATSTASTTGASTSSASTSGTSLETQESETFFTLSATISSSMATIDMTTLVSNSTTAMPTVSTTSSSSSTTSTSYTYQATVPTDNAENPYISRESLPVNSVFIIVGALLGALALGIVLSWLVVWWKSRKRAKREKEVQYYNPFHYDNSSNSSATHLLAGSLGSSFLEKSTASVNTMFSDPTSLGMGTSTPGRSYREMLNSMGRRGSMTISPVLEMMRSSASNLDLPLFHPAPDAPVSMPRTPVEISGQSNPTSRPPSRVLDDMLAAIEFSADYPETRENTEGGPLAPQDTNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H752</accession>
    <name>A0A1A0H752_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Manganese/iron superoxide dismutase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_45045</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710253.1">
        <property type="nucleotide sequence ID" value="XM_018857868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H752"/>
    <dbReference type="STRING" id="869754.A0A1A0H752"/>
    <dbReference type="GeneID" id="30030844"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="3">
        <location>
            <begin position="282"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="347" mass="37855" checksum="356174E5E5BF63F4" modified="2016-10-05" version="1">MARLAAPLRSTHKAFARLLTYKLAETPVFQHIKTSQSAFHGLYSYKAFEDLWFRQGEALTARLNAQIHDLGVDNTPADLSELIASTFNTPELAPITACAARLHNLQFCLESLRPHEAALPGGKPGAEALLRTPSIATRPDNEPAHAALREWIDDSFGSVAELRSLLLNSALAIKGDGVTWLVAQASYSASSLRGGSQADLSFSTLAVVNTYNAGVVDDAVRLGQLSKLRQQRLAREEAAARRQRERSEIAGAPRLPAEPSLDAVPAPEDPAASTVLGSVEEAEEATLYLDRKLVPLLAVDASMRAYLHDYGVFGKLQYLDSVWRCINWDVVAERAPARFKPSVVFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D8</accession>
    <name>A0A1A0H7D8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 23, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_32958</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIM23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710466.1">
        <property type="nucleotide sequence ID" value="XM_018856174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D8"/>
    <dbReference type="GeneID" id="30029150"/>
    <dbReference type="OrthoDB" id="3996489at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029427">
        <property type="entry name" value="AIM23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14877">
        <property type="entry name" value="mIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="33"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="333"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="389" mass="44370" checksum="0F6F4DE00631E46A" modified="2016-10-05" version="1">MMKRSLIVSARRFIQIPQGPYGDQNRFRAAFGNSQRTDAKQERSPDSPNTFRFSPEKSSLRGKWHHKRNDYGSEDRNKYPSRGGSWSNGRHDNQSRQAFNFSTGSERAQTALKSLILKVKSKSSNFNVNYVDPETKEFLTVSLQTLVNKLDLQKEGIRVLPPQGEGPPIIRVIPVEEMLENYSKELAALKQQELFDMGSTRAVKLANMRAQAERKKSATKILTLSWSISVSDLLNQKKNEIEKRMKKDGKLVIFVGEKSSLSAARINAEKEDVLVNELKTSNTNWNSMDEDAYLLEVKKRELIYAKLQEILQELGCKIETSGSLDTRMMIKMSSNSKSAAQSETSHQHLSPKELKKLKKLEKAKERVKAKELKLAEEEIDSLYQLKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7R9</accession>
    <name>A0A1A0H7R9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DUF396-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45505</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710669.1">
        <property type="nucleotide sequence ID" value="XM_018857922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7R9"/>
    <dbReference type="GeneID" id="30030898"/>
    <dbReference type="OrthoDB" id="28257at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097020">
        <property type="term" value="F:COPII receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007277">
        <property type="entry name" value="Svp26/Tex261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144:SF0">
        <property type="entry name" value="PROTEIN TEX261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144">
        <property type="entry name" value="TEX261 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04148">
        <property type="entry name" value="Erv26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="217" mass="25121" checksum="4B58DBE1AC77D7BC" modified="2016-10-05" version="1">MFIQILSYTGIVTGFILLVSAIALGLYYISELVEEHTEPTKRVLRKLIYIEIAMLTLLWLFDSFPFKLTMFLIFSHVVYFQNLVKFPYVQLTSPVFIFSCVLVVANHFLWFSHFHNPDIPPLSERLRPDYVPPRIPLFSEVCSFFGLCVWLIPFALFVSLSAHDNLLPHHMETATDGQGKKKKTGLAKWVVEKLRDYVYASSRFLGYELDPNYGTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U6</accession>
    <name>A0A1A0H7U6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_33056</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710621.1">
        <property type="nucleotide sequence ID" value="XM_018856207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7U6"/>
    <dbReference type="GeneID" id="30029183"/>
    <dbReference type="OrthoDB" id="431202at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903136">
        <property type="term" value="F:cuprous ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="7061" checksum="ACAF4E22CF0EC8E0" modified="2016-10-05" version="1">MSEQAQTNKSETSNKPKPCCVCLDEKKARDECLLFSGEEDGKCQEFIDKYKICMKGFGFKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8L6</accession>
    <name>A0A1A0H8L6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TUG ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_44199</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710981.1">
        <property type="nucleotide sequence ID" value="XM_018857757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8L6"/>
    <dbReference type="STRING" id="869754.A0A1A0H8L6"/>
    <dbReference type="GeneID" id="30030733"/>
    <dbReference type="OrthoDB" id="440781at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46467:SF1">
        <property type="entry name" value="TETHER CONTAINING UBX DOMAIN FOR GLUT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11470">
        <property type="entry name" value="TUG-UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TUG ubiquitin-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="502"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="513" mass="54923" checksum="227F55CA06D0550F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8M4</accession>
    <name>A0A1A0H8M4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_12284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710759.1">
        <property type="nucleotide sequence ID" value="XM_018854202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8M4"/>
    <dbReference type="GeneID" id="30027178"/>
    <dbReference type="OrthoDB" id="7697376at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="139" mass="15495" checksum="564DFE65C948E00A" modified="2016-10-05" version="1">MGFLFPPVDSQPPRSALDITELLDRLRGPALAAHQAAFSNAKHYYDRRHKHQLFSVGDEVYLDLPQLGNQLDAQNSAVLPELRSKFAGPVAIASVLSPVNCELAMPPDFRDTQFSTLVSSGSNTKFRRNVSLLLTLWFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8R7</accession>
    <name>A0A1A0H8R7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SLS1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12318</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710800.1">
        <property type="nucleotide sequence ID" value="XM_018854211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8R7"/>
    <dbReference type="STRING" id="869754.A0A1A0H8R7"/>
    <dbReference type="GeneID" id="30027187"/>
    <dbReference type="OrthoDB" id="5392646at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048401">
        <property type="entry name" value="SLS1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20778">
        <property type="entry name" value="SLS1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLS1 C-terminal" evidence="2">
        <location>
            <begin position="520"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="671"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="674"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="818" mass="87346" checksum="B92B8455F2D33439" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H904</accession>
    <name>A0A1A0H904_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_23895</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711126.1">
        <property type="nucleotide sequence ID" value="XM_018855271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H904"/>
    <dbReference type="STRING" id="869754.A0A1A0H904"/>
    <dbReference type="GeneID" id="30028247"/>
    <dbReference type="OrthoDB" id="5418899at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.240.10:FF:000085">
        <property type="entry name" value="Zinc cluster transcription factor CZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="9164" checksum="9AA76E3E0769B320" modified="2016-10-05" version="1" fragment="single">TKRSRMGCLTCRQRKKRCCETRPQCTECQRLRLNCVWPKPGTEHKNKPKEVKSQENMIDHDVYGKIKVLRGIVEYRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H951</accession>
    <name>A0A1A0H951_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_200427</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711058.1">
        <property type="nucleotide sequence ID" value="XM_018854928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H951"/>
    <dbReference type="STRING" id="869754.A0A1A0H951"/>
    <dbReference type="GeneID" id="30027904"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12455">
        <property type="entry name" value="RRM_like_Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005120">
        <property type="entry name" value="UPF3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03467">
        <property type="entry name" value="Smg4_UPF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF3" evidence="2">
        <location>
            <begin position="45"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="321" mass="36349" checksum="4E9B01D7EAD8163F" modified="2016-10-05" version="1">MLFPYLKKTAMEPSKKVAQNIKSKRVRRKQRQKKTGLLIEYGDPDTKVAVRLLPPTLEEMDFREQAEKISLTFRSGFESFTYKKGKKASKPFEEPCFSVAFVKFKTPALAENFKREISHVLFKETTTEDNIKCNVMRPIFGELNTSQTTSSEIPIETEVFKLFASLRGQKEGHVDIHEVLATCRSKTRGKKTKLKLSTPTSLEHLNQSSFSPGIVKGSKRKDHKHADKPNDPKLKSKRITSHTSNFSEHESKPKNKQKQRKLPESIPHKSTDAQPSGPLLSTSGVLNKKTKKKKKPLGSHTPLAKNIKNLCDGKPILEKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H969</accession>
    <name>A0A1A0H969_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RCC1/BLIP-II protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32539</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711078.1">
        <property type="nucleotide sequence ID" value="XM_018856074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H969"/>
    <dbReference type="STRING" id="869754.A0A1A0H969"/>
    <dbReference type="GeneID" id="30029050"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006997">
        <property type="term" value="P:nucleus organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="51"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="5">
        <location>
            <begin position="53"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="111"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="187"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="240"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="293"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="352"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="419"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="125"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="490" mass="53649" checksum="CBB68656B9E9F730" modified="2016-10-05" version="1">MVALNRTLKRSASPVQDGRHKRLRSTSSNVLQSYSRLPTIIDRPPPKSQSLDIFVWGTGSMCELGLGPQAKTKEVKRPRLNPFLTSEKLGADGIVDFSVGGMHVLALDSNNVLWSWGTNDSKVLGRDTTGKENLKSMDDAEDSDDDDGDLNSAESTPGKVENLPKTNIKIVQVAATDNLSAVLYENGEVYTWGTFRSNEGLLGYSKEVNTQEYPAKVNNLENIVQLATGKDHVLALDAKGIVYAWGNGQQSQLGRKILERHKFTALQPHTFGLYDIKYIASGDFHCFAIDSNGKVFAWGLNQYGQCGITNKQGELEDGSVIQKPTIIPALEDKNIVEIVGGEHHSLALTGEGELFTWGRYDMKEVGIAKENLPDYTYRDQHGLPRSVPIPTLLRIADGTLKFKAIAAGSHHSFAVTRDGIVYAWGFGDTYGPGLGLLDGDVEIPTRIYNTATKDMDIQIIGAGGQFSVSGGRKLSDTHAAERNEKYEIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H972</accession>
    <name>A0A1A0H972_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ras-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_43531</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Lipid-anchor</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the small GTPase superfamily. Rheb family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711083.1">
        <property type="nucleotide sequence ID" value="XM_018857671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H972"/>
    <dbReference type="STRING" id="869754.A0A1A0H972"/>
    <dbReference type="GeneID" id="30030647"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04137">
        <property type="entry name" value="RheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000273">
        <property type="entry name" value="GTP-binding protein Rheb homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20931" checksum="B53DCACC2B8CB821" modified="2016-10-05" version="1">MVKARKIAMVGSRSVGKLSMTVRFVEDHFVESYYPTIENQFSKTITYKNQEYAIEILDTAGQDEFSIMTEKHLIGIHGYLLVYSVTSRQSFELIEIIRDKILNSIGMDSVPVVLLGNKCDLNFQRQVEIHEGEALARKFKCKFLETSVRDNINIEQAFVSLVEEIELIQNPETPKEEKCVMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9L7</accession>
    <name>A0A1A0H9L7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32552</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711095.1">
        <property type="nucleotide sequence ID" value="XM_018856080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9L7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9L7"/>
    <dbReference type="GeneID" id="30029056"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="899" mass="104273" checksum="F92DBB66B741EA22" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9P3</accession>
    <name>A0A1A0H9P3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome complex subunit SEM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_32577</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DSS1/SEM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711120.1">
        <property type="nucleotide sequence ID" value="XM_018856089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9P3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9P3"/>
    <dbReference type="GeneID" id="30029065"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007834">
        <property type="entry name" value="DSS1_SEM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771">
        <property type="entry name" value="26 PROTEASOME COMPLEX SUBUNIT DSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771:SF0">
        <property type="entry name" value="26S PROTEASOME COMPLEX SUBUNIT SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05160">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01385">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="68" mass="8240" checksum="5F9562ADF2CD1A57" modified="2016-10-05" version="1">MSEQEQEKIIKTLEEEDEFEDFPEDAQWTSEPNSQLNSANLWEEDWDDDDDNDEFTEKLREELKKAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9T0</accession>
    <name>A0A1A0H9T0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_205698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711293.1">
        <property type="nucleotide sequence ID" value="XM_018854980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9T0"/>
    <dbReference type="GeneID" id="30027956"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="82" mass="9875" checksum="91C816695A740279" modified="2016-10-05" version="1">MSTNCQRTLVDFSKQTRRGSAFCFAFLFFLFCFYVFLFCFYVFLFCFYVILFCFYVFLFCFYLFPHFGSISARRGSEYALPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9U6</accession>
    <name>A0A1A0H9U6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_205697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711292.1">
        <property type="nucleotide sequence ID" value="XM_018854979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9U6"/>
    <dbReference type="GeneID" id="30027955"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291886" evidence="1">
        <location>
            <begin position="17"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="210" mass="24239" checksum="E2B12C6EFDF7D4B4" modified="2016-10-05" version="1">MKAWCWACSWGLRGVCFLCDWRIHFRSPTPAGQPGCYQTKASLNTLLFHSDGVGMYLELQCNWDHAWFKPSFGKVQRKMPRIRKLSWRPRFSRKNKRGCRKGKNGGTRARPYPEDVARLAWPIHLGPRPAHSALIATWLPEMRASATWYFSIWHIATMARGPGCVSGYIPRYGSGISSLLEKQKIHWPPQDIRVHWKRTIFGADEKAVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA06</accession>
    <name>A0A1A0HA06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_21515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711474.1">
        <property type="nucleotide sequence ID" value="XM_018855085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA06"/>
    <dbReference type="STRING" id="869754.A0A1A0HA06"/>
    <dbReference type="GeneID" id="30028061"/>
    <dbReference type="OrthoDB" id="1938621at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43011:SF1">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="3">
        <location>
            <begin position="50"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="19307" checksum="6054A767D06F6E15" modified="2016-10-05" version="1" fragment="single">PKSFKSLSFALPKETPQSVTDSASEDAPKTTEKLTDFDMTSLIRGTTDLKIGITDRAKAKLVEIAKDDRLADLALKVKVESGGCHGFQYDFELSSLKEELEKDDELVVFQRRDSNMATVVFDESSLEILQDSKLDYTKELIGSSFKMVDSPYTSTSCGCGASFDFDFEKLENAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA18</accession>
    <name>A0A1A0HA18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711240.1">
        <property type="nucleotide sequence ID" value="XM_018854254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA18"/>
    <dbReference type="STRING" id="869754.A0A1A0HA18"/>
    <dbReference type="GeneID" id="30027230"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="303" mass="32892" checksum="094C2E949D7E69FC" modified="2016-10-05" version="1">MGILVWSLDVLVLDVLVLDVLVLGVLVLDVLVLDVLVLEMLVLEMLVLDVLVLGALVLDVLVLDVLVLGALVLGAFVLEMLVLVTMVLGYHDTGSRWCWVNIILSYYVPGCYDTGLLWYWVTIVPDFGIGCFGIGCFGIGCFGVGCFGIGDVGIGDVGIGDVGIGDVGMGDVGMGDVGIGDVGIGFIRYCVIMFLGDFDLGLLKYWVIIAPGCYGTGLLWYWGTIIPRCYGTDFIGCFGIGYFDIGCFGIGYFGMEDFGIGSFDIGDLDIRYFDIGDLNIRYFDIGDLNIRYFELGLFDFELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA26</accession>
    <name>A0A1A0HA26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_11469</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711365.1">
        <property type="nucleotide sequence ID" value="XM_018853999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA26"/>
    <dbReference type="GeneID" id="30026975"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034756">
        <property type="term" value="P:regulation of iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04591">
        <property type="entry name" value="CBS_pair_voltage-gated_CLC_euk_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000011">
        <property type="entry name" value="Chloride channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF9">
        <property type="entry name" value="ANION_PROTON EXCHANGE TRANSPORTER GEF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9" id="KW-0868">Chloride</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="443"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="576"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="659"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="755" mass="83525" checksum="63EB7F9FE13464B8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAN7</accession>
    <name>A0A1A0HAN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_173163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711575.1">
        <property type="nucleotide sequence ID" value="XM_018854704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAN7"/>
    <dbReference type="GeneID" id="30027680"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="132" mass="14817" checksum="B955FF9513656C87" modified="2016-10-05" version="1">MRAAPRNPMPSPLFPDFGGRGLTGPNPGYDSFEGGFRCGPALWPFCPLYCRVRTLSFFRWFFDDRFFVGIFLIARLFFVCISYQIFAYSFSLSFLQVLFVLACAFGLSFGYSNTSGRARACSHDLCPGPALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB59</accession>
    <name>A0A1A0HB59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711735.1">
        <property type="nucleotide sequence ID" value="XM_018857492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB59"/>
    <dbReference type="STRING" id="869754.A0A1A0HB59"/>
    <dbReference type="GeneID" id="30030468"/>
    <dbReference type="OrthoDB" id="2128042at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031606">
        <property type="entry name" value="Kch1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424:SF1">
        <property type="entry name" value="LOW AFFINITY K(+) TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16944">
        <property type="entry name" value="KCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="294" mass="34600" checksum="7268AEC0CE8E2C39" modified="2016-10-05" version="1" fragment="single">MFDSEKKAEGLDTSTFDIVKIDSFHNTRFWTVLSFVWVWIVLILKIAILGGDTYTCVSILVFNRWGTLDYDVYEYKVAKWIFTGCIFFRFLLLAYQIAWGIHVYRTRNIALAYLNHYARLMYAIRSYNYQCLFHEIDQEGFFEWACFHVYTELDNALEVLVADLPRQVINFMTLRYYATDGDLNNAILTNIKSIATTNLRLSIILSFQLCTMVLFLFFFFKFLLAIVLYIPIKVKVAHRGFRLLKAFCYKSVNENVRFLVKRNHKPRTQILNEGLMDIKEIQANLLLASNSTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBE6</accession>
    <name>A0A1A0HBE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acid protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_11848</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711820.1">
        <property type="nucleotide sequence ID" value="XM_018854113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBE6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBE6"/>
    <dbReference type="GeneID" id="30027089"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291729" evidence="6">
        <location>
            <begin position="17"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="75"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="41468" checksum="20EB961A47E54BDE" modified="2016-10-05" version="1">MTRLLSLLCLSASVFGYSISEIEKPEAPAPLKFDFSIEKKIGDLSARDFWTQNGKRKNKRDEVAVLIHNVQDIYYMLNVYLGSNKQSNYVVIDTGSSDLWVPSSGYDAKTSTTSEDTDIPFGIMYIDGSVAQGEYYKDSFLFGTSESILDSFQFALSNASGPGILGIGNKAQEVATLKYDNFPWALQNAGITPKASYSLYLNPNSDSGVVIFGGIDTEKYDGCLSKYPVYDSGNDLSINVESVYMGEKEIEINSPYSIDSGTSLGLVGQELMDELDIIFDATVVEMDGTSYRVISCTQPDDQYVVFNFGANEILISYADLVLKNDDDSCILGLAYFEGYQVLGDVFMKHAYVYFDLTDQTIALAQAVSSEKSNIITA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBE9</accession>
    <name>A0A1A0HBE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78241</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711712.1">
        <property type="nucleotide sequence ID" value="XM_018858849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBE9"/>
    <dbReference type="SMR" id="A0A1A0HBE9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBE9"/>
    <dbReference type="GeneID" id="30031825"/>
    <dbReference type="OrthoDB" id="21225at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000146">
        <property type="entry name" value="Putative two-component response regulator SSK1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="357"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="217"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="266"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="537" mass="60480" checksum="149837BDC4D162C2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBI8</accession>
    <name>A0A1A0HBI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_42126</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PI3/PI4-kinase family. TRA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711752.1">
        <property type="nucleotide sequence ID" value="XM_018857500.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HBI8"/>
    <dbReference type="GeneID" id="30030476"/>
    <dbReference type="OrthoDB" id="5570127at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05163">
        <property type="entry name" value="PIKK_TRRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046807">
        <property type="entry name" value="Tra1_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046805">
        <property type="entry name" value="Tra1_ring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20175">
        <property type="entry name" value="Tra1_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20206">
        <property type="entry name" value="Tra1_ring"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAT" evidence="4">
        <location>
            <begin position="2611"/>
            <end position="3166"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="3">
        <location>
            <begin position="3436"/>
            <end position="3774"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="5">
        <location>
            <begin position="3770"/>
            <end position="3803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="496"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="3176"/>
            <end position="3252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="170"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3180"/>
            <end position="3190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3193"/>
            <end position="3207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="3216"/>
            <end position="3230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="3231"/>
            <end position="3252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="3803" mass="434293" checksum="C907D1C42C186A6B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBP2</accession>
    <name>A0A1A0HBP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like modifier HUB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_40985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711941.1">
        <property type="nucleotide sequence ID" value="XM_018857338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBP2"/>
    <dbReference type="STRING" id="869754.A0A1A0HBP2"/>
    <dbReference type="GeneID" id="30030314"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000753">
        <property type="term" value="P:cell morphogenesis involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01791">
        <property type="entry name" value="Ubl_UBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000052">
        <property type="entry name" value="Ubiquitin-like protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039732">
        <property type="entry name" value="Hub1/Ubl5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13042">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="73" mass="8357" checksum="0C1800ADD584479F" modified="2016-10-05" version="1">MIEVLANDRLGKKIKVKCLASDTIGDLKKIISLQVGTSWEKLVLKKGHQTYKDHITLDDYEVHSGFNFELYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC61</accession>
    <name>A0A1A0HC61_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_27101</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712106.1">
        <property type="nucleotide sequence ID" value="XM_018855331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC61"/>
    <dbReference type="STRING" id="869754.A0A1A0HC61"/>
    <dbReference type="GeneID" id="30028307"/>
    <dbReference type="OrthoDB" id="448399at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071502">
        <property type="term" value="P:cellular response to temperature stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031445">
        <property type="term" value="P:regulation of heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.10">
        <property type="entry name" value="Zinc finger, RanBP2-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111:SF40">
        <property type="entry name" value="RNA-BINDING PROTEIN INVOLVED IN HETEROCHROMATIN ASSEMBLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23111">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00641">
        <property type="entry name" value="Zn_ribbon_RanBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00547">
        <property type="entry name" value="ZnF_RBZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="228"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="7">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="RanBP2-type" evidence="7">
        <location>
            <begin position="418"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="449" mass="49137" checksum="D5B6A351702903F6" modified="2016-10-05" version="1" fragment="single">MADAYVVVQLAATFDEPAVYVPRDAGELMALSWCTVDARTLETLARQSILVRPLGTPVTPACALRYSLLWDDVKDGATFKAAMAEFDRRLTAQVAGKDFLFVSVDVPGLRVLLPREARDKAVVLPGYLQHPRVFDLCNEYSKWQAVHPEALSYPSSSLANVITALGVDVGPAWADESPDAYTVAVDVHAKILAQLLQKSVPVESHPAVLTQPYDAALDAKIFLAERSKILYLCNLPADTTHSELEAWFAQAGGRPIAFWTLKNLDSDARLGLQAAGAKAKAVCGFAVFSKHEEAAEALYMNGRVLMDRVVEVQASSTRVLDRASSLLTPFPPLKNRPRPGDWTFLSCGFSNFQRRIACFRCSFPATQSYAGSGGYQDQYQGNGLKNGNPYNSGYSHNFNHSHGGTSQRSHYSNQVPFRAGDWKCANESCQYHNFAKNLCCLKCGASKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC71</accession>
    <name>A0A1A0HC71_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32194</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712239.1">
        <property type="nucleotide sequence ID" value="XM_018856000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC71"/>
    <dbReference type="STRING" id="869754.A0A1A0HC71"/>
    <dbReference type="GeneID" id="30028976"/>
    <dbReference type="OrthoDB" id="14252at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015227">
        <property type="term" value="F:O-acyl-L-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902603">
        <property type="term" value="P:carnitine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF4">
        <property type="entry name" value="CONGESTED-LIKE TRACHEA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="103"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="195"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="282" mass="29434" checksum="EB9046B40493507B" modified="2016-10-05" version="1">MDETDSILVDNIKSFASGGFGGVCAVVTGHPFDLVKVRLQTGVYKTTSECLKGTLAKDGLLGFYRGVMPPLLGVTPMFAVSFWGYDMGKKLVSGYSGTKVEDFTVGQISTAGFISAIPTTAVAAPFERVKVMMQVQSGAKSSMGSVIAEMYRTGGLRSIFKGSAATLARDGPGSALYFATYEYVKAALSSPDKDLSLGAISVAGGCAGMSMWLGVFPIDTIKSTQQSSNVNISIATATKNIYAKAGIKGFFPGVGPALARSFPANAATFVGVELAKNFLEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI0</accession>
    <name>A0A1A0HCI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Biogenesis of lysosome-related organelles complex 1 subunit CNL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CNO-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_27524</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the biogenesis of lysosome-related organelles complex-1 (BLOC-1), a complex that is involved in endosomal cargo sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BLOC1S4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712133.1">
        <property type="nucleotide sequence ID" value="XM_018855337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI0"/>
    <dbReference type="GeneID" id="30028313"/>
    <dbReference type="OrthoDB" id="5424991at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031083">
        <property type="term" value="C:BLOC-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034455">
        <property type="entry name" value="CNL1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39145">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT CNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39145:SF1">
        <property type="entry name" value="BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT CNL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="11826" checksum="DEEE657D00EFCC40" modified="2016-10-05" version="1" fragment="single">DDDPLLLKDLAVDFDYLMFKIKDHILGLAELTNTSVASKKQLIENDYFAQQLQLDAQLADADSTLKECAELEMLFLKLSQLRQFVDGFKTRLASLEHEYAKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCP5</accession>
    <name>A0A1A0HCP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_32158</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712193.1">
        <property type="nucleotide sequence ID" value="XM_018855992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCP5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCP5"/>
    <dbReference type="GeneID" id="30028968"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="486"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="603" mass="68120" checksum="A260508081449F37" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCR0</accession>
    <name>A0A1A0HCR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_10720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712287.1">
        <property type="nucleotide sequence ID" value="XM_018853835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCR0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCR0"/>
    <dbReference type="GeneID" id="30026811"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="144"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="146"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="217" mass="24347" checksum="4DBB9F15A62D196A" modified="2016-10-05" version="1">MNKTTTSLPPISDIFRGPNITDPYYPQQGYSMYGGMSVVPHTYPAPQYQLATPEQVAPRYYNAPYTIPAYGFAGKTGYGDARDAWRGGVDVPHQLPSPTLYQSPAQLGHRGMSMSVGLSPLDYADIQTLSREMSPKSQHMAASGSKMRTRNNLPKEVTSVLLKWLNEHLNHPYPNSFEKAQLIIATGLNQQQLSNWFINARRRKIKSLREKRDSQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS0</accession>
    <name>A0A1A0HCS0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidyl-tRNA hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_10727</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712297.1">
        <property type="nucleotide sequence ID" value="XM_018853836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCS0"/>
    <dbReference type="GeneID" id="30026812"/>
    <dbReference type="OrthoDB" id="1711136at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1470">
        <property type="entry name" value="Peptidyl-tRNA hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001328">
        <property type="entry name" value="Pept_tRNA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036416">
        <property type="entry name" value="Pept_tRNA_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17224:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01195">
        <property type="entry name" value="Pept_tRNA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53178">
        <property type="entry name" value="Peptidyl-tRNA hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="21817" checksum="592639A8EC4EFF0B" modified="2016-10-05" version="1">MSSIVFFSVGNPGKMARHSVGHYILKNLMDALGAKQLVKKGVYSISTMDSIYFVKSNAYMNESGKLLRKFLDSEKLGQCVVIVIYDDFETPMPKVKLQQFKKNESHGGIKSTARELQAQAVNGYKLGIGIGPKPAQASRELMASWVLSDFKEEEKLALATSMDSVYRYTDAIISSEGEVDDCNKLNARVTKAMKSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD37</accession>
    <name>A0A1A0HD37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77373</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712303.1">
        <property type="nucleotide sequence ID" value="XM_018858683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD37"/>
    <dbReference type="STRING" id="869754.A0A1A0HD37"/>
    <dbReference type="GeneID" id="30031659"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="12">
        <location>
            <begin position="521"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="286"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="683"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="860"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1017"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1238" mass="140665" checksum="3F7B9E7C87622384" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK3</accession>
    <name>A0A1A0HDK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial pyruvate carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_77559</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the uptake of pyruvate into mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712551.1">
        <property type="nucleotide sequence ID" value="XM_018858722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDK3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDK3"/>
    <dbReference type="GeneID" id="30031698"/>
    <dbReference type="OrthoDB" id="869189at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006850">
        <property type="term" value="P:pyruvate import into mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005336">
        <property type="entry name" value="MPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14154">
        <property type="entry name" value="UPF0041 BRAIN PROTEIN 44-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03650">
        <property type="entry name" value="MPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="118" mass="13280" checksum="0C3AFC90912B0F38" modified="2016-10-05" version="1">MAASAQQVSKFSRYLQSETGPKTVHFWAPVFKWSLVIAGLNDIQRPVEKISGTQQLALFFTGAIWTRWAGFAITPKNYLLASVNFFLGGVAGYQLIRVVNYRRAEGDSPAQVFKYLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDU1</accession>
    <name>A0A1A0HDU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31028</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712558.1">
        <property type="nucleotide sequence ID" value="XM_018855756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDU1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDU1"/>
    <dbReference type="GeneID" id="30028732"/>
    <dbReference type="OrthoDB" id="3631276at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021988">
        <property type="entry name" value="BMT1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12141">
        <property type="entry name" value="BMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="588" mass="66814" checksum="B09862CDA74A125D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDU8</accession>
    <name>A0A1A0HDU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">37S ribosomal protein YMR-31, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_40638</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-ketoglutarate dehydrogenase component 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712651.1">
        <property type="nucleotide sequence ID" value="XM_018857297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDU8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDU8"/>
    <dbReference type="GeneID" id="30030273"/>
    <dbReference type="OrthoDB" id="2116030at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020373">
        <property type="entry name" value="Kgd4/YMR-31"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10937">
        <property type="entry name" value="Kgd4-YMR31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10315" checksum="C0EE6B2A8B1CE77F" modified="2016-10-05" version="1">MRQSASLLKHVPLIKFVGGPHQAATVAAKAHPFSPSGKMPTLQKTQSSQPAWKPIELQEGEVLLRNQLSKRFRYMVPSTEESEQIESGGAGLVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDY7</accession>
    <name>A0A1A0HDY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_40431</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018857273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDY7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDY7"/>
    <dbReference type="GeneID" id="30030249"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000260">
        <property type="entry name" value="Spalt-like transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF142">
        <property type="entry name" value="ZINC FINGER PROTEIN 384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20298" checksum="DB68F897AA9CC542" modified="2016-10-05" version="1">MKSHTLGKTHECSVCHKSLSSKNALAHHSRRHTGGHAFECAICKKGVAKKSLLSYHMRSHTGEKPFACGLCNRRFSLRGNLTAHIRMHTREKTYQCPVCQERLPSRNALRLHKQSHLTAASHECSICRKTLSSNAHLLDHLRTHSGDRPFKCPVCSKAFPSSGNLARHTKTHKRQDGHMGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE17</accession>
    <name>A0A1A0HE17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_11192</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712840.1">
        <property type="nucleotide sequence ID" value="XM_018853928.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HE17"/>
    <dbReference type="GeneID" id="30026904"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291804" description="Flo11 domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="587"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="704"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="788"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="859"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="921"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1058"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="190"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1069"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1099"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1202"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1240"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1260"/>
            <end position="1279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1420" mass="142561" checksum="0A56F2A08D9906CD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE31</accession>
    <name>A0A1A0HE31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712855.1">
        <property type="nucleotide sequence ID" value="XM_018853932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE31"/>
    <dbReference type="STRING" id="869754.A0A1A0HE31"/>
    <dbReference type="GeneID" id="30026908"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF70">
        <property type="entry name" value="PROTEIN OCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="399" mass="44446" checksum="08860898F74D5C01" modified="2016-10-05" version="1">MLIPPDNFGMVEPGVYRCSKLEADHLPFLETLRLKLLVLLDVAKPPRMLKPFLQENHVDLYDLGGLKILNHQNTGPVLHDRDRLVAEVDPRVENVDKKLDTHVDIRVDNKVDPKVDTKVGNKLESKLESKLESKLESKLESKLESKLESKLESKLDNKLDNKPENKVATKADPKADPKVDTNLELPNTLALPSTPPAGRPRQPAADPDVVHVPPALPQKHDLWMIIEKNVIAAACAILLDRTKHAVLVVDSTLALVGILRKLQKWNFNSIVNEYRIYTGNASKISYGVEVFLELLQLELVPDEIDRLARRHEPTSPPRPGPAAADPGPALRRPLAPCSPAHNTTRSSWATPHPNSDSLFGSFPVSLFYQCWLQACQLLRWTPAAHITILVTRRAGLAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE33</accession>
    <name>A0A1A0HE33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4536 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_38696</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712659.1">
        <property type="nucleotide sequence ID" value="XM_018857032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE33"/>
    <dbReference type="GeneID" id="30030008"/>
    <dbReference type="OrthoDB" id="4083608at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10084" checksum="B9407B998EBCFF73" modified="2016-10-05" version="1">MSNILNVLSPPPGRDLSDEECIGCTAVQLAVCFLGGGYFLSLLPFKDKSGLVDLRKHPVWFQRGVRGTGIGLLALACYRLGEVGKIYYNRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE79</accession>
    <name>A0A1A0HE79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712791.1">
        <property type="nucleotide sequence ID" value="XM_018853923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE79"/>
    <dbReference type="STRING" id="869754.A0A1A0HE79"/>
    <dbReference type="GeneID" id="30026899"/>
    <dbReference type="OrthoDB" id="342454at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46620:SF1">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN SPF31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="31"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="226" mass="26155" checksum="05D6E821C447534B" modified="2016-10-05" version="1">MADLDALFQTQESEVARDREIARVLRCPAKDHFAILQINPLTEHASLAATLRKTYRKKSLQIHPDKTKNSDAPRAFDLLKKANSVLSAEPPSTSSGANGDHEDQHSLLEENARYAQKEYLILIYKQVADGLGAFHVDDFHHATNRAIRDKVLLVLEQHEKDRAVETGYKQRQEIKKQTEFQTAAKERELKKSWETRWEQDRDTRVKLWRTFSTKVEKPKKKKKLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE94</accession>
    <name>A0A1A0HE94_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zn-dependent exopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_31230</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712806.1">
        <property type="nucleotide sequence ID" value="XM_018855816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE94"/>
    <dbReference type="STRING" id="869754.A0A1A0HE94"/>
    <dbReference type="GeneID" id="30028792"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF72">
        <property type="entry name" value="ZINC METALLOPROTEASE TRE2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M28" evidence="4">
        <location>
            <begin position="430"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="3">
        <location>
            <begin position="698"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="826" mass="92029" checksum="2A7A8B32447C70B4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEC0</accession>
    <name>A0A1A0HEC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YMC020W-like alpha/beta hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_38897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712955.1">
        <property type="nucleotide sequence ID" value="XM_018857065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEC0"/>
    <dbReference type="GeneID" id="30030041"/>
    <dbReference type="OrthoDB" id="5598028at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058934">
        <property type="entry name" value="YMC020W-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058933">
        <property type="entry name" value="YMC020W-like_ab_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349:SF1">
        <property type="entry name" value="AER328WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47349">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26147">
        <property type="entry name" value="AB_HYDROLASE_YMC0-YMC35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YMC020W-like alpha/beta hydrolase" evidence="2">
        <location>
            <begin position="245"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="652" mass="72538" checksum="8095BB2833BD8CEC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEF5</accession>
    <name>A0A1A0HEF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWR1-complex protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_17640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712990.1">
        <property type="nucleotide sequence ID" value="XM_018854731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEF5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEF5"/>
    <dbReference type="GeneID" id="30027707"/>
    <dbReference type="OrthoDB" id="445677at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011421">
        <property type="entry name" value="BCNT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027124">
        <property type="entry name" value="Swc5/CFDP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48407:SF1">
        <property type="entry name" value="CRANIOFACIAL DEVELOPMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07572">
        <property type="entry name" value="BCNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51279">
        <property type="entry name" value="BCNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BCNT-C" evidence="5">
        <location>
            <begin position="201"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="281" mass="32263" checksum="0E9C43A6A2337AAB" modified="2016-10-05" version="1" fragment="single">DENDEDYDENNDDDYDPLKKADVQDDDSEMSEDEAQPDYSAISTDVSQVRTRGQRYQEKAEQKNGKTHQDGLLEDSLKVDVDEIFQSLQTLSADDWARMVRHDDATPEPRGKDEKPAAALAENTHPEAEEKIRIFTTYTFAGKLVRESKFVDANSAEAKAYLNSTNSLALQEQSAGPRKSYVPVLRKVAGSDEEKQLRIKLKRPSLIDKFLMGDKQNKLTTLEKSRLDWASFVDKKKLKDDLAIHNKGGYLEKQDFLGRVEAKRDTQYQQARELDRQRQWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEG9</accession>
    <name>A0A1A0HEG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">JmjC-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_38904</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712793.1">
        <property type="nucleotide sequence ID" value="XM_018857067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEG9"/>
    <dbReference type="STRING" id="869754.A0A1A0HEG9"/>
    <dbReference type="GeneID" id="30030043"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034647">
        <property type="term" value="F:histone H3K4me/H3K4me2/H3K4me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15543">
        <property type="entry name" value="PHD_RSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="10">
        <location>
            <begin position="11"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="185"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="11">
        <location>
            <begin position="343"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="657"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="715"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="717"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="832" mass="95510" checksum="912E8E35CADAA0CB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEH8</accession>
    <name>A0A1A0HEH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Putative Na+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_31228</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712804.1">
        <property type="nucleotide sequence ID" value="XM_018855814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEH8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEH8"/>
    <dbReference type="GeneID" id="30028790"/>
    <dbReference type="OrthoDB" id="5327978at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1530.20:FF:000015">
        <property type="entry name" value="Na(+)/H(+) antiporter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013928">
        <property type="entry name" value="Cation/H_antiporter_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08619">
        <property type="entry name" value="Nha1_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="413"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="13">
        <location>
            <begin position="25"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="14">
        <location>
            <begin position="462"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="domain" description="Alkali metal cation/H+ antiporter Nha1 C-terminal" evidence="14">
        <location>
            <begin position="779"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="482"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="618"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="773"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="580"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="776"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="789"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="828" mass="91869" checksum="2E36B62D94D5C501" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEK3</accession>
    <name>A0A1A0HEK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Not3-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_40397</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713040.1">
        <property type="nucleotide sequence ID" value="XM_018857268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEK3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEK3"/>
    <dbReference type="GeneID" id="30030244"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012270">
        <property type="entry name" value="CCR4-NOT_su3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007207">
        <property type="entry name" value="Not_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04065">
        <property type="entry name" value="Not3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005290">
        <property type="entry name" value="NOT_su_3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-Not complex component Not N-terminal" evidence="12">
        <location>
            <begin position="3"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="13">
        <location>
            <begin position="359"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="245"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="261"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="499" mass="56563" checksum="D315D40674B8FEC0" modified="2016-10-05" version="1">MSSRKLQQEFDKLQKKVAEGLQQFEEIYEKIGVTENPSLKDKLEGDLRKEIKKLQRSRDQVKQWLGDSSNKLDKQVLLDTRSRIENAMERFKEVEKVSKMKQFSNEGLELQTKLGARGLDEARKNDAAKYVVDVLDELGRQNELLQAEMAQHSHKKRLAAAQAAHADAAAKFERNSSHVLRLELVLRHLENHQLEPEKIDEIRDDLDYYVENNQLSDFVEYDDFYEVLQLSEPPAEAPHVAPEVPAELPATPSPRKAHAAAADKRPADHKRAPELLPRKKAMTHAAAAAASAKAPAPPAAAAADSPVATPHASPLPVHGKPPRTAAPFWDNSAGIAALAHNRLHQPAPFPAISRQLEASLLNCPDSFDLERPRQYSPTNVHPSLVDYPQEPMYELNSATLMRKFDTDTLFFCFYYNEAQDALAKWHAARELLRRGWVFNLATKQWFSKEDRPKSRSVLGALHNGDRAAAASFRYFDYENSWLVRHKDNFAFDAELQWTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEW9</accession>
    <name>A0A1A0HEW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_39444</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713016.1">
        <property type="nucleotide sequence ID" value="XM_018857143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEW9"/>
    <dbReference type="GeneID" id="30030119"/>
    <dbReference type="OrthoDB" id="4081497at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="15620" checksum="327F87C8149ECCE8" modified="2016-10-05" version="1">MSQRPPPGSHRDLLTGAKQQQAAHLQTAAAASAQLIRAVESIVRVNSATAPLARTVLLDKVATIHELDAEIDRQLAQDIAENYEALLAAKQKLARHVTRTQRALAMLRHSAGSCVDVLQLKAELIDQELRVLETALSYINNQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF18</accession>
    <name>A0A1A0HF18_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein 8 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_29321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713205.1">
        <property type="nucleotide sequence ID" value="XM_018855380.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HF18"/>
    <dbReference type="GeneID" id="30028356"/>
    <dbReference type="OrthoDB" id="289913at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025941">
        <property type="entry name" value="Vps8_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 8 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23410">
        <property type="entry name" value="Beta-prop_VPS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12816">
        <property type="entry name" value="TPR_Vps8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23413">
        <property type="entry name" value="zf_RING_Vps8_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 8 central" evidence="2">
        <location>
            <begin position="659"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1366" mass="153160" checksum="0E1DF92136ABA9F6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF43</accession>
    <name>A0A1A0HF43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Superoxide dismutase copper/zinc binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713117.1">
        <property type="nucleotide sequence ID" value="XM_018855863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF43"/>
    <dbReference type="STRING" id="869754.A0A1A0HF43"/>
    <dbReference type="GeneID" id="30028839"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11423" checksum="01AB205B4085E271" modified="2016-10-05" version="1">MPVPADGNCEGTLGHFNPYSGIQNAGSLAEFEVGDLSGKHGVINGSSLRESYSDQFISLNPGNRAFVGDRSIVVHYANMTRLACANIVREDLVAPVEKRQLRVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF76</accession>
    <name>A0A1A0HF76_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_92656</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713270.1">
        <property type="nucleotide sequence ID" value="XM_018859346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF76"/>
    <dbReference type="GeneID" id="30032321"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="193" mass="21884" checksum="27CABDEDCD185A3B" modified="2016-10-05" version="1">MTVYINPINDMFLVEGKKQEITLGEKKRLLLRNSFSLPRERRLRGGYRVTCSNCGLSTAYSQSIACPCWESMDSWVIGFLTTHQDGKKPIFRIWHDQLSRDWPSKWSNEKATSSCSSRLGPGPSLKTGVLLITLTGGFLQKWTCIPRSSTSLKCIGCESFSPHIRVVFFVGPVHLDLFSKTHCCRYPARLLHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFC7</accession>
    <name>A0A1A0HFC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome c oxidase assembly protein COX19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_80394</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for the assembly of mitochondrial cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the COX19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713330.1">
        <property type="nucleotide sequence ID" value="XM_018859199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFC7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFC7"/>
    <dbReference type="GeneID" id="30032175"/>
    <dbReference type="OrthoDB" id="268594at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051383">
        <property type="entry name" value="COX19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21107:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="11869" checksum="B6F91D3F2BA63975" modified="2016-10-05" version="1">MASGAPGGNFRAWTPTPPERGSFPLDHLGECKQQMMDYLKCMKFTENKNAPNCRALAKKYLGCRMENELMEDSDWVLLGLVNLPGDTVTSHPMSQKSETKETEDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFV0</accession>
    <name>A0A1A0HFV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_62367</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SLC12A transporter family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713253.1">
        <property type="nucleotide sequence ID" value="XM_018858324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFV0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFV0"/>
    <dbReference type="GeneID" id="30031300"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000013">
        <property type="entry name" value="Solute carrier family 12 member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF72">
        <property type="entry name" value="GH08340P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="445"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="34"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="877"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="955"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="955"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="993"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1021"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1040"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1059"/>
            <end position="1068"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1269" mass="140116" checksum="352EF18D63AFE9AB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY3</accession>
    <name>A0A1A0HFY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">4-hydroxybenzoate polyprenyltransferase, mitochondrial</fullName>
            <shortName evidence="10">4-HB polyprenyltransferase</shortName>
            <ecNumber evidence="10">2.5.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">4-hydroxybenzoate hexaprenyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Para-hydroxybenzoate--polyprenyltransferase</fullName>
            <shortName evidence="10">PHB:PPT</shortName>
            <shortName evidence="10">PHB:polyprenyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">COQ2</name>
        <name evidence="11" type="ORF">METBIDRAFT_76173</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of coenzyme Q (CoQ) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>an all-trans-polyprenyl diphosphate + 4-hydroxybenzoate = a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:44504"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9564"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="2.5.1.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Mitochondrion inner membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
            <orientation evidence="2 10">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the UbiA prenyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.39" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713550.1">
        <property type="nucleotide sequence ID" value="XM_018858485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY3"/>
    <dbReference type="GeneID" id="30031461"/>
    <dbReference type="OrthoDB" id="18170at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008412">
        <property type="term" value="F:4-hydroxybenzoate polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13959">
        <property type="entry name" value="PT_UbiA_COQ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1780:FF:000001">
        <property type="entry name" value="4-hydroxybenzoate octaprenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.140:FF:000003">
        <property type="entry name" value="4-hydroxybenzoate polyprenyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01635">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006370">
        <property type="entry name" value="HB_polyprenyltransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039653">
        <property type="entry name" value="Prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01474">
        <property type="entry name" value="ubiA_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048:SF28">
        <property type="entry name" value="4-HYDROXYBENZOATE POLYPRENYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11048">
        <property type="entry name" value="PRENYLTRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="334" mass="37189" checksum="3440BD0DD282EA71" modified="2016-10-05" version="1">MSLNKYEASRIDQIPVFEQNELEAARLARLANLGWLRNLPEKWVPYAELMRLEKPVGTFLLLIPSLWGITMAAYGIMAPLATTVSAIALFSVGALIMRGAGCTINDILDRNLDNKVARTVERPIASGRVSVPQAVGFLAAQCFAGLGVLLSLPFECFYLGALSLPFVFTYPLFKRFTYYPQVMISITFSWGCLLGFPAVLAPLNLLVAAPLFLSNFLWSMIYDSIYAHQDKRYDIKAGIKSTALAWGENTKPILYGLYALQVSSFLTAGFVNSMGPFFYLGAAWGFQRLFSQIKKVDLDTPSSCWAMFTGNIKTGWILWFGMFIDYLVKIGGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG20</accession>
    <name>A0A1A0HG20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF1760-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_76006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713323.1">
        <property type="nucleotide sequence ID" value="XM_018858460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG20"/>
    <dbReference type="STRING" id="869754.A0A1A0HG20"/>
    <dbReference type="GeneID" id="30031436"/>
    <dbReference type="OrthoDB" id="5396786at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013877">
        <property type="entry name" value="YAP-bd/ALF4/Glomulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040347">
        <property type="entry name" value="YBP1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28020:SF1">
        <property type="entry name" value="YAP1-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08568">
        <property type="entry name" value="Kinetochor_Ybp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="646" mass="72419" checksum="C64D129FB2CBAF70" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG73</accession>
    <name>A0A1A0HG73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76021</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713339.1">
        <property type="nucleotide sequence ID" value="XM_018858463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG73"/>
    <dbReference type="STRING" id="869754.A0A1A0HG73"/>
    <dbReference type="GeneID" id="30031439"/>
    <dbReference type="OrthoDB" id="1405595at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="53"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="425"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="655"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="666"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1156" mass="132491" checksum="4DB582CAC7816E80" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG96</accession>
    <name>A0A1A0HG96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_76052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713383.1">
        <property type="nucleotide sequence ID" value="XM_018858469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG96"/>
    <dbReference type="STRING" id="869754.A0A1A0HG96"/>
    <dbReference type="GeneID" id="30031445"/>
    <dbReference type="OrthoDB" id="201656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052585">
        <property type="entry name" value="Lipid_raft_assoc_Zn_ADH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43482">
        <property type="entry name" value="PROTEIN AST1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43482:SF1">
        <property type="entry name" value="PROTEIN AST1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="613" mass="69333" checksum="E4831039BB39C105" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGI9</accession>
    <name>A0A1A0HGI9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_9564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713765.1">
        <property type="nucleotide sequence ID" value="XM_018859409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGI9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGI9"/>
    <dbReference type="GeneID" id="30032384"/>
    <dbReference type="OrthoDB" id="7763451at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343:SF10">
        <property type="entry name" value="RNA-BINDING REGION RNP-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32343">
        <property type="entry name" value="SERINE/ARGININE-RICH SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="28530" checksum="ABA3B2A9A370CB23" modified="2016-10-05" version="1">MSAVTVSHVSTAVELYKLQEFFSFCGNVLSINELDTDAEGFKTYQVNFTLQKAVETALLLNDAELEDVSVKVTKSTLPTYEEAAESGAEVSDNKIQSEKDEAIITGDDSYDDISQEEKPKLAILAQLLASGYKVSDDLIDKAIKFDGQQGISTKFKSFLTDLDSKYLHTQDPESVAAKNLNKAQSLLSSLTNTVQKSSYLQKLQHYFDKASSSPYGAKVHDFYKQVSQEVADVHREATRLLEIKRANASSSAGEQPEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGL5</accession>
    <name>A0A1A0HGL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome P450</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_99409</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713616.1">
        <property type="nucleotide sequence ID" value="XM_018859495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGL5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGL5"/>
    <dbReference type="GeneID" id="30032470"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11070">
        <property type="entry name" value="CYP56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050121">
        <property type="entry name" value="Cytochrome_P450_monoxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24305:SF223">
        <property type="entry name" value="CYTOCHROME P450-DIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291928" evidence="6">
        <location>
            <begin position="19"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="483" mass="55419" checksum="D1403D4DF6F5120B" modified="2016-10-05" version="1">MTWLLVPLLLLLAYFVRLLVLPPSNFPKNIPTIPFYVVFVPVLTDWDQEKVYQHYFRAKMEKYGAVKMYFGSRWNILVSRPEYIAQVMRNNDVFEKSGNNEKIPHAVVLEYLGDNIISAGNERWRKYRKVMTNSILFPDTLSMDGHLARLMSALDQKAQEKTAMVVPGFLQRFFLACIGDCIMGCNLDTEVAGQTILARVRLLKRQIFRPMFMTFPVLDILPIPARTRARVTVQEFKALLRRKILQERSPETALRLGPQLARAVETGDLTQKQFEDNAMISLVAGHENPEMLLASTLYMLAKHPHVQRELRAQLQLLGPDERPDLPLLNAVIFETLRMYPPIGQLVNRITRQPVRLGKDIVIPRGVYVGIHSLITQRDRGHWPDADDFLPGRWGRTEKEVMQSYTLRKSRCEITAFHGRNRACLGEKFALMEVRKCVVAILGRFRFSLDPAWRERVTPAGPIWPEGLSLLISRLADGDEEAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGQ6</accession>
    <name>A0A1A0HGQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="13">PEAMT</shortName>
            <ecNumber evidence="13">2.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_37987</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the first step of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713519.1">
        <property type="nucleotide sequence ID" value="XM_018856939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGQ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGQ6"/>
    <dbReference type="GeneID" id="30029915"/>
    <dbReference type="OrthoDB" id="4583at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004608">
        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016219">
        <property type="entry name" value="Phosphatid-EA_MeTrfase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041611">
        <property type="entry name" value="SKICH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32138:SF0">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17751">
        <property type="entry name" value="SKICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000383">
        <property type="entry name" value="PEAMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51598">
        <property type="entry name" value="SAM_CHO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 13 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="12 13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="442"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="SKICH" evidence="14">
        <location>
            <begin position="665"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="859" mass="98665" checksum="966AA48C7267D8E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGY4</accession>
    <name>A0A1A0HGY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Actin cytoskeleton-regulatory complex protein SLA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_29671</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713623.1">
        <property type="nucleotide sequence ID" value="XM_018855456.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HGY4"/>
    <dbReference type="GeneID" id="30028432"/>
    <dbReference type="OrthoDB" id="26539at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990964">
        <property type="term" value="C:actin cytoskeleton-regulatory complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140224">
        <property type="term" value="C:SLAC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140312">
        <property type="term" value="F:cargo adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09532">
        <property type="entry name" value="SAM_SLA1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11773">
        <property type="entry name" value="SH3_Sla1p_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11775">
        <property type="entry name" value="SH3_Sla1p_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000094">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.700:FF:000001">
        <property type="entry name" value="Actin cytoskeleton-regulatory complex protein SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.700">
        <property type="entry name" value="SLA1 homology domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056996">
        <property type="entry name" value="PH_SLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007131">
        <property type="entry name" value="SHD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035800">
        <property type="entry name" value="Sla1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035821">
        <property type="entry name" value="Sla1_SH3_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF19">
        <property type="entry name" value="ACTIN CYTOSKELETON-REGULATORY COMPLEX PROTEIN SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24081">
        <property type="entry name" value="PH_SLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03983">
        <property type="entry name" value="SHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="75"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="410"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="184"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="480"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="617"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="781"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1215"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="203"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="621"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="647"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="793"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1216"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1226"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1249"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA23142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1292" mass="138579" checksum="2BD1ED2CC00F0EFB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH07</accession>
    <name>A0A1A0HH07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hap4 transcription factor heteromerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_9438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713614.1">
        <property type="nucleotide sequence ID" value="XM_018859391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH07"/>
    <dbReference type="STRING" id="869754.A0A1A0HH07"/>
    <dbReference type="GeneID" id="30032366"/>
    <dbReference type="OrthoDB" id="5374328at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018287">
        <property type="entry name" value="Hap4_TF_heteromerisation"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10297">
        <property type="entry name" value="Hap4_Hap_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hap4 transcription factor heteromerisation" evidence="3">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="562" mass="59530" checksum="80685899E1450441" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH08</accession>
    <name>A0A1A0HH08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_36378</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713762.1">
        <property type="nucleotide sequence ID" value="XM_018856731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH08"/>
    <dbReference type="STRING" id="869754.A0A1A0HH08"/>
    <dbReference type="GeneID" id="30029707"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990333">
        <property type="term" value="C:mitotic checkpoint complex, CDC20-MAD2 subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040020">
        <property type="term" value="P:regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="9">
        <location>
            <begin position="137"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="188"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="266"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="402"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="50869" checksum="7E5E9C776DE9B248" modified="2016-10-05" version="1">MSPPKTAKLRRSTSLLTLQEATTSDRFIPSHKNSTTSKLTAASERPHPNASPQTHIKAQTNKIYQHHVAEACGLDVNSRILSYRPLPPERSKPLNLFSHITLGSLCKALERTTGKSLRPSLASERAKKIPTAPDRVLDAPNIVDDFYLNLVSWSLTNLIVIGLEDAVYVWNASTGSVGLLCETPGSLITSLRWSGDGSYISIGKDDGSIEIWDIETNSRLRTLASSSTGRVASHCWLLHLLTSGSKEGTIYHSDVRVSSHLVGKRENTHLAEVCGIEYRGDGQQFCSGGNDNVVAIWDARNMSGDALFQKTNHRAAVKAMAWCPYQSLLLATGGGTSDKSIHFWNTSTGARVNLIETESQISSLNWGYAKGTGYEVVATHGYPSNSISLFNYPTLQKTGEVLQAHDSRILNGCLSPDGTTLATVSGDENLKFWSLFDFYRQARPDAEESFASNDGKHMGKLMNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH40</accession>
    <name>A0A1A0HH40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9463</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713644.1">
        <property type="nucleotide sequence ID" value="XM_018859397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH40"/>
    <dbReference type="STRING" id="869754.A0A1A0HH40"/>
    <dbReference type="GeneID" id="30032372"/>
    <dbReference type="OrthoDB" id="4068492at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053278">
        <property type="entry name" value="Pre-60S_factor_ECM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022784">
        <property type="entry name" value="Ribosome_bgen_Alb1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28280">
        <property type="entry name" value="SHUTTLING PRE-60S FACTOR ECM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28280:SF1">
        <property type="entry name" value="SHUTTLING PRE-60S FACTOR ECM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09135">
        <property type="entry name" value="Alb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="151"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="186" mass="20982" checksum="57C39288F6E2ADB9" modified="2016-10-05" version="1">MAKKVSKHSRAARRGEIDVGGEAKALENLPRDENDDVRKHIIRTTIKNENLLAKKMELEKVKKKGNKKLSHTFKHKTEKNSRLEGVLAGKIQQSIEKARYVQTARKSGWDQINKSIVIRTDLTEAPAEKAGEAVSEKEAQQKEEDEYVKLFFGDQATTEEPPQDEAPVAKPKPSGNIFDMLEETDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH80</accession>
    <name>A0A1A0HH80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear pore complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_29850</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the nucleoporin Nup84/Nup107 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713836.1">
        <property type="nucleotide sequence ID" value="XM_018855495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH80"/>
    <dbReference type="STRING" id="869754.A0A1A0HH80"/>
    <dbReference type="GeneID" id="30028471"/>
    <dbReference type="OrthoDB" id="3098at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007252">
        <property type="entry name" value="Nup84/Nup107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003:SF2">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13003">
        <property type="entry name" value="NUP107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04121">
        <property type="entry name" value="Nup84_Nup100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="793" mass="90461" checksum="9328E87FAD0CC6A8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHJ2</accession>
    <name>A0A1A0HHJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_36414</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713828.1">
        <property type="nucleotide sequence ID" value="XM_018856736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHJ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHJ2"/>
    <dbReference type="GeneID" id="30029712"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045540">
        <property type="entry name" value="YegS/DAGK_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF31">
        <property type="entry name" value="ACYLGLYCEROL KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19279">
        <property type="entry name" value="YegS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="5">
        <location>
            <begin position="114"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="483" mass="53174" checksum="7DE14DB2EA08478D" modified="2016-10-05" version="1">MSNGLSNTSAVSEKALVGEYGITIVKPTASRATYGVLCFGSAPVSNATENLHVPFKNVLFCENSSSSEVDLTYAVVDGEKIEIHQDCLDVGAYTLDSLPDMAHHIMLLSYQQGSKIPRVMIVLNNFGGQGNARKIYQSQILPVLQAAHVEYAYMETEYSGHASDIARELELSEYDIIACCSGDGVPHEVMNGFYQRADREQAFEQIAITQLPCGSGNALSLSSFGTNSAGIAAVKMLKLARKRMDAMAVTQGSTTKISFLSQAYGIIADSDIGTEHLRWMGPMRFDFGVTQRLITNTTYPCDLFVKYVLRDNDEIQGHYNKHRFNPVDDRGATELELVARFPTFDVLPPADWEQLPREITDNLSIFYVGKMPYVLSDAQFFPAALPNDGCMDMIITSAKTSFFKMAKLLFSIDKGRHIDSPDVLHAKISAYRLVPRIGAENHFISVDGESFPFEPLQVEVLSRTLTVLLDEGAFVDTCLTGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHP0</accession>
    <name>A0A1A0HHP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_76420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713878.1">
        <property type="nucleotide sequence ID" value="XM_018858523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHP0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHP0"/>
    <dbReference type="GeneID" id="30031499"/>
    <dbReference type="OrthoDB" id="420195at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="2">
        <location>
            <begin position="509"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="740" mass="84367" checksum="B06ECBC450869636" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHV5</accession>
    <name>A0A1A0HHV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-14 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_36843</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713943.1">
        <property type="nucleotide sequence ID" value="XM_018856787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHV5"/>
    <dbReference type="GeneID" id="30029763"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904352">
        <property type="term" value="P:positive regulation of protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000185">
        <property type="term" value="P:regulation of phosphate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327">
        <property type="entry name" value="RING FINGER PROTEIN 127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23327:SF51">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR OF YEAST FORM ADHERENCE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15227">
        <property type="entry name" value="zf-C3HC4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="369"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="53414" checksum="0BAE50B3477ADE36" modified="2016-10-05" version="1">MKFAKVLQQTLIEEDLPQEWVEAAIQYKVLKKCISRIVKELAFLGLSQADLKLLLQDEELDSSIEVATNEATPNNPIVAHYTLKKANSHIVPYLKIVLNNNESNQYSPDRIQELAGQIRAKLHSVMSTDDEDHHIIELKEDEDLFVLSPTTSIREEDAESIVKVENHNEIVIMLNSDSKFFRMLSLELEKLDEIRTSEEKKLIDEIDALSNTVLLLTRKKSDLYTWREFFRIYLDSEVYFRYNETSQSHLERSSEQIKRNLQDFADRAKKARISERFKSKLAVSAYNSFLQMNTQLLKIILYQSINSTALRKILKKFDKRTCLNVSGRFPDLISKDHVFVHGSSVAQSICFVMQNKLLTLVPQLDDYTCPICMSIAFKPIRLDCGHIFCVRCLVKTKQRGRTDCPLCRYHDAIAIADSSNLDLDAMELIKKNFPREVKEKLKEIDQERYNELVKHKNCVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI51</accession>
    <name>A0A1A0HI51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sec7-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_36814</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">COPI-coated vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714043.1">
        <property type="nucleotide sequence ID" value="XM_018856780.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HI51"/>
    <dbReference type="GeneID" id="30029756"/>
    <dbReference type="OrthoDB" id="18431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030663">
        <property type="term" value="C:COPI-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140455">
        <property type="term" value="P:cytoplasm protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000002">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF375">
        <property type="entry name" value="LD29171P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="7">
        <location>
            <begin position="564"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="535"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1465"/>
            <end position="1484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="535"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1693" mass="191065" checksum="BCC712F1AEFC53BB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIB3</accession>
    <name>A0A1A0HIB3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bola-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714226.1">
        <property type="nucleotide sequence ID" value="XM_018856506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIB3"/>
    <dbReference type="GeneID" id="30029482"/>
    <dbReference type="OrthoDB" id="4983at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071281">
        <property type="term" value="P:cellular response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045115">
        <property type="entry name" value="BOL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735:SF27">
        <property type="entry name" value="BOLA-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12735">
        <property type="entry name" value="BOLA-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="9486" checksum="ABB97F5A8A0B6931" modified="2016-10-05" version="1">MLLTANELKDIISERLEAPLVQVEDMSGGCGQAFAVIIVTPKFQGKNKLMRHRMVNTALKTEIASIHAFTQKGFTPEEWKAQGGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIC0</accession>
    <name>A0A1A0HIC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD repeat-containing protein JIP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">WD repeat-containing protein jip5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_30281</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat WDR55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714395.1">
        <property type="nucleotide sequence ID" value="XM_018855599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIC0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIC0"/>
    <dbReference type="GeneID" id="30028575"/>
    <dbReference type="OrthoDB" id="2288928at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924">
        <property type="entry name" value="UTP15 U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924:SF31">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN JIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="398"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="497"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="501"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="515"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="535" mass="59072" checksum="D60557B01CEFA487" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIE9</accession>
    <name>A0A1A0HIE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30302</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714425.1">
        <property type="nucleotide sequence ID" value="XM_018855606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIE9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIE9"/>
    <dbReference type="GeneID" id="30028582"/>
    <dbReference type="OrthoDB" id="3824970at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14424">
        <property type="entry name" value="CUE_Cue1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20112" checksum="33A5200EC628E1E0" modified="2016-10-05" version="1">MDNSTIIFVATLAIVLVILRWFITPIPQSVPDEFNIPDPARSSVASGTGRHRRQVTESMIEVVQAIGPQLSISQIRHSLENTGSVEATIEILMESGNLPFPPGENFDTSTSATSDHSDVQTRCTKSLLEKYEVEGEPRKSFATAGKWGKDENERLSLLRSRKAELILRARENMKATICKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIJ6</accession>
    <name>A0A1A0HIJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Endopolyphosphatase</fullName>
            <ecNumber evidence="3 12">3.6.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_76695</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolysis of inorganic polyphosphate (polyP) chains of many hundreds of phosphate residues into shorter lengths.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[phosphate](n+1) + n H2O = (n+1) phosphate + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22452"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the endopolyphosphatase PPN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.10" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714188.1">
        <property type="nucleotide sequence ID" value="XM_018858573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIJ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIJ6"/>
    <dbReference type="GeneID" id="30031549"/>
    <dbReference type="OrthoDB" id="348678at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012358">
        <property type="entry name" value="EndopolyPtase_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF55">
        <property type="entry name" value="ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027093">
        <property type="entry name" value="EndopolyPtase_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 12" id="KW-0926">Vacuole</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR027093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="641" mass="74077" checksum="26993C4BA4E92ED4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIL2</accession>
    <name>A0A1A0HIL2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA/RNA polymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_34699</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714159.1">
        <property type="nucleotide sequence ID" value="XM_018856513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIL2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIL2"/>
    <dbReference type="GeneID" id="30029489"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000008">
        <property type="entry name" value="DNA polymerase eta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="10">
        <location>
            <begin position="44"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="639"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="655"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="750" mass="85879" checksum="4375C0255C4A9E03" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIS9</accession>
    <name>A0A1A0HIS9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714545.1">
        <property type="nucleotide sequence ID" value="XM_018856851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIS9"/>
    <dbReference type="GeneID" id="30029827"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291818" evidence="1">
        <location>
            <begin position="23"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6958" checksum="9D45DDC9672940F8" modified="2016-10-05" version="1" fragment="single">KKIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPTDFYRCYFRPRGLRELYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIV9</accession>
    <name>A0A1A0HIV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histone deacetylase complex subunit SAP30 Sin3 binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10145</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714422.1">
        <property type="nucleotide sequence ID" value="XM_018853695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIV9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIV9"/>
    <dbReference type="GeneID" id="30026671"/>
    <dbReference type="OrthoDB" id="510958at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000219">
        <property type="term" value="P:positive regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="2">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="153" mass="17512" checksum="8930882DD8AFB598" modified="2016-10-05" version="1">MPPRVQNKDSTSESESKATLTSRAANKARNAVAAAAQLELLSKHIHSNGPKDKPSIHPLDFQSFSEETLNNYLRKYSLNYPDVRSLNENILNSEIGKKTYSYKRATHGNKISKPELASNLQKHFTLMNIRENEVITNFLYRVKNQDKEFKLTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ62</accession>
    <name>A0A1A0HJ62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_36953</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714536.1">
        <property type="nucleotide sequence ID" value="XM_018856807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ62"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ62"/>
    <dbReference type="GeneID" id="30029783"/>
    <dbReference type="OrthoDB" id="206213at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008413">
        <property type="term" value="F:8-oxo-7,8-dihydroguanosine triphosphate pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015938">
        <property type="term" value="P:coenzyme A catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03426">
        <property type="entry name" value="NUDIX_CoAse_Nudt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF24">
        <property type="entry name" value="PEROXISOMAL COENZYME A DIPHOSPHATASE NUDT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="219"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="361" mass="40474" checksum="568F7DF8CC585C12" modified="2016-10-05" version="1">MDALHHAHHYSAQHYQGEALSVWHRMPVARRALVFILLFIGSVGEIRVVLTKRLSQLRNFPGHISLPGGKADHGLELPWMVARREMSEEIGLSENDAELHREYGVVIDQVTELPCYLSRTFSAVKPVVGFLNTGRGVDAALLRMRLRVNPGECSSIFLCPLHDFLYPVSDAPAKEALARTHHPVKWGGLPWNLRSYTFPQANPGEVHWLRGYDDLSATEDEDSAAELEASLAGPDPESCPTQDSQRPPKKPKKDLSGWGNLGSRRDSVSNEKIYDVWGLTANILHDLAEVVYLPPHRRSPGKQHGEEELIWSVWHHGQQMHLKERTPAEAALIRAGPADPASFGDVLPRTEFNRLKRIYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA1</accession>
    <name>A0A1A0HJA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transport protein particle component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714557.1">
        <property type="nucleotide sequence ID" value="XM_018856885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA1"/>
    <dbReference type="GeneID" id="30029861"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="217" mass="23920" checksum="5A9767AE507D5DD0" modified="2016-10-05" version="1">MGSATADVLESQTYVSRVCVGLLLQELVPTSVRVLLRLSEMKSVEKSPSGEAQTRIDTPLSSIVGDMPGTTAVFGSELLDSEDVVLRVEKYGFDLGLRLTEVLMYKAETKQKIVDILEVMKFICRDAWRTLYGKQMDNLRTNHRGTFVLIDNQYKVTAGFSSEKGKSDAVAKARAYTYLPCGIIRGILLSFGVDAYVSADIAQFPSVAFNIQTTINN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA9</accession>
    <name>A0A1A0HJA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho GTPase activation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_36529</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714453.1">
        <property type="nucleotide sequence ID" value="XM_018856752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA9"/>
    <dbReference type="GeneID" id="30029728"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05137">
        <property type="entry name" value="RasGAP_CLA2_BUD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023152">
        <property type="entry name" value="RasGAP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00509">
        <property type="entry name" value="RAS_GTPASE_ACTIV_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="373"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="4">
        <location>
            <begin position="590"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="709"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="711"/>
            <end position="730"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1164" mass="132567" checksum="7467900C2235AC51" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF1</accession>
    <name>A0A1A0HJF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Co-chaperone HscB C-terminal oligomerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_37876</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HscB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714607.1">
        <property type="nucleotide sequence ID" value="XM_018856925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJF1"/>
    <dbReference type="GeneID" id="30029901"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="entry name" value="HscB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="HSC20 (HSCB), C-terminal oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Co-chaperone HscB C-terminal oligomerisation" evidence="3">
        <location>
            <begin position="123"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="208" mass="23128" checksum="CD32194C041BF0D3" modified="2016-10-05" version="1">MHTQDTLFRLFPKLFPAGGPPKDPFQVNLRLLRREYRALQGQNHPDVVMGAAGFGPNTTAGSVNDGASSAINHAYATLRNPYTRAAYIVELQHPEKLDITKDDVLKQFIANVQAASQEHSLDYKALLMTVLEAHEALESATSEADLAALEEENTARIEQSEQSLDALLKQTPVPWEAFIIETIELKYWVNIANGIKDWEPGKPVLLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJT6</accession>
    <name>A0A1A0HJT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acetolactate synthase small subunit mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_77094</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714742.1">
        <property type="nucleotide sequence ID" value="XM_018858638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJT6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJT6"/>
    <dbReference type="GeneID" id="30031614"/>
    <dbReference type="OrthoDB" id="2013116at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053050">
        <property type="entry name" value="ALS_regulatory_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31242:SF2">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="64"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="262"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="290" mass="31493" checksum="1C575357FC34AAAB" modified="2016-10-05" version="1">MLRQARSKVTSSSTSALAYKTLHRNQKRPALPTFDTPNWSADAAVSSILYETPVPSKAPRKQHVLNCLVQNEPGVLSSVSGTLAARGFNIDSLVVCNTAVKDLSRMTVVLRGQDAVVEQARRQIEDLVPVYAVLDYTNAEIVQRELLLARISLLGPEYFQDLIAAHKEYASEGSALPDLAARDSEFHPSNLAPSAALRQKHAHLQHISTIVDKFGGKVVDISDRNVVVELSAKPSRVSSFIQLLHPFGILEIARSGMMALPRTPLEESSPSDEDAVDANDVVDASQLPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX2</accession>
    <name>A0A1A0HJX2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">amidase</fullName>
            <ecNumber evidence="3">3.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_34728</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a monocarboxylic acid amide + H2O = a monocarboxylate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35757"/>
            <dbReference type="ChEBI" id="CHEBI:83628"/>
            <dbReference type="EC" id="3.5.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.4" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714801.1">
        <property type="nucleotide sequence ID" value="XM_018856515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX2"/>
    <dbReference type="GeneID" id="30029491"/>
    <dbReference type="OrthoDB" id="6428749at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072:SF4">
        <property type="entry name" value="AMIDASE C550.07-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46072">
        <property type="entry name" value="AMIDASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001221">
        <property type="entry name" value="Amidase_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="7">
        <location>
            <begin position="102"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="253"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001221-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="574" mass="63639" checksum="9171A6EE96B6A5AE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX8</accession>
    <name>A0A1A0HJX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_26773</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714787.1">
        <property type="nucleotide sequence ID" value="XM_018855327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX8"/>
    <dbReference type="GeneID" id="30028303"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1350">
        <property type="entry name" value="Pneumovirus matrix protein 2 (M2), zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045072">
        <property type="entry name" value="MKRN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224:SF58">
        <property type="entry name" value="C3H1-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11224">
        <property type="entry name" value="MAKORIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="65" mass="7049" checksum="5CD6E6E537B5822A" modified="2016-10-05" version="1" fragment="single">STANKNLSHVPCKFFKQGICQAGKSCPFSHNVDGTLSADKLPCKYFQKGNCKFGMKCALAHISPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKI3</accession>
    <name>A0A1A0HKI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glucocorticoid receptor-like (DNA-binding domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_25191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714883.1">
        <property type="nucleotide sequence ID" value="XM_018855289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKI3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKI3"/>
    <dbReference type="GeneID" id="30028265"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="57" mass="6587" checksum="B63E5037E4575065" modified="2016-10-05" version="1" fragment="single">PVCRNCKTQTTPLWRRDETGQVLCNACGLFLKLHGRPRPISLKTDTIKLRNRIKQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ54</accession>
    <name>A0A1A0GZ54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">25S rRNA (Uridine(2843)-N(3))-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_48087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709305.1">
        <property type="nucleotide sequence ID" value="XM_018858256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ54"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ54"/>
    <dbReference type="GeneID" id="30031232"/>
    <dbReference type="OrthoDB" id="6419443at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021463">
        <property type="entry name" value="Methyltransf_34"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11312">
        <property type="entry name" value="Methyltransf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA17008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="320" mass="36153" checksum="7958AE72C63B9A4A" modified="2016-10-05" version="1">MSLRALNYDQRPLKKPSTFSSANDNIPFHHENTLAPFKVIDLFLEQFQHVLAAEDLPEMIQAVKAQLYQRDYLSAFDCDDKRLAYAARWTPARALAYSSLFASCPEISELFSDPNKKSRVLCVGGGAASELVGLASVFCQLKIPNGDTPSSLHMDVIDIADWSLIVNNVTSYVRNKWLYSPSKLSAQFKLGDVLAQTPEQLHLSELDLVTLLFTTNELFSEKRTETIKFLHSLSAHCRKGSLLLITESAGSYSHITVGLKEFPVQFLIDTILVGKPGVGNGPWEIVNQSESCWYRINDREVSYPIKLENMRFFYRLYSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Y3</accession>
    <name>A0A1A0H1Y3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_80079</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC43A transporter (TC 2.A.1.44) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709363.1">
        <property type="nucleotide sequence ID" value="XM_018859168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Y3"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Y3"/>
    <dbReference type="GeneID" id="30032144"/>
    <dbReference type="OrthoDB" id="330047at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052599">
        <property type="entry name" value="SLC43A_AATransporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20772:SF2">
        <property type="entry name" value="PROTEIN FMP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="467"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA17968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="549" mass="60701" checksum="8C34E4EDB8DC228E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H213</accession>
    <name>A0A1A0H213_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709466.1">
        <property type="nucleotide sequence ID" value="XM_018858232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H213"/>
    <dbReference type="STRING" id="869754.A0A1A0H213"/>
    <dbReference type="GeneID" id="30031208"/>
    <dbReference type="OrthoDB" id="10264707at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052742">
        <property type="entry name" value="Mito_N-acetyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138">
        <property type="entry name" value="ACETYLTRANSFERASE, GNAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43138:SF1">
        <property type="entry name" value="N-ACETYLTRANSFERASE ACA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="70"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="219" mass="25334" checksum="063025596CFB45F3" modified="2016-10-05" version="1">MTYDIDHLKTLPFADRTYATTPRVKDNFIFNLAKTDETVTLITAHDADEMPSGLLHLAWNEMNFVIREGRTYPHYLEFSYDDFLAYFFEGFAAILVEGRYESTWESESQDFWDQKYLGHFYIKPNYIGRCLHVCNAGFVVNHGRRGLGLGKELGKQYLTIAPKLGYVYLVFNLVFETNSASYKIWESLGFERIGYVKNVAMLKGEDKLVGAFMYGKDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H224</accession>
    <name>A0A1A0H224_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_33762</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709400.1">
        <property type="nucleotide sequence ID" value="XM_018856394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H224"/>
    <dbReference type="STRING" id="869754.A0A1A0H224"/>
    <dbReference type="GeneID" id="30029370"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="PITH" evidence="3">
        <location>
            <begin position="140"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA18005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="329" mass="37055" checksum="FAB48A2E1F061D24" modified="2016-10-05" version="1">MAEALEIQFVKNEIEFNRLLTEKKYLMVNFTASWCGPCQASKPTVDGMYQHPKYKKIEFLRVDIDSCQAVARKYEISSVPTFLFFESLKETERVRGFSPKVKEALDALAEKANTDHSVVDRENQQASGLLGSGAEYRKEIAKFIPKGFEVVNDAIHFGELVALNIMPLYKKTSDVKLLFKPASENTTVYSDADSQALFFVPLNHICKVYSILVKISKPAALESTELDLDELADESQSPALVKVWANKPSILSFEDCTGSDALHEERLKPDLEPGWHEIKLRYVRFQSVQNLNIFVEGADEDAHTIIDKVVIVGLTGESMEHTAIQQDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H234</accession>
    <name>A0A1A0H234_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
            <ecNumber evidence="2">1.14.11.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_89221</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(6),N(6),N(6)-trimethyl-L-lysyl(9)-[histone H3] + 2 2-oxoglutarate + 2 O2 = N(6)-methyl-L-lysyl(9)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:60200"/>
            <dbReference type="Rhea" id="RHEA-COMP:15538"/>
            <dbReference type="Rhea" id="RHEA-COMP:15542"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="1.14.11.66"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the JHDM3 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.66" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709410.1">
        <property type="nucleotide sequence ID" value="XM_018859297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H234"/>
    <dbReference type="STRING" id="869754.A0A1A0H234"/>
    <dbReference type="GeneID" id="30032272"/>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140684">
        <property type="term" value="F:histone H3K9me2/H3K9me3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="8">
        <location>
            <begin position="22"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="9">
        <location>
            <begin position="212"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="450"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="117"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA18015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="754" mass="85404" checksum="BA8040EA0A478C0A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4E3</accession>
    <name>A0A1A0H4E3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_47459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709479.1">
        <property type="nucleotide sequence ID" value="XM_018858167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4E3"/>
    <dbReference type="GeneID" id="30031143"/>
    <dbReference type="OrthoDB" id="3983163at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024645">
        <property type="entry name" value="Mitochondr_Som1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11093">
        <property type="entry name" value="Mitochondr_Som1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="126" mass="14720" checksum="CA8EA7EDE096F648" modified="2016-10-05" version="1">MSPPTPVRTRAEMHSIYKEQLTNPGKYQCTLKSLSQLECTFKISPTNSVLETICIPFKRVFQRCLQPYTRVVDGKKQRGERWINIEVTDLETNDGIRSKHTDEIQRFLKAEVDLAKWLESQMEEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4K0</accession>
    <name>A0A1A0H4K0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_223592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709501.1">
        <property type="nucleotide sequence ID" value="XM_018855161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4K0"/>
    <dbReference type="GeneID" id="30028137"/>
    <dbReference type="OrthoDB" id="60822at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36417:SF2">
        <property type="entry name" value="SELENOPROTEIN DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G05220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="128" mass="14834" checksum="392198BF9E84AF61" modified="2016-10-05" version="1">MYPKVTIEYCARCKWHNRAVWYLQEILQTFSDPEKNLVAEVAVRPSYDKPGIFVIEISTQDDVSKIIYCRRLKKSGLPQTESYHYDGFPDSKLLKNLIRDSLFPEHGLGHLDGKADLLTDCIPCRENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4L1</accession>
    <name>A0A1A0H4L1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709511.1">
        <property type="nucleotide sequence ID" value="XM_018854420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4L1"/>
    <dbReference type="GeneID" id="30027396"/>
    <dbReference type="OrthoDB" id="4094525at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="116" mass="12578" checksum="CA69106C016BD147" modified="2016-10-05" version="1">MGNSNHKPTGTKSTKLGKSSSASRTVPPKASVLKSKLKLRSKRTKAQIEKLNQDESTIASIHAALLARGQVPKEVKILDAQSLREGMLKDQQTKLQSEKAEKDLASQLELITGMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4U9</accession>
    <name>A0A1A0H4U9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Brix-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_13848</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BRX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709639.1">
        <property type="nucleotide sequence ID" value="XM_018854435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4U9"/>
    <dbReference type="STRING" id="869754.A0A1A0H4U9"/>
    <dbReference type="GeneID" id="30027411"/>
    <dbReference type="OrthoDB" id="1638493at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000464">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000465">
        <property type="term" value="P:exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10480:FF:000003">
        <property type="entry name" value="Ribosome biogenesis protein BRX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="7">
        <location>
            <begin position="26"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="33195" checksum="7102877301CC0096" modified="2016-10-05" version="1">MSAIYKALQSKSSKDTSEKAKHINRQRVLVISSRGVTYRHRHLINDLTQLMPHARKEPKFDSKKNLWQLNEVAELYNCNNIFFFEARKHQDLYLWISKPPNGPTLKFYIQNMHTLDELNFTGNCLKGSRPILSFDKSFTDSPHHELMKEMFLHTFGVPPNARKSKPFVDHVMTFSIVDNKIWVRNYQISETELLKESEVANIDADSLSLVEIGPRFVMTLITILEGSFGGPKIYENKQYVSPNFARAQIKQKAAEQARSRANAAVDRKVKLRDQVLKVDPLSNASLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X5</accession>
    <name>A0A1A0H4X5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Peptide hydrolase</fullName>
            <ecNumber evidence="15">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_47460</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">May be involved in vacuolar sorting and osmoregulation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Vacuole membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the peptidase M28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709535.1">
        <property type="nucleotide sequence ID" value="XM_018858168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X5"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X5"/>
    <dbReference type="GeneID" id="30031144"/>
    <dbReference type="OrthoDB" id="76293at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03875">
        <property type="entry name" value="M28_Fxna_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048024">
        <property type="entry name" value="Fxna-like_M28_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053975">
        <property type="entry name" value="PFF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053976">
        <property type="entry name" value="PFF1_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF58">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22250">
        <property type="entry name" value="PFF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22251">
        <property type="entry name" value="PFF1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 21" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 15 21" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <keyword evidence="10 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="465"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="503"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="537"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="604"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="647"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="671"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="18">
        <location>
            <begin position="164"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease transmembrane" evidence="20">
        <location>
            <begin position="566"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar membrane protease C-terminal" evidence="19">
        <location>
            <begin position="697"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF22251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA19000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="978" mass="110051" checksum="3A6E164F06FC915A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Y6</accession>
    <name>A0A1A0H4Y6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="12">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_33603</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="12">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AdoMet synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709507.1">
        <property type="nucleotide sequence ID" value="XM_018856346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Y6"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Y6"/>
    <dbReference type="GeneID" id="30029322"/>
    <dbReference type="OrthoDB" id="5852090at2759"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006555">
        <property type="term" value="P:methionine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18079">
        <property type="entry name" value="S-AdoMet_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000001">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000003">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000004">
        <property type="entry name" value="S-adenosylmethionine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00086">
        <property type="entry name" value="S_AdoMet_synth1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022631">
        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022630">
        <property type="entry name" value="S-AdoMet_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022629">
        <property type="entry name" value="S-AdoMet_synt_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022628">
        <property type="entry name" value="S-AdoMet_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002133">
        <property type="entry name" value="S-AdoMet_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022636">
        <property type="entry name" value="S-AdoMet_synthetase_sfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01034">
        <property type="entry name" value="metK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11964">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02773">
        <property type="entry name" value="S-AdoMet_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02772">
        <property type="entry name" value="S-AdoMet_synt_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00438">
        <property type="entry name" value="S-AdoMet_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000497">
        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55973">
        <property type="entry name" value="S-adenosylmethionine synthetase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00376">
        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00377">
        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="14">
        <location>
            <begin position="4"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="15">
        <location>
            <begin position="117"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase C-terminal" evidence="16">
        <location>
            <begin position="239"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA18972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="382" mass="42118" checksum="8CC8C41A4D5DAEEF" modified="2016-10-05" version="1">MPEKFLFTSESVGEGHPDKICDQVSDAILDACLAEDPLSKVACETAAKTGLIMVFGEITTKANLDYQKIIRDTIKHIGYDHSDKGFDYKTCNVLVAIEQQSPDIAQGLHYEKALEELGAGDQGIMFGYATDETEEKLPLTILLSHKLNKALADARRSGELAWLRPDTKTQVTVEYEKEGGAVKPLRVDTVVISTQHADDISTEDLREKILEQIIKKTIPAHLLDDKTIYHIQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVNAGLARRALVQFSYAIGVAEPLSIHVDTYGTSKYTSEQLVEIIRNNFDLRPGVIVKELNLARPIYFNTASYGHFTNQENSWEKPKELKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5T7</accession>
    <name>A0A1A0H5T7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_79905</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709928.1">
        <property type="nucleotide sequence ID" value="XM_018859146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5T7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5T7"/>
    <dbReference type="GeneID" id="30032122"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF73">
        <property type="entry name" value="METABOLITE TRANSPORT PROTEIN YDL199C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="406"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="80"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="570" mass="63747" checksum="3BA820C28F0563AF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5U0</accession>
    <name>A0A1A0H5U0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA-splicing factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45677</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in spliceosome maturation and the first step of pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the SPP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709982.1">
        <property type="nucleotide sequence ID" value="XM_018857945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5U0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5U0"/>
    <dbReference type="GeneID" id="30030921"/>
    <dbReference type="OrthoDB" id="5577072at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045166">
        <property type="entry name" value="Spp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818">
        <property type="entry name" value="G PATCH AND KOW-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818:SF2">
        <property type="entry name" value="G-PATCH DOMAIN AND KOW MOTIFS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Spp2/MOS2 G-patch" evidence="6">
        <location>
            <begin position="136"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="15"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="213" mass="23064" checksum="84A3AABEDB31985A" modified="2016-10-05" version="1">MSGFSFSLKTNKVTKDKKVKPGLTRRTVPKKNALFHDEDATTDKKTSIDAFDSSKGAMLGSTAVSETPVLIIEPVNRTHRLRRPSISAYTGDDSENADTSKLLAEEKARISLLKGELYQETSSKVIASHSEDGDAEAAPEDYEAVPVDQFGAALLRGMGWSGEPEPNNQAGQDVSHRKQGAVLGIGAKSIDKDLEAELLSKKNLGVPLIKRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6B0</accession>
    <name>A0A1A0H6B0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-ureidoglycine aminohydrolase cupin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710152.1">
        <property type="nucleotide sequence ID" value="XM_018858023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6B0"/>
    <dbReference type="GeneID" id="30030999"/>
    <dbReference type="OrthoDB" id="4985585at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02228">
        <property type="entry name" value="cupin_EutQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010424">
        <property type="entry name" value="EutQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008579">
        <property type="entry name" value="UGlyAH_Cupin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169:SF1">
        <property type="entry name" value="ACETATE KINASE EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169">
        <property type="entry name" value="ETHANOLAMINE UTILIZATION PROTEIN EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05899">
        <property type="entry name" value="Cupin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="(S)-ureidoglycine aminohydrolase cupin" evidence="2">
        <location>
            <begin position="41"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="121" mass="13543" checksum="AEE20C62547B9A76" modified="2016-10-05" version="1">MPMQYKPSAEGKEVRPPQIPSPGNNSFLGDIFTSDAPKEQQISCGFYKQEAGEPLVYKYDYDEMKIVLEVEGEYTFTDETGYKVSVKPGDVFYFPKGTTITFETTGYGYAFFTGLRPNGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L5</accession>
    <name>A0A1A0H6L5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Increased recombination centers protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_13309</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Is probably involved in a pathway contributing to genomic integrity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IRC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710081.1">
        <property type="nucleotide sequence ID" value="XM_018854338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6L5"/>
    <dbReference type="GeneID" id="30027314"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="210"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26378" checksum="AB44BE39CAC60275" modified="2016-10-05" version="1">MARENALSMLDKTHCAFAAITKVLAEDDIDMSSADEVELKNYKIDFYIEEHEEVSASEVAELVYGETITINYNLKNEEDSVMTIVGLGGAFLDPLSGDPLVNMTANSVEPIILQPEESTHVRQKVTVDFPLRDYILAPNVYVAFKDQLKGILPRGQPVSLSEPPVSLLHPKLLFLELLFLGAIAAYVYQFHRSAVELYFKDIAPETKLMQSSGSASGSWLPKTYQTAQKKPKARKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L6</accession>
    <name>A0A1A0H6L6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_74059</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710199.1">
        <property type="nucleotide sequence ID" value="XM_018858422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6L6"/>
    <dbReference type="GeneID" id="30031398"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF10">
        <property type="entry name" value="CHAPERONE PROTEIN DNAJ A6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="20"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="291" mass="33117" checksum="45B2CB05B3BB60F2" modified="2016-10-05" version="1">MRATHSAITSRRMYSSAQPNLYQVLELLPNASTKDIKMKFKRLSKKYHPDVNAHLSDQEKEANSKRFVEMVLAYDTLKDKKKRKLYDATLLSSPRGGGSMYSRRKPPSAWENEYYGEAKHYSRARASGSYTSHGYNYTRHRVHNFYRASNGENGSQHFTGEHKNRGDRFDVPHFDYTSHLSKQLKFEQRIISKHLSEAEKEAVLRQLAPDGDLSKVSEELITKHLMRQAKNENAARQTHSHQSGAASNPYMYRGPQNSNMDSEESGVGFKALVIAGGAGSAYLLYHLILAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6M9</accession>
    <name>A0A1A0H6M9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA wybutosine-synthesizing protein 4</fullName>
            <ecNumber evidence="5">2.1.1.290</ecNumber>
            <ecNumber evidence="4">2.3.1.231</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_79673</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine + CO2 = wybutosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37119"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="Rhea" id="RHEA-COMP:11847"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73544"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.3.1.231"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-(3-amino-3-methoxycarbonyl)propyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36903"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="Rhea" id="RHEA-COMP:11844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:74275"/>
            <dbReference type="EC" id="2.1.1.290"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.290" evidence="5"/>
    <dbReference type="EC" id="2.3.1.231" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710209.1">
        <property type="nucleotide sequence ID" value="XM_018859110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6M9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6M9"/>
    <dbReference type="GeneID" id="30032086"/>
    <dbReference type="OrthoDB" id="47172at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46529:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13418">
        <property type="entry name" value="Beta-prop_TYW4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="639" mass="71388" checksum="208E264E0F6AEDBC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H761</accession>
    <name>A0A1A0H761_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">intramembrane prenyl-peptidase Rce1</fullName>
            <ecNumber evidence="10">3.4.26.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_44590</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic sidechains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.26.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.26.1" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710263.1">
        <property type="nucleotide sequence ID" value="XM_018857813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H761"/>
    <dbReference type="STRING" id="869754.A0A1A0H761"/>
    <dbReference type="GeneID" id="30030789"/>
    <dbReference type="OrthoDB" id="271604at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039731">
        <property type="entry name" value="Rce1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003675">
        <property type="entry name" value="Rce1/LyrA-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046:SF0">
        <property type="entry name" value="CAAX PRENYL PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13046">
        <property type="entry name" value="PROTEASE U48 CAAX PRENYL PROTEASE RCE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02517">
        <property type="entry name" value="Rce1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="92"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 2/Lysostaphin resistance protein A-like" evidence="12">
        <location>
            <begin position="132"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="271" mass="30261" checksum="BE8497ACB367771E" modified="2016-10-05" version="1">MFFNLLVGAVVASLYVAAIKLSVPVHVVHLERSNPIVIRFRIRRIFVLCGILLFAIPVLSVQLNGASTYGQALRNMGLFPGFTSSHDFVGDVMGIVIAWLKIASLYIGPICAYFYIDAQGWRADFQANFCTLAGIRDHVFAPITEEFVYRAALVAVLAPVLLENSLIFYSPLLFGLAHVHHGINLYCQEQVPLISAIAASTFQLVYTTAFGILANLFYVRSGYNLWCPIVVHATCNLLGFPSFEMKHTHPRFFYVYCVLLVFGIVAFWKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H790</accession>
    <name>A0A1A0H790_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metallo-dependent phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710293.1">
        <property type="nucleotide sequence ID" value="XM_018857926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H790"/>
    <dbReference type="STRING" id="869754.A0A1A0H790"/>
    <dbReference type="GeneID" id="30030902"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="91"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="450" mass="51177" checksum="2E60B4CAF509048A" modified="2016-10-05" version="1">MPLLPRFRNMAGIMVLLTSLWLVLFVYHEHVVPMLFARRCLWHLVEPEAQTNVLLVADPQLIDNHTYPGRNGVLLGLSKHTVDVYLKQNYRALVTHLAPDYIVFLGDYLDNGRLALDAYFEAEFRRFERIFNRWPEYPRGDRWFTNVAGNHDIGFGDGVKLPLRERFVSHFGAANAVHSINGVDLIVLDSVSYSSSNAAISEEVRHFVRLLPPKQRPRVLLSHVPFYRDSAQLSCGPLRESRELWQNVGYQYQLELTAEVLAELLGLIQPDLIYSGDDHDYCDVQHLDATREVTVKSISMAMGIKRPAVQLLSFSAGGDTLNYHTEICYLPRPYVNVVVYVALAVCSGLLLLFSSIKLRRYVYARGQAPMSKRMSKFFEGDDSPAASLAPLSNYTKTTLSAGEKLQQQFRVWKAEMLLTLRRWNLITFFKLCAVLGLVTIGIYLTVVSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H2</accession>
    <name>A0A1A0H7H2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Amine oxidase</fullName>
            <ecNumber evidence="15">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_33028</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a primary methyl amine + O2 + H2O = an aldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16153"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:228804"/>
            <dbReference type="EC" id="1.4.3.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="15">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="15">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="14">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14 15">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710569.1">
        <property type="nucleotide sequence ID" value="XM_018856197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7H2"/>
    <dbReference type="GeneID" id="30029173"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000014">
        <property type="entry name" value="Peroxisomal primary amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015800">
        <property type="entry name" value="Cu_amine_oxidase_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF86">
        <property type="entry name" value="COPPER AMINE OXIDASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02727">
        <property type="entry name" value="Cu_amine_oxidN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13 15" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N2-terminal" evidence="17">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="18">
        <location>
            <begin position="114"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="16">
        <location>
            <begin position="249"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="13">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="14">
        <location>
            <position position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA20044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="708" mass="79335" checksum="108BB51342DF18AC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7J0</accession>
    <name>A0A1A0H7J0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">quinol--cytochrome-c reductase</fullName>
            <ecNumber evidence="3">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_45564</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="17">Binds 1 heme c group covalently per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710393.1">
        <property type="nucleotide sequence ID" value="XM_018857932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7J0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7J0"/>
    <dbReference type="GeneID" id="30030908"/>
    <dbReference type="OrthoDB" id="5925at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000002">
        <property type="entry name" value="Cytochrome c1, heme protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.100:FF:000003">
        <property type="entry name" value="Cytochrome c1, heme protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021157">
        <property type="entry name" value="Cyt_c1_TM_anchor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266">
        <property type="entry name" value="CYTOCHROME C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10266:SF3">
        <property type="entry name" value="CYTOCHROME C1, HEME PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00603">
        <property type="entry name" value="CYTOCHROMEC1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81496">
        <property type="entry name" value="Cytochrome c1 subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase), transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 17 18" id="KW-0349">Heme</keyword>
    <keyword evidence="13 17 18" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="20">
        <location>
            <begin position="67"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR602326-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA19868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="31455" checksum="8AE2F6D3F777B575" modified="2016-10-05" version="1">MFKSARLSSPLAQKFIASAVGVSGLTASYALYQDSVTASAMTAAEHGLHPPAYGWSHNGMFSTFDHASIRRGYQVYKEVCAACHSMDRIAWRNLVGVSHTASEAKAFAEELEYDDEPDDEGNPRKRPGKLADYIPGPYPNEQAARAANQGALPPDLSLIVKARHGGCDYIFSLLTGYPEEPPAGVVMPPGSNYNPYFPGGAIAMGKVLFDDLVEYEDGTPATASQMAKDVSTFLNWCSEPEHDERKKWGLKAIIVTSSLFLLSVWVKKFKWQPIKNRKFTFNPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7N9</accession>
    <name>A0A1A0H7N9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Phosphoribosylamine--glycine ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_79409</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the second and fifth step in the 'de novo' purine biosynthesis pathway; contains phosphoribosylamine--glycine ligase (GARS) and phosphoribosylformylglycinamidine cyclo-ligase (AIRS) activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the C-terminal section; belongs to the AIR synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710639.1">
        <property type="nucleotide sequence ID" value="XM_018859060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7N9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7N9"/>
    <dbReference type="GeneID" id="30032036"/>
    <dbReference type="OrthoDB" id="2018833at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000006">
        <property type="entry name" value="Phosphoribosylamine--glycine ligase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000006">
        <property type="entry name" value="phosphoribosylamine--glycine ligase, chloroplastic-like"/>
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        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000018">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.600.10:FF:000001">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000007">
        <property type="entry name" value="Trifunctional purine biosynthetic protein adenosine-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="116"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA20114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="793" mass="83928" checksum="5EC2FFB167A99B76" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Y5</accession>
    <name>A0A1A0H7Y5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vps54-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_79394</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710620.1">
        <property type="nucleotide sequence ID" value="XM_018859057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Y5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Y5"/>
    <dbReference type="GeneID" id="30032033"/>
    <dbReference type="OrthoDB" id="10259024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="8">
        <location>
            <begin position="788"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1048"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1048"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1105"/>
            <end position="1114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1114" mass="125901" checksum="5A28F957D44A19BB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Y8</accession>
    <name>A0A1A0H7Y8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF1715-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710736.1">
        <property type="nucleotide sequence ID" value="XM_018858913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Y8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Y8"/>
    <dbReference type="GeneID" id="30031889"/>
    <dbReference type="OrthoDB" id="48036at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052436">
        <property type="entry name" value="LTO1_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532">
        <property type="entry name" value="GEO13458P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28532:SF1">
        <property type="entry name" value="ORAL CANCER OVEREXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="2">
        <location>
            <begin position="20"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="15956" checksum="56CC3DCE21AD2119" modified="2016-10-05" version="1">MTDFSLDDVLNLEDGYYEKGFHEGHEHSAKEQFMEGKVYGLQTGFQRFLIVGYLHGLLEEWSQEKSPALAVHLKQLSTYLAAISMSNDDQAVVQYEKSVTSARNKARVIAAITKTGEKLSALDALIKSVGGNLLVSEDLNEMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8G1</accession>
    <name>A0A1A0H8G1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Terpenoid synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_79454</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710697.1">
        <property type="nucleotide sequence ID" value="XM_018859069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8G1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8G1"/>
    <dbReference type="GeneID" id="30032045"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033386">
        <property type="term" value="P:geranylgeranyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="343" mass="38439" checksum="F2902970C9687AC6" modified="2016-10-05" version="1">MGPPDIERVISLKSLCVPANTLTAPYDYLHLLPSNNDRIRSTFLAGINESFFRISDAAVISEISAVIEIFHGASLLIDDIEDDSAFRRGQPCAHIRYGVPATLNSGNLMYFVAMRRAFQALPPHYTHTKGGLALALVAFRSQMVDVFVEEMVNLHTGQGFDIYWRDNLAQLMKTGMPSIEDYLQVVMHKTGGLFRLAVRTLGLFAGDAFQMHSRRSEGLVELANLLGIIYQIRDDYLNLVDERYAELKGSFGEDLIEGKLSLPILYTLLHSGDNTVLHTLYDELKSPQERRADLDRLAEARCFVADSGALQYTYSLLQEYVEKARSILAAVNKPEAFSKEPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8X2</accession>
    <name>A0A1A0H8X2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710854.1">
        <property type="nucleotide sequence ID" value="XM_018856033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8X2"/>
    <dbReference type="GeneID" id="30029009"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="82" mass="9365" checksum="5BA056F8E30B5810" modified="2016-10-05" version="1">MNNQAHNTFHQINDIYTSQPHTTNRKSTEHCKPKRQPKRQHQLKNSRPSQFQKLPKATKAPRLYGAALSFSLLVILCAAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Z1</accession>
    <name>A0A1A0H8Z1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710998.1">
        <property type="nucleotide sequence ID" value="XM_018854230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Z1"/>
    <dbReference type="GeneID" id="30027206"/>
    <dbReference type="OrthoDB" id="10293127at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1010" mass="112774" checksum="9C8853D45640D3D9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9D6</accession>
    <name>A0A1A0H9D6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Quinone oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43962</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711010.1">
        <property type="nucleotide sequence ID" value="XM_018857726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9D6"/>
    <dbReference type="GeneID" id="30030702"/>
    <dbReference type="OrthoDB" id="809632at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05288">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000121">
        <property type="entry name" value="Prostaglandin reductase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205:SF19">
        <property type="entry name" value="ENOYL REDUCTASE (ER) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PROSTAGLANDIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase N-terminal" evidence="3">
        <location>
            <begin position="27"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="2">
        <location>
            <begin position="172"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="363" mass="39755" checksum="1B343252609608A5" modified="2016-10-05" version="1">MVVPKEARCFVLNEPPVAEVNLDPESANATFKLVKKPLRDLNSGEILVKTLYLSNDPTQRAWIQKGLKPESMYVEPVTQGEIMRLVGLGQVVALKNGKYKEGDILNTTLKWSDYSIISESAIFSKISDTSVPLPMYLDVLGTTGLTAYFGLLQVAKLKSSDVVVISAALGATGSMCVQIAKKVIGCKKVIGISGGREKCHFVELLGADHCVDYHSKSFQKDLSRAIGKDKFCDVFFDGVGGKILDAALGMMRPFGRVVACGAIAGYNDFGQSRVLNWPQIVVKRLNVKGFIVLDFIDQYAEGIRKLHLWIELGLIRYDKSTYSVVDLTGTDADFAKIPASWGELFKPTKGPGKLLTQVSRPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9K7</accession>
    <name>A0A1A0H9K7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_44116</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711085.1">
        <property type="nucleotide sequence ID" value="XM_018857743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9K7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9K7"/>
    <dbReference type="GeneID" id="30030719"/>
    <dbReference type="OrthoDB" id="5329385at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031342">
        <property type="entry name" value="Mug163-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17119">
        <property type="entry name" value="MMU163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="290" mass="32585" checksum="5DF19181FEC18106" modified="2016-10-05" version="1">MNSSSCLRICPLSSHACSHLPRNIERQRNAISQSQDFVIFSLEKAPELKGNAEEQQRILNLGMMTDHLKAAIPTLLQKSLPKALLLQHVLLRICPSFFEQINSYLPNIKGHASYYATCKAAQIFLTSFLLNPHTQLHIELIRTSKFSDSNCLYSRSTKIFVRWSTCSDGCTHLLNPEKAIDTGAVTRSTGTVNFSTANAKLGSHRWSSIDSVQILDHLKQNWTLTGSIVDLAKSIAGVKKDDAKLERIILGIFIFELNEENNQIVVHTIENLNIIERSVEEDVENKLRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9P1</accession>
    <name>A0A1A0H9P1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_32582</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711124.1">
        <property type="nucleotide sequence ID" value="XM_018856090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9P1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9P1"/>
    <dbReference type="GeneID" id="30029066"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="64"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA20602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="457" mass="49830" checksum="81045BF8747CC5EC" modified="2016-10-05" version="1">MVQINEVKDTQGKESRTAAHTHIKGLGLDEHGLAKKIDGGFVGQGEAREACGIIVDLIKTKKMSGKAILLAGAPGTGKTALALAISQELGPKVPFCPIVGSELYSAEVKKTAALMENFRKAIGLRIKETKEVYEGEVIELTPEEAENPLGGYGKTISHVIVGLKTAKGTKSLRLDPSIYESIQKERVVVGDVIYVEANTGAVKRVGRSDAYATEFDLEAEEYVPLPKGEVHKKKEIVQEVTLHDLDVANARPQGGQDVLSMMGQLLKPKKTEITDKLRTEVNKVVSKYIDQGVAELIPGVLFIDEVNMLDIEIFTYLNRALESSMAPIVVLACNRGMTTIRGTDDATKYPHGCPPDLIDRLLIVKTLPYNQDEIRTIISKRATLEGLFVTPEALGRLSHHGVNSSLRYALQLLAPAGILSKTANRSEINITDIEECEVLFLDSSRSMKVLESTKGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W4</accession>
    <name>A0A1A0H9W4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711312.1">
        <property type="nucleotide sequence ID" value="XM_018856121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W4"/>
    <dbReference type="GeneID" id="30029097"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="58" mass="6202" checksum="75D66649C8BE9455" modified="2016-10-05" version="1">MLSSFSQNKCGFGLVASKGSFLGLNKSKEQALIDANNFTMIVQGTAAIWGPEDSHQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Y1</accession>
    <name>A0A1A0H9Y1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenine deaminase</fullName>
            <shortName evidence="7">ADE</shortName>
            <ecNumber evidence="7">3.5.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Adenine aminohydrolase</fullName>
            <shortName evidence="7">AAH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AAH1</name>
        <name evidence="9" type="ORF">METBIDRAFT_31587</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenine + H2O + H(+) = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711449.1">
        <property type="nucleotide sequence ID" value="XM_018855883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Y1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Y1"/>
    <dbReference type="GeneID" id="30028859"/>
    <dbReference type="OrthoDB" id="272271at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009168">
        <property type="term" value="P:purine ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01320">
        <property type="entry name" value="ADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000039">
        <property type="entry name" value="Adenine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01962">
        <property type="entry name" value="Adenine_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028892">
        <property type="entry name" value="ADE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006330">
        <property type="entry name" value="Ado/ade_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01430">
        <property type="entry name" value="aden_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43114">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43114:SF6">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Adenosine deaminase" evidence="8">
        <location>
            <begin position="18"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="39811" checksum="96B5BED7F3F74FDA" modified="2016-10-05" version="1">MASHACTNEFKQFLTDLPKCEHHLHLEGTLEPSLLFALAKRNNITLPELFPATPDACIDRYANFADLQDFLDHYYIGMSVLIKEEDFYDLAMDYFARAHKDGCLHSEVFFDPQGHVERGISIDVVVQGFDRACKAATAKYGTTNKLIMCLLRHLPAADGLQTLDSAALYFENGTIHGMGLDSAEKPFPPELFTECYSTLKFRFPNVGLTAHAGEEGDHTYVSNSLDLLKVTRIDHGVNSCQDEAVMARLTETQTMLSMCPLSNVKLQVVKDVSELPIKVFLDNNISFSINSDDPAYFGGYILDNYIAVHTRFGFTRDQWCQIVANGISGSWCDEQRKAELRANLEAVRTKYAHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA03</accession>
    <name>A0A1A0HA03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial distribution and morphology protein 31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_12660</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711229.1">
        <property type="nucleotide sequence ID" value="XM_018854253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA03"/>
    <dbReference type="GeneID" id="30027229"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900208">
        <property type="term" value="P:regulation of cardiolipin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF0">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="118"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="568"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="588" mass="66467" checksum="69FAD445733900F4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA31</accession>
    <name>A0A1A0HA31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SNF5-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711370.1">
        <property type="nucleotide sequence ID" value="XM_018855872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA31"/>
    <dbReference type="STRING" id="869754.A0A1A0HA31"/>
    <dbReference type="GeneID" id="30028848"/>
    <dbReference type="OrthoDB" id="515064at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="856"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="21"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="173"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="948" mass="105695" checksum="77DBC6DCD86AE673" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA89</accession>
    <name>A0A1A0HA89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ENTH-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_43999</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the epsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711314.1">
        <property type="nucleotide sequence ID" value="XM_018857733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA89"/>
    <dbReference type="STRING" id="869754.A0A1A0HA89"/>
    <dbReference type="GeneID" id="30030709"/>
    <dbReference type="OrthoDB" id="4033880at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16991">
        <property type="entry name" value="ENTH_Ent1_Ent2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000006">
        <property type="entry name" value="Clathrin interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276:SF110">
        <property type="entry name" value="EPSIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12276">
        <property type="entry name" value="EPSIN/ENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="8">
        <location>
            <begin position="10"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="318"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="341"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="480" mass="54232" checksum="E06C265052EE0271" modified="2016-10-05" version="1">MSKIVRSIKNVANGYSATQVLVRNATLNDPLGPTTYDMEQIALASFNSQTEFLEIMDMLDRRLNDKGRNWRHVAKSLTVLDYLVRFGSDKCVLWLKDNLYIVKTLREFVHFDEANNDHGALIRVKAKELVALLRDDERLATERRLAQRSRSQRPQRPPQRNDRPSDPYDADLQRALEISRETAERELHNRPAGPDDTEDPDLQAALKLSLEEEEMRRLRSQLNLQNENLLDLGDTLQFQQQPQYYQPVQYVQPTQFFQPQQTLYQQFDMFGNPIQPTAGGFYQNMTYGQFGEPQPVQSQPQIPQYTGFNVAANPQERLTPLKTGSNNPFALLNDSARQSEQQSQQLAQQAQQQAQQQAQQQAQQQAQVQQQPSRYNETHELNNLLASGTGLDTFGNTGATRIPHQHTKTGTFMNSSGTGYNNAISDHRVSSNATGNPFLNTGIGNTANTANNNTPNPRIDAAHTGYGFGNSKEEPSLIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA98</accession>
    <name>A0A1A0HA98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cofilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Actin-depolymerizing factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_11638</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. Binding to F-actin is regulated by tropomyosin. It is the major component of intranuclear and cytoplasmic actin rods. Required for accumulation of actin at the cell division site via depolymerizing actin at the cell ends. In association with myosin II has a role in the assembly of the contractile ring via severing actin filaments. Involved in the maintenance of the contractile ring once formed. In association with profilin and capping protein, has a role in the mitotic reorganization of the actin cytoskeleton.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711564.1">
        <property type="nucleotide sequence ID" value="XM_018854045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA98"/>
    <dbReference type="STRING" id="869754.A0A1A0HA98"/>
    <dbReference type="GeneID" id="30027021"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.20.10:FF:000060">
        <property type="entry name" value="Cofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="11">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="15830" checksum="4F75985E6E339589" modified="2016-10-05" version="1">MSRSGVAVADESLAAFNDLKLGKKYKFIVFALNAEKTQIVVDETSTDTDYDSFLEKLPENECKYAVYDFEYEIGGGEGKRTKIVFYTWSPDTAPVRSKMVYASSKDALRRALNGIAADIQGTDFSEVAYESVLERVSRGAGSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG1</accession>
    <name>A0A1A0HAG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Uricase</fullName>
            <ecNumber evidence="7 10">1.7.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Urate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_11476</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 10">Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>urate + O2 + H2O = 5-hydroxyisourate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:21368"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="EC" id="1.7.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7 10">Belongs to the uricase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.3.3" evidence="7 10"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711373.1">
        <property type="nucleotide sequence ID" value="XM_018854000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAG1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAG1"/>
    <dbReference type="GeneID" id="30026976"/>
    <dbReference type="OrthoDB" id="9992118at2759"/>
    <dbReference type="UniPathway" id="UPA00394">
        <property type="reaction ID" value="UER00650"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004846">
        <property type="term" value="F:urate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.270.10:FF:000001">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.270.10">
        <property type="entry name" value="Urate Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002042">
        <property type="entry name" value="Uricase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019842">
        <property type="entry name" value="Uricase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03383">
        <property type="entry name" value="urate_oxi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42874:SF1">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01014">
        <property type="entry name" value="Uricase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000241">
        <property type="entry name" value="Urate_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00093">
        <property type="entry name" value="URICASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00366">
        <property type="entry name" value="URICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="4 7 10" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>5-hydroxyisourate</name>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>O2</name>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>urate</name>
            <dbReference type="ChEBI" id="CHEBI:17775"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000241-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="302" mass="34104" checksum="CF6D77C375EF8621" modified="2016-10-05" version="1">MSVLLDSAYGKGNVKFLKVKKDKHNPQVQDVLEANCRVLLKGNFDVSYTKADNSPVVPTDTVKNTILVEAKNTDVWPIERFAAHLAKHFTTKYAHVLGVDIQIVQSRWTKFDVNGKPHDHSFRNEGPETRRTHLEYSKTTGQLTITSAIKDLTVLKSTGSMFYGYHQCDYTILKPTTDRILSTDVDASWKFSSCFKSLDDVFKHADNGVFDQTYEAARKITLDRFALENSPSVQATMYNMSHDILQAAPQIDNVTYELPNNHYVLYDLSWKGITDNKELFYPSPDPNGLICCTVGRNGAPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAM5</accession>
    <name>A0A1A0HAM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_11644</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAPP small subunits family. BET5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711576.1">
        <property type="nucleotide sequence ID" value="XM_018854048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAM5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAM5"/>
    <dbReference type="GeneID" id="30027024"/>
    <dbReference type="OrthoDB" id="3364529at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF16">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="163" mass="17955" checksum="F543F15F4A785ABB" modified="2016-10-05" version="1">MTIHALYIFDRHCSCIYSREYNHQNPGQGTVNKNNDSDMAQLLFGVVYSLKSLSAKLGATESSDNLGSDPGALPPAANTLRSFSTGHYRVHFLESLSNFKFALVTDLQVDNLQAQLWELYSTVFVGHVLRNALAPVEFGTSKLNSAEFTSLSDAYLSSLPVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAT0</accession>
    <name>A0A1A0HAT0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711503.1">
        <property type="nucleotide sequence ID" value="XM_018857369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAT0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAT0"/>
    <dbReference type="GeneID" id="30030345"/>
    <dbReference type="OrthoDB" id="20109at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013241">
        <property type="entry name" value="RNase_P_Pop3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28272:SF1">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08228">
        <property type="entry name" value="RNase_P_pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="262" mass="29301" checksum="FCE166CA112F7753" modified="2016-10-05" version="1">MSKHTPGAGSIRDIEQKKREVLKPLLENPFTQGTEWPTIDRSVAETIMECLIQVLSGYGIYLEMRSLNKDKKIPVPEVAGKITIGFNSTVKKLEKQAGPNRAKLLGKPPKRSKQEQPNTPGYVKYVFVAKSDISTSLLTSCFPLLTYSASRSALDRVKLVELPRGAMQKLSKVLRTDNVSIISLEEKWADGKLLFDVVDSNIRDVEVPWLAALFDENSAPVYEKPNIKFVKTSVPVGRANKKKNGVLKKKKNRKNEGRVLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAT7</accession>
    <name>A0A1A0HAT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ethanolamine kinase</fullName>
            <ecNumber evidence="3">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41961</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711744.1">
        <property type="nucleotide sequence ID" value="XM_018857471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAT7"/>
    <dbReference type="STRING" id="869754.A0A1A0HAT7"/>
    <dbReference type="GeneID" id="30030447"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF66">
        <property type="entry name" value="ETHANOLAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="549" mass="61605" checksum="2043DDFD74080841" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBA0</accession>
    <name>A0A1A0HBA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_178800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711775.1">
        <property type="nucleotide sequence ID" value="XM_018854744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBA0"/>
    <dbReference type="GeneID" id="30027720"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="179" mass="21144" checksum="FBD727002CB35CA5" modified="2016-10-05" version="1">MGREHRSGKWPGKIPGEKTTEWKDTRHVARGSEEIENHDNIIPRLGPRDYRQKHLGANKRIKYRQKKRQSKRRPKYGAKRCDVCRNNKIVVYRDCSARIRCEKIIVRQNCKGGKSQSRTKGQNKKSRQRDQRRKTDESVSAGEQYQDRQGNLGQRHKERESSSIILDDKLRRPSTHRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBB4</accession>
    <name>A0A1A0HBB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_56149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711934.1">
        <property type="nucleotide sequence ID" value="XM_018858299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBB4"/>
    <dbReference type="GeneID" id="30031275"/>
    <dbReference type="OrthoDB" id="4021409at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="38649" checksum="35706A98C683E86B" modified="2016-10-05" version="1" fragment="single">MVSLFDISTQKPFWSHLSTGVPTLDLMLRLQSDALLDLQSVPANSAFSAVLCSLVASHLLLSPELKVVVLETLNAFPWLILEQHPSFDAAWVDQGRLVSYHVPTFAQLYAFFAFMPLQEVEPGSVLVAVTNFHETIEFYRLQISATHEELLLKHQIDKNRCFLEHMPKAMEEGLDAIALPQVPLIARENPYTKAQNHLNGLILMMSDFAFKFSAVVLLAGHLEPKFLPISQIPISQGPTSQNTSFHSKDSSRQVLQPVTLGPSSSEKPNLAESKITARWVFYNDWFDKSPYFARQHKQLGDLHRFFVSVVKVSYLNGVNNINDPVYFHHSKHPMFFDQEDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBG6</accession>
    <name>A0A1A0HBG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711989.1">
        <property type="nucleotide sequence ID" value="XM_018857320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBG6"/>
    <dbReference type="GeneID" id="30030296"/>
    <dbReference type="OrthoDB" id="4093459at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="141" mass="15326" checksum="E4E14E2481CFA0B7" modified="2016-10-05" version="1">MSDWAAKLLQKPSTGTGAGPANSGLASNGKFPLVLQPNQTVKLPAPKTATQETPQKNGRALPEPFNGADVLAWMGTRFAADLEDAQADKTGEYAVVYRSLESSSSWKTTGPGYGRKCTKEDDRYDLLQELNRTLQPKPRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBK2</accession>
    <name>A0A1A0HBK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Farnesyl pyrophosphate synthase</fullName>
            <ecNumber evidence="6">2.5.1.1</ecNumber>
            <ecNumber evidence="5">2.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_42127</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="6"/>
    <dbReference type="EC" id="2.5.1.10" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711875.1">
        <property type="nucleotide sequence ID" value="XM_018857501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBK2"/>
    <dbReference type="STRING" id="869754.A0A1A0HBK2"/>
    <dbReference type="GeneID" id="30030477"/>
    <dbReference type="OrthoDB" id="10257492at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.600.10:FF:000006">
        <property type="entry name" value="Farnesyl pyrophosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="350" mass="40280" checksum="243437AD1E2A8C74" modified="2016-10-05" version="1">MDKAAARQKFVAEFDNLVKELQEISAGYNMPKEAIEWFTKNLEYNTPGGKLNRGLSVIDTYCILKNKTVESLAADEYKKVALLGWAIELLQAYFLVADDMMDQSKTRRGQPCWYLAEGVGNIAINDSFMLEGAIYILLKKHFRQDLYYVDLLDLFHEVTFQTELGQLLDLVTADEEHVDLSKFSLEKHSFIVIFKTAYYSFYLPVALAMYMCGINSDEDLKQVKDVLIPLGEYFQIQDDYLDCFGTPEQIGKIGTDIKDNKCSWVVNQALLKANESQRQVLDDNYGKKDDESEQRVKDLFAELQIPSIYEQYEEEVVAKLRAQIEKIDESRGLKKEVLSSFLAKVYKRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBN3</accession>
    <name>A0A1A0HBN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_179508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711797.1">
        <property type="nucleotide sequence ID" value="XM_018854754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBN3"/>
    <dbReference type="GeneID" id="30027730"/>
    <dbReference type="OrthoDB" id="4085791at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="480" mass="53345" checksum="980BACB3B67C0E7F" modified="2016-10-05" version="1">MATKPDIATNFASQNLLSENKKKYGDLKRQQKTISSLPRTLRVNRRNKYSVVNQNSTSAKYKTGFVPVKSPGSKLLSFSAPVLLSSPKRVRRRRRQASRQSSLSERTFRKDPNGTRSKIRAIPIAVGGALIKKSTCSLRKVMAEKIQKTTEVWLPSLKMSNLTHWERPFSRPLNAIEQVLNDEIVEPTSSYLCGFRQFLNCKLESTFAGKSPTNSRSIQNAETPQTLGEVVVEGTNQGQPRGPTHKIDHQEETDGDKKRGRSKFRRLAQVGTYAKLFVQKLKIKSLIGRGGSDDNFAEIDVPNIPSPSSDTLKALRRSKAPMVLLSACLSFLFGLPIRPYPPAKRGPQMIMTPILTSTYNNLLLSPGKEIYLADTADSTDLRSERMKQILRVRFSMNNGQVVGDGRTVYRKPSHGLADLTSGGIPLTPMSCILEQDPDFESFMLPLDSGYTLQSPGSNEVDHGYIPKSSGTFSEFGYQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI2</accession>
    <name>A0A1A0HCI2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Clavaminate synthase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_30841</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712320.1">
        <property type="nucleotide sequence ID" value="XM_018855724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCI2"/>
    <dbReference type="GeneID" id="30028700"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050910">
        <property type="entry name" value="JMJD6_ArgDemeth/LysHydrox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480">
        <property type="entry name" value="ARGININE DEMETHYLASE AND LYSYL-HYDROXYLASE JMJD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12480:SF21">
        <property type="entry name" value="JMJC DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="273"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="567" mass="64545" checksum="9F600DC15A9A928D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCJ2</accession>
    <name>A0A1A0HCJ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_78586</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712211.1">
        <property type="nucleotide sequence ID" value="XM_018858903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCJ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCJ2"/>
    <dbReference type="GeneID" id="30031879"/>
    <dbReference type="OrthoDB" id="2942533at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000374">
        <property type="entry name" value="Mitochondrial proteins import receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="389"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="603" mass="66279" checksum="34CE75047B450668" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCY2</accession>
    <name>A0A1A0HCY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-aminobutyrate aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_39522</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712450.1">
        <property type="nucleotide sequence ID" value="XM_018857150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCY2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCY2"/>
    <dbReference type="GeneID" id="30030126"/>
    <dbReference type="OrthoDB" id="10260828at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000073">
        <property type="entry name" value="Probable 4-aminobutyrate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="470" mass="51496" checksum="530FD5D37B295554" modified="2016-10-05" version="1">MSVCEKYFPEEPAGPHTASAVPGPQSQRAIQSLGQVFDSRPAYFVADYTRSVGNYIADVDGNLYLDVYAQIASIPLGYNNPALVAAAKSDRMVSAIVNRPALGNFPGSDTEHIIRDLLKVAPQGQDKMWSGLSGADANELAFKAAFMWYQLKRRGAGAAFSAHETASVMKNQAPGAPDLAILSFQRAFHGRLFASASATCSKPIHKMDLPAFNWPKAEFPAYKYPLEAHRDANSREDARCLAIVDTLLKSWNVPIAAVLVEPIQSEGGDNHASAAFFQGLRDLTLAHGALLIVDEVQTGVGATGKMWAHEHFDLEPKPDMVTFLKKFQSAGYYYHDPALTPAQPYRQFNTWCGDPARMILAGAIGHEIVEHDLAKQAARVGDYLYGKLAKMADKYPDYLADLRGKDRATFIAWTFKTPQLRDRFLADMKTVGINIGGCADDSVRLRPTLVFEEKHADVLVAGIEKVLLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD21</accession>
    <name>A0A1A0HD21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cell division control protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30908</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712407.1">
        <property type="nucleotide sequence ID" value="XM_018855736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD21"/>
    <dbReference type="STRING" id="869754.A0A1A0HD21"/>
    <dbReference type="GeneID" id="30028712"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032160">
        <property type="term" value="C:septin filament array"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031107">
        <property type="term" value="P:septin ring disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000162">
        <property type="entry name" value="septin-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="9">
        <location>
            <begin position="32"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="393"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="417" mass="47835" checksum="75E5E67872A21BEC" modified="2016-10-05" version="1">MDQELKIIRRKVNGFVGFANLPKQWHRKSVRRGFTFNCMVIGESGMGKATLINTLFNREVLSSRVDDAKSVDNEGDEPTLRIENTTSEIEEDGVKLKLNIITAPGFGEHVNNTDSWKPIVDEINTRFDHYLEAESRINRSAITDNRIHALLYFIEPTGHSLKSLDIQLMKLVHEKVNLIPIIAKSDTLIDDEIATFKARVLADINYQKIKIFTPQVYENDDEDITGNSRALIASFPFAVIGSTTDVQTADGRSVRGRAYPWGIIEVDNENHNDFVRLRQLLVRNNLEELRETTSDTLYEKYRSEKLEKMGIEQDNSVFKEFDPLTKQEEERALHEAKLAKMEAEMKAVFQQKVSEKEKKLQRSEADLFARHKEMKEKLAKQIKLLEDKKAQLEKQKALPVEHAPAPAAQKSRKGFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD72</accession>
    <name>A0A1A0HD72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="9">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_38925</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712525.1">
        <property type="nucleotide sequence ID" value="XM_018857068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD72"/>
    <dbReference type="STRING" id="869754.A0A1A0HD72"/>
    <dbReference type="GeneID" id="30030044"/>
    <dbReference type="OrthoDB" id="272392at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055207">
        <property type="entry name" value="POLR3C_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008806">
        <property type="entry name" value="RNA_pol_III_Rpc82_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05645">
        <property type="entry name" value="RNA_pol_Rpc82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20912">
        <property type="entry name" value="RPC3_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22536">
        <property type="entry name" value="WHD_POLR3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="11">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III Rpc82 C -terminal" evidence="10">
        <location>
            <begin position="147"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase III subunit RPC3 winged-helix" evidence="12">
        <location>
            <begin position="429"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="589" mass="65249" checksum="E53A84E553E39048" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD74</accession>
    <name>A0A1A0HD74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cysteine proteinase 1, mitochondrial</fullName>
            <ecNumber evidence="2 9">3.4.22.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_40171</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity.</text>
    </comment>
    <comment type="function">
        <text evidence="7">The normal physiological role of the enzyme is unknown, but it is not essential for the viability of yeast cells. Has aminopeptidase activity, shortening substrate peptides sequentially by 1 amino acid. Has bleomycin hydrolase activity, which can protect the cell from the toxic effects of bleomycin. Has homocysteine-thiolactonase activity, protecting the cell against homocysteine toxicity. Acts as a repressor in the GAL4 regulatory system, but this does not require either the peptidase or nucleic acid-binding activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred.</text>
            <dbReference type="EC" id="3.4.22.40"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. Binds to nucleic acids. Binds single-stranded DNA and RNA with higher affinity than double-stranded DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase C1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.40" evidence="2 9"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712462.1">
        <property type="nucleotide sequence ID" value="XM_018857240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD74"/>
    <dbReference type="GeneID" id="30030216"/>
    <dbReference type="OrthoDB" id="2666448at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070005">
        <property type="term" value="F:cysteine-type aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043418">
        <property type="term" value="P:homocysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00585">
        <property type="entry name" value="Peptidase_C1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025660">
        <property type="entry name" value="Pept_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004134">
        <property type="entry name" value="Peptidase_C1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10363:SF2">
        <property type="entry name" value="BLEOMYCIN HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03051">
        <property type="entry name" value="Peptidase_C1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005700">
        <property type="entry name" value="PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00639">
        <property type="entry name" value="THIOL_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="10">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005700-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="471" mass="52698" checksum="E0142185E3ECDDB4" modified="2016-10-05" version="1">MAPLETLVEGLTIGSSDSSSAITAGNLAAWETQLLQDPKNRLAQNALAKNAITDIVAASGSELSLKNRYFFNVEVDTIGSPSFLNNQKSSGRCWIFATCNVLRAHVIKKYGLQPDSFQLSQSYLFFYDKLEKANYFLENIIDTASEPVDSRLLQFLFSGSVSDGGQWDMIVNVIEKYGVVPNEVFPDNSQASSTSQLNYLLTNKLREYGLVLRKLVHQGTPRNEVLAVKNAMNKEVYNIIALALGTPPKPGDRFKWEFLDKSGKYLAYDTTPLEFYQSHTGYDVSKRFSLIHDPRNPYNSLYTVDRLNNVSGGKPIEYVNLELPAIKKAAVAMLKSNEPIFFGSDVGKFNNNTTGVLDTGAYDYKLAFNTSLKLSKLERLQTGSSAMTHAMVITGVHLDNVSGAPVRWKIENSWGDQVGDKGYFVMTDAWFDEYVFQIVTSKKYVEKSVYDLWKAKKYEQLPFYDPMGALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDC4</accession>
    <name>A0A1A0HDC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_39056</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712393.1">
        <property type="nucleotide sequence ID" value="XM_018857087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDC4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDC4"/>
    <dbReference type="GeneID" id="30030063"/>
    <dbReference type="OrthoDB" id="1108038at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16702">
        <property type="entry name" value="RING_CH-C4HC3_MARCH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="561"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="626"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="658"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="833"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="995"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1026"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1060"/>
            <end position="1078"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="278"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1110" mass="123226" checksum="DF7381766AE9AFBA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDD4</accession>
    <name>A0A1A0HDD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_40318</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712481.1">
        <property type="nucleotide sequence ID" value="XM_018857258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDD4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDD4"/>
    <dbReference type="GeneID" id="30030234"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17998">
        <property type="entry name" value="DEXHc_SMARCAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000014">
        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="507"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="872"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="95"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="451"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="762"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="762"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1042" mass="118077" checksum="6789BFAFA6A93FF2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDE6</accession>
    <name>A0A1A0HDE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_10993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712600.1">
        <property type="nucleotide sequence ID" value="XM_018853897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDE6"/>
    <dbReference type="GeneID" id="30026873"/>
    <dbReference type="OrthoDB" id="4018477at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="30367" checksum="0A4C8AC4393AC525" modified="2016-10-05" version="1">MPVSSTPSKRVALAPINVNVASPLHTLKTRHPTTAPGLPLTPGLTPKLGLVKSMTSLAPAGPRASALSGAARARKMDSFSEYKITNSKNVKADLAATKLKLRLQLAVYKLRLGRDAAMAPAPTFRVSKPLRLLTLLRLLPVFSESANVNLQRPQPAPRAAPAEPAAAASMKLYHIKPLSAFYNAYSHQLPLTSTHLQRLPPVNKILRTPIKAASRALLQMGAAHAASNSDETIDETVHESGGGLLKRKADILGSSPLRHNSFGNGIGTPNSFSVAKSLLQLGLGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDF8</accession>
    <name>A0A1A0HDF8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative translation elongation factor 1-beta (EF-1-beta)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77526</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712506.1">
        <property type="nucleotide sequence ID" value="XM_018858717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDF8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDF8"/>
    <dbReference type="GeneID" id="30031693"/>
    <dbReference type="OrthoDB" id="331763at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.60:FF:000001">
        <property type="entry name" value="Elongation factor 1-beta 1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053836">
        <property type="entry name" value="Arc1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018940">
        <property type="entry name" value="EF-1_beta_acid_region_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049720">
        <property type="entry name" value="EF1B_bsu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001326">
        <property type="entry name" value="Transl_elong_EF1B_B/D_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595">
        <property type="entry name" value="EF-HAND AND COILED-COIL DOMAIN-CONTAINING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11595:SF21">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21972">
        <property type="entry name" value="Arc1p_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10587">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01182">
        <property type="entry name" value="EF-1_beta_acid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00824">
        <property type="entry name" value="EF1BD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00825">
        <property type="entry name" value="EF1BD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="6">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="5">
        <location>
            <begin position="124"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="210" mass="22961" checksum="26E89E39D1F24893" modified="2016-10-05" version="1">MSFSDFSKVETLKSLNEFLADKSYVDGTAATSADVSVYQAFQKTYPAFARWFNHIASFTEEFETLPAGKAPAGSAAAAEEDDDDVDLFGSDDEEVDEAAEKLKQERLAEYAAKKASKGPKPAAKSIVTMDVKPWDDETDLEQLLANVKAIEMEGLTWGASLWIPVGFGIKKLQINLVVEDALVSLDELQAAIEEDEDHVQSTDIAAMQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDL9</accession>
    <name>A0A1A0HDL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">A to I editase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_40144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712488.1">
        <property type="nucleotide sequence ID" value="XM_018857237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDL9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDL9"/>
    <dbReference type="GeneID" id="30030213"/>
    <dbReference type="OrthoDB" id="10268011at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043829">
        <property type="term" value="F:tRNA-specific adenosine-37 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002466">
        <property type="entry name" value="A_deamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042935">
        <property type="entry name" value="Tad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47803:SF1">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02137">
        <property type="entry name" value="A_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00552">
        <property type="entry name" value="ADEAMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50141">
        <property type="entry name" value="A_DEAMIN_EDITASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A to I editase" evidence="2">
        <location>
            <begin position="56"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="383" mass="41261" checksum="4D5DF2EC87EE76EF" modified="2016-10-05" version="1">MSRDHLGQRIADAVEAHFEKASGKGGRPGVRPGGVAEWTVLAGLVAVDGDSLTVLSAATGVKAMPDCVRRYSRGLIVHDMHAEILCLRMFNWYVVEEVKHAARRRFLEPTGAAGPGGPRFRWRPGVRLALYVSEPPCGDASMAHDEAGKEATEKRSTEKRSRTTPAAPATDGPAAKKHSAVRGRAGFGDLGVVRTKPGRADSRVSFSKSCSDKLCAKQFTGILNCVASVRVDPVFLDFLVVAEQKHSPADFRRCFVSRLGPLPPPPPHALQVLTFGASRYAYAKHAGRVPLPLSWVSCPVAGTAHVLSQGVRNGAHVKNGPPRRAGASTLCRRSLWEHAQGEVPVHGSYRQFKQAAVGRERRKHEARAALGAWPACAEDDFAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW7</accession>
    <name>A0A1A0HDW7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712588.1">
        <property type="nucleotide sequence ID" value="XM_018855761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW7"/>
    <dbReference type="GeneID" id="30028737"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291800" evidence="1">
        <location>
            <begin position="19"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="144" mass="16219" checksum="2098BE5596699BDE" modified="2016-10-05" version="1">MQLISKISFLSAVSVTLAFPQMSRFIECLGEDSNRYIVDNPCDSVVAIYSKEFRDFDTKGPDKQLDKCLNYVALIDSETSYMIAFPEADAFSGYVLDSPIGFDHFQTHPEFSGAFCSEKSTNKADQFKSTFTQKSEEAKMLDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDX4</accession>
    <name>A0A1A0HDX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_26273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712795.1">
        <property type="nucleotide sequence ID" value="XM_018855314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDX4"/>
    <dbReference type="GeneID" id="30028290"/>
    <dbReference type="OrthoDB" id="10008801at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="9743" checksum="0C995660DB786A2C" modified="2016-10-05" version="1" fragment="single">SDDEASVRDLISKLNENPEIKEILALFQLLLVSKGLNPEKPPSMMEIMRLFADKEVREMAGKLKAKFDEAGIQISPDQMNLFMGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEQ1</accession>
    <name>A0A1A0HEQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_152459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712863.1">
        <property type="nucleotide sequence ID" value="XM_018854577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEQ1"/>
    <dbReference type="GeneID" id="30027553"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8029" checksum="D8E696B16D7B3871" modified="2016-10-05" version="1">MCWISLGMCLVVFGNVFGYLWECICVLWAGRPRWILGPAIIGGPGALGLVWQFVFRFLAVFFPVFGNVLSCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HER7</accession>
    <name>A0A1A0HER7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Fumarylacetoacetase-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_86981</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712899.1">
        <property type="nucleotide sequence ID" value="XM_018859272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HER7"/>
    <dbReference type="STRING" id="869754.A0A1A0HER7"/>
    <dbReference type="GeneID" id="30032247"/>
    <dbReference type="OrthoDB" id="74910at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018773">
        <property type="term" value="F:acetylpyruvate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820">
        <property type="entry name" value="ACYLPYRUVASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11820:SF7">
        <property type="entry name" value="ACYLPYRUVASE FAHD1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="17"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="176" mass="19624" checksum="99F204441E885DF4" modified="2016-10-05" version="1">MLLTKELNSCTRQQLFFFFLKPSSSILKPGSGPVSVPFGTIVHHDVELAVILGKDLRNQPKDFSPEDALEEIDGYALSIDTIAWNGLPWLIGKGFDTFLPWSEFIPKENIPDPYNVRLYHKVNEETKQDDIASLMIFAIHKILKKGDLIFTGTSKGVGPVKPRDHITASSGDPKNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF03</accession>
    <name>A0A1A0HF03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="12">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_27378</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713077.1">
        <property type="nucleotide sequence ID" value="XM_018855335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF03"/>
    <dbReference type="STRING" id="869754.A0A1A0HF03"/>
    <dbReference type="GeneID" id="30028311"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044547">
        <property type="term" value="F:DNA topoisomerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007534">
        <property type="term" value="P:gene conversion at mating-type locus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044818">
        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010947">
        <property type="term" value="P:negative regulation of meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010520">
        <property type="term" value="P:regulation of reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031860">
        <property type="term" value="P:telomeric 3' overhang formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000296">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000340">
        <property type="entry name" value="Bloom syndrome, RecQ helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022758">
        <property type="entry name" value="Helicase_Sgs1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11408">
        <property type="entry name" value="Helicase_Sgs1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="397"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="596"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="HRDC" evidence="13">
        <location>
            <begin position="955"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA22581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1033" mass="116728" checksum="41C7FC35BA63A0CA" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF44</accession>
    <name>A0A1A0HF44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_87063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the VAM6/VPS39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713013.1">
        <property type="nucleotide sequence ID" value="XM_018859274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF44"/>
    <dbReference type="STRING" id="869754.A0A1A0HF44"/>
    <dbReference type="GeneID" id="30032249"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019452">
        <property type="entry name" value="VPS39/TGF_beta_rcpt-assoc_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF49">
        <property type="entry name" value="VAM6_VPS39-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10366">
        <property type="entry name" value="Vps39_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="17"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="517"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="523"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1045" mass="117888" checksum="92860EF6B28C31B5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF49</accession>
    <name>A0A1A0HF49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endonuclease III homolog</fullName>
            <ecNumber evidence="8">3.2.2.-</ecNumber>
            <ecNumber evidence="8">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="8">DNA glycosylase/AP lyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NTG1</name>
        <name evidence="10" type="ORF">METBIDRAFT_62339</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="8"/>
    <dbReference type="EC" id="4.2.99.18" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713240.1">
        <property type="nucleotide sequence ID" value="XM_018858323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF49"/>
    <dbReference type="STRING" id="869754.A0A1A0HF49"/>
    <dbReference type="GeneID" id="30031299"/>
    <dbReference type="OrthoDB" id="2099276at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03183">
        <property type="entry name" value="Endonuclease_III_Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030841">
        <property type="entry name" value="NTH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="9">
        <location>
            <begin position="161"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="41987" checksum="40524B917CA4CC5B" modified="2016-10-05" version="1">MSNDAMASKLRPLLKMPPKRLAECLLDSPVKTRRSARLWLLADTYSHSETVSPAKIEVKQELTEAKVKVEYEENVLSGGAVSTFYSQIGSNIFQPVSPKHVTAKQPRDWDLIYNEIVKMRALISTPVDSFGCERLPNSITPGMSATDPKTYRFQLLISLMLSAQTKDETNFAAMKTLQEHFVGKGHPGICLDAVISSTEAEIDMCIAKVGFHRRKALYIKKSCDILKERFGGEIPKTIEEIVTLPGVGPKMGHLILQNGWSINSGIGVDVHLHRLAQMWGWVPKSEKPELTRTTLEDWLPKQYWADINPLLVGFGQTVCAPKANNCDVCSLANGLCKGVNRKLVNAKLTEARLKKLSKQRGDLSGLVKRKFEQDAGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF99</accession>
    <name>A0A1A0HF99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">tRNA-splicing endonuclease subunit Sen34</fullName>
            <ecNumber evidence="2 8">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_89790</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="2 8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713152.1">
        <property type="nucleotide sequence ID" value="XM_018859299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF99"/>
    <dbReference type="STRING" id="869754.A0A1A0HF99"/>
    <dbReference type="GeneID" id="30032274"/>
    <dbReference type="OrthoDB" id="48041at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000008">
        <property type="entry name" value="tRNA-splicing endonuclease subunit Sen34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016690">
        <property type="entry name" value="TSEN34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059049">
        <property type="entry name" value="TSEN34_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26577">
        <property type="entry name" value="TSEN34_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017250">
        <property type="entry name" value="tRNA_splic_SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TSEN34 N-terminal" evidence="11">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="10">
        <location>
            <begin position="138"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR017250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR017250-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF26577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="243" mass="27219" checksum="DD3C3078C15AEA7D" modified="2016-10-05" version="1">MVPLLVTGDSILVFDVEHVRVLREHGIVGVLIGSLPKAPQQNVFLGLPLQLSIYEAIWLIQAGFAILLDGAKYSEFLAGTNSLSPAVVSLLNSVQYAVTLDTHKPEADADLGPYTILPQSMIEKYCTPKSEFQKPAEFWHFYFAFKYLRELQYFMMPGLRFGGIFVAYPGDPLKFHSHLIVKALKPEEEIDLLKIVTSGRLATAVKKSWLLVAEKKRNNEDEKPSMKDSPEMLAFSIEWAGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFE0</accession>
    <name>A0A1A0HFE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9086</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713197.1">
        <property type="nucleotide sequence ID" value="XM_018859314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFE0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFE0"/>
    <dbReference type="GeneID" id="30032289"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03754">
        <property type="entry name" value="proteasome_alpha_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000026">
        <property type="entry name" value="Proteasome subunit alpha type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034642">
        <property type="entry name" value="Proteasome_subunit_alpha6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="247" mass="27141" checksum="B8AA7A6DB41C5B7B" modified="2016-10-05" version="1">MSGSAGFDRHITIFSPEGRLFQVEYAFKAINSANITSIGVTGTDSAVLISQKKIPDKLLDPATVLYLFKITPSIGMLATGSIADARSLALRARSEAAEFRYKYGYEMPVQSIAKRMANVSQIYTQRAYMRPLGVALTFCQVDFEDEARGPQLFKCDPAGYYTGVKAVATGPKQQEATTHLEKKFKKTECVNGDWKATVEFAITALSSVLGTDFRKNDIEIGVATQGEFRVLTPDEIDERLVAIAELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ0</accession>
    <name>A0A1A0HFQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_75889</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713164.1">
        <property type="nucleotide sequence ID" value="XM_018858437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFQ0"/>
    <dbReference type="GeneID" id="30031413"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035974">
        <property type="term" value="C:meiotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120105">
        <property type="term" value="C:mitotic actomyosin contractile ring, intermediate layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034973">
        <property type="term" value="C:Sid2-Mob1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044878">
        <property type="term" value="P:mitotic cytokinesis checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903501">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903473">
        <property type="term" value="P:positive regulation of mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902846">
        <property type="term" value="P:positive regulation of mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902854">
        <property type="term" value="P:positive regulation of nuclear migration during mitotic telophase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905758">
        <property type="term" value="P:positive regulation of primary cell septum biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031028">
        <property type="term" value="P:septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05600">
        <property type="entry name" value="STKc_Sid2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000319">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000109">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="186"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="12">
        <location>
            <begin position="487"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="603" mass="69620" checksum="BFE75FA25C33E2BE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFR6</accession>
    <name>A0A1A0HFR6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_35532</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713480.1">
        <property type="nucleotide sequence ID" value="XM_018856621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFR6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFR6"/>
    <dbReference type="GeneID" id="30029597"/>
    <dbReference type="OrthoDB" id="10023235at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031332">
        <property type="term" value="C:RNAi effector complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000018">
        <property type="entry name" value="Serine/threonine-protein kinase 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016685">
        <property type="entry name" value="Silence_cplx_Nase-comp_TudorSN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF2">
        <property type="entry name" value="STAPHYLOCOCCAL NUCLEASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017179">
        <property type="entry name" value="RISC-Tudor-SN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TNase-like" evidence="6">
        <location>
            <begin position="154"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="5">
        <location>
            <begin position="718"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR017179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="904" mass="98655" checksum="7653FA22B54EF599" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGA1</accession>
    <name>A0A1A0HGA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF1690-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_9254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713388.1">
        <property type="nucleotide sequence ID" value="XM_018859344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGA1"/>
    <dbReference type="GeneID" id="30032319"/>
    <dbReference type="OrthoDB" id="5544375at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044284">
        <property type="term" value="C:mitochondrial crista junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012471">
        <property type="entry name" value="DUF1690"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07956">
        <property type="entry name" value="DUF1690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="148" mass="16318" checksum="C6723F8339ACFCF5" modified="2016-10-05" version="1">MGSSASKPETKVFSPKAPVDYSASFLSHLENSQESDYTRAQFTESYIQERVAAELQKLELEAITKYQASASDALLKDGSDGASYSASNDKLLALSKTLQDNASLIKIELDEPVVIARQEVISCLKTNKGKALNCWDEVEQFKKLVHNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGF6</accession>
    <name>A0A1A0HGF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Myoadenylate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_37872</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.6" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713730.1">
        <property type="nucleotide sequence ID" value="XM_018856924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGF6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGF6"/>
    <dbReference type="GeneID" id="30029900"/>
    <dbReference type="OrthoDB" id="1723809at2759"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00663"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003876">
        <property type="term" value="F:AMP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046033">
        <property type="term" value="P:AMP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006178">
        <property type="term" value="P:guanine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01319">
        <property type="entry name" value="AMPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.800.20:FF:000001">
        <property type="entry name" value="AMP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000035">
        <property type="entry name" value="Probable amp deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006650">
        <property type="entry name" value="A/AMP_deam_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006329">
        <property type="entry name" value="AMPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01429">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11359:SF0">
        <property type="entry name" value="AMP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19326">
        <property type="entry name" value="AMP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001251">
        <property type="entry name" value="AMP_deaminase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00485">
        <property type="entry name" value="A_DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="737" mass="86320" checksum="94F63BFDB4892293" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGH9</accession>
    <name>A0A1A0HGH9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF1000-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_103264</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713755.1">
        <property type="nucleotide sequence ID" value="XM_018853739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGH9"/>
    <dbReference type="GeneID" id="30026715"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="3">
        <location>
            <begin position="25"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="237" mass="26956" checksum="7DDC930719BD1AAC" modified="2016-10-05" version="1">MSCENEHFHSHGSHGDDGHDHDDHVAPEETYSSQLLNLKINLFQVTALNLANPPEELPKLFKPAPLRFQLKPVIKSDADEQLIVHIPFLNGSVKLHSVILRTNGDKYCPRTIKLFKNNRNIDFDNVNDTKATFTLTHPHVGVSLNDDGEDEEMPEALESTADFVEHHVPRHVFTGVHTLTMFVENIHGDEEECHFHSVELRGEYTELSRDPVITIYELAANPADHKRVEETLSLMNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGJ0</accession>
    <name>A0A1A0HGJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_29642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713591.1">
        <property type="nucleotide sequence ID" value="XM_018855452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGJ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGJ0"/>
    <dbReference type="GeneID" id="30028428"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF25">
        <property type="entry name" value="DUAL SPECIFICITY PHOSPHATASE CATALYTIC DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_1G03540)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="558"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="635" mass="71076" checksum="08A399407C8A5D80" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGJ2</accession>
    <name>A0A1A0HGJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_76203</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713602.1">
        <property type="nucleotide sequence ID" value="XM_018858492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGJ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGJ2"/>
    <dbReference type="GeneID" id="30031468"/>
    <dbReference type="OrthoDB" id="2530521at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000059">
        <property type="term" value="P:negative regulation of ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000749">
        <property type="term" value="P:positive regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000026">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF4">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="WW" evidence="6">
        <location>
            <begin position="3"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="7">
        <location>
            <begin position="62"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="19285" checksum="EC9E77EF92FE4CAE" modified="2016-10-05" version="1">MDTGLPASWSIRLSKTHNEPYFLNLATRELSWEPPYGSDVDKVSSYIAKYKENGNKPVVAADGKVRALHLLVKNDTSRRPKSWKSPEGVTRTRDEAIAMLRGFQKRILNGEISLADLARSESDCSSHEKGGDLGFFAKGQMQPPFEEAAFALNVGEYSDIIETDSGVHLIQRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGN4</accession>
    <name>A0A1A0HGN4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_76136</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713494.1">
        <property type="nucleotide sequence ID" value="XM_018858481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGN4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGN4"/>
    <dbReference type="GeneID" id="30031457"/>
    <dbReference type="OrthoDB" id="1405469at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF190">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE MIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="170"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="688" mass="77848" checksum="93F75E8F71B30DFC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGR2</accession>
    <name>A0A1A0HGR2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37339</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713845.1">
        <property type="nucleotide sequence ID" value="XM_018856860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGR2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGR2"/>
    <dbReference type="GeneID" id="30029836"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF561">
        <property type="entry name" value="PROTEIN TYROSINE PHOSPHATASE 36E, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="6">
        <location>
            <begin position="52"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="4">
        <location>
            <begin position="328"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="5">
        <location>
            <begin position="475"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="588" mass="67203" checksum="6162EB1C15CD4BE0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGW2</accession>
    <name>A0A1A0HGW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hydantoinase B multi-domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_76184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxoprolinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713569.1">
        <property type="nucleotide sequence ID" value="XM_018858487.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HGW2"/>
    <dbReference type="GeneID" id="30031463"/>
    <dbReference type="OrthoDB" id="3643at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049517">
        <property type="entry name" value="ACX-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365:SF2">
        <property type="entry name" value="5-OXOPROLINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="5-OXOPROLINASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19278">
        <property type="entry name" value="Hydant_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase/oxoprolinase N-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="2">
        <location>
            <begin position="238"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Acetophenone carboxylase-like C-terminal" evidence="5">
        <location>
            <begin position="555"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Hydantoinase B/oxoprolinase" evidence="3">
        <location>
            <begin position="752"/>
            <end position="1285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1318" mass="141482" checksum="255E4425236C84E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHA1</accession>
    <name>A0A1A0HHA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sin3 binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_35355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714040.1">
        <property type="nucleotide sequence ID" value="XM_018856602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHA1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHA1"/>
    <dbReference type="GeneID" id="30029578"/>
    <dbReference type="OrthoDB" id="5391991at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000432">
        <property type="term" value="P:positive regulation of transcription from RNA polymerase II promoter by glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018818">
        <property type="entry name" value="Stb3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28164:SF1">
        <property type="entry name" value="PROTEIN STB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10330">
        <property type="entry name" value="Stb3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="49865" checksum="3BADE88251320769" modified="2016-10-05" version="1">MTSLPPNVSQLLSPSPETQAQPEPGSSEKKHITKLSTSSPEAIRVASAITPNRLANLLMRNGPLAIRLITAQLSIEVPGFDLLSLSKQRRLIMAAMEQGDPQNNVVFEKIGWGQWAVRHVDSDYIVTEPNGALDGSARLNIHELRETAKLGWLKKIEPARRDSISNNKSNMHNFRLPGEKLESPSTGMSDSEQDSDDDEDMGQPSSGSDELTNMRNLKMHDENGSAMAKSKKSPPLKFANRVPTKFSPPPALSGGRRKLSSGTAPNSVVKNTNLHGVNRHQLFNRSRLNSIENLDEYILSSARNSSLSVLSPTVHNSSLPSSSPVGSWNGKGELLHKQELPLPGDHITTALQNAGRRKLSFNESSIRSTLSTSLPRGHALSQKPYENGNSSILSTFSSTGPRKAMAVQDMASDTDEEDWATLGAETLRSHPVKTSNPTGGKLQNEERSAARALVDLMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHE6</accession>
    <name>A0A1A0HHE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713778.1">
        <property type="nucleotide sequence ID" value="XM_018856511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHE6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHE6"/>
    <dbReference type="GeneID" id="30029487"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001278">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="316" mass="33700" checksum="64F6A6A6CDC51626" modified="2016-10-05" version="1">MSRLLLSAIPRQTASALFLGPIRPAARHLHSSWARTHENPLGLPKTGADGPGIPRMSRGLPVRRRIANVGKVLLVSSAKGGVGKSTVLVNVALALRKLGKRCGVLDVDIFGPSIPKLLRLQGEPRLSSSGKLIPLSNYGLPSMSMGYLVAPESPVVWRGLMVMKALQQLLFEVEWPALDYLVIDMPPGTGDTQLTISQLVKVDGALIVSTPQDIALIDAVKGIAMFEKVSIPILGLVQNMSHFVCPNCHHEAHIFGLDGARRAALEHGLDVLGSIPLHETICVQSDLGVPVVVAGGDPLILEPYIGIGRKVVEKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHJ1</accession>
    <name>A0A1A0HHJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_38605</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713794.1">
        <property type="nucleotide sequence ID" value="XM_018857023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHJ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHJ1"/>
    <dbReference type="GeneID" id="30029999"/>
    <dbReference type="OrthoDB" id="244190at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd15859">
        <property type="entry name" value="SNARE_SYN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="3">
        <location>
            <begin position="185"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="275" mass="31110" checksum="004E47F140C62D4B" modified="2016-10-05" version="1">MSVSTKSIKTQLQKVNIYIEQLGDLLDERERLVSVLHLTPSNLDNLDVINLLAKIKAGLDYAQEDLSSGISKDLSAELLKVAKAYSEAAAPLQDDVYINIEEYLFEPLRNDEQSTKKSVRFKDYDSEEEVDETTQMRNQLMGTSGNFKPYKDSTGDPESEDSVDADRNTLLSVDTSNQEMFAQHQQQMLEQDQNLDVLHQSIRTQHRMGAHINEELDEHLILLNDLEEGVDGSHTRIRRATNGIVKFRRKAKENGSLVTIVVLTVILILLLVVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHZ6</accession>
    <name>A0A1A0HHZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_38622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714265.1">
        <property type="nucleotide sequence ID" value="XM_018857025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHZ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHZ6"/>
    <dbReference type="GeneID" id="30030001"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF29">
        <property type="entry name" value="PUMMELIG, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="149"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="47432" checksum="BFD4408CD9C830A6" modified="2016-10-05" version="1">MSSAVQVRFFSGWPRPSTNFLRLASSGQAGFRIENHGTARQPLSRHKYKRPGHPTAVPLHKLFSSQFPLSLRQLVEDYAMRDNPLRLQHDLLLALPFFPGPDGRGRRGEVVQTRLLSGERINEFVIYPASRAGQHVAPGSRACRSVKHLIMVHGYGAGLGFYLKNFEALLALDDWVVHAVDLLGYGCSSRPAFTAQSLAAVERFFHDSFGEWLRARGLAERPQQNMVVAHSMGAYLMATYGIQKQRDFCAKLVMVSPGAVIKHRRAVVVPPYFARLWERNISPFSLVRNAGPLGSKLVSMWSSRRFARLPPAEARLLHRYAYGIFQSPGSGEYMLNFLLAPGADARHPLVSRGIEKLACDVLWCYGKDDWMDKAGGELCSALINRRAGSAVKSTTCEIDNAGHHVYLDNPAEFNELVLRQMRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIB6</accession>
    <name>A0A1A0HIB6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_30063</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714108.1">
        <property type="nucleotide sequence ID" value="XM_018855545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIB6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIB6"/>
    <dbReference type="GeneID" id="30028521"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
        <property type="entry name" value="RNA_pol_Rpa2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="18">
        <location>
            <begin position="30"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="189"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="461"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="20">
        <location>
            <begin position="567"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="15">
        <location>
            <begin position="674"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="1039"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA23627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1168" mass="130311" checksum="6E4BD67AA4476F26" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIF3</accession>
    <name>A0A1A0HIF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_37206</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714099.1">
        <property type="nucleotide sequence ID" value="XM_018856840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIF3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIF3"/>
    <dbReference type="GeneID" id="30029816"/>
    <dbReference type="OrthoDB" id="6703404at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051508">
        <property type="entry name" value="Mito_Carrier_Antiporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45928:SF1">
        <property type="entry name" value="RE38146P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="15"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="127"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="232"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="326" mass="36027" checksum="FC281D30B135B6DC" modified="2016-10-05" version="1">MVEKTEQKKNLAQEVSLLGGFIAGSIAACGAVTFTNPIELIKTRMQLQGELSLKSDAPRLYKNPLQASLVIYKNEGLRGLQQGLICGYVYQIALNGCRLGLYEPTRYYMTKVLLPDQFSEDASRIPQNLFVNVMSGIVSGWAGAVISNPFFLIKTRMQSFNKAGASGSVGQQTYYRGLFDGLKTIYANEGIKGWFRGTDAALLRTGAGSAAQLPVYNFTKNYILKHDLMTDGSVGLHLVSSSMTGLGVAIVMNPWDVVLTRVYNQKGNLYTGPIDCFIKTIKTEGPTALYKGFWAQLFRIGPHSILMLLFMEQSMKLLHTVEKRFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH8</accession>
    <name>A0A1A0HIH8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Septin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34056</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714296.1">
        <property type="nucleotide sequence ID" value="XM_018856435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH8"/>
    <dbReference type="GeneID" id="30029411"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990317">
        <property type="term" value="C:Gin4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001400">
        <property type="term" value="C:mating projection base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072687">
        <property type="term" value="C:meiotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031105">
        <property type="term" value="C:septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032160">
        <property type="term" value="C:septin filament array"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000281">
        <property type="term" value="P:mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="6">
        <location>
            <begin position="19"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="328"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="392" mass="45043" checksum="C0975F21714CAFB0" modified="2016-10-05" version="1">MEDYNTSSSALRKRKTIKKTINFSFMVIGESGLGRSTLINSLCGGNQIVPPLLTATQDAFHKKLTLRHENVELEDNDGHKISLNIIDTPNFANGLDCGDDFKVVVDFIRHQYDEVLLEESRVKRNPRFKDGRIHVLLYMVRATGHGLSPIDVQFLQHVSGLVNVIPVICKADALTPAELRLNKRLVLEDLARHNIHYYKFNEYEYEQDYMDDEIVEYNKYLNLLVPFAVIGANSLEALADDDDPVKLRVLNPAYAVPINVELPEFNDFTILKNVLLITHLNEFKEITHDVIYENYRTEALSGKQFHYSAGDARLGEDLPEPEPGASDYLAKEEQIKLEEERLRKFEERVHQDLINKRKELAERENELREIEKRLLAEGLKLNDDGDVVKLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIM1</accession>
    <name>A0A1A0HIM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Methylenetetrahydrofolate reduct</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_9932</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714169.1">
        <property type="nucleotide sequence ID" value="XM_018859492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIM1"/>
    <dbReference type="STRING" id="869754.A0A1A0HIM1"/>
    <dbReference type="GeneID" id="30032467"/>
    <dbReference type="OrthoDB" id="16284at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004489">
        <property type="term" value="F:methylenetetrahydrofolate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000002">
        <property type="entry name" value="Methylenetetrahydrofolate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004621">
        <property type="entry name" value="Fadh2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053806">
        <property type="entry name" value="MTHFR_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00677">
        <property type="entry name" value="fadh2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45754:SF1">
        <property type="entry name" value="METHYLENETETRAHYDROFOLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21895">
        <property type="entry name" value="MTHFR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MTHFR SAM-binding regulatory" evidence="9">
        <location>
            <begin position="363"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="631" mass="71245" checksum="A6D2F20469FF4810" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIM2</accession>
    <name>A0A1A0HIM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">ATP-dependent metallopeptidase Hfl</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_76910</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714485.1">
        <property type="nucleotide sequence ID" value="XM_018858606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIM2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIM2"/>
    <dbReference type="GeneID" id="30031582"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000002">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000175">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048438">
        <property type="entry name" value="Yme1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21232">
        <property type="entry name" value="Yme1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="198"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA24004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="629" mass="68337" checksum="9C7C73128D74A694" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIN7</accession>
    <name>A0A1A0HIN7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit I</fullName>
            <shortName evidence="7">eIF3i</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 39 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF-3 39 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF34</name>
        <name evidence="9" type="ORF">METBIDRAFT_37343</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714184.1">
        <property type="nucleotide sequence ID" value="XM_018856861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIN7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIN7"/>
    <dbReference type="GeneID" id="30029837"/>
    <dbReference type="OrthoDB" id="24966at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000127">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03008">
        <property type="entry name" value="eIF3i"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="6"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="48"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="292"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="356" mass="39417" checksum="E2D74B5569DC134B" modified="2016-10-05" version="1">MRPIKLMGHQRSLTQVKFNREGDIIFSVAKDSLALIWYSSNGERLGTLEGHQGTIWSIDVDLKTELCATGSADLSIRLWNVQTGENVFTWDAASPVRRVAFSPDNLQLLAVNDKVMGSKGAISVYDINYGNVHDQQVEPRLVIETRPDATKVTVAGWAAEGKYIIAGHEDGFVSKYDAATGEFLQTVQAHGIHTEEKVVSVTDIQFAQDDNLYFITSSKDKTAVILDVDTLEILKVYKADAPMNTAAITPRKNFVILGGGQEARNVTTTLESQGKFEARFYHKIFTDEIGRVKGHFGPLNTVAVHPDDMGYVSGGEDGFIRVHYYDKSYFDFEFDAERTERVLAQAQANATPQAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIR5</accession>
    <name>A0A1A0HIR5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_116843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714530.1">
        <property type="nucleotide sequence ID" value="XM_018854060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIR5"/>
    <dbReference type="GeneID" id="30027036"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="83" mass="9626" checksum="68E8201C4C985547" modified="2016-10-05" version="1">MLVIDQGSRSSSLAALLLAMALYEDQKELQPFPLAKKQRQIYVHVYLLSQPVTYVNVYILDTRPVQQRHTRPCFWQEKTCCVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ17</accession>
    <name>A0A1A0HJ17_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_30222</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714314.1">
        <property type="nucleotide sequence ID" value="XM_018855577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ17"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ17"/>
    <dbReference type="GeneID" id="30028553"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000013">
        <property type="entry name" value="Proteasome subunit beta type-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="283" mass="30933" checksum="9573129D03B315E9" modified="2016-10-05" version="1">MNTIASQYTHAAQSQLHNEYSTGTANLAASTPQLLLAPSIEVPQILKPAEFLRAHTDHAANPDCKIKIAHGTTTLAFRFQGGIVVAVDSRATAGNWVASQTVNKVIRINPFLLGTMAGGAADCQYWETWLGTQCRLHELREKERISVAAALKILGNLVYQYKGMGLSMGTMVCGYTKKEGPAIYYVDSDGTRMKGDMFCVGSGQTFAYGVLDSEYNWDMSVEDALYLGKRSILAAAHRDAFSGGSINLYHVTESGWVYHGNHNVGDIFYDIKETEKSFNNVDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ33</accession>
    <name>A0A1A0HJ33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714492.1">
        <property type="nucleotide sequence ID" value="XM_018853708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ33"/>
    <dbReference type="GeneID" id="30026684"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013243987" evidence="1">
        <location>
            <begin position="16"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="147" mass="16182" checksum="0F44C3DD1BB19246" modified="2016-10-05" version="1">MKLLATLPLLSLVLALPGPSLPLSGFLSIKKSGDPAQVGTGYISINDDKVVKSDTSYKFDSEKGLVFLYRLGRGPNKLFMGLDEQKKLALTEQEPVVHIITKDAHEHVLSVGGNFKFELCDDGLIGQNSGCTFREDIEIKWTPYLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJB8</accession>
    <name>A0A1A0HJB8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADP(+)-dependent dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_117972</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714591.1">
        <property type="nucleotide sequence ID" value="XM_018854092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJB8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJB8"/>
    <dbReference type="GeneID" id="30027068"/>
    <dbReference type="OrthoDB" id="6251714at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000047">
        <property type="entry name" value="NADP-dependent L-serine/L-allo-threonine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42901:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="268" mass="28522" checksum="4B8DE3FB74EB01E0" modified="2016-10-05" version="1">MSFGEKAAVRLANKIVLITGASAGIGAATAHEFASAANGHIKLILTARRESRLAELADELTKAYAPIKVYTAKLDVSDTASIKPFVASLPAEFADIDILINNAGKALGRETVGDIDTDDVAGMMQTNVVGLINMTQAVLPIFKRKNAGDIVNIGSIAGREPYPGGSVYCATKAAVNFFTHSLRKELINTKIRVLEVDPGAVETEFSVVRFRGDKAAADKVYEGTEPLIAQDIAEVIVFGVSRRQNTVIAETLVFPSNQAGSAHIHRNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD9</accession>
    <name>A0A1A0HJD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL46</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL46 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714483.1">
        <property type="nucleotide sequence ID" value="XM_018855624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJD9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJD9"/>
    <dbReference type="GeneID" id="30028600"/>
    <dbReference type="OrthoDB" id="414075at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04661">
        <property type="entry name" value="NUDIX_MRP_L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040008">
        <property type="entry name" value="Ribosomal_mL46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021757">
        <property type="entry name" value="Ribosomal_mL46_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033650">
        <property type="entry name" value="Ribosomal_mL46_NUDIX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L46, MITOCHONDRIAL PRECURSOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13124:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11788">
        <property type="entry name" value="MRP-L46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291827" description="Large ribosomal subunit protein mL46" evidence="8">
        <location>
            <begin position="36"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein mL46 N-terminal" evidence="9">
        <location>
            <begin position="20"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="265" mass="30648" checksum="CBD36125F9D99D4C" modified="2016-10-05" version="1">MWKQAFIRWNSTAAAVAPVQIRLTLLLLRIPVVSADLPVFQQQYYKYQNELWKRLMWTFPKWFYFREGTLAEQKYRELNKDPVFNNPNLEFTGGRPEIRHQRDRRFKQDVLLPKTYKEGEETTQADAMSRKIVPNLRTTDADRHNDESSLERRLARTLYLVVSADKGQTWGFPTFASDGQALHTTAEAGLYALGGTQINYFNVSPKPCHVLSAGDSRAFFIKLHILSGVFQPAPNTVHKWLAKDELAAHLDELYYNDVCHLLSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF9</accession>
    <name>A0A1A0HJF9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_116242</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714503.1">
        <property type="nucleotide sequence ID" value="XM_018854039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF9"/>
    <dbReference type="GeneID" id="30027015"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="138" mass="15470" checksum="8D8FA2128AAC22C8" modified="2016-10-05" version="1">MPVPISPCCPFPALASRSPLPAWSSTRPSARRRRICLGARRPGHPQKQATRRVSANPLTARHTTNEQNPAHKIQPTKSSPQNPAHKIQTNPQKQPSKSNPQNPTHTLTRNKNQKPNPKNHTRFKRRLEYPRPPGPGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJI0</accession>
    <name>A0A1A0HJI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_77011</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714637.1">
        <property type="nucleotide sequence ID" value="XM_018858621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJI0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJI0"/>
    <dbReference type="GeneID" id="30031597"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033255">
        <property type="term" value="C:SAS acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046972">
        <property type="term" value="F:histone H4K16 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF219">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="14">
        <location>
            <begin position="26"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="13">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA24156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="347" mass="39025" checksum="FC857A31D5A22F86" modified="2016-10-05" version="1">MQKNPPLHVGDSQHASRHDVAEYGRLESRNIGTVTFGDYVIDTWYGNSAYFQNFGDSKLGVDPEVVHSRRRGFLPVKPGFWVDNLYVCDSCFKYTLNAEHMAAHAPACPLQKRFPPLGKVVYVDKRQPYVIKKVRGYRHPLFCQNLALFGKLFLDDKLVFYNVEAFDFHVLYGHVPGARARPAGHLSPLKPMGFFSAEVNSYELDTNLACICIFPPFQRLGLGSLLIEFLYAMARVTPGQQFLGPEYPLLPFGRRLYLRFWARRLAFAVFAELGLQDAVTLLDLARLTGFRKEDVLVALEYMGVLQRGGPGDAEATLLVSSTRAWCDANGVDPKTLHCMLDPDGVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJK5</accession>
    <name>A0A1A0HJK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_38487</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714499.1">
        <property type="nucleotide sequence ID" value="XM_018857010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJK5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJK5"/>
    <dbReference type="GeneID" id="30029986"/>
    <dbReference type="OrthoDB" id="10256606at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04330">
        <property type="entry name" value="RNAP_III_Rpc25_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000221">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="7">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="8">
        <location>
            <begin position="83"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="196" mass="22513" checksum="DC5EC939651879EE" modified="2016-10-05" version="1">MFILSQMSDLVRVPPQSFNMPIHHVLKNELHQKYSNKVITNVGLAVAVWDLLEIKDGLLRPGDGASYVEVKFRMVVWKPFIGEILSGWVADCSPEGIKVRMEFFEEIFIPKDYLFESCIFKPVEKAWVWKPDDESELFIDINEKIRFRIEEEIFVNIKPQSSSEALGLDEAPNKAPPYALIASCQTEGMGCVSWWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJL2</accession>
    <name>A0A1A0HJL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_36923</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714835.1">
        <property type="nucleotide sequence ID" value="XM_018856800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJL2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJL2"/>
    <dbReference type="GeneID" id="30029776"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044773">
        <property type="term" value="P:mitotic DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001100">
        <property type="term" value="P:negative regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060903">
        <property type="term" value="P:positive regulation of meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905263">
        <property type="term" value="P:positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905342">
        <property type="term" value="P:positive regulation of protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904968">
        <property type="term" value="P:positive regulation of spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF23">
        <property type="entry name" value="CDC7 KINASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="168"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="91"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="585" mass="64756" checksum="9C27CE5868DDD42D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJU3</accession>
    <name>A0A1A0HJU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA N-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_34100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714925.1">
        <property type="nucleotide sequence ID" value="XM_018856442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJU3"/>
    <dbReference type="GeneID" id="30029418"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051556">
        <property type="entry name" value="N-term/lysine_N-AcTrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42919:SF8">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="19867" checksum="0F59C123E3F1EBB7" modified="2016-10-05" version="1">MGRSVITLDDLTPNNLGTFKKINEVSLPTSYPESWYKESLDSAQIVKLAFYNELPVGAIKGKLFHTSHKLPNFELSTKSNMNTKIVMNALYLESLAVLKAYRNQGVASELLQWIISEAKSRFVHEIFLHVHVENDEAIQWYKKKGFVQKDEVVKDYYKEQGLPQPDAAILSISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJV5</accession>
    <name>A0A1A0HJV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_34791</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Exact function not known, phosphatase 2A performs an essential cellular function.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714762.1">
        <property type="nucleotide sequence ID" value="XM_018856527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJV5"/>
    <dbReference type="GeneID" id="30029503"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990813">
        <property type="term" value="P:meiotic centromeric cohesion protection in anaphase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903432">
        <property type="term" value="P:regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000003">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="10">
        <location>
            <begin position="142"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="38464" checksum="36B19A70D54BD86C" modified="2016-10-05" version="1">MDLDTDVPMEDAVEDVQLTKDAALSKKPDVSISSINQLDQWIEKLTQCEPLSEADVKRLCEMAIDVLQFEENVQPVQVPVTICGDVHGQFHDLMELFKIGGPCPDTNYLFMGDYVDRGYYSVETVSYLVCMKVRYPNRITILRGNHESRQITQVYGFYDECLRKYGSAAVWKLFTDLFDYFPITALVDNKVFCLHGGLSPMIETIDQVRELNRIQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGFTFGQDISEQFNHTNDLSLIARAHQLVMEGFSWSHQESVVTIFSAPNYCYRCGNQAAIMEVDENHQRQFLQYDPSVRPGEPTVTRKTPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX3</accession>
    <name>A0A1A0HJX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear protein localization protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77125</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the import of nuclear-targeted proteins into the nucleus and the export of poly(A) RNA out of the nucleus. Has a role in the endoplasmic reticulum-associated degradation (ERAD) pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NPL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714782.1">
        <property type="nucleotide sequence ID" value="XM_018858644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX3"/>
    <dbReference type="GeneID" id="30031620"/>
    <dbReference type="OrthoDB" id="10251089at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036266">
        <property type="term" value="C:Cdc48p-Npl4p-Vms1p AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030894">
        <property type="term" value="C:replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900182">
        <property type="term" value="P:positive regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024682">
        <property type="entry name" value="Npl4_Ub-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11543">
        <property type="entry name" value="UN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="245"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="573" mass="63214" checksum="3242EF3DD805F1EB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKC1</accession>
    <name>A0A1A0HKC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">26S proteasome subunit P45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_38379</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714932.1">
        <property type="nucleotide sequence ID" value="XM_018856995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKC1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKC1"/>
    <dbReference type="GeneID" id="30029971"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000008">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000034">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="217"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="440" mass="48980" checksum="2DF6CFFD2F5589A2" modified="2016-10-05" version="1">MSQDSDPLLAALHNDGAEQTPEQAAQTAAQTAAQTEQPAQPEQPEQPEQAAEHQERVRALAKFKDKLVEHRKWDARLKDMRLSMRDLLASYDKTEDDIKALQSVGQIVGEVLKRLSDDTYIVKASSGPRYIVGCRNTIPRHGLQNGVRVSLDMTTLTIMRLLPREVDPLVYNMTTFEPGEISFNGIGGLAEQIRELREVIELPLKNPELFTRVGIKPPKGVLLYGPPGTGKTLLAKAVAATIGANFIFSPASAIVDKYIGESARLIREMFAYAKEHEPCIIFMDEIDAIGGRRFSEGTSADREIQRTLMELLNQMDGFDTLGQTKVIMATNRPDTLDPALLRAGRLDRKIEISLPNEAGRLDIFKIHTAKVSKQGDFDFEAAVKMSDGFNGADIRNVITEAGFFAIRDERDYIVQNDLMKAVRKVADVKKLEGKLDYEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKH9</accession>
    <name>A0A1A0HKH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_35348</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714794.1">
        <property type="nucleotide sequence ID" value="XM_018856600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKH9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKH9"/>
    <dbReference type="GeneID" id="30029576"/>
    <dbReference type="OrthoDB" id="431497at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051177">
        <property type="term" value="P:meiotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03272">
        <property type="entry name" value="ABC_SMC3_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000370">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000424">
        <property type="entry name" value="Structural maintenance of chromosomes 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041741">
        <property type="entry name" value="SMC3_ABC_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="10">
        <location>
            <begin position="525"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="178"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="679"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="879"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="994"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1193" mass="136314" checksum="1A86CCD60AECABA0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKU5</accession>
    <name>A0A1A0HKU5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018853803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKU5"/>
    <dbReference type="STRING" id="869754.A0A1A0HKU5"/>
    <dbReference type="GeneID" id="30026779"/>
    <dbReference type="OrthoDB" id="3260408at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="650"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="696"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="720"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="767"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="783" mass="89734" checksum="F997B393AB9E89DE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ67</accession>
    <name>A0A1A0GZ67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA16985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709282.1">
        <property type="nucleotide sequence ID" value="XM_018856402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ67"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ67"/>
    <dbReference type="GeneID" id="30029378"/>
    <dbReference type="OrthoDB" id="28413at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16457">
        <property type="entry name" value="PH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="1">
        <location>
            <begin position="452"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA16985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="664" mass="76340" checksum="A2048B64706A1E08" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZA5</accession>
    <name>A0A1A0GZA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">SNF2-family ATP dependent chromatin remodeling factor snf21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_48003</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709322.1">
        <property type="nucleotide sequence ID" value="XM_018858249.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0GZA5"/>
    <dbReference type="GeneID" id="30031225"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014978">
        <property type="entry name" value="Gln-Leu-Gln_QLQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51666">
        <property type="entry name" value="QLQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="QLQ" evidence="17">
        <location>
            <begin position="185"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="HSA" evidence="16">
        <location>
            <begin position="495"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="673"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="986"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="1362"/>
            <end position="1432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="236"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="606"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1298"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="1138"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="236"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1312"/>
            <end position="1323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA17025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1497" mass="169895" checksum="D98B9157C78DF30B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Q2</accession>
    <name>A0A1A0H1Q2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">low-specificity L-threonine aldolase</fullName>
            <ecNumber evidence="8">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_47912</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709354.1">
        <property type="nucleotide sequence ID" value="XM_018858237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Q2"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Q2"/>
    <dbReference type="GeneID" id="30031213"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06502">
        <property type="entry name" value="TA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA17959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="372" mass="40538" checksum="A6C7AF69204F9367" modified="2016-10-05" version="1">MSLDLSVFTESPAANEFRSDTFTTPTASMVSALATTTLGDSVYNEDSTTRELEMKVAELAGKEVGLFCVSGTLLNQIGLRTHLLQPPHAVLCDHRAHVYVHEAGGLPTLSQAMVHSIVPKNGVYLTLEDDILPNYVPDDGEIHGCPTKVISLENTLHGMLFPLDEIKKISAFCKDNNVRLHLDGARLWNASAATGISIAEYCSYFDSVSLCLSKTLGAPVGSVLVGTKKFVSKANHFRKQNGGGIRQAGLLALMAIVAIDENFPKLKKTHDVAKELGEFCESLGAVLEHPVHTNFVFIDIAASKINNDFFTQACAKYNVKAYGSRLTIHHQVSEEGLANLKRALTDTLEEARKNPYDAKASLKLYNVYGKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Y5</accession>
    <name>A0A1A0H1Y5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">40S ribosomal protein S3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_226261</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709360.1">
        <property type="nucleotide sequence ID" value="XM_018855177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Y5"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Y5"/>
    <dbReference type="GeneID" id="30028153"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1140.32:FF:000013">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000006">
        <property type="entry name" value="40S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="8">
        <location>
            <begin position="23"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA17965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="239" mass="26266" checksum="5410FAC533AD54E4" modified="2016-10-05" version="1">MSAQQILSKKRKLVADGVFFAELNEFFTRELAEQGYAGVEVRKTPTKLEVIVKASNTQGVLGEQGRRIHELTSLIVKRFRLSPEGIVIYAERVEERALSAAVQAEALKAKLLSGLPIRRAAYGVLRFTMAAGAKGVEVVISGKLRAARAKSQKYADGFMIHSGQPTKDFIDSAIRHVLMRQGVLGIRVKIMRDPSKNKFGPTSLPDAVKISEYKEEDEVVPAPTVNVYRQKEAAAEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H209</accession>
    <name>A0A1A0H209_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_47738</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in the mitochondrial lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709388.1">
        <property type="nucleotide sequence ID" value="XM_018858212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H209"/>
    <dbReference type="STRING" id="869754.A0A1A0H209"/>
    <dbReference type="GeneID" id="30031188"/>
    <dbReference type="OrthoDB" id="61815at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001151">
        <property type="entry name" value="Large subunit GTPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="8">
        <location>
            <begin position="190"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="626"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="636"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA17993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="655" mass="74245" checksum="7E5A2DF9A2C68A9C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4U6</accession>
    <name>A0A1A0H4U6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33587</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709483.1">
        <property type="nucleotide sequence ID" value="XM_018856340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4U6"/>
    <dbReference type="STRING" id="869754.A0A1A0H4U6"/>
    <dbReference type="GeneID" id="30029316"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="73"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="282"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="327"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="376"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="474" mass="52764" checksum="2A3CEA46929928A4" modified="2016-10-05" version="1">MGEFEDPYGDDFESDEEIIELDSAGEEEEDQGMEEDAALGKIEEVDIEKFSQQESAPESTLYLPHRSKPLGPDEVLEADPTVYEMLHNVNMPWPCLTLDIMPDNLGSERRSYPASVYVATATQASKAKDNELIAMKLSSLSKTLVKEDEEDEEDEDLDDELHVDPVMMSETVPLKSTTNRVRVSPHARETGEYLTALLQENGEVQIFDLGSQFKAFDSPGYMIPKTAKRPLHTVRVHGNVEGYGLDWSPLINTGSLLTGDCLGRIHLTSRTTSSWVTDKTPFTASDHSIEDIQWSTGENTVFASAGCDGYVRIWDTRSKKHKPVISVAASTTDVNVISWSDKINHLLASGHDDGSWGVWDLRNFGGKETPAPVAHYDFHKSAITSISFNPLDESIIAVSSEDNTVTLWDLAVEADDEEIAQQRKEAQELSDIPPQLLFVHWQRDVKDVRWHKQIPGCLVSTGGDGLNIWKTISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H536</accession>
    <name>A0A1A0H536_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_17053</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709600.1">
        <property type="nucleotide sequence ID" value="XM_018854688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H536"/>
    <dbReference type="STRING" id="869754.A0A1A0H536"/>
    <dbReference type="GeneID" id="30027664"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051643">
        <property type="entry name" value="Transcr_Reg_ZincFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396:SF19">
        <property type="entry name" value="FI01119P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24396">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="52"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="367" mass="41692" checksum="57E786A8E80098ED" modified="2016-10-05" version="1" fragment="single">PAISRELPEKPIVEAFPQPCSTGLTTRMLPFKNNKGEIEWVLTDDVQPGRELDEFKANPDTFKTAPPLSKTVKNSKTSAILSPVTSNSSNNESIPEKKNDSSVSPQINTPSSSSSEDHKEDMKEGEVDSESGPLEHECPHCDAKFRMRGYLTRHLKKHMPQKAYQCPFREKSLYKDENDNMHQCHPSGGFSRRDTYKTHLKTRHFKFPSGTPIKGRSQSSGNCSMCGEWFENAEIWSEIHVEGAECKYLPEGFQGKSRIKNKIKKQMARLMREEKKSKLRSKKSSTFDYVNPSQYEYEDSPTQSSCSSMGPQRSAHPVFHDPFPRDTSQRSFYANSPLSDQNLVVPPLTQADDFDDEFCLDTEQMSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H562</accession>
    <name>A0A1A0H562_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Alpha-1,3-glucosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33666</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ALG6/ALG8 glucosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709582.1">
        <property type="nucleotide sequence ID" value="XM_018856371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H562"/>
    <dbReference type="STRING" id="869754.A0A1A0H562"/>
    <dbReference type="GeneID" id="30029347"/>
    <dbReference type="OrthoDB" id="5589195at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042281">
        <property type="term" value="F:dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004856">
        <property type="entry name" value="Glyco_trans_ALG6/ALG8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413">
        <property type="entry name" value="DOLICHYL GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12413:SF1">
        <property type="entry name" value="DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03155">
        <property type="entry name" value="Alg6_Alg8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="368"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="504"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="558" mass="63838" checksum="DC2BB922A56E8D46" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5T8</accession>
    <name>A0A1A0H5T8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33504</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709852.1">
        <property type="nucleotide sequence ID" value="XM_018856313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5T8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5T8"/>
    <dbReference type="GeneID" id="30029289"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03337">
        <property type="entry name" value="TCP1_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000073">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000085">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="526" mass="58032" checksum="93C6F70ED69A8700" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5U6</accession>
    <name>A0A1A0H5U6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Photolyase/cryptochrome alpha/beta domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_46460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="6">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709938.1">
        <property type="nucleotide sequence ID" value="XM_018858043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5U6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5U6"/>
    <dbReference type="GeneID" id="30031019"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043153">
        <property type="term" value="P:entrainment of circadian clock by photoperiod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.579.10:FF:000003">
        <property type="entry name" value="Deoxyribodipyrimidine photo-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018394">
        <property type="entry name" value="DNA_photolyase_1_CS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF18">
        <property type="entry name" value="SI:CH1073-390K14.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00394">
        <property type="entry name" value="DNA_PHOTOLYASES_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00691">
        <property type="entry name" value="DNA_PHOTOLYASES_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4 6" id="KW-0274">FAD</keyword>
    <keyword evidence="3 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="8">
        <location>
            <begin position="61"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="352"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="450"/>
            <end position="452"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="437"/>
        </location>
    </feature>
    <feature type="site" description="Electron transfer via tryptophanyl radical" evidence="7">
        <location>
            <position position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="556" mass="64843" checksum="A8AA5FA046111D7A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H668</accession>
    <name>A0A1A0H668_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1765-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709934.1">
        <property type="nucleotide sequence ID" value="XM_018859148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H668"/>
    <dbReference type="STRING" id="869754.A0A1A0H668"/>
    <dbReference type="GeneID" id="30032124"/>
    <dbReference type="OrthoDB" id="296767at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013887">
        <property type="entry name" value="UPF0592"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37988:SF1">
        <property type="entry name" value="UPF0592 MEMBRANE PROTEIN C7D4.03C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08578">
        <property type="entry name" value="DUF1765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="734"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="977" mass="108722" checksum="1D668C1244B5D069" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6A0</accession>
    <name>A0A1A0H6A0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cu,Zn superoxide dismutase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_28208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710142.1">
        <property type="nucleotide sequence ID" value="XM_018855349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6A0"/>
    <dbReference type="STRING" id="869754.A0A1A0H6A0"/>
    <dbReference type="GeneID" id="30028325"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="1">
        <location>
            <begin position="27"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="156" mass="17178" checksum="A2FF168D53736332" modified="2016-10-05" version="1" fragment="single">APLSKDNPKNVVAVADFPESGTNKFTKGYVYFTSPKGSSVKVHVDMTGLPPVDGPFVYHIHEGQIFDKVSCDEAGKHFDPFRGLEKCTENSDDALCMVGDLSGKHGWINATCFQTEYHDKYLSLNPASESYIVGRSIVFHDQKLTRFACANINLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6D6</accession>
    <name>A0A1A0H6D6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inactive metallocarboxypeptidase ECM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Inactive metallocarboxypeptidase ecm14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_79600</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Inactive carboxypeptidase that may play a role in cell wall organization and biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the peptidase M14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710119.1">
        <property type="nucleotide sequence ID" value="XM_018859096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6D6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6D6"/>
    <dbReference type="GeneID" id="30032072"/>
    <dbReference type="OrthoDB" id="3626597at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03860">
        <property type="entry name" value="M14_CP_A-B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000060">
        <property type="entry name" value="Putative metallocarboxypeptidase ecm14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF147">
        <property type="entry name" value="INACTIVE METALLOCARBOXYPEPTIDASE ECM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011955427" description="Inactive metallocarboxypeptidase ECM14" evidence="13">
        <location>
            <begin position="16"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M14" evidence="14">
        <location>
            <begin position="157"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="470" mass="53354" checksum="565B4781D3113551" modified="2016-10-05" version="1">MKVALLAYVFAVVASQQIPFFLDTSNELPLRPHYSSLDSSELPIDLSQYMDNVVVRVNQSANILHHIKSRGHKIWAQNSKAGTVDVQLHSNDAAAFFAEISVASPQVLIRDLPQAIFETFPDEMTVGNTEPIFAAQLTPGMLALEKLHVLSEVFFKQFRPLSSINAWLALLQQTYPDIITLETIGQTYEGRPLQVVHLLIPSDNTSHDEKRTIVVTGGVHAREWISVSSVLYGIYEILNHFESNPDNWRELAKLDFLFIPVLNPDGYEYSWSTDRLWRKNRQPAAGSEDSQCRGIDIDHSYDFHWTPSSDSICGEDYAGSHPFEAYELKVWDTYLNRTNEGHKIWGYIDLHSYSQEILIPYAYSCEHRPRDEENLIELAYGISKAIRLTSGKYYSVLPACIDRDSDLIPDMGAGTALDYMYHQKSYWAYQIKLRDNGSQGFLLPAKYIVPVGKEISAGLKVFCKFILSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L3</accession>
    <name>A0A1A0H6L3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_45972</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710128.1">
        <property type="nucleotide sequence ID" value="XM_018857977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6L3"/>
    <dbReference type="GeneID" id="30030953"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847:SF150">
        <property type="entry name" value="OXIDOREDUCTASE TDA3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13847">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="3">
        <location>
            <begin position="21"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="468" mass="51115" checksum="C0D3D7EFC3223425" modified="2016-10-05" version="1">MSDYLQFRTTALGRHEGKTHIVIVGAGIIGVCTAYFLVKHPKFDPSKHHISLVESKRVAGGASGKAGGLLALWAFPLQLVPLSFELHQKLSDLYDGEKEWDYRRLTTLSIEGDISGVPDEVAEDEGFEKPEHSKSIKNGDKPPKKTLRATSVEDKKILPPDLTWMSEELVTGCHSLGGKDTTAQVHPYKFTNFLLRKAVEDSDGALELILGKVNKVLYLMETGGVTGIQYEPTSVKAAQSEQALGPIRLNGDQVVLSVGPWTSKILPDCPISGLRAHSITVEPFKSESVSPYAIFTEYKAGKDSYVSPEIYARKDEVYVCGEGDSAVDVPETTDDVEVVKSKCDDIFRQAGKMSPMLRNGRILKRQACYLPVLDVPSSSGPLIGETNVENLYLCSGHSCWGINNAPGSGKIMSELLLDGRASSANISSLDPSLYFDASVLVSDDEDDDEYDEDDDEYDEDEEAVVDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6M4</accession>
    <name>A0A1A0H6M4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710204.1">
        <property type="nucleotide sequence ID" value="XM_018854357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6M4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6M4"/>
    <dbReference type="GeneID" id="30027333"/>
    <dbReference type="OrthoDB" id="5581181at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044554">
        <property type="entry name" value="ANAPC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057975">
        <property type="entry name" value="TPR_ANAPC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45957:SF1">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25773">
        <property type="entry name" value="TPR_ANAPC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="2">
        <location>
            <begin position="379"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="623" mass="69836" checksum="153571067DE8D31A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6M6</accession>
    <name>A0A1A0H6M6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inorganic pyrophosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_45862</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710090.1">
        <property type="nucleotide sequence ID" value="XM_018857967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6M6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6M6"/>
    <dbReference type="GeneID" id="30030943"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000004">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="284" mass="31679" checksum="47918C45A4774E3E" modified="2016-10-05" version="1">MSYTTRQIGAANTLDYKVYIEKDGKVVSPFHDIPLYANKEKTILNMIVEVPRWTNAKLEISKEQKLNPIIQDTKKGKLRFVRNCFPHHGYIHNYGAFPQTWEDPNQVHPETKAKGDDDPLDVCEIGEQVAEVGQVKQVKVLGVMALLDEGETDWKVIVIDVNDPLAPKLNDIEDVETHLPGLLRATNEWFRIYKIPDGKPENQFAFSGECKNKKYADEVVAECAEAWDKLIKGSTAPGEIDLTNSTLSSTASFSEAAEVPAASPLPAAPIDKSVDKWFFISGAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6N7</accession>
    <name>A0A1A0H6N7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zf-UBP-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_32825</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710279.1">
        <property type="nucleotide sequence ID" value="XM_018856136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6N7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6N7"/>
    <dbReference type="GeneID" id="30029112"/>
    <dbReference type="OrthoDB" id="273556at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045471">
        <property type="term" value="P:response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16457">
        <property type="entry name" value="RING-H2_BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12717">
        <property type="entry name" value="RRM_ETP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011422">
        <property type="entry name" value="BRAP2/ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034931">
        <property type="entry name" value="ETP1_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047243">
        <property type="entry name" value="RING-H2_BRAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24007:SF7">
        <property type="entry name" value="BRCA1-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07576">
        <property type="entry name" value="BRAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="233"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="UBP-type" evidence="8">
        <location>
            <begin position="270"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="552"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="460"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="566"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="576" mass="65345" checksum="9DD955D9AD8230EF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6W7</accession>
    <name>A0A1A0H6W7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromatin target of PRMT1 protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710364.1">
        <property type="nucleotide sequence ID" value="XM_018857803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6W7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6W7"/>
    <dbReference type="GeneID" id="30030779"/>
    <dbReference type="OrthoDB" id="5382468at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="177" mass="19384" checksum="CEDEAAFC8388F30B" modified="2016-10-05" version="1" fragment="single">RPYNGTGVPAQILNSAEGRAMLRLKNIHPDLNGMDLSDLFVNIAPVDFVKFDPRDETIAYVCFQLNFAENNARAIQKFDGKKAMGNTLIVENATSLADRIAPLPAGRMPRGSRDSVRTGDDAKVKQQTEKRARPVPKTAEDLDKELIAYMNGASENNVKLESTGAFAAQDNGEAMME</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Z9</accession>
    <name>A0A1A0H6Z9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710210.1">
        <property type="nucleotide sequence ID" value="XM_018858000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Z9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Z9"/>
    <dbReference type="GeneID" id="30030976"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="230"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="39836" checksum="83BF6E9316307038" modified="2016-10-05" version="1" fragment="single">MSAGKPKTANYRGPLGVPYRPDLGLEKSSLSMRLMHRPSRAQKELEKASDPTRKNSLLNSLCAPDRDSDSEQVSPQEVRSHLSVSEDALSLDSTPLCLPRHNLVRKKSGEIVKPLLKDKTLPVNMRSRSLPSTPTYKQVHFGGDTDVRYFKKKDKPNAILAQNSPKLYDEDDDDDDENHEIPSLEFEGDLRMQSLFNPNRKSYFEQPQLPSPKDWLLDPLNFSPRENALTYIRKGKQVFLENIFLSADKRYLLGQVAVQNIDFEKSVTIRYSVDKWCTIVEIQAIYIPDIPHILKAHNYDRFMFKISLDLFVNGFHTSVDPLSTAPPDFLAELCVKYRVNGQELWDNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H720</accession>
    <name>A0A1A0H720_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710356.1">
        <property type="nucleotide sequence ID" value="XM_018857821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H720"/>
    <dbReference type="GeneID" id="30030797"/>
    <dbReference type="OrthoDB" id="4078061at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="255" mass="28195" checksum="0F01D0B1D3C4D497" modified="2016-10-05" version="1">MGNKASKPARKLTGSLSNAGKINNPSSLRIQLPSQELKDRFENSGAELAQANEKMAKSNHHTSNDPDSNVERGTQGSATVANGPEGKDGMDPQADQNYINFINNLGRQIHSHSEGNKGEKVNVRALKQLLNRKNLHKKGQDEVQAQLESTSTTRSMVHPRTLTAIIYALHDPRVSHDNVMKDYQLSPEFLNNMDRFKVAENMVVIEDDIKEDEIGPKFGQPVSRAASDPSLMNENGAVGESVNSDRMKKLRSRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H787</accession>
    <name>A0A1A0H787_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_72613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710416.1">
        <property type="nucleotide sequence ID" value="XM_018858405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H787"/>
    <dbReference type="STRING" id="869754.A0A1A0H787"/>
    <dbReference type="GeneID" id="30031381"/>
    <dbReference type="OrthoDB" id="25921at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052400">
        <property type="entry name" value="Zn2-C6_fungal_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657:SF7">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="27"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="863"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="873"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="892" mass="102835" checksum="282764E151EDB58F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H793</accession>
    <name>A0A1A0H793_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_44454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710292.1">
        <property type="nucleotide sequence ID" value="XM_018857794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H793"/>
    <dbReference type="STRING" id="869754.A0A1A0H793"/>
    <dbReference type="GeneID" id="30030770"/>
    <dbReference type="OrthoDB" id="4138492at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="497" mass="55286" checksum="DBCC61A2075EE0D3" modified="2016-10-05" version="1">MENGLLNWVLLALLVTALLYRQFLVFTRDVPDEYLNEQSVIESIRKPNELAVHKSTKLDYSQGLRVGLGIRYSSYKLRNGNLYDIWELAMGHAKKDPTKSVFFDQKPVQIARLNSLASQVRKLLSELASEEDIDEVCIHKNLVFSDPEAFTVLLACFTSQTTVHIYDESCLDDQNGNTLDVQREEGDLVIYRGSRLVLNMTRTIDTDITNMSFVNEYSSEKDRGIALKVSCRANHMAMTSTNFVQTNLVSAVASCIKHFPPQHHISSDDRMAVLNNTASMEGLVNGLVKVLVSFVTGAELHLASSLDTCVALKPTIILAPTDVVSTMYKKPAGLDRLLKPHRLYSLACNRFSGLIQNKPHPDLRLVIAHRSIGSTSVSPDWRQISASLAAHVVEESGYFHAAGPILVSDFFDFRSFSRELSAQTRTHGAVAQSNELKLVNFDGVSGKIAARGYNFGKAKTVMDKVGETIVTPDEDGFYQLPVQGRWGLDGCMYVMRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D4</accession>
    <name>A0A1A0H7D4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Telomere replication protein EST3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_18597</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the telomerase complex involved in telomere replication. Stimulates RNA/DNA heteroduplex unwinding which favors the telomere replication by the telomerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the EST3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710332.1">
        <property type="nucleotide sequence ID" value="XM_018854799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7D4"/>
    <dbReference type="GeneID" id="30027775"/>
    <dbReference type="OrthoDB" id="4083059at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019437">
        <property type="entry name" value="TPP1/Est3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10341">
        <property type="entry name" value="TPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Shelterin complex subunit TPP1/Est3" evidence="9">
        <location>
            <begin position="8"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="21001" checksum="3813C1CDAF90648A" modified="2016-10-05" version="1" fragment="single">MAYPVTLLSSWILTTILENVERRDVYVSDIASKRFIAYHGPRNCNIKYTPLVRIFVFFPVTTKGELVAIVNDSTHKITVVFTRDSIRRFETRYGQRLTYKSVHRLIIIRQANLKFMDKNTYMDFHHVLGNIPPGSLKGTPLIYLEVNEAEYFLRDAHTVPASAESTLRFVYTAPEFQMMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7E6</accession>
    <name>A0A1A0H7E6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNI-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710425.1">
        <property type="nucleotide sequence ID" value="XM_018854282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7E6"/>
    <dbReference type="GeneID" id="30027258"/>
    <dbReference type="OrthoDB" id="4078956at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="457" mass="51977" checksum="45216A5445B5EDA3" modified="2016-10-05" version="1">MFGKRKYDRKKGVRKSFRRRRRKKGKILKLKEPEPVVEKPVFSASGSSLYLWMHVPPLHQTFVNNTERRGKFTSSLRERCAEIVGANSDALSPSYLEEAPWSCWQSVWSTILKNGSDLPELFRMFARRFGDQISFSCHASTNTDVNLVHTSLKGLRNSALNACLIPFYKKHRVENVFSNISINDFVTFVSGLDQASIAIDCSKMPPFTTSDILSLCNIPSLRALDLSGNRQVDDQLLYTIKSRLTLRESNLQILVLSGCPYVTEKGLFALLKDCGDSKLSYVEADLRLLLLESFEKLFSRIEDSNDTSIVTGTQWRLVRKDDKFAAASKYKLGLKFHFLLRQSNDLVASKMLWDIKFFSAVVDPLKENVEDNFVPAWKQRYLGAMNRPLTFPFAYMKDIDILVKPCPAPVLVQEIQSPPIFERAGRVGLQDKLKSVKQNTKKPRTISTNAQAFFFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7I8</accession>
    <name>A0A1A0H7I8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Alkaline phytoceramidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_44478</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the alkaline ceramidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710516.1">
        <property type="nucleotide sequence ID" value="XM_018857801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7I8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7I8"/>
    <dbReference type="GeneID" id="30030777"/>
    <dbReference type="OrthoDB" id="187171at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046514">
        <property type="term" value="P:ceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008901">
        <property type="entry name" value="ACER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46187:SF3">
        <property type="entry name" value="ALKALINE CERAMIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05875">
        <property type="entry name" value="Ceramidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR608901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="291" mass="33505" checksum="E654D6E418517490" modified="2016-10-05" version="1">MLPFAIPYPPEQSNGFWGAPTSTIDWCEENYVVSPYVAEALNTVTNAGFIFFAGFAIVHAWKNKLEMRFLISACGFLLVGVGSWLFHMTLRYEYQLLDELPMIYATCIPFWSVFSELRDGKGSLYVGVSTFVAANLLTWIYLKFKDPTIHQAAYGLMNAAIVFRSYRLTRDHVPNRDSAAILNRTMFTGVLIFGLGYILWNMDIHLCEYARAARREVGMPYGFLLEGHGWWHLLTGTGVYFYLVYEEYLRCWLTGTQRFYLLERKCFLPVVVLKNPTGLNAFKSARYKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K2</accession>
    <name>A0A1A0H7K2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_45139</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat PAAF1/RPN14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710397.1">
        <property type="nucleotide sequence ID" value="XM_018857883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K2"/>
    <dbReference type="GeneID" id="30030859"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF19">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT RPN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="166"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="394" mass="43031" checksum="6C9BA90D39F41942" modified="2016-10-05" version="1">MHIITIQETFLEVLLDVSSGKTLLESIWLARRDSENEEQQEFFDISVLLKDDLLVLTIGDGPEKDTYALEFPESKLHLGLRGSKISASAISPNGAILIAGTDTGHVFQYDVGSKLLAGEIKNAHLADVLRITVLPSSQVMMTVGLDLQTKLWPVTAEPEPLPVRVFSRQKAQITDIAVVDRGRNFLTASLDGSVVLWECSSAQVVSQFRRIDNLSDPVTCMALAGGETVEPGNTVGGDFLFGCQDKYMYVGYGLGTIQQYSVAGHFQTGPKLQRTSAVTSLCVEGQYLVAGYADGLLRVHDVTRSNDETHSAHWDCQMNPNFPIKQANVQRAKSGRGLVIVAANGPELLLKIEFDGARFEYSQLVGLSEMFRVVLVGSSGDAIFITTENEVVQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7L6</accession>
    <name>A0A1A0H7L6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Erg28-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_45230</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710614.1">
        <property type="nucleotide sequence ID" value="XM_018857894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7L6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7L6"/>
    <dbReference type="GeneID" id="30030870"/>
    <dbReference type="OrthoDB" id="6485510at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="144" mass="16233" checksum="145393660A07E3D9" modified="2016-10-05" version="1">MLPFVLLLPPSTAKGGYLPHWLLFISVVSVFNSLQNYLIKDLTLTRKVYSKAPRSEVTNLSARTFGTWTLLTSIVRFYGAYNLIGNSQMYNLCIWTFVVAGGHFVSEWLIFKNCKLDKGLAGPLIVASSSLVWMLNQREFYVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7M1</accession>
    <name>A0A1A0H7M1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative translation initiation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79393</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710619.1">
        <property type="nucleotide sequence ID" value="XM_018859056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7M1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7M1"/>
    <dbReference type="GeneID" id="30032032"/>
    <dbReference type="OrthoDB" id="2461at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="375" mass="41563" checksum="4D10F33EB6755017" modified="2016-10-05" version="1">MTGKTLEAIKFLKTDLLLRLLDQLKLPYTTSYIEIKSIEDAFQAIKLMQVRGAPAIAITGAFAITSETNKTIKNLVADIDYLITSRPTAVNLSNACNEIKTMLTLTFAELNTLDQKILDALFKYSVKLHEDDLRNNYKIGENGVNYIVETLRNQKFTGPFSIMTICNTGSLATSGHGTALGIIRSVYSALSRGNSSEEFWFEHAYPLETRPYNQGAKLTTYELQYEQIPFTLVCDNMATSLISLLKNGKSVKGSMAPVKFIIAGADRIVKNGDSANKIGTYQLAAIANHFNKDHPKNDREKIKFIIAAPNTNRSPYPKWGRHYAQGNVGEKFTIGVATPGIEVWNPAFDVTPCELIDSIVTEDCAIKKIDGQFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7S2</accession>
    <name>A0A1A0H7S2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710473.1">
        <property type="nucleotide sequence ID" value="XM_018859027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7S2"/>
    <dbReference type="GeneID" id="30032003"/>
    <dbReference type="OrthoDB" id="3997736at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="205" mass="23512" checksum="933F25D0E7F32619" modified="2016-10-05" version="1">MRNSLYTDNTCFPKASYFNAGPTFFNDPEAVPLQYNASKTSDSRTESTNTHEESANSLTNKRPAKELTSLIAMTALAYLALDNYQGRVRAEKLNQETTAINLKTLQIQLQNYIKARQQQDLRMLKERVEVSKRCFKMSMHIALLRKQLMELGADPKDIPDVTKEFDKHAKVSNSVQNLTGNAIWLTDESEYKGLMPDYREYDRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7V3</accession>
    <name>A0A1A0H7V3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SURF6-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_88414</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710497.1">
        <property type="nucleotide sequence ID" value="XM_018859292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7V3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7V3"/>
    <dbReference type="GeneID" id="30032267"/>
    <dbReference type="OrthoDB" id="444809at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029188">
        <property type="entry name" value="Rrp14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15459">
        <property type="entry name" value="RRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04935">
        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA-processing protein 14 N-terminal" evidence="6">
        <location>
            <begin position="8"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="5">
        <location>
            <begin position="205"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="368"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="390"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="435" mass="50296" checksum="FE643E48A8003070" modified="2016-10-05" version="1">MTNSLEERLKTHSTAFDGLLSLIPAKYYYDDATQDQWQKKKKGKKEVSEDRMAKLDPESAVTADSYNTNGASAKEVMDNRAMTAKKVSLPRKQIKNEAIKENAKEEKDIPEKSLPETKDGEELINVRSQMVFDDEGDEQASEENPAVEKTQQKSKKKELTPEEQKLREKRRADLKEKLAKKINLLREKRKAPGTKTGGPVKSREQMLTERKRREELKKQEKLKRKRDEVDDESDKSDDEEEDDEEEDEKDEDEESDVLFGNIQFADGSRMTSDLKTIRNGVDKKKKKGPANKDIKAHLTKLEAKRRKLDLLTPEERKKLEEKEQWSSLMSQAEGVKIKNDEKLLKKALKKKEKQKLKSEIEWRERKQVVKDTVSARQKRREANLKARRENKGKKSKNQQKLRKFTGVVKKTKGAKDGKKRAGFEGNAKSKGKQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H831</accession>
    <name>A0A1A0H831_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TPT-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_12303</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710781.1">
        <property type="nucleotide sequence ID" value="XM_018854207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H831"/>
    <dbReference type="STRING" id="869754.A0A1A0H831"/>
    <dbReference type="GeneID" id="30027183"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015786">
        <property type="term" value="P:UDP-glucose transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="18"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="367" mass="40593" checksum="28849A78F9AE1A1E" modified="2016-10-05" version="1">MSMSSRQEAPGQNSRLAALYIAGWYLFSVSISVYNKWMFGSGLNFRFPLFVTAFHQTCLFILSVGVLYFRPGLRPGANKASQTFRQSLAMPLKMYAAQILPCALASAGDIGLSNVALKYISLSLYTMLKTSSLVFVLLFGLLLRLERFNWRLVVIVVVMCASVMMMTEQSGKPAETTDNGSQPLGVVLILAASAVSGLRWSFTQLLLKNNEYTKHPMQTILYISPSMTAALFVFGLIFEGWQAFTALPMWVEKGVCETTLLMVAPGILAFCMTLCEFQLLSVAQILTLSVAGIFKELLTIMLGSLIFGDTLSPLNCAGLVITFLDILWYNYFRYTEPDAPAKNGYAALQQDSESQARALESIELKQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H877</accession>
    <name>A0A1A0H877_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_212641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710623.1">
        <property type="nucleotide sequence ID" value="XM_018855058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H877"/>
    <dbReference type="STRING" id="869754.A0A1A0H877"/>
    <dbReference type="GeneID" id="30028034"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052292">
        <property type="entry name" value="Glucose_repression_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28051:SF1">
        <property type="entry name" value="PROTEIN MTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="701"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="756"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="847"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="756"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="866"/>
            <end position="881"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="881" mass="97192" checksum="6849B42872F464EF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H879</accession>
    <name>A0A1A0H879_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MICOS complex subunit MIC10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_44190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Mitochondrion inner membrane</location>
            <topology evidence="2 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the MICOS complex subunit Mic10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710836.1">
        <property type="nucleotide sequence ID" value="XM_018857755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H879"/>
    <dbReference type="STRING" id="869754.A0A1A0H879"/>
    <dbReference type="GeneID" id="30030731"/>
    <dbReference type="OrthoDB" id="1916310at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007512">
        <property type="entry name" value="Mic10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21304:SF0">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04418">
        <property type="entry name" value="DUF543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="83" mass="8996" checksum="9384496BFE73953F" modified="2016-10-05" version="1">MSDQKQVATSTSQSLLNDKWDVVLSNTMIKTALGFGGGVLASVLLFKRRSFPVWLGVGFGLGRGYSEGDAIFRSDFGTRSYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8G2</accession>
    <name>A0A1A0H8G2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710797.1">
        <property type="nucleotide sequence ID" value="XM_018858925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8G2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8G2"/>
    <dbReference type="GeneID" id="30031901"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF37">
        <property type="entry name" value="ABC1 FAMILY PROTEIN C10F6.14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="143"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="549" mass="61471" checksum="7343D8B31EDD948E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8I6</accession>
    <name>A0A1A0H8I6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_198678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710951.1">
        <property type="nucleotide sequence ID" value="XM_018854905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8I6"/>
    <dbReference type="GeneID" id="30027881"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="54" mass="6670" checksum="195F141F9AB7A9D7" modified="2016-10-05" version="1">MYTMSLFSYNRWIYRYFREVISLIATLILLMDRHIILFNQSTHLLRICLLVLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8P4</accession>
    <name>A0A1A0H8P4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78851</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711011.1">
        <property type="nucleotide sequence ID" value="XM_018858959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8P4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8P4"/>
    <dbReference type="GeneID" id="30031935"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06627">
        <property type="entry name" value="STKc_Cdc7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF26">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE DDB_G0283821-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="963" mass="108889" checksum="F1120956D1E6FE71" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U8</accession>
    <name>A0A1A0H8U8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_44090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710829.1">
        <property type="nucleotide sequence ID" value="XM_018857739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U8"/>
    <dbReference type="STRING" id="869754.A0A1A0H8U8"/>
    <dbReference type="GeneID" id="30030715"/>
    <dbReference type="OrthoDB" id="3609at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000017">
        <property type="entry name" value="2-dehydropantoate 2-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051402">
        <property type="entry name" value="KPR-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708:SF30">
        <property type="entry name" value="2-DEHYDROPANTOATE 2-REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21708">
        <property type="entry name" value="PROBABLE 2-DEHYDROPANTOATE 2-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketopantoate reductase N-terminal" evidence="5">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Ketopantoate reductase C-terminal" evidence="6">
        <location>
            <begin position="191"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="333" mass="36515" checksum="357EE189C7337D29" modified="2016-10-05" version="1">MKRILLVGSGGVGTIASFTLDSCSDVEVTTVVRSDYVTVAANGYKIESVDHGNIDCYRPSKIVRSIDAASAEAPYDYVVICTKNTPDIFPLEELVAPVISTQTVVVLVQNGINIEEAFFKKFPQNVVLSGVSMISSTNYGGVISHVSHDSLKIGYFPNINLPRDLQEKVAREFVGYYQTGKNTCVYDPDVKFTRWNKLVYNATLNPICALTNVDVGRLEMFGGIDTLVRGAMKEVMAVAKADGVDLPFEVMELMIRSDDGVYYAPLMLVDIRKGNYVELEVICGNPVKIAQKLCVDAPILTIVYSLLLVVQKRLMEAKGKINVPEKRPMPAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8V1</accession>
    <name>A0A1A0H8V1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_32536</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711071.1">
        <property type="nucleotide sequence ID" value="XM_018856072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8V1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8V1"/>
    <dbReference type="GeneID" id="30029048"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903826">
        <property type="term" value="P:L-arginine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015819">
        <property type="term" value="P:lysine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018460">
        <property type="entry name" value="Battenin_disease_Cln3_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015974">
        <property type="entry name" value="CLN3_BTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="415" mass="46295" checksum="72096535F2B3BA42" modified="2016-10-05" version="1">MVLQIKRVFVSFLAFGLIKNILYVVILSAAVDIVGFSLPKAIVLLADIVPSLSVKLLAPFFIHKVPYITRIWILVVFSSFGMLLVSMSRENSTLAKLLGVSMASLSSGLGEVSFLQLTHFYDEKESIGGFSMGTGAAGILGSLSFLIMTNLAGISTRMALLVFAIIPFGFPMTYHIVLPKPQTGRNYEPLCINSTLSTIHNQETYIEDQRPPVKRLKTKAARMGNHLKNTIFSIKPLIKPYMIPLCSVYIFEYVINQGISPTLLFPLSDLPKWLITSYRDIYVVYGFVYQLGVFLSRSSLTFGFRFERLYTLSVLQMLNLLILLLQSICDFPFDNIVPVVLVVFYEGLLGGLLYVNTFLNVSQNVHPDMREFSMACVGISDSFGIMIAGFISLWLEKSLCTQQVSQGKDWCELGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H920</accession>
    <name>A0A1A0H920_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_197441</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the pacC/RIM101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710899.1">
        <property type="nucleotide sequence ID" value="XM_018854889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H920"/>
    <dbReference type="STRING" id="869754.A0A1A0H920"/>
    <dbReference type="GeneID" id="30027865"/>
    <dbReference type="OrthoDB" id="6155966at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050806">
        <property type="entry name" value="pacC/RIM101"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257:SF1">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC_RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="169"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="409"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="479"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="55738" checksum="4D12F4C5AA9930FF" modified="2016-10-05" version="1">MTTAYSLHPVSYLNATQENMAGNVLEFDLDPASDAVTNSPATQNSNSPHDSFSSQLSANSPLRHHELMKQDFSAATFVLPYGGHMVPANNASYTISGNAPGGPRQCKEESLDEVKKPKRTYNKVRAADMKGPFRCHWQLCNEIFDVPEVLYDHLCLDHVGRKSSNNLSLTCQWEGCGITTVKRDHITSHLRVHVPLKPHHCDLCTKSFKRPQDLKKHSRVHEEEYQKTLKKYQKHQSQDLSDDGSSHTMLSMHSSTNILNDFNFTGSLNSAKKQRVDAHYNTDMYNRLSSYEDTWGSGNRNAAFSAPPAPNQINFSEAEKFFNNLSSSIDAQYANFNPAPQPVYQAQAQPMYPSVPQLLNRMHMPNGFSADGYAPAPSAGFPQVHRQLGQVPQYGQNYSVYPEFGGVSNSQKTGQSLESEQAKVPKDSSSTELLDDAAAGLLSKLSLGEKFDADTVKKHRDMIKMVCEFLAARNQQAGGLEPRAKEQEAKPQAMYPEITAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H921</accession>
    <name>A0A1A0H921_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription regulator LGE1 helical region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_43406</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711022.1">
        <property type="nucleotide sequence ID" value="XM_018857653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H921"/>
    <dbReference type="STRING" id="869754.A0A1A0H921"/>
    <dbReference type="GeneID" id="30030629"/>
    <dbReference type="OrthoDB" id="4096471at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22897">
        <property type="entry name" value="Lge1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021581">
        <property type="entry name" value="Tscrpt_reg_Lge1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11488">
        <property type="entry name" value="Lge1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator LGE1 helical region" evidence="5">
        <location>
            <begin position="210"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="59"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="282" mass="31984" checksum="72C4E983D0CA570D" modified="2016-10-05" version="1">MSGNGGYYPNGESRGTGRYDSYYDKNTDSRDSRGRGGYRGGRGHGRYGANLRGRGKGFGRPDHSYGGRPLYRDANHRGGDSPRDDPRDHGPRDFAPREHPYHRDGPRDLRRDDQREILRPDSYSGRRENDDLRTGEAREHARNRDSSPGRTNGHPQLEDRGHDRADRSTERPAERLTERPQYSGMRGPSRGSNYGQKRTSDAGHGSHRTNPWITLLKLGQGKTAARVELNYKEISRVNDRLAELQSEKVKLACALSTLEVYAKRDALNVEICSEKLDEFTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H963</accession>
    <name>A0A1A0H963_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cornichon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_32424</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710940.1">
        <property type="nucleotide sequence ID" value="XM_018856046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H963"/>
    <dbReference type="STRING" id="869754.A0A1A0H963"/>
    <dbReference type="GeneID" id="30029022"/>
    <dbReference type="OrthoDB" id="434393at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033466">
        <property type="entry name" value="Cornichon_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01340">
        <property type="entry name" value="CORNICHON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="136" mass="15932" checksum="66C5628C47761654" modified="2016-10-05" version="1">MSGESWLFIFAVIVNAVNLFSQVFFTIMYSDLESDYINPIELCHKLNPWFIPEAAVHGVLTMLFIGNGFWFVFLLNLPMLAYNVNKFAKKNHLLDATEIFRTLSKHKKESFVKLGFHLLMFFFYLYRMIMALVGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H984</accession>
    <name>A0A1A0H984_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alpha-1,4 glucan phosphorylase</fullName>
            <ecNumber evidence="9">2.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_43991</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + phosphate = [(1-&gt;4)-alpha-D-glucosyl](n-1) + alpha-D-glucose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:41732"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9586"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="EC" id="2.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.1" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711201.1">
        <property type="nucleotide sequence ID" value="XM_018857730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H984"/>
    <dbReference type="STRING" id="869754.A0A1A0H984"/>
    <dbReference type="GeneID" id="30030706"/>
    <dbReference type="OrthoDB" id="9215500at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005980">
        <property type="term" value="P:glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04300">
        <property type="entry name" value="GT35_Glycogen_Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000002">
        <property type="entry name" value="Alpha-1,4 glucan phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011833">
        <property type="entry name" value="Glycg_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035090">
        <property type="entry name" value="Pyridoxal_P_attach_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02093">
        <property type="entry name" value="P_ylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11468:SF3">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE, LIVER FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000460">
        <property type="entry name" value="Pprylas_GlgP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00102">
        <property type="entry name" value="PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000460-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="892" mass="102012" checksum="AC21829AF4B93080" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H992</accession>
    <name>A0A1A0H992_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Ketol-acid reductoisomerase, mitochondrial</fullName>
            <ecNumber evidence="6">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Acetohydroxy-acid reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_32547</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711087.1">
        <property type="nucleotide sequence ID" value="XM_018856077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H992"/>
    <dbReference type="STRING" id="869754.A0A1A0H992"/>
    <dbReference type="GeneID" id="30029053"/>
    <dbReference type="OrthoDB" id="10255643at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000003">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000005">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000013">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000167">
        <property type="entry name" value="Ketol-acid reductoisomerase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016207">
        <property type="entry name" value="KetolA_reductoisomerase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000119">
        <property type="entry name" value="Ilv5_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="21" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="12 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="19">
        <location>
            <begin position="14"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="20">
        <location>
            <begin position="205"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA20565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="353" mass="39584" checksum="D512547FD394B88B" modified="2016-10-05" version="1">MAVRGVKSINFGGMQETVHERADWPREKLHEYFKDDTLALIGYGSQGYGQGLNLRDNGLNVIIGVRKNGASWKAAIEDGWVPGENLFDVNEAIGRGTYIMNLLSDAAQSETWAEIKPLITEGKTLYFSHGFSPVFKDLTHVETPENVDVILAAPKGSGRTVRSLFLEGRGINSSYAVWNDVTGKAEEKAIALAVAIGSGYVYQTTFEREVNSDLYGERGCLMGGIHGMFLAQYEVLRENGHTPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPRFKDALKPVFEDLYESVRNGSETKRSLEFNSAPDYRARLEEELVTIRNMEIWKVGKEVRKLRPENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9D3</accession>
    <name>A0A1A0H9D3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Halotolerance protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_43258</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711019.1">
        <property type="nucleotide sequence ID" value="XM_018857635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9D3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9D3"/>
    <dbReference type="GeneID" id="30030611"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045807">
        <property type="term" value="P:positive regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903329">
        <property type="term" value="P:regulation of iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13994">
        <property type="entry name" value="STKc_HAL4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000183">
        <property type="entry name" value="Serine/threonine-protein kinase hal4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF558">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 13" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="113"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="417" mass="47234" checksum="BE3E57AD5D29747C" modified="2016-10-05" version="1">MASVPAPAVSKPRFKMFENGSHEHNLRAAKRQEKLSNMLKDLLGAKKLRDEAKSAVPNILHGKNSAKGEKNAEPPTLFAGLVKQVKNNTSPYQAQTEDLIPKQDSRSFVEKYGRCQEVIGRGSFGVVRISHKKGLQNGNGEDVLYAVKEFKKRPNESEKKYSRRLTSEFCISSSLKHLNIIDTLDLLKDAKGDYCEVMEFCSGGDLYTLIIAAGKLEYAEADCFFKQLIRGVNYMHEMGVSHRDLKPENLLLTQQGVLKITDFGNGECFKMAWEDEIHFSDGVCGSSPYIAPEEFIQDQFDSRCVDIWACGVIYMAMRTGRQLWKLADPKRDEFFEEYLIQRKDATGYEPIESLKRARCRNVIYSILDPIPERRITGKQILNSEWGREIRVCAAGEAYNGVHASSEKVSEKLEKIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9M2</accession>
    <name>A0A1A0H9M2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallo-dependent phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_43176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711100.1">
        <property type="nucleotide sequence ID" value="XM_018857621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9M2"/>
    <dbReference type="STRING" id="869754.A0A1A0H9M2"/>
    <dbReference type="GeneID" id="30030597"/>
    <dbReference type="OrthoDB" id="10267127at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000298">
        <property type="term" value="F:endopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050126">
        <property type="entry name" value="Ap4A_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42850:SF4">
        <property type="entry name" value="ZINC-DEPENDENT ENDOPOLYPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="112"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="386" mass="43230" checksum="C611C9048E9A63FC" modified="2016-10-05" version="1">MSYHAVDSHEDATSFFGDSHLTKRHKHLLLAGCIVTSGIILYCLCFLLPLMFIPKPCDIHEIAQLKSLDIILNPVLSKVALPWTSFDELDHDSGHADIEYLEEITSGTKKDRLIVVGDIHGQQKELHKLLKKISFNKKSDTLLVLGDFISKGPRSLEVIDDLIELGAKCILGNHEYYVLQRYAAFHGLTAPSFERGFFKGDSTRKSMSLDTESNFNDDPEFLLAKKLQPQHVKYINLCPVMMLMGHVPLHSSHNNGKGRSGQGLAVHAGLRWDLTQSLEEQDPLGCLEMRAYIGPHFNETTDDSGKENAISWSKIWNLKHKEGISEDDYVVYYGHDAHRGLNIKRWAKGMDTGCAKGGALSAMLIWQEKSINGGLVYKENVVSVKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W3</accession>
    <name>A0A1A0H9W3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">OPT superfamily oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_11525</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711429.1">
        <property type="nucleotide sequence ID" value="XM_018854011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W3"/>
    <dbReference type="GeneID" id="30026987"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="409"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="503" mass="57989" checksum="10648A15FE1660F3" modified="2016-10-05" version="1">MDMFYGVEWADWGYQILLIFCTQFMGFGFAGIMRKFAVYPTRALWPSILPTLALNKALLQTSFLYFWLPNYLFEALSYFNWMTWKSPNNATLAVVTGSIGGLALNPLTTFDWNIIDFNGALTIPFFSQVNQYTGSILAFFCILGLWYSNYKWTGYLPINSNSLFTNTGEVYSVSSILNSDNLIDQAKYESYSPPFYTAANMVVYGAFFAIYPFTILYMGLTHWTHNKFAIGGLYSAIRNFKRSTYEGYHDAYSKSMRKYKEVPEWCFFIVLVISIVFAILPWDTISMGKLRITSQIRKWLTTCFISLAVINFNITNIKDYCDPNQSQRFTCPSARTFYSASVIWGVIGPERVFSHLYPILKWCFLIGFLLAPLMFLIKKYFSKYQAVRYFEPVLVIGGFLIYAPYNLSYFTPGVIASFFFMHVIRNRYINWFKKYVFLLSGSLNAGLAFSSIIIFFAVQYNEKYIDWWGNNVPYAGLDASSFSNLDASTAPDGYFDPRKGNYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA61</accession>
    <name>A0A1A0HA61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gfd2/YDR514C-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711400.1">
        <property type="nucleotide sequence ID" value="XM_018857460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA61"/>
    <dbReference type="STRING" id="869754.A0A1A0HA61"/>
    <dbReference type="GeneID" id="30030436"/>
    <dbReference type="OrthoDB" id="5953249at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="1">
        <location>
            <begin position="35"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="259" mass="29648" checksum="6AF92603E0A88A48" modified="2016-10-05" version="1">MNDLRSRGDRRLNTKSKLSQIQKQMDRVFTRSSALFCVDVEAWEHDTKQITEIGISIYDPSFQELALVPNIKTYHIRIAEGINFKNGDYVPDHSANFSGGDSYVFSRLQARSLLQALINFYFGPNYPFFCSLVGHDLKGDIKWLENLGVSISRNVRKLDTQTLFSYTHGRDRASLKNALRTLNQPYAFLHNAGNDAYYTILLALKLCDPGVRRLTGIEYFQPGEYPGEPVKYRKYPTNESKLTAMPVLKLFDEIIGMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA86</accession>
    <name>A0A1A0HA86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_68849</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711425.1">
        <property type="nucleotide sequence ID" value="XM_018858374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA86"/>
    <dbReference type="STRING" id="869754.A0A1A0HA86"/>
    <dbReference type="GeneID" id="30031350"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291713" description="ferric-chelate reductase (NADPH)" evidence="17">
        <location>
            <begin position="20"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="18">
        <location>
            <begin position="426"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA20915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="713" mass="82111" checksum="31FF80834E47B087" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAE5</accession>
    <name>A0A1A0HAE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711485.1">
        <property type="nucleotide sequence ID" value="XM_018858815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAE5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAE5"/>
    <dbReference type="GeneID" id="30031791"/>
    <dbReference type="OrthoDB" id="1684102at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF666">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="495"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="566"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="604"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="231"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="632" mass="69498" checksum="F3A59EF9119DAD7F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAF8</accession>
    <name>A0A1A0HAF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78097</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711506.1">
        <property type="nucleotide sequence ID" value="XM_018858819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAF8"/>
    <dbReference type="STRING" id="869754.A0A1A0HAF8"/>
    <dbReference type="GeneID" id="30031795"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000012">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000015">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000107">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF5">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="42"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="325" mass="36246" checksum="44D94F3322D5E31B" modified="2016-10-05" version="1">MPNFSLSLDLPWVEKYRPHKLDDIVGNEETIERLKLIVQDGNMPHMIILGLPGIGKTTSVHCLAHELLGPELYHLATLELNASDDRGIDVVRNKIKQFAQTKILLPPGRHKIIILDEADSMTPGAQQALRRTMELYSNTTRFAFACNQSSKIIEPLQLRCAILRYNKLADDQVLLRLLSVAKAEDVKFNSEGIKALIFTAEGDMRQAINNLQSTVAGFGFVDDINVFKIVDQPHPLVVKKILAAAIFNQSIDDAVDLLDTLWNKGYSAIDIVTLAFRVAKTLPGVSELKRLDVIKEIGFVHMRVLEGVLTYLQLCGMLAKLALLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB29</accession>
    <name>A0A1A0HB29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_41764</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711705.1">
        <property type="nucleotide sequence ID" value="XM_018857447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB29"/>
    <dbReference type="STRING" id="869754.A0A1A0HB29"/>
    <dbReference type="GeneID" id="30030423"/>
    <dbReference type="OrthoDB" id="10249309at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900036">
        <property type="term" value="P:positive regulation of cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000002">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="308" mass="34601" checksum="BC87B0E4785A41F1" modified="2016-10-05" version="1">MSDSTFDIKQTYLKFLEDDKDMTMPVAAIESLVTMLRVKQPSTSSELINLVRSSIQTLKLSISNAISLSAGCDLFMRFVLRNTKLYSDWESCKKNLVENGELFVQRAKESRDKSAAFGVPFIKDDDTILVHSYSRVVFQLLSKAKMDNLLRFKVIVTEARPSGKGYLMAKLLRDCDIPVEVIVDNAVGYMLHKVDKILVGAEGVVESGGIINHIGSYQIGCLAKANNKPFYVVTESHKFVRLFPLTPNDLPREMATILDDTNENTDDDLLKTQLIDYTPHEFITALITDLGVLTPSAVSEELIKMWYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBD5</accession>
    <name>A0A1A0HBD5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_229712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711810.1">
        <property type="nucleotide sequence ID" value="XM_018855232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBD5"/>
    <dbReference type="GeneID" id="30028208"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="226" mass="25480" checksum="38893517CF87156E" modified="2016-10-05" version="1">MTFNSTRISSDKPTGQLKANVNYARDSQALCQENNTRNSNDADKFGANIAGSFYSTQTVCSGGISDTDWKIQGKKRKKPHRNFKTKTIRTPEPAKQRSAENPIKITKPIMPSIRSGNLFSILDTIGEDEYQTFSPRRQRKDLLEATDDFVLLTATVKPGPEPDYELENFIRSRFSQTMPKSFQNHMRPSFDLDLAISMAPVLADPGIPEYTDSDQWPVTIPTQLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC99</accession>
    <name>A0A1A0HC99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_187358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712038.1">
        <property type="nucleotide sequence ID" value="XM_018854806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC99"/>
    <dbReference type="STRING" id="869754.A0A1A0HC99"/>
    <dbReference type="GeneID" id="30027782"/>
    <dbReference type="OrthoDB" id="5550281at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="165"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="165"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="76"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="279" mass="29858" checksum="A67385B2FA2DD835" modified="2016-10-05" version="1">MSTPKSAAASEESYQNAKSSLVSSLVELSTAATQTATAALNFYKYAGTNGADAATASASLKTLSETVLAAANAASMTLANGSAPEPKRTIAKPQKKKEAAPAAAPASAPAPAPAPASVSAPSSASSDVTADTTMSDVLSTTDPALLKQGLEKPKKKRAEKDPNAPKKPVTSYLRFNLAERQRLKKERGESGQPTLQATELNQIIADRWATLDDVAKLELQRAYEADYEVYKKNVEDYKTKKLTELASLHPSRLHPSQLRKNQLHLSQLHKNQHPLNQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCM8</accession>
    <name>A0A1A0HCM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_41905</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712261.1">
        <property type="nucleotide sequence ID" value="XM_018857464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCM8"/>
    <dbReference type="STRING" id="869754.A0A1A0HCM8"/>
    <dbReference type="GeneID" id="30030440"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF119">
        <property type="entry name" value="ROA1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="456"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="540"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="610"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1017"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1100"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1126"/>
            <end position="1149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1161"/>
            <end position="1180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1235"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="19"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="678"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1264" mass="140311" checksum="82E6159DF72B5B3B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCV1</accession>
    <name>A0A1A0HCV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_42079</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13501"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="Rhea" id="RHEA-COMP:10506"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:83145"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.14"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712227.1">
        <property type="nucleotide sequence ID" value="XM_018857493.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HCV1"/>
    <dbReference type="GeneID" id="30030469"/>
    <dbReference type="OrthoDB" id="14612at2759"/>
    <dbReference type="UniPathway" id="UPA00655">
        <property type="reaction ID" value="UER00711"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001295">
        <property type="term" value="P:malonyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF3">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0092">Biotin</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="19">
        <location>
            <begin position="43"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="201"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="17">
        <location>
            <begin position="679"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="20">
        <location>
            <begin position="1469"/>
            <end position="1805"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="21">
        <location>
            <begin position="1809"/>
            <end position="2124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="2154"/>
            <end position="2181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA21717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2214" mass="246602" checksum="0DE22ED0015AE37A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCW3</accession>
    <name>A0A1A0HCW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77413</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712352.1">
        <property type="nucleotide sequence ID" value="XM_018858696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCW3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCW3"/>
    <dbReference type="GeneID" id="30031672"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032984">
        <property type="term" value="P:protein-containing complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="6">
        <location>
            <begin position="49"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="278" mass="31524" checksum="06E0E5CFCD1F1CB4" modified="2016-10-05" version="1">MIRGQGSETSSTMATPSEAGSEASDTNTNPLPPLLDLGLHGYASGTHRRLMDAELAANLRSLMPARLQLYDDWHLLYSLEQDGISLNTLYRHSSPEHQRAQRQKQQNHGYAENVVKAMVLFQTPGVDAARGTRPHGYVLVIEDEKRNKFGCFLNEHLRPHDHRRYYGNGECFLWKCEWFDRHQLGPAHGLDPRRRQRFKAFVYTGVNDNVVYSNHDFIAVGLSAGNNGLWIDKSLYLGVSYPCDTFGNEILNEHGCTGHKFGKFKIINLELWRVGDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD16</accession>
    <name>A0A1A0HD16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase</fullName>
            <ecNumber evidence="3 12">2.4.1.131</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_40512</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. Catalyzes, on the cytoplasmic face of the endoplasmic reticulum, the addition of the fourth and fifth mannose residues to the dolichol-linked oligosaccharide chain, to produce Man(5)GlcNAc(2)-PP-dolichol core oligosaccharide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + 2 GDP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29523"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="EC" id="2.4.1.131"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11 12">
            <dbReference type="Rhea" id="RHEA:29524"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.131" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712366.1">
        <property type="nucleotide sequence ID" value="XM_018857283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD16"/>
    <dbReference type="STRING" id="869754.A0A1A0HD16"/>
    <dbReference type="GeneID" id="30030259"/>
    <dbReference type="OrthoDB" id="2276068at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004377">
        <property type="term" value="F:GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03806">
        <property type="entry name" value="GT4_ALG11-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038013">
        <property type="entry name" value="ALG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031814">
        <property type="entry name" value="ALG11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45919:SF1">
        <property type="entry name" value="GDP-MAN:MAN(3)GLCNAC(2)-PP-DOL ALPHA-1,2-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15924">
        <property type="entry name" value="ALG11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 12 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="233"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ALG11 mannosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="112"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="350"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF15924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="601" mass="68987" checksum="9EE368A53E0C6446" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDC2</accession>
    <name>A0A1A0HDC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712424.1">
        <property type="nucleotide sequence ID" value="XM_018853861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDC2"/>
    <dbReference type="GeneID" id="30026837"/>
    <dbReference type="OrthoDB" id="4096711at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="473" mass="51369" checksum="6A2975075CF78446" modified="2016-10-05" version="1">MGFVFNGSGFNRRTYALTVTNPRNAAAFSRAPQPLPPPPATFSQKPPVATDGKRPVPAGSSDGAEHGKRAKATHSGGTAVSRGAGGLQGAFVPAENEPRHTLPRRAVLTISSAPAHPSPPPTLVDLPPEILYRVFCFAGLGSANRLAHTCSYLHGLLDFTRNDYLLEKLVRSCLLLDLAKGTRTRDWVLRFAKRYADLPENVRGSPALEAAFHVPVRAAATTRAIDSTVFSFPGMGGHHVRFLHLRFGGIAVARRAADRQRKLKRRYLEWRYMVFKAFVALAIEDPDTPAQVLLTEAEALEQCAQYMTAHGLGGFEPLVESGAVPGHLYRAVGPASIETIRELGNTYGMRVGLPAPLVASFYRTNADVGFPGDLLDVLFLLAFEKSATDREIADALAVYCWLKDRADAGHAVPGFLHAFGRYHDFVIGMLTIYFSASADENSSGVWDRLKQAQIPELLDHVVGLGVSVPLHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDN5</accession>
    <name>A0A1A0HDN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Arginine N-methyltransferase 2</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent protein-arginine N-methyltransferase that methylates the delta-nitrogen atom of arginine residues to form N5-methylarginine (type IV) in target proteins. Monomethylates ribosomal protein L12.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RMT2 methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712690.1">
        <property type="nucleotide sequence ID" value="XM_018855777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDN5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDN5"/>
    <dbReference type="GeneID" id="30028753"/>
    <dbReference type="OrthoDB" id="19014at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019702">
        <property type="term" value="F:protein arginine N5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000310">
        <property type="entry name" value="Arginine N-methyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017408">
        <property type="entry name" value="Arginine_N-MeTrfase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051038">
        <property type="entry name" value="RMT2/GAMT_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026480">
        <property type="entry name" value="RMT2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32379:SF1">
        <property type="entry name" value="GUANIDINOACETATE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038148">
        <property type="entry name" value="Arginine_N-mtfrase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51559">
        <property type="entry name" value="SAM_RMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RMT2" evidence="7">
        <location>
            <begin position="198"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR038148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="435" mass="49690" checksum="D8861E2861392D47" modified="2016-10-05" version="1">MSDLHDLCRFAKRPIQEDYLENLKYYLKAGIPATYTIEEAFNYMNNIEGVEPSTTTTPLHLIFTHLPLDYTELELSIVKDMVTILLEYGAGWCLTDVNDETPGCILLKRNLGETILYNQIIDAGVRAELLLRKVSEFDVEIIEDTEELDHEAFANTTSTECDEETEKAAEICTILSNVAEAEKLPASFETKNSESCDPSHNQDAYLKSELQYIDGALVTKGQQDGVMMEWERDLMQLGCDSLFKGSRTESDQVDPEVNILNIGFGMGIIDEMISEKSPTKHYICEAHPDVLQKLKEEGWYDKPNVVILEGRWQEQLDKLLSSGEVYFNGIYYDTFSEHYQDMLELFDYVVGLLKPYGVFSFFNGLGADRQVIYDVYKKLVEIDLETYGLACSFQEVSVPEKTMKKGDETSVWDGIRKAYWLCPTFYHPEARFINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDV9</accession>
    <name>A0A1A0HDV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YCII-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40609</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712775.1">
        <property type="nucleotide sequence ID" value="XM_018857295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDV9"/>
    <dbReference type="GeneID" id="30030271"/>
    <dbReference type="OrthoDB" id="5519740at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1060">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII-related" evidence="1">
        <location>
            <begin position="9"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="108" mass="12449" checksum="CAC3B4A8ABA92330" modified="2016-10-05" version="1">MKVPKKLEWLVIIYDKPVNKRLAFRSDHLAKVPEKVKCGVISSCGPIFKDESKLEFIGSSFTIQAETKSEVLEFLKLDVYAEKGVWDFSNVVIHPYQPFYRSAKELPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW8</accession>
    <name>A0A1A0HDW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-B22</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase B22 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_39898</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712702.1">
        <property type="nucleotide sequence ID" value="XM_018857198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDW8"/>
    <dbReference type="GeneID" id="30030174"/>
    <dbReference type="OrthoDB" id="13598at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20263">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045292">
        <property type="entry name" value="Complex1_LYR_NDUFB9_LYRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033034">
        <property type="entry name" value="NDUFB9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12868">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="76"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="107" mass="12394" checksum="43EB742B77CE093F" modified="2016-10-05" version="1">MSKVVLPFTEANTKRVSSLFRKALRIAFDSNLKFQSYREETVRIRSQFERNKLILDPNELEVVIANTRAKLAEYTHPDPYIPPLRPGGTKYERNLPPPKEPVVPGDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDY4</accession>
    <name>A0A1A0HDY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione-specific gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.7" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712805.1">
        <property type="nucleotide sequence ID" value="XM_018855815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDY4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDY4"/>
    <dbReference type="GeneID" id="30028791"/>
    <dbReference type="OrthoDB" id="1933483at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF2">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="251" mass="27854" checksum="DD97A45C129D78D9" modified="2016-10-05" version="1">MSQNGMWIIGYGSLIFKPPPLVSFRVSGTIQGYIRRFWQSSSDHRGTPELPGRVVTLVPLDDLKQHEIFNGSVHNYEILPSSGADLALKESAQDVSKLQAHDLKVWGVAYYIEPENVEEVKQYLDIREQDGYTLHTVEFHVHSMPDNNPEANDILSALPRADDGDLYIKSHVYIGTIDNQSFVGPESIAKTAQIIKSSRGPSGDNFLYLKNLTTSVIALHSDLASADSYLEALTHLSLPASQMEPKGFSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE13</accession>
    <name>A0A1A0HE13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">lipoyl synthase</fullName>
            <ecNumber evidence="2">2.8.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_31158</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712716.1">
        <property type="nucleotide sequence ID" value="XM_018855781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE13"/>
    <dbReference type="STRING" id="869754.A0A1A0HE13"/>
    <dbReference type="GeneID" id="30028757"/>
    <dbReference type="OrthoDB" id="3231at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000036">
        <property type="entry name" value="Lipoyl synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004019">
        <property type="entry name" value="PRK05481.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009544">
        <property type="entry name" value="PRK12928.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="LIPOYL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949:SF0">
        <property type="entry name" value="LIPOYL SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="11">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="259" mass="29226" checksum="0A92004F20F9A8E1" modified="2016-10-05" version="1" fragment="single">KKSEATATIMLLGDTCTRGCRFCSVKTNRSPAKPDPMEPENTAEAILRWGLGYVVLTTVDRDDLVDGGAHHLAETVKRIKTKAPHILVEVLGGDFRGDLKSCEILASSGLDVYAHNLETVEELTPHIRDRRATYRQSLSILEKAKLTKPDLVTKTSLMLGFGETDEQIIKTLTDLREINCDVVTFGQYMRPTKRHMKVVEYVKPEKFDYWKLKALEMGFLYVASGPLVRSSYKAGEAFIENVLRKRQHNVGEAKRRTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE41</accession>
    <name>A0A1A0HE41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_77790</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712870.1">
        <property type="nucleotide sequence ID" value="XM_018858766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE41"/>
    <dbReference type="STRING" id="869754.A0A1A0HE41"/>
    <dbReference type="GeneID" id="30031742"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF317">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE ECM32-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="6">
        <location>
            <begin position="188"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="6">
        <location>
            <begin position="314"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="7">
        <location>
            <begin position="399"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="656" mass="74386" checksum="E1FD159D144110D7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE99</accession>
    <name>A0A1A0HE99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BBC1/AIM3 cysteine proteinase-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_150724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712811.1">
        <property type="nucleotide sequence ID" value="XM_018854564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE99"/>
    <dbReference type="STRING" id="869754.A0A1A0HE99"/>
    <dbReference type="GeneID" id="30027540"/>
    <dbReference type="OrthoDB" id="3357271at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057402">
        <property type="entry name" value="AIM3_BBC1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25459">
        <property type="entry name" value="AIM3_BBC1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BBC1/AIM3 cysteine proteinase-fold" evidence="2">
        <location>
            <begin position="825"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="585"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="536"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="674"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="692"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="987" mass="103930" checksum="0F27736FB60C6BB2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEI6</accession>
    <name>A0A1A0HEI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77813</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712902.1">
        <property type="nucleotide sequence ID" value="XM_018858773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEI6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEI6"/>
    <dbReference type="GeneID" id="30031749"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000131">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="838"/>
            <end position="871"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="871" mass="99829" checksum="450F4A5A54220B22" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEP0</accession>
    <name>A0A1A0HEP0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_153541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712936.1">
        <property type="nucleotide sequence ID" value="XM_018854589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEP0"/>
    <dbReference type="GeneID" id="30027565"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291772" evidence="2">
        <location>
            <begin position="18"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="192" mass="20503" checksum="1DD65082C6E22299" modified="2016-10-05" version="1">MKIFTIATLAFATLATASPVDREDWRRKARKGPSKVFHSSNAAPDALPIPRISSDSNAPKKRDIAYSSWPKFQNTSSNQSTSDQSISTGNMISLGVEESNESRGGSVSSHSISSPEPSSPPTSAVPDAAISGIIDEYQDEFGGDVYQQGAQSIDPEYAENSIDPRNEPSVSVLDSALESASLPDEERADLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HES6</accession>
    <name>A0A1A0HES6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_77805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712888.1">
        <property type="nucleotide sequence ID" value="XM_018858771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HES6"/>
    <dbReference type="STRING" id="869754.A0A1A0HES6"/>
    <dbReference type="GeneID" id="30031747"/>
    <dbReference type="OrthoDB" id="2262349at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052780">
        <property type="entry name" value="AAA_Catabolism_Regulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31644:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR ARO80-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="600"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="694" mass="78053" checksum="29478E9796A5E863" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HES8</accession>
    <name>A0A1A0HES8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712909.1">
        <property type="nucleotide sequence ID" value="XM_018853944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HES8"/>
    <dbReference type="STRING" id="869754.A0A1A0HES8"/>
    <dbReference type="GeneID" id="30026920"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="464" mass="50210" checksum="93FC294D85B0A15D" modified="2016-10-05" version="1">MVNALPPIVIDNGLSTTRAGFALEELPSLVFNTNYVVGSDGTPAFGDEAIVENAGADVMTIMDDGVVYNTDNARHNWDHVFRNLDGSNGANASEHALMATENVWTANKTRAQMAQIAFEDFQVPLFSLVKTPLAQLYQMGRSSGLVIDVGSAVASVTPILDGIIQQKSSFHSKYAGDFLSLHCLRSLEAKLSAPGTQLDYTKLMPSQFQTSALSESFKQYHVTQHLLGHFKHTMLSTAEAPPGMPASNSYYPLAPHQRPALFQLPDGTHVSYTDQELTNLTEPLFVPHAYPLAGLTLPEPAFNKAGSHGVSNLALFSIKNLEAAFLASMGSESQGANASARFNEILRQLFSSTLITGGTALVPGFADRVCGDLSRTAPQLLPGYIVTGSYKLYISPLRNHNTGSLNDIFDKKFGAWLGAANLASMLRENTETESSSMGIALDNWFVSKADYEEMGEDLIAEKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HET2</accession>
    <name>A0A1A0HET2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_152952</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712893.1">
        <property type="nucleotide sequence ID" value="XM_018854580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HET2"/>
    <dbReference type="GeneID" id="30027556"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="66" mass="7568" checksum="224DBD02B85308E8" modified="2016-10-05" version="1">MNIVSLIIFTRLGRHYGSTQGFLWKFINLWVSQVSSAEQICIVLVMCITVITLLDHCILHQYTVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF89</accession>
    <name>A0A1A0HF89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_92842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713285.1">
        <property type="nucleotide sequence ID" value="XM_018859351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF89"/>
    <dbReference type="GeneID" id="30032326"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="56" mass="6110" checksum="34A209B798EBE36C" modified="2016-10-05" version="1">MRSLSHPRICHAIHTSLITFGTMLVLVIQWGVRTAADCPVQRSSPVCIILVLTAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF97</accession>
    <name>A0A1A0HF97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH-ubiquinone oxidoreductase 9.5 kDa subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38725</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713063.1">
        <property type="nucleotide sequence ID" value="XM_018857038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF97"/>
    <dbReference type="STRING" id="869754.A0A1A0HF97"/>
    <dbReference type="GeneID" id="30030014"/>
    <dbReference type="OrthoDB" id="2093409at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039961">
        <property type="entry name" value="Nuo9.5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38488">
        <property type="entry name" value="OXIDOREDUCTASE 9.5 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G08980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38488:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 9.5 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G08980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="9841" checksum="6E9D9FD7D67AFED2" modified="2016-10-05" version="1">MGVAEKVWGDYPVYYKQPIRWVRYHAHTKPHFVFSIFFGVSAPLLLLMAPLREKYIFASHNPIPFVYPLPTRDRDATLTGFDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFH0</accession>
    <name>A0A1A0HFH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Calmodulin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_91637</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the calmodulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713227.1">
        <property type="nucleotide sequence ID" value="XM_018859327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFH0"/>
    <dbReference type="GeneID" id="30032302"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000257">
        <property type="entry name" value="Calmodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000006">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="8"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="6">
        <location>
            <begin position="117"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16578" checksum="CBD070238D737C08" modified="2016-10-05" version="1">MAEKLSEEQIAEFKEAFSLFDKDSDGKITTKELGTVMRSLGQNPSESELTDMINEVDTNSDGSIDFPEFLTMMARKMKDTDSEAEIAEAFKVFDRNGDGKISAAELRHVLTSIGEKLSDADVDQMIREADTNNDGEIDIQEFTQLLVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFK2</accession>
    <name>A0A1A0HFK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CobW-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_29355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713246.1">
        <property type="nucleotide sequence ID" value="XM_018855385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFK2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFK2"/>
    <dbReference type="GeneID" id="30028361"/>
    <dbReference type="OrthoDB" id="272672at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03112">
        <property type="entry name" value="CobW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051927">
        <property type="entry name" value="Zn_Chap_cDPG_Synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603">
        <property type="entry name" value="COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43603:SF1">
        <property type="entry name" value="ZINC-REGULATED GTPASE METALLOPROTEIN ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90002">
        <property type="entry name" value="Hypothetical protein YjiA, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="2">
        <location>
            <begin position="309"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="546" mass="61428" checksum="7DB6D62A493151C2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFN3</accession>
    <name>A0A1A0HFN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">37S ribosomal protein mrp10, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713292.1">
        <property type="nucleotide sequence ID" value="XM_018856782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFN3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFN3"/>
    <dbReference type="GeneID" id="30029758"/>
    <dbReference type="OrthoDB" id="2210at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017264">
        <property type="entry name" value="Ribosomal_mS37_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28066:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10454" checksum="DE89AAB8C1B5989F" modified="2016-10-05" version="1">MPYKKVAQPMALPKLPRLKFKKTSRAQANNPCFLLMSSLLNCWAANGEGAAQCSAFAQDLKTCMETHKPVTPPPNPMNYHAQRLYSKFKKVND</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFR9</accession>
    <name>A0A1A0HFR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phytanoyl-CoA dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37207</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PhyH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713485.1">
        <property type="nucleotide sequence ID" value="XM_018856841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFR9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFR9"/>
    <dbReference type="GeneID" id="30029817"/>
    <dbReference type="OrthoDB" id="445007at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20883:SF15">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="34086" checksum="FC0FABFF5A06E4E9" modified="2016-10-05" version="1">MTGLTQEQIKQFNEQGMLCISDFLSHAEVKQMMASAHDLLTDLDMNTHPRTQFKTGDNNHIGDKYFFESSDKVSYFFDVDAFDENGLLQYPKEQAVNKIGHGLHMHHDLFREITFKERVKDIARDLQYTDPRVLQSMLIFKNAIGKGVDEARKNDVPPHTDGTFLYTNPQSALGFWFALEDCTAANGCLSYHPGSHKSFPIQKRFVRVDGASKGCKMLELKTHVPCPQDSPEKYKLFECKAGSLILIHNSVLHKSERNVLGKSRFAYAFHLIDGVCEYDSSNWLQVPPLGGANFLRLYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG72</accession>
    <name>A0A1A0HG72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_35746</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713358.1">
        <property type="nucleotide sequence ID" value="XM_018856650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG72"/>
    <dbReference type="STRING" id="869754.A0A1A0HG72"/>
    <dbReference type="GeneID" id="30029626"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000499">
        <property type="entry name" value="Serine/threonine-protein kinase KIC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="7"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="473"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="637"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="697"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="510"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="548"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="699"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="733"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="815" mass="89885" checksum="4AEDE419059852DE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGD0</accession>
    <name>A0A1A0HGD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acyl-CoA N-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37940</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. MAK3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713418.1">
        <property type="nucleotide sequence ID" value="XM_018856932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGD0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGD0"/>
    <dbReference type="GeneID" id="30029908"/>
    <dbReference type="OrthoDB" id="249099at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044542">
        <property type="entry name" value="NAA30-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45896:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="15"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="196" mass="22328" checksum="2621E42B515D5C81" modified="2016-10-05" version="1">MLAAVDFIEDGFRFYSFDPASAPQFQAIARLIDVHLSEPYSLYVYWYFFHEWPQYCFLVSTVGSLDIVGVVISKVEPHRAVRVRGYIGMLVIDPAFRGQGLAKKLVQLSIRKMVEWDHVDEIMLETEVDNLAALHLYELFGFLRTKRMHRYYLNGSDAFRLILPVLEKLGTRIAFLAPLDTSGKPGSACAVDSARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGH6</accession>
    <name>A0A1A0HGH6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713576.1">
        <property type="nucleotide sequence ID" value="XM_018859382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGH6"/>
    <dbReference type="GeneID" id="30032357"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="366" mass="37452" checksum="8AE1101EE342B464" modified="2016-10-05" version="1">MTPVTSDVCTDGCSVTSKALMTGSDISVMSAVRSGKVTSSAVPGGGDSAAMSEITTRTDLFGPIAPGSSLPERPSADSMDSEQSPVATKTETFTHTIRSTFCFKGTCFTRVATQLHTETSTVKVTMAQTVSSTETVHVTKTKPRKESASLTKSDSMSSVSAKPSSMYISKETGSASFISVLSTSARSAAVDSILASSIPSPTISVLAESALEKMVSEMTEMTAFTLANSELSPSPASASVSVEYGSEVAVSGTTVSGIATPEVTSSPAFASGEYVSEVSASEMTSSPASASVSVEYVEYASVEHSDVTASGTTTSAVSAFTSYVSSEAASTSSSSAEPTRISIQEGSASKSVVLFILLVPFFVMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGR5</accession>
    <name>A0A1A0HGR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIH1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713553.1">
        <property type="nucleotide sequence ID" value="XM_018859374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGR5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGR5"/>
    <dbReference type="GeneID" id="30032349"/>
    <dbReference type="OrthoDB" id="5135119at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050734">
        <property type="entry name" value="PIH1/Kintoun_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012981">
        <property type="entry name" value="PIH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22997:SF0">
        <property type="entry name" value="PIH1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08190">
        <property type="entry name" value="PIH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIH1 N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="39467" checksum="CBE02B66699EBA6F" modified="2016-10-05" version="1">MAAIFEHTEAIRLSPQPGFVVKTKIVDGKGDHIYLTKVFINICHEPRVPKPSLDFQPEVVFPLIVRNEWEIPIIVSLEKRAVDKKGVPSFVYDCCINSECFQWVQVSRDLRLILVEWALESVEMMHELVLEREYALPKMLCKGELSETEITEEDLKNGLQKKLLELKQNETAGLIEELEVRPWGDGEEEGEQETLPDLTNIDGKRQKPLIEEIGQMSLDGEPKESHIFASQVSQSLNKDTHCSSSSERSVSEVLRYKFTFKRELRGNQCFVLFESRQLTKNLEVSYLKDPAGTGTVITLRNTDSLRVLESLNYLEIPMPQGYKPYQSFLLEKHGQLYIFCASEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH02</accession>
    <name>A0A1A0HH02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_9434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713609.1">
        <property type="nucleotide sequence ID" value="XM_018859388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH02"/>
    <dbReference type="GeneID" id="30032363"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="16771" checksum="09F85ED7FC271FD9" modified="2016-10-05" version="1">MALAQKVHHRLPDEISPAKHWHISSARLFINKSNTTTPIKSEAPWRITGTIERADIFERCDEACHNKYRSVTSIQGSVWVRGSLAAKYPRSGHLAIDSSRPVHHWPALARSAQEQSVLIGGHWRIAGPVAWNAALRNLRLGPVAALRPWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH29</accession>
    <name>A0A1A0HH29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29683</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713634.1">
        <property type="nucleotide sequence ID" value="XM_018855458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH29"/>
    <dbReference type="STRING" id="869754.A0A1A0HH29"/>
    <dbReference type="GeneID" id="30028434"/>
    <dbReference type="OrthoDB" id="10261918at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071021">
        <property type="term" value="C:U2-type post-spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004098">
        <property type="entry name" value="Prp18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039979">
        <property type="entry name" value="PRPF18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13007:SF19">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02840">
        <property type="entry name" value="Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47938">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Prp18" evidence="9">
        <location>
            <begin position="161"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="282" mass="31747" checksum="8E658457CC82943F" modified="2016-10-05" version="1">MDISQLLAQQLQQKQRQRQRAKQQGTPKETCPGRGDKPQGTPQAQQNEQGPEAGQPDPPVSTETDEARLLDSIDADKLAESLRRLDENGQGGLKAEQLRRLQIHVRHEKKDLRYKAYLDRETRVPAHIALADIAAQNSATLCLQIRRFFKEILQTWTLATDVRSADPVLLETKRDIVKLMYKLRSGSLPQETVVSLSTIVYYIQTENIVKANEAYLKLSIGNVAWPIGVRDVGIHARAADAKIAGDDKKQLSNIMKSEATRRWLIAVKRIVNYCAQLHDGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHM9</accession>
    <name>A0A1A0HHM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_29959</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713992.1">
        <property type="nucleotide sequence ID" value="XM_018855523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHM9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHM9"/>
    <dbReference type="GeneID" id="30028499"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140054">
        <property type="term" value="F:histone H2A reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140015">
        <property type="term" value="F:histone H3K14ac reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140008">
        <property type="term" value="F:histone H4 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17996">
        <property type="entry name" value="DEXHc_SMARCA2_SMARCA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000008">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000843">
        <property type="entry name" value="Chromatin structure-remodeling complex subunit snf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029295">
        <property type="entry name" value="SnAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14619">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01314">
        <property type="entry name" value="SnAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HSA" evidence="13">
        <location>
            <begin position="353"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="525"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="838"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="1195"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1127"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1279" mass="145769" checksum="F2172D4A286B1887" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHP4</accession>
    <name>A0A1A0HHP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Peroxin-10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_30108</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714160.1">
        <property type="nucleotide sequence ID" value="XM_018855557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHP4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHP4"/>
    <dbReference type="GeneID" id="30028533"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044721">
        <property type="term" value="P:protein import into peroxisome matrix, substrate release"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16527">
        <property type="entry name" value="RING-HC_PEX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF0">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="276"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00041230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA23679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="326" mass="37331" checksum="5007202B5A2E3C52" modified="2016-10-05" version="1">MSRANANSLPFADAATIVRANQKDNYFSSTLNTQLQDTLRIFRGQRFVNTYPEEITTIGKFLYFLVTTVFGARTLGEEYVDIQHVSRLGKRFPKLASRLSFSIYLIVVPYFVSRIARKLKSKTEGSTSWITKVLTSYSSLLDTMMNIHVALFYFEGSFYSISKRIFGLRYVFGHNKDQKKLQATGNYSVLGGIILVQFVLKMLIHLSQLSSHRKSHKASETSIGIEKQTHSSFFKLEQLEKLNKYINEEKKVSVKVNVDLSDPNQLPYIPSSSRNCMLCLSPMINPSAANCGHFFCWVCITDWMREHPECPLCRQTCLEQNLLPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHQ0</accession>
    <name>A0A1A0HHQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_9679</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713888.1">
        <property type="nucleotide sequence ID" value="XM_018859437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHQ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHQ0"/>
    <dbReference type="GeneID" id="30032412"/>
    <dbReference type="OrthoDB" id="10249382at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008275">
        <property type="term" value="C:gamma-tubulin small complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005822">
        <property type="term" value="C:inner plaque of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005824">
        <property type="term" value="C:outer plaque of spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051417">
        <property type="term" value="P:microtubule nucleation by spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000767">
        <property type="term" value="P:positive regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="71"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="482" mass="54184" checksum="938DC7F958240980" modified="2016-10-05" version="1">MPGEVVSFQLGQCGNQVGLQYWEQMALEHGIKADGTPEPFVNNQLHFESNSEVHPSDKSLVHERTDNTNVFYTYSEQNKYTPRAVLIDSEPSVIGKCMSSCPMFNPRNVQSSEGGTGAANNWRHGHLYATQHEEELTDLIDRELDKCDNLSTFQLFHSVAGGTGSGVGSFLLELLNDRYGRKKMISTFLIFPSNDKTSDVVVQPYNTVLTLKRLIDHSDATFIYDNDSLNALESLLLSPESNNPPAMTSAFERENKLIAYVACGVSNPLRFPSYMYSSYESIISTLVPTPELKFLSSSIAPYADIPGATHRKNYVSLNESDIILELLNDKCKMNHVSLHPEYISMMDYIVGDDLDQHEIRKGRIRAQSRVPFVPWTSASIHVVNGRKLTFSTSRGLSGFQISNNTSIPQLFSKIVRQYDLLAKRSAYINLYTETRDPAERTEVLETFNECKESVLSTIEEYKASTEMSYLANEFLDGDDDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI53</accession>
    <name>A0A1A0HI53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein DML1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein dml1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_35022</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the partitioning of the mitochondrial organelle and mitochondrial DNA (mtDNA) inheritance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the misato family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714004.1">
        <property type="nucleotide sequence ID" value="XM_018856557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI53"/>
    <dbReference type="STRING" id="869754.A0A1A0HI53"/>
    <dbReference type="GeneID" id="30029533"/>
    <dbReference type="OrthoDB" id="271881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049942">
        <property type="entry name" value="DML1/Misato"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029209">
        <property type="entry name" value="DML1/Misato_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019605">
        <property type="entry name" value="Misato_II_tubulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION REGULATOR MISATO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13391:SF0">
        <property type="entry name" value="PROTEIN MISATO HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10644">
        <property type="entry name" value="Misat_Tub_SegII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14881">
        <property type="entry name" value="Tubulin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Misato Segment II tubulin-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="DML1/Misato tubulin" evidence="8">
        <location>
            <begin position="117"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="500" mass="56733" checksum="FC033E792DDD95A5" modified="2016-10-05" version="1">MHEIINFSCSQASGHLITLLYNVQESHLPYTRNKTLTHTNDVFLVPNKVKGKTNYYPRSINVEFQGGYGYLGKHAYQETEISPELYDNVIVKKQERHAKNEFQANLDAGKPTHSSLLSTENTTFWTDYNKLIYRPESLVSLHGFSHPEGAHKNFPRLKFDAFNIGEEEFKVLSDEIDDFFRKNLEQLDQIQGISFFSECDTAWAGFTNEMMVFLRDEYFGNGVSSKYNLWTYGLMSPGKSQPRIDTLLTRIRAVTEFASNSTLFFPLSAPQDSESFLSSTYDSSKLWHTGAVQSMFVNAIWGLNCQVEKPVSMATIESNLLRGNSSRNIVNEISLRPDVPAKPEAEFGIVSDASMMDMYLNAGQSQTESALGSKDKNKALELGLNPESRDHLYGRGVICKHQPDLGVSDSKTDTYVNPYIDETTKIDSFPLILENHEFLTQFAQTAVLKPLLKQYREVVERVRNQRQIEILGDKAEVIESLSSLMEEYSAGYSDESDYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIA3</accession>
    <name>A0A1A0HIA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pkinase-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_36841</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714216.1">
        <property type="nucleotide sequence ID" value="XM_018856786.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HIA3"/>
    <dbReference type="GeneID" id="30029762"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000182">
        <property type="entry name" value="MAP kinase kinase kinase mkh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF48">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE BCK1_SLK1_SSP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="1004"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="58"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="669"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="770"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="675"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="788"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="1033"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1297" mass="141903" checksum="B2321D0204D70966" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIA8</accession>
    <name>A0A1A0HIA8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_38239</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 9">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714221.1">
        <property type="nucleotide sequence ID" value="XM_018856975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIA8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIA8"/>
    <dbReference type="GeneID" id="30029951"/>
    <dbReference type="OrthoDB" id="6718861at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004354">
        <property type="term" value="F:glutamate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05313">
        <property type="entry name" value="NAD_bind_2_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.285.10:FF:000001">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.285.10:FF:000003">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000002">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000030">
        <property type="entry name" value="Glutamate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.285.10">
        <property type="entry name" value="Glutamate Dehydrogenase, chain A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050724">
        <property type="entry name" value="Glu_Leu_Phe_Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006929">
        <property type="entry name" value="PRK09414.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43571:SF1">
        <property type="entry name" value="NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="189"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="8">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="451" mass="48962" checksum="A9DAFFD96F7EC2D3" modified="2016-10-05" version="1">MVLPHEPEFQQAYNELLSALEDSTLFTEHPEYKKVIPVVSVPERIIQFRVAWENDNHEVEVNNGFRVQFNSALGPYKGGLRFHPSVNLSILKFLGYEQIFKNALTGLMMGGGKGGSDFNPKNRSDNEIRRFCVAFMRQLARYIGADTDVPAGDIGVGGREVGFLFGAYKSMQNNFSGVLTGKGLSWGGSLIRPEATGYGTVYYVEKMIEKATGGKESFKGKRVAISGSGNVAQYAALKVIELGGTVVSLSDSKGSIIVQEGITPEQVQEIIVAKVKFQSLEQIISASAFSGKKVEYIAGARPWVHVGQVDIALPCATQNEVSGDEAKVLVEANCLYIAEGSNMGSTKEAIDVFEANRAKGVWYAPGKAANCGGVAVSGLEMAQNSQRVKWTEEEVDNKLKDIMYTCFENCYDTAIKYSVDKDAGLPSLLKGANIAGFIKVADAMFNQGDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HID0</accession>
    <name>A0A1A0HID0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_35104</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714237.1">
        <property type="nucleotide sequence ID" value="XM_018856573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HID0"/>
    <dbReference type="STRING" id="869754.A0A1A0HID0"/>
    <dbReference type="GeneID" id="30029549"/>
    <dbReference type="OrthoDB" id="435282at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048889">
        <property type="entry name" value="NSUN5_RCM1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21153">
        <property type="entry name" value="NSUN5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="129"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="455" mass="50935" checksum="95F991C88F8A72E0" modified="2016-10-05" version="1">MKLYFEAEKFLKGGERGLKTRIFNAKLSNSPKHIYALVISALKYKEYIQHIVRKARLKDAPELKKLKVSEALLALLVYDFLFSAKGRIQLGKHPVKDAFLANKTRMQAELTKLKLKHGVKSVAELPMKSGLDDDETPVRWIRINTIKLNAAAFFKKHPFFAELTAVDDLAQITGPGVIFKDPYIQNLYGIHPREKLTNTSAYLHGEVIIQDRASCFPGEILFNDPSDVHTQIIDATAAPGNKTTHAASFVAHSENSVVFAFERDDKRVKVLKMMCEKATGKLKKGLIHPTHADFTTVLPQDFPQVSGLIVDPSCSGSGIFGRAIEDALEQAKEDIDTERLQKLAAFQFTIMKHALSFPKARKVVYSTCSIHPHENERVVVDLLRDVAVQAQGWHLAPRDHVIALWPRRGWKEEFTSLCEGDDDRCSELAGGCVRSVPKEDGGIGFFAACFVRDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIL7</accession>
    <name>A0A1A0HIL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_10069</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714336.1">
        <property type="nucleotide sequence ID" value="XM_018853678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIL7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIL7"/>
    <dbReference type="GeneID" id="30026654"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000131">
        <property type="entry name" value="GTP-binding nuclear protein Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00627">
        <property type="entry name" value="GTPRANTC4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="215" mass="24452" checksum="C2F2817D6FC99B3D" modified="2016-10-05" version="1">MATTEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIATLGVEVHPLGFHTNYGELKFDVWDTAGQEKFGGLRDGYYINGQCGIIMFDVTSRITYKNVPNWHRDLVRVCENIPIVLCGNKVDVKERKVKARTITFHRKKNLQYYDISAKSNYNFEKPFLWLARKLVGNPQLEFVATPALAPPEVQVDSAMMQKYQQEMDQASALPLPDEDDDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIX5</accession>
    <name>A0A1A0HIX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="6">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NCS6</name>
        <name evidence="6" type="synonym">CTU1</name>
        <name evidence="10" type="ORF">METBIDRAFT_37017</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714590.1">
        <property type="nucleotide sequence ID" value="XM_018856812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIX5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIX5"/>
    <dbReference type="GeneID" id="30029788"/>
    <dbReference type="OrthoDB" id="198857at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103016">
        <property type="term" value="F:tRNA-uridine 2-sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032442">
        <property type="entry name" value="CTU1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16503">
        <property type="entry name" value="zn-ribbon_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="8">
        <location>
            <begin position="67"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Cytoplasmic tRNA 2-thiolation protein 1 C-terminal" evidence="9">
        <location>
            <begin position="347"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="398" mass="44190" checksum="B70912296F6865A0" modified="2016-10-05" version="1">MENTKVAKKDRKLKVSALCQLCHGRKAVMKRPKNLQKLCKECFFLVFETEIHNTITAAKLFYPGEAVAIGASGGKDSTVLASILKTLNERYNYGLRLVLLSIDEGIKGYRDDSLATVKRNQQQYDMDLEIVSYKELYNWLMDEIVSCAGIRSSCTYCGVLRRQALDRGAAKLGIHHVVTGHNADDMAETVLMNILRGDTARLESSCTILTQSAGSPIKRSKPFKYMYQKEIVLYAHYKKLDYFSTECTYAPEAFRGTARELLKGLGAIRPSCIMDIIYSGEHLVLSKKKKVTKKYKNGGKTKAQTKMQSGDVDEPLGNEKKLAVDQEQQINPDGSVSLGRAKTNDGNTCEKCGYITSNRICKACILLAGLEMNRAKVSIESSVDGAAKLGKTLEQLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ14</accession>
    <name>A0A1A0HJ14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_10200</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714486.1">
        <property type="nucleotide sequence ID" value="XM_018853707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ14"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ14"/>
    <dbReference type="GeneID" id="30026683"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="262"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="361"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="280"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="765" mass="83362" checksum="9D6605B4D0474A09" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ44</accession>
    <name>A0A1A0HJ44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_30275</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714388.1">
        <property type="nucleotide sequence ID" value="XM_018855595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ44"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ44"/>
    <dbReference type="GeneID" id="30028571"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="544" mass="59665" checksum="9BBC7B96750F16CB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD2</accession>
    <name>A0A1A0HJD2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30307</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714429.1">
        <property type="nucleotide sequence ID" value="XM_018855610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJD2"/>
    <dbReference type="GeneID" id="30028586"/>
    <dbReference type="OrthoDB" id="4094288at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="99" mass="10797" checksum="3B351DD6165BA16B" modified="2016-10-05" version="1">MYLFGNDQPEPKRRKLEIPLDAERFTSLGTVLRTNGTISPCQVSESDTMEICEPKTKKSIMVNSDAGSCPCGYLHGPGISHIHDITAVGFSSNMLRGPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD6</accession>
    <name>A0A1A0HJD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBM21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_118075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714592.1">
        <property type="nucleotide sequence ID" value="XM_018854095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJD6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJD6"/>
    <dbReference type="GeneID" id="30027071"/>
    <dbReference type="OrthoDB" id="1881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000164">
        <property type="term" value="C:protein phosphatase type 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001069">
        <property type="term" value="F:glycogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005979">
        <property type="term" value="P:regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2440">
        <property type="entry name" value="Carbohydrate binding type-21 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005036">
        <property type="entry name" value="CBM21_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038175">
        <property type="entry name" value="CBM21_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050782">
        <property type="entry name" value="PP1_regulatory_subunit_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307:SF36">
        <property type="entry name" value="GLYCOGEN-BINDING SUBUNIT 76A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12307">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03370">
        <property type="entry name" value="CBM_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51159">
        <property type="entry name" value="CBM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBM21" evidence="2">
        <location>
            <begin position="296"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="640" mass="68640" checksum="D15CCA0BE98AC5FB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJH0</accession>
    <name>A0A1A0HJH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Natural resistance-associated macrophage protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37134</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714641.1">
        <property type="nucleotide sequence ID" value="XM_018856825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJH0"/>
    <dbReference type="GeneID" id="30029801"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF29">
        <property type="entry name" value="IRON TRANSPORTER SMF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="501"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="531" mass="58571" checksum="E7CBC74052A2A7C0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJP9</accession>
    <name>A0A1A0HJP9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714524.1">
        <property type="nucleotide sequence ID" value="XM_018856918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJP9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJP9"/>
    <dbReference type="GeneID" id="30029894"/>
    <dbReference type="OrthoDB" id="5567124at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.100:FF:000001">
        <property type="entry name" value="40S ribosomal protein S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="82" mass="8852" checksum="608759D24E77623C" modified="2016-10-05" version="1">MVLVQDLLHPNPASEAQKHKLKTLVQSPRSFFMDVKCPGCLNITTVFSHAQTAVTCDGCSSVLCTPSGGKAKLTEGCSFRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK55</accession>
    <name>A0A1A0HK55_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_119932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714664.1">
        <property type="nucleotide sequence ID" value="XM_018854138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK55"/>
    <dbReference type="GeneID" id="30027114"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="94" mass="10589" checksum="2B7CA8EA2AE2C21B" modified="2016-10-05" version="1">MGTRFVGGLSLYGALCPLYLLCPVWIYVPLCSLRPVDLFSSFLFLSSFFSLSFLSKSPLMSLSSVCVLPAHFSGCRDWVCRRVRLAVCRQKDQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKE0</accession>
    <name>A0A1A0HKE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome b-c1 complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_133809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714952.1">
        <property type="nucleotide sequence ID" value="XM_018854349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKE0"/>
    <dbReference type="STRING" id="869754.A0A1A0HKE0"/>
    <dbReference type="GeneID" id="30027325"/>
    <dbReference type="OrthoDB" id="2391627at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019182">
        <property type="entry name" value="Cytochrome_b-c1_su10_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28254:SF1">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09796">
        <property type="entry name" value="QCR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="76" mass="8282" checksum="DE972E432C8A07C8" modified="2016-10-05" version="1">MVAYIRGPAYKVAKSGFNLSVVKAYSTSLMAWGVGGAAAVATFTDGVPLFKNTFYTKIPYFGSHWEYNPDPEDVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKP2</accession>
    <name>A0A1A0HKP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="7">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_10599</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714943.1">
        <property type="nucleotide sequence ID" value="XM_018853810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKP2"/>
    <dbReference type="STRING" id="869754.A0A1A0HKP2"/>
    <dbReference type="GeneID" id="30026786"/>
    <dbReference type="OrthoDB" id="3349449at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039756">
        <property type="entry name" value="Lsb6/PI4K2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12865">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE TYPE-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="8">
        <location>
            <begin position="207"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="624" mass="72719" checksum="AF8A83E3934BF01F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1X6</accession>
    <name>A0A1A0H1X6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="15">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_47628</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709358.1">
        <property type="nucleotide sequence ID" value="XM_018858198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1X6"/>
    <dbReference type="STRING" id="869754.A0A1A0H1X6"/>
    <dbReference type="GeneID" id="30031174"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18002">
        <property type="entry name" value="DEXQc_INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000022">
        <property type="entry name" value="Blast:Putative DNA helicase Ino80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001269">
        <property type="entry name" value="SNF2 family helicase/ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031047">
        <property type="entry name" value="DEXQc_INO80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="DBINO" evidence="19">
        <location>
            <begin position="241"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="483"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="1046"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="136"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1216"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA17963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1260" mass="143986" checksum="56A9906980BCFBB8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Z9</accession>
    <name>A0A1A0H1Z9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_13899</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709378.1">
        <property type="nucleotide sequence ID" value="XM_018854446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Z9"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Z9"/>
    <dbReference type="GeneID" id="30027422"/>
    <dbReference type="OrthoDB" id="4094394at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="21"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA17983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="334" mass="37082" checksum="D1878B34EF64EB66" modified="2016-10-05" version="1">MAIMDDSGYIFEDKLETSSYLGFSEFMVAENVHFVALNSNGDHFNGVFDNRGEFYVKNTGKSRVNVEMTGNEFLNVGVFVLNSLEAEAVPQFRVKAKASFRNFGDMYVGVSGLKPWVSIIELSSESEWYNAGMIVIRRESDSRAPLRMDAQKLVRKPFTLAPDDEVVEMPPMVNSGSICLQNAHWENTAILFGEGCIMVGSGSFFSLVLRDSADIGFHQKIIMESDSKLEVSQFQSYENEPVILVSGFGRNNEIHIDKNTDGLAYCESSGRLVLGKSNELVIAFDIGRGYDLSSFNLASQTRKSILTYSGTVPSDSRQITCKCVSKFPDTPTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H251</accession>
    <name>A0A1A0H251_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nucleotidylyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_47929</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709425.1">
        <property type="nucleotide sequence ID" value="XM_018858240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H251"/>
    <dbReference type="STRING" id="869754.A0A1A0H251"/>
    <dbReference type="GeneID" id="30031216"/>
    <dbReference type="OrthoDB" id="5591297at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02165">
        <property type="entry name" value="NMNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005248">
        <property type="entry name" value="NadD/NMNAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31285:SF0">
        <property type="entry name" value="NICOTINAMIDE MONONUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA18030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="277" mass="30772" checksum="43323499A88A1231" modified="2016-10-05" version="1">MNYKAALDVFVQSGKDFAIVYRSAKQAASVSRILVLDSSFNPPHMAHFTLAKEAIDHDFGDARAHPELSELPKMLLLLLSVKNADKVVPKPALFDHRLAMMHRMASSLESLELPVAIGLTKHAKFADKSAAIQAHCSVEPQANYASARLTFLLGFDTLIRVLDPKYYAPASLVDSLNDFMYNTDLFCLTRADTGEEFDQQQNYIGRIARGEFPDIPRLWARNIILTSVQTARESIGFISSSAIRQAYATGAVAQSPPLLKEVDEYIRSNDLYSEDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H288</accession>
    <name>A0A1A0H288_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_80147</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709463.1">
        <property type="nucleotide sequence ID" value="XM_018859180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H288"/>
    <dbReference type="STRING" id="869754.A0A1A0H288"/>
    <dbReference type="GeneID" id="30032156"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF2">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="558" mass="65661" checksum="061937C453E6A51D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4I9</accession>
    <name>A0A1A0H4I9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="14">PAGM</shortName>
            <ecNumber evidence="4 14">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 14">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_47515</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, which is a biosynthetic precursor of chitin and also supplies the amino sugars for N-linked oligosaccharides of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14 17">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 14"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709491.1">
        <property type="nucleotide sequence ID" value="XM_018858180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4I9"/>
    <dbReference type="STRING" id="869754.A0A1A0H4I9"/>
    <dbReference type="GeneID" id="30031156"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000013">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000023">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 14 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="19">
        <location>
            <begin position="58"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="19">
        <location>
            <begin position="107"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="21">
        <location>
            <begin position="206"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="20">
        <location>
            <begin position="299"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="18">
        <location>
            <begin position="455"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="15">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="17">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="375"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="500"/>
            <end position="504"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="509"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA18956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="532" mass="58918" checksum="610D7B69FB94939C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4P6</accession>
    <name>A0A1A0H4P6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GRF-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33667</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709584.1">
        <property type="nucleotide sequence ID" value="XM_018856372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4P6"/>
    <dbReference type="STRING" id="869754.A0A1A0H4P6"/>
    <dbReference type="GeneID" id="30029348"/>
    <dbReference type="OrthoDB" id="545910at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="entry name" value="Alpha-ketoglutarate-dependent dioxygenase AlkB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032854">
        <property type="entry name" value="ALKBH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31212:SF4">
        <property type="entry name" value="ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="6">
        <location>
            <begin position="342"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="423" mass="48677" checksum="56C3EC8A9F3B9A34" modified="2016-10-05" version="1">MDDLFEDKLLLLRLYYPAVAQEILKDFLTNCDGDLDATRSLIDGKPRPKKRQSSAQAALTKFTQKRPKPNSKNVFESDATSHSPKTAPAAHSGDRQNVRNVLPIITLNTPLDVDEHLSPYASLHLNFLPESISNRLVHDLVEKQEAFRSNKFHLFGNQCELNSSTGVFARPAAPYSQLVFNGLKMPKPFPYSKSMEEVSECVETFLNEKVIPQSQRLKWQLNEPWTNDFSVVNCYEKLQNNMDWHSDRLSHIGPHNYVASISLGTTRMFRLRNTYKERAPIYQIPLPHNSLFIMRPGCQEEFKHCVSPMLKAIDVHPVLGTTRFGITARCYTTFLIENLPRCKCDTKMVLRRSHKSLVNRGKYFWLCENVYQNKGCNSFYWCDFTNVEGNYTAKDDSNISIWIAPEDTDKLNYDKNFSINTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Y4</accession>
    <name>A0A1A0H4Y4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0103-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709671.1">
        <property type="nucleotide sequence ID" value="XM_018858063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Y4"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Y4"/>
    <dbReference type="GeneID" id="30031039"/>
    <dbReference type="OrthoDB" id="417112at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04336">
        <property type="entry name" value="AmmeMemoSam_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060:SF0">
        <property type="entry name" value="PROTEIN MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="315" mass="34360" checksum="DA58A73D3A7801CF" modified="2016-10-05" version="1">MLTRPATHSGSWYSKDRTVLSEQVARFLAQAPPPKPGARVLVGPHAGYAYCGQRLAETYKAWDCRHTQTVFILGPSHHVFFQDTALLTAHAGYSTPLGHVPVATGICEALVGQGLAFGYMSGSVDADEHLFEMHLPFLVQRCLDENIPVPRVVPIMVSGLSAHARGVLVAALRPYFADPHNTFVVLSDFCHWGRRFGYTKYVSDPGLLRPTAYEKAPAHKVPIYKSIEYLDRSAMQVAAAGLLAEWDAYIETTGNTICGQRPIAVVLLLLEDYRARGGELASGAVFQWLGYSQSSRATSASDSSVSYAAGYVQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H542</accession>
    <name>A0A1A0H542_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ARF GTPase-activating protein GIT1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_21702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709605.1">
        <property type="nucleotide sequence ID" value="XM_018855094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H542"/>
    <dbReference type="STRING" id="869754.A0A1A0H542"/>
    <dbReference type="GeneID" id="30028070"/>
    <dbReference type="OrthoDB" id="5588096at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005826">
        <property type="term" value="C:actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902716">
        <property type="term" value="C:cell cortex of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036267">
        <property type="term" value="P:invasive filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039892">
        <property type="entry name" value="Spa2/Sph1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601:SF0">
        <property type="entry name" value="PROTEIN SPA2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21601">
        <property type="entry name" value="SPA2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ARF GTPase-activating protein GIT1 C-terminal" evidence="1">
        <location>
            <begin position="53"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="194" mass="22383" checksum="5AA2F9952E3A1444" modified="2016-10-05" version="1" fragment="single">EEFEDADDDFFDEEEDEIRQRQDYRKSMAAATFNFDLFDIDDPDNTVTQVLLYLEHQTVQVISTIQDLLSAIKKPDATRGELRENSGAISEVIKQMTEATNTSMNQTRNYLLREHGSWVVKSLEDCNHRMNALCKPNTEKQDVEFADRHFKQRLAGISFDIAKCTKELVKTVEEASLKEDIAHLDARLNHPDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H553</accession>
    <name>A0A1A0H553_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Calcipressin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RCAN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709572.1">
        <property type="nucleotide sequence ID" value="XM_018858157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H553"/>
    <dbReference type="STRING" id="869754.A0A1A0H553"/>
    <dbReference type="GeneID" id="30031133"/>
    <dbReference type="OrthoDB" id="17212at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006931">
        <property type="entry name" value="Calcipressin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04847">
        <property type="entry name" value="Calcipressin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26284" checksum="580BE280444B97C8" modified="2016-10-05" version="1">MPRSPTNSLILTNLDASLEGNPEILARDLSYGNFNVELVSLPRFGRIIIICMSAADATSVRDYLTEKLEIKALMSFSIRDNNLRLLDDSLWAVETNDVHFLELPLEDGSRRFLILPPLSPHSEWNDFDKAEEGPNKKAVYSPNELTHLLWDRLGGFASSHVRKYQYDENASDHESDLTEIRGCEDTVFDLENKPEILFQDIENGAPAIVIDRVNNQEKAGPGVLPKTAIPPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H576</accession>
    <name>A0A1A0H576_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-xylose reductase [NAD(P)H]</fullName>
            <ecNumber evidence="3">1.1.1.307</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_33438</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>xylitol + NAD(+) = D-xylose + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17151"/>
            <dbReference type="ChEBI" id="CHEBI:53455"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.307"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>xylitol + NADP(+) = D-xylose + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27445"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17151"/>
            <dbReference type="ChEBI" id="CHEBI:53455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.307"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; D-xylose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.307" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709766.1">
        <property type="nucleotide sequence ID" value="XM_018856295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H576"/>
    <dbReference type="STRING" id="869754.A0A1A0H576"/>
    <dbReference type="GeneID" id="30029271"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="UniPathway" id="UPA00810"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032866">
        <property type="term" value="F:D-xylose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019568">
        <property type="term" value="P:arabinose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071470">
        <property type="term" value="P:cellular response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042843">
        <property type="term" value="P:D-xylose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019388">
        <property type="term" value="P:galactose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19113">
        <property type="entry name" value="AKR_AKR2B1-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000007">
        <property type="entry name" value="NAD(P)H-dependent D-xylose reductase xyl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044486">
        <property type="entry name" value="AKR2B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0859">Xylose metabolism</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="16"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="318" mass="36074" checksum="07D6CDFE2A69960E" modified="2016-10-05" version="1">MSTIKLNSGYEMPQVGFGCWKVTNDTCADTIYNAIKVGYRLFDGAQDYGNEKEVGQGLNRAIDEGLVARDELFVVSKLWNNFHHPDNVEKALDKTLGDLNVEYLDLFLIHFPIAFKFVPFEEKYPPGFYCGDGDKFHYEDVPLLDTWRALEKMVKKGKIRSIGISNFSGALIQDLLRGAEIAPAVLQIEHHPYLQQPRLVEYVKSKGIAITAYSSFGPQSFIELDHPKVKECVTLFDHDTILSVARAHNKSAGQVLLRWATQRGLAVIPKSNKTERLVQNLEVNDFDLSDAELKSISKLDVGLRFNNPWDWDKIPIFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F4</accession>
    <name>A0A1A0H5F4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_219325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709669.1">
        <property type="nucleotide sequence ID" value="XM_018855118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F4"/>
    <dbReference type="GeneID" id="30028094"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="58" mass="6501" checksum="B22522AE22B82328" modified="2016-10-05" version="1">MRNVNKKRGGTRKKQIQQGFLLVLQEVGLGQPALRTALPLEKTGAHAIIYLTHCKHHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5I3</accession>
    <name>A0A1A0H5I3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_33414</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the anion exchanger (TC 2.A.31) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709737.1">
        <property type="nucleotide sequence ID" value="XM_018856288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5I3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5I3"/>
    <dbReference type="GeneID" id="30029264"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080139">
        <property type="term" value="F:borate efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.570:FF:000003">
        <property type="entry name" value="Anion exchange family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.570">
        <property type="entry name" value="Helical hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF82">
        <property type="entry name" value="BORON TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="9"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="8">
        <location>
            <begin position="204"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="545" mass="61074" checksum="E0C23740AAD0994E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5P2</accession>
    <name>A0A1A0H5P2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Actin-related protein 2/3 complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_46419</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as a component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Arp2/3 complex plays a critical role in the control of cell morphogenesis via the modulation of cell polarity development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ARPC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709765.1">
        <property type="nucleotide sequence ID" value="XM_018858037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5P2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5P2"/>
    <dbReference type="GeneID" id="30031013"/>
    <dbReference type="OrthoDB" id="429520at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.190:FF:000003">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.190">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006789">
        <property type="entry name" value="ARPC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036743">
        <property type="entry name" value="ARPC5_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12644">
        <property type="entry name" value="ARP2/3 COMPLEX 16 KD SUBUNIT P16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04699">
        <property type="entry name" value="P16-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039096">
        <property type="entry name" value="p16-ARC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69103">
        <property type="entry name" value="Arp2/3 complex 16 kDa subunit ARPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00060329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="155" mass="17520" checksum="01414CAC3F981E7C" modified="2016-10-05" version="1">MEDWRRIDIDALEPENHLSKQDLVPDLPPVTPSEIAAIAQQVKQALSLGQFLQALQAVLETPPYVSDEKTKNVHTETVFEVLVSIKNNHNTHEFSSFVSQLSSDQQDTLVKYIYKIMSTSYGAKHGALLLSWFEKTVEVTGMGPVVRFYSDRRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5R7</accession>
    <name>A0A1A0H5R7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_221486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709832.1">
        <property type="nucleotide sequence ID" value="XM_018855144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5R7"/>
    <dbReference type="GeneID" id="30028120"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="172" mass="19014" checksum="D38AF720DD1C8841" modified="2016-10-05" version="1">MDITANRTDITGNRTDIMASRTDITNNLADITYNRMDITGNRTDTASNRTDITSNETDITSNRTDIPANRTGSPANRPERYLQAFKKKKHFHKCCNAAHNPIHTCVPRGVVTPSPRAGVCLSCSILRGESSRPSLLTGAQCWRNAHSGYYPSWSNISKPRKPRKPAAIGKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5S3</accession>
    <name>A0A1A0H5S3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_13563</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709795.1">
        <property type="nucleotide sequence ID" value="XM_018854390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5S3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5S3"/>
    <dbReference type="GeneID" id="30027366"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000490">
        <property type="entry name" value="CRM1p Major karyopherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="33"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1081" mass="123713" checksum="C4FBFB19D59F1DF5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6I0</accession>
    <name>A0A1A0H6I0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710046.1">
        <property type="nucleotide sequence ID" value="XM_018856241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6I0"/>
    <dbReference type="GeneID" id="30029217"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="131" mass="14872" checksum="1AC03A394724CEDA" modified="2016-10-05" version="1">MKFLNPITFFTLMLPTTVMGAGILLLESLALKVLKLEVNGERIEASITGTFFNMFESKAEYEGKYVGYKDGVLVMTDSNEDVLHFVFNEERDAFDLEYQPGELFEIANNNLIYPLGGYEDGETFRIVYIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7C9</accession>
    <name>A0A1A0H7C9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710456.1">
        <property type="nucleotide sequence ID" value="XM_018854287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7C9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7C9"/>
    <dbReference type="GeneID" id="30027263"/>
    <dbReference type="OrthoDB" id="2430277at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22064">
        <property type="entry name" value="WH2_WAS_WASL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003124">
        <property type="entry name" value="WH2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02205">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00246">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51082">
        <property type="entry name" value="WH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WH2" evidence="2">
        <location>
            <begin position="70"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="380"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="191"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="349"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="442"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="628" mass="63188" checksum="20B34965F8F5FB07" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7J9</accession>
    <name>A0A1A0H7J9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_45036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710480.1">
        <property type="nucleotide sequence ID" value="XM_018857864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7J9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7J9"/>
    <dbReference type="GeneID" id="30030840"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013888">
        <property type="entry name" value="RNase_P_Rpm2_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08579">
        <property type="entry name" value="RPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1241" mass="142969" checksum="ACE48A748E103CA5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K1</accession>
    <name>A0A1A0H7K1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_25869</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710599.1">
        <property type="nucleotide sequence ID" value="XM_018855303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K1"/>
    <dbReference type="GeneID" id="30028279"/>
    <dbReference type="OrthoDB" id="5555409at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="entry name" value="Ribosomal protein L9, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_bL9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_bL9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_bL9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_bL9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="L9 N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein L9" evidence="4">
        <location>
            <begin position="16"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="176" mass="18693" checksum="F7728B0F309A7FC6" modified="2016-10-05" version="1" fragment="single">IRWASTKTAAKSGKVHVQLLQDCLPLGVKGQIVRVKPGYMRNFLHVGNKACYIVDGQGPRIPVVEKPRPASPAEGKKVVLKKPVKKESVPVLSLDDLSNLFTNARTAKAAEDAPAQSFTAEPATSTYSLAELGDSLPGTFTISAPSFPISKDFLSATVFNSTGIEIPSSAITVKDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7M3</accession>
    <name>A0A1A0H7M3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_72615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710417.1">
        <property type="nucleotide sequence ID" value="XM_018858406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7M3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7M3"/>
    <dbReference type="GeneID" id="30031382"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052400">
        <property type="entry name" value="Zn2-C6_fungal_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657:SF7">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="723" mass="82445" checksum="692A7C3BA8108CB8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7M6</accession>
    <name>A0A1A0H7M6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710624.1">
        <property type="nucleotide sequence ID" value="XM_018859058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7M6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7M6"/>
    <dbReference type="GeneID" id="30032034"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052942">
        <property type="entry name" value="LPS_cholinephosphotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43404">
        <property type="entry name" value="LIPOPOLYSACCHARIDE CHOLINEPHOSPHOTRANSFERASE LICD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43404:SF1">
        <property type="entry name" value="MNN4P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="1">
        <location>
            <begin position="303"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="582" mass="67555" checksum="50DBD739C0BDE7F0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Q5</accession>
    <name>A0A1A0H7Q5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">Proteasome assembly chaperone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_79361</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in 20S proteasome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the 20S proteasome chaperone.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the PSMG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710581.1">
        <property type="nucleotide sequence ID" value="XM_018859046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Q5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Q5"/>
    <dbReference type="GeneID" id="30032022"/>
    <dbReference type="OrthoDB" id="10260712at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016562">
        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12970:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010044">
        <property type="entry name" value="UCP010044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="238" mass="26022" checksum="D146DC5AF22CF7FC" modified="2016-10-05" version="1">MTLRNSILLIPSVSIANIPQLALDLLLHSLEFKKVATLSDDYLYPFVSPVDHASTSQPPGGVSFGLEVFFCKGQNLTLIQQRAPLMPGFPARHVAEVIVPFVEASQFKHVVVLHLLDAGLARGVLPGDVLIFTSEDVLTESFSSLSISKTATDPTAVIAQDTSPYAKALVDIISKYSTLSLIVIYAYEGDNLYDAQVMGRKSADFLLLRVEEWRSPVSWYGVYGDKPVPLAMEEGMYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Q7</accession>
    <name>A0A1A0H7Q7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_44964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710458.1">
        <property type="nucleotide sequence ID" value="XM_018857856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Q7"/>
    <dbReference type="GeneID" id="30030832"/>
    <dbReference type="OrthoDB" id="4092812at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16741" checksum="3FFF4788E8952259" modified="2016-10-05" version="1">MFLFIIVVAVVLCVIILYLPYAAGLTTVEKQARKTSEQEKASQTANQYSGYLPPDEELKLQQEAASKGQTSALKNKMKVTSESLPIHIKLNQDGVLRKRTERSVGDLNPNNYDYDLDELIKEETEGEAQRKAQEFYAKQKLGGDKEAMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U0</accession>
    <name>A0A1A0H7U0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosomal protein S5 domain 2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_212004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710575.1">
        <property type="nucleotide sequence ID" value="XM_018855049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7U0"/>
    <dbReference type="GeneID" id="30028025"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="entry name" value="Impact, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301:SF17">
        <property type="entry name" value="IMPACT FAMILY MEMBER YDL177C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="IMPACT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="Impact_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Impact N-terminal" evidence="2">
        <location>
            <begin position="39"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="19798" checksum="A9F4525C44ED361B" modified="2016-10-05" version="1">MKMSLLLPRAHLSSIYTIFGRRNYSIVKNWNESQIITDRRSKFQARHVEISDLTEIPEILRQFLADHKSIAKNSSHPHMLAWRTGQLSSADPGQDPVYTNIQQGFKDCGEKGAGSKILDVLISQNAMNKLVIVTRWFGGSHLGSLRFRHIVNCSAESLRKGSHGEQRCLKRKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Y9</accession>
    <name>A0A1A0H7Y9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_44740</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710625.1">
        <property type="nucleotide sequence ID" value="XM_018857832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Y9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Y9"/>
    <dbReference type="GeneID" id="30030808"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="17"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="365"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="383"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="712" mass="81023" checksum="A25A1C691ECEC225" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H834</accession>
    <name>A0A1A0H834_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Redoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_43110</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710786.1">
        <property type="nucleotide sequence ID" value="XM_018857618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H834"/>
    <dbReference type="STRING" id="869754.A0A1A0H834"/>
    <dbReference type="GeneID" id="30030594"/>
    <dbReference type="OrthoDB" id="195498at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF16">
        <property type="entry name" value="PEROXIREDOXIN-5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Redoxin" evidence="7">
        <location>
            <begin position="47"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="190" mass="20298" checksum="983B125B09A487C5" modified="2016-10-05" version="1">MAISSGSNFPSGVDFAYIPIDLSTIKNEDALGCSKPLPLKLDKLIEQNAGSNILFVSVPGAFTPTCTENHVPPYLDHLEQLKKEKNISAVVVLSANDPFVLNAWGKLLLKNAKFDVGADLPKVLFASDPNAQFSKDNEISLDLTGNGMGVRTGRYAFVVNADTRSVNYYGVESGSGVEASGYEAIKAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H839</accession>
    <name>A0A1A0H839_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RWD-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_19372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710791.1">
        <property type="nucleotide sequence ID" value="XM_018854859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H839"/>
    <dbReference type="STRING" id="869754.A0A1A0H839"/>
    <dbReference type="GeneID" id="30027835"/>
    <dbReference type="OrthoDB" id="277175at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031333">
        <property type="term" value="P:negative regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040213">
        <property type="entry name" value="GIR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12292">
        <property type="entry name" value="RWD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="1">
        <location>
            <begin position="9"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="223" mass="25792" checksum="81FF4AC8768FD519" modified="2016-10-05" version="1" fragment="single">MDPHEEQEQEIEVLRSIYPDELTVLSPTHFTIYLNLETSSDRKHALLLDVKYPPSYPEVAPGLDIRTVQVEDDSADYEDLDDDDNDAPEQYVSLAEGVEFERPDLAELLDKINEEAEMNIGIPSIFAVAAILKDEAELLFQRKVDQAQKEYDDELLAREAVEQKKFNGTKVTQESFTQWRNEFRAEMKVEARDKERFDRMHNGKMTGREIFEKGLAGEFDDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H843</accession>
    <name>A0A1A0H843_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710587.1">
        <property type="nucleotide sequence ID" value="XM_018854301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H843"/>
    <dbReference type="GeneID" id="30027277"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="226" mass="24575" checksum="AF5A85D2A9D064DC" modified="2016-10-05" version="1">MPRPKSALDIAARPNSPRAQPSTGPPDNTSPHWKQSSKNRYLQKDAVMHAVVPSSGEQRATIALRPAELRDPGPGVWATANNMCGSMVVGPASGPNFKQDTAAWRGMAHISSVSFVSLSRPRDIEKENGGNRGPGGELLAHDKWGKGECGNERLPDAWVFWSWSIFFFSTVHILQIGVSLSWGQGHGAGKLWWPKPKSRASSVGWKSLIRPVQNLISPAVASHYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H896</accession>
    <name>A0A1A0H896_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710768.1">
        <property type="nucleotide sequence ID" value="XM_018854205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H896"/>
    <dbReference type="GeneID" id="30027181"/>
    <dbReference type="OrthoDB" id="10307470at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="577" mass="64973" checksum="31A8D0E1B2BCF014" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8P0</accession>
    <name>A0A1A0H8P0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_199838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711006.1">
        <property type="nucleotide sequence ID" value="XM_018854918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8P0"/>
    <dbReference type="GeneID" id="30027894"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="172" mass="19678" checksum="4E7EEFB67EBE26BD" modified="2016-10-05" version="1">MSRLSNILVQGNFCALERVLYCSELRASSPRRSQDIAHSLYAYGLGEGIKQLPTMNIWMHGLHTPHGCWLSIRWAWNLFSQFWHIFFGRPSVRARGCSRRPLPQSARIEATRRGLGRAPGLRRPEADFIVRVHGNLNDAQLIKHYIKEFLIPNCGAMLNDEKIAITNIKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8T1</accession>
    <name>A0A1A0H8T1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710815.1">
        <property type="nucleotide sequence ID" value="XM_018856024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8T1"/>
    <dbReference type="GeneID" id="30029000"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="78" mass="8427" checksum="4A95A2DE9EC17903" modified="2016-10-05" version="1">MRSGWNLGHQKIKATVCISAGATVCMVQSHGKISETNKVWGGESKTEARAIFEFRGFMEIGAVSADPRPEPGKSKEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8T2</accession>
    <name>A0A1A0H8T2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_32309</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710814.1">
        <property type="nucleotide sequence ID" value="XM_018856023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8T2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8T2"/>
    <dbReference type="GeneID" id="30028999"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04323">
        <property type="entry name" value="AsnRS_cyto_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000040">
        <property type="entry name" value="Asparagine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1910.20">
        <property type="entry name" value="asparaginyl-tRNA synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048952">
        <property type="entry name" value="AsnRS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20917">
        <property type="entry name" value="AsnRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="248"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA20292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="61501" checksum="D354296FD2076907" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9B9</accession>
    <name>A0A1A0H9B9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_43675</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711004.1">
        <property type="nucleotide sequence ID" value="XM_018857685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9B9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9B9"/>
    <dbReference type="GeneID" id="30030661"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="485" mass="54895" checksum="45FE3607D6D22ACA" modified="2016-10-05" version="1">MAQAESSARKEVTASVQPDQQQKTPPELPAFTVKNVALDIVNGAVVLVYEPAVNRAVVPVLVFLSSMICKFIISHVPYTEVDFSTYMQQVELVNAGALDYSQIVGDTGPVVYPAGFIQVYQTLYWLTDGGANLRIAQLAFGYLFSVTVIFTCAVYTMAGDFPPWPLYLLLCSKRLYSIYVLRMFNDCFTTIGVLAVILILQQASYWAPRLSGTVTFWLCAVAADVFSMALSVKMNVLLYLPAFVIVVYFLVGERLFHCLAVVAVIPLVQVLIGWRFLLPLFWDDEARYLRRMYLTRAFDFSRKFLHTWTVNWRFVPEDMFVSDEFARYLLVGHVAVLLLFVATRFVSPRVTQKSLWHLAKDGLFRPFKNTRASGNLFIQQSSGPRLILLTFAVTNLVGILFSRSLHYQFLSWYQWLFPFMLHAAGCNVFTGALIFAAHEWCWNVFPSTVDSSKLLVAILAVVLVATWYNADYYLGGKPKALETKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9T7</accession>
    <name>A0A1A0H9T7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_205630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711282.1">
        <property type="nucleotide sequence ID" value="XM_018854977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9T7"/>
    <dbReference type="GeneID" id="30027953"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="56" mass="6390" checksum="388369BB5E70827C" modified="2016-10-05" version="1">MVGMPRYRAKLAFQWAWLWVGLVVGGILVSRHGGRRAYTQSRQGLGWRFSPTPSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W1</accession>
    <name>A0A1A0H9W1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_205822</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711307.1">
        <property type="nucleotide sequence ID" value="XM_018854982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9W1"/>
    <dbReference type="GeneID" id="30027958"/>
    <dbReference type="OrthoDB" id="289314at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06867">
        <property type="entry name" value="PX_SNX41_42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044106">
        <property type="entry name" value="PX_Snx41/Atg20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051079">
        <property type="entry name" value="Sorting_Nexin_Autophagy"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46979:SF2">
        <property type="entry name" value="SORTING NEXIN-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="10">
        <location>
            <begin position="74"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="11"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="512"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="609" mass="70470" checksum="B94DAD47855094D9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9X1</accession>
    <name>A0A1A0H9X1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_42549</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711439.1">
        <property type="nucleotide sequence ID" value="XM_018857555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9X1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9X1"/>
    <dbReference type="GeneID" id="30030531"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44464">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44464:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="551"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="660" mass="71639" checksum="BB6A7ED5FFE2295D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA21</accession>
    <name>A0A1A0HA21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_41027</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711489.1">
        <property type="nucleotide sequence ID" value="XM_018857345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA21"/>
    <dbReference type="STRING" id="869754.A0A1A0HA21"/>
    <dbReference type="GeneID" id="30030321"/>
    <dbReference type="OrthoDB" id="338231at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11711">
        <property type="entry name" value="GINS_A_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21692">
        <property type="entry name" value="GINS_B_Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031633">
        <property type="entry name" value="SLD5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038749">
        <property type="entry name" value="Sld5_GINS_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16922">
        <property type="entry name" value="SLD5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007764">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GINS subunit" evidence="8">
        <location>
            <begin position="81"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="DNA replication complex GINS protein SLD5 C-terminal" evidence="9">
        <location>
            <begin position="181"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="233" mass="26892" checksum="9D6F68668E31FAB4" modified="2016-10-05" version="1">MEIDDILADFEKDTRGARTESSAGLRQQLVTAMLNERMAPDLLPYKHTLMARILRGILDQQQFLLDSYEYGDSNAEAGVISLDFKLQLMIVETDIERLSYLVRLYIRTRLAKIDEFTIYYINKTAEELPGEPLLSEPEQSYMHGHFKILNQLYNNSFLKKFPSFLTLLDDTTGGENMITTPDLDRPVFIRVVCDDTIVLHLGNDELELVKDGIYVVRYRLIMKYLELGDVVLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA33</accession>
    <name>A0A1A0HA33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Calcium permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_31630</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711499.1">
        <property type="nucleotide sequence ID" value="XM_018855889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA33"/>
    <dbReference type="STRING" id="869754.A0A1A0HA33"/>
    <dbReference type="GeneID" id="30028865"/>
    <dbReference type="OrthoDB" id="16982at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015369">
        <property type="term" value="F:calcium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015386">
        <property type="term" value="F:potassium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1420.30:FF:000014">
        <property type="entry name" value="Cation/H+ exchanger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004713">
        <property type="entry name" value="CaH_exchang"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005185">
        <property type="entry name" value="YccF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503">
        <property type="entry name" value="VACUOLAR CALCIUM ION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31503:SF10">
        <property type="entry name" value="VNX1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03733">
        <property type="entry name" value="YccF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="245"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="424"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="535"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="565"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="601"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="835"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="872"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="904"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="935"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="963"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="domain" description="Inner membrane component" evidence="12">
        <location>
            <begin position="243"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="536"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="11">
        <location>
            <begin position="834"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="764"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="34"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="367"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1009" mass="112972" checksum="CFBD1388440928AA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA82</accession>
    <name>A0A1A0HA82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711305.1">
        <property type="nucleotide sequence ID" value="XM_018854261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA82"/>
    <dbReference type="GeneID" id="30027237"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291889" evidence="1">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="129" mass="13829" checksum="A7C793A5EDE2013D" modified="2016-10-05" version="1">MKIFSVLPLLSLAFASTPAEDGEVALTICDLHSNSKDIYVYIKSDGSVTESVGALLYELKNNLVFEGGRYLVLTNNKLAMAYESDHSVYIYETTLRHGSGPVFELCGDGLIGVESGCAGAESILISTNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA83</accession>
    <name>A0A1A0HA83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase</fullName>
            <ecNumber evidence="3 14">2.7.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_78130</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Bifunctional inositol kinase that acts in concert with the IP6K kinases to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, may regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, and exocytosis. Phosphorylates inositol hexakisphosphate (InsP6).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>1D-myo-inositol hexakisphosphate + ATP = 1-diphospho-1D-myo-inositol 2,3,4,5,6-pentakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:37459"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58130"/>
            <dbReference type="ChEBI" id="CHEBI:74946"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:37460"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate + ATP + H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:10276"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58628"/>
            <dbReference type="ChEBI" id="CHEBI:77983"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10277"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
            <location evidence="1 14">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the histidine acid phosphatase family. VIP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.24" evidence="3 14"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711549.1">
        <property type="nucleotide sequence ID" value="XM_018858828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA83"/>
    <dbReference type="STRING" id="869754.A0A1A0HA83"/>
    <dbReference type="GeneID" id="30031804"/>
    <dbReference type="OrthoDB" id="18042at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033857">
        <property type="term" value="F:5-diphosphoinositol pentakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052723">
        <property type="term" value="F:inositol hexakisphosphate 1-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052724">
        <property type="term" value="F:inositol hexakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000830">
        <property type="term" value="F:inositol hexakisphosphate 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000831">
        <property type="term" value="F:inositol hexakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052846">
        <property type="term" value="F:inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052843">
        <property type="term" value="F:inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052845">
        <property type="term" value="F:inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071545">
        <property type="term" value="P:inositol phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051516">
        <property type="term" value="P:regulation of bipolar cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110162">
        <property type="term" value="P:regulation of mitotic spindle elongation (spindle phase three)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000036">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11950:FF:000002">
        <property type="entry name" value="Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037446">
        <property type="entry name" value="His_Pase_VIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040557">
        <property type="entry name" value="VIP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750">
        <property type="entry name" value="DIPHOSPHOINOSITOL PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12750:SF9">
        <property type="entry name" value="INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL-PENTAKISPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18086">
        <property type="entry name" value="PPIP5K2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="VIP1 N-terminal" evidence="17">
        <location>
            <begin position="85"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp fold RimK-type" evidence="16">
        <location>
            <begin position="302"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="836"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="839"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1054" mass="118557" checksum="BC54EE634B310D07" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAV6</accession>
    <name>A0A1A0HAV6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosomal protein L13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_11606</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711528.1">
        <property type="nucleotide sequence ID" value="XM_018854031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAV6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAV6"/>
    <dbReference type="GeneID" id="30027007"/>
    <dbReference type="OrthoDB" id="1882297at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="200" mass="22369" checksum="44A17CB69A672D68" modified="2016-10-05" version="1">MSDFQPVVVIDGKGHLLGRLASIVAKQLLNGQKVVIVRCEALNISGEFFRNKLKFHDYLRKATRYNRTKGPFHFKAPSRVFYKAIRGMIPHKTARGKAALERLKVFEGMPSPYDKKKRVVVPQALRVLRLKPGRKYTTVGKLSAAVGWKYESIVDQLEEKRKVRSAEYYAKKAAAAKKIAAATASTAESDAAKKLAAFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAW0</accession>
    <name>A0A1A0HAW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">type II protein arginine methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31819</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711769.1">
        <property type="nucleotide sequence ID" value="XM_018855928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAW0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAW0"/>
    <dbReference type="GeneID" id="30028904"/>
    <dbReference type="OrthoDB" id="17415at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF5">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="615" mass="70455" checksum="F7A3F8539DA55904" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAY1</accession>
    <name>A0A1A0HAY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31663</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711553.1">
        <property type="nucleotide sequence ID" value="XM_018855894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAY1"/>
    <dbReference type="GeneID" id="30028870"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="154" mass="17851" checksum="77CAF0BE8576EAAF" modified="2016-10-05" version="1">METTRGFLEMQEAEAGNTRGWKCKRLETQETGNARDWKRKRLEMQEAGIQETGIQETGIQETGDARGWYHKRLALQEAGNTRDWKNKRLETQEAGNTKVCSHKRRDNRVQNISKVRKGSRVREISKLHHENACVRLQVLSSRHSYIARFTTALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB33</accession>
    <name>A0A1A0HB33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine synthase 1</fullName>
            <ecNumber evidence="5">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">O-acetylserine (thiol)-lyase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">O-acetylserine sulfhydrylase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">O-succinylserine sulfhydrylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_31794</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of O-succinyl-L-serine into cysteine, the last step in the cysteine biosynthesis pathway. Can also use O-acetyl-L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-succinyl-L-serine + hydrogen sulfide = L-cysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:53816"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:136856"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711731.1">
        <property type="nucleotide sequence ID" value="XM_018855920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB33"/>
    <dbReference type="STRING" id="869754.A0A1A0HB33"/>
    <dbReference type="GeneID" id="30028896"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141223">
        <property type="term" value="F:cysteine synthase activity, acting on O-succinyl-L-serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000011">
        <property type="entry name" value="Cysteine synthase (o-acetylserine)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007989">
        <property type="entry name" value="PRK10717.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="19">
        <location>
            <begin position="43"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA21221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="375" mass="40867" checksum="8F21635289095DD1" modified="2016-10-05" version="1">MMKAGIRSTLRQNIAVGVRSIKISAAESEFKPPFVPLVADGFSDAIGNTPLIRLPKISKSVGRNIYAKAEYMNPGGSIKDRAAKFILDDAEAKNLIQPGGTVVEGTAGNTGIGLAHVCRLRGYKCVIYMPNTQSQSKIETLRLLGAEVHPVPAVAFDNPQNYNHQAKRHADLLENAVWTNQFDNIANRQAHIESTGPEIWAQLNGKVDAWTCSTGTGGTFAGVTRYLKHMDSKVKCVLADPPGSVLYSFVKSGGKDIVRGGSSFTEGIGQGRVTKNLKPEIDLIDDAIKISDEESIIMVYRLLDEEGIYVGGTSALNVVAALKIAETLPENANIVTILCDSAHKYSARIFSKKWLHEKNLWHVLPEHLQKYATLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBC4</accession>
    <name>A0A1A0HBC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peroxisomal ATPase PEX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Peroxin-1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Peroxisome biosynthesis protein PAS1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_31771</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the PEX1-PEX6 AAA ATPase complex involved in peroxisome biosynthesis. The complex acts as a protein dislocase complex that mediates the ATP-dependent extraction of the PEX5 receptor from peroxisomal membranes, an essential step for PEX5 recycling. Specifically recognizes PEX5 monoubiquitinated at 'Cys-6', and pulls it out of the peroxisome lumen through the PEX2-PEX10-PEX12 retrotranslocation channel. Extraction by the PEX1-PEX6 AAA ATPase complex is accompanied by unfolding of the TPR repeats and release of bound cargo from PEX5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711698.1">
        <property type="nucleotide sequence ID" value="XM_018855913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBC4"/>
    <dbReference type="STRING" id="869754.A0A1A0HBC4"/>
    <dbReference type="GeneID" id="30028889"/>
    <dbReference type="OrthoDB" id="2187at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904949">
        <property type="term" value="C:ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19526">
        <property type="entry name" value="RecA-like_PEX1_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000149">
        <property type="entry name" value="Nuclear valosin-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015342">
        <property type="entry name" value="PEX1-N_C-lobe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF12">
        <property type="entry name" value="PEROXISOMAL ATPASE PEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09262">
        <property type="entry name" value="PEX-1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="471"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="746"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1040"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA21188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1062" mass="118361" checksum="A4E04F1A1C7976B5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBL0</accession>
    <name>A0A1A0HBL0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_179466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711783.1">
        <property type="nucleotide sequence ID" value="XM_018854746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBL0"/>
    <dbReference type="GeneID" id="30027722"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="73" mass="8200" checksum="0B0C00A3E878DA48" modified="2016-10-05" version="1">MLVPTNIVYAVAKIPHGTVTRAITGVCSFYCKGCVYLCGVLKLLLSARRFRRLRERSSYLPATLKQSRTPQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBL5</accession>
    <name>A0A1A0HBL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_41099</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711788.1">
        <property type="nucleotide sequence ID" value="XM_018857355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBL5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBL5"/>
    <dbReference type="GeneID" id="30030331"/>
    <dbReference type="OrthoDB" id="10251154at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000378">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="271"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="10">
        <location>
            <begin position="352"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="492"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="493"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="535" mass="59744" checksum="24A668334B7F22B9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBN4</accession>
    <name>A0A1A0HBN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AP-2 complex subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_11916</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <location evidence="5">Coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711905.1">
        <property type="nucleotide sequence ID" value="XM_018854127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBN4"/>
    <dbReference type="STRING" id="869754.A0A1A0HBN4"/>
    <dbReference type="GeneID" id="30027103"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017104">
        <property type="entry name" value="AP2_complex_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009028">
        <property type="entry name" value="Coatomer/calthrin_app_sub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037091">
        <property type="entry name" value="AP2_complex_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55711">
        <property type="entry name" value="Subdomain of clathrin and coatomer appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="25"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="50"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037091-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="974" mass="106444" checksum="09335DEC08AC0C01" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBR4</accession>
    <name>A0A1A0HBR4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711827.1">
        <property type="nucleotide sequence ID" value="XM_018855933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBR4"/>
    <dbReference type="GeneID" id="30028909"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="413" mass="45922" checksum="1F67CCE72C4FC05A" modified="2016-10-05" version="1">MAPIFDSSDSSASETKFKRKRRGGKSWSRKSKRARNIELVAPGTPSGEAETTNGVDIIKRRSSHIIARLRVAWAHNKKSLHEALNIRPDLESESGNKTKRGPDEVDGQVKRPILARLHLFISEIADLYADEVPLKEGIDRCDPFKYQFGFGAEHYLTEMLRARKINPGCGSLAKSNGTLLAESPRSATNMETLEKPGKDNSIKYRCRGLFPVSTESVVSERTSTEISQPKNLPIFGSPVSEEESLASSESEIAFNAYEANLSVEKSGQPSTVSFDWDLTRSHDESEVEKLLDHLQLSVAASLQSAVDHSNDKKQKSRSASSESPHYLNSTRTESEWSVSNKNEHISDLLSLDSEHGYFSEMCCDTGDRDSTSGSVIIEDQHTPMNSGRGINEKPGRLTEVQILHNWLKARKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBU3</accession>
    <name>A0A1A0HBU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fructose-1,6-bisphosphatase</fullName>
            <ecNumber evidence="5">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-fructose-1,6-bisphosphate 1-phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_11986</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the FBPase class 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711991.1">
        <property type="nucleotide sequence ID" value="XM_018854136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBU3"/>
    <dbReference type="GeneID" id="30027112"/>
    <dbReference type="OrthoDB" id="10256725at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072593">
        <property type="term" value="P:reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005986">
        <property type="term" value="P:sucrose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000002">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000001">
        <property type="entry name" value="Fructose-1,6-bisphosphatase class 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020548">
        <property type="entry name" value="Fructose_bisphosphatase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006778">
        <property type="entry name" value="PRK09293.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556:SF1">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00124">
        <property type="entry name" value="FBPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class I N-terminal" evidence="14">
        <location>
            <begin position="16"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Fructose-1-6-bisphosphatase class 1 C-terminal" evidence="15">
        <location>
            <begin position="207"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="339" mass="37427" checksum="1843AD685165FD28" modified="2016-10-05" version="1">MSQSVSEQMKVETDIITLTRFILEEQQKYAQGATGELSLLLNGLQFAFKFIAHNIRRAELVKLIGISGTANSTGDVQKKLDVIGDEIFINAMKSSGNVKVLVSEEQEDLIVFEGGGRYAVCTDPIDGSSNIDAGVSVGTIFGVYRLKDDLKGLIKDVLRKGTEMVAAGYTMYGASAHLMLTTGHGVNGFTLDTQLGEFILTQPNLRIPKSRAIYSVNEGNSYYWPENVKKYIEDLKKPQDELKGKPYSARYIGSMVADIHRTLLYGGIFAYPADSKLKNGKLRVLYECFPMALLMEEAGGSAVNDKGERILDILPKEIHERSGIWLGSKGEVDRFLSYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCK1</accession>
    <name>A0A1A0HCK1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32179</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat PWP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712221.1">
        <property type="nucleotide sequence ID" value="XM_018855997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HCK1"/>
    <dbReference type="GeneID" id="30028973"/>
    <dbReference type="OrthoDB" id="3142434at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000219">
        <property type="entry name" value="Periodic tryptophan protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000602">
        <property type="entry name" value="Periodic tryptophan protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="142"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="186"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="348"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="390"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="476"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="9">
        <location>
            <begin position="753"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="864"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="887"/>
            <end position="907"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="934" mass="104964" checksum="8E5993C8444B803F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCQ7</accession>
    <name>A0A1A0HCQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712375.1">
        <property type="nucleotide sequence ID" value="XM_018855731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCQ7"/>
    <dbReference type="GeneID" id="30028707"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291744" evidence="1">
        <location>
            <begin position="17"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="56" mass="6055" checksum="D53E28DFC9C6D6F8" modified="2016-10-05" version="1">MRAGVALFLCWVVVLGWSFVGSVCGPGVSGSDVMIWKRGQKIGMCSDGHKSKVLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCR3</accession>
    <name>A0A1A0HCR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_39040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712271.1">
        <property type="nucleotide sequence ID" value="XM_018857085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCR3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCR3"/>
    <dbReference type="GeneID" id="30030061"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000247">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000106">
        <property type="entry name" value="MFS transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF29">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="541" mass="62149" checksum="C01A4E0C162C4D98" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDB7</accession>
    <name>A0A1A0HDB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_10969</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712570.1">
        <property type="nucleotide sequence ID" value="XM_018853891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDB7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDB7"/>
    <dbReference type="GeneID" id="30026867"/>
    <dbReference type="OrthoDB" id="2188412at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="574"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="602"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="681"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="732"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="162"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="240"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="445"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="244"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="277"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="287"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="498"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="765" mass="84990" checksum="27C9E98C3884905A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDE1</accession>
    <name>A0A1A0HDE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_146331</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712595.1">
        <property type="nucleotide sequence ID" value="XM_018854522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDE1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDE1"/>
    <dbReference type="GeneID" id="30027498"/>
    <dbReference type="OrthoDB" id="4095993at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="343" mass="36633" checksum="12D567DFA9A24F42" modified="2016-10-05" version="1">MSETGQLQNQPTKHETGAIGATLSPASFPCHFCGRAFSRKGTYGRHLDSKKGDAQHPAGQVEALRSGVVRRAARRAGEPVADEKAGRAPKKVKRQKSQIAKVYNSKTAVKEKNRARRRARDLRLKAQLQAHEWYIGMLSGPQGLAQGPAGRAENASLEAGKQAHRSQGLESGPERSQKSGQKSDAEIYGARYAYAVASNVPPSQWPQKNGYPGDAEFGATVGQLTALAGMAPYNRSAPGPQYFQGLGPQSGGGAAAQDSTGAAALEQVFAWHAHWAQLGPSTKRALWQAECDRALRTHLHGASLCQISGAAAVVREKTRQLCEQYSQGAFLDSILTDSGPETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDH9</accession>
    <name>A0A1A0HDH9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_39859</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712635.1">
        <property type="nucleotide sequence ID" value="XM_018857191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDH9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDH9"/>
    <dbReference type="GeneID" id="30030167"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01370">
        <property type="entry name" value="KISc_KIP3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF13">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF19 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="9">
        <location>
            <begin position="14"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="776"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="780"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="149"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="855" mass="92877" checksum="59405D41585ED902" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDT2</accession>
    <name>A0A1A0HDT2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712579.1">
        <property type="nucleotide sequence ID" value="XM_018855759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDT2"/>
    <dbReference type="GeneID" id="30028735"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="71" mass="8285" checksum="5D152B4D67BC8422" modified="2016-10-05" version="1">MLKTLFMLVLNLSVHCRRNKTKIIKYMHTWFLSSRSFMILGSYQTAALHVFSNTTLNSATIPKALIIRQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE03</accession>
    <name>A0A1A0HE03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_31174</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712737.1">
        <property type="nucleotide sequence ID" value="XM_018855791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE03"/>
    <dbReference type="STRING" id="869754.A0A1A0HE03"/>
    <dbReference type="GeneID" id="30028767"/>
    <dbReference type="OrthoDB" id="7813104at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000001">
        <property type="entry name" value="mitochondrial import inner membrane translocase subunit TIM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="28"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="98" mass="10924" checksum="784203A4290A9B7B" modified="2016-10-05" version="1">MALFGSSTTSAAPAVTQLSNQIKTQLQEQIAQELAVANATELVNKITQNCFDKCIDQPQDLISPQQNTCVSQCLEKYMRSWNVISKTYISRIQNEKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEQ0</accession>
    <name>A0A1A0HEQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PLP-dependent transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77841</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712946.1">
        <property type="nucleotide sequence ID" value="XM_018858779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEQ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEQ0"/>
    <dbReference type="GeneID" id="30031755"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43510:SF1">
        <property type="entry name" value="AMINOTRANSFERASE FUNCTION, HYPOTHETICAL (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="3">
        <location>
            <begin position="50"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="381" mass="42571" checksum="B7E2C3EB437B31E2" modified="2016-10-05" version="1">MVKQEAFAVEQFMDKHETSIVYNMGETCVDSLTIPEIVGMDKEASAKIAQDLLGTKLTYGHIKGSPELRGAIAALYEGSGVTAEDVVVTNGAIAANFLTFYTVVNPQEHVIVVAPSYQQLSSVPEMFGAKVDMFNIRAEDLYRPRLDELRTMISQNNTRLLVINNPNNPSGFVWENDILAEIVALCKEHDVTIMCDEVYRPLFHFQTQEPVKSIVDFGYDKAISTGSMSKAFSLAGLRVGWIVTKNKEYSAGFWEKRDYNTISVLMLDDTVAAVALQNTERILERNHKICQTNLAVLQKFVDEHSDKVSWVRPRGGSTCFLQLHIKATSMEFAEKLIEKHGTLVVPGEVFGMPGWLRLGFGNSTHDISEGLRCFGELLQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HER9</accession>
    <name>A0A1A0HER9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">OPT superfamily oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_38733</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712992.1">
        <property type="nucleotide sequence ID" value="XM_018857040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HER9"/>
    <dbReference type="STRING" id="869754.A0A1A0HER9"/>
    <dbReference type="GeneID" id="30030016"/>
    <dbReference type="OrthoDB" id="627262at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645:SF0">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER YGL114W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="529"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="584"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="623"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="662"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="693" mass="74818" checksum="EDD96D5E27D7A2D1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF16</accession>
    <name>A0A1A0HF16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vanadate resistance protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_39416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ANP1/MMN9/VAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713092.1">
        <property type="nucleotide sequence ID" value="XM_018857136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF16"/>
    <dbReference type="STRING" id="869754.A0A1A0HF16"/>
    <dbReference type="GeneID" id="30030112"/>
    <dbReference type="OrthoDB" id="204164at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000163">
        <property type="entry name" value="Van1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052086">
        <property type="entry name" value="Mannan_Polymerase_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083:SF5">
        <property type="entry name" value="MANNAN POLYMERASE I COMPLEX VAN1 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43083">
        <property type="entry name" value="MANNAN POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03452">
        <property type="entry name" value="Anp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508829" description="Vanadate resistance protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="446" mass="50773" checksum="01E77C817DB130E2" modified="2016-10-05" version="1">MHRAAWVLCVCLLFLWFLGGRLNRQNGQLGQAPAPESAPDAGDTHRLYTVTKDGVTEEDTGEAAEEYSDKMFKETVEFFDLDNSLGARRKGPDGEDTDVVLFLMPLRNAEHVLPMAFHNLMNLTYDHSLIDIAFLVSDCSPGDTTLQTVFDFSVALQNGTLADDMLSAEQAKKDSNVKGTNELYQLYMDQDYMDNVHRAYNSPESYHKGYRTPFRSVSIYVKDFGQVVGQGFSDRHAVKVQGIRRKLMGRARNWLTASALQADHSWVYWRDVDIETCPGLVIQDLMKFDYDVMVPNIWRPLPTYLGGEQPYDLNSWIESEPALELAKKLDEDDVIVEGYAEYPTYRVHLAYIRDSEGNPDEVVDLDGVGGVSILAKAKIFRQGVNFPAFTLWNHAETEAFGKLARRMGFKVGGLPHYTIWHIYEPSEDDLKHIAQLERKKRREKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFC8</accession>
    <name>A0A1A0HFC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Trimeric LpxA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_39178</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713098.1">
        <property type="nucleotide sequence ID" value="XM_018857106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFC8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFC8"/>
    <dbReference type="GeneID" id="30030082"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03357">
        <property type="entry name" value="LbH_MAT_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000025">
        <property type="entry name" value="Hexapeptide-repeat containing-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF54">
        <property type="entry name" value="ACETYLTRANSFERASE, CYSE_LACA_LPXA_NODL FAMILY (AFU_ORTHOLOGUE AFUA_2G08430)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Maltose/galactoside acetyltransferase" evidence="4">
        <location>
            <begin position="30"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="230" mass="25461" checksum="AC6F291F5BBC86F7" modified="2016-10-05" version="1">MEAPLKTTLDDKLVKFAHENLNHVPQSHEYDKMISSVPYNCFDKELWMRRNLAHEMALDYGNIRMADHEFSFEKHQAARVEHLGKIFGNIQPDFFIEPPFFVDYGFNVKIGKRFYGNFNITFLDCTLIVIGDNVMVGPNCCFTTATHPTDPTQRLAGVEYAKPIYIGNNVWVGAGVNVMPGVTIGDGAVIGAGAVVTRDVPANTVVVGVPAKVVKHMTPSEAKQDMGGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFJ7</accession>
    <name>A0A1A0HFJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aldo/keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_94313</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713405.1">
        <property type="nucleotide sequence ID" value="XM_018859387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFJ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFJ7"/>
    <dbReference type="GeneID" id="30032362"/>
    <dbReference type="OrthoDB" id="37537at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19077">
        <property type="entry name" value="AKR_AKR8A1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050791">
        <property type="entry name" value="Aldo-Keto_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625">
        <property type="entry name" value="AFLATOXIN B1 ALDEHYDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43625:SF78">
        <property type="entry name" value="PYRIDOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="2">
        <location>
            <begin position="11"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="38167" checksum="4EFD50C2F0BA1CDC" modified="2016-10-05" version="1">MTPVNIPGKFGYGSMSLTWTPTPKTEAEAIQAIKYVLDKHQVRFVNGGEFYGPDSVNLKYLGAFWKHFGCDYPDLVISVKGSIDVTTLKPDGSRESISRSINNFVSYFPQNKLQRPKLIFEIARVDTSVPYEQTIGFINEFVGDGVIDGISLSEVGVGSIEKALSVAPISSVEVEFSLLCQDIFSNGVLKKAAEENLTIVAYSPLCRGFLTDRSADDFSAFFDLCHQPGDIRGHIDRFSKENFFENLKIVAKLKDYAETKGTTLESLAISWIVAYSELENYNGISKFPKIIPIPSGSSSDKIDQNLGQIVSLSKADLDDIKKITDCFEVKGYRYNAKVKHLDFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM4</accession>
    <name>A0A1A0HFM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA damage checkpoint protein LCD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_85317</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713435.1">
        <property type="nucleotide sequence ID" value="XM_018859247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFM4"/>
    <dbReference type="GeneID" id="30032222"/>
    <dbReference type="OrthoDB" id="4078000at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018622">
        <property type="entry name" value="DNA_damage_chkpnt_Lcd1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09798">
        <property type="entry name" value="LCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="110"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="40"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="691" mass="77495" checksum="0B318338DC91D359" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ4</accession>
    <name>A0A1A0HFQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_34396</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713208.1">
        <property type="nucleotide sequence ID" value="XM_018856468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFQ4"/>
    <dbReference type="GeneID" id="30029444"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20024">
        <property type="entry name" value="FH_FOXJ2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FHA" evidence="8">
        <location>
            <begin position="71"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="9">
        <location>
            <begin position="232"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="232"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="348"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="533"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="551"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="65613" checksum="0F744AA5520721CE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFW1</accession>
    <name>A0A1A0HFW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitobiosyldiphosphodolichol beta-mannosyltransferase</fullName>
            <ecNumber evidence="3">2.4.1.142</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Asparagine-linked glycosylation protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_75927</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.142" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713224.1">
        <property type="nucleotide sequence ID" value="XM_018858449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFW1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFW1"/>
    <dbReference type="GeneID" id="30031425"/>
    <dbReference type="OrthoDB" id="614844at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004578">
        <property type="term" value="F:chitobiosyldiphosphodolichol beta-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026051">
        <property type="entry name" value="ALG1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036">
        <property type="entry name" value="BETA1,4 MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13036:SF0">
        <property type="entry name" value="CHITOBIOSYLDIPHOSPHODOLICHOL BETA-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13692">
        <property type="entry name" value="Glyco_trans_1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="51520" checksum="130AFC6BD5342625" modified="2016-10-05" version="1">MNYAYFGELVLSRNWILWLLFAYFILPFTVYLFVPFFIHRKSVLNKKSVSIFVLGDIGHSPRMCYHARSFASLGFAVNLCGYLESEPPQGIIEDVNIDIFPIQAIQNRRNLPYLLFAITKAVLQIFSLFELCYLLSGSQYYMVQNPPSMPLVAVLVIFIKLFSPRSKLIIDWHNLNYSILNLRFQNVRHPLVQLMKSYEKFFSRFSWLNITVTEQMKHYLATDFGVKSDRILAFHDRPGPQFSPLSQEKIKQVFAEHELFTSVPEIEQYKVLVTATSFTPDEDLGILLECLKIYHDSSSDATPPIFVLVTGKGPLKSQFLKCVEGLGFLSRVIVKSAWLSAEEYPLLLGVADLAVSLHTSLSGIDLPMKIVDFFGVGVPVVTLSFPAIGELVKDGVNGLVVSENITKSVPGDLYKLVALILGDDSRLAKLKLGAMSESKQRWNENWMQVLAPVVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG45</accession>
    <name>A0A1A0HG45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinesin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_29446</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713348.1">
        <property type="nucleotide sequence ID" value="XM_018855403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG45"/>
    <dbReference type="STRING" id="869754.A0A1A0HG45"/>
    <dbReference type="GeneID" id="30028379"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="8">
        <location>
            <begin position="4"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="494" mass="56151" checksum="DE2A11C14F072D7B" modified="2016-10-05" version="1">MSTSIKTIVRVRPLLPREDASVCLVHMPSDEPGKTYLDHSKGSKVYTFDECVWSHSKNEENYESNASFYQKCGYHLLEHLYQGYNVCLLAYGQTGSGKTYTMMGQKNDYGIIPLMVRDILRQRDALVMDKINCEMTFSFIEIYNEKVKDLLGGSKVCRVREDPVLGPYIEGLTNVSVESFEEFFEFLKTGNSLRMTASTKMNEASSRSHAVLTFTLKQTRFAENSDSSQSVGAPAEEMVSNIKLVDLAGSERLAKTQVFHQSDRLKEGAQINKSLTVLGRCINLLSLGEKGVVPYRDSTLTFLMKENLSGNSKTSMIFCVSPTDYDETHQTLNYATQVKKIKTKAKASASKLLAPPVDWAALHVEDQSIVERLREEISALKKQIHESQIAEKDEGADHLITYFTNESQRQSFEIKYLRNRLQSKNEEFEEIQGHNRYLKSEIKLLLRKQSSIEQKRLVARASETIKRCETEAQMIGEIVEEFVSCPKRAREKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGH3</accession>
    <name>A0A1A0HGH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribosomal RNA small subunit methyltransferase NEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_29512</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713434.1">
        <property type="nucleotide sequence ID" value="XM_018855422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGH3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGH3"/>
    <dbReference type="GeneID" id="30028398"/>
    <dbReference type="OrthoDB" id="269804at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000003">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="253" mass="27906" checksum="0A82FB36873F2960" modified="2016-10-05" version="1">MTDAKQDSRVQDKPAAGAPPPASFVPSRPQVLTSKDKDTKRMVVVLSQACLETHKISTGAAGGDKYALLNCDDHQGLLRKMGRDIAEARPDITHQCLLTLLDSPINKAGKLQVFIHTARGVLIEVNPSVRIPRTFKRFSGLMVQLLHKLSIRSVDSEEKLLKVVKNPITDHLPTKCRKVTLSFDAKIRRVQDYVQTLADDESICVFVGAMARGQDTFADEYVDEKIGLSDYPLSASVACSKFCHGCEDVWGIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGK7</accession>
    <name>A0A1A0HGK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713617.1">
        <property type="nucleotide sequence ID" value="XM_018855455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGK7"/>
    <dbReference type="GeneID" id="30028431"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="80" mass="8548" checksum="D6617CEDC1CF0017" modified="2016-10-05" version="1">MAERALGLLLYSVLGPNTKSCFVEKSGAMALAAAKQCLFRHRTGVVVPYQCGQDGCKQQSELFCAVMDRSVGCENCGAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGK8</accession>
    <name>A0A1A0HGK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713785.1">
        <property type="nucleotide sequence ID" value="XM_018855485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGK8"/>
    <dbReference type="GeneID" id="30028461"/>
    <dbReference type="OrthoDB" id="4096810at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="452" mass="49911" checksum="50B1FD3D743164D6" modified="2016-10-05" version="1">MAAEEGENVKFLRGTSNTAAKPQGTASSISRRANSYQLQSNAGEPRTSEDLRQTALRNGLTRVNSASRVDGFTNSGRSHMTPKNRNIPMVAAPVVGRISDNRWGTYSLNQPINNVLVPTNESISEPHEDLNVAQKASISPLTQIHRPTGKTHSFDQAGIEINESKRGDLRSEKTSRGSSRSSLRDFHPRERSVGRFPDKKSNHNSMFSLVPMGLSDSEAEVCGDERALLIEADKSEDLEALYEENYEEAYLQIPGLADATSVLDFMKGHEKAQETKQSIDAVSIGSSLDRPTTRTHQKLLDMKLLRLRQEAASAASTATLAMGGLRNLPEYAVKIQHESVVSQWTQIRLRFSSHPENTEGRRTNIACRAGVLGSIKRNEAELAMKAGDRRALESRITKDNVASYLHEMWHDQSFETQPGDVFFDAGNLVDNGDQSASADMMQMAKNVLLTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGP8</accession>
    <name>A0A1A0HGP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_22877</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713509.1">
        <property type="nucleotide sequence ID" value="XM_018855215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGP8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGP8"/>
    <dbReference type="GeneID" id="30028191"/>
    <dbReference type="OrthoDB" id="427030at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001907">
        <property type="entry name" value="AZF1 (YOR113W)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000130">
        <property type="entry name" value="Spalt-like transcription factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002157">
        <property type="entry name" value="Transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="84"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="242" mass="27044" checksum="D79DA773BB3B2EAF" modified="2016-10-05" version="1" fragment="single">RPLLGATKVDQLMLVIQAREKGNTTAIKQSTDGSILASPNSDHPKDSVLPLAVGLVGGVDKPLKTENANDGEPSKKRKKRGKTQECPYCFKQFHQSTHLDVHVRSHIGLKPFECTFCGKRFTQGGNLRTHMRLHTGEKPFVCNICNHNFSRKGNLAAHMLTHNKEKPFECKLDNCDKSFTQLGNLKSHQNKFHLPTLTKLTQTFASITGDALALLPEEEKQLLEYFAKLYKNANKGIRGRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGT3</accession>
    <name>A0A1A0HGT3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Longevity assurance proteins LAG1/LAC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_37884</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713687.1">
        <property type="nucleotide sequence ID" value="XM_018856926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGT3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGT3"/>
    <dbReference type="GeneID" id="30029902"/>
    <dbReference type="OrthoDB" id="3053196at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF11">
        <property type="entry name" value="CERAMIDE SYNTHASE LAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="12">
        <location>
            <begin position="162"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="403" mass="46846" checksum="0689B84DBB66275C" modified="2016-10-05" version="1">MRQTSDSTISQAARKGVSGGNNNVGESSATDYSTANSLQRRQQRDGAKLAEPESRLESGSSNSILLTYREISQHHAWFFPCLIILAHFAVFFMTDEKSDVHQMLAKMMTVSYHIEGTDQYGKGEYDFYFVGFYSLCFTFLREFMMVCIFRPAALYFGIRKETKIKRFMEQTYSMFYYGMLGPFGLWVMSRTPLWLFETTPFYLEYPHTTHDIYFKVYYLGQAAFWVQQSVVLLLQLEKPRKDFHELIAHHIVTIALIWNSYAFHFTWMGLAVYVTMDVSDFFLSFSKTLNYLGSPLAGPFFVIFVGVWIYLRHWINLKIIWSVMTEFLTVGECTLNNATQLYKFWFSQPIVLGLLVALQLLNLYWLFLICRILYRYAVGGVAADERSDEESDGETTVDAKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGV5</accession>
    <name>A0A1A0HGV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CDC45-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_63598</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC45 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713706.1">
        <property type="nucleotide sequence ID" value="XM_018858334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGV5"/>
    <dbReference type="GeneID" id="30031310"/>
    <dbReference type="OrthoDB" id="10258882at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003874">
        <property type="entry name" value="CDC45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507">
        <property type="entry name" value="CDC45-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10507:SF0">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 45 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02724">
        <property type="entry name" value="CDC45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="178"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="692" mass="77480" checksum="3A3C5353006B1FB7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH50</accession>
    <name>A0A1A0HH50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription activator of gluconeogenesis ERT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_34478</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription factor which regulates nonfermentable carbon utilization. Activator of gluconeogenetic genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713807.1">
        <property type="nucleotide sequence ID" value="XM_018856477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH50"/>
    <dbReference type="STRING" id="869754.A0A1A0HH50"/>
    <dbReference type="GeneID" id="30029453"/>
    <dbReference type="OrthoDB" id="2538135at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045991">
        <property type="term" value="P:carbon catabolite activation of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045013">
        <property type="term" value="P:carbon catabolite repression of transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF1">
        <property type="entry name" value="TRANSCRIPTION ACTIVATOR OF GLUCONEOGENESIS ERT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="14">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="15">
        <location>
            <begin position="408"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA23326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="528" mass="58969" checksum="585FCC75CD76324A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHA6</accession>
    <name>A0A1A0HHA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714045.1">
        <property type="nucleotide sequence ID" value="XM_018859465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHA6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHA6"/>
    <dbReference type="GeneID" id="30032440"/>
    <dbReference type="OrthoDB" id="4089169at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="724" mass="79773" checksum="236D488A04852F8E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHA7</accession>
    <name>A0A1A0HHA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713704.1">
        <property type="nucleotide sequence ID" value="XM_018855472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHA7"/>
    <dbReference type="GeneID" id="30028448"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291802" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13641" checksum="537BDCFFF0815DF4" modified="2016-10-05" version="1" fragment="single">MQDWGTAMAVLLLSGVSRVTPDCCPVVQAATRHVCRRSVKEPSLAGFLFLRLQGEVPCDCAIFRRARKPEHRTCRNLGHAEMAEGEEKKRNKLFEYQIFESGLDLLLAGGQLTGHAWNRCAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHE0</accession>
    <name>A0A1A0HHE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_35020</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713773.1">
        <property type="nucleotide sequence ID" value="XM_018856556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHE0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHE0"/>
    <dbReference type="GeneID" id="30029532"/>
    <dbReference type="OrthoDB" id="4457at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070156">
        <property type="term" value="P:mitochondrial phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.380:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000053">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00469">
        <property type="entry name" value="pheS_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="153"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="16">
        <location>
            <begin position="364"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00073229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA23292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="457" mass="52841" checksum="7BE772B6664B6803" modified="2016-10-05" version="1">MISLTRLSGNPGARSIAIGVCAHKFARLYANYPPLITVDGKAYETDEWTNVPKLILALTDRQLHQKPNHPIGILRELIQNNFKGLGYTFYNDFRPTVTTYQNFDVLEFPADHPGRSKLDTYYLNKGHLLRTHTSAHEHECFQICETPGYFITADVYRRDEIDRTHYPAFHQMEGARLWSKQNPELLKELQNDIDSIPNTNIIVEDPFRENPSNAENPKQQYMTDPEVRLVATHLKKTVEYMVNLVFEKARESALKAGSKEEYLNEPLKVRWVEAYFPWTSPSWEIEVWWKGEWLECCGCGVVQQQILLNSGLPVDKLGWAFGVGLDRIAMLLFGIPDIRLFWSLDERFARQFTQGTISTFQPYSKYPGVKRDVSYWLPSESPLHTNDVMEIVRLHGGDLVESVVLKDEFVHPKTGRKSQCYGIHFQSMDRNLTNSEVNEIHKLVEKDLIETYGVEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHF9</accession>
    <name>A0A1A0HHF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin fusion degradation protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_9705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713922.1">
        <property type="nucleotide sequence ID" value="XM_018859444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHF9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHF9"/>
    <dbReference type="GeneID" id="30032419"/>
    <dbReference type="OrthoDB" id="422728at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030894">
        <property type="term" value="C:replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006274">
        <property type="term" value="P:DNA replication termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900182">
        <property type="term" value="P:positive regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072665">
        <property type="term" value="P:protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.50:FF:000001">
        <property type="entry name" value="Ubiquitin fusion degradation protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="5">
        <location>
            <begin position="22"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="6">
        <location>
            <begin position="122"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="310"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="356" mass="39343" checksum="406191196E1801C4" modified="2016-10-05" version="1">MFSGFGSNFFGGASFAPINSKFEDYFRCYPVAMMPDNVRKDDSNFGGKIFLPSSALNKLTMLHIRYPMLFELTNEAENLTSHSGVLEFVAEEGRAYLPQWTMETLKLQPGSLIKIASCDLPNGQFVKLEPQSVEFLDISDPKAVLENALRKFSTLTVNDVIEIDYNDTRFGIKVLEVKPESTNKGICVVETDLETDFAPPVGYVEPEYKPKGAKSSASPIDPKKVNRSAGAATMAHSIGYANIVHEGNNQKVYKGTGQKLSGKEVGSHDTKRITIDDLDPNATPVELNLPENQIFLGWPVVLPVLEKKEESAPSVSYQGEGKSLRQSKKRTTVTSNSGHDLLKNHSRSPDYIEIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHL3</accession>
    <name>A0A1A0HHL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7082 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_19569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713976.1">
        <property type="nucleotide sequence ID" value="XM_018854869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHL3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHL3"/>
    <dbReference type="GeneID" id="30027845"/>
    <dbReference type="OrthoDB" id="1751210at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055509">
        <property type="entry name" value="DUF7082"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39463">
        <property type="entry name" value="MEDUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39463:SF1">
        <property type="entry name" value="MEDUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23305">
        <property type="entry name" value="DUF7082"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7082" evidence="1">
        <location>
            <begin position="23"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="183" mass="20967" checksum="FABB536701415A44" modified="2016-10-05" version="1" fragment="single">LIRTSRLSPDYIRENLDKINVPIKLKTDRSFGQMETDWTEEEVSNERRLVSLHISRESVTTFNICFSPVAPGAVAKDNTDLIISCIRWEEKDVMVVTSVDIILVLEALVGESFSVDEKSRIRRNLQFLKPSNIARATCGRLFSSLMAMDNPRPRNIEKDVKVFRWSNFFDALNKVLSKFSANP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI25</accession>
    <name>A0A1A0HI25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit epsilon</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_36654</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPI-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the COPE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714295.1">
        <property type="nucleotide sequence ID" value="XM_018856766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI25"/>
    <dbReference type="STRING" id="869754.A0A1A0HI25"/>
    <dbReference type="GeneID" id="30029742"/>
    <dbReference type="OrthoDB" id="310217at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006822">
        <property type="entry name" value="Coatomer_esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10805:SF0">
        <property type="entry name" value="COATOMER SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04733">
        <property type="entry name" value="Coatomer_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016478">
        <property type="entry name" value="Coatomer_esu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="313" mass="34839" checksum="D522F5D6841F9BE3" modified="2016-10-05" version="1">MSAPPDSGELFTIRNQFYTGQHTKVAAYDWALFSPQAQLKVYEFQVRSALALAHDPAALLGKGRAAFPEHPALLAVLQAWSDVSASGVDDASYFAAVGDAAFEAQAVLAALYLVKYRQDVDGAISLLARFSARGTENALELEPHLLLVQLHLHKENFAEASRVYQRFQTLPFDARDDIIYHVMESWINSVKGQADNISNAYYFYDELLSSDFDDDVQGRFHNLSALFVMTLQLKHFPEAQEILDQVAALDYRGTGAANLVANRITYEYLTNNGANVVALLKELAAADPAHQLLTDFREKNERFDAIVEKYLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI56</accession>
    <name>A0A1A0HI56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_38369</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714048.1">
        <property type="nucleotide sequence ID" value="XM_018856993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI56"/>
    <dbReference type="STRING" id="869754.A0A1A0HI56"/>
    <dbReference type="GeneID" id="30029969"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034643">
        <property type="term" value="P:establishment of mitochondrion localization, microtubule-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097753">
        <property type="term" value="P:membrane bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090149">
        <property type="term" value="P:mitochondrial membrane fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140572">
        <property type="term" value="P:vacuole fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00302">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="6">
        <location>
            <begin position="24"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="5">
        <location>
            <begin position="729"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="616"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="816" mass="92636" checksum="90250019C9C22F88" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HID4</accession>
    <name>A0A1A0HID4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_30292</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714410.1">
        <property type="nucleotide sequence ID" value="XM_018855603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HID4"/>
    <dbReference type="STRING" id="869754.A0A1A0HID4"/>
    <dbReference type="GeneID" id="30028579"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17871">
        <property type="entry name" value="GPN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000338">
        <property type="entry name" value="GPN-loop GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030231">
        <property type="entry name" value="Gpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="320" mass="36373" checksum="CCF24B6487E60D17" modified="2016-10-05" version="1">MFGQVVIGPPGSGKSTYCHGMYQFLSAIGRKSTIINLDPANANLPYPSCSFDIRDFVSVEEIMESQNLGPNGGLMYALESLSENALDSIMKEWEEIGKNSYLLFDCPGQVELYTHHNAFFKIFKRLENKTDARLCVVSLVDSLYLTSPSLYVSVLLLSLRSMLQLDLPHINVISKIDKLQSYGKLAMRLDFYTEVQDLKYLGKFLQKESPSLLGKNYVRLSEMIAELIEDYNLVSFEVLAIDNKQTMINILQVIDKANGYLYGSTEIGGDLVWTQATRNGWSTSSDIIDIHERWVDNKQLYDKIECERDLRSVQEETLDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIG2</accession>
    <name>A0A1A0HIG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Pleiotropic drug resistance protein PDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_30064</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714109.1">
        <property type="nucleotide sequence ID" value="XM_018855546.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HIG2"/>
    <dbReference type="GeneID" id="30028522"/>
    <dbReference type="OrthoDB" id="245989at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03233">
        <property type="entry name" value="ABCG_PDR_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03232">
        <property type="entry name" value="ABCG_PDR_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000054">
        <property type="entry name" value="ABC multidrug transporter atrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029481">
        <property type="entry name" value="ABC_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034001">
        <property type="entry name" value="ABCG_PDR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034003">
        <property type="entry name" value="ABCG_PDR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005285">
        <property type="entry name" value="Drug-R_PDR/CDR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010929">
        <property type="entry name" value="PDR_CDR_ABC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00956">
        <property type="entry name" value="3a01205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19241">
        <property type="entry name" value="ATP-BINDING CASSETTE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14510">
        <property type="entry name" value="ABC_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06422">
        <property type="entry name" value="PDR_CDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="529"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="605"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="641"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="670"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1208"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1241"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1285"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1331"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1362"/>
            <end position="1389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1481"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="167"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="872"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1513" mass="169503" checksum="477284CDCB913DF5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH4</accession>
    <name>A0A1A0HIH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_36715</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714291.1">
        <property type="nucleotide sequence ID" value="XM_018856770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH4"/>
    <dbReference type="GeneID" id="30029746"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051063">
        <property type="term" value="F:CDP reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046704">
        <property type="term" value="P:CDP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006240">
        <property type="term" value="P:dCDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.70.20:FF:000001">
        <property type="entry name" value="Ribonucleoside-diphosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="14">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="781"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="799"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="826"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="869" mass="97338" checksum="62D23599D6C0C248" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIR2</accession>
    <name>A0A1A0HIR2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_111907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714209.1">
        <property type="nucleotide sequence ID" value="XM_018853927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIR2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIR2"/>
    <dbReference type="GeneID" id="30026903"/>
    <dbReference type="OrthoDB" id="4093693at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="371" mass="39195" checksum="7DF11FF38B6E8566" modified="2016-10-05" version="1">MSSRPKKNTKARAPPADKDAFRARREASFQLHDEADLVNFRADALSRYISNQRLLDNVALSLVHTAQIVPPPSFPSRTSGDRLYAESASDAELEAAVRGLKAADLYSGDLRLMRAKQRVLAQELAAAELRADGVLGPEARFQRDAVATLAAAQARCADADSLAALESALADTLEQARVQFGRRHELRANAVRKYAVAAHELAPGAVQQAPAGYDARRAMVLAAPGPMHDARYADGYMAPEYPEFGMLADGRGPGGADAALPFDPQLALAMLAGEAYLKESAGGAVAGHPDGVAPGHEAMGAAVDMDDIDQYLADPDAGDDMDDMHALMDFDGPGKPDDAMPFGLGPEPGAADLDDDPFGVDFLSLMGPGME</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIX8</accession>
    <name>A0A1A0HIX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_10016</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714274.1">
        <property type="nucleotide sequence ID" value="XM_018853671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIX8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIX8"/>
    <dbReference type="GeneID" id="30026647"/>
    <dbReference type="OrthoDB" id="10018191at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000502">
        <property type="entry name" value="Zinc finger protein 710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="153"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="157"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="352" mass="37844" checksum="5EA9B6C5731FA881" modified="2016-10-05" version="1">MSNKMEYRLPPINSLSLNFPLLIPQSYHSGAQHVHGLSSPQMAAHGFLRGSFLHANHVLPQAPPLAGPPRASAYGAPSWLPLTVPATHISGQPARPSPAGFPVGLAEYTLSQPSAARGAECSPPQVAAVGVPPMHVGSNSLPLKSLAQGAAPFLQQPSPSSTRRSSSISPLVRSADSPQEGAESDAAPSICASADASGQSDQARELKRRKKRQCPECRLFFSNLATHKSTHLNPTARPHVCNVCSRGFARPNDLSRHYKCHWKETGADNGQFKCPFKNGPAPGQCSHTLGIFSRCDTYKNHLKAIHFDYPGGTRKNERATSPGCCGLCQKQFLSVDEWLVTHVDTQLCESMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIZ5</accession>
    <name>A0A1A0HIZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FIST domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714466.1">
        <property type="nucleotide sequence ID" value="XM_018856750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIZ5"/>
    <dbReference type="GeneID" id="30029726"/>
    <dbReference type="OrthoDB" id="4080562at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="38879" checksum="04162787EB6FA8DE" modified="2016-10-05" version="1">MSGLRRIATAASDGAAFVARTVVAPPGDALLPVPAGLAARSALFLATPQNLAATIENAILLHQHMGLQVVVAGVDRTVPHAAASGVSQLWMDAPMKIGRSTLLADSDPPPAVRESDGIHPVGAKVPWRSAAGSLCLDVAGHRIDLELANTAFATSTLTTLFYFQKRDAAATSAGDHMGQTLSALHVTLPRLAPALRAPVAHDRWTPLTDSEHLVVSGCTGNLVKAINGQPAARFLEQNARLMREASKDTKVYVKVYGAGAGAAAPPARHEVIAGGGGWGAKAGLLALSPGAHVRTGDRVEFFMVTPADRFRRLAAAVAPRQFLFECAPEATQYADLAGASRTVEHLFGASSEAGFVADGLSHTSAGETLSFAFGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ55</accession>
    <name>A0A1A0HJ55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Micro-fibrillar-associated protein 1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36263</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714512.1">
        <property type="nucleotide sequence ID" value="XM_018856714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ55"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ55"/>
    <dbReference type="GeneID" id="30029690"/>
    <dbReference type="OrthoDB" id="1111734at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="2">
        <location>
            <begin position="77"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="199" mass="21640" checksum="F4E0CCCE6CE8D76D" modified="2016-10-05" version="1">MQRESDGGSGSGTGADSSADSSAGSGSDSDASSSGSDSALRLQRPVFVKKKLPPKAAGSRGPARAALAKAEHLQQVDSKTLEKVPFDGIDDRDDMRPEDEYTQWKLREAARKKRDALLLEETELSKEDAVRRKRGLVRTGPLPDLDENGGGTSFSKGPSIGSKLGAFYTEQLDEKLLKRDYKDIEGREDHSRPTKYKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD4</accession>
    <name>A0A1A0HJD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="19">ETF-QO</shortName>
            <ecNumber evidence="19">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_76905</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 19">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + oxidized [electron-transfer flavoprotein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="19">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ETF-QO/FixC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="19"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714478.1">
        <property type="nucleotide sequence ID" value="XM_018858605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJD4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJD4"/>
    <dbReference type="GeneID" id="30031581"/>
    <dbReference type="OrthoDB" id="437331at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000015">
        <property type="entry name" value="Electron transfer flavoprotein-ubiquinone oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049398">
        <property type="entry name" value="ETF-QO/FixC_UQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007859">
        <property type="entry name" value="ETF-QO/FixX_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617:SF107">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21162">
        <property type="entry name" value="ETFQO_UQ-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05187">
        <property type="entry name" value="Fer4_ETF_QO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9 19" id="KW-0274">FAD</keyword>
    <keyword evidence="6 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 19" id="KW-0408">Iron</keyword>
    <keyword evidence="14 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 19" id="KW-0813">Transport</keyword>
    <keyword evidence="15 19" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="20">
        <location>
            <begin position="582"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA23997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="622" mass="68349" checksum="5B12AC30524FD2B9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJE6</accession>
    <name>A0A1A0HJE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_118136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714602.1">
        <property type="nucleotide sequence ID" value="XM_018854099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJE6"/>
    <dbReference type="GeneID" id="30027075"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="119" mass="13557" checksum="83F4B764237DBF01" modified="2016-10-05" version="1">MLIIRLALKLGVAFKTFPPSPVPLILYTTPVSFLSSCSLLHFPLFSHLAFKFASLLFWRAETVSLTSCFLSLHSISLLSFCFSYSFNAFFQNFSAFLILTPSAWLIKLLHFSPYFLFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJH7</accession>
    <name>A0A1A0HJH7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Delta 8-(E)-sphingolipid desaturase</fullName>
            <ecNumber evidence="6">1.14.19.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_77136</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Delta(8)-fatty-acid desaturase which introduces a double bond at the 8-position in the long-chain base (LCB) of ceramides. Required for the formation of the di-unsaturated sphingoid base (E,E)-sphinga-4,8-dienine during glucosylceramide (GluCer) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>an N-acylsphing-4-enine + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = a (4E,8E)-4-sphinga-4,8-dienine ceramide + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:46280"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:85953"/>
            <dbReference type="EC" id="1.14.19.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.18" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714795.1">
        <property type="nucleotide sequence ID" value="XM_018858649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJH7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJH7"/>
    <dbReference type="GeneID" id="30031625"/>
    <dbReference type="OrthoDB" id="260091at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016717">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03506">
        <property type="entry name" value="Delta6-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF30">
        <property type="entry name" value="DELTA 8-(E)-SPHINGOLIPID DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353">
        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015921">
        <property type="entry name" value="FA_sphinglp_des"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="9 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="20">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="92"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00016939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA24314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="546" mass="62367" checksum="6670CE5AE88FF88B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJJ5</accession>
    <name>A0A1A0HJJ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal protein 60S</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_77151</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714815.1">
        <property type="nucleotide sequence ID" value="XM_018858652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJJ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJJ5"/>
    <dbReference type="GeneID" id="30031628"/>
    <dbReference type="OrthoDB" id="1227494at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001859">
        <property type="entry name" value="Ribosomal_P1/P2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF103">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00456">
        <property type="entry name" value="RIBOSOMALP2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="11750" checksum="0B19313C23CECEB4" modified="2016-10-05" version="1">MPLLTPPVKYLAAYLLLVNAGNTSPSAADVKKVLESVSIEVEEDKVEKLLAEVEGKNVEELIAEGNEKLSSVPSGAPAAAGSDGAASAGAEAAAEEKEEAAAEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJN0</accession>
    <name>A0A1A0HJN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_228734</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714578.1">
        <property type="nucleotide sequence ID" value="XM_018855214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJN0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJN0"/>
    <dbReference type="GeneID" id="30028190"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291832" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="22"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="220" mass="24285" checksum="13DCBC582BEB3FE5" modified="2016-10-05" version="1">MKLGIWAVAVVLSIFHSVALSQEITNHTWQIHPRETNLTDLTVLTDIKYTVINSPSVIVQGTFWNNGSFFVFNNERPIQLQVKGPGFHNFGTISFNSISYVEEASYAIWAGGAFWNSGTIYFDACKTPFEEVPFIISSTRLWYNEGRMIFKKTAGSIGELFAGGRSVKDNSLAITNQGTISLYNTHWGVQTNINGNGCIVVGSGSRLNLDFASGAPRLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJN4</accession>
    <name>A0A1A0HJN4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_117841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714583.1">
        <property type="nucleotide sequence ID" value="XM_018854086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJN4"/>
    <dbReference type="GeneID" id="30027062"/>
    <dbReference type="OrthoDB" id="4090091at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="370" mass="42111" checksum="5956A4E3EE1794AB" modified="2016-10-05" version="1">MVLNKSKWDHKAKIQYLRKHNLTRPKPTANVNRPKWSAKKSQGSQGTAWLDEEDDSDWDLEDEAFLNHFYPQISEDHLSVENKQKLKRQIIKIVKARENGEPEVQEHASDEEDGIYLGARPVLHEAESFPEDEEDAESGEEYLLEIADLETKLSEFVVSDMGKLGNRKMLKNKILDNLLDEYGLDSYASTVKTTDYNNSAKLQFRNVDRLTAADLHGFRIGGPKNEPKQPSVQALDATELEAHRARAEKLRHVKFHDQIKKKFGDEPAKKTRVLEINNFNANDEMQMAALNSRLTLDNGALHRATSMDEDLDVLLGLESGGQTQQPPLESLDFLQDTLPWAGSLKREPARKETKKKESHTDSFLDDLLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJS8</accession>
    <name>A0A1A0HJS8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_65811</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714628.1">
        <property type="nucleotide sequence ID" value="XM_018858357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJS8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJS8"/>
    <dbReference type="GeneID" id="30031333"/>
    <dbReference type="OrthoDB" id="3886144at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF15">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="829" mass="93300" checksum="D8D3A01FE20C7A62" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX1</accession>
    <name>A0A1A0HJX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RhoGAP-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_77261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714955.1">
        <property type="nucleotide sequence ID" value="XM_018858667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX1"/>
    <dbReference type="GeneID" id="30031643"/>
    <dbReference type="OrthoDB" id="437889at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010447">
        <property type="term" value="P:response to acidic pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF128">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN RGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="11"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="402"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="340"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="340"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="600" mass="67535" checksum="43A65ED1F9422C42" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK19</accession>
    <name>A0A1A0HK19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_30485</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714634.1">
        <property type="nucleotide sequence ID" value="XM_018855647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK19"/>
    <dbReference type="STRING" id="869754.A0A1A0HK19"/>
    <dbReference type="GeneID" id="30028623"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000637">
        <property type="entry name" value="ATP-dependent RNA helicase DHX37/DHR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="441"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="702"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="179"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="726"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="975"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="276"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="743"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="976"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1278" mass="142015" checksum="AC553B96FEEBC868" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKC8</accession>
    <name>A0A1A0HKC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_10491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714823.1">
        <property type="nucleotide sequence ID" value="XM_018853779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKC8"/>
    <dbReference type="GeneID" id="30026755"/>
    <dbReference type="OrthoDB" id="10681655at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="137" mass="16291" checksum="55346677B2F6BCC1" modified="2016-10-05" version="1">MSESLKELDLLQSPVFVTETFTQRARDSGYFETKSWEDLLQCFKSAIMKMFDEFLKVKESLVSLQTGSACGMNFIVDSIKYIREPKNEILKCYFQFYELNNPQGVFGFTDINEYTYVKTYLRKSTWYCEVDMDNFWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKK9</accession>
    <name>A0A1A0HKK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_34146</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714908.1">
        <property type="nucleotide sequence ID" value="XM_018856446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKK9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKK9"/>
    <dbReference type="GeneID" id="30029422"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032435">
        <property type="term" value="P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="31"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="211" mass="22620" checksum="857D2079B0AE128C" modified="2016-10-05" version="1">MTSNSTQLATVGLAGFFGGYLLCRWLKQTEPVKRRPLPRAQPQARDSRDSLTTGSSTESSTESGSESASESDSEDDIEIDSTPLNEIPGEVRMTLVVRQDLKMGKGKAAAQCSHATLALYKKMATPSSQAYNPEMLHRWEYGNGQAKITLQVPNMEEMDVLFAQAISLGVNAYTVHDAGRTQIAAGSATVLGLGPAPKAVLDQITGGLKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKN4</accession>
    <name>A0A1A0HKN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_30722</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714933.1">
        <property type="nucleotide sequence ID" value="XM_018855691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKN4"/>
    <dbReference type="STRING" id="869754.A0A1A0HKN4"/>
    <dbReference type="GeneID" id="30028667"/>
    <dbReference type="OrthoDB" id="10250549at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903574">
        <property type="term" value="P:negative regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04652">
        <property type="entry name" value="LbH_eIF2B_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051960">
        <property type="entry name" value="eIF2B_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056764">
        <property type="entry name" value="LbH_EIF2B3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25084">
        <property type="entry name" value="LbH_EIF2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EIF2B subunit epsilon/gamma LbH" evidence="10">
        <location>
            <begin position="334"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="441"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="441"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="457"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="476" mass="50673" checksum="7745741819159C3A" modified="2016-10-05" version="1">MEFHAVILCGPGKQLAPFSQQRATGAPKALLPVGQRPMFEHVLDWCEKAFFPKVTLLCDAAAGAAVRDAVARYKAARAAAVALAPDVLDVASDVLRFLRGIDVAEHGAAASGQALQYVAQHAAPGPLAHFVVLPCDLVTDLPPHVLIEAYRCRRAADLGMLVCYRPRPGPEDKKHRAGPRSYTVYSGLPCGGRAQLLDYYSAADVAFHKGVAVRTQMLWRHATPAVSTRLANGAVFFGDARVLAARLRSVPAKFGDAYFALRPLIKVVRDLARREWQSPGWGLSVGLVEVPRQAVFLRANTLAGYMEATRHLLQRHARDTAGAKDRPAGAKDKAAAAVGADSMVGAASRLGDKTNVKRSVVGAHCTIGKRVRVTGSVVLDGAVVEDDVLLENTIVGRAATVRSRSRLTNCYVEATHDVPRGTHAKGDTLLCLTLEGLVEAAGDSSSSDDAASASSSDDYDSYDPDDGDNSDGLFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ30</accession>
    <name>A0A1A0GZ30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_14004</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709308.1">
        <property type="nucleotide sequence ID" value="XM_018854468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ30"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ30"/>
    <dbReference type="GeneID" id="30027444"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000006">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.10:FF:000004">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000052">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56037">
        <property type="entry name" value="PheT/TilS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="17">
        <location>
            <begin position="290"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA17011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="589" mass="66326" checksum="5B498BB9D2EBD3D4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Z1</accession>
    <name>A0A1A0H1Z1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47626</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709444.1">
        <property type="nucleotide sequence ID" value="XM_018858197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Z1"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Z1"/>
    <dbReference type="GeneID" id="30031173"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF6">
        <property type="entry name" value="TRANSCRIPTION FACTOR SEF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA18049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="637" mass="73117" checksum="7016C54D5D64864F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4J8</accession>
    <name>A0A1A0H4J8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="4 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_79962</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the sequential decarboxylation of four acetate groups of uroporphyrinogen-III (octacarboxyporphyrin) to yield coproporphyrinogen-III (tetracarboxyporphyrin) with the formation of intermediate hepta-, hexa- and penta-carboxylate porphyrinogens in the heme biosynthesis pathway. Acts on a number of porphyrinogens, but only coproporphyrinogen III can ultimately be converted to heme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="4 14"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709534.1">
        <property type="nucleotide sequence ID" value="XM_018859153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4J8"/>
    <dbReference type="STRING" id="869754.A0A1A0H4J8"/>
    <dbReference type="GeneID" id="30032129"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000004">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="17">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA18999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="361" mass="40901" checksum="ADD1ED59934A62B6" modified="2016-10-05" version="1">MTFAPLKNDLILRAAKGEKVERPPIWIMRQAGRYLPEYHEVKGKRDFFETCRDAEIAAEITIQPVDHFDGLIDAAIIFSDILVIPQAMGFEIEMVEGKGPVFVDPLRSPDDLKRVVMKPDIMKELDWAFKAITLTRTKLNGRVPLLGFCGAPWTLLVYMTEGQGSKMFRFAKQWIYEFTRESHALLQAITDACIEFLAQQVVAGAQMLQVFESWAGELGPREFNEFCLPYLRQIAQKLPARLTELGVSEKIPLTVFAKGAWYALDDLCESGYDTVSLDWLYRPEDAVKVVNGRRITLQGNLDPGIMYGSDEVISQKVEEMIKGFGGGKQNYIINFGHGTHPFMKPEKVGHFLKECHRFGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4U0</accession>
    <name>A0A1A0H4U0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone-fold-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_80036</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TAF9 family. CENP-S/MHF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709629.1">
        <property type="nucleotide sequence ID" value="XM_018859163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4U0"/>
    <dbReference type="STRING" id="869754.A0A1A0H4U0"/>
    <dbReference type="GeneID" id="30032139"/>
    <dbReference type="OrthoDB" id="1872155at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22919">
        <property type="entry name" value="HFD_CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029003">
        <property type="entry name" value="CENP-S/Mhf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980:SF0">
        <property type="entry name" value="CENTROMERE PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22980">
        <property type="entry name" value="CORTISTATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15630">
        <property type="entry name" value="CENP-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10506" checksum="3B9ADFB0A425D98C" modified="2016-10-05" version="1">MSDLPTQLKGSVYLGVAKMVEEHCQDLGITASPSFVASLVELVYNQILLLGEDLELFAEHAGRSTINTSDMYMITRKNEILATALKEYEKNLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Z1</accession>
    <name>A0A1A0H4Z1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative ribosome assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_33606</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709512.1">
        <property type="nucleotide sequence ID" value="XM_018856348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Z1"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Z1"/>
    <dbReference type="GeneID" id="30029324"/>
    <dbReference type="OrthoDB" id="10261640at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000630">
        <property type="entry name" value="Ribosome assembly protein SQT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF8">
        <property type="entry name" value="ANGIO-ASSOCIATED MIGRATORY CELL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA18977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="417" mass="46185" checksum="31DCC8BE692E6CE4" modified="2016-10-05" version="1">MSESEYQENEEHFNEEEFVDSNEIAEEVEDDFGPGPEEIDENDMDMDDEETLEIDLANNSWTYFDQHKDSVFVIFKHPSLPMVVTGGGDNTAFMWTTHTQPPRFVGELLGHKESVVAGGFTGDGKFVITGDMNGCIQVHQAAKGGQKWSRFGELEEVEEVLWISVHPHLPYFAFGAPDGSVWVYQIDESSKSLEQIMSGFSHNSECNGGVFVNTDDNESLTLVTISEDGSIVNWNAFSGATNYKLQPHDDFKGVESPWVSIKTYKNVFAVGARDGQLAIVNLDTGRIVHHVKTIENVEDIADLSVEALSWCKSPTVNLLAVGLVYGDVLLFDSLQWRLRRTLKADDTITKLEFVDDKPILVGSSMNGKIYKWDARTGEELFVGVGHNMGVLDFAVLDGGAKLVTAGDEGVSLVFVHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H509</accession>
    <name>A0A1A0H509_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_219775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709696.1">
        <property type="nucleotide sequence ID" value="XM_018855123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H509"/>
    <dbReference type="GeneID" id="30028099"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="75" mass="8832" checksum="FC25EB045D3F1DE9" modified="2016-10-05" version="1">MYEHARLNFIRRHPRVSLADSWYANYPVLYTRHKTLNEPSSGFAAHTHSPPFLAHSTCFFLFPTLLSFIFPRFCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B2</accession>
    <name>A0A1A0H5B2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">37S ribosomal protein S9, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47357</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709632.1">
        <property type="nucleotide sequence ID" value="XM_018858154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5B2"/>
    <dbReference type="GeneID" id="30031130"/>
    <dbReference type="OrthoDB" id="10254627at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000001">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_uS9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001099">
        <property type="entry name" value="PRK00132.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="281" mass="31375" checksum="7A9D5EE161EADD38" modified="2016-10-05" version="1">MSSDRPSVPLNAVKVSRFSASHVTTRFHDWELERMRVVPTSSAFYGGNPVHDDNMYKLNAMIKKYKDLPTRVIDIKESNLLKFITLAEYKKLANSGARVKVGHHKALVGALGRLRSIQPELMPAEVSELLKQYLSSGGDTSKELQKKIKTLDEFGRALTVGKRKTSVASVSMVKGDGQVLINGRSFIEYFPRDTDRLKIAFPFKVVSQEGQYNIFVETHGGGVSGQAEATMYGIAKALVVFNPLLKPRLREAGLMTRDSRVVERKKPGKVKARKSPAWVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5C3</accession>
    <name>A0A1A0H5C3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proline-specific permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_219563</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709682.1">
        <property type="nucleotide sequence ID" value="XM_018855122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5C3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5C3"/>
    <dbReference type="GeneID" id="30028098"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF39">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="466"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="56"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="570" mass="63773" checksum="C201DD948B5880BA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5G4</accession>
    <name>A0A1A0H5G4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">beta-glucosidase</fullName>
            <ecNumber evidence="3 6">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33396</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; cellulose degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3 6"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709717.1">
        <property type="nucleotide sequence ID" value="XM_018856282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5G4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5G4"/>
    <dbReference type="GeneID" id="30029258"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="UniPathway" id="UPA00696"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.300:FF:000006">
        <property type="entry name" value="Beta-glucosidase H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000495">
        <property type="entry name" value="Periplasmic beta-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="entry name" value="Glycoside hydrolase family 3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00758">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="Beta-D-glucan exohydrolase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="410"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="846" mass="92846" checksum="C18C0B0FFE7A98EE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5J4</accession>
    <name>A0A1A0H5J4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flo11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_219956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709720.1">
        <property type="nucleotide sequence ID" value="XM_018855126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5J4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5J4"/>
    <dbReference type="GeneID" id="30028102"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291712" description="Flo11 domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="285" mass="29240" checksum="FBAF4185D18CC5B3" modified="2016-10-05" version="1">MMTNFGLPLLLLLLAAHSALAASSDITYTVTSSVIEIIPVTKYIFTTDGTLTSTLVTGTGLYIAATETSSSSTKAVLTDVAVEPSSNEVVEPSSSASASVTLALPLVSITASPLPIETSTESSAVKTSTVETSTVELSTIKPSTAKSSIAESYVAESSTAEASSAQTSTTEKSSVVSLSATTLKSSMETPVVSSTETSILSTKSPYEPSFPDSSVPVPSSGIATVTVSETSGCPAQTACEAPAFTPTKPVIPSGLYSTSYYTTTEKVNGAWAVVEYEVLNTFVCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5J9</accession>
    <name>A0A1A0H5J9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_33405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709725.1">
        <property type="nucleotide sequence ID" value="XM_018856286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5J9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5J9"/>
    <dbReference type="GeneID" id="30029262"/>
    <dbReference type="OrthoDB" id="5405745at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024338">
        <property type="entry name" value="MID1/Yam8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142:SF1">
        <property type="entry name" value="AEL197CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39142">
        <property type="entry name" value="MID1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12929">
        <property type="entry name" value="Mid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="518" mass="57853" checksum="EC4C6377EB6D2D41" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5L1</accession>
    <name>A0A1A0H5L1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DUF676-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_46901</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709863.1">
        <property type="nucleotide sequence ID" value="XM_018858099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5L1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5L1"/>
    <dbReference type="GeneID" id="30031075"/>
    <dbReference type="OrthoDB" id="5368485at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016445">
        <property type="entry name" value="Rog1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005412">
        <property type="entry name" value="UCP005412_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="4">
        <location>
            <begin position="174"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="423"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="425"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="703" mass="78927" checksum="5F38279B7592C77F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5L7</accession>
    <name>A0A1A0H5L7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peroxisomal membrane protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709740.1">
        <property type="nucleotide sequence ID" value="XM_018858082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5L7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5L7"/>
    <dbReference type="GeneID" id="30031058"/>
    <dbReference type="OrthoDB" id="39659at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019531">
        <property type="entry name" value="Pmp4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15460">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15460:SF3">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF013674">
        <property type="entry name" value="PXMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="230" mass="26694" checksum="8EA35C52A53A3587" modified="2016-10-05" version="1">MTILTDPFFRPLLEIVQSSRNGVVYGGKIRFSHALVINLLYRSGPLVPRLKQVFKATKDHSAVLAAFATIYKTVVNLLKKEQLLSRRPSFVKFVAGAVAGWVVYSQHFGYFNPGITHQITLYCFSRVLVAACKIFLDLYLQCRQPMFTVKGEAKNYADLTPNQSKRYKAKVYNKTWKYFAVLTWALVMFIYDYQPEYLQSSLRHSMAYIYDVEMDTWASWKEFLGIHRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5L9</accession>
    <name>A0A1A0H5L9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79897</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709921.1">
        <property type="nucleotide sequence ID" value="XM_018859145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5L9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5L9"/>
    <dbReference type="GeneID" id="30032121"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015191">
        <property type="term" value="F:L-methionine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903692">
        <property type="term" value="P:methionine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF382">
        <property type="entry name" value="LOW-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="455"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="512" mass="57326" checksum="D2F0179E96E1467E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5N2</accession>
    <name>A0A1A0H5N2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA/RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709744.1">
        <property type="nucleotide sequence ID" value="XM_018856290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5N2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5N2"/>
    <dbReference type="GeneID" id="30029266"/>
    <dbReference type="OrthoDB" id="69928at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018834">
        <property type="entry name" value="DNA/RNA-bd_Est1-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10373">
        <property type="entry name" value="EST1_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA/RNA-binding" evidence="2">
        <location>
            <begin position="99"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="464"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="743"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="781" mass="88355" checksum="48E43D0317C82B96" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5T9</accession>
    <name>A0A1A0H5T9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_221098</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709804.1">
        <property type="nucleotide sequence ID" value="XM_018855140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5T9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5T9"/>
    <dbReference type="GeneID" id="30028116"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="134"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="375" mass="42130" checksum="8E5FEB2321B26211" modified="2016-10-05" version="1">MRNVRGSEPIIFVSQFSLSGCPITHAHLRLLAQHIYQIMGYPQRISGINFKPSSSYRRFSLCFSTNTFTYCTRFSNPPNSLIKIASYSSFAELSCDFTGFKRIVANRQPKIDFKSQIIRKMHITPIPSSWGESNNYAYLIVDDSTRHGWLIDAAFPTDVLSYLAEKKPNVELKAIVNTHHHWDHAGGNRTFHKKYPDLPIIGGKDSELVTYTPSHQEVIDLGDNISITALHTPCHTQDSICYYAKDSKTGEKAVFTGDTLFISGCGRFFEGNAAEMNKALNEVLAKLPSETVVYSGHEYTKSNVRFSEKVMQNKALSELKALAESTEFTAGKSTIGDELKFNPFMRLSDPQVQKATAETDPVQVMAKLREMKNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5U5</accession>
    <name>A0A1A0H5U5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709987.1">
        <property type="nucleotide sequence ID" value="XM_018854322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5U5"/>
    <dbReference type="GeneID" id="30027298"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="473" mass="51705" checksum="0B399810F33EB944" modified="2016-10-05" version="1">MIKDKSGPRVAGQLTDSSSPSNITPATYGKPALHKKRKYRLAFAKAEKVLMGTVPKKQPLSILDKLGQIFGCTVRGDLSSILTANTEQTLVSKEDDRDVNVSAKPGNQPENTTTSDRISLKDELRLDGSPILEPEKYVEFSSLHMGSLAETSTPIKKTALTLRTGDGPKKRPIDVTALNLDSETHRKSSLETPPLGIKEYKSGSPRVAAKKAEKQHEPLETPVGVDTIFQEVLEMLIKGISELTGAGVGILISVNKASGEDHLLSRSARVNKKKRRKPARASDDCIKKYGKAHKKRRHKRRKYKVSEKNEAKNECASAHDEFSCLEAGTPRAINDCEKQAEATKKSNNEPHLITPLQAQESKKESGADDISSINLTVGLGANQSMEITGDKIMMQPALVPAYNTPDGEHPGTPLEGDERGPSLSPCQEISKQSENSQVKLFCQQKYRKMLAQCASGPDPIDLLRKNKNRGSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5U8</accession>
    <name>A0A1A0H5U8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_25096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709862.1">
        <property type="nucleotide sequence ID" value="XM_018855287.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5U8"/>
    <dbReference type="GeneID" id="30028263"/>
    <dbReference type="OrthoDB" id="3989460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="92" mass="10473" checksum="98194EF8B21C8F1E" modified="2016-10-05" version="1" fragment="single">DFHPVELAAHFLLSGSLDSLHETFLLLHESQKILLTRLHLIETRMTKVLENLDSSELDLAGVQTRVKNIRKKLQNCAVLVDKVEAKLETLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Y9</accession>
    <name>A0A1A0H5Y9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pkinase-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHEK2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709854.1">
        <property type="nucleotide sequence ID" value="XM_018859137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Y9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Y9"/>
    <dbReference type="GeneID" id="30032113"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FHA" evidence="6">
        <location>
            <begin position="48"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="142"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="473" mass="53039" checksum="DB2DABB5210D6D5A" modified="2016-10-05" version="1">MSLTRKRQLLEVQSLPKRQLHKYEPVAKLFSVKPDQPHLLIPKIPRGIVVGRSSSCDIKITGGDVSSKHCQFSLSSTNGQEFLLVTDTSTNGTFVNGELLGKGISALLRSGDKISFARSSTYIFRYVGDELGNAKKSFFDDYILQNQLGTGHYAVVREARDRTTGDIVAVKIFHPNKTKSGGINPDGGEEAKLRQEMNLLLSTNHPNIVRFISHYVEPINEFSSTTYLVLEKVNSGELFQRIINKLKLRQDETKAIYNQLLSGLHYLHGKNIIHRDIKPENILLDITPRSASNSNQTGPWDDDEYDVRVKIADFGLAKFIGELKFTNTLCGTPAYVAPEVLNNNQNYTTKVDIWLAGVLLYVCLCGFPPFSDELGPPSMREQILGGKFAFYSPYWDDIKDSALDLISNLLLVDPLQRYDISQSCQHFWFSENDDDIKHSFSSSMSNNSLQPVTSATAPITLKQRFTSELNFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6F0</accession>
    <name>A0A1A0H6F0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional adapter 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_217335</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710134.1">
        <property type="nucleotide sequence ID" value="XM_018855101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6F0"/>
    <dbReference type="STRING" id="869754.A0A1A0H6F0"/>
    <dbReference type="GeneID" id="30028077"/>
    <dbReference type="OrthoDB" id="270417at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001786">
        <property type="term" value="F:phosphatidylserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071470">
        <property type="term" value="P:cellular response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010520">
        <property type="term" value="P:regulation of reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000115">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.90:FF:000008">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016827">
        <property type="entry name" value="Ada2/TADA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025024">
        <property type="entry name" value="Transcriptional_adaptor_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="11">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="13">
        <location>
            <begin position="62"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="10">
        <location>
            <begin position="67"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="SWIRM" evidence="12">
        <location>
            <begin position="352"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR025024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="440" mass="50649" checksum="838235570D891898" modified="2016-10-05" version="1">MEKGRLYHCDVCSSDCSNRIRIKCAICTDYDLCVPCFASGSTTLDHKPWHDYQIIEQNTYPIFDKDWGADEELNLIQGCETFGLGNWQDIADHIGHRSKDEVALHYTKIYLDSKEYPLPEMDKDFSQISAGGFLKNRKARLEERKMMPLPPPKPKPAVSVPLCHELQGYMPGRLEFDHEAENEAEVPIKDMIFDPEDSAGDIELKLTILDIYNSRLTTRAERKRVLIANGLLEYRRNISNDKRKSKEEKEFMKRINAFVRIMTPQDFEDFSTDLLTELRCRMRIHQLQTWRRNGITTLEDGNKFEKDKLIRLAHYQRMGNGLGLAARTAALLLASNGVRKLSTPQPPYKPKINTSNVRAPLDLSHATDFDLLSAEEKQLCSALRILPKPYFAIKTQLMKEAIKNNGVLKKKDARQFLKIDVNKASKIYEFFVHMGWCSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6W6</accession>
    <name>A0A1A0H6W6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_218813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710223.1">
        <property type="nucleotide sequence ID" value="XM_018855113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6W6"/>
    <dbReference type="GeneID" id="30028089"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="156" mass="17601" checksum="35EB03D0894A73FB" modified="2016-10-05" version="1">MPPKRIPGILNVYCGNRLVRWSRARRWNERRTKLTSRWYITPPGALTCTEQVMSQPWFRATLTDFGLRLVFGFNALVHACPSMCSPFLVLFIFCTPAQPLAGCVRPVGPINFSFFRSAASPISFVCPRPSFLCAPSVLLSYGSIWLHPSHLLRTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H702</accession>
    <name>A0A1A0H702_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710206.1">
        <property type="nucleotide sequence ID" value="XM_018857983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H702"/>
    <dbReference type="STRING" id="869754.A0A1A0H702"/>
    <dbReference type="GeneID" id="30030959"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00083">
        <property type="entry name" value="bHLH_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="76"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="47472" checksum="7427593DD4616062" modified="2016-10-05" version="1">MQLSAPPKLKTTIIQLNKDIDPEDNNYEVLSNDYASDTKNSLVSFSLGVNEEQPRKKRRRSSSTIGEDELAKRRTETKQLHSMIEKRRRIKINREFEALKYLIPACRTADHSSRRVSAASNNANKIDGMYKLTILKTAVEYILYLHHILQKQHSLLSSSMEDFEFDISFADIPLDVNQYRNIDQEFDFHEFASRCASQSDNVDRQATFMSQNLNSTALSIAETEEGAEEANTEAKAAVGADYISWNSRIDQCTDQLTATQEAAEISPILTMLGKHKSEDNGQPRLSSSICSTNLQTYQSFLFANPSGHSNDTSTSTSPFTIPIKTLIKKNMFSLPDPALTSTNARCCGLISPESVSMNNDVMGQGQAYPRKMYFKSQVPPNNLIANVDGQPGHLELSNTEVDKLENASRMLLLLRKPSIGQLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H719</accession>
    <name>A0A1A0H719_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_44524</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710227.1">
        <property type="nucleotide sequence ID" value="XM_018857806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H719"/>
    <dbReference type="STRING" id="869754.A0A1A0H719"/>
    <dbReference type="GeneID" id="30030782"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF22">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="184"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="579" mass="63950" checksum="F59CF88C35C9258B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H753</accession>
    <name>A0A1A0H753_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_79157</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710325.1">
        <property type="nucleotide sequence ID" value="XM_018859010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H753"/>
    <dbReference type="STRING" id="869754.A0A1A0H753"/>
    <dbReference type="GeneID" id="30031986"/>
    <dbReference type="OrthoDB" id="5970at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061574">
        <property type="term" value="C:ASAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481:SF0">
        <property type="entry name" value="LD23870P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15481">
        <property type="entry name" value="RIBONUCLEIC ACID BINDING PROTEIN S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="174"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="189"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="219"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="298"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="33538" checksum="A2275CCEF6A6623B" modified="2016-10-05" version="1">MPQNTLYVTGFTRETKAADLAPDFEKYGELVRLDIPPPRSVDGEKFAFVEYKNSEDCEKALEIDGKSFPYALKDGLTVQLARSDPISSRRGFRGGLGGRGGYSGRGAGYMGRGGRGGYGYPSPYAPPRGGRGGYGYQNYGYERGGYGSGGYGGGYPSYPSRGGHDASYGSREPGYGGREPGYGGGDPAYGGRDASYGSAYPGYGGYAPAPYRDSYGYRGYDDYRAPGADDRDYGYLSRDKYHNPDRRRGADRNYDSSDRHALEGTHDSRDSDTRAFPSNEPEEYGRGRYSRSNSPKRDKSRSRSPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7G8</accession>
    <name>A0A1A0H7G8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_45402</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required to generate and maintain the structure of the tubular endoplasmic reticulum network and the vacuole. Induces high curvature in membranes and causes membrane tubule formation. Involved in membrane/vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710496.1">
        <property type="nucleotide sequence ID" value="XM_018857914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7G8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7G8"/>
    <dbReference type="GeneID" id="30030890"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180020">
        <property type="term" value="F:membrane bending activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990809">
        <property type="term" value="P:endoplasmic reticulum tubular network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032581">
        <property type="term" value="P:ER-dependent peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="18928" checksum="D320145587B3072D" modified="2016-10-05" version="1">MSLPPQVTSFLSSIDKSTSGVQALRVFEQQTGLPRSYAVLGGAGLYFVLVFLNIGGVGQLLSNIAGLVVPGYYSLIALESRGTADDTALLTYWVVFAFLSVIEFWSRAILYWVPFYFLFKTVFLLYIGVPSFGGASIVYESVIRPLSQAYIVKNKSVNKAVNDAAESVSTSVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7L1</accession>
    <name>A0A1A0H7L1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_45358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710407.1">
        <property type="nucleotide sequence ID" value="XM_018857910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7L1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7L1"/>
    <dbReference type="GeneID" id="30030886"/>
    <dbReference type="OrthoDB" id="1724687at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000554">
        <property type="entry name" value="Ribosomal_eS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047861">
        <property type="entry name" value="Ribosomal_eS7_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11278:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01251">
        <property type="entry name" value="Ribosomal_S7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00948">
        <property type="entry name" value="RIBOSOMAL_S7E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="187" mass="21157" checksum="2ECB1CEDA70919E6" modified="2016-10-05" version="1">MSAANKILSENPTELELQVAQAFTDLEAQTDLKAELRPLQFKSIKEFDVAGGKKALAIFVPVPSVAGYRKVQTRLTRELEKKFPDRHVVFLAERKILPKPARKSKKLQKRPRSRTLSAVHDKILEDLVFPTEIVGKRVRYLVGGAKIQKVLLDSKDSSAVDYKLDSFSQLYNKMTGKQVVFEIPGEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Q2</accession>
    <name>A0A1A0H7Q2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-specific glutamate dehydrogenase</fullName>
            <ecNumber evidence="4">1.4.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_44683</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NAD(+)-dependent glutamate dehydrogenase which degrades glutamate to ammonia and alpha-ketoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-glutamate + NAD(+) + H2O = 2-oxoglutarate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15133"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.2" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710453.1">
        <property type="nucleotide sequence ID" value="XM_018857827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Q2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Q2"/>
    <dbReference type="GeneID" id="30030803"/>
    <dbReference type="OrthoDB" id="184415at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004352">
        <property type="term" value="F:glutamate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006538">
        <property type="term" value="P:L-glutamate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val/Trp_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056365">
        <property type="entry name" value="NAD-GDH_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016210">
        <property type="entry name" value="NAD-GDH_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055480">
        <property type="entry name" value="NAD-GDH_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606">
        <property type="entry name" value="GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11606:SF24">
        <property type="entry name" value="NAD-SPECIFIC GLUTAMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23147">
        <property type="entry name" value="GDH2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23152">
        <property type="entry name" value="GDH_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000184">
        <property type="entry name" value="GDH_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate/phenylalanine/leucine/valine/L-tryptophan dehydrogenase C-terminal" evidence="5">
        <location>
            <begin position="682"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1061" mass="118734" checksum="41F62132DC37A7FF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U5</accession>
    <name>A0A1A0H7U5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_212073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710580.1">
        <property type="nucleotide sequence ID" value="XM_018855052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U5"/>
    <dbReference type="GeneID" id="30028028"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="93" mass="10911" checksum="E099A28B77FFAE67" modified="2016-10-05" version="1">MSLCQHCNKDRHQAQRIITLVWRWCSKGFELSRLLLFRFGEERLGCLKRKKIVQAVVKICLYEISCGIFLVLIGRNCCKCTYEWSCVAQLNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H822</accession>
    <name>A0A1A0H822_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Golgi apparatus membrane protein TVP23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_45061</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Golgi membrane protein involved in vesicular trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Golgi apparatus membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TVP23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710660.1">
        <property type="nucleotide sequence ID" value="XM_018857871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H822"/>
    <dbReference type="STRING" id="869754.A0A1A0H822"/>
    <dbReference type="GeneID" id="30030847"/>
    <dbReference type="OrthoDB" id="2151161at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008564">
        <property type="entry name" value="TVP23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13019:SF7">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05832">
        <property type="entry name" value="DUF846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="229" mass="25859" checksum="32BB7926B9D0FBF4" modified="2016-10-05" version="1">MSGYTQIESDIPESPILNDSGAYTAAPSVTNGSIPQPAVERTLLQRLKESSHPLALLFYIFFRIAPIFMYLFGNLFLALITKESRFILHFIILVLLISADFWNLKNISGRLLVGLRWWNEITAKEGTSEFENVWVFETADPDRYINPIDSKVFWTLLYVQPVAWGVLGIMAVLKLELLYLILIVISISLSLTNAVAFTKCDKFGKANLMANDFFSKATTGVFSRFNPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H878</accession>
    <name>A0A1A0H878_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_78642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710707.1">
        <property type="nucleotide sequence ID" value="XM_018858907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H878"/>
    <dbReference type="STRING" id="869754.A0A1A0H878"/>
    <dbReference type="GeneID" id="30031883"/>
    <dbReference type="OrthoDB" id="5138418at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303:SF2">
        <property type="entry name" value="COA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33303">
        <property type="entry name" value="CYTOPLASMIC PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="160" mass="17609" checksum="16414BE9AEA42647" modified="2016-10-05" version="1">MSMKPRIKAFFGPSREYAVVGASNNPSKFGYKILSWYVSHGLPVVPVNPREPEILGQTVVPSVPEILRALSEKRNVAHHQLAAADGLSISFLTPPQITVRTLDDISAVPHYKSLVKGLWFQPGSYDQQVLDKTEELGLCAVHQDECILVRGDEGLYAANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8D0</accession>
    <name>A0A1A0H8D0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription factor BYE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_71005</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negative regulator of transcription elongation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BYE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710803.1">
        <property type="nucleotide sequence ID" value="XM_018858393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8D0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8D0"/>
    <dbReference type="GeneID" id="30031369"/>
    <dbReference type="OrthoDB" id="79252at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46462:SF3">
        <property type="entry name" value="UPSET, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="10">
        <location>
            <begin position="62"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="11">
        <location>
            <begin position="140"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="306"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="562"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="625" mass="69216" checksum="221DFE5FE4D73962" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8M5</accession>
    <name>A0A1A0H8M5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor CEF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_197206</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CEF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710888.1">
        <property type="nucleotide sequence ID" value="XM_018854883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8M5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8M5"/>
    <dbReference type="GeneID" id="30027859"/>
    <dbReference type="OrthoDB" id="1410009at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140602">
        <property type="term" value="C:nucleolar peripheral inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047242">
        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45885:SF1">
        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="12">
        <location>
            <begin position="7"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="8"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="11">
        <location>
            <begin position="56"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="12">
        <location>
            <begin position="61"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="273"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="467"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="514"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="316"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="603" mass="64758" checksum="A757E1F9EF9DB233" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8N3</accession>
    <name>A0A1A0H8N3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin-95</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Karyopherin-95</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32365</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710877.1">
        <property type="nucleotide sequence ID" value="XM_018856039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8N3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8N3"/>
    <dbReference type="GeneID" id="30029015"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042564">
        <property type="term" value="C:NLS-dependent protein nuclear import complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061676">
        <property type="term" value="F:importin-alpha family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006656">
        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046822">
        <property type="term" value="P:regulation of nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060188">
        <property type="term" value="P:regulation of protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000027">
        <property type="entry name" value="Importin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="866" mass="95153" checksum="469CFC398C973EEB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Q1</accession>
    <name>A0A1A0H8Q1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_43849</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA. In the complex, it is required to stabilize and induce conformational changes of the catalytic subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat TRM82 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710784.1">
        <property type="nucleotide sequence ID" value="XM_018857705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Q1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8Q1"/>
    <dbReference type="GeneID" id="30030681"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106143">
        <property type="term" value="C:tRNA (m7G46) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106004">
        <property type="term" value="P:tRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03056">
        <property type="entry name" value="TRM82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028884">
        <property type="entry name" value="Trm82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288:SF0">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT WDR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16288">
        <property type="entry name" value="WD40 REPEAT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="427" mass="48250" checksum="C895F1B78CF20E58" modified="2016-10-05" version="1">MKHPFQIFVADQSGGHLFASVKNHLLAFRTSDGALIGSWADDVDLQTVQEKQLKAKREAEEKKELTDSGNEPVTKKAKTNKKEPKICPPGPGAPVIYNYLRCLALTRDEKHIIGTTDSDKAAVIFQIDYTQENCLSLIKRQVFPKRPCAVLTTIDDKSIILADKFGDVYEIGNDDSEAVPEKDLVPILGHVSMLSDVLVAEHNNQQFVLTGDRDEHIKVTHYPLSYVVKHWLFGHHEFVSCMHLPSFNKDLLISGGGDDYLLLWDWWASKQLSRVELRDLIEPHLTDAHFPPERFLQEDSPKEICVSRVLSITKNGFNLLFVLCENTNCILTFNIDENNSVTFKQKLLTKTPVVDVALVGDNLYAAVDVDSGEDLLEIFQINDDGVLEKQTSQLTAAISSHNKCEVSSREEFYPLYHIHTLRKRSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H943</accession>
    <name>A0A1A0H943_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Carboxypeptidase S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_57686</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711042.1">
        <property type="nucleotide sequence ID" value="XM_018858301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H943"/>
    <dbReference type="STRING" id="869754.A0A1A0H943"/>
    <dbReference type="GeneID" id="30031277"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000098">
        <property type="entry name" value="Gly-Xaa carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 18" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="542"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA20520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="575" mass="64495" checksum="B8A11C74C7C0CDB7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q2</accession>
    <name>A0A1A0H9Q2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar-sorting protein SNF7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Vacuolar protein-sorting-associated protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_204606</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711242.1">
        <property type="nucleotide sequence ID" value="XM_018854966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q2"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Q2"/>
    <dbReference type="GeneID" id="30027942"/>
    <dbReference type="OrthoDB" id="5592979at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070676">
        <property type="term" value="P:intralumenal vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="173"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="230" mass="25891" checksum="F105993237C648C8" modified="2016-10-05" version="1">MWSYMFGGNKQQKKELPKKAIVELREHIQMLNKKRSHLELQIADQDLLARKHIGTNKALAKNALKRKKGYEENLMKIENQIDSLETQLTAIESANLNLETMKAMKQGASAMKQIHGEYDVDKVETTMDDIREQVELADEISEAISRPVGTGYVDEDELDAELAALQAEDQQARELERLPAPKHAAPSAQSSRPVLALPEFPTVSKSQPETQDEEDEDEEALRALQAEMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9S1</accession>
    <name>A0A1A0H9S1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosomal protein 60S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32596</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711145.1">
        <property type="nucleotide sequence ID" value="XM_018856095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9S1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9S1"/>
    <dbReference type="GeneID" id="30029071"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05833">
        <property type="entry name" value="Ribosomal_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001859">
        <property type="entry name" value="Ribosomal_P1/P2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00456">
        <property type="entry name" value="RIBOSOMALP2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="82"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="110" mass="10899" checksum="B8378D99CD044F79" modified="2016-10-05" version="1">MKYLAAYLLLVQGGNTAPSAADISSLLSSVGVESEESRISSLLSELEGKSIEELVAEGNTKLASVPSGGAAASSGSAAAAGGAAAGEAVEEKAEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9X7</accession>
    <name>A0A1A0H9X7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase methylesterase 1</fullName>
            <ecNumber evidence="2">3.1.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_17948</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48548"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="3.1.1.89"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.89" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711327.1">
        <property type="nucleotide sequence ID" value="XM_018854750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9X7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9X7"/>
    <dbReference type="GeneID" id="30027726"/>
    <dbReference type="OrthoDB" id="194865at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022950">
        <property type="entry name" value="PPase_methylesterase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="36"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR022950-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="298" mass="32652" checksum="31D66895226244C1" modified="2016-10-05" version="1" fragment="single">KAALFPVQAAYTDPATGVCFNTYYAPAGSPAAPLYVCHHGAGSSAMTFWCLARQLRDLAEGDDVPGVFAFDARGHGGTCPPGLRYTLADLAGDFAFVMREFCERHRPGNPVCLVGHSLGGAVLTEFVARFPDLDCNVRGLVVVDVVEDTAVRSLLQMAVFLHKRPRAFGSYGQAIRWHLQTRLLRNEESARVSVPELLVRGPGGGLVWKASLLDMAPSWHTWFDGLSRKFLACRLTKQSLAKMLVLSSSETLDKGLTIGQMQGRFQLVVFNNSTNAGHFLHEDIPRQVAATLSEFMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA28</accession>
    <name>A0A1A0HA28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-aminobutyrate aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_32679</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer and homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711250.1">
        <property type="nucleotide sequence ID" value="XM_018856112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA28"/>
    <dbReference type="STRING" id="869754.A0A1A0HA28"/>
    <dbReference type="GeneID" id="30029088"/>
    <dbReference type="OrthoDB" id="10260828at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000029">
        <property type="entry name" value="4-aminobutyrate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="492" mass="53878" checksum="71BC59C7F694DBBE" modified="2016-10-05" version="1">MLRPRGFTALPRAWPSRAASSSVAAAYYPSEPVAPSLVTASVPGPKSIELNQHLGAVFDNRASYFVTDYGRSVGNYISDADGNQLLDVYCQISSIALGYNNPALLATAASPQMASALVNRPALACFPPADYGRILQDGLLAAAPPGTNKVWTALSGSCANETAYKAAFMYQAARRRGSLDFTDEELTSAMENQPPGAPDRAILSFDKGFHGRLFGSLSTTRSKPIHKLDIPAFNWPKAPFPALQYPLAEHVRENAEEEARCLAEFERIVRLHPPHSIAAIVVEPVQAEGGDNHASPAFFQGLRALTLEHDILMIVDEVQTGVGACGKFWAHEHWDLAVPPDMVTFSKKFQAAGFYFSDPALQPAQPYRQFNTWCGDPSKALLARTIYQEITRHDLVARTAQVGAYLYGQMEQMFARHPAAVHSLRGENFGTFIAWSCENPAKRNELLMRMRSVGVNIGGCGNHSVRLRPSLVFERAHADVFLEALEYSLRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA80</accession>
    <name>A0A1A0HA80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_42303</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711420.1">
        <property type="nucleotide sequence ID" value="XM_018857526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA80"/>
    <dbReference type="STRING" id="869754.A0A1A0HA80"/>
    <dbReference type="GeneID" id="30030502"/>
    <dbReference type="OrthoDB" id="431929at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057358">
        <property type="entry name" value="UBL_ZFAND1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677">
        <property type="entry name" value="ARSENITE INDUCUBLE RNA ASSOCIATED PROTEIN AIP-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14677:SF40">
        <property type="entry name" value="CDC48-ASSOCIATED UBIQUITIN-LIKE_ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25327">
        <property type="entry name" value="UBL_ZFAND1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="7"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="AN1-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="28954" checksum="B00691704CD6B511" modified="2016-10-05" version="1">MSALERAHDDQGIMDLGKNCAFCHRLDFLPFHCEFCKGTYCSAHRTLDNHECVGRRQKNAPKTAGIDRASNGPSAASLFPDRARHQKLLDALLESLGTAPARDRKTPLMKLTKFLHLQKLRRDAKKKSSFFGKLASRAVSATAEVAKIKKSAKGPASVNAADRVFMWVLYVNRNEEELEQISVETERRAVWVLKMWSVGRALDSIAETMNIMNHNNATQQSGERLNLFKAEKDEPVVLAASAKVSTSIPNGATLYLVKGAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA81</accession>
    <name>A0A1A0HA81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_167299</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711431.1">
        <property type="nucleotide sequence ID" value="XM_018854671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA81"/>
    <dbReference type="GeneID" id="30027647"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="180" mass="20796" checksum="1BDE870492D7E662" modified="2016-10-05" version="1">MISKDTTHRHGSWVVSFEEIGQPCSCTSPKKIAVLIDGKFLAGRVFWVMSMHLCRPLCHGHSNAICESGAARPQEFDLIYDCRSSDLEDNLHWSDFFFVKLEHCVIFSPIRRRAWNPANTRVFRGAPYTRMTTCKWCKNIEISLYQDFRLCRLLVPCDQGDLDSWAVLSQQKEQTMKSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG5</accession>
    <name>A0A1A0HAG5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711505.1">
        <property type="nucleotide sequence ID" value="XM_018857319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAG5"/>
    <dbReference type="GeneID" id="30030295"/>
    <dbReference type="OrthoDB" id="4024171at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="430" mass="49482" checksum="D65EBB48E28399B3" modified="2016-10-05" version="1">MESETASLESIALSEAETFIPETQPANGLRLSETYQENFRSYAPFLVSRRNIERYDSESEDDDRTMVSLPDGQTIMQRPSTTYNDLKVLVVALQKSNSQMYVFPDVRAFDTFKKNEGDRRKVRKISKSRHSNGQSIRSSPGEQPLRGRLPDSDLLTGISESGLPVLVVEVPHMSAFRPKTPYMIFRKYVKVRQSINSPPHGELTQFELSEFCAVKMKVFQHFKRYMFVFHPEEGPVFKVWAFQHNYRPFTDFNYKDTRFRVFGTSRSVSYPTFYNPELKLQVIDQDQSSLLDNLIEKKDEKNYRSGRKKHEVVDFGDLENPIPCPDNPIIKQVLENEYSSFLRNSVPQEMPPFGKFLAGSNSKGISMISKKYSEIGKIGVYQDPIEMEESNALSSSSTHLDSLVLSAVFLTLRETSLRSTVKRDELEIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAH3</accession>
    <name>A0A1A0HAH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_78107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711521.1">
        <property type="nucleotide sequence ID" value="XM_018858821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAH3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAH3"/>
    <dbReference type="GeneID" id="30031797"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="193"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="209"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="30395" checksum="674D22D0213C4DD2" modified="2016-10-05" version="1">MKQPTQPGPPPPLQDAVPPGASAALRHQGPFLGLPSHYAFDPNLYPLTNPPLYDPTMLPLGPQGGPRRRRISISNGQIGQIVNHEAFYLDDDSLDEYYDSAGGRPPAHAHAHAPLPHDDDAPLAPRPASHDHAVGGPLPLLQPAMLVLGPGSHAAPAPSSVAGVPPPNQTLVYNNEVIFNPHDGPIPGTAAWKKERLLERNRLAASKCRQRKKNAQQQLQSNMAKFERELQDKTERLGRAEKLLATYNAALQQHFAGDPSALDGLRPYVAQPPDNIKFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAI4</accession>
    <name>A0A1A0HAI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Zn-dependent exopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_42168</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711398.1">
        <property type="nucleotide sequence ID" value="XM_018857508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAI4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAI4"/>
    <dbReference type="GeneID" id="30030484"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000008">
        <property type="entry name" value="Glutamate carboxypeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="2">
        <location>
            <begin position="162"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="4">
        <location>
            <begin position="351"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="3">
        <location>
            <begin position="673"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="795" mass="89681" checksum="32CDFAC0CBAEF465" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAT4</accession>
    <name>A0A1A0HAT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_170508</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711502.1">
        <property type="nucleotide sequence ID" value="XM_018854687.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HAT4"/>
    <dbReference type="GeneID" id="30027663"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0180034">
        <property type="term" value="P:co-transcriptional lncRNA 3' end processing, cleavage and polyadenylation pathway"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
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    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00115">
        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15 18" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="232"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1504"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1542"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1513"/>
            <end position="1528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1542"/>
            <end position="1578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA20992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1592" mass="177554" checksum="463DA721E4BFB20D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAY6</accession>
    <name>A0A1A0HAY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_42234</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711558.1">
        <property type="nucleotide sequence ID" value="XM_018857516.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HAY6"/>
    <dbReference type="GeneID" id="30030492"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000481">
        <property type="entry name" value="Structural maintenance of chromosomes 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF172">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="14">
        <location>
            <begin position="616"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="495"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="777"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="867"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1091"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1314" mass="146386" checksum="68E6282B7B350D01" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB14</accession>
    <name>A0A1A0HB14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_11648</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711582.1">
        <property type="nucleotide sequence ID" value="XM_018854049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB14"/>
    <dbReference type="STRING" id="869754.A0A1A0HB14"/>
    <dbReference type="GeneID" id="30027025"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="51"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="162" mass="17870" checksum="540BF9C243A4A71D" modified="2016-10-05" version="1">MFRAATLLHRSLARIPRVSHSIASAGLQRTFTQSMARLHGHLHKAKPGEELKITFISKDGLQQTFEVAEGDNILDIAQAHNLEMEGACGGSCACSTCHVIVDPEFYDEIPEPDDDENDMLDLAFGLTETSRLGCQVKMTKDLDGIRIALPAMTRNLQSKDFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB84</accession>
    <name>A0A1A0HB84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="3">6.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Pantoate-activating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pantothenate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_11703</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-pantoate + beta-alanine + ATP = (R)-pantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pantothenate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711647.1">
        <property type="nucleotide sequence ID" value="XM_018854065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB84"/>
    <dbReference type="STRING" id="869754.A0A1A0HB84"/>
    <dbReference type="GeneID" id="30027041"/>
    <dbReference type="OrthoDB" id="2020436at2759"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004592">
        <property type="term" value="F:pantoate-beta-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00560">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000013">
        <property type="entry name" value="Pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.10">
        <property type="entry name" value="Pantoate-beta-alanine ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00158">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003721">
        <property type="entry name" value="Pantoate_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042176">
        <property type="entry name" value="Pantoate_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00018">
        <property type="entry name" value="panC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299">
        <property type="entry name" value="CYTIDYLATE KINASE/PANTOATE-BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299:SF1">
        <property type="entry name" value="PANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02569">
        <property type="entry name" value="Pantoate_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="34640" checksum="8F4E80F43B5BD64B" modified="2016-10-05" version="1">MTRAIQVLRTVQQVRQWRRQCLLNKESVGFVPTMGALHAGHCHLVHHSIKDNDRTVVSIFVNPSQFAPHEDLDAYPRTLDRDLAMLDEQFSEKPVDAVFVPKILELYPSGIVLEVEKQKGAFVTVQGFSEQLEGALRPAFFRGVATVVTKLLNVVTPDRVYFGQKDAQQCVVIKNLVKDLLIDTKVEILDTLREPNGLAMLSRNEYLSPETKDECTIIYQGLVAARTLFAKAQDEGMSVTAQAIREAIDKTYQNMPQDWKIDYVAISHPETLDELVTVDKDIGATVSVAVRVPKEDGTVARLIDNVQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBJ9</accession>
    <name>A0A1A0HBJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711762.1">
        <property type="nucleotide sequence ID" value="XM_018857330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBJ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBJ9"/>
    <dbReference type="GeneID" id="30030306"/>
    <dbReference type="OrthoDB" id="428895at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035426">
        <property type="entry name" value="Gemin2/Brr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04938">
        <property type="entry name" value="SIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="264" mass="29613" checksum="EC09C27E17273DF5" modified="2016-10-05" version="1">MSKRAVGANQNEPKKAKVLRALYTQGPLDEVFGQHRAFPLDAPSEEDSLVFEYLASVRKEAEHDQVCHFVSEEPGYSEEPYPAEDEVKDTPDLEASESPSLTEREAGTTESCSTEPKEKFVECGLQTLPDDPSNASYPVPASAASWRQLVFTTPVPSQEFFQNVLEHPTIIKLIVYYTKWLLGSLPESLSDWIFATFVRLDCDMDYQEMAIVRALGVKALKLRQKLFQGATEGAQISHTAQLCVDMVLAVISQYYGQKDLVRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC06</accession>
    <name>A0A1A0HC06_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MAP kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_56147</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711933.1">
        <property type="nucleotide sequence ID" value="XM_018858298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC06"/>
    <dbReference type="STRING" id="869754.A0A1A0HC06"/>
    <dbReference type="GeneID" id="30031274"/>
    <dbReference type="OrthoDB" id="1043025at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051403">
        <property type="term" value="P:stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06626">
        <property type="entry name" value="STKc_MEKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017240">
        <property type="entry name" value="MAPKKK_Ssk2/Ssk22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037579">
        <property type="entry name" value="MAPKKK_SSK22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="895"/>
            <end position="1172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1048"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="924"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1192" mass="136200" checksum="48CAF689CF7E8943" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC31</accession>
    <name>A0A1A0HC31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinetochore protein Sos7 coiled-coil domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_190743</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712204.1">
        <property type="nucleotide sequence ID" value="XM_018854840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC31"/>
    <dbReference type="STRING" id="869754.A0A1A0HC31"/>
    <dbReference type="GeneID" id="30027816"/>
    <dbReference type="OrthoDB" id="18959at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037475">
        <property type="entry name" value="Sos7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048781">
        <property type="entry name" value="Sos7_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37329">
        <property type="entry name" value="KINETOCHORE PROTEIN SOS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37329:SF1">
        <property type="entry name" value="KINETOCHORE PROTEIN SOS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20882">
        <property type="entry name" value="Sos7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Sos7 coiled-coil" evidence="3">
        <location>
            <begin position="60"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="206"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="338" mass="38340" checksum="F9A66347B4138AFA" modified="2016-10-05" version="1">MSRGSPTVRRQPQTTMNEEKGMFSVELAEDTYRAKVATSALKPSDPSLIKGDIASLKRFCSKLKFQYLEQDTRDKFVAVLFSEHAESVSQKNVDAVAGENTSAKAELKQLKKELAERVALSESVAEDVISINQRLQTRRKEAESASAELAALEKEFHALVSLPENESYKMLFDLNKMIDSQDVRPDEAIRIAETDLKQDQVMFHDLQQQEQALLEKAAQMNRHTEELQNTLLRMKTELEHSSRTPNGHPEPEQVRAQWLRELNSVLHKFTVAPVEIRQIGANYVLLCGRDEIVFSSDLNIVLVLNHVLRSLDVNQVNHVSGERKVEYLLRVIMKAFAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCC5</accession>
    <name>A0A1A0HCC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712037.1">
        <property type="nucleotide sequence ID" value="XM_018855970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCC5"/>
    <dbReference type="GeneID" id="30028946"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="141" mass="16280" checksum="EAA1A0C7EC6D42F6" modified="2016-10-05" version="1">MLYSRLGQDPLPAAPQPFPGNFKRAPKVPYKPVTFQSTEYWRHRKRPRLHNSHPYELPQRLGRAKQQKHPRARTKKTVGSEMSAAAPVRRPAHVQVPEEHPNAELTCRVYRASRRPSQINYPSATSRLLDRPQRHGPPISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCF0</accession>
    <name>A0A1A0HCF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_190545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712181.1">
        <property type="nucleotide sequence ID" value="XM_018854834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCF0"/>
    <dbReference type="GeneID" id="30027810"/>
    <dbReference type="OrthoDB" id="4083184at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="326"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="387" mass="43221" checksum="B476860ECC2A127B" modified="2016-10-05" version="1">MEFVISAVSITVRVCHRLILFPFTGVVFPIIRFFYGLVNHLLLIPIFLIFRTFIYGFIYIPLLPVLKVTEIHFDTLVPVELTLYRLAVGLLPHLHVFLYHSIHYFMVSMFVGTFVGMVAGFNISIVSRILCIGETKNESAVKRFGEAPVNAPEWKRVKRDPHSVVETSEISPKKQSPERPKHKNILEEVPKVKTEPNFMFSPTRESAELNGNAIFEDDDGYSFMTYKVSDDRRKHRKKDGFKKRRDNRYRSVPPRLQMTIEEESESETIPAPMHSTTAGIPIKHVSLAYATGTIGSSKTSSTDSSVFSKTLGQMSSETKEIALLDSPAGASDMSVAGADCKDKDDSGPKQDGYTRDILTSVHGPADADAPTADKVSAKEERSVSDAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCM4</accession>
    <name>A0A1A0HCM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoglycerate mutase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_70710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712251.1">
        <property type="nucleotide sequence ID" value="XM_018858389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCM4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCM4"/>
    <dbReference type="GeneID" id="30031365"/>
    <dbReference type="OrthoDB" id="10261749at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052765">
        <property type="entry name" value="PGM-Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46192">
        <property type="entry name" value="BROAD-RANGE ACID PHOSPHATASE DET1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="328" mass="38454" checksum="81A34EBF8BA30EDA" modified="2016-10-05" version="1">MGKPKYILLVRHGESEGNCNKLVNRYTPNHKICLTEQGHSQASQAGSVLKEFLTQLEEERDGGANPRSVMFYTSPYLRARQTCENIIKGIKDVPGVDYRVREETRMREQDFGNFQSTPEETEQIWEQRAHYGHFFFRIPHGESAADVYDRIASFNESLYRQFQNDSFPNVLVLVTHGIWARVFLMKWFRWSYEEFESLRNIPHCQFLVMKQVANKAYKLKTPLRKWDDLPDSDVDDEVAKEFTQEVTFNTKGNMNNPEDLDIASILRAQRDAIKENRKKDQELKNIYRQSMCKYLDCHKIVGDNSVFNSRENVPLYLKSSDEKSLIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS5</accession>
    <name>A0A1A0HCS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_12194</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712228.1">
        <property type="nucleotide sequence ID" value="XM_018854190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCS5"/>
    <dbReference type="GeneID" id="30027166"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF68">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="34"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="547" mass="60962" checksum="D0871D7D8C21B0DC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD30</accession>
    <name>A0A1A0HD30_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SPT2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_77408</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712344.1">
        <property type="nucleotide sequence ID" value="XM_018858694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD30"/>
    <dbReference type="STRING" id="869754.A0A1A0HD30"/>
    <dbReference type="GeneID" id="30031670"/>
    <dbReference type="OrthoDB" id="4035998at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013256">
        <property type="entry name" value="Chromatin_SPT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691:SF8">
        <property type="entry name" value="PROTEIN SPT2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22691">
        <property type="entry name" value="YEAST SPT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08243">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00784">
        <property type="entry name" value="SPT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="328"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="368" mass="40920" checksum="4BE320C5A813395C" modified="2016-10-05" version="1">MSASKILEQILRKGKITPVALANNSAGHTNDQQSTKPPRPNERPVDPVVARLKAARKAEREQKERELREKKGLPAKKATSTKPRIGPRQPARSLAQRTTPNNKSRTLHTTSNRLASAPPLQAQNTQNEKKPKMSFSQLMAKASGIDQSKMSIALKQKAKSSEVAGAPKRRTPEHDSRPDPASRKLQGLAGSLRNLSQESGRIPSLSARARSGNLAKAAGAPRAPHETQNSSQTRAPHPTRKPSSALAEKLKQKPRGKSQMLGHRAEVHDDESDESDMDSFIASEDEEMEQDEPEYDRDEIWSILNRGKKRSHYSYDDYDSDDMEATGAEILEEESQSKRNALLEDKREMEKEAKLAALKRARKSGIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDB9</accession>
    <name>A0A1A0HDB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]</fullName>
            <ecNumber evidence="10">1.3.1.88</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_77439</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.88" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712388.1">
        <property type="nucleotide sequence ID" value="XM_018858702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDB9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDB9"/>
    <dbReference type="GeneID" id="30031678"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102262">
        <property type="term" value="F:tRNA-dihydrouridine16 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102263">
        <property type="term" value="F:tRNA-dihydrouridine17 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="18">
        <location>
            <begin position="22"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="381"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA21892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="48526" checksum="C89905C808BF8212" modified="2016-10-05" version="1">MTVPKLTGRDLFNAIGQPKTIVAPMVDQLELAWRILLRRYGAQLCYTPMFHAKLFATDENYRRKMWSPWDGGDKDRPLIVQFCANDPEYLLQAAQHIETQCDAVDLNLGCPQGIARKGNYGAFLMDDWDLVYKLIRKLHENLKCPVTAKIRVYDDWEKTLEYARMVLSAGAQFITVHGRTREMKGQATGLANWKVLRYLRENLPKDQVFFANGNILYPSDLARCMSEVQCDAVMSAEGNLYNPGVFWTENADNDKVFARVDKMLREYFEIVKSCPGEASRVAMKAHFFKLLHAFLRVHTELRPVIGKTSVHADFEVWESIVEKVEKIVEEIYAKPDIQELDVITESELQPWGGKYKNVPYWRCQPYFRVVNGEKQNARTLKVAASDEVSENTGGKPQQEDEKKRAADDEAGAEAKRAKETEQGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDD9</accession>
    <name>A0A1A0HDD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 13">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8 13">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_10894</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="15">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate degradation; pentose phosphate pathway; D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 13">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8 13"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712486.1">
        <property type="nucleotide sequence ID" value="XM_018853876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDD9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDD9"/>
    <dbReference type="GeneID" id="30026852"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000171">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="12 15" id="KW-0170">Cobalt</keyword>
    <keyword evidence="11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="199"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA21990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="232" mass="24994" checksum="9E01DA528774F52F" modified="2016-10-05" version="1">MVQAIIAPSILASDFANLGCECRRMYALGADWLHIDVMDGNFVPNISMGPPVVASLRRHIPRKATNTFFDCHMMVACPEQWVPEIAQAGGDQFTFHYESTANPADLIKLIKLHGMRAACAVKPGTAADVVDELGALLDMVLVMTVEPGFGGQKFMAAMMPKVAQLRARFPHLDIQVDGGLGRDTVPVAADAGANVIVAGTSCFSAPEPAEFIAYMRDTVNARLREKGIYTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDF9</accession>
    <name>A0A1A0HDF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin biosynthesis protein CHS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_19705</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712459.1">
        <property type="nucleotide sequence ID" value="XM_018854880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDF9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDF9"/>
    <dbReference type="GeneID" id="30027856"/>
    <dbReference type="OrthoDB" id="245697at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17742">
        <property type="entry name" value="BRCT_CHS5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13945">
        <property type="entry name" value="Chs5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000077">
        <property type="entry name" value="Chitin biosynthesis protein CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031673">
        <property type="entry name" value="Chs5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052827">
        <property type="entry name" value="CHS_Export/Cell_Fusion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031669">
        <property type="entry name" value="Fn3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47351:SF1">
        <property type="entry name" value="CHITIN BIOSYNTHESIS PROTEIN CHS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16892">
        <property type="entry name" value="CHS5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16893">
        <property type="entry name" value="fn3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="164"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00060872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00071189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="279" mass="31273" checksum="811F2D38B5CD8826" modified="2016-10-05" version="1" fragment="single">MVEVSLTVGKLDASLALLLTKDHHLIEFPTVLLPNGVKAGSIVKISCERDLETEIEEHRRFLAVQDEILETFGKNLPQAPLLRIKNVTQTSCVLEWDPLDLGTATLKSLTLFKDGKKLGAIPQPLSNRTTKLSGLPIEKSFKFQLKLDSTAGVYTSDIVEVTTHKMTDLSGIQVCLGDIAPNDQFTLDDIQQALVNMGAHHPALDKMKVETTHFVTTRENKQNPEFMKANDMNIPIIRPEWLKACERERRIVGVRDFYVKDCVLPDILAKNYWLKPASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK6</accession>
    <name>A0A1A0HDK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Replication factor C subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_39957</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the activator 1 large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712473.1">
        <property type="nucleotide sequence ID" value="XM_018857210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDK6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDK6"/>
    <dbReference type="GeneID" id="30030186"/>
    <dbReference type="OrthoDB" id="446168at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA_replication_fac_RFC1_C"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="RFC1"/>
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    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
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    <dbReference type="PANTHER" id="PTHR23389">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="5 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="138"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="226"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="788"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="27"/>
            <end position="38"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="803"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="830"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020401"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR036578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="850" mass="91975" checksum="BB7BB736EBE89454" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDN9</accession>
    <name>A0A1A0HDN9_9ASCO</name>
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        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_39267</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018857121.1"/>
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    <dbReference type="STRING" id="869754.A0A1A0HDN9"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Tau138_eWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046488">
        <property type="entry name" value="Sfc3/Tfc3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035625">
        <property type="entry name" value="Tfc3-like_eWH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20222">
        <property type="entry name" value="DUF6581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="120"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor tau subunit sfc3/Tfc3 C-terminal" evidence="8">
        <location>
            <begin position="791"/>
            <end position="1177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="452"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="721"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="733"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1227" mass="137684" checksum="FB17EAD7BB3BB67B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDP1</accession>
    <name>A0A1A0HDP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor ISY1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_39875</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ISY1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712591.1">
        <property type="nucleotide sequence ID" value="XM_018857192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDP1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDP1"/>
    <dbReference type="GeneID" id="30030168"/>
    <dbReference type="OrthoDB" id="1739576at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009360">
        <property type="entry name" value="Isy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037200">
        <property type="entry name" value="Isy1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13021">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ISY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06246">
        <property type="entry name" value="Isy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140102">
        <property type="entry name" value="ISY1 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="228" mass="25435" checksum="E5D9CBD41534A1A2" modified="2016-10-05" version="1" fragment="single">LVACVSAMSRNKEKSQSGLHRYYEQKIQDAGVVRIDAGNRPRKVQSVETIAEAELWRRAVMSEMLAKMAAVNDPSLGADELRAANDALNRIYAEKRAWEHHILSLGGNDYIRYEKKIGTRVRGKTYFGRAKELPEARVGEKIHVGQGCSGQPGLSLAYYGVHEGALWDKSIDASKERVRAEINGAVGEKVFASAGMEAKLQEGGHSQKAVERWLVERKKRELMARLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE11</accession>
    <name>A0A1A0HE11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosomal RNA-processing protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_31182</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the RNA exosome complex. Specifically part of the catalytically inactive RNA exosome core complex (Exo-9) which may associate with the catalytic subunits RRP6 and DIS3 in cytoplasmic- and nuclear-specific RNA exosome complex forms. Exo-9 is formed by a hexameric base ring of RNase PH domain-containing subunits and a cap ring consisting of CSL4, RRP4 and RRP40.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712747.1">
        <property type="nucleotide sequence ID" value="XM_018855797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE11"/>
    <dbReference type="STRING" id="869754.A0A1A0HE11"/>
    <dbReference type="GeneID" id="30028773"/>
    <dbReference type="OrthoDB" id="437922at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030847">
        <property type="term" value="P:termination of RNA polymerase II transcription, exosome-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11370">
        <property type="entry name" value="RNase_PH_RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000004">
        <property type="entry name" value="Exosome complex component Rrp41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="155"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="27348" checksum="FC5F37600145A4AF" modified="2016-10-05" version="1">MSRMELYSPEGLRIDGRRWNELRRFECKINTHPNSSDGSSYVEQGNTKVMCMIQGPMEPPLRSQLNQTQATIEINLNIASFSTLQRKKRNKNEKRILELKTTLERTFEQSIMSSLYPKTVIEVNVQVLTQDGGLFSAMTNAITLALIDAGIAMYDYVSAVSVGIHDQTPLLDLNNLEENDMSCLTIGVVGKSEKLAMLLLEDKMPLDKLESVMGVAIAGGHRIRELMDNEVRGHGLMRLSKLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HED0</accession>
    <name>A0A1A0HED0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PDZ GRASP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31362</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712965.1">
        <property type="nucleotide sequence ID" value="XM_018855836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HED0"/>
    <dbReference type="STRING" id="869754.A0A1A0HED0"/>
    <dbReference type="GeneID" id="30028812"/>
    <dbReference type="OrthoDB" id="3318at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
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        <property type="entry name" value="GRASP65"/>
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    </dbReference>
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            <begin position="178"/>
            <end position="291"/>
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            <end position="460"/>
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        <location>
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            <end position="637"/>
        </location>
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    </evidence>
    <sequence length="914" mass="95799" checksum="1D37506C9FE5B341" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEU8</accession>
    <name>A0A1A0HEU8_9ASCO</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
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    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="EMBL" id="OBA22433.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
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</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
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            <taxon>Ascomycota</taxon>
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            <taxon>Pichiomycetes</taxon>
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            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
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        <location>
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            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OBA22500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="190" mass="21734" checksum="623D2EFCB102D46E" modified="2016-10-05" version="1">MSLPQISIEERLKAARQKFEELKKKNKIPQLRPSVLKSPTDIHTSGHVELKNDPEGHSVENPEHINELNETIELQKNQIKKLRDENTDLKLENMDLKDQVTELRNELEKLSARPSEPPFSQSISNKINTNEPLAQPDSQVLVVLTTNESQTNEEASASSNGDYKERVLAWKGWQVDMREWTGCQESQVVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF32</accession>
    <name>A0A1A0HF32_9ASCO</name>
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        <recommendedName>
            <fullName evidence="3">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
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                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018853977.1"/>
    </dbReference>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="3">
        <location>
            <begin position="380"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="468" mass="51080" checksum="240636D825F9C93F" modified="2016-10-05" version="1">MSVLRPPMYPNQLDRPFAPGRGHGKTASFGQLFSGLTSQRAARKVRSESPHHAVPPGPRAPKKRRSHDDGHATHGAPAGEPLPALKRPRTVPSLPISSRACSPMHVPVPPRAALAGSVTLPSLSDALGPMPDVAAGRKPSPKARPPTVSLDYFDTYMPNDEHWRFGLLDSIRHSKPAFSLDKYAYLNRHACGQPNLAAKPGPPAKPCFDSRTSCKAVPTFAQRKINFPYESSYTYLNSTYLHDVQKFPEYMELARLLVQLSRPPDGLQSPTTEACSLERLPTNAYSLERLPTNAHAHPQKPPSPLHAAHHVGSLSTARPLPLFSLGAHGTPQHSFEGRQHAPHAGSHVASHRTHFIPITPPSVKDKTRLELMKSPPRPAQGSLRICISCGTDLSPCWRPSWSCQEGQLCNSCGLRYKKTAARCLKAECKKIPAKGEWALMQSKGTTTYEDGKLAYGCLSCGGQVELKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF91</accession>
    <name>A0A1A0HF91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713147.1">
        <property type="nucleotide sequence ID" value="XM_018855372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF91"/>
    <dbReference type="GeneID" id="30028348"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="66" mass="7320" checksum="EF525E6458DBE938" modified="2016-10-05" version="1">MGKVSGSNPDSSNFFETRHLADEAEERLQNSLQACPELKTKCRSERPVAKLCSFTIYCICEKPGAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFB3</accession>
    <name>A0A1A0HFB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Homeodomain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_35052</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713151.1">
        <property type="nucleotide sequence ID" value="XM_018856564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFB3"/>
    <dbReference type="GeneID" id="30029540"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050891">
        <property type="term" value="P:multicellular organismal-level water homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901002">
        <property type="term" value="P:positive regulation of response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902584">
        <property type="term" value="P:positive regulation of response to water deprivation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902806">
        <property type="term" value="P:regulation of cell cycle G1/S phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032875">
        <property type="term" value="P:regulation of DNA endoreduplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000037">
        <property type="term" value="P:regulation of stomatal complex patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033993">
        <property type="term" value="P:response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000355">
        <property type="entry name" value="Transcription factor MYB124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="32"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="33"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="6">
        <location>
            <begin position="84"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="88"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16141" checksum="A191EE932A6FA60A" modified="2016-10-05" version="1" fragment="single">MSTEPPANQSENQHRTSFSEEEGPKQLDRTGPESLRRGPWSPEEDQKLMAIIALYGPLNWVRVSLLIKTRSPKQCRERYHQNLKPLLNRTPISYEEGLFIEQLVAKHGKKWAEIARHLSGRSDNAIKNWWNGGASRRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFC4</accession>
    <name>A0A1A0HFC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713093.1">
        <property type="nucleotide sequence ID" value="XM_018855858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFC4"/>
    <dbReference type="GeneID" id="30028834"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="73" mass="8559" checksum="C013CA3D86068477" modified="2016-10-05" version="1">MCSSAKLDFVGKTRYSVASRIQHNANTQMNIFFFTRELSILVFDRYNREISMQSSDSSTSHELKQNIVFMRIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFC5</accession>
    <name>A0A1A0HFC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Altered inheritance of mitochondria protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AIM11</name>
        <name evidence="8" type="ORF">METBIDRAFT_38490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the AIM11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713182.1">
        <property type="nucleotide sequence ID" value="XM_018857011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFC5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFC5"/>
    <dbReference type="GeneID" id="30029987"/>
    <dbReference type="OrthoDB" id="4088121at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038814">
        <property type="entry name" value="AIM11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39136:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="169" mass="18622" checksum="09E5213F62F9E56F" modified="2016-10-05" version="1">MSGTVEKSKLRDTLHKLNFKIADASPEYIARRKKQATFFLAAAAMTIFTSRFAHRTAISRQYIPSLFQGNHQPPLSYNFAMDAAVAVGTGTMLCGSVLSMIAFGACWVMDVSTLPEFGWRMKSLLGGYKSQQELEQVAIDEESKTIEDGINDLLEGNLDLGFDIVDESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFE4</accession>
    <name>A0A1A0HFE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein kinase CBK1</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_9077</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. COT1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713186.1">
        <property type="nucleotide sequence ID" value="XM_018859311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFE4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFE4"/>
    <dbReference type="GeneID" id="30032286"/>
    <dbReference type="OrthoDB" id="3638488at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000192">
        <property type="entry name" value="Serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF71">
        <property type="entry name" value="CITRON RHO-INTERACTING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="59"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="17546" checksum="AECCA0DC7B89B00E" modified="2016-10-05" version="1">MSVSHAIERNQRRLELESKLANEEAGMSEERKNRQLQNLGKKESQFLRLRRTRLSLEDFTTVEVIGKGAFGEVRLVQKRDSGKIYAMKTLFKAEMYKKDQLAHVKAERDVLAGSDSPWVVSLYYSFQDAVNLYLIMEFLIKRSHLEADPCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFI0</accession>
    <name>A0A1A0HFI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_92014</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713237.1">
        <property type="nucleotide sequence ID" value="XM_018859333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFI0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFI0"/>
    <dbReference type="GeneID" id="30032308"/>
    <dbReference type="OrthoDB" id="2261376at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001406">
        <property type="term" value="F:glycerophosphodiester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0843">Virulence</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="49"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="519" mass="57362" checksum="BF000B4D5C4F07B9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFT8</accession>
    <name>A0A1A0HFT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial aspartate-glutamate transporter AGC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Aspartate-glutamate carrier 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_29433</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Calcium-dependent mitochondrial aspartate and glutamate carrier. Transport of glutamate in mitochondria is required for mitochondrial transamination reactions and ornithine synthesis. Plays also a role in malate-aspartate NADH shuttle, which is critical for growth on acetate and fatty acids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713331.1">
        <property type="nucleotide sequence ID" value="XM_018855399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFT8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFT8"/>
    <dbReference type="GeneID" id="30028375"/>
    <dbReference type="OrthoDB" id="2161at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015183">
        <property type="term" value="F:L-aspartate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015292">
        <property type="term" value="F:uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000004">
        <property type="entry name" value="Calcium-binding mitochondrial carrier protein Aralar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051028">
        <property type="entry name" value="Mito_Solute_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678:SF9">
        <property type="entry name" value="CALCIUM-BINDING MITOCHONDRIAL CARRIER PROTEIN ARALAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45678">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="326"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="420"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="14">
        <location>
            <begin position="528"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="716" mass="80478" checksum="C74A04ABBFBC985C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFZ1</accession>
    <name>A0A1A0HFZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_9133</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713254.1">
        <property type="nucleotide sequence ID" value="XM_018859321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFZ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFZ1"/>
    <dbReference type="GeneID" id="30032296"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="375"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="593"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="156"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="780"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="937" mass="102421" checksum="BA9BFD503D246579" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG12</accession>
    <name>A0A1A0HG12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_9285</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018859352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG12"/>
    <dbReference type="GeneID" id="30032327"/>
    <dbReference type="OrthoDB" id="4084221at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="223" mass="26118" checksum="B50F9BE096782375" modified="2016-10-05" version="1">MSDDQLKQVRSAVNHVISNYQLTSKSKLFQRLSSKNVDLISKGVMENKQDKHLMELIKKRDYYTRKIHELLNDSGEEKNPRLIVDEAEAGHYIRKRLLKDVTRSEQIKSLIRKHRQFQVSATEEQDKIIQKYRVRKPLAGGLKKIGSMNAAIDAKLNAEREAELQRFYTNLMQKQSEYCLESERLLRNLDVPFFNLLLDDHSVTKSQKVFVLDLLYKVLAEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG14</accession>
    <name>A0A1A0HG14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_38171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713406.1">
        <property type="nucleotide sequence ID" value="XM_018856965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG14"/>
    <dbReference type="STRING" id="869754.A0A1A0HG14"/>
    <dbReference type="GeneID" id="30029941"/>
    <dbReference type="OrthoDB" id="2283785at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="90"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="781"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="910" mass="102699" checksum="B2C44CFC075370F9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG31</accession>
    <name>A0A1A0HG31_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal lysine N-methyltransferase 4</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_35924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that monomethylates 60S ribosomal protein L42.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SETD6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713294.1">
        <property type="nucleotide sequence ID" value="XM_018856671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG31"/>
    <dbReference type="STRING" id="869754.A0A1A0HG31"/>
    <dbReference type="GeneID" id="30029647"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1410.10:FF:000007">
        <property type="entry name" value="Ribosomal lysine N-methyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011383">
        <property type="entry name" value="N-lys_methylase_SETD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011771">
        <property type="entry name" value="RMS1_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="2">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR011771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="491" mass="56248" checksum="9A33F95F2BF7B2C5" modified="2016-10-05" version="1">MLFDERTLSFLTWLMQNAVTTSPKIRISDFRSSGQGRCVVATDEIMEDEELFSIPRSAILNVQSSSLAKEHAGAELVLAQMTQWDALVLVLLYEWKVKATSLPWWEYFNVLPLNDESYASNQLIYWDDTELSHLAPSYIVDRIGHNSANAMYDRLVPHFEKFVGTVTKDEFRKVASVIMSYSFDVGSKDVDDDEDNDEVSDDEVESNLVKDSDYLKSMVPLADTLNADTAKHNASLFITADHLVMRSIKPIAKNDQIYNIYAEHPNSEILRRYGYVEQQGLAHDFAEVSLATFKKYFAENSSLSKDTIDEILAILEEIEIEEDEKFVLESYDCFGSGEVIFELTFLTQLFVIIAAINHKKSFNSASLEVKARAIRRVYKKTYQLLESRRLTKQFMKDFPSILKVRLSEYPRKATRGFSPSRDTPLTRQEMAMAVLTSECVSLQNCMDTEKVFTKGEQEFSVIDDEKLLRNIMQKNTFEGSETQPSKKKRMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH44</accession>
    <name>A0A1A0HH44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713980.1">
        <property type="nucleotide sequence ID" value="XM_018856456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH44"/>
    <dbReference type="GeneID" id="30029432"/>
    <dbReference type="OrthoDB" id="4094692at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291929" evidence="1">
        <location>
            <begin position="20"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="30521" checksum="F12EEED8EABDBA65" modified="2016-10-05" version="1">MRYRSVCTGLLWLVALCSGASPLQNSLSSSCDLVSVQSYDEITSKYSDLLAPDSTRCVLFRIKESQTFLTRKGRRKWGLDTPVESLAKLGSLRQDSTQWLEFFYSDFANSTATGLTPVSLCHSEVLGEGSLVSFETIFNSASGFGGELTGDFDLFKLAVSTAAEVTLEKATSIAVKILCAIKPGEIGQLYLTNTQYIYYTPWFRTRGYDSVTNLFSDGDEYSERPRERRIMKGGIGDWVCASSSVADLQCHGTSGLRSLWNPGAASEQSEWRKDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH60</accession>
    <name>A0A1A0HH60_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_34905</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713816.1">
        <property type="nucleotide sequence ID" value="XM_018856535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH60"/>
    <dbReference type="STRING" id="869754.A0A1A0HH60"/>
    <dbReference type="GeneID" id="30029511"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110031">
        <property type="term" value="P:negative regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF196">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE SWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="563"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="590"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="937" mass="104832" checksum="0EA109382C266331" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH64</accession>
    <name>A0A1A0HH64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_35461</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713822.1">
        <property type="nucleotide sequence ID" value="XM_018856611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH64"/>
    <dbReference type="STRING" id="869754.A0A1A0HH64"/>
    <dbReference type="GeneID" id="30029587"/>
    <dbReference type="OrthoDB" id="190958at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="24052" checksum="0B45562A10DD5E49" modified="2016-10-05" version="1">MDKRNVANKASLVEPIIRHRVQDSLFYKQYLHLTNEQSILPVVAEHVQFVAGTDSSSRPSPFLCCLVRMLEIEPPIEIVRIYLDQNGYQEFKYLTALTLLYCRMVMGAADFYVLHDEYIKDYRKLRFKGKNPTVTDEIPVHYKIKHMDEWVDELATEERVVDIKIPYMGLRLVYIERGLVGEREYGAETSDDSEGEESEEYQSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH85</accession>
    <name>A0A1A0HH85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MPPN-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_63924</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713841.1">
        <property type="nucleotide sequence ID" value="XM_018858338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH85"/>
    <dbReference type="STRING" id="869754.A0A1A0HH85"/>
    <dbReference type="GeneID" id="30031314"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12721">
        <property type="entry name" value="RRM_Nup53p_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007846">
        <property type="entry name" value="RRM_NUP35_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21527:SF6">
        <property type="entry name" value="NUCLEOPORIN NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05172">
        <property type="entry name" value="RRM_Nup35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51472">
        <property type="entry name" value="RRM_NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6 8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM Nup35-type" evidence="10">
        <location>
            <begin position="187"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="387" mass="42035" checksum="A9F25821E8135A25" modified="2016-10-05" version="1">MASLFSLQETSKQIGAEPKQPLWYQNSARRVIPNHLVPRKRLAFQLSGTSTNKKDSKQSATNGASSNNNAFNSTDFNLISFGNTAHRTSSAGHGDSLSLFLTADGHDISQSFDTDDIPLYNENEDLPPAKSLYDLNDEVVISLNKPQQHTESFINKDPKTFVNAFHQKSDVSSTPEEDPAAKLSPLLISESAILVFGYPEAMTSQIIGYFSDFGTILEHFEALKNRRNENANILLAGTQTSSSSSGLPLFSGNSWVKLTFDNPASAQDALKESGSVFNGVLIGVVPYTKDAIEKLQKRKLSPLEDIGGGFQLLTRNISNEVDKGVAGDTNDLQASYIKRLDVKDGTGLFLKANGTGAQSEGLDEKGKGEKLGLLASVTKYFFGFHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH90</accession>
    <name>A0A1A0HH90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_76422</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713847.1">
        <property type="nucleotide sequence ID" value="XM_018858524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH90"/>
    <dbReference type="STRING" id="869754.A0A1A0HH90"/>
    <dbReference type="GeneID" id="30031500"/>
    <dbReference type="OrthoDB" id="1026733at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00166">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="UBX" evidence="3">
        <location>
            <begin position="332"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="438" mass="50713" checksum="B0354F67ACD1F96B" modified="2016-10-05" version="1">MDRIREVLNFPSIQRPLSNGPLNFPGHFPEAEIRETTAQLPYFNLLFVRPVTILVYLVLLLFFQVTQVLYFLGNSDSRSSLSAGTTASNALINDPISRAEHFVRELEENLLPLQQFTLYHSDRNSQYLPPFFQGSYTQALYMASTRAKFFYVYLTNLMSDGSQSLFAKVITNPKFRSIFQNEHVIIWGGDVTDPEAYQLANSLNITKFPMIGLLCLTRTTSMTPDGPRKSSPRISLILKIQGGLKDSTNPESLIHNKFVKRMAKYDGDLVIIRAELREKYLTEAMRHRQDADFQRSLLIDKQKKERKRLKKLSEKYLKWKQPYFKHLQESTEARGTLRIAIKFETGQRVTVMFPKNSPVEDIFLFVELHNRKMLDDMYETTMSDADAQALFRGFDMKFEFRLTSSIPPRPTLNDIHMQTPIEDVNFIFPSGLLMVERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHF4</accession>
    <name>A0A1A0HHF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_103972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713788.1">
        <property type="nucleotide sequence ID" value="XM_018853756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHF4"/>
    <dbReference type="GeneID" id="30026732"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="14306" checksum="E0EBF07B12F242D5" modified="2016-10-05" version="1">MKQTRDTVRNSSRVRIQISSIVIRKSKVRYRSARKMYLLPNSDTKEKKVCHQVSHNTFHDKAVLRYARNPTIQIQGSSYPKLVFIKTKKLSTKLQEVRLRENVTSLLMIFSLVLVSATLASHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHR1</accession>
    <name>A0A1A0HHR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic peptide chain release factor GTP-binding subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ERF-3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">ERF2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Polypeptide release factor 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Translation release factor 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_76542</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714026.1">
        <property type="nucleotide sequence ID" value="XM_018858548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHR1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHR1"/>
    <dbReference type="GeneID" id="30031524"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002184">
        <property type="term" value="P:cytoplasmic translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03704">
        <property type="entry name" value="eRF3_C_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04089">
        <property type="entry name" value="eRF3_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000017">
        <property type="entry name" value="Eukaryotic peptide chain release factor GTP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000503">
        <property type="entry name" value="Peptide chain release factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000061">
        <property type="entry name" value="Translation release factor eRF3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003285">
        <property type="entry name" value="Sup35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01343">
        <property type="entry name" value="YEASTERF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tr-type G" evidence="16">
        <location>
            <begin position="259"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="89"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="158"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="205"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA23545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="684" mass="75438" checksum="77320A98D05C2AF4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI13</accession>
    <name>A0A1A0HI13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="8">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_38422</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714003.1">
        <property type="nucleotide sequence ID" value="XM_018856999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI13"/>
    <dbReference type="STRING" id="869754.A0A1A0HI13"/>
    <dbReference type="GeneID" id="30029975"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="11">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="122"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="445" mass="48396" checksum="3B700F853140124D" modified="2016-10-05" version="1">MVKQVLLLGSGFVAKPTVDILAANANIEVTVACRTLAKAQQLAGSVAGAISLDVNDEQALDAAVAKVDLVISLIPYIYHELVVKSAIRNKKHVVTTSYINPKLQALEQQIQDAGITVMNEIGLDPGIDHLYAVKTIEEVHAQGGQIKSFLSYCGGLPAPENSDNPLGYKFSWSSRGVLLALTNQASYWKDGEVVNVKSEDLMATAQPYFIYPGFAFVCYPNRDSTTYKKLYNIPEAETVIRGTLRFQGFPEFIKVLVDLGFLKESAHPAFQAEAAWKDAFALLIGAASSSEKDLVAKIDLLASFKDEADRERIIAGLKWLGLFSANKTTARGNPLDTLCATLEALMQYEDGERDLVCLQHKFGIEWADGSKETRTSTLVDYGNPEGYSSMAKLVGVPCAVAVEQILDGTLSKKGLLAPMSSDINDPLMKTLKDKYGIFLVEKTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI77</accession>
    <name>A0A1A0HI77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_107458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714024.1">
        <property type="nucleotide sequence ID" value="XM_018853844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI77"/>
    <dbReference type="STRING" id="869754.A0A1A0HI77"/>
    <dbReference type="GeneID" id="30026820"/>
    <dbReference type="OrthoDB" id="4094935at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="257"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="420"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="580"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="722" mass="76936" checksum="CE6A31502E8BE7AE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI99</accession>
    <name>A0A1A0HI99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_64862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714207.1">
        <property type="nucleotide sequence ID" value="XM_018858350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI99"/>
    <dbReference type="STRING" id="869754.A0A1A0HI99"/>
    <dbReference type="GeneID" id="30031326"/>
    <dbReference type="OrthoDB" id="361870at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="entry name" value="Ribosomal protein L28/L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribosomal_bL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="Ribosomal_bL28/bL31-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribosomal_bL28_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL28M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="L28p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30589" checksum="941CFEDD6F89CB68" modified="2016-10-05" version="1">MPQPSFQTHTTSPMNFLRSLTAAAPVASSLFGMRCFGISAPTLREKYFKITKYARPIDTTVYKAGDKIPSKGVPTKNKLYPDYKYEAMFFKRQNRGLYGGLQRKRSKTCSESKNKNLRAHLPNIVKAKLWSETLNKCISTRVSTRLLRTVTKEGGIDNYLLKDSSAREKTMGLKGWRLRYDIMKTKELQERSAGSPKPVYHVLESGRKITVGKTKLLKHLYPFVYRDNYEPMDYEKFLRTHGYLTIAEVVSKLEHYKFDFNQVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIQ3</accession>
    <name>A0A1A0HIQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription factor TFIIIC, tau55 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_35053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714371.1">
        <property type="nucleotide sequence ID" value="XM_018856565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIQ3"/>
    <dbReference type="GeneID" id="30029541"/>
    <dbReference type="OrthoDB" id="414418at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000034">
        <property type="entry name" value="Transcription factor TFIIIC subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014623">
        <property type="entry name" value="Tfc7/tau55"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF51">
        <property type="entry name" value="TRANSCRIPTION FACTOR TAU 55 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036802">
        <property type="entry name" value="Tau55_TFC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="409"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="439" mass="48889" checksum="D2717210F84F5481" modified="2016-10-05" version="1">MAVSTILIARHGYRSNWLPPPHPPNPTGIDSDPVLAPHGVDQAIELSQYLGSLSENDRPQFIVSSPFYRCVETSQPVARALGLKVAIDRGVGEWFKTDRGVVPIPADYARLGQFFPEVIASEATWAGSGVIPSYKGETPDDIFRRCQQFCAEFLPKFEAQYPDVSTLLIVTHAATKIALGMSLLKHNSVNEEITFGGEKTKLRAGACSLDKYVRERESWVLKENGRTDFLKDGEEMNWNFDVKFEAGSDEDIKARNEAAKLAELSESPSKIDPETEEEAYEDLYFAIDVPVVAPSFYDELHRDNNRERDGLSDQLLINPNAKLQITELDHETPLYKISDNANVAADPESMLFSNLSAIDGQIYKTNWSRLLGTELIFDEYGELVGTVREHLVTDTSVKVKSKPSDKEANSQTETDTMDNSDLKTAFLKKAMAAARSKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIX3</accession>
    <name>A0A1A0HIX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714269.1">
        <property type="nucleotide sequence ID" value="XM_018853670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIX3"/>
    <dbReference type="GeneID" id="30026646"/>
    <dbReference type="OrthoDB" id="10587459at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="102" mass="11479" checksum="7AF4FB208EFB264C" modified="2016-10-05" version="1">MADLETPDASKEVAARRKTAAQKDHNRMFRQQQISSYKAAMLKEARAQRSRRRHGKGPENTAKLAAVLEPLEGSPKKRKKVNFNLQAPGREMALVLQKRGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ42</accession>
    <name>A0A1A0HJ42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_16178</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714502.1">
        <property type="nucleotide sequence ID" value="XM_018854630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ42"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ42"/>
    <dbReference type="GeneID" id="30027606"/>
    <dbReference type="OrthoDB" id="189997at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF3">
        <property type="entry name" value="HALOTOLERANCE PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="540" mass="61291" checksum="3064C594201A3EF3" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA3</accession>
    <name>A0A1A0HJA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA wybutosine-synthesizing protein 3</fullName>
            <ecNumber evidence="3">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_76967</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TYW3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714576.1">
        <property type="nucleotide sequence ID" value="XM_018858614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA3"/>
    <dbReference type="GeneID" id="30031590"/>
    <dbReference type="OrthoDB" id="263283at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000003">
        <property type="entry name" value="tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="13">
        <location>
            <begin position="7"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="268" mass="29993" checksum="9CFC18E6ED7882AA" modified="2016-10-05" version="1">MLSPFDQKKQLILREILGTSAAAPDASPKGTIDVLCISLIDTINSHPDMVTTSLCSGRVSVFLEGNKTKTQIGAKGHEGRWLFVTHDPESLLAWHESIDFAYEPMASPGLGENGEATRYILFKFEPLILHVKCRDSASANLLYSTAMACGFRESGIGSNHIVGIRILIKLDIPIGFLHGERLVLLVSSEYLGIITQLSLDRFKENFRKMDQLEAAIKQMRPVEIKAEETKEERRLRKIEEGMKRRESVRAEKEKKNRQKLEAEGGVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA7</accession>
    <name>A0A1A0HJA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MAGE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30287</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714404.1">
        <property type="nucleotide sequence ID" value="XM_018855601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA7"/>
    <dbReference type="GeneID" id="30028577"/>
    <dbReference type="OrthoDB" id="205198at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="1">
        <location>
            <begin position="40"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="292" mass="33127" checksum="63631C9CBA4E0763" modified="2016-10-05" version="1">MKMLKRTISAVEASCSSDLVNKRSATELPEIHEQEIVADIIRMILGKVSRNLRIRNEHIHSILQTLYSKGNLKYWISAVKKELEFTYGLSLEQCGSDITVFSNLKAHSREIFYSLCEANSPLVDYQANLLSSIFLIHKSKKDLMSINTSDKVMGGITMLIISIIVLSENRINENDLVNSLSAFGISSNLSIPVPLLNKPIQNILGDIAKRDYIKKTSPAGADQSGSYIEYSLGERCKREFDSQCIYNFFLQIYKEKDLQDKICESMKRCFPEFEPNLNEEATLNDMDLHIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJV4</accession>
    <name>A0A1A0HJV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RabGAP/TBC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_16248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714781.1">
        <property type="nucleotide sequence ID" value="XM_018854632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJV4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJV4"/>
    <dbReference type="GeneID" id="30027608"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="9"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="260" mass="29709" checksum="76B4E2242C953E4B" modified="2016-10-05" version="1" fragment="single">LLRLKSTDGFPSKYRNLLWLELSGASNKSVPGEFHRLLCLCQESSDPSIRTNVEQINLDVHRTLSSNKFFFDVEKCQPGPHFCKLQNILYAFIVHNPKVGYSQGMNRIVGNLLLATSEGSSQGTVGISEEGVFWMFVGIVEDLLPRYEQLFFFDPNALPFIQNDVSIAVKQHFANLLPQLFGHLNLLRVEIEIIVLGWWLGLFSEILKSLDIWFHVIDGLMLAKNPNVKLCAYSIAIFKLCERELFDLKTTGEVYSYFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJW5</accession>
    <name>A0A1A0HJW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D123-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC123 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714658.1">
        <property type="nucleotide sequence ID" value="XM_018853743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJW5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJW5"/>
    <dbReference type="GeneID" id="30026719"/>
    <dbReference type="OrthoDB" id="360540at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905143">
        <property type="term" value="P:eukaryotic translation initiation factor 2 complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009772">
        <property type="entry name" value="CDC123"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323:SF6">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 123 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15323">
        <property type="entry name" value="D123 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07065">
        <property type="entry name" value="D123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="340" mass="39168" checksum="51FA0679CAA6C97C" modified="2016-10-05" version="1">MTKLAFTFNDLPVTCAQVNECSYSSWSPHFASHIPEARILSDIPDAFLEYLNSDSIRLPPARYDDYVTANSDNEYSDWEDDIQESGASTAQAENPCAGFADFDKKIQEVVGKWRHVMPKLNWSAPKDARWILINNSLQCTSNADIYLLLNASDHIAHDLDGHIYDDCEDKDGGPRIKPEIVLKKWSRDWNPALEFRIFVKNRRILGVSQRDMNHYQFLQELHPKLVDLLERFQTQVIRKSAFPLKDYIMDVYVPAPHEKISIVDINPFARKWDSLLFTWHELLEKDNDGVFELRLITETNLGAMARKEHSENQVPIEVVDALMNTEAMVELAKTWGIKDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK24</accession>
    <name>A0A1A0HK24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_21500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714639.1">
        <property type="nucleotide sequence ID" value="XM_018855082.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK24"/>
    <dbReference type="STRING" id="869754.A0A1A0HK24"/>
    <dbReference type="GeneID" id="30028058"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="4"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="180" mass="20806" checksum="3BFB39677C093E13" modified="2016-10-05" version="1" fragment="single">KIQALVAHLRELKEQSPGEQVVVFSQFSTFLDLIENELKGLGSDGDLDFVVHKFDGRLNMVDREKVLRKFTAQNKADGRISVLLLSLKTGGVGLNLTVAKRAFMMDPWWSPSIEDQAIDRVHRIGQDQNVKVVRFIVKNSIETKMLRIQERKRMMGEAVEVEEEERRKQRIEEIKLLFDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK54</accession>
    <name>A0A1A0HK54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37583</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714886.1">
        <property type="nucleotide sequence ID" value="XM_018856893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK54"/>
    <dbReference type="STRING" id="869754.A0A1A0HK54"/>
    <dbReference type="GeneID" id="30029869"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="368"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="447"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="48"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="545" mass="60803" checksum="5831DF8A601078D5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKB2</accession>
    <name>A0A1A0HKB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glutathione degradosome</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_86434</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714941.1">
        <property type="nucleotide sequence ID" value="XM_018859259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKB2"/>
    <dbReference type="STRING" id="869754.A0A1A0HKB2"/>
    <dbReference type="GeneID" id="30032234"/>
    <dbReference type="OrthoDB" id="7832001at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051458">
        <property type="entry name" value="Cyt/Met_Dipeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017149">
        <property type="entry name" value="GSH_degradosome_Dug2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270">
        <property type="entry name" value="BETA-ALA-HIS DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43270:SF8">
        <property type="entry name" value="DI- AND TRIPEPTIDASE DUG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037237">
        <property type="entry name" value="Peptidase_WD_repeats_DUG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="9">
        <location>
            <begin position="705"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="970" mass="108289" checksum="2F4ED9AE43A1995B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKE3</accession>
    <name>A0A1A0HKE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger C3HC4 RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_10625</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714971.1">
        <property type="nucleotide sequence ID" value="XM_018853813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKE3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKE3"/>
    <dbReference type="GeneID" id="30026789"/>
    <dbReference type="OrthoDB" id="4090114at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033768">
        <property type="term" value="C:SUMO-targeted ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038886">
        <property type="entry name" value="E3_SLX5/Rfp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28042:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE COMPLEX SLX5-SLX8 SUBUNIT SLX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger C3HC4 RING-type" evidence="5">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="85"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="352" mass="39586" checksum="61D8A09090583242" modified="2016-10-05" version="1">MSERAQTKSPTPQVVEVESEDDLGSPVFPSSNYDVLRQNNVIEVTSDASGDEDVEIVAENSTHSNNDDVEITGHTTADPPMYEIEFPGGPPSRPRSIEPTLSRPTVGARSLDTPFSRNVRRRPNPRQNRNLYQARFYPRVQNPLWLSYDDIPAFLAEHSPQTRLFFQGQGDGDISSAIMDRISREEENSLDRKMESENRHNRKVLEKKKGVACDELDGYTNDIAPDDNVMCELCGIVLGEGIPTDFEPDLKYDENLEVHASSSQVMAPWYCIRQCFSTDRELSKRVFAAKCGHVFCGRCIKNIGNRPPSKRGKNSNHMTTLNPQISAPRKCPAEECGVAFNKSKKTFLEIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKF9</accession>
    <name>A0A1A0HKF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tryptophan--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_35514</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714991.1">
        <property type="nucleotide sequence ID" value="XM_018856620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKF9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKF9"/>
    <dbReference type="GeneID" id="30029596"/>
    <dbReference type="OrthoDB" id="15808at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070183">
        <property type="term" value="P:mitochondrial tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000082">
        <property type="entry name" value="MSW1p Mitochondrial tryptophanyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000002">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050203">
        <property type="entry name" value="Trp-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43766:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="368" mass="40796" checksum="A0D88909F7DBF2A6" modified="2016-10-05" version="1">MSPQTVSYIRSLSSVATLDKLSAFPRGSRIFSLIQPTGAVHLGNYLGALRNWKQISTEAPEDTQCIFGTADLHSVTQLPSSAALHENRYGAVANILLAGIDPKRCILFHQSSVPEHAELYWIFTCLTSMGSLNRMTQWKLKLKLEDDADIYSERAMGATKAGLLLYPVLQAADIMIYNATHVPVGEDQSQHLELSRDIASTFNHKYGKFFTLPQTILTPTKKIGALRDPTKKMSKSDANQSSSVFINDDADTIAKKFRRAVTDSVQGPVTFDPRNRPGVSNLVNIISGLLNKTVDETVKELAWVGSHKQLKDYVTELVVEEFKETKTMYDNLMADKSYIDAVCAEGTTKAREIALVNIKEIKRLVGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GQP7</accession>
    <name>A0A1A0GQP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_229930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000039">
        <property type="protein sequence ID" value="OBA14051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709218.1">
        <property type="nucleotide sequence ID" value="XM_018855237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GQP7"/>
    <dbReference type="GeneID" id="30028213"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA14051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="180" mass="20683" checksum="30CBD0C3A5948D42" modified="2016-10-05" version="1">MHDRPPFRNDETSEFRIQDTRPTPKFRGALDVNSGMALDAVPGYARPPRVRGQTLLITNTFNIRWLDKWNDKLLVAGGISPPYGFKISRCNTQNTCAANISENLYKYSRFEYERSSKICQLASRLNPELCNDFDILLTLTHWKNYSSFDLCTRNCKTLPSYYAVSAEEPVLAVRMYAPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYY2</accession>
    <name>A0A1A0GYY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">DNA replication regulator SLD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_48091</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Has a role in the initiation of DNA replication. Required at S-phase checkpoint.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709260.1">
        <property type="nucleotide sequence ID" value="XM_018858258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYY2"/>
    <dbReference type="STRING" id="869754.A0A1A0GYY2"/>
    <dbReference type="GeneID" id="30031234"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902977">
        <property type="term" value="P:mitotic DNA replication preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22289">
        <property type="entry name" value="RecQL4_SLD2_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1460:FF:000001">
        <property type="entry name" value="DNA replication regulator Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021110">
        <property type="entry name" value="DNA_rep_checkpnt_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040203">
        <property type="entry name" value="Sld2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28124:SF1">
        <property type="entry name" value="DNA REPLICATION REGULATOR SLD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11719">
        <property type="entry name" value="Drc1-Sld2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="47"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="275"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="359"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="210"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="359"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA16961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="408" mass="46285" checksum="0951F5258176DBC4" modified="2016-10-05" version="1">MSGLLEHKLRIKEWERGFAAKHGRVPSKADVKADVEIWKAYKAYNEMKKASRPAKREPDHPSKNPEGQRLKEQDSGSQNTENGFERVMPVQNAELGPTPQANGKVLSIFDLILSPPESSLVKQVQGKPGLFLPLSSLGPTSPFKTPTKSARPIHFGDVTPSRGPSVAEKLRMVSSPAKLAQTPTLLARHDVIETPRYLGKVNSKFSFASQHSPFQASPSKQHIFQTPTKPTPIENFQVSPSPLKSQRLLLSRLSDIYNAHQKLAMDEEYAAQRKEFEKEFEVDQPEPQGQAPSSENIKDMRKRKKATTQKRTTRRWKIKPRTEDGQNEAFDGKDVHEEIKKLETQERLELAQYIDGEVSAEEASDEEEEVVVRKKPASTKINPVSNNFQRLKINDPRAKKFKQRMRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZA3</accession>
    <name>A0A1A0GZA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Septin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_25806</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709324.1">
        <property type="nucleotide sequence ID" value="XM_018855302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZA3"/>
    <dbReference type="STRING" id="869754.A0A1A0GZA3"/>
    <dbReference type="GeneID" id="30028278"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032175">
        <property type="term" value="C:mating projection septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032152">
        <property type="term" value="C:meiotic septin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032169">
        <property type="term" value="C:prospore septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070583">
        <property type="term" value="P:spore membrane bending pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="18"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA17027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="44971" checksum="48255400FFA133A1" modified="2016-10-05" version="1" fragment="single">KVGLLNLPVQKEMWCKRKGAQFTMILAGCRGTGKTTFLNTLVGEELESEITTCEPMQVRDRKYHLQEDKFDMDLTVVDMPDFGANIDNQNTWLPLVKYIEYQYRSYLIQEEQPLRHQLKDNRVHVCVYFINPTNTELSSLDIESMKEISKRVSLIPVIARSDTLNKDELHNFKNIITKTLKQNEIEVCKYVTDTFATEKINTHSPYAIIGSNVLYTNPDGKLVRARKYGWGMVEVENSDHCDFVHIRDLLMSEHLLDLISAMETHYILFRQRFLRQRLNSAKTALNQTDPFPLNALNGEEDGLASYVMYKKTLGLDDMVMLEKYSGEEEQLQQEARNHLDDIIRKQEGKFREHKAALYTKQKIYNNDLEEIYQTVRLLEKEIEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Y2</accession>
    <name>A0A1A0H1Y2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ADP-ribosylation factor GTPase-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33784</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709434.1">
        <property type="nucleotide sequence ID" value="XM_018856398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Y2"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Y2"/>
    <dbReference type="GeneID" id="30029374"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF160">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN EFFECTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="546"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="692"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="921" mass="104380" checksum="2191D7EA10B12510" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H244</accession>
    <name>A0A1A0H244_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Palmitoyltransferase</fullName>
            <ecNumber evidence="13">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_33774</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709420.1">
        <property type="nucleotide sequence ID" value="XM_018856396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H244"/>
    <dbReference type="STRING" id="869754.A0A1A0H244"/>
    <dbReference type="GeneID" id="30029372"/>
    <dbReference type="OrthoDB" id="6781668at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161">
        <property type="entry name" value="ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24161:SF85">
        <property type="entry name" value="PALMITOYLTRANSFERASE HIP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 12" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0564">Palmitate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="497"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="623"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="679"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="325"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="355"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="15">
        <location>
            <begin position="575"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA18025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="858" mass="95445" checksum="FA6B043C1F0A0169" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H257</accession>
    <name>A0A1A0H257_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_47848</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709430.1">
        <property type="nucleotide sequence ID" value="XM_018858231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H257"/>
    <dbReference type="STRING" id="869754.A0A1A0H257"/>
    <dbReference type="GeneID" id="30031207"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039123">
        <property type="entry name" value="PPTC7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12320:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE PTC7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13672">
        <property type="entry name" value="PP2C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="3">
        <location>
            <begin position="136"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA18035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="401" mass="43277" checksum="185EA5FED285C9B2" modified="2016-10-05" version="1">MAAKNGGLRPLSATATNTLGASKHTPAAKPAGAPEPQTAANGGLLRTLKSVFGLAKLPEQQRLEAREQRENANSPFIQYPLVTREQMRRMASHAYRLDVGHASFAHHSFSADPTVHSLSDLTDPAQLNSLLPRRRPQGSPVDTLLIKAGDDAMLVLPTVMAIADGVSGWESKGEQASLGIWARSMVETLSRLLTEYRLSHMPHVLNARDVQQVLDDSFLHTSHLMDLQGLKGSSTLLLGMLSGATLKYISIGDSRLWVVRGGRVIRSNTEQMAAPLCPQQIGTQTLGRMPSEMAEVDEVALQENDLIVMCSDGISDNLFDHELVDALHQYLAPPNSSVRVACSKILAKCKLVAYDDNAYTPYNEKVNAPPGQAAKKASLGGKMDDMSICIARVVANESAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H280</accession>
    <name>A0A1A0H280_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Midasin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">MIDAS-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_19339</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the midasin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709427.1">
        <property type="nucleotide sequence ID" value="XM_018854857.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H280"/>
    <dbReference type="GeneID" id="30027833"/>
    <dbReference type="OrthoDB" id="5186at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000142">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000582">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000712">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001053">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001368">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048617">
        <property type="entry name" value="MDN1_AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041190">
        <property type="entry name" value="Midasin_AAA_lid_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17865">
        <property type="entry name" value="AAA_lid_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21108">
        <property type="entry name" value="MDN1_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="314"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="648"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="1084"/>
            <end position="1229"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="1370"/>
            <end position="1588"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="1761"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="2076"/>
            <end position="2314"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="3708"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA18032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="3708" mass="417969" checksum="56870853BEA8BB6F" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X8</accession>
    <name>A0A1A0H4X8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="4">1.1.1.302</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_47286</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes an early step in riboflavin biosynthesis, the NADPH-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HTP reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709626.1">
        <property type="nucleotide sequence ID" value="XM_018858143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X8"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X8"/>
    <dbReference type="GeneID" id="30031119"/>
    <dbReference type="OrthoDB" id="5432at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050765">
        <property type="entry name" value="Riboflavin_Biosynth_HTPR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011:SF7">
        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="Bacterial bifunctional deaminase-reductase C-terminal" evidence="13">
        <location>
            <begin position="26"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="245" mass="26944" checksum="D78A65C50D73E9E5" modified="2016-10-05" version="1">MSLLPLNASLVPFLKPYLPVPKDDRPFVTLTWAQSLDSRIASRPGVQTKISHLETKTMTHYLRACHDAILVGVGTVLADDPKLNCRYGNSYKIRPVVVDPSGKWDYTSSTLCKLRAEGQGLAPLILVSNDVNISTETENILNSQDGAFCRLPFDRMNRANNWELVLNALHNMGIKSVMVEGGAVVINDLIQSSMVDSVVITVGPVFLGRNGVEVSPAIERRLVDVDWWKGQRDSVVAGRLLPHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Y3</accession>
    <name>A0A1A0H4Y3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SANT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_89153</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709631.1">
        <property type="nucleotide sequence ID" value="XM_018859296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Y3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Y3"/>
    <dbReference type="GeneID" id="30032271"/>
    <dbReference type="OrthoDB" id="10258692at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051571">
        <property type="entry name" value="N-CoR_corepressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992">
        <property type="entry name" value="NUCLEAR RECEPTOR CO-REPRESSOR RELATED NCOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13992:SF39">
        <property type="entry name" value="SMRTER, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="684"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="900"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="748"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="748"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="767"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="950" mass="107702" checksum="228B5FBE4D038CC0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Z3</accession>
    <name>A0A1A0H4Z3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ras-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709641.1">
        <property type="nucleotide sequence ID" value="XM_018856385.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H4Z3"/>
    <dbReference type="GeneID" id="30029361"/>
    <dbReference type="OrthoDB" id="28245at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039360">
        <property type="entry name" value="Ras_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194:SF60">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN RASKOL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10194">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00323">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="1217"/>
            <end position="1401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2564" mass="292652" checksum="DD3AF204EA1F81FB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H516</accession>
    <name>A0A1A0H516_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF654-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709537.1">
        <property type="nucleotide sequence ID" value="XM_018856358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H516"/>
    <dbReference type="STRING" id="869754.A0A1A0H516"/>
    <dbReference type="GeneID" id="30029334"/>
    <dbReference type="OrthoDB" id="205993at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006994">
        <property type="entry name" value="TCF25/Rqc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684">
        <property type="entry name" value="NULP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22684:SF0">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT TCF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04910">
        <property type="entry name" value="Tcf25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="686" mass="78357" checksum="5C8D810C0FE708AD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H588</accession>
    <name>A0A1A0H588_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_18573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709650.1">
        <property type="nucleotide sequence ID" value="XM_018854794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H588"/>
    <dbReference type="SMR" id="A0A1A0H588"/>
    <dbReference type="STRING" id="869754.A0A1A0H588"/>
    <dbReference type="GeneID" id="30027770"/>
    <dbReference type="OrthoDB" id="4091477at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12331" checksum="C1D45F729CEC07FB" modified="2016-10-05" version="1" fragment="single">CNICGKLIVRDFSRHIRIHDETGRFQCIFPSGYCKHKSRKFNRPYDYKKHLLNIHFTFDDPAAKAAPNLTEKLHFRGQCNACGERFMANEWLETHILTTDLAMKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H592</accession>
    <name>A0A1A0H592_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribosomal protein L19/L19e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33686</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709612.1">
        <property type="nucleotide sequence ID" value="XM_018856379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H592"/>
    <dbReference type="STRING" id="869754.A0A1A0H592"/>
    <dbReference type="GeneID" id="30029355"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="113" mass="13307" checksum="AAEF91E452F7E13B" modified="2016-10-05" version="1">MGYGKRKGTKDARMPSQVVWMRRLRVLRRLLAKYRDAGKIDKSLYHSLYLSAKGNTFKHKRALVEHIIQAKAEAAREKSLKEEAEARRSKNRAARERRQQRVEEKRQAFLNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5A1</accession>
    <name>A0A1A0H5A1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL3m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_46942</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709791.1">
        <property type="nucleotide sequence ID" value="XM_018858105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5A1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5A1"/>
    <dbReference type="GeneID" id="30031081"/>
    <dbReference type="OrthoDB" id="274683at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000004">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_uL3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="29604" checksum="92C96D1513DE9908" modified="2016-10-05" version="1">MATLWTKAGLKLASSGVAPLQFQRGAARLTSIHAIPLTYTVAPVVNHSLTKSNERKTLLARPGLLGIKREMVSWFSPSGAQFAATIIEIDACEVVDTILFTEHGYSAVKVGHVDKLKNVDAGVLRMCEAAGVSPKAKISEFRVRTDEGLIQPGAELKADYFAVGQLVDVKGVTKGKGFAGVMKRHGFKGLGATHGVSVSHRSAGSMGPTQDPGRVLPGKKMAGRMGGNNCTVHNNEVLHADGDAGILVVKGQIPGPRGAYVKITDARKVYGKSLLSEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5H9</accession>
    <name>A0A1A0H5H9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thioredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709828.1">
        <property type="nucleotide sequence ID" value="XM_018858069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5H9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5H9"/>
    <dbReference type="GeneID" id="30031045"/>
    <dbReference type="OrthoDB" id="10264505at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019153">
        <property type="term" value="F:protein-disulfide reductase (glutathione) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03002">
        <property type="entry name" value="PDI_a_MPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45815:SF3">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291868" evidence="1">
        <location>
            <begin position="19"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="10"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="298" mass="33868" checksum="8504242FDCB27CF9" modified="2016-10-05" version="1">MKFHIFVLVSAVLTTVSGLGFYAKDSNIYELTPLNFDKVVLGSNYTTIVEFYAPWCGYCKQIKPVYHKLAKYFQEDAKYAINVAAVNCDEEKNKPLCSKYRVQGFPTLMVFRPPKFQRGSPTSSKHIPETYNGQRELPAIVDYMTSRLKNYVKKFHNFTSKSFVDWLNVEDGTEKVVVLSKADGVTPLLKSLAIDFLDSVSVAIVSVKNPVLSPTIKYGDEDIEIPLQEDDTLPVLLVYKQEEKKFVRYKGKKLNKMAKIQKFVMDTAGVVPSEGSLSKKEVKLRKYRGVKESIKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5P6</accession>
    <name>A0A1A0H5P6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_13698</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709941.1">
        <property type="nucleotide sequence ID" value="XM_018854414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5P6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5P6"/>
    <dbReference type="GeneID" id="30027390"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="289" mass="32662" checksum="AE3ECF36023C5A32" modified="2016-10-05" version="1">MSAQFNTKTYQLNNSKTIPAVALGTWLATDNDCVEAVSYALKNGYRHIDTAAHYGCEKEVGQGIRESGIPREEIFLTTKVWNFDHKNVKGAFEDSLSRLGLDYVDMYLLHWPKSLDPATEFPYKDWDFVDTYKEMQKLLDTGKVKSIGISNFTVAKARKLLADPGVTVKPVVNQIEGHPLLPQPELTKWFRDQNIVIECYCPLGSSETTLVKTDEIVDIAKRNNVDVGQVLISWGVQRNTVVLPKSTKKDRIISNLKTFTLSDEDMHILDTLTDKYGVQRAVPDDVFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H610</accession>
    <name>A0A1A0H610_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_222012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709885.1">
        <property type="nucleotide sequence ID" value="XM_018855148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H610"/>
    <dbReference type="GeneID" id="30028124"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="284" mass="31712" checksum="FEE1BD979843207E" modified="2016-10-05" version="1">MCIGGHTRIHIKKPHCTVAGPTLGPTQISHLLWHCGSMLLFCGYTRRLWLRQIPSPKLKTRRGRFHVVPEHLFLALGPGQNEASPSPGFGALAHPSNARDWPGKRDVRAGLGEKTLEKPQARPSTQVSFFCPPSFFRPPGSHPCLLSASPRYSPFSCPLHQKRNKKTHSFRPPFFSHCRSIFNRNTCYSVESERDTHSESLHPAEVAPLSEPRPNPIPRFLHHTFGTDAVEKLPAVTPKTLFSGFATPDCTSRLSRACFLGNCQRIVSARCSIVQKPDPPLLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6X4</accession>
    <name>A0A1A0H6X4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_206527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710230.1">
        <property type="nucleotide sequence ID" value="XM_018854996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6X4"/>
    <dbReference type="GeneID" id="30027972"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="117" mass="13516" checksum="ACC9C4FF649157F6" modified="2016-10-05" version="1">MLIGVDLEKERRSKAEYQLACMSGIFCHHCHKVGHMKVRCVQLRNKNPVSEKTTEPFFTHSMFIYGSNTHVVNDMSFFQTPKEELIFFETVITNLSTSFNIICMSNRGSGTCNSRWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H735</accession>
    <name>A0A1A0H735_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bul1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_44923</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710242.1">
        <property type="nucleotide sequence ID" value="XM_018857852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H735"/>
    <dbReference type="STRING" id="869754.A0A1A0H735"/>
    <dbReference type="GeneID" id="30030828"/>
    <dbReference type="OrthoDB" id="4007955at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039634">
        <property type="entry name" value="Bul1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022794">
        <property type="entry name" value="Bul1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007519">
        <property type="entry name" value="Bul1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31904:SF1">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04426">
        <property type="entry name" value="Bul1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04425">
        <property type="entry name" value="Bul1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bul1 N-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Bul1 C-terminal" evidence="3">
        <location>
            <begin position="500"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="733" mass="82802" checksum="928762DC66A32DF9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H749</accession>
    <name>A0A1A0H749_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_206796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710252.1">
        <property type="nucleotide sequence ID" value="XM_018855001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H749"/>
    <dbReference type="GeneID" id="30027977"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="158" mass="18431" checksum="FB8ED22D2B37ABF9" modified="2016-10-05" version="1">MGLYRRTVLSETVCVAARKPSASSPRQQSTNKRRTQETKKRKKKKNKQRKKQTSQRNKPEDERTQMNQLNKERTRTIKARPLKARPLKARTLKTPTLKTRPLKARTLKARTLKARTLKARTLKARTIKSTNIKNTTIKNTTIKNTNIKKTPTLKKHQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H755</accession>
    <name>A0A1A0H755_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_208027</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710330.1">
        <property type="nucleotide sequence ID" value="XM_018855010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H755"/>
    <dbReference type="STRING" id="869754.A0A1A0H755"/>
    <dbReference type="GeneID" id="30027986"/>
    <dbReference type="OrthoDB" id="268576at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000128">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_uL2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 11" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="10">
        <location>
            <begin position="104"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="9">
        <location>
            <begin position="211"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="362" mass="40469" checksum="AFE4A7731E273CB3" modified="2016-10-05" version="1">MFALNKRSWGSAAQTLLRFRSSVASSPSLHAAPGENLSDMTDLEMKDETHKRQRKLAATQFKMKIYANGENQPGSLNFRKPIHDHLHKGSPIKELTVVKKQTAGRNNTGKITVRGRGGGHKRRVRLVDSHRLVAGKQTVLRIEYDPNRSGHIALIKHNETGSLSYILASSGLRAGDMVESFRKGIPSDFLQEMKLTNNGEVDEALLNSRVMQKGNCMPLKMFPVGSIIHNIGLRQDGRGQLVKAAGTFGRLLSKAPEKHRVIVRLSSGEHRYIHEDCHATLGMVSNREHLVISWGKAGRSRHRGFRPKVRGVAMNAHDHPHGGGRGKSKSNKVSQSMWGIKKFARTRKFKRVNKMKVKDRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H0</accession>
    <name>A0A1A0H7H0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_32898</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710373.1">
        <property type="nucleotide sequence ID" value="XM_018856154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7H0"/>
    <dbReference type="GeneID" id="30029130"/>
    <dbReference type="OrthoDB" id="545683at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04938">
        <property type="entry name" value="TGS_Obg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.470:FF:000001">
        <property type="entry name" value="GTP-binding protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000030">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="5"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="411" mass="44984" checksum="57C8FAE96C8DF328" modified="2016-10-05" version="1">MGRETVIGLVGKPSSGKSTTLNALTDANAKVGSFPFTTIDPNKATGYMEMDCACKRFGKELLCKPNYGYCRNGTRGVPVMLLDVAGLVPNAHLGRGLGNKFLSDLTEADCLIHIVDVSGTTDAEGKETRGYDPLQDIEWLQDEIFRWILGNLTEKWGSVVRRHTSTKSSTLETLRAQLGGYYANKQLIGKALDQIPNLPPLQEWDSEMVETVVKAFMSVKFPTILALNKMDHPDSDKNVSKIMLKHPDTKCVLVSSLTEVLLRKLAAQNFIKYELGTEFVDTAEDLPDAGLRELDEKLANRIENIKDLVLYRFGSTGVSQLLQTAAEVLNLVPVYPVRNINTFGGSTGPNVFRDCVLVKRGTPVIQVARKIMGDVTIAAIEGVGGTRVGEEDTVEIGKNDILSFRLVPGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7L8</accession>
    <name>A0A1A0H7L8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79224</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710412.1">
        <property type="nucleotide sequence ID" value="XM_018859021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7L8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7L8"/>
    <dbReference type="GeneID" id="30031997"/>
    <dbReference type="OrthoDB" id="68611at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018820">
        <property type="entry name" value="BRE4-related_DUF2421"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052430">
        <property type="entry name" value="IVT-Associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049453">
        <property type="entry name" value="Memb_transporter_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47804:SF1">
        <property type="entry name" value="DUF2421 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10334">
        <property type="entry name" value="BRE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13515">
        <property type="entry name" value="FUSC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="637"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="690"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="714"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="739"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="779"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="927"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="Integral membrane bound transporter" evidence="7">
        <location>
            <begin position="664"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2421" evidence="6">
        <location>
            <begin position="846"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1020" mass="115302" checksum="BFFBBECA92E24D0D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7M7</accession>
    <name>A0A1A0H7M7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prefoldin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_45107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710510.1">
        <property type="nucleotide sequence ID" value="XM_018857879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7M7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7M7"/>
    <dbReference type="GeneID" id="30030855"/>
    <dbReference type="OrthoDB" id="29646at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027235">
        <property type="entry name" value="PFD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13303">
        <property type="entry name" value="PREFOLDIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="5"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="122" mass="14323" checksum="EB10955CE3C01DC7" modified="2016-10-05" version="1">MSEKQQQQEQKVKEHQLQYNRFQELLTDLQGQLSSIASQIQEHSIVDKTLTEIPPNKREGRKCYKMVGGVLVNKSVDEVIKILDEESKELTKSKELLEKEFTSCKKEMNDWMTKNKVKIVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Q4</accession>
    <name>A0A1A0H7Q4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1774-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710654.1">
        <property type="nucleotide sequence ID" value="XM_018859061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Q4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Q4"/>
    <dbReference type="GeneID" id="30032037"/>
    <dbReference type="OrthoDB" id="3342455at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013920">
        <property type="entry name" value="DUF1774_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37992:SF1">
        <property type="entry name" value="DUF1774-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37992">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08611">
        <property type="entry name" value="DUF1774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="260" mass="29531" checksum="37DA745F24302E71" modified="2016-10-05" version="1">MFDDLLTHKISTVVSWVLSTYSTVRYFSGRQPFSDDTPYHVGETPFTLNIIVTMAYWGLLIFMQILFVTQIFIPVVDSAAGVTNRLDYTKKVGWHFTAFNFFVFLWTMLFVILVLNLVNIFALYTTHKTYAIKPLGNYFLIHMTNAALPMSWLLFAIFWNGAVLFHVHKFLGRVIANFTVWLLLIIPGFFTLVFNDWGLGLTSSILAFGLGLGQLFTKAFALQWIFGFVISGLLLILTLLVATGRVVRRTTDSEAAPLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7R7</accession>
    <name>A0A1A0H7R7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_32960</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710468.1">
        <property type="nucleotide sequence ID" value="XM_018856175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7R7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7R7"/>
    <dbReference type="GeneID" id="30029151"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF1">
        <property type="entry name" value="ALLANTOATE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="428"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="521" mass="58577" checksum="A95F972B74AF853C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U7</accession>
    <name>A0A1A0H7U7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_32979</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. Reversibly leaves the enzyme after glucose depletion, causing the catalytic subcomplex V1 to detach from the V0 section.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710498.1">
        <property type="nucleotide sequence ID" value="XM_018856179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7U7"/>
    <dbReference type="GeneID" id="30029155"/>
    <dbReference type="OrthoDB" id="6605928at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00053565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="318" mass="36538" checksum="1943989592B76CFD" modified="2016-10-05" version="1" fragment="single">MESLSQYLVVSFPQSANAFPFLERQLQLGKQPLSEFNLPDFHIRTLDSLVQISEELAKLDSSIASSVSKVADIIDNVEQKKLSLDSRVVNGKSVSSFVENFSWNTSKYRLDKSIKDLVDIISSDALSLDNDVRQTFQQYQTAKSNFLAADRKKNGDLSIKSLHEIVRPEQFVLDSDHLTTVLIAVPKNLVGDFQKSYESLTQFVIPRSAQVIAKDDEYELYTVALFKKYEQDFISASREHKWHPRTDFVYSEENMNQMRKEFDVTKATEMKTKNDLIRLAKTAYSDIFSGWFHIKAIRVYVESVLRYGLPPQFDSFVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H824</accession>
    <name>A0A1A0H824_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_45509</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710573.1">
        <property type="nucleotide sequence ID" value="XM_018857924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H824"/>
    <dbReference type="STRING" id="869754.A0A1A0H824"/>
    <dbReference type="GeneID" id="30030900"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000009">
        <property type="entry name" value="Threonine--tRNA ligase 2, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000006">
        <property type="entry name" value="Threonine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF46">
        <property type="entry name" value="THREONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGS" evidence="15">
        <location>
            <begin position="67"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="300"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="722" mass="83489" checksum="10F4A80EE18E403F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H885</accession>
    <name>A0A1A0H885_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_45324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710627.1">
        <property type="nucleotide sequence ID" value="XM_018857906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H885"/>
    <dbReference type="STRING" id="869754.A0A1A0H885"/>
    <dbReference type="GeneID" id="30030882"/>
    <dbReference type="OrthoDB" id="4083233at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="380" mass="44192" checksum="3005EF4E5CC59F11" modified="2016-10-05" version="1">MTGPFEPEMIANLPQMTIQQLSKLEDCISDEFALLAREYVQARKSRVEYMQKNIESQIRTFQQNDTSWSSFYNQQVKLIDTLTNLTENNVIGDNTSSCHQKIDDTVLEIETQLKQLTASIELPLDLDVVFKHELGDLPFAEYFKEIVDLHKAQLVALLHHIQYQSDLTESITEKNRVIFWKQYKADVLQYKEKLINQTCQELENLHKEYHGISSEANEANTNSHYYRSVVPVEMSKADARCNTQYLLSTKNLDKYHGFDNIYFRNHRIEITDTKKAVQRRFQEFLDQQNQYKIAQLDSVAVKLAGCMGLSKEEAENDLALMRRRDGSTIDEQIHLGEVMCDDDLDFSSFSQEETYDSDVEDYHSILDMNRPPIRIDATTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8G4</accession>
    <name>A0A1A0H8G4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Proline dehydrogenase</fullName>
            <ecNumber evidence="2 5">1.5.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78712</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts proline to delta-1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17388"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.5.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the proline oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.2" evidence="2 5"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710802.1">
        <property type="nucleotide sequence ID" value="XM_018858926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8G4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8G4"/>
    <dbReference type="GeneID" id="30031902"/>
    <dbReference type="OrthoDB" id="5464at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004657">
        <property type="term" value="F:proline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.220:FF:000017">
        <property type="entry name" value="Proline dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002872">
        <property type="entry name" value="Proline_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015659">
        <property type="entry name" value="Proline_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914:SF0">
        <property type="entry name" value="PROLINE DEHYDROGENASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13914">
        <property type="entry name" value="PROLINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01619">
        <property type="entry name" value="Pro_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="FAD-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proline dehydrogenase" evidence="6">
        <location>
            <begin position="184"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="51120" checksum="8D05B3DB4ECA12EB" modified="2016-10-05" version="1">MSSRLVGSARFAKSFSAQYTGTFTSPFSPGRCFHKSIKKTSVAFAAGHFDSAFLKDFSTGELFLFMVIGMATLNKPILNICIKAFPYVPLPIIKALVYKIYCGGETIEDVKQTGQRLATRGINNMMISLTIEACEGNDNIDPQFIVDETKRSVSEILVPHTVSIIENSGKDINSIPAGYVALKPTGFTKNAAAVLRNHDSPENKAAFEEIVAGASAVCKTIYEANQKLAQKYPARTAPFVVGVIDAEKYDLQPGVYELQRRLYQLYNKLDAPVSVVGTLQMYLSGSSDLLAEEEKLARENKYRLGLKLVRGAYIHSEQQRASVIHKTKEDTDINYNRGISYCIESILGSQGNSSTVGHLVVASHNADSLKLATEKTYRGENSFQNTNKANICLGQLLGMADSITHDLIKTHKVDNVIKYVAWGPPLETREYLLRRLEENGDAVKNDTGLPLIKAAARAIFQRASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8X8</accession>
    <name>A0A1A0H8X8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_26841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710977.1">
        <property type="nucleotide sequence ID" value="XM_018855328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8X8"/>
    <dbReference type="STRING" id="869754.A0A1A0H8X8"/>
    <dbReference type="GeneID" id="30028304"/>
    <dbReference type="OrthoDB" id="3546279at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053157">
        <property type="entry name" value="Sterol_Uptake_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47784:SF5">
        <property type="entry name" value="STEROL UPTAKE CONTROL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="1">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="43342" checksum="5DE67E69F6416D78" modified="2016-10-05" version="1" fragment="single">SRKGCLSCKKLKIKCDEAKPCCEYCSHTGRVCVYPDQRISYKASINSEIKDCTDKDPAAGHVLRPRPYNLAVTQLQMTRFELRLLHLFHDQCVPYVTFQANKHTHRIWRHVFPKHFASLDLVRQSTYAMACLYLWPMANLQTLLQADQQESPGQTGSLEDLLFTHVFEGVSVFEDSAHSIFRKTASYFISSLQSSQLFLAEKQHGLDYDDFAALYFSGYLIFAYVGVHPHCVLPLLEDGTECPLDYIGFFASLHRLFSTAPLKIRDRLVDTLVEYSIWTPTAASQKIGLVETLRFQLPEFHFANATFGEIGPQMCLENETLEKTLYLFESCYFSAIREGHPTPLFKFLVLLGEDFINLARLRNYFALRILFTFSCVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H993</accession>
    <name>A0A1A0H993_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_199399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710979.1">
        <property type="nucleotide sequence ID" value="XM_018854910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H993"/>
    <dbReference type="STRING" id="869754.A0A1A0H993"/>
    <dbReference type="GeneID" id="30027886"/>
    <dbReference type="OrthoDB" id="7457040at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886:SF23">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE ICT1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42886">
        <property type="entry name" value="RE40534P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="143"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="507" mass="57116" checksum="245BDF55E5504E08" modified="2016-10-05" version="1">MSLISHAIEAMGSQSGAPAPARLASSIGMWSLVKDWWGVPLALRKPTEKAVGTPQYTAARRQNMKIEYALFRAVLSPEIHIFPVEEDAAELVLPEGCETDAAAPKIGARFIDTPLPSAAVAGARFIHEFEVTNASNTSVPRRHIVLVHGYMAAMGYFLKNVEAIARSYPNLVVHVIDMPGFGNSARPEFPSALLRLPGGASRADEIAQIIGVENWFIDQLEEWRREKNLVHFGLVGHSMGAYICSCYLLKYNRPDARIVDRFILVSPMGTESSDISLINNRKLQFNHHDVASDPLQEIFASQDFEHEDNKEDDWFHLWERLGKPRFPRNAVLKALWNNNISPFQLLQTFGPFYSKLLSFWSFQRFQNLSANFVEPAADEKPGGAKPQNADLILKLHEYSFSIFNQYQASGELAITKLINHEIVPRIPLCDRGFVEYLNDAKIKTLWMYGDRDWMNSKGGEYCVEKLRGLSDPDADLVVLKNAGHHLYLDNPEEFNNAVIGFFEPRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9E4</accession>
    <name>A0A1A0H9E4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bud site selection protein RAX2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711163.1">
        <property type="nucleotide sequence ID" value="XM_018856098.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H9E4"/>
    <dbReference type="GeneID" id="30029074"/>
    <dbReference type="OrthoDB" id="2503993at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005621">
        <property type="term" value="C:cellular bud scar"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902929">
        <property type="term" value="C:plasma membrane of growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024982">
        <property type="entry name" value="Rax2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048266">
        <property type="entry name" value="Rax2-like_second"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048265">
        <property type="entry name" value="Rax2-like_third"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31778:SF2">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN RAX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12768">
        <property type="entry name" value="Rax2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20842">
        <property type="entry name" value="Rax2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20843">
        <property type="entry name" value="Rax2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291743" description="Bud site selection protein RAX2" evidence="2">
        <location>
            <begin position="19"/>
            <end position="1332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1268"/>
            <end position="1293"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like second" evidence="4">
        <location>
            <begin position="246"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like third" evidence="5">
        <location>
            <begin position="447"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="Rax2-like C-terminal" evidence="3">
        <location>
            <begin position="990"/>
            <end position="1254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1332" mass="144095" checksum="FC8D68F6E4DB981E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9F0</accession>
    <name>A0A1A0H9F0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DUF221-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_230239</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711142.1">
        <property type="nucleotide sequence ID" value="XM_018855244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9F0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9F0"/>
    <dbReference type="GeneID" id="30028220"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="488"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="583"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="622"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="681"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="730"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="758"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="27"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="214"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="9">
        <location>
            <begin position="488"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="329"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="873" mass="97266" checksum="6499A3B7F0B6E489" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9I2</accession>
    <name>A0A1A0H9I2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_83776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711306.1">
        <property type="nucleotide sequence ID" value="XM_018859234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9I2"/>
    <dbReference type="GeneID" id="30032209"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012881773" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="16386" checksum="3B5128CEC4BDA4C5" modified="2016-10-05" version="1">MKLLATFPLLALCFAVSLKGTFLIETGGEKVGAPKFLGVSKDGTVTQSFKGDVFELQSGESKLLKNIVLKDGKYIVIANGTLSMSKETFPVDDNTLSIVNATEDYVNVADEYLYYGFSVHFRLFDDSTIHEFLSEEGQKIKIKFVESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9X0</accession>
    <name>A0A1A0H9X0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rcd1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711333.1">
        <property type="nucleotide sequence ID" value="XM_018858996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9X0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9X0"/>
    <dbReference type="GeneID" id="30031972"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000160">
        <property type="entry name" value="Cell differentiation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="33364" checksum="34F31ACFC0B0FB75" modified="2016-10-05" version="1">MPLAPPQPPSRALADKQIFQWLSELVSGNNRERALLELGKKREQYDDLALVLWNSFGVMSVLLEEIIAVYPFLNPPVLTASVSNRVCNALALLQCVASNKQTRGLFLNANLPLYLYPFLLTNARQRSFEYLRLTSLGVIGALVKNDTPEVINFLLATEIVPLCLNIMEISLELSKTVAIFILQKILLDDQGLLYICTTYERFHTVALVLSKMIEQLGVAAQNAPRQPADPAAGPPPSSSGRLLKHVVRCYMRLSDNLEARKALSTILPDPLRDGTFAKILQDDAATKRCLAQLLLNIRDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAN5</accession>
    <name>A0A1A0HAN5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711586.1">
        <property type="nucleotide sequence ID" value="XM_018854050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAN5"/>
    <dbReference type="GeneID" id="30027026"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="129" mass="14423" checksum="D05E09F9E6790347" modified="2016-10-05" version="1">MGKGGKGSSGGGHSAGYPSTTGNPSGGGRDNGPRSGNLLQLPAWFFNWPQERVRVWNYVEIIPWFGPFQCRDTLQNYDRLFEQTPNNLAQRKQMFVVQRELFGASLDGSLLNIQKWTHAGYLQVFESSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAR1</accession>
    <name>A0A1A0HAR1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histone acetyltransferase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_42200</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711719.1">
        <property type="nucleotide sequence ID" value="XM_018857512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAR1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAR1"/>
    <dbReference type="GeneID" id="30030488"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="281"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="338"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="390" mass="43972" checksum="478260C4B8F233D1" modified="2016-10-05" version="1">MDDSQNHLQLKEEYELWRKNCRFMYDFVSETALTWPSLTIQWLPQHEENNAKLLLGTHTSGMDDNYLKLASTKLPVKSNSDIDKTARTSSKIKISKKIKTSSEINRARYMPQDPNLVAAINGAGGCEIFNLAKGIKELTLASHSENGYGLSWNHFQRGILLTGSDDKTCVISDVNRSNSEQFLERYLSHTDIVNDARWHTFDTNVFGSVSDDRNVYLFDTRAHNKPVQMFHDEASEGINSLAFSPFSTNLLAVGNQNSNISFLDLRVLDLKSSKGDALHTMMGHGDGITSMEFSPHRDGILASGSQDRRVILWDLCRIGEEQTQEDAEDGAPEIFMMHAGHTSAVTDLSWCPFQDWTLASTADDNILHLWKVSDLLTKYETGEFEVPRLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAT1</accession>
    <name>A0A1A0HAT1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_175656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711631.1">
        <property type="nucleotide sequence ID" value="XM_018854721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAT1"/>
    <dbReference type="GeneID" id="30027697"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="293" mass="33425" checksum="7E5B8B863299DC2C" modified="2016-10-05" version="1">MLWLGMIHYMVYDARQARWAKEAKRALAASRYFGAWSGCSYLCFQGHRTLWCTGTSLRGHLVIPEGILYIVDEESRARYIILLLDEQAQEAAAPRNSIIRGIGKFPEAGVFFPEARGVLFRGPGCFWLTAMESMSRNVWPKTPSQPAAPNPHTARRYRGTEQTRHAASTRASTKRERSLGRRRTGVRFYVPDDAHYRKRPLMCADAEPCIHQSIILCFLSPKAGPRDRRSSRHEREPRPERCGRLEMRSPAASEEVWAARAGRLDTACHCRSRPTGTEPNEAETRPMRQRQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAT2</accession>
    <name>A0A1A0HAT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_42867</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711739.1">
        <property type="nucleotide sequence ID" value="XM_018857593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAT2"/>
    <dbReference type="STRING" id="869754.A0A1A0HAT2"/>
    <dbReference type="GeneID" id="30030569"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000011">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="8">
        <location>
            <begin position="45"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="38787" checksum="917AF2F22F50CC4E" modified="2016-10-05" version="1">MTSQYKQEKLDHTPWVEKYRPKNLDDVASQDHAVKVLKKTLQLANLPHMLFYGPPGTGKTSTVLALAKQLYGPRLYKLRVLELNASDERGISIVRLKIKNFARLSVSNPTTEDLANYPCPPYKIIVLDEADSMTNDAQAALRRTMENYSGVTRFCLICNYITRIIDPLALRCLKFRIRLLDSVNALARLSHVADLEHVRVALAEVYDELISVSNGDLRKAITYLQSAARLDSSLQSENFGLSNGITVDSVREIAGVVDQSLMSSLVTVIKLKDTAKLASAVHQVVLLGWSAQQVSDQLHDILILDDTLSSAQKNEVATALFDTDRRLNNGTDEHIQLLNLMLLISKAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAU0</accession>
    <name>A0A1A0HAU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31734</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711641.1">
        <property type="nucleotide sequence ID" value="XM_018855902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAU0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAU0"/>
    <dbReference type="GeneID" id="30028878"/>
    <dbReference type="OrthoDB" id="6247875at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01389">
        <property type="entry name" value="HMG-box_ROX1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000041">
        <property type="entry name" value="HMG box family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF161">
        <property type="entry name" value="SEX-DETERMINING REGION Y PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="154"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="154"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="346"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="483" mass="55088" checksum="6B16B1A75CE60041" modified="2016-10-05" version="1">MSASTFFPQEVPVESCKLSSKPYSLQLSAQQQQPANRHLPAILPIMNMGQLPQIKAEEASQALNYFNNPYPLYCQASNKGEGDLSELLQSSSNSSSSANQPSMNYYSATVSPQTPYSNALVASAPGPLQQTPRKERKYQKYSERCTCKRDENHIPRPRNAFILFRQKHHQSVLKESTKLKTNPDVSRELARRWKALTKDERSHWDSLADEEKRNHAIKYPNYRYKPRRHKNSKLCEICLVKPMSTNTPPVQTFAMTQDFSQVSSNQASKLVRLPAIPLNHYQLHNQEQQPTQLFHQQMNGQFQLPQQYPQLIPQSEAMYISHRYQQYGNCPQQSQYEFGDEISSHNHAFQQQHQQSASTQPQTQHQMQQPYMTQIQRRNSLTQHQILQHSPQHHPQADNYATIAPLQHLDQHPPQAPLGGTTHGVIGAQINGYPDKYCETSDSFSFPDQAGQGQKYTGLPNPIGAPMYAAHDPFTAPTLLQLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAZ7</accession>
    <name>A0A1A0HAZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endoplasmic reticulum lectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein OS-9 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_41098</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Lectin involved in the quality control of the secretory pathway. As a member of the endoplasmic reticulum-associated degradation lumenal (ERAD-L) surveillance system, targets misfolded endoplasmic reticulum lumenal glycoproteins for degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
            <orientation evidence="1 7">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the OS-9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711809.1">
        <property type="nucleotide sequence ID" value="XM_018857354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAZ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HAZ7"/>
    <dbReference type="GeneID" id="30030330"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045149">
        <property type="entry name" value="OS-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012913">
        <property type="entry name" value="OS9-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414">
        <property type="entry name" value="OS-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07915">
        <property type="entry name" value="PRKCSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="MRH" evidence="8">
        <location>
            <begin position="42"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="417" mass="46913" checksum="775D826ACE7B03E6" modified="2016-10-05" version="1" fragment="single">MHPKEPPRPHPLLEAREEIAAETPAAILGNAMALIHAALPSGVCLWAYDLRGTYWTYALCSGDKIIQYHEGAPPHERGLRHVPLHPSTVFVLGRFSDASTAEVRFHNQAPAAEFRAYEKAAGRAAVLVDEKAAPYSHQRVQKAVLQTVTGGSVCDMTLQPRTAEVVYRCAENGGPDAAILDVFEIKTCHYKMLVHVPGLCEYAPFSPHKNTLDAALDVPCQRVRDPREAQVQRYATFDEYRYNIELREHVPFPVRADNRLDIADHFLAPVGRGFYFAHSRRELGTPSSYFNERNVVLFSGWCLSLTDLNAQFAKVIFRAVGSSLLAPSPPELDAKTLEWLHSFVLWYEIYDYKGDLLAVTRLEHDASEDTATLKAQLLDPVTLLDVEGDMPRMSSFERPDFEAPYDAWNFELFSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB25</accession>
    <name>A0A1A0HB25_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_42512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711592.1">
        <property type="nucleotide sequence ID" value="XM_018857547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB25"/>
    <dbReference type="STRING" id="869754.A0A1A0HB25"/>
    <dbReference type="GeneID" id="30030523"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="830" mass="91289" checksum="68FABADB01A76DD1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBB7</accession>
    <name>A0A1A0HBB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Required for respiratory growth protein 7, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_179699</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711816.1">
        <property type="nucleotide sequence ID" value="XM_018854758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBB7"/>
    <dbReference type="GeneID" id="30027734"/>
    <dbReference type="OrthoDB" id="20734at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018828">
        <property type="entry name" value="RRG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133:SF1">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10356">
        <property type="entry name" value="RRG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="238" mass="26439" checksum="3A3FE9FC797647AE" modified="2016-10-05" version="1">MLRFLPRRGLATLKEVQSCQEYLDFCRLKGTPMNSTVFRGTLYELTAKALLEQSLHFSDLVRSGGAGDNGLDLYGRWDLSHYRPADGAIRKPPASSLLLACKPFSAQNNALDLRQDVVVLVQCKNHRVKIKAATIRELAGIREFHINMTSKTERRKTFMVLVSPLPMTKQGTQQMDTSDVPMLHAQVSPLEAPLVPGPGDFCLQKWKGGVLGPVYLNFSARTLLQGLSVECKLGEVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBE7</accession>
    <name>A0A1A0HBE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711969.1">
        <property type="nucleotide sequence ID" value="XM_018855956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBE7"/>
    <dbReference type="GeneID" id="30028932"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291734" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8325" checksum="BDACB384064D9BDE" modified="2016-10-05" version="1">MVILLGLVRESILQGLLALLLPEDAVCKCWQKRATGKCSNRRGSVISIFRLRGFNLQDASTASEGPQNSPHMEWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBM9</accession>
    <name>A0A1A0HBM9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_179513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711792.1">
        <property type="nucleotide sequence ID" value="XM_018854755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBM9"/>
    <dbReference type="GeneID" id="30027731"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="13424" checksum="F85C886D71D496AA" modified="2016-10-05" version="1">MPCMSFLLTLRIGCLASSSHIYWLHYILGVLYSLSHWVYYIRCLIGFTTFAVSLGLLHSLSGCTTSTVSYWSSHIGYLTLENHGLLHVLAIGVFPASAALSCSDSKAHATTTPVCFSNKSESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC56</accession>
    <name>A0A1A0HC56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_14323</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711983.1">
        <property type="nucleotide sequence ID" value="XM_018854493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC56"/>
    <dbReference type="STRING" id="869754.A0A1A0HC56"/>
    <dbReference type="GeneID" id="30027469"/>
    <dbReference type="OrthoDB" id="552049at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901981">
        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026894">
        <property type="entry name" value="DnaJ_X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052814">
        <property type="entry name" value="Peroxisomal_DnaJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45006:SF1">
        <property type="entry name" value="DNAJ-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14308">
        <property type="entry name" value="DnaJ-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="396" mass="42974" checksum="748B1FD745002602" modified="2016-10-05" version="1" fragment="single">MVKDTTYYDIVGAAPGATEVELKKAYRKRAIQLHPDKNGNDPGAAAKFQELGQAYAVLSNAESRALYDEVGVEGLSESRAETAADIDPSELFKMIFGGGSFAPWIGQLSMFDEMSETAEILAESDDEAGGLQAQGGQASTAQASEPGSPGPAEQPPAGASNAPSENGNTDTSTNGNTETSKKHKKKMTRAQRERLVAAHDASRRRKEERIQALTAALVSRIDSLRSCASAEARDGFRGKLRRELEDLKIESFGIQLLHLIGRIYVQQAHATVQASKTFGVSKLFTSVKSKSERVKSGFQIVKSALDAQASAEDMLRHQAELELGGAELTEHDRHQQMEAERLITGKFLATAWASTQFEVQGVLNKVAQRVLNDKALPKKERVARARAMLFIGNEML</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG8</accession>
    <name>A0A1A0HCG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_229862</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712092.1">
        <property type="nucleotide sequence ID" value="XM_018855234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG8"/>
    <dbReference type="STRING" id="869754.A0A1A0HCG8"/>
    <dbReference type="GeneID" id="30028210"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="73"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="209" mass="22720" checksum="18F81C22DC8BA4EB" modified="2016-10-05" version="1">MSQPESDFKAQFGSWASRTASQSGASSAPAFSDWSDYVRSGANNLYDSLPSYNTSGAAAQEPSWFQLSRFERVVAFGCCLGASLLCFTLGFFVFPVLALRPTKFAVLWLMGLLLFVVSFGVLQGPNAYIRHITSSGRILFSAVFFGLILTTMYCAVVLKSTVLTIVASVVEVFAILYYAFSYFPFGTATITWFSSYVVGYVGGLFAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCN7</accession>
    <name>A0A1A0HCN7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial</fullName>
            <ecNumber evidence="6">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="6">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_42653</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterodimer of an alpha and a beta subunit. Different beta subunits determine nucleotide specificity. Together with the ATP-specific beta subunit SUCLA2, forms an ADP-forming succinyl-CoA synthetase (A-SCS). Together with the GTP-specific beta subunit SUCLG2 forms a GDP-forming succinyl-CoA synthetase (G-SCS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712167.1">
        <property type="nucleotide sequence ID" value="XM_018857567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCN7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCN7"/>
    <dbReference type="GeneID" id="30030543"/>
    <dbReference type="OrthoDB" id="1664372at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000005">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="27"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="6 7">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="119"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="324" mass="33965" checksum="9683009EF2F1044C" modified="2016-10-05" version="1">MLSKQFARGFASTPLAKNYASTIKNLKVTKDTKVIYQGFTGKQATFHAEQAIAYGTQVVGGTNPKKAGTQHLDRPVFASVKDAMRETGASATGIFVPPSIAAKAIEEAIEAEIALAVAITEGIPQLDMVRIAQILKTQSKTRLVGPNCPGLIAPDQCKIGIMPSHIHQRGRVGIISRSGTLTYEAVAQTTQVGLGQSLVIGMGGDPFPGTNFIDALTLFLNDKETEGIILIGEIGGTAEEEAAEFLKQHNLTRPEGPKPVVSFIAGVSAPPGRRMGHAGAIVAGGKGDAKSKIASLESAGVVVEQSPARLGNSLLQEFKNKGLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCZ4</accession>
    <name>A0A1A0HCZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_10734</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712304.1">
        <property type="nucleotide sequence ID" value="XM_018853840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCZ4"/>
    <dbReference type="GeneID" id="30026816"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025795">
        <property type="entry name" value="tRNA_(uracil-5-)_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51622">
        <property type="entry name" value="SAM_MT_RNA_M5U_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="442" mass="49352" checksum="126CA0641E8897A8" modified="2016-10-05" version="1">MSSEGEGLGLVPRSAYDDQCTDPEVKTVIKLPKAVPGDVSTALLKRHHDYYAEAELVSVSRAPVRKGLRNDKLIICEHFGQCSGCQLQMLPYENQLEFKLDIIKRAYKYFYPKLDCSKVEGFGLVVDSPMQFSYRTKLTPHTVVPKKFGMEFLPVPVGFNNAVLGNPIVDINHCPIATSPINAVIPELKKQLNDKLAAKLESKSKIKVAPDFILRDSIRINHATGEYTNVCLTQRNNVVTEKVKEFVFQFEANEFFQNNRSILPTFIDFISFHLSHVNFKHIVDAYCGSGFLGISLSSQLPKDGKVFGIEISKKSIEYAKHNAGINGIPMPEKMMFVSGNSDSIFTDGNFLGSGVHGSNSVVIMNPSRKGSTDEFIQQLVDFRPKAIIYVSCNVFTQARDLASLQTFADRLGVNYQVKTITGFDFYPQTKHVESVAVLELQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCZ6</accession>
    <name>A0A1A0HCZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcriptional regulatory protein DEP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_10872</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712460.1">
        <property type="nucleotide sequence ID" value="XM_018853868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCZ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCZ6"/>
    <dbReference type="GeneID" id="30026844"/>
    <dbReference type="OrthoDB" id="20886at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="84"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="351"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="254"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="370"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="400"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="465"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="720" mass="80850" checksum="9191FAE6A9334D5E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCZ7</accession>
    <name>A0A1A0HCZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_38865</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712346.1">
        <property type="nucleotide sequence ID" value="XM_018857060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCZ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCZ7"/>
    <dbReference type="GeneID" id="30030036"/>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001002">
        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001003">
        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="865" mass="99907" checksum="5002827E58197CF5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD98</accession>
    <name>A0A1A0HD98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methionine aminopeptidase 2</fullName>
            <shortName evidence="9">MAP 2</shortName>
            <shortName evidence="9">MetAP 2</shortName>
            <ecNumber evidence="9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MAP2</name>
        <name evidence="12" type="ORF">METBIDRAFT_77425</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712368.1">
        <property type="nucleotide sequence ID" value="XM_018858699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD98"/>
    <dbReference type="STRING" id="869754.A0A1A0HD98"/>
    <dbReference type="GeneID" id="30031675"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="11">
        <location>
            <begin position="31"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="325" mass="36409" checksum="9A789A9AC328BBFB" modified="2016-10-05" version="1">MEYPLEVNSYRTTDEEKRYLDRQQNNHWQDFRKGAEIHRRVRAKAKQNIRPGMSMLEIADLIENSIRGYTGNDHTLTQGIGFPTGLSVNHVAAHYTPNSSDKMSLKQEDVMKIDIGVHVNGHIVDSAFTLTFDDKYDNLLTAVREATYTGVKEAGIDVRLNDIGAAVQEVMESYEMELGGKTYPIKCIRNLNGHNIGDYVIHSGKTVPIVANGDMTKMEEGETFAIETFGTTGKGYVVTQGECSHYALNQNIDGVKLPSQRAKELVKSIQSNFGTLPWCRRYLERAGEDKYLLALNQLVRAGVVEDYPPLVDTTGSYTAHFATQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDA9</accession>
    <name>A0A1A0HDA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Selenoprotein O</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_39257</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712378.1">
        <property type="nucleotide sequence ID" value="XM_018857119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDA9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDA9"/>
    <dbReference type="GeneID" id="30030095"/>
    <dbReference type="OrthoDB" id="10254721at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003846">
        <property type="entry name" value="SelO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32057:SF14">
        <property type="entry name" value="PROTEIN ADENYLYLTRANSFERASE SELO, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02696">
        <property type="entry name" value="SelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="615" mass="68585" checksum="3762B4229FBA00BF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDJ1</accession>
    <name>A0A1A0HDJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">V-type proton ATPase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_39923</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712458.1">
        <property type="nucleotide sequence ID" value="XM_018857204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDJ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDJ1"/>
    <dbReference type="GeneID" id="30030180"/>
    <dbReference type="OrthoDB" id="10261947at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10580:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005772">
        <property type="entry name" value="ATPase_V1-cplx_fsu_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01101">
        <property type="entry name" value="V_ATP_synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015945">
        <property type="entry name" value="ATPase_V1_F_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="124" mass="13723" checksum="8F717C38CD59D0E9" modified="2016-10-05" version="1">MSANEAMAKRSLLAVIADEDSVTGLLLAGVGQVSNEPGKEANFLTVVPGKTSEEEVEEAFDKFTSERDDIAILLINQHIADLIRHRVDSYTKAFPAILEIPSKDHPYDPEKDSILKKVRRLFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDL6</accession>
    <name>A0A1A0HDL6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_82055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712514.1">
        <property type="nucleotide sequence ID" value="XM_018859217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDL6"/>
    <dbReference type="GeneID" id="30032192"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012949646" evidence="1">
        <location>
            <begin position="16"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="126" mass="14529" checksum="A456B35527B03294" modified="2016-10-05" version="1">MRRFAIFSLLTLTLAATQQWREIPMIVQDVEGVSPPKYIFIDTDGLANLSDLEFLYVFKDGVMKDNHGYLGLDTEQQLCMGFGESVPFENGYFRYNDDPVFELCTDYIIRYNSGCQILKHIKITIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDX8</accession>
    <name>A0A1A0HDX8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_67615</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712681.1">
        <property type="nucleotide sequence ID" value="XM_018858366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDX8"/>
    <dbReference type="GeneID" id="30031342"/>
    <dbReference type="OrthoDB" id="4079278at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="621" mass="71858" checksum="8FB9CD7DAB9C4A86" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE26</accession>
    <name>A0A1A0HE26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Catalase</fullName>
            <ecNumber evidence="12">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_77780</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 13">Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 H2O2 = O2 + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the catalase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712850.1">
        <property type="nucleotide sequence ID" value="XM_018858763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE26"/>
    <dbReference type="STRING" id="869754.A0A1A0HE26"/>
    <dbReference type="GeneID" id="30031739"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08157">
        <property type="entry name" value="catalase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000001">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="7 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 12" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase core" evidence="14">
        <location>
            <begin position="6"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="482" mass="54530" checksum="FB749DAE569C5EC8" modified="2016-10-05" version="1">MAPVYTNTNGCPVPDPMASQRIGKHGPLLLQDFNFIDSIAHFDRERIPERVVHAKGSGAYGVFEVTDDISDICSSKFLDTVGKKTKVVTRFSTVGGESGSADTARDPRGFSVKFYTEEGNLDFVYNNTPVFFLRDPTKFPHFIHTQKRHPQTHLKDANMFWDYLCSNEESAHQVMILFSDRGTPRSYREMHGYSGHTYKWSTKNGDWHYVQVHLKSDQGFKTLTNEEAGALAGSNPDYAQEDLFKEIAKGNHPSWTCYIQTMTAEQAKHVDFSVFDLTKVWPQKDFPLRRFGKITLNENPKNYFAEVEQVAFSPSHSVPYMEPSADPVLQSRLFSYPDTHRHRLGTNYQQIPVNCPVTGAFHPHIRDGAMLVNGNMGSTPNYYSAANNFATERFSLQEDQEVWEGAACPFHWKATDAEFKQATDLWHVLARTPGQQENLAHNVVAHASAADVPIQDRVVAYFTKVHPDLGQAIKKGLEESRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE42</accession>
    <name>A0A1A0HE42_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712751.1">
        <property type="nucleotide sequence ID" value="XM_018857146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE42"/>
    <dbReference type="STRING" id="869754.A0A1A0HE42"/>
    <dbReference type="GeneID" id="30030122"/>
    <dbReference type="OrthoDB" id="10251809at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="990" mass="111590" checksum="63D8F54F4727C90D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEC8</accession>
    <name>A0A1A0HEC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ARF/SAR superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_77710</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712754.1">
        <property type="nucleotide sequence ID" value="XM_018858748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEC8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEC8"/>
    <dbReference type="GeneID" id="30031724"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034067">
        <property type="term" value="P:protein localization to Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04151">
        <property type="entry name" value="Arl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000510">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="185" mass="20681" checksum="C3F3C209B36D9EDB" modified="2016-10-05" version="1">MGQVLSFTSIFSKLWGVQKEIRILILGLDGAGKTTILYRLQMGEVVTTKPTIGFNVETLKYKNLTLNIWDLGGQTSIRPYWRCYYTNAAAVIFVVDSTDKDRIDVASKELHMMLKEEELLDSALLVFANKQDQPGALTAAEVSQTLNLTDLKDRSWSIVASSAIKGEGLTEGLDWLMDVIKDEQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEF8</accession>
    <name>A0A1A0HEF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_77735</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712784.1">
        <property type="nucleotide sequence ID" value="XM_018858751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEF8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEF8"/>
    <dbReference type="GeneID" id="30031727"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004500">
        <property type="entry name" value="Pro-tRNA-synth_IIa_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050062">
        <property type="entry name" value="Pro-tRNA_synthetase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00409">
        <property type="entry name" value="proS_fam_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42753:SF2">
        <property type="entry name" value="PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="58"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="554" mass="61516" checksum="0ED9DCE520049ABC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEG1</accession>
    <name>A0A1A0HEG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">E3 ubiquitin-protein ligase PEP5</fullName>
            <ecNumber evidence="9">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_39147</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex. Component of the class C core vacuole/endosome tethering (CORVET) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Vacuole membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the VPS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712877.1">
        <property type="nucleotide sequence ID" value="XM_018857102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEG1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEG1"/>
    <dbReference type="GeneID" id="30030078"/>
    <dbReference type="OrthoDB" id="26184at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057308">
        <property type="entry name" value="CHCR_PEP5_VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057307">
        <property type="entry name" value="PEP5_VPS11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016528">
        <property type="entry name" value="VPS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024763">
        <property type="entry name" value="VPS11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF24">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23341">
        <property type="entry name" value="PEP5_VPS11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23356">
        <property type="entry name" value="TPR_PEP5_VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12451">
        <property type="entry name" value="VPS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17122">
        <property type="entry name" value="zf-C3H2C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007860">
        <property type="entry name" value="VPS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0926">Vacuole</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="899"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="997" mass="111020" checksum="7E72BE03AE33E5F9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEK0</accession>
    <name>A0A1A0HEK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_11362</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713035.1">
        <property type="nucleotide sequence ID" value="XM_018853967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEK0"/>
    <dbReference type="SMR" id="A0A1A0HEK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEK0"/>
    <dbReference type="GeneID" id="30026943"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006694">
        <property type="term" value="P:steroid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="3">
        <location>
            <begin position="10"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="37270" checksum="FDDF92B80BD06258" modified="2016-10-05" version="1">MTVTPTTVFVSGATGFIAQHIVKLLIDKGYNVVGTVRSSEKGENLKKLLNSNSFSYEIVKDVEPAGAFDEALKNHPEVTVFLHTASPFHFKASDAEKELLKPAVNGTKNALNAIKTYGPQIKHVVVTSSYAAIMNLSKGNDPSHVDTEELWNPITWEEAKLGPGPGYVGSKTFAEKAAWDFVKDEKPNFTINYINPVYVFGPQTFDSEVKDELNTSSEIINGLLNLGKDSDVPETAGSFVDVRDVAKAHLVAFEKNLSNQRLLMTSSKFNGQDLLDVLNARFESLKNKLPIGNPGSGKEAAKQTCQLDNSKTREILGFPLIDLETSVVDTVAQILKARGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEP1</accession>
    <name>A0A1A0HEP1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="6">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_17602</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="6">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712853.1">
        <property type="nucleotide sequence ID" value="XM_018854724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEP1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEP1"/>
    <dbReference type="GeneID" id="30027700"/>
    <dbReference type="OrthoDB" id="193716at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="47"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="270"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="502" mass="57902" checksum="5BAC92EEE1EC5206" modified="2016-10-05" version="1" fragment="single">PNLYEVLGKEEITLAEFKEHPAFNSWLKKAVHSLNFQEFTHVQQKTILPFLQEEGLVCRAKTGTGKTYSFVIPLMQHVMDIFETNKMRRRNVSCLVIAPTRDLAQQIHADFRSLNMNNPHLRHKCDVRLWTGGLPPGKRPWGSDVPQIIVGTPGRILANLEDRRWAQHFNELEFRVYDEADRLLDQGFEDQLNAIDAKIKSLRSEKFEPPLRTLLFSATTNDRVTRFAEAQMGKDYKYINCVDDSEAESHAHIEQRLIKTPGIYESHVAAVDEILSNIESKDYKAIFFLPTVVGADFMFRLLNKALPDRFPFTWMLHGKMTQGARRYTTEQFKRTKRGVLICTDVAARGLDFKGVSDVIQMATTQNIEDYIHKIGRTGRAGQPGRATLFLSENELNFTKDLYTQKHIEFTSEVEYEMTAEAEEKLRGKLPEEDVREYVTSLMGFQSSVCDTYRMKKFDVIRSIVLLYRGFIEKEDATIYLSTRMFNNMALPGQLVPDYIEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEP2</accession>
    <name>A0A1A0HEP2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77794</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712874.1">
        <property type="nucleotide sequence ID" value="XM_018858768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEP2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEP2"/>
    <dbReference type="GeneID" id="30031744"/>
    <dbReference type="OrthoDB" id="434972at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF7">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="61"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="550" mass="62845" checksum="5F6F0C9BB925DAEA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEP3</accession>
    <name>A0A1A0HEP3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712967.1">
        <property type="nucleotide sequence ID" value="XM_018857201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEP3"/>
    <dbReference type="GeneID" id="30030177"/>
    <dbReference type="OrthoDB" id="4002254at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="73" mass="8226" checksum="4EAA71D782E070BC" modified="2016-10-05" version="1">MSIPESPQNPGPFPAQHGYVDVQAKTHKYKDEPTALPGQPSKAQQFISSKTDRQHQKKYGKKALNFLMKTLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HET3</accession>
    <name>A0A1A0HET3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_153251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712914.1">
        <property type="nucleotide sequence ID" value="XM_018854583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HET3"/>
    <dbReference type="GeneID" id="30027559"/>
    <dbReference type="OrthoDB" id="4086412at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="308" mass="34262" checksum="780966B59851294E" modified="2016-10-05" version="1">MTEKYILQENTLPEFIAEPVKYLSVVSKQGSVKNILGELLKQSHVYKGTVNDNVFDIAESIVGSGAQYFSLKLNKNRNTLKIDSRTPKTVTITLTEHVFNHHAYAARYMKFKKLQPAKGSSDYIVYHTVTANWNGPEKYSKDLYALVSEVFACAELVLLTDSVYAPKDEVGSLMDLHESAVVLEVKVFSPGEVDSGDSIAETPDLLKLDVDEHHEVYEYLSLVHLNSLPDYRNSHVGATSIYETPQAVHDLHLPLNSASLATVANVNPVLFYALANNPDVLSVQATTISGSYLLLILPDSSWMWTLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF69</accession>
    <name>A0A1A0HF69_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Carboxypeptidase S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_39567</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713126.1">
        <property type="nucleotide sequence ID" value="XM_018857155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF69"/>
    <dbReference type="STRING" id="869754.A0A1A0HF69"/>
    <dbReference type="GeneID" id="30030131"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000098">
        <property type="entry name" value="Gly-Xaa carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 18" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="17">
        <location>
            <begin position="265"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA22630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="559" mass="62080" checksum="D4D0FED78AF01073" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFI5</accession>
    <name>A0A1A0HFI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_92100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713242.1">
        <property type="nucleotide sequence ID" value="XM_018859334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFI5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFI5"/>
    <dbReference type="GeneID" id="30032309"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="35392" checksum="931ABDAD4F27612A" modified="2016-10-05" version="1">MLFLVPKFLVRLFLGSFALWIILSTAIPQLLLKFLEPQIRDLHFTQTAYPYNSLTTPAPPPIRNSTVVFVIGHPDDEVMFFSPSLVELAKPKHHNRIKLLCFSRGDAVDVSFGGIRAQELRSSARIIGVEPHDVVVLDEFQDGMDVQWKAADVAETLRQQIGRAAETTVVITFDEQGVSSHPNHISLYHGCKEYFQTHVEPHAAARLYVLKSLNFWEKYSFTALTNVELLVDLVLKFFLNTLKININVSFFSAYSGRSLSSIKFYSDLNMLSLSYAAMAYGHYSQMVWFRYGWLMFSRYLTFNHLIQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFL1</accession>
    <name>A0A1A0HFL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_9283</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="7">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713420.1">
        <property type="nucleotide sequence ID" value="XM_018859350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFL1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFL1"/>
    <dbReference type="GeneID" id="30032325"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035753">
        <property type="term" value="P:maintenance of DNA trinucleotide repeats"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903459">
        <property type="term" value="P:mitotic DNA replication lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045740">
        <property type="term" value="P:positive regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.150.10:FF:000006">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.70.10.10:FF:000001">
        <property type="entry name" value="Proliferating cell nuclear antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.150.10">
        <property type="entry name" value="DNA Polymerase III, subunit A, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00293">
        <property type="entry name" value="PCNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="10">
        <location>
            <begin position="128"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="259" mass="28864" checksum="6FAD9DFFB756EC77" modified="2016-10-05" version="1">MLEGKIDEAVLLKKIVESIKDCVKLCNFNCTEHGITVQAVDDSRVLLVSLLVGLSAFAEYRCDRDITLGIDLDSFSKIIKCGNNDDYLTLLAEDSPDNVVTIFEDKKRERVSEYSLKLMDIDSEFLRIDDMQYDSVVNMASSEFAKVVRDLKNLSESLNITVTKDAVKFSAEGESGTGSVVLKPFTDIDNPDQSVSVNLENSVDLTFGLKYLSDIIKATSLSNTITIKLADKTPALFEYKLEAGGYLRFYLAPKFDEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFN0</accession>
    <name>A0A1A0HFN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eisosome protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_9074</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EIS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713183.1">
        <property type="nucleotide sequence ID" value="XM_018859310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFN0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFN0"/>
    <dbReference type="GeneID" id="30032285"/>
    <dbReference type="OrthoDB" id="4070583at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024527">
        <property type="entry name" value="Eisosome1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28298:SF1">
        <property type="entry name" value="EISOSOME PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12757">
        <property type="entry name" value="Eisosome1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="67"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="577"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="450"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="531"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="577"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="610"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="79732" checksum="1345A53E44400BA1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFN8</accession>
    <name>A0A1A0HFN8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713297.1">
        <property type="nucleotide sequence ID" value="XM_018859328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFN8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFN8"/>
    <dbReference type="GeneID" id="30032303"/>
    <dbReference type="OrthoDB" id="4067583at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="759"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="890" mass="96673" checksum="B1C3483BEA6D2AE1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG10</accession>
    <name>A0A1A0HG10_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Actin/actin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9182</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin family. ARP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713313.1">
        <property type="nucleotide sequence ID" value="XM_018859330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG10"/>
    <dbReference type="STRING" id="869754.A0A1A0HG10"/>
    <dbReference type="GeneID" id="30032305"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10220">
        <property type="entry name" value="ASKHA_NBD_Arp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000050">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000005">
        <property type="entry name" value="Actin-related protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="392" mass="44290" checksum="286277D0715209EF" modified="2016-10-05" version="1">MSSAPIVLDQGTGFVKIGRAGTNFPDYTFPSLVGRPILRAEERNALVPDNIEIKDIMCGSEASEVRSLLQISYPMENGIIKNWEDMEHLWDYAFYERMKVPIQGEKILLTEPPMNPLKNRETMCDVMFEKYQFGGVYVAIQAVLALYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLTRRLDVAGRDVTRNLINLLLRRGYAFNRTADFETVRQIKEKLCYVSCDLALDLKLANETTTLVEQYELPDGRVIKLGSERFEAPECLFQPNLVDVEQAGVGETLFNTIQAADVDIRTSLYKAIVLSGGSSMYPGLPSRLERELKQLWLTRVLHGDASRLDKFKVRIEDPPRRRHMVFIGGAVLANIMANKEHMWISKQEWEEQGPRVLEKLGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG32</accession>
    <name>A0A1A0HG32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_76202</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713600.1">
        <property type="nucleotide sequence ID" value="XM_018858491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG32"/>
    <dbReference type="STRING" id="869754.A0A1A0HG32"/>
    <dbReference type="GeneID" id="30031467"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061957">
        <property type="term" value="C:NVT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120113">
        <property type="term" value="P:cytoplasm to vacuole targeting by the NVT pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000004">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="18"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="231"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="521"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="836" mass="92451" checksum="E3B0CACFBE32EFF1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG66</accession>
    <name>A0A1A0HG66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Copper resistance determinant 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713640.1">
        <property type="nucleotide sequence ID" value="XM_018856708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG66"/>
    <dbReference type="GeneID" id="30029684"/>
    <dbReference type="OrthoDB" id="273010at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057193">
        <property type="entry name" value="DUF7871"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40620">
        <property type="entry name" value="RESISTANCE PROTEIN CRD2, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04318)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40620:SF1">
        <property type="entry name" value="RESISTANCE PROTEIN CRD2, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04318)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25277">
        <property type="entry name" value="DUF7871"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7871" evidence="1">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="74" mass="7509" checksum="8B2798A555CCAE59" modified="2016-10-05" version="1">MACSAAECVCAKKSTCSCGKQAALHCNCEKAPVENAQPPKDGSCACGKRMKGQCTCGVSEASCAREGEVDFTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGT4</accession>
    <name>A0A1A0HGT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_9380</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713544.1">
        <property type="nucleotide sequence ID" value="XM_018859371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGT4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGT4"/>
    <dbReference type="GeneID" id="30032346"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000549">
        <property type="entry name" value="ABC transporter ATP-binding protein arb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000618">
        <property type="entry name" value="ATP-binding cassette (ABC) transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="81"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="392"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="151"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="609" mass="67980" checksum="E73E0E32B1EA997C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGZ8</accession>
    <name>A0A1A0HGZ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_103208</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713752.1">
        <property type="nucleotide sequence ID" value="XM_018853736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGZ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGZ8"/>
    <dbReference type="GeneID" id="30026712"/>
    <dbReference type="OrthoDB" id="5422795at2759"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000086">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000108">
        <property type="entry name" value="Glycerol kinase, glycosomal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 10 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="11">
        <location>
            <begin position="94"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="12">
        <location>
            <begin position="356"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="628" mass="69449" checksum="85DD8679A779E6C1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHA9</accession>
    <name>A0A1A0HHA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein kinase C-like 1</fullName>
            <ecNumber evidence="2">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">METBIDRAFT_37147</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713866.1">
        <property type="nucleotide sequence ID" value="XM_018856830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHA9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHA9"/>
    <dbReference type="GeneID" id="30029806"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20822">
        <property type="entry name" value="C1_ScPKC1-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20823">
        <property type="entry name" value="C1_ScPKC1-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08689">
        <property type="entry name" value="C2_fungal_Pkc1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11620">
        <property type="entry name" value="HR1_PKC-like_2_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05570">
        <property type="entry name" value="STKc_PKC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000101">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000014">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000034">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000474">
        <property type="entry name" value="Serine/threonine-protein kinase N2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037778">
        <property type="entry name" value="C2_fungal_PKC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037312">
        <property type="entry name" value="PKC-like_HR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02185">
        <property type="entry name" value="HR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="REM-1" evidence="24">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="24">
        <location>
            <begin position="105"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="20">
        <location>
            <begin position="188"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="411"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="22">
        <location>
            <begin position="477"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="754"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="23">
        <location>
            <begin position="1014"/>
            <end position="1081"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="544"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="677"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="723"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="565"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="584"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="649"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="680"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="783"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OBA23385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1081" mass="123553" checksum="1ED181830DF91E5D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHC3</accession>
    <name>A0A1A0HHC3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1708-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713881.1">
        <property type="nucleotide sequence ID" value="XM_018856699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHC3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHC3"/>
    <dbReference type="GeneID" id="30029675"/>
    <dbReference type="OrthoDB" id="3362494at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04401">
        <property type="entry name" value="RhoGAP_fMSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037508">
        <property type="entry name" value="Msb1/Mug8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012965">
        <property type="entry name" value="Msb1/Mug8_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28093:SF1">
        <property type="entry name" value="MORPHOGENESIS-RELATED PROTEIN MSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08101">
        <property type="entry name" value="Msb1-Mug8_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Meiotically up-regulated protein Msb1/Mug8" evidence="1">
        <location>
            <begin position="28"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="53012" checksum="7C1AEFEFA4321418" modified="2016-10-05" version="1" fragment="single">MAKDYDLDLPVLPAMHERNHQLISDSQLTRRKLQGLLHIMTAELKARGTKTPHIFLPFRSRVDDTKLNLLLRRIFPGGVMVDTSQKAIVRTILNDFDEFTLICGLKYLWCRLPNSEIIGWPVYLEFKRKEHEAGYPKDAFLTIMPKCLLSSSHASIVYDFLDLMISIASNSQYNHLNGRKVAKMASIWAFRSRPSSTSAFYDATLISERDFLDGLEAWKQMCNGLFHLLLSFLRAMLPDTEADTLNLPKTLQSLLITNSYPPPENSNSVKSVITIPCVSVRSTRKSKDPYELISKVRRSLSFDKKDFFLSIENFTILKNIFQKPSTADIVSTFSEESRRVISRLTADPIPSEFGLYPGWAQESLDLEEPDIPLYSEVRITSVTLQDYFIWTWLSSLGSDQTSNIKSIFGRSIVVEAGLRGFQKWMVLSETTIQPEEYISIFKSPKKDVFADSPKKCPLPPTPKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHC4</accession>
    <name>A0A1A0HHC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_9673</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713882.1">
        <property type="nucleotide sequence ID" value="XM_018859434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHC4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHC4"/>
    <dbReference type="GeneID" id="30032409"/>
    <dbReference type="OrthoDB" id="295274at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14687">
        <property type="entry name" value="bZIP_ATF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000053">
        <property type="entry name" value="BZIP transcription factor AtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051027">
        <property type="entry name" value="bZIP_transcription_factors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19304">
        <property type="entry name" value="CYCLIC-AMP RESPONSE ELEMENT BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BZIP" evidence="8">
        <location>
            <begin position="367"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="249"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="323"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="689" mass="74487" checksum="2728CD0AD5AF084F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHQ9</accession>
    <name>A0A1A0HHQ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SIS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713898.1">
        <property type="nucleotide sequence ID" value="XM_018856540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHQ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHQ9"/>
    <dbReference type="GeneID" id="30029516"/>
    <dbReference type="OrthoDB" id="1872003at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05014">
        <property type="entry name" value="SIS_Kpsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035474">
        <property type="entry name" value="SIS_Kpsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38418">
        <property type="entry name" value="SUGAR ISOMERASE, KPSF/GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38418:SF2">
        <property type="entry name" value="SUGAR ISOMERASE, KPSF_GUTQ (AFU_ORTHOLOGUE AFUA_6G08860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="52"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="374" mass="41076" checksum="DDEB970BD923A15C" modified="2016-10-05" version="1">MSAPEWQKLTFAALQSVKMTLTRQSEALKNLANHYVENIDTQLDMINSISLLYDAHSRGGKIVCCGIGKSYKIATKTVATLKSLTINADELHPAEALHGDLGLLRENDCIIFFTASGNTPELLQLLPHISPNIPIVLLTCSKESKLASHPQITSLLYAELPEHLKETVIHGLPAPTVSATLSLVLADAVSLALGEMVEVDHMKRKKTFSMKHPGGSIGSDLSHLNDNFNRGHSLTSSQSDMPHHGSYSSLLSLDQLRECFRTTSIASSDPESVSEEPQAVKPDSSFTSALMNAGKDACYRTTRSEFLSWSELDLLKNLTLYDYVMFESELKVLATDTEKVRLMYKDVSGLPVGFTGMNDFLTIFREVEIDVASV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHR3</accession>
    <name>A0A1A0HHR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetyl-CoA C-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_37295</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714180.1">
        <property type="nucleotide sequence ID" value="XM_018856853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHR3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHR3"/>
    <dbReference type="GeneID" id="30029829"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000007">
        <property type="entry name" value="acetyl-CoA acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF165">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="9">
        <location>
            <begin position="273"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="401" mass="41949" checksum="09498BFCE7AD795A" modified="2016-10-05" version="1">MQSVYVVSTARTPIGAFQGSLSSLTFSDLGAHAVKSALEKVPQIKPEMVDELVFGGVLQANVGQNPARQVALKAGLPEETISSTINKVCASGTKAIIIGAQAILTGTADIVVAGGAESMSNVPYYLPSARSGARFGDATMVDGVQKDGLLDVYEQKLMGVAAEKCAKDYDISRDEQDEFAKNSYLKAQKAQTDGKFSGEIAPVTIKGVRGKPDVVISEDEEPKNFNESRLKTSRTVFQKENGTVTAVNASKFNDGGAALVLASEAKVKELGLRPIARISGWGEAERSPIDFTVAPSLAIPKALKFAKLSYDDVDFYELNEAFAVVGLANAKICGLPQEKINAYGGAVALGHPLGCSGARIVVTLLSVLSQEGGRYGVAGICNGGGGASALVVEKLDSEARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHZ3</accession>
    <name>A0A1A0HHZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">NEDD8-activating enzyme E1 regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_34755</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the dimeric UBA3-ULA1 E1 enzyme.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713944.1">
        <property type="nucleotide sequence ID" value="XM_018856521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHZ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHZ3"/>
    <dbReference type="GeneID" id="30029497"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000475">
        <property type="entry name" value="NEDD8-activating enzyme E1 regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030667">
        <property type="entry name" value="APP-BP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF29">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039099">
        <property type="entry name" value="APP-BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="8">
        <location>
            <begin position="12"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="520" mass="58544" checksum="9DAD4FCE92D56573" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI21</accession>
    <name>A0A1A0HI21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_113431</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714290.1">
        <property type="nucleotide sequence ID" value="XM_018853964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI21"/>
    <dbReference type="GeneID" id="30026940"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="84" mass="9798" checksum="189B6BD6513C74F2" modified="2016-10-05" version="1">MRMWSWYVKFGNIDVSTNDSKPCFKSNSNCLKSDGTSEIAESWTKNANLLYILHSRLTNMVAFNTKKLERIPFTQYARNTQINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIB9</accession>
    <name>A0A1A0HIB9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">RhoGAP-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_34557</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714227.1">
        <property type="nucleotide sequence ID" value="XM_018856487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIB9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIB9"/>
    <dbReference type="GeneID" id="30029463"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000755">
        <property type="term" value="P:cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035024">
        <property type="term" value="P:negative regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090334">
        <property type="term" value="P:regulation of cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09391">
        <property type="entry name" value="LIM1_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09392">
        <property type="entry name" value="LIM2_Lrg1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF10">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="Multiheme cytochromes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="41"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="108"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="134"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="444"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="11">
        <location>
            <begin position="815"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="587"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="653"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1101" mass="123469" checksum="2B01E431A4795ABF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIG0</accession>
    <name>A0A1A0HIG0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CNH-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37766</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714276.1">
        <property type="nucleotide sequence ID" value="XM_018856912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIG0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIG0"/>
    <dbReference type="GeneID" id="30029888"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04435">
        <property type="entry name" value="DEP_fRom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041675">
        <property type="entry name" value="PH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF2">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15405">
        <property type="entry name" value="PH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="4">
        <location>
            <begin position="255"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="5">
        <location>
            <begin position="637"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="111"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="955" mass="109428" checksum="28B6D43A0577EF09" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIP2</accession>
    <name>A0A1A0HIP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714189.1">
        <property type="nucleotide sequence ID" value="XM_018855563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIP2"/>
    <dbReference type="GeneID" id="30028539"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20716" checksum="38C9C5FFD9024C25" modified="2016-10-05" version="1">MDRYELEKEPTELIKIIGSLNSKWDGKAYVGEAATSYTIKNDHVHTDVRTLRIGPDDSLEFALQGEKVLVVGGFLFFNDFHVFCCSAQNSRITLDATCRGGTKIMIKLDQENCSNILKKQCLFMYRQNFQTIESSIQQISCTFITLSFLLFLWLSYGFVLYNPFTSLLLLQYTFPTLESLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIS8</accession>
    <name>A0A1A0HIS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NGG1p interacting factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_76708</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714224.1">
        <property type="nucleotide sequence ID" value="XM_018858574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIS8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIS8"/>
    <dbReference type="GeneID" id="30031550"/>
    <dbReference type="OrthoDB" id="3345469at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="30430" checksum="676B46E9B7775260" modified="2016-10-05" version="1">MPSPTSSKTIARVTNAIQKLYPLHLADKSWDNTGLLVDSSSETAPESEEKCKILLTIDLTQSVASEAVKNNSNVVIAYHPFIFRGLKSITPKDPQQKSLLRLIQNNISVYSPHTAVDSAKGGVNDFLADGISGSFKESTREVILPDSKEEGCGMGRLVTLAQSAPLSELVNNVKSSLNLTHVQVATARKSDLTQAIRTIAICAGSGGSVFKGTHADLYFTGELSHHEALYFTETGSSVVACNHTNTERAFLRVLKSQLEKELPEAEIEVSQVDRDPFETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIT5</accession>
    <name>A0A1A0HIT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_117039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714550.1">
        <property type="nucleotide sequence ID" value="XM_018854067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIT5"/>
    <dbReference type="GeneID" id="30027043"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="159" mass="17796" checksum="4C9DA98BD963E039" modified="2016-10-05" version="1">MTFTCLGNKWPDVKCISALQPERWKPWSETRTMTAETKCMCGIFQDINNEHSERFLDCSNLSHFVQLSPESIRAPIGKASTPNHLSICNHKLLQQDLGPGPHCSLAPHRRTAANSCSALKTRSGLSNHIKSKKEQWIQTRSEIVATRPAAKHHPTLAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ86</accession>
    <name>A0A1A0HJ86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 20">Serine/threonine-protein kinase Tel1</fullName>
            <ecNumber evidence="5 20">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">METBIDRAFT_86246</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 20">Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone H2A to form H2AS128ph (gamma-H2A) at sites of DNA damage, involved in the regulation of DNA damage response mechanism. Required for the control of telomere length and genome stability.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with DNA double-strand breaks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Chromosome</location>
            <location evidence="2 20">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5 20"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714700.1">
        <property type="nucleotide sequence ID" value="XM_018859257.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HJ86"/>
    <dbReference type="GeneID" id="30032232"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05171">
        <property type="entry name" value="PIKKc_ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000032">
        <property type="entry name" value="Serine/threonine-protein kinase TEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038980">
        <property type="entry name" value="ATM_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044107">
        <property type="entry name" value="PIKKc_ATM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021668">
        <property type="entry name" value="TAN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37079:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11640">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01342">
        <property type="entry name" value="TAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 20" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7 20" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11 20" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="15 20" id="KW-0779">Telomere</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="22">
        <location>
            <begin position="1804"/>
            <end position="2407"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="21">
        <location>
            <begin position="2511"/>
            <end position="2821"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="23">
        <location>
            <begin position="2823"/>
            <end position="2855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU365027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OBA24219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2855" mass="324107" checksum="FAF1566DFF7520C2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ89</accession>
    <name>A0A1A0HJ89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_115410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714433.1">
        <property type="nucleotide sequence ID" value="XM_018854015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ89"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ89"/>
    <dbReference type="GeneID" id="30026991"/>
    <dbReference type="OrthoDB" id="4026687at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="366"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="689"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="783" mass="86246" checksum="04D1459AC2CF67CC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA4</accession>
    <name>A0A1A0HJA4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase M3A and M3B, thimet/oligopeptidase F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_81341</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714448.1">
        <property type="nucleotide sequence ID" value="XM_018859210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA4"/>
    <dbReference type="GeneID" id="30032186"/>
    <dbReference type="OrthoDB" id="534666at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF84">
        <property type="entry name" value="SACCHAROLYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="8">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="122" mass="13572" checksum="CFBD5638D7F849DC" modified="2016-10-05" version="1">MSLHTIETDAKLESLDIKSLWNSLREEVTLITNGGHDNIGYATFGHIAGGYESGYYGYLYSQVFATDIYYTHFKADPMNVASGLKYRDVILKNGGAKDILEILEELLGRKPNSNAFFAEILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJM1</accession>
    <name>A0A1A0HJM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_20990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714845.1">
        <property type="nucleotide sequence ID" value="XM_018855028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJM1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJM1"/>
    <dbReference type="GeneID" id="30028004"/>
    <dbReference type="OrthoDB" id="4085843at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="275"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="44008" checksum="1A6EB31B9375E400" modified="2016-10-05" version="1" fragment="single">EFWSVFVRDFSATYIRNHSPQEIEHKVLLGIPADMCFAIYSSMLNIPATVESHTYESLVRKAKLAKFEGGTKPDSDPADRIELSEVYWYCLKGNVHVASQVTGLLSPALIENFARILQLAEGISKHDALAILFKITERLATISKAGFKYKGSRCLEDFASEQFLHVTKQGINILAFFDTAFERFTEESMEKNILLNLFNMVLLEGPDYLLRLLCSAFERQKDRVLSLDGDALKEYIFSEEFFSAIDSETLEKSLNVDFPIIKYENEYSFMNSNSISGNDNEISNLQEANQELTLKLEGLKEKFVQLQITHEEIFGQNSEYASKLAHAREEKASLLIRKANLQQKYNQLTMQDNLSNTIKANKDISMENQNLEQQIEVMKKKVEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX7</accession>
    <name>A0A1A0HJX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Globin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_14195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714806.1">
        <property type="nucleotide sequence ID" value="XM_018854486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX7"/>
    <dbReference type="GeneID" id="30027462"/>
    <dbReference type="OrthoDB" id="436496at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase NAD(P)H activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071500">
        <property type="term" value="P:cellular response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046210">
        <property type="term" value="P:nitric oxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01040">
        <property type="entry name" value="Mb-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="entry name" value="Globins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044399">
        <property type="entry name" value="Mb-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF6">
        <property type="entry name" value="ABL201WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396">
        <property type="entry name" value="FLAVOHEMOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="Globin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Globin" evidence="1">
        <location>
            <begin position="84"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="303" mass="33584" checksum="2F374FB05C0D0E65" modified="2016-10-05" version="1" fragment="single">SSAYSGYRGNYKVTRVASRTSMASALYVKSQYRVSLDLSAKEISLLRYMWNQMLGEELAEEKSGRPSSALPIPGSLWAQAKEKPASSTPRHALGASSMFCSQLYLNLLTMDPGLEVEFPLLRHQAVSMAGVLSLAVNSFENLAQLDDYLVELGNRHSRILGIEPAQFELMGEAFIQTFHERFGTRFTHELEVLWIKFYMYLANSLLQFGLDPVMRPDYMHNGPYAVADLAAELIFTAESDDIMTMNNSMQREYTATDMSSMGSMPKASSFARHGSVGGTSPMDKTGVSLVLAQASQPARTKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJZ0</accession>
    <name>A0A1A0HJZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_120359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714683.1">
        <property type="nucleotide sequence ID" value="XM_018854151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJZ0"/>
    <dbReference type="GeneID" id="30027127"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508839" description="Secreted protein" evidence="1">
        <location>
            <begin position="27"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21299" checksum="EEE5E7D56725F98F" modified="2016-10-05" version="1">MNKSIYPRHSFLVVVYFCLHVSHFNAGFVSTVPGGPFVPHCAEKDSGGLAPYPLNPILRPHNSSAQLRDTCAQTVAGPYRSPWSRARVPAKGRRFFLTPLCSGLPGLVSRSPFADQFALLTDCEERGRNCRRTTLLGTCAHQKSPTAGPSSLRQFFCPLSGLDPGHFLYSDFIFGHLPSTLVFKKCRAHITHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK03</accession>
    <name>A0A1A0HK03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_131201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714812.1">
        <property type="nucleotide sequence ID" value="XM_018854307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK03"/>
    <dbReference type="GeneID" id="30027283"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="61" mass="6740" checksum="564B1BA7566AD5CA" modified="2016-10-05" version="1">MGKVSGSNPDSSIIFFAHILLSTQYNLELLLSIVQRVLPKKIKIAELMSRSEISAPRGLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK72</accession>
    <name>A0A1A0HK72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714882.1">
        <property type="nucleotide sequence ID" value="XM_018855682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK72"/>
    <dbReference type="STRING" id="869754.A0A1A0HK72"/>
    <dbReference type="GeneID" id="30028658"/>
    <dbReference type="OrthoDB" id="1879at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1090" mass="121727" checksum="7AC2E4E0F73D0E40" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK81</accession>
    <name>A0A1A0HK81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell wall assembly and cell proliferation coordinating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_16196</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KNR4/SMI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714911.1">
        <property type="nucleotide sequence ID" value="XM_018854631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK81"/>
    <dbReference type="STRING" id="869754.A0A1A0HK81"/>
    <dbReference type="GeneID" id="30027607"/>
    <dbReference type="OrthoDB" id="2305498at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070880">
        <property type="term" value="P:fungal-type cell wall beta-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1580.10">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009203">
        <property type="entry name" value="Knr4/Smi1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018958">
        <property type="entry name" value="Knr4/Smi1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037883">
        <property type="entry name" value="Knr4/Smi1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051873">
        <property type="entry name" value="KNR4/SMI1_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47432:SF1">
        <property type="entry name" value="CELL WALL ASSEMBLY REGULATOR SMI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09346">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017023">
        <property type="entry name" value="KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00860">
        <property type="entry name" value="SMI1_KNR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160631">
        <property type="entry name" value="SMI1/KNR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knr4/Smi1-like" evidence="2">
        <location>
            <begin position="95"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="345" mass="38835" checksum="FD20989D68FF5D6C" modified="2016-10-05" version="1" fragment="single">MTLSLQMKEFFYCLSATDKYSDFDSRKSFNRVNQPEIETSLLGATHANDSTSSLSKAANLASSEEGVHEVRLAWRHIKKWLHKHSEDMNNSLLAPCTEADINELQKDLDCEFPACLKEFLRLTDGQSQFNENGCCGLFYGLRLLSIDEIAVMTESWRKVHESILQNTEFSGLQKYAVNSSFADNNLGTSAGFKMRPSEMKVNQNEFPEQYSVPPHTVHSIYAHSQWIPLINDETGNFIGIDLSPGASDSSAIPAAKWGQVILFGRDFDIKYKIADTFGDFLLIFANDLEKGNWDLRSSSDNEDMVSGVDAELVFVDHETKKELPYLNVLKDRATTLWISSLSAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK87</accession>
    <name>A0A1A0HK87_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714897.1">
        <property type="nucleotide sequence ID" value="XM_018855686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK87"/>
    <dbReference type="GeneID" id="30028662"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="51" mass="5466" checksum="0B4F6C7EEBFB44B8" modified="2016-10-05" version="1">MYQNRDCSSLLSSVIPLSYTSPRCPTSGLPGGHIEILDFRCQVKSPAGLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKG4</accession>
    <name>A0A1A0HKG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_36259</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714779.1">
        <property type="nucleotide sequence ID" value="XM_018856713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKG4"/>
    <dbReference type="STRING" id="869754.A0A1A0HKG4"/>
    <dbReference type="GeneID" id="30029689"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="407" mass="46331" checksum="319D80CA7ACB66DE" modified="2016-10-05" version="1">MASSRSNARLIRFGIFALVLFFCGYILTKGSSYPAGAPGGASTAAKNAQNAVSGGQAAAPAAAAAAGPLAGNGAGAAFLIPPGKVKATFVTLARNSDLQGLTQSITHIEERFNSKYHYDWVFLNDAPFDEDFKTAVQGMVSGQAKFGLIPQEHWSYPEWIDKEKAALGREEMVAKNVIYGGSESYRHMCRFESGFFFRHALLEDYEYYWRVEPDIKIHCDIDYDLFQYMKDNDKWYGWTISLPEYRETIPTLWDTTRKFMEENPQYLAKDNNMAWISNDKGNTYNGCHFWSNFEIGALGFWKSEAYLKYFDALDKAGGFFYERWGDAPVHSIAASLFMPRDKLHFFQDVGYFHTPFNNCPVDQELRAQKKCLCEPEQDFTWRPYSCTPKYFTLNGLKRPKGWENFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ80</accession>
    <name>A0A1A0GZ80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine dehydratase</fullName>
            <ecNumber evidence="12">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_80195</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709330.1">
        <property type="nucleotide sequence ID" value="XM_018859191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ80"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ80"/>
    <dbReference type="GeneID" id="30032167"/>
    <dbReference type="OrthoDB" id="4418812at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901705">
        <property type="term" value="P:L-isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04906">
        <property type="entry name" value="ACT_ThrD-I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04907">
        <property type="entry name" value="ACT_ThrD-I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01562">
        <property type="entry name" value="Thr-dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1020.10:FF:000001">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000008">
        <property type="entry name" value="L-threonine dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="entry name" value="Biosynthetic Threonine Deaminase, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006674">
        <property type="entry name" value="PRK09224.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF11">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="13">
        <location>
            <begin position="471"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA17033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="552" mass="61593" checksum="4F85457A2279B6C5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1R6</accession>
    <name>A0A1A0H1R6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase/transpeptidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709369.1">
        <property type="nucleotide sequence ID" value="XM_018856390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1R6"/>
    <dbReference type="STRING" id="869754.A0A1A0H1R6"/>
    <dbReference type="GeneID" id="30029366"/>
    <dbReference type="OrthoDB" id="428260at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050789">
        <property type="entry name" value="Diverse_Enzym_Activities"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283:SF3">
        <property type="entry name" value="BETA-LACTAMASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G07500)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43283">
        <property type="entry name" value="BETA-LACTAMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="1">
        <location>
            <begin position="23"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="395" mass="43876" checksum="33BD46ACD07EA1F8" modified="2016-10-05" version="1">MLKLEDICSKYVTAKDGADVPDINAVIAGYTDRNQTKFLSAKGVKNINTGARASTDDILCFFSCTKPMTAMAVLILWERGRIELDVPAKRYLPVLSRFGVIENDQIDPKTGDLKSVPKLPDTDITIRHLLLHTSGFAYTFTNVDYLKIISKKRLFSPLLQLFSPSNMPLVFEPGSDWIYGHSYDWLGLIVQAVSGKKLSEFLIENVFSRAAMTSCTFKVSEPLNMICLHDKSKGSLKLYEKKLSVPLISEIDMGGQGCFGTVGDYLKFMRIWLNHGISPDTGARILTKKTVEYAVQNHLPAGQATNFPIPFSDQSNGYSLAGCGYSGKEVETGRPVGALYWAGLGNLYFWMDIKNGCAGFWGCQILPYMDSSCIKGFKEFEKAVYLKYVGSQTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1U2</accession>
    <name>A0A1A0H1U2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709394.1">
        <property type="nucleotide sequence ID" value="XM_018854447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1U2"/>
    <dbReference type="GeneID" id="30027423"/>
    <dbReference type="OrthoDB" id="4021450at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="520" mass="58930" checksum="ADA6F82624F90733" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X1</accession>
    <name>A0A1A0H4X1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable endonuclease LCL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Probable endonuclease lcl3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_47363</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LCL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709492.1">
        <property type="nucleotide sequence ID" value="XM_018858156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X1"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X1"/>
    <dbReference type="GeneID" id="30031132"/>
    <dbReference type="OrthoDB" id="430293at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000035">
        <property type="entry name" value="Probable endonuclease LCL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="TNase-like" evidence="16">
        <location>
            <begin position="56"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA18957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="228" mass="26204" checksum="2AFC8DF27B866689" modified="2016-10-05" version="1">MSQAGQETISIYHPKVLLLSGGVTAGAFFSFRFYGRFIRRRRTYLDLTPRILDGQQKLRGRVTRVGDGDNFRFYHTPGGALMGWGWARKVPEKRKDLKNETLMIRLCGVDAPERAHFGNPSQPFSEEALWWLQKYLMGRSVTITPYSIDQYKRVVARAQVWKLTGKKDVSAEMLRNGLAVVYEANQGAEYGENEHWYRKLEAKAKAKKRGLWSQGRKLVTPGDFKKNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H555</accession>
    <name>A0A1A0H555_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33671</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709588.1">
        <property type="nucleotide sequence ID" value="XM_018856375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H555"/>
    <dbReference type="STRING" id="869754.A0A1A0H555"/>
    <dbReference type="GeneID" id="30029351"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444427" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="22"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="530"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="568"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="637" mass="72197" checksum="52DA05988ABDA4C6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5C0</accession>
    <name>A0A1A0H5C0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ZIP zinc/iron transport family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_46787</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709811.1">
        <property type="nucleotide sequence ID" value="XM_018858079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5C0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5C0"/>
    <dbReference type="GeneID" id="30031055"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF44">
        <property type="entry name" value="PROTEIN ZNTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="347" mass="37895" checksum="94682D7426D1119F" modified="2016-10-05" version="1" fragment="single">TSKVSMLSTCGTASEFNGEYMGARIASVFVLLALSAAGSFFPLLAINYKFFRIPDIVIFLTRYFGSGVIVATAFIHLLGESQTSLSHPCVGEIFLEYSWSAAFSLMGVVLMFTIEAFTHRRLDRRNRLALLLCGMGSHDHEKAESHEETTEICVCTGESSNEDSGTTTEVPSTAEAPEKAPKEAVIKALVNIYLLEFGIVFHSIFVGLSLAIAGEGFKTLFVAISFHQFFEGMGIGARFATAKWPEHLKRIPWYLSAAYTVTTPIGIAAGLGVRNLYLENSPVSLIVVGVFDGFCSGLLIYNSLVELMARDFISDPLMKNISNFRFALAYGMFLTGALMMSIVGKWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5E0</accession>
    <name>A0A1A0H5E0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_27756</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709783.1">
        <property type="nucleotide sequence ID" value="XM_018855343.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5E0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5E0"/>
    <dbReference type="GeneID" id="30028319"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057614">
        <property type="entry name" value="PH_PKH3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25347">
        <property type="entry name" value="PH_PKH3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="8"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="401"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="468"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="543"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="792"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="543"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="800"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="844" mass="95721" checksum="3F100396536CAC1B" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5G5</accession>
    <name>A0A1A0H5G5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="4 7">2.1.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79720</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PanB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="4 7"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709695.1">
        <property type="nucleotide sequence ID" value="XM_018859117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5G5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5G5"/>
    <dbReference type="GeneID" id="30032093"/>
    <dbReference type="OrthoDB" id="425211at2759"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000003">
        <property type="entry name" value="3-methyl-2-oxobutanoate hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001452">
        <property type="entry name" value="PRK00311.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881:SF0">
        <property type="entry name" value="3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="32706" checksum="C02DE067C3D84917" modified="2016-10-05" version="1">MFRQTARSFSAARSAFSAYSQQPRKTLKHLNELYQQKSPISVVTAWDYLTAQIAEKADIDITLVGDSLAMVALGYEDTNEIGLDEFLYHVRAVARGNRSLFVVADVPFGTFEASDADAVRTAVALVKNGRAQGVKIEGGASLAPAIQKIVQAGVPVMGHVGLAPQKHHATGGYRLQGNSLQSAVQILDDCRALEAAGVFALVLECVPNKFAEIVTALVSVPVIGIGAGPHVSGQVLVMADMLGMGDKPAAKFVKQYASFFADAHAALAQYKADLATGAFPDPDIHGYKMKGDVLRKVREHASAARKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5R0</accession>
    <name>A0A1A0H5R0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33117</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709952.1">
        <property type="nucleotide sequence ID" value="XM_018856230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5R0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5R0"/>
    <dbReference type="GeneID" id="30029206"/>
    <dbReference type="OrthoDB" id="4022975at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="476" mass="53759" checksum="668A45BCA9C0E893" modified="2016-10-05" version="1">MSSSQKAESPRPFLPTRRHSNFEDQKSYHADLKSTLRLEQSSLCESLEKEKPHERKSSKSSESLSYTNSLTSPLPTPEEYISETIEIPIHKAYSETIVSQMLLLRFEQEKTKQIKSKIELGQIVAQLLKECEAHAISTQLIHRLFSDDNDEIIKNNVEKLTQQFASGNTPKSSGEEIKTSETNKMNDIVSKPSHEITNPMNSLSNHPQASIQENLLHTSSIDITLFTKKHPESNPTPLGDQNELLKSQKTVSDPQPRNYTEISSSETKEEALEVTQTLLTMGSKNQSERPVMAQPILPLYYRKHSRYLLPQVASGSQPSHIPELGLLLLCHNHSKIGFDSSSQYLKNGMLYREKYSQQTLLLMNSSSQQRNYEHQGQQFQQQIRPLYHVESLVPGVVPSTGNIATLLLPPPFTVGAGTHLQLWAATPLNPQTRRRRLEEDTHTQRKRRGSKNGIVFMISTPKNPPAQKYSRSSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5W3</accession>
    <name>A0A1A0H5W3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709877.1">
        <property type="nucleotide sequence ID" value="XM_018858062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5W3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5W3"/>
    <dbReference type="GeneID" id="30031038"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF68">
        <property type="entry name" value="GLYCOSIDASE CRH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291662" description="GH16 domain-containing protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="2">
        <location>
            <begin position="23"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="279" mass="30881" checksum="6A9E635BD2557E83" modified="2016-10-05" version="1">MKIHGLSLVLNLFSILFLACTSSAYKEFCNPQKNSSCIARNRALQEPVKDPLTNNSAAFAGSNSCPLLCPCDNGTNFGILQRFDNPRLSSTEYILYGRAEADIMGAPGQGIISSFYLQSDDLDEIDIAEIFGSAPAVYQTNFFVKGDVANYADGAYHRVTNSTTQNFHRYGVQWNRLEIEWTLDGQVVRHRAGGTLPSSPMRVILSLWVGGDEANDPGTVQWAGGPTDFADAPFSMYARNIYVENYSDGGCYVYETNLTVSIEDECRQSQYFVKWNSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H613</accession>
    <name>A0A1A0H613_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UPF0220-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_214282</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0220 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709999.1">
        <property type="nucleotide sequence ID" value="XM_018855073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H613"/>
    <dbReference type="STRING" id="869754.A0A1A0H613"/>
    <dbReference type="GeneID" id="30028049"/>
    <dbReference type="OrthoDB" id="268928at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034424">
        <property type="term" value="C:Vps55/Vps68 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007919">
        <property type="entry name" value="UPF0220"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13180">
        <property type="entry name" value="SMALL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05255">
        <property type="entry name" value="UPF0220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="177" mass="19454" checksum="AD746793C082961F" modified="2016-10-05" version="1">MEQPRSLFRLNAALLRLPKSQKLRSIGIYVAGALFAIGLWSMVDAAIYSKTVNASVVHVTFVDWIPFICSMLGMLIVNSIEKSNLFSEGQNLFLGDGSGNAWAARVILFFGFSLLAGGLAGSFMVFILKFLMKQYTFPTLGMGVSNIICNGSIMLSCIILWLSQNIEDEYSYSLALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H622</accession>
    <name>A0A1A0H622_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_222998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709937.1">
        <property type="nucleotide sequence ID" value="XM_018855155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H622"/>
    <dbReference type="GeneID" id="30028131"/>
    <dbReference type="OrthoDB" id="3162794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00646">
        <property type="entry name" value="F-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="582" mass="65800" checksum="C8AF3FA37C24D3C9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H648</accession>
    <name>A0A1A0H648_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cargo-transport protein YPP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33550</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in endocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the YPP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709914.1">
        <property type="nucleotide sequence ID" value="XM_018856328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H648"/>
    <dbReference type="STRING" id="869754.A0A1A0H648"/>
    <dbReference type="GeneID" id="30029304"/>
    <dbReference type="OrthoDB" id="29013at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23270">
        <property type="entry name" value="YPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051722">
        <property type="entry name" value="Endocytosis_PI4K-reg_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083:SF464">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT DOMAIN 7, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23083">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN, TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="922" mass="105751" checksum="B4D9942CCAEA8C61" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6K5</accession>
    <name>A0A1A0H6K5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">EF-hand</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710071.1">
        <property type="nucleotide sequence ID" value="XM_018859087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6K5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6K5"/>
    <dbReference type="GeneID" id="30032063"/>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="17476" checksum="B100302B6B517648" modified="2016-10-05" version="1">MASLLSLSNEQKSQVRNAFTLIDSESKDGSISMSDLANIHKTLGLELPSTEVLESMLGGKELVSFAQFSQIIAGELAKIDDRTTISNALRVFASEDAKSNLLIDVDELKEACCSVQLGDIGSGDHRLSRASFDSLVAGFVREQMDGKKVFNALNWISAYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Z4</accession>
    <name>A0A1A0H6Z4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cystathionine gamma-lyase</fullName>
            <ecNumber evidence="4">4.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Gamma-cystathionase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_45044</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.1" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710394.1">
        <property type="nucleotide sequence ID" value="XM_018857867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Z4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Z4"/>
    <dbReference type="GeneID" id="30030843"/>
    <dbReference type="OrthoDB" id="3512640at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004123">
        <property type="term" value="F:cystathionine gamma-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904828">
        <property type="term" value="P:positive regulation of hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000043">
        <property type="entry name" value="Cystathionine gamma-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000008">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF15">
        <property type="entry name" value="CYSTATHIONINE GAMMA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="5 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="398" mass="42203" checksum="419DF6CC840E8609" modified="2016-10-05" version="1">MTVDNTIQYGFGTHAVHAGAAADPHTGAVIAPISLSTTFAQSEPSKPVGIYEYSRSANPNRDNFEQAVATLEKAKYAVALASGSATTSLILQSLPMGSHVISGGDVYGGTHRYFTKVANTHGVEVSFVGDLAKDVQSALRPETALVWLETPSNPTLSVTDIAAVSAVLKAHNEKHGKNAFMVVDNTFLSPYVSNPLVHGADVVIHSITKYINGHSDVVGGVLATSSDVLHEKFRFLQNAIGSVPSPFDSWLAHRGLKTLHLRVRQASSSAQKIAEYLSQHEGIISVNYPGLKSHPNHAVVLKQHRDGLGGGMISFRIKGGADAAAKFTSTTQLFTLAESLGGIESLIEVPAVMTHGGIPKAEREANGVFDDLVRVSVGIEETDDLLNDIKQALDKLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H707</accession>
    <name>A0A1A0H707_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative ADP-ribosylation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_12918</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710409.1">
        <property type="nucleotide sequence ID" value="XM_018854279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H707"/>
    <dbReference type="STRING" id="869754.A0A1A0H707"/>
    <dbReference type="GeneID" id="30027255"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010513">
        <property type="term" value="P:positive regulation of phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000412">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="175" mass="19637" checksum="5ED242ED74C49F76" modified="2016-10-05" version="1">MGVLVSRLFKNREMRILMLGLDNAGKTTILYKMKLGKTSKTVPTVGFNVETVKHRNISFAVWDCGGQERIRPLWRHYFTGTNALIYVVDSSDLDRLDESRDELMRVISDKELASCLLIVLANKQDVPGAIKPKDLIERFDMSSLSGQHTWSVIPTIAVDGTGLGETLNWISSHSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H722</accession>
    <name>A0A1A0H722_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine/threonine-protein kinase RIO2</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Serine/threonine-protein kinase rio2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_44475</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710424.1">
        <property type="nucleotide sequence ID" value="XM_018857799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H722"/>
    <dbReference type="STRING" id="869754.A0A1A0H722"/>
    <dbReference type="GeneID" id="30030775"/>
    <dbReference type="OrthoDB" id="10258631at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046830">
        <property type="term" value="P:positive regulation of RNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000053">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000566">
        <property type="entry name" value="Serine/threonine-protein kinase rio2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000052">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="16">
        <location>
            <begin position="66"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA19899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="423" mass="48875" checksum="BF7FCBD9F1E99614" modified="2016-10-05" version="1">MKLDTSHMRYLTADDFRVLHAIEMGSRNHELVPTQMIHSIGGLKSPSGTNRALGDLAKLKLVARLRNAKYDGFRITYLGYDYLALKLMLNRNTLYSVGCTIGVGKESDIYSVSDPNGQQKVLKIHRLGRTSFKTVKNNRDYLKNKLTSSWMYLSRLAAEKEYQFMTVLYDNGFIVPTPYDYLRHCVMMEWIRGYTMKHMKKYPSYKKLYSDLMNFIVKLANHGLIHCDFNEFNIIIRDPEDAKDLEFDFVVIDFPQCVSIEHVDAKFYFQRDVDGIRSFFEKKFNYTPEDDSTMFDTEGYGEGYKHAYPDFGRDVRRVKELDAEVKASGWTKKKNREDRDLEAAVEGMRAIDLDEAQEESDSADADEEGLEAELEDEYEDELDILNSSEEVVDDEENEKIIEAISAGGKNLKVDNLGNYILDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H758</accession>
    <name>A0A1A0H758_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">Homoaconitase, mitochondrial</fullName>
            <ecNumber evidence="5 17">4.2.1.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 17">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_79110</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 17">Catalyzes the reversible hydration of cis-homoaconitate to (2R,3S)-homoisocitrate, a step in the alpha-aminoadipate pathway for lysine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="EC" id="4.2.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="17">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-alpha-aminoadipate from 2-oxoglutarate: step 3/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 17">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.36" evidence="5 17"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710262.1">
        <property type="nucleotide sequence ID" value="XM_018859002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H758"/>
    <dbReference type="STRING" id="869754.A0A1A0H758"/>
    <dbReference type="GeneID" id="30031978"/>
    <dbReference type="OrthoDB" id="10262323at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER01027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01674">
        <property type="entry name" value="Homoaconitase_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000013">
        <property type="entry name" value="Homoaconitase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004418">
        <property type="entry name" value="Homoaconitase_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039386">
        <property type="entry name" value="Homoaconitase_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00139">
        <property type="entry name" value="h_aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF2">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 17" id="KW-0408">Iron</keyword>
    <keyword evidence="11 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="13 17" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="18">
        <location>
            <begin position="32"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="19">
        <location>
            <begin position="487"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA19737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="684" mass="73830" checksum="4BE7D470A1956251" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H759</accession>
    <name>A0A1A0H759_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_88326</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710386.1">
        <property type="nucleotide sequence ID" value="XM_018859290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H759"/>
    <dbReference type="STRING" id="869754.A0A1A0H759"/>
    <dbReference type="GeneID" id="30032265"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="5">
        <location>
            <begin position="35"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="216" mass="23814" checksum="A3608C36E6D10DC8" modified="2016-10-05" version="1">MLPTPYVRNLDERVYDPAEDSFLLLDALEECLPQLRLRQFPVVCEIGAGSGIVSAFLKMHVFPHGLFFATDVNPAACDTIVATVRENDTASRFATLDVFQMSLALALRSNHIDVLVFNPPYVPAEAVPERPAAAADPAWLDLALLGGSDGMVVTWQVLDGLHRLLAADGVAYILFCARNKPDSVARVMEDRGWAVRTVLHRKAGWEVLSVLEFRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H763</accession>
    <name>A0A1A0H763_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Serine/threonine-protein kinase BUR1</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Serine/threonine-protein kinase bur1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_79203</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710391.1">
        <property type="nucleotide sequence ID" value="XM_018859019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H763"/>
    <dbReference type="STRING" id="869754.A0A1A0H763"/>
    <dbReference type="GeneID" id="30031995"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070691">
        <property type="term" value="C:P-TEFb complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070693">
        <property type="term" value="C:P-TEFb-cap methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000415">
        <property type="entry name" value="CMGC/CDK/CRK7 protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF233">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="7"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00041018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA19866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="42679" checksum="6E39ACF1E72845D0" modified="2016-10-05" version="1">MARLSQFEIIKKIGQGTFGVVQKARDRKLGKLVALKQLINHSAKEGFPITAMREITILKRLSHKNVLKIEGMIHEEPKTASPQDIISQRGVFYTVSPYMSSDLVGLLENPAIDLDLPTKKCILQQLLQGIDYVHQQRYLHRDIKAANILMDPTGVVKIADFGLARVYHGPIPLKGKGPGGGERNYTALVVTRWYRPPELLLGERKYTTAVDMWGVGCVFAELFTKKPILVGSSDLNQSQIIFDLVGPPNRDNWPDASLLPNKPDLNIGLTCRRTLETRFEPLLGNVGTGLLGKLLALDPKKRYNALDALDHEYFATDPKPILPEQIRKFEESHEIDRERFKRLRSLAEPRLIQFADKRRLRQNYSTWTLATAGRKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H775</accession>
    <name>A0A1A0H775_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_27976</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710469.1">
        <property type="nucleotide sequence ID" value="XM_018855345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H775"/>
    <dbReference type="STRING" id="869754.A0A1A0H775"/>
    <dbReference type="GeneID" id="30028321"/>
    <dbReference type="OrthoDB" id="422427at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051711">
        <property type="entry name" value="Stress_Response_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540:SF1">
        <property type="entry name" value="ACTIVATOR OF STRESS GENES 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47540">
        <property type="entry name" value="THIAMINE REPRESSIBLE GENES REGULATORY PROTEIN THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="532"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="791" mass="90779" checksum="E6535A0E9E70BB14" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H786</accession>
    <name>A0A1A0H786_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45575</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710365.1">
        <property type="nucleotide sequence ID" value="XM_018857933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H786"/>
    <dbReference type="STRING" id="869754.A0A1A0H786"/>
    <dbReference type="GeneID" id="30030909"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072517">
        <property type="term" value="C:host cell viral assembly compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052629">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="516"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="545"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="122"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="611" mass="70161" checksum="D278E34D9F88698C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7B6</accession>
    <name>A0A1A0H7B6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NPR2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32864</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NPR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710318.1">
        <property type="nucleotide sequence ID" value="XM_018856149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7B6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7B6"/>
    <dbReference type="GeneID" id="30029125"/>
    <dbReference type="OrthoDB" id="338854at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000785">
        <property type="term" value="P:regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015840">
        <property type="term" value="P:urea transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009348">
        <property type="entry name" value="NPR2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991:SF10">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12991">
        <property type="entry name" value="NITROGEN PERMEASE REGULATOR 2/TUMOR SUPPRESSOR CANDIDATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06218">
        <property type="entry name" value="NPR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="319"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="525"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="586"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="338"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="540"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="594"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="658" mass="73666" checksum="5B88CEA005D850E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7G3</accession>
    <name>A0A1A0H7G3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710491.1">
        <property type="nucleotide sequence ID" value="XM_018854289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7G3"/>
    <dbReference type="GeneID" id="30027265"/>
    <dbReference type="OrthoDB" id="10495519at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291686" evidence="1">
        <location>
            <begin position="18"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="27464" checksum="9638B26802A7A60C" modified="2016-10-05" version="1">MLFFSPALLLWLALALGAENRLQVQPLDMHHISSVDDLVPDDFGKHSVIFVKNESSVILPKFLHEKAHDPTFFQELTAIFNPKRLKDRIYHSKHYVQTQFSRQNLPANASEDFPLDGCFDNSLSETTSLVARSYEKTTLRQGSLNLFASLLGVEVDAGSNAGFTDISEEKIMCTVNPGRKLQLETNLLHKEMKLHRERNITIHAGSSGAPLLNFSEWKNVKGPNELEITIRTLSCVTDSQKIQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K7</accession>
    <name>A0A1A0H7K7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_33047</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710604.1">
        <property type="nucleotide sequence ID" value="XM_018856204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K7"/>
    <dbReference type="GeneID" id="30029180"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034737">
        <property type="term" value="F:ergosterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF23">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="413"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="501"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="556"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="576" mass="67240" checksum="5E98646FBAEE759A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7P7</accession>
    <name>A0A1A0H7P7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NADPH:adrenodoxin oxidoreductase, mitochondrial</fullName>
            <ecNumber evidence="8">1.18.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_12953</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>2 reduced [adrenodoxin] + NADP(+) + H(+) = 2 oxidized [adrenodoxin] + NADPH</text>
            <dbReference type="Rhea" id="RHEA:42312"/>
            <dbReference type="Rhea" id="RHEA-COMP:9998"/>
            <dbReference type="Rhea" id="RHEA-COMP:9999"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8 9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ferredoxin--NADP reductase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.6" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710448.1">
        <property type="nucleotide sequence ID" value="XM_018854286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7P7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7P7"/>
    <dbReference type="GeneID" id="30027262"/>
    <dbReference type="OrthoDB" id="333024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055275">
        <property type="entry name" value="Ferredox_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021163">
        <property type="entry name" value="Ferredox_Rdtase_adrenod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48467">
        <property type="entry name" value="GLUTAMATE SYNTHASE 1 [NADH], CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48467:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE 1 [NADH], CHLOROPLASTIC-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000362">
        <property type="entry name" value="FNR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8 10" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="22"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="177"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="222"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="418"/>
            <end position="420"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="56246" checksum="D7FE2D3209F73F80" modified="2016-10-05" version="1">MTSMICWPIARRGITTSRVANYNVAIVGAGPAGFYTAHHLLHKSKHMPITVDFFDRLPTPYGLSRYGVAPDHPEVKNCEEYMNKLWTDFSDKVRFFGNVNIGKDILLAQLEARYNSIVLSYGCTAFDIKLDIPGSSSPGVISAREFVNWYNGHPDSKQGSHFIPPLEKVRNVTIIGNGNVAIDIARVLLADPKSHWAPTDITSEAVELLSRSKVERVNIVARRGILESAFTNKEIRELLEISKELNIKFLPIPENVLAPIRQKAKLLARVEKRKFSILDKASSQATTPGESSKEWCLEFQLSPKQFVPSDEDAQLLKSTVFEVNELQEDSLTKKVTNKGTGKEIRIENDLVILSIGYKGEELEGFTASELEFTQKDNRIANNEGRILRSGAAAAGKEEEFTYKRGWYVSGWIKNGPKGVIATTMMDSFDTAEKILEDLSNEIYNVPNESSDIEKILPSPYVSWNGWKTIDLFELRQGRLLGKSRSKLRTINEMVQASRDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7T2</accession>
    <name>A0A1A0H7T2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mitochondrial protein with role in iron accumulation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_79280</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mitochondrial metal transporter involved in mitochondrial iron accumulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710483.1">
        <property type="nucleotide sequence ID" value="XM_018859030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7T2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7T2"/>
    <dbReference type="GeneID" id="30032006"/>
    <dbReference type="OrthoDB" id="435980at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000013">
        <property type="entry name" value="Cation efflux family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0409">Iron storage</keyword>
    <keyword evidence="5" id="KW-0410">Iron transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="13">
        <location>
            <begin position="79"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="13">
        <location>
            <begin position="223"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="410" mass="44189" checksum="FE70264BECAE5830" modified="2016-10-05" version="1">MASGSKLPKQSAGVSGVGKQAPTQSTQGHSHTHEDRHDDTGNLHSHDHKSLFHTHTHSNHHNELLGKGFLTNPAVRITWIGLVVNVVMAGSKAVGGVYFHSQALIADAIHSVSDMVADFLTLATVNVALKEGSYTKYPLGYGKLESLGTFAVAGVLLFAGFTVGWSSLLQIFEIFLPSHIYEQLLVLQVHSHSHSLGPDIPADHSHSHASVGLSERVAPNINAAWIALASIGVKEILYRKTMSVAHQTNSKILVANAWHHRVDSLTAGVAFITVTSGVLLGWSWLDAVGGLVVSALIMKAGSGSLKVAWYELIDRGSDPKSEDYTQIRLIVDTEVKNVSQAMKTGFQLTDFSVLTAGARTNLILKLTTDKDIALAAINDLEHDLVAGIREKDKTVGKVFVEYSLNDDKHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7W8</accession>
    <name>A0A1A0H7W8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Ubiquitin-related modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">URM1</name>
        <name evidence="7" type="ORF">METBIDRAFT_13014</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH) at its C-terminus by the MOCS3 homolog UBA4. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. Also acts as a ubiquitin-like protein (UBL) that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as AHP1. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 6">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of UBA4, then thiocarboxylated (-COSH) via the rhodanese domain of UBA4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the URM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710512.1">
        <property type="nucleotide sequence ID" value="XM_018854292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7W8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7W8"/>
    <dbReference type="GeneID" id="30027268"/>
    <dbReference type="OrthoDB" id="10248987at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007114">
        <property type="term" value="P:cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01764">
        <property type="entry name" value="Ubl_Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03048">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14986">
        <property type="entry name" value="RURM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037379">
        <property type="entry name" value="Ubiquitin-related_modifier_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="modified residue" description="1-thioglycine" evidence="5">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)" evidence="5">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="100" mass="10973" checksum="B7CE33C19DECA38A" modified="2016-10-05" version="1">MIKVKVEFLGGLDVISNGIREHKVTLDGEEGDVLMGDLIKHVTATIVCDPKDIPVFIEEDTVRPGILVLINDTDWELEGMEDYVVEAGDVFTFTSTLHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8A4</accession>
    <name>A0A1A0H8A4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_43952</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710732.1">
        <property type="nucleotide sequence ID" value="XM_018857723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8A4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8A4"/>
    <dbReference type="GeneID" id="30030699"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="306" mass="34132" checksum="C1AE7947E0C329EF" modified="2016-10-05" version="1">MYNPYALAVDESLGQQNLHHPRPQHYAAEFQGNTPNFQPPGQAAPGGAYGDFFLDPAASMAAQFAKSSFGLLNGYLQQNFGSYMPVAGELKYYFKVSNAYVWRKLALILFPYRHRDWTRIRSVESGGAASSGPCFAPACEDVNAPDLYIPLMALVSYVLLWALFSGLRGDFHPEVFGFVASQTLACSFVDILIFRVGLYLLNCSAQTSFWDLISFSGYKYVTIIALLCWKQVAGSSWLMYYGVLLAAVGSLSLFLMRSLKFLILPHGLGENPAASISNGQRRLRVQFLFIYSVVVQFLIVLFMCRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8L0</accession>
    <name>A0A1A0H8L0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_21847</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710976.1">
        <property type="nucleotide sequence ID" value="XM_018855110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8L0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8L0"/>
    <dbReference type="GeneID" id="30028086"/>
    <dbReference type="OrthoDB" id="823504at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="177" mass="20388" checksum="C14830FF54BC111B" modified="2016-10-05" version="1" fragment="single">MLDPPERLRKAIVAGNLAISKRLLARFPELWLNVDPAHQGWCNLHYASFHGHYLVCFHLVSQMSRVRLNESSLCDLDLVTFDGLTVLHMPLRHHHSQTLHFLLQEFLGARWLDRRGGPVHRTPLHYCCVFRFIDGVKLLLEYGADWRLPDANGDTCLHLCFAYGGLKCIEALLNYVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Y8</accession>
    <name>A0A1A0H8Y8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_199473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710987.1">
        <property type="nucleotide sequence ID" value="XM_018854914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Y8"/>
    <dbReference type="STRING" id="869754.A0A1A0H8Y8"/>
    <dbReference type="GeneID" id="30027890"/>
    <dbReference type="OrthoDB" id="4087635at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="41856" checksum="89353D774DA60A4D" modified="2016-10-05" version="1">MLSESDDTPAATFRPRFKAKRKPAANKRRLRDMDQDAADTQPAEISVGGASREGVADPRRLSRWARYSALGKLESKAVTGRSLDEDKNGAAADDEDLGSGRVDSGRMESRGTDAMRRDAMNMDTMNMDTTKVGTMNMDTIGGNTMNINSMNMDTTKVGTMNMDTMNMDTMNMDTMNTDSMRMKSMNTGAFNTETMNMDTIKEGTMNMDTIGVSTMNINSMNIDTTNLAPADLATPAAPLSFINSPPSAQNTFVLRHSHFRDTPVLVPLGPEYADRYGNVLDDDVPQVIDPAASDDDIALDAFGQPSPADHAPDLHDMYDLDISVDDVLRRADMPDIPGPAAVLSGLAEKVSLLEMSIRLRRERLLALAASLAELQDARQDALGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H900</accession>
    <name>A0A1A0H900_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_197098</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710880.1">
        <property type="nucleotide sequence ID" value="XM_018854882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H900"/>
    <dbReference type="STRING" id="869754.A0A1A0H900"/>
    <dbReference type="GeneID" id="30027858"/>
    <dbReference type="OrthoDB" id="531329at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="227" mass="26708" checksum="89C264C85A04AB97" modified="2016-10-05" version="1">MSCVYLLLRNLIERNMKHPENAPIRSQQYCRDAHIFFLHWAIMPLPHISQDPLLIVSESSSSIVFPRPRSLLYCFKQSARPPFDNVEAEAELVSGHMTEYSSSIFFYLHMLLFYFFLLQLLFYSTTCIFYKHFRLLVYSTSSSLLSVLATTISYPHYYTNDHLLTVLYQRCTLKNLFGMRLVLYHKQSHVTCTFLRTGSRSTPLLFYKQFLVDTCIVLLLTVIHRMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H985</accession>
    <name>A0A1A0H985_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_83627</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711103.1">
        <property type="nucleotide sequence ID" value="XM_018859233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H985"/>
    <dbReference type="GeneID" id="30032208"/>
    <dbReference type="OrthoDB" id="4085918at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508813" evidence="2">
        <location>
            <begin position="21"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="715" mass="79723" checksum="61449CBE94B660AF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9A5</accession>
    <name>A0A1A0H9A5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_230201</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711123.1">
        <property type="nucleotide sequence ID" value="XM_018855243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9A5"/>
    <dbReference type="STRING" id="869754.A0A1A0H9A5"/>
    <dbReference type="GeneID" id="30028219"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="272" mass="30392" checksum="21971E7F0062D339" modified="2016-10-05" version="1">MSRVGVLVLGPAGAGKSTFCNSIISHMQSIGRRAHIINLDPAAEATEYEFTVDIKDLISVEDVMEEMDLGPNGALVYCFEYLLNNLDWLDEEIGDYNDEYLIFDCPGQIELYTHVPVLPTIVRHLQSSLNFSLCATYLLEAPFVIDRSKFFSGALSAMSAMILLELPHINILSKADLIKSEVSKKQLKRFLNPDPLVLEHDPDNQLNPKYTRLNKAIAGLVDDFGMVQFLPLDCSKDSTSVATILSYIDDVTQWSESQEPKEPAEEVEVDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9A7</accession>
    <name>A0A1A0H9A7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Antiviral helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_32556</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711102.1">
        <property type="nucleotide sequence ID" value="XM_018856082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9A7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9A7"/>
    <dbReference type="GeneID" id="30029058"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000292">
        <property type="term" value="P:RNA fragment catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140746">
        <property type="term" value="P:siRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18024">
        <property type="entry name" value="DEXHc_Mtr4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13154">
        <property type="entry name" value="KOW_Mtr4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000141">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.300:FF:000001">
        <property type="entry name" value="Mtr4 exosome RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.30:FF:000002">
        <property type="entry name" value="superkiller viralicidic activity 2-like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025696">
        <property type="entry name" value="Beta-barrel_MTR4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048392">
        <property type="entry name" value="MTR4-like_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21408">
        <property type="entry name" value="MTR4-like_stalk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13234">
        <property type="entry name" value="MTR4_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="149"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="388"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="29"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="350"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1064" mass="120066" checksum="FF9BAF0D24D428DA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9G4</accession>
    <name>A0A1A0H9G4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Kinesin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32713</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711286.1">
        <property type="nucleotide sequence ID" value="XM_018856115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9G4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9G4"/>
    <dbReference type="GeneID" id="30029091"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903754">
        <property type="term" value="C:cortical microtubule plus-end"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="5">
        <location>
            <begin position="71"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="637"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="152"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="657" mass="72825" checksum="DFE68431E72E8395" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9P6</accession>
    <name>A0A1A0H9P6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_201511</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711129.1">
        <property type="nucleotide sequence ID" value="XM_018854938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9P6"/>
    <dbReference type="GeneID" id="30027914"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="57" mass="6943" checksum="26A53F330DCF0B1C" modified="2016-10-05" version="1">MKQDGPSFDKRYVLRSCSKVFYILLYSFVRLIRRIHQKRKSKNKRERPVVYGLSYTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9S5</accession>
    <name>A0A1A0H9S5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_165330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711389.1">
        <property type="nucleotide sequence ID" value="XM_018854655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9S5"/>
    <dbReference type="GeneID" id="30027631"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="176" mass="19381" checksum="45B5937EAA570893" modified="2016-10-05" version="1">MPATRWSDMVWVPHRTPTRKLPPCTPPPRPNAGERAQGPMLHVYPARRMPHRRFFCRAKLRPDLPQSDLGQPGGKKPTSIPVASNPPSFLPCDACFLSRFLIFCSIACQVPPASHSPLNPAPPHFRNLIPFFFLLATSLPAGTNIFLIRRGLHLDFRPQALCANVGNSATGLPPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9V6</accession>
    <name>A0A1A0H9V6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43248</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711302.1">
        <property type="nucleotide sequence ID" value="XM_018857631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9V6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9V6"/>
    <dbReference type="GeneID" id="30030607"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000219">
        <property type="entry name" value="tRNA (Carboxymethyluridine(34)-5-O)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="58"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="251" mass="28117" checksum="5013FCC35F4057EF" modified="2016-10-05" version="1">MSVPSVPIETQAVPENQEQQFVHEVYNEIAAHFSQTRYKPWPIVEKFLRGQPDYSVGIDVGCGNGKYLGVNPKLVIVGSDRSDGLVTCAQQNSNNAHLLCVADGLHLPHANGRFDFAISIAVIHHFASKARRVDAIRHVLSKVRTRGKFLVYCWALEQEKSRRGYKEGDDQDILVPWVLQKKQKKKPAGAGPGAVPESGDIKTPDLAPETKYRYYHLYREGELVADAETAGGRVVEHGYEKDNWWAIIEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Z2</accession>
    <name>A0A1A0H9Z2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_88212</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711342.1">
        <property type="nucleotide sequence ID" value="XM_018859287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Z2"/>
    <dbReference type="GeneID" id="30032262"/>
    <dbReference type="OrthoDB" id="4024787at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="202" mass="23069" checksum="B6FB014E7D4DE287" modified="2016-10-05" version="1">MDAPQLQSLFSEDSVDLAKTADGFIDHNLIYEQLQERFSRAYSLSQKLFVTEKTQRQALYHYKRRVNALLDVLQDMEGTESADVDQPLEIDHSRLTLLITWRPELASTLNPVLQLAAGTDSPAQIPLKRSYAVNLVVEELIPEIANDELDAVEINPQNTEMWIRRNLLHLIMSKFTPAEVTPKGVREYLDPLPIGSKRKKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAJ8</accession>
    <name>A0A1A0HAJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphatidylinositol N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_40805</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711413.1">
        <property type="nucleotide sequence ID" value="XM_018857316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAJ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HAJ8"/>
    <dbReference type="GeneID" id="30030292"/>
    <dbReference type="OrthoDB" id="196709at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016104">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="32244" checksum="97450611A810A036" modified="2016-10-05" version="1">MFHLPGTISAAHTWRKLLYLRQPFPDNYTHVSFLDQLKRNTTVAQYSYFKLFNDFSLVALYGLLLLLANVNFSAIYSSLWGARIPTVASSAVSLVLLATDASSASGRRHLKLYAVITMLLLIFSPTLRSLTRSSSLDSVWALSFILTCVNVVYHDYGLDPASLYKSVVSTNLSFANAIVLASRLLLSMAAFSYLMFSLEVCVLMPVFDFRLRQHFPRIHYLGMALVCVLVSTMLYFVHGFVFALGYLVSVAFVLFGLPLYFLFLQKYKNELQGPWDTAKPIFRSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAM8</accession>
    <name>A0A1A0HAM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_11741</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711689.1">
        <property type="nucleotide sequence ID" value="XM_018854075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAM8"/>
    <dbReference type="STRING" id="869754.A0A1A0HAM8"/>
    <dbReference type="GeneID" id="30027051"/>
    <dbReference type="OrthoDB" id="5594977at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018814">
        <property type="entry name" value="DUF5427"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28265:SF1">
        <property type="entry name" value="MAINTENANCE OF TELOMERE CAPPING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10310">
        <property type="entry name" value="DUF5427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="167" mass="18821" checksum="E1E0ED0E09039E9E" modified="2016-10-05" version="1">MFSGKAIDGEKLCFANLESSIKHYLQIVDLEKLHIPTEESTDHEINKSNVFISIQPITSGNVDQQSNEEDNMGPAYIEGNNSNSFTFTMILKDITNNITIVSKSQPFPLRWARWVSGRHDDVDSVFHLGDDGESVDPSDWVKDWIQDGLGLTFAVLAQEYVTRRMGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAN4</accession>
    <name>A0A1A0HAN4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_167410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711447.1">
        <property type="nucleotide sequence ID" value="XM_018854672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAN4"/>
    <dbReference type="GeneID" id="30027648"/>
    <dbReference type="OrthoDB" id="4096324at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="215" mass="24889" checksum="4721C696A60F9DDB" modified="2016-10-05" version="1">MNRKRKSTDILAQLGEPLPLPYQANPHTIKSERSFEDSPPVPQPLAYGRIRKRRVSERRELLGLRNEEFNGPNLDEHVPLPIWRRDRGSFSTGSHPETEIEEDIEQSVKTPSVLDELQQQLDLLKSMYDSKDALDADHRKFANVSASLQQIIEQKQQESIVHELERIQKQNKGLRSHQGVNSKWKAGLWLLAFLLLLLASFVAGQFSYEYCYYFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAY2</accession>
    <name>A0A1A0HAY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GET complex, subunit GET2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41549</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711794.1">
        <property type="nucleotide sequence ID" value="XM_018857422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAY2"/>
    <dbReference type="STRING" id="869754.A0A1A0HAY2"/>
    <dbReference type="GeneID" id="30030398"/>
    <dbReference type="OrthoDB" id="4097053at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028143">
        <property type="entry name" value="Get2/sif1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28263:SF1">
        <property type="entry name" value="GOLGI TO ER TRAFFIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08690">
        <property type="entry name" value="GET2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="270"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="42"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="297" mass="31868" checksum="0CA25AA304490961" modified="2016-10-05" version="1">MSELSADEKRRLLRERRQAKMAQGKATERLSNILSQGLSVNSASVQSVLEKTPGSASPDALSDSPNTIRSTQNSASPAFSLGVSHPSLSHDDPEEADMDAIMQKIFSGAGASDARPDGSVPETSQFFAEMMKTMGLDPDALPEMPENASHATQLAVYHEYQQKSVKARFLALRIIVHTANFLYHFLHFSGFAASSHAFVRDVRPQAGSFMSVFISAEISIISGYYLVLSSNGVLSVSSRNHPISKMLSLASAVFPQASALQQLLDSALIYWGGASIVIGDLMLMVVYIGLASILGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB56</accession>
    <name>A0A1A0HB56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_175933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711633.1">
        <property type="nucleotide sequence ID" value="XM_018854723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB56"/>
    <dbReference type="GeneID" id="30027699"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="228" mass="24183" checksum="268CA5636D5F0FFF" modified="2016-10-05" version="1">MIARHSPRTKTARIRRKQKRRQARAGPSPCLQAAASVSTCPSIPDGALSFSANCSADINICSGLGRARHHTVSLYHTGRFTGQCWARQKPGDMCGPTEYRGGKSEPTPGKNMPSSPCAYGARPVACSSEPHAAGLHSARGDKSLASRPGTPRQKHMDTSLAHGKDRYAARPPAAAFLLGTRSSVPPSAGKLRDRVSAYPSTGLPGAPRMRIQDPGDVGRTLEPAINRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBE1</accession>
    <name>A0A1A0HBE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthetase</fullName>
            <ecNumber evidence="7">6.3.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_40974</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>7,8-dihydropteroate + L-glutamate + ATP = 7,8-dihydrofolate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711702.1">
        <property type="nucleotide sequence ID" value="XM_018857336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBE1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBE1"/>
    <dbReference type="GeneID" id="30030312"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="439" mass="48713" checksum="25230523B9B47844" modified="2016-10-05" version="1">MAIELGLKRIRKLLSYLGNPHVSSYKSVHIAGTNGKGSTIAYLSLILTHAKISNGRFTSPHLLSYNDCISINNQTYSLHKFEKVRNSVLSHNARYNLGCTEFEILTATAFKIFEIEQVELALIEVGLGGRLDATNVLTPYANGNTPGGVIVCGITKIGIDHEGFLGNTITDIAREKAGIIKTSVPVVVDHTNQRQALDEIARKAAAVNAPLFSTEKSMACEIDNPIQYSPMLGQYQTENLSVALHIVATIQKTLKSNELTRDCIISGIRNTYWPGRLQRLQDKTTGIEFLLDGAHNECASMELGNYLRSIRRKGFIFVVGMSEGKSVEGLLKHIALKDNDVLIPFHFTAPPEMPWVKCHDVENISMTAKDYVEEVLKMKNADNIADLFDFLAARKNNGDVRQVVICGSLYLCSDILRHQIDLHQEPEQIIELEAATKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBG8</accession>
    <name>A0A1A0HBG8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16 17">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="6 16 17">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_78341</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16 17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16 17">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16 17">Belongs to the CDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6 16 17"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711866.1">
        <property type="nucleotide sequence ID" value="XM_018858867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBG8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBG8"/>
    <dbReference type="GeneID" id="30031843"/>
    <dbReference type="OrthoDB" id="10260889at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070319">
        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006658">
        <property type="term" value="P:phosphatidylserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF8">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 16" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA21356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="428" mass="48060" checksum="E383E2FF73266CC4" modified="2016-10-05" version="1">MEKTASPAARSGTSKTAAPAKTQKDQPPQKNAVVNEKEKKKQAFVTRTIWSLVMLFGFFVLLQSGHFPLILFVVLCQILIFKEIITMTSEPARDKNLPYNKILNWYFLIATVYYLDGESIVGFLNEIHVSSMLVLWYKNHKMVSYSLYLAGFIFFVSTLKKGFYKFQFAQLCASHMTLIIVVFQSHLIIDNILSGIFWFFLPLALVIVNDIFAYLCGITFGRTQLIEISPKKTVEGFLGAWICTGFAAVVISYFLSKSDYLICPATNLNTTIYNYPHCEPNPVFISQLYQLPANIAELVGMDVIFFKPVYFHAAVLATFASLIAPFGGFFASGLKRAFGIKDFGDTIPGHGGMTDRFDCQLLMGSFSYLYLQTFIASSNISSSKLLQMAVFNLTNDQILQLAKALLKYLKTTGTIDPDTLERVFETLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBK7</accession>
    <name>A0A1A0HBK7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_181970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711880.1">
        <property type="nucleotide sequence ID" value="XM_018854777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBK7"/>
    <dbReference type="GeneID" id="30027753"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="363" mass="41818" checksum="6FC92A1C8B2331AB" modified="2016-10-05" version="1">MCPTGAPTASSPGGCIRVPFPGTVASTDFRPPARQAGCRAAQGRLWALASPRHFHRCYQQLVLSCRRRSLPTVHSLHCKSQNPRPRRLRLLPSPGRCPYRSHHRYRCIPEGQHGHLLLGRCHYRSPWQCHFQSPGQCHYHSPGQCHCRSPGQHHPRCFRWLRQCNRPQFQSPRQSRFFPPKRLVDCCMTHLRYLQWKAASLLHQRKPRHLVHCTLRPRWGDMCLSNPRPHRALRWLLPHTWSPLPRFPNYRYPPRCPTQWPAPLDYKPIQARFVIPPPIPPPHYRQYGHFRRFHHGAPHYYSFSSANFAVLAAVGDAIMSAGISLAASKSSRVLVGLSMNHKLGSRKILLLAIKGKCTKKNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBU8</accession>
    <name>A0A1A0HBU8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78432</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711996.1">
        <property type="nucleotide sequence ID" value="XM_018858879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBU8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBU8"/>
    <dbReference type="GeneID" id="30031855"/>
    <dbReference type="OrthoDB" id="198885at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1790.10">
        <property type="entry name" value="Indigoidine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01876">
        <property type="entry name" value="PsiMP_glycosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022830">
        <property type="entry name" value="Indigdn_synthA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007342">
        <property type="entry name" value="PsuG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909:SF1">
        <property type="entry name" value="CARBOHYDRATE KINASE PFKB DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42909">
        <property type="entry name" value="ZGC:136858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04227">
        <property type="entry name" value="Indigoidine_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110581">
        <property type="entry name" value="Indigoidine synthase A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="734" mass="79291" checksum="8318573399042B67" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCY3</accession>
    <name>A0A1A0HCY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Triosephosphate isomerase</fullName>
            <ecNumber evidence="6 11">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_40966</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="6 11"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712252.1">
        <property type="nucleotide sequence ID" value="XM_018857335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCY3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCY3"/>
    <dbReference type="GeneID" id="30030311"/>
    <dbReference type="OrthoDB" id="6715177at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF41">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="248" mass="26659" checksum="0C05F747E2D1EED4" modified="2016-10-05" version="1">MARQFFVGGNFKMNGSKESLSAIVDNLNKADLPQNVEVVIAPPAPYLSLVVEANKQKTVEVSAQNVFAKASGAYTGEISPQQLKDLGANWTLTGHSERRTIIKESDEFIAEKTKFALESGVSVILCIGETLEEKKAGVTLDVCARQLDAVSKIVSDWSNIVLAYEPVWAIGTGLAATPQDAQDIHKEIRAHLAKTIGAEQAEAVRILYGGSVNGKNATEFKDKADVDGFLVGGASLKPEFVDIIKSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD58</accession>
    <name>A0A1A0HD58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_16435</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712328.1">
        <property type="nucleotide sequence ID" value="XM_018854641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD58"/>
    <dbReference type="STRING" id="869754.A0A1A0HD58"/>
    <dbReference type="GeneID" id="30027617"/>
    <dbReference type="OrthoDB" id="4940293at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF8">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="44"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="214" mass="24646" checksum="F419B0321E95A003" modified="2016-10-05" version="1" fragment="single">PPLTSSEKEKQNTRRQRRKMTNDLEAELLEKDDALLTEEQLVIKRKAQNRLAQRAFRERKESKLKELESRLLASEGERQRLLEALDEVRLQFISVKTENQVLRSNASSHTGDMQQSSRFTFPQSQKAFVQQMMDGQEHHVVNADTVSKIYDEPQHPGQKVLAVGAVWDYLLMKIEEEEYENIDMAEVMQALKGNEMCHGYGPAYSLLVVDAALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD68</accession>
    <name>A0A1A0HD68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_40106</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712457.1">
        <property type="nucleotide sequence ID" value="XM_018857230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD68"/>
    <dbReference type="STRING" id="869754.A0A1A0HD68"/>
    <dbReference type="GeneID" id="30030206"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF224">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="117"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="266"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="8">
        <location>
            <begin position="7"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="435" mass="45971" checksum="1B64C49A183B537C" modified="2016-10-05" version="1">MTAANGASRVSSFVSLIKTIVGAGLLSMPLAFSTDGLIGGFFIVLLAALTAGYGLFLQVYVSRYVATGHASFFNVCSISYPALSVVFDLAIAIQCFGCAVSYLVLIGDLMPTIVRGGPGRVFWILASAVLCVPLSFLRNLDSLKYTSIMGLGAIAYLCVLVVLEQILGDLPPEARGEILLLPGGWKSVCSTFSIIVFAFTGHQNMFSIINEAGNKSLVSLATLVNMAVWNAATLFVVVGICGYLTFGNLVGGNVVLLYPDSLGTHVGRFAIVFMVVFLFPLMIHPARLSVNNIYYWVRENGPGTLHSALSSGGHAASGQNSPLLGSAEEPRGPAARNHVVPFSHTTFCVITALLLAAGYYLAVSLSSFALVLAVVGATGSTAISFILPGLFGYKLIGLENERPSRAERMLRGLSLMLTVWGLAVMVVCLYSSLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDJ3</accession>
    <name>A0A1A0HDJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_145303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712541.1">
        <property type="nucleotide sequence ID" value="XM_018854514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDJ3"/>
    <dbReference type="GeneID" id="30027490"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016133">
        <property type="entry name" value="Insect_cyst_antifreeze_prot"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51156">
        <property type="entry name" value="Insect cysteine-rich antifreeze protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="109" mass="13415" checksum="6B41B507DA08D2AD" modified="2016-10-05" version="1">MVLWCYGVLVLWCFGVLVFWCYGAFGLIGVIDVLVFWCYRCYRCYWSYWCYRCYKCYRRYRCCRRYRCYRRYRCYGCYGLIDVMGATDIMGVIGVVMLQVYRCSRCYCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK9</accession>
    <name>A0A1A0HDK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_146432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712597.1">
        <property type="nucleotide sequence ID" value="XM_018854524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDK9"/>
    <dbReference type="GeneID" id="30027500"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="140" mass="15633" checksum="8961B015200CB642" modified="2016-10-05" version="1">MRSGVLRDSSSARSDTSSRASKVREQHCHLIRMKGQGKPAQSRTCGFDHLNPDVLSNARAFICREHPEATPEANSPLYTHQYRPPGHPTGAITLYTHRCHYPVYPPVPSTQTSQYRPPVHPTSAIDPDTFTSAVRYAHTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDP2</accession>
    <name>A0A1A0HDP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712539.1">
        <property type="nucleotide sequence ID" value="XM_018855753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDP2"/>
    <dbReference type="GeneID" id="30028729"/>
    <dbReference type="OrthoDB" id="3997968at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="362" mass="40257" checksum="068DB6FE03C99310" modified="2016-10-05" version="1">MSAAPSTPTRPKALAMGSYTPKSASALRKRLSFERDKFSRASKRSPFLGKEWACDGGLPKPLDSPENSDCSVFSEQNSSATTPITPLSTSKPQFLMPTPLTIGTGRKPRGETGYLRPIAKSRSLLASLQAIRDQPSKEPSEKDASKANGLLEAPEFKNDFGKCLKNSGTYFSPALQLNVSLFSTGQNAPVTSSGIAEMTLFEAKKLLEDLPDRHDANNTPHTPLRKPRIAQNPQFELFVDSDGPVHLMRVEDLDKIPRMKVTNPFIDQDPRDEQTEKQVIDYSTHMEMVNTRTGERKVVVLSEEQRRFKPKKLDFTLAVASPRIPDYKITNKYLGSNIGKNFTMGETGRKGMPDFDIFKDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE23</accession>
    <name>A0A1A0HE23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_39490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712726.1">
        <property type="nucleotide sequence ID" value="XM_018857147.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HE23"/>
    <dbReference type="GeneID" id="30030123"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19672">
        <property type="entry name" value="UBR-box_UBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF24">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="11">
        <location>
            <begin position="102"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="102"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1891" mass="218179" checksum="B14F445C331D8B63" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE36</accession>
    <name>A0A1A0HE36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Carboxypeptidase S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_40675</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712741.1">
        <property type="nucleotide sequence ID" value="XM_018857300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE36"/>
    <dbReference type="STRING" id="869754.A0A1A0HE36"/>
    <dbReference type="GeneID" id="30030276"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017141">
        <property type="entry name" value="Pept_M20_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037217">
        <property type="entry name" value="Carboxypeptidase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="244"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037217-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="540" mass="60541" checksum="24361893FF4EC96A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE60</accession>
    <name>A0A1A0HE60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ELYS-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_148405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712683.1">
        <property type="nucleotide sequence ID" value="XM_018854548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE60"/>
    <dbReference type="GeneID" id="30027524"/>
    <dbReference type="OrthoDB" id="4079278at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27891" checksum="767138BCCB025452" modified="2016-10-05" version="1">MMSNYNYFAIFRIMLAFERECSPRVILKAIRNMTLSQTPYFANLLHDKFQERYNQNPLLARSLIETRKRLIIANLKHGNSNRAAEMWKLNQNSNPEFAQKNVILFQSLIEKLNDEESADLLFNYFPKTLYTDPDIVDFLIAYFGQSPKYRTNFEYITKSLKPPLKRNTLSLLFASFISQDREEAAEKILQVIFKTKNGINSEDFQVIIKKLLAKHQIRQCVDMCLRNDFRVSETHAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE92</accession>
    <name>A0A1A0HE92_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77829</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712925.1">
        <property type="nucleotide sequence ID" value="XM_018858776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE92"/>
    <dbReference type="STRING" id="869754.A0A1A0HE92"/>
    <dbReference type="GeneID" id="30031752"/>
    <dbReference type="OrthoDB" id="2098326at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03046">
        <property type="entry name" value="GST_N_GTT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF9">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00358">
        <property type="entry name" value="Main_(cytGST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="96"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="27601" checksum="53BFEEF877B672EC" modified="2016-10-05" version="1">MTQDRIVLHYLIGSRASRILWLLEELDVEYEMKSYHRTSEGLAPPELKAVWPLGLSPVVQVFRPGAAKPLTLAESGQIVLYLISHYDPQGKFRPESEDDQVLVDYYLHFAEGSLQCHLVSLLVNYVACQRAPWGLGWLVKSIMGKINGFYYLKKIRTAYAFLDQQLEEKNGGYFVGNKLTGADFILDFPVNENLFANPARLEEMGAGVNGERDFPRLAEWNKLITGRLAHVRASEKGQEAKAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE98</accession>
    <name>A0A1A0HE98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Actin binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_148747</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712724.1">
        <property type="nucleotide sequence ID" value="XM_018854552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE98"/>
    <dbReference type="STRING" id="869754.A0A1A0HE98"/>
    <dbReference type="GeneID" id="30027528"/>
    <dbReference type="OrthoDB" id="5971719at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11281">
        <property type="entry name" value="ADF_drebrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829">
        <property type="entry name" value="CORTACTIN AND DREBRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10829:SF25">
        <property type="entry name" value="DREBRIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="ADF-H" evidence="5">
        <location>
            <begin position="5"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="473"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="559"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="308"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="618" mass="68667" checksum="30418A642F569FEB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEL5</accession>
    <name>A0A1A0HEL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712828.1">
        <property type="nucleotide sequence ID" value="XM_018855819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEL5"/>
    <dbReference type="GeneID" id="30028795"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="90" mass="10711" checksum="ACDC99099BDE33C5" modified="2016-10-05" version="1">MTDAVNFFSQVVNVVNSAAQHCFSRFIWLKEQSYITMKNKKPLNSFQLRTKYRNSARLLPLFTALTIEVKNDLKYEMTQFLRRKRSTETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HER3</accession>
    <name>A0A1A0HER3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">BAG domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_77963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713110.1">
        <property type="nucleotide sequence ID" value="XM_018858795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HER3"/>
    <dbReference type="STRING" id="869754.A0A1A0HER3"/>
    <dbReference type="GeneID" id="30031771"/>
    <dbReference type="OrthoDB" id="417450at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.120">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036533">
        <property type="entry name" value="BAG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003103">
        <property type="entry name" value="BAG_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02179">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00264">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63491">
        <property type="entry name" value="BAG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51035">
        <property type="entry name" value="BAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BAG" evidence="2">
        <location>
            <begin position="110"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="202" mass="23421" checksum="A3C62C27985384AC" modified="2016-10-05" version="1">MENLSTHFAQARGSVADTLSQLYARFEHHVPENIEHSVRQYIANISGTTREELFSDIRSLQFTPATVTLALVMMPTIFLAQRLVSSGSSNQAAGKRSKTKKKKPSKAKVANKEIQRILDHVEETYVPQIDSYIEHYAEMSEDDKQYKFKYFEEMLLKELMNLDNVDVVGNDVLRDNRKKVIRFIQDHQRRLDNFKKEAPDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEY1</accession>
    <name>A0A1A0HEY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">valine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_48289</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713160.1">
        <property type="nucleotide sequence ID" value="XM_018858279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEY1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEY1"/>
    <dbReference type="GeneID" id="30031255"/>
    <dbReference type="OrthoDB" id="629407at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000009">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000120">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000005">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="71"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="738"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="986"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1015" mass="114673" checksum="917AEAB19C6D7BD2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF19</accession>
    <name>A0A1A0HF19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED17</name>
        <name evidence="10" type="ORF">METBIDRAFT_38861</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712979.1">
        <property type="nucleotide sequence ID" value="XM_018857059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF19"/>
    <dbReference type="STRING" id="869754.A0A1A0HF19"/>
    <dbReference type="GeneID" id="30030035"/>
    <dbReference type="OrthoDB" id="5319830at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019313">
        <property type="entry name" value="Mediator_Med17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13114:SF7">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10156">
        <property type="entry name" value="Med17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="579" mass="62882" checksum="DB5A128607857285" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF95</accession>
    <name>A0A1A0HF95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_37679</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48096"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11991"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61897"/>
            <dbReference type="EC" id="2.1.1.319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:48097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713136.1">
        <property type="nucleotide sequence ID" value="XM_018856905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF95"/>
    <dbReference type="STRING" id="869754.A0A1A0HF95"/>
    <dbReference type="GeneID" id="30029881"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060567">
        <property type="term" value="P:negative regulation of termination of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000034">
        <property type="entry name" value="Protein arginine N-methyltransferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 10 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="11">
        <location>
            <begin position="55"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="12">
        <location>
            <begin position="158"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="38553" checksum="D4528AEADA024999" modified="2016-10-05" version="1">MSATDKATLPATEQHYFSSYDHFGIHEEMLKDTSRTLLYRLAMFKNKHLFKDKVVLDVGCGTGILSMFAAKAGAKHVYSVDMSSIIDKAKQIVSLNGYADKITLVQGKLEDINLPVDSVDIIVSEWMGYFLLYESMLDTVLYARDKYLVPGGLILPDKCLMYIAGIEDGNYKNDKIHYWEDVYGFDYTPFIEIAMSEPLVDTVENLSLITTPHKFFEFDINTVKKEDLAFHRSFSLKAIDADLCHAYIVWWDCDFPGDEKVTLPTGPMNHYTHWKQTVFYMDQVLDLKKGDLIEGTIDSRPNGHNPRDLDIQIEWGVKTAGQDASRELEGKYNYIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFG7</accession>
    <name>A0A1A0HFG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713104.1">
        <property type="nucleotide sequence ID" value="XM_018853981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFG7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFG7"/>
    <dbReference type="GeneID" id="30026957"/>
    <dbReference type="OrthoDB" id="4070583at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="647"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="219"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="976" mass="106684" checksum="80AEA64275597CA3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFN1</accession>
    <name>A0A1A0HFN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_29264</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713149.1">
        <property type="nucleotide sequence ID" value="XM_018855373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFN1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFN1"/>
    <dbReference type="GeneID" id="30028349"/>
    <dbReference type="OrthoDB" id="1703270at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.168.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.168.20">
        <property type="entry name" value="Ribosomal protein S8e, subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042563">
        <property type="entry name" value="Ribosomal_protein_eS8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="22211" checksum="D915BB7A7CCFE21A" modified="2016-10-05" version="1">MGISRDSRHKRSATGAKRAQFRKKRKFELGRQPAMTKIGGKRIHTVRTRGGNKKFRALRVETGNFSWASEGVARKTRIATVVYHPSNNELVRTNTLTKSAIVQVDATPFKQWFETHYGKVLGKNQTEEPVKKSKKVERKLASRSASANIEDAVEHQFTAGKLYACVSSRPGQSGRCDGYILEGEELAFYLRRLTAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFU8</accession>
    <name>A0A1A0HFU8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713515.1">
        <property type="nucleotide sequence ID" value="XM_018859365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFU8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFU8"/>
    <dbReference type="GeneID" id="30032340"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="38005" checksum="04161A114230FB9E" modified="2016-10-05" version="1">MPYSCTSPTSTTEEDWINEIFEYSPFGNIHEDSFSPLLVPVFSPQKAKESAPEHEDLFSGVSSNNTSATTPGATIHIKDPNHQSHTFFDSVSCKLQRLKESNSLTPKALEWLFNTQDVLADSVDWQTEQKHRNRRSRAFSSQGLEGGPKSSCHLRQKAGSMHSQGLAKRRSSRGKTNKSIFPHGMHSLQTLPYNSRRSSFTNFLTYGSPENEPSSSLSVPQSCCESPFHESWSYTPYFPRSGAGTPGTQSTDFSGLGPSAALFDQENATLLHAEFFPISEAASRSDVSPIAEAPAMYSEYTGPSQQPATENCVLHDTAVQDALAYIDYLQEENQFQQARLEGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG81</accession>
    <name>A0A1A0HG81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial thiamine pyrophosphate carrier 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_76041</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713368.1">
        <property type="nucleotide sequence ID" value="XM_018858466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG81"/>
    <dbReference type="STRING" id="869754.A0A1A0HG81"/>
    <dbReference type="GeneID" id="30031442"/>
    <dbReference type="OrthoDB" id="18574at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090422">
        <property type="term" value="F:thiamine pyrophosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990545">
        <property type="term" value="P:mitochondrial thiamine pyrophosphate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="17"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="116"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="212"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="302" mass="33241" checksum="9DB8967490E4FA9C" modified="2016-10-05" version="1">MQTNPERADHLRKGSSVSPLESLIAGSLSGAVARAVTAPLDTIKIRFQLLLLRDDGVTGIDLVKNLLKKEGFTALWKGNVPAEALYIIYGASQFTTYTILNKCFSDLQNQRKFSLSQLSHLLLVGSGTGMVSTFITYPFDLLRTRLVAHESRVLLSMTDTCKDIYLKNGLKGFFVGIQPTLLSVTASSGLFFWSYSLARDAVDRINNLSPGKIRGVEASCGFIAGVTAKAITFPLDTIRKRMQVSLSGTAHRIFVEHWRSYGFANFYRGFLVSLLKTAPTSAISVAIYEYSISTIRALSSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG82</accession>
    <name>A0A1A0HG82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_9219</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713349.1">
        <property type="nucleotide sequence ID" value="XM_018859336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG82"/>
    <dbReference type="STRING" id="869754.A0A1A0HG82"/>
    <dbReference type="GeneID" id="30032311"/>
    <dbReference type="OrthoDB" id="10030313at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612:SF0">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="6">
        <location>
            <begin position="94"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="5">
        <location>
            <begin position="220"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="483" mass="53242" checksum="6055FF4936DE732D" modified="2016-10-05" version="1">MTEFLDYKVLLQLKDGTTTQGFVTLVNRSQISLGSRSIENSKVKDLKVVQLPGDKLKLRSKASLTQLDDAIVLVSGANSRSATPRLSKKHPDWGSDVQDIKSSEDFDFAANLAMFDKKSVFADFQKHDSVSAQDRLVSHNKVEKRVKNAQDKYDNDEMILSKNKQDNWNKLGSLSQRLSLPVSSGGLPSTGSKVSRDSQVYKFTFEDGSSPVPLASPVQLLEIERIAEESFGIDATISVEICASNLYNMIVSKILGGSVRLSNRKNHNLPPLVLLLIGSGHCSSKAFALGRHLSNHGVRVLAYVINEDIVEADTLRQCQLFEKCGGKVVSTGFPELVDFLNNQLDTPVELVIDSLQGFASQISDLFYTSESMDILREVVRWTNEPGQKFKVLSLDIASGIDGGSGTVSDQLLKINSKFIASLGLPVSGLLHAYNNGSLNDDVQHYVVDLGICNDVYTAKPHLRKFDKFWFCAEGFMKLELISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGA6</accession>
    <name>A0A1A0HGA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Anaphase-promoting complex subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cyclosome subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_62923</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The APC/C complex catalyzes assembly of branched 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on target proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713393.1">
        <property type="nucleotide sequence ID" value="XM_018858328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGA6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGA6"/>
    <dbReference type="GeneID" id="30031304"/>
    <dbReference type="OrthoDB" id="2504561at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037679">
        <property type="entry name" value="Apc5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026000">
        <property type="entry name" value="Apc5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12830:SF9">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12862">
        <property type="entry name" value="ANAPC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 5" evidence="9">
        <location>
            <begin position="306"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="808" mass="92097" checksum="99731FB7EE1DFB87" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGD5</accession>
    <name>A0A1A0HGD5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Clathrin adaptor, mu subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37302</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713423.1">
        <property type="nucleotide sequence ID" value="XM_018856854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGD5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGD5"/>
    <dbReference type="GeneID" id="30029830"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099638">
        <property type="term" value="P:endosome to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09250">
        <property type="entry name" value="AP-1_Mu1_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14835">
        <property type="entry name" value="AP1_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="174"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="443" mass="50958" checksum="BE033DF9DCAD1F33" modified="2016-10-05" version="1">MASQLHFLDIKGKPLLSRDYKGDIPSNTIENFPLILLELESTDDDSLYKPYYNKNGINYVYINHNNLYVCALTRKNENIMTIVIFLSQVIEVLTLYFKSLEEESIRDNFVITYELLDEMMDFGVPQTTDTKILKEYITQDYYKLIKNAPSRLIQPPNALTNAVSWRKDGIVYKKNEAYLDVVESINMLINAKGQVLNSEILGEVKMKSKLSGMPDLRLGLNDKGIFSTNLDDDTASESAPGSKKLEMEDIKFHQCVRLSKFENERIITFIPPDGEFTVMSYRLLSAQFLMKPLILVNCKTVVHKHSRIEINCSVKAQIKKKSTANNVEVIIPLPEDADTPKFVPEYGTIKWIPEKSCLIWKLKTFPGGKQFHMKAELGLPAVTDEENLVPKKPIKVKFSIPYFTTSGIQVRYLRINEPKLQYQSYPWVRYITQSGEDYTVRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS9</accession>
    <name>A0A1A0HGS9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SNF5-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_101896</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713682.1">
        <property type="nucleotide sequence ID" value="XM_018853706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS9"/>
    <dbReference type="GeneID" id="30026682"/>
    <dbReference type="OrthoDB" id="10258327at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006939">
        <property type="entry name" value="SNF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10019">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04855">
        <property type="entry name" value="SNF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="364" mass="41547" checksum="32A7CAD3EB4C83B9" modified="2016-10-05" version="1">MSLAYQSNFYTPQALSTGLTSRLAKDADSALLITNAPQERSAKRNAQQINYAEFDNANDEADFEEEIPGNNAFSIAGVHTTVAAQKRLLKPARLVRLLEDVVGDVAPELAAAARANMGAQQHVLVPIKLNIEFNSGMLKLVDFFMWNASELLVTPDAFAALLCAELELPQHVHLEIVEAITKQLDEYSYVSTLQLPATAEYHVIVDLLVNLDKKLYEDKFEWDLNQTDVTPEDFAEIVVADMGLSREFKPAIAHSLHELTIRLKKEILDGSYNHELHKYQQLTGLIFEKNVRIRTDSSISNGNAVWEPFIEILSPWEIEKREIERERNSRRLKRENMRREVDDYAGSKRRATTRRRLDELDWRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH32</accession>
    <name>A0A1A0HH32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UNC-50 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_36317</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unc-50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713786.1">
        <property type="nucleotide sequence ID" value="XM_018856721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH32"/>
    <dbReference type="STRING" id="869754.A0A1A0HH32"/>
    <dbReference type="GeneID" id="30029697"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007881">
        <property type="entry name" value="UNC-50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12841:SF6">
        <property type="entry name" value="PROTEIN UNC-50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05216">
        <property type="entry name" value="UNC-50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="319" mass="36323" checksum="7CF2E8A0129FBADC" modified="2016-10-05" version="1">MTSRGSLPYTYQDLFPHTATSAREQATPALISAASFTDTLRSGNHHLTPARNLGPEASTWAHWRKLAKRLVRPKTLDFETATWEVFHLIVNPRKMYRSNYTYKHSGKQLYLRDDPSFLILVTGFLVVSAIAWGATYLPSVWDIVKLVVTMVVFDFYVSGAVIATIAWIVSNKLFNPSFSVFTAFLRASRLNINYIDWAFCFDIHCNSFLVIWCLLYLVQFFLLPILTIKDSFLSMLLGNTLYFGAVGHYFVVTFYGFNSLPFVSNSYYTRDASSTTTNPARILQMTILAGVIPVLAIAWLLSVLFHFNVAYTMVHNYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH61</accession>
    <name>A0A1A0HH61_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_80662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713659.1">
        <property type="nucleotide sequence ID" value="XM_018859202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH61"/>
    <dbReference type="GeneID" id="30032178"/>
    <dbReference type="OrthoDB" id="4074938at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="319" mass="36572" checksum="D9E7E89482E6C97B" modified="2016-10-05" version="1">MKISNQRSIVHLDHSGELAFQKKVKVYQTHNVESHILELTADLAALKTQLARVIAKFPFTLKQGESKTIMGKISQKECRDAVVQVMSQVLVLMKKCAVYYQRLLEDLAQFLEEAPKIVAEYADLNRMLGDAYLDFSALPKRCLELIESESCFRARVTFDKMFKCHQYLLGETKRFKKATHDKITSLARVSELTEFSDRLIVLVNLVHDYIYAVSQEDELVCLMRQIYVPGYMCVGHEHLAVKQAYRALVSYYERLRSPAEMLKAVLPVARSLQQVFLMAGRAEDNLSYYYKEAILPLQTGLDAKIRQREADLGEGVVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH62</accession>
    <name>A0A1A0HH62_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_102096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713698.1">
        <property type="nucleotide sequence ID" value="XM_018853712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH62"/>
    <dbReference type="GeneID" id="30026688"/>
    <dbReference type="OrthoDB" id="4077571at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291839" evidence="1">
        <location>
            <begin position="17"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="30872" checksum="B75FD82D73897F72" modified="2016-10-05" version="1">MKFTVLLCFCLTTISANIGPMEVNGRKLYPNLQSFASKEALVDSYLWTHLTHTSDDIIALTTAVFLEDSKTILVLAEPKARDLLSTGKELLGITKKSTINMIEIDLGSNIYSVGSDFFPLVTLSAENSSGQAAVEREVSQVYKIKFVPGVNRIFDLAALSVKAVLGVTLSSQRAEKETITCSANSGGKVQLQVSNRMLYFPQARTRKVKYVSKDKEFIEKEWEAVKSSVADEEHLGALFYEHFKLGKNRCVTNTSYFEDVASRNWLEWSEPYRLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHK5</accession>
    <name>A0A1A0HHK5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714125.1">
        <property type="nucleotide sequence ID" value="XM_018855549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHK5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHK5"/>
    <dbReference type="GeneID" id="30028525"/>
    <dbReference type="OrthoDB" id="5595141at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043189">
        <property type="term" value="C:H4/H2A histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012423">
        <property type="entry name" value="Eaf7/MRGBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07904">
        <property type="entry name" value="Eaf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="34265" checksum="69F7B7FEC356BBE6" modified="2016-10-05" version="1">MPGKRIWSIEQEILLLSLVCDSKPAGAERDNNIAKILVNINENITGEDSFTEEQIWTKLADFFDLQKIEDIEKDSEGEPGTPRKEERSRSSAGAKSKDEPTRESTVEQRGTPDAAGPKPETNSPAKSAEPPELYSSELSDVEGEETELEKLHDKNLTLPPPREIKRRGRPKKAVKRLPAKENASKESLATTPKKRMRPTTKDGEDESAPKRRPLSSQGTRRKAKTDTTPEAEQASNQPSQAEDSAGRLRDDTRRENTGSGDEVYPKDEEPANAETKSEIPQDSEIADEGPKTRRSRRQAVRRSTRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHL4</accession>
    <name>A0A1A0HHL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_110119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714135.1">
        <property type="nucleotide sequence ID" value="XM_018853901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHL4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHL4"/>
    <dbReference type="GeneID" id="30026877"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF15">
        <property type="entry name" value="HSP90 CO-CHAPERONE HCH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="146" mass="17121" checksum="4050F75565F1F54D" modified="2016-10-05" version="1">MVVHNPNNWHWVDKNCLPWAKLYMDNNVKDTTFEDNTFKFVLKSVDSVLGDCDVTQRKGKVLCIYDMKLLFSIEGKKKDEEKDLLGTITIDEFVHDQDEDEYFFGVTSDHSLDIKRFFLPVLRTKLMKFQLDLILAHGRDVQDTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHV3</accession>
    <name>A0A1A0HHV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Saccharopine dehydrogenase [NAD(+), L-lysine-forming]</fullName>
            <shortName evidence="13">SDH</shortName>
            <ecNumber evidence="4 13">1.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">Lysine--2-oxoglutarate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_9693</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-saccharopine + NAD(+) + H2O = L-lysine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="EC" id="1.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AlaDH/PNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.7" evidence="4 13"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713904.1">
        <property type="nucleotide sequence ID" value="XM_018859439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHV3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHV3"/>
    <dbReference type="GeneID" id="30032414"/>
    <dbReference type="OrthoDB" id="265306at2759"/>
    <dbReference type="UniPathway" id="UPA00033">
        <property type="reaction ID" value="UER00034"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004754">
        <property type="term" value="F:saccharopine dehydrogenase (NAD+, L-lysine-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12188">
        <property type="entry name" value="SDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000217">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000423">
        <property type="entry name" value="Saccharopine dehydrogenase [NAD(+), L-lysine-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007886">
        <property type="entry name" value="AlaDH/PNT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007698">
        <property type="entry name" value="AlaDH/PNT_NAD(H)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027281">
        <property type="entry name" value="Lys1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF23">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE [NAD(+), L-LYSINE-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05222">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018250">
        <property type="entry name" value="Saccharopine_DH_Lys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01002">
        <property type="entry name" value="AlaDh_PNT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01003">
        <property type="entry name" value="AlaDh_PNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="8 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase N-terminal" evidence="18">
        <location>
            <begin position="5"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Alanine dehydrogenase/pyridine nucleotide transhydrogenase NAD(H)-binding" evidence="17">
        <location>
            <begin position="181"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="317"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="204"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR018250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR018250-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA23423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="373" mass="40984" checksum="46E8AF2CEF3AD283" modified="2016-10-05" version="1">MVTLHLRAETKPLEARAALTPTTTKQLLDAGYEVYVEESEQSIFKAEEYAAVGATIVPAGSWKTAPADRLIIGLKELPENETFPLVHEHIQFAHCYKDQAGWQEVLQRFPAGKGTLYDLEFLENEQGRRVAAFGFYAGFAGAAIGVLDWAFKQTHSDSEDLPAVSPYPNESALVAHVKKELDAALQKNGGQQPTCLVIGALGRCGSGALDLFRKIGIPEENLLKWDMKETAKGGPFEEIVQSDIFINCIYLSTPIPPFIDFASLNKPDRKLRSIVDVSADTTNPHNPIPVYTIATVFNDPTVPVETSAGPKLSVCSIDHLPSLLPREASEFFSKDLMPSLLQLKDRQTAPVWARAKELFDHHVARLPAADGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI37</accession>
    <name>A0A1A0HI37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_9766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713989.1">
        <property type="nucleotide sequence ID" value="XM_018859458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI37"/>
    <dbReference type="GeneID" id="30032433"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="303" mass="33977" checksum="043AF6023C7363E6" modified="2016-10-05" version="1">MRREEKLSEYTALKASTESIMDFNLEKNDPRDHSKVTSHFLSEQSIDSECLSPSSFSGGHNFCFHSEFEWMKNQASTSHHSTGSCEYLDSRVRPSSALGHETSEGYSQLQKGKKEKIENTDLSDMCQNTPKRFSMELVNKENIYNKKRGSFAVVASSPIRSNKRIANNDRVSSPTVENVGRENSESLIHKIQSNEDPKEPFRKVFTNLSSRACGHTSSSYRKISHTDIAWKDAEGLSGRKTQIPHVTKKKGFVRSLLSSPGLALSRIKYHSVGTQKTSTSCLKLTSESSRGSGLELGPDMYFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI85</accession>
    <name>A0A1A0HI85_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein S16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_30131</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714192.1">
        <property type="nucleotide sequence ID" value="XM_018855564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI85"/>
    <dbReference type="STRING" id="869754.A0A1A0HI85"/>
    <dbReference type="GeneID" id="30028540"/>
    <dbReference type="OrthoDB" id="407221at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1320.10:FF:000013">
        <property type="entry name" value="Mitochondrial ribosomal protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_bS16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_bS16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020592">
        <property type="entry name" value="Ribosomal_bS16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_bS16_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919:SF20">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="Ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00732">
        <property type="entry name" value="RIBOSOMAL_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="135" mass="15661" checksum="B191D1E4E12427F9" modified="2016-10-05" version="1">MSVKSTPIKIRLARFGRKHQPVYNIVVARIRSARNSLPIEVLGTYNPITVPLTPDEKARNVQPYKHIELDFHRTKYWLGVGALTSDRVAFLLKRAGLLPELWPKENKLTQVISKRVSQEPTIETEEKKDFFRPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIA9</accession>
    <name>A0A1A0HIA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36515</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714217.1">
        <property type="nucleotide sequence ID" value="XM_018856749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIA9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIA9"/>
    <dbReference type="GeneID" id="30029725"/>
    <dbReference type="OrthoDB" id="4070448at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013665">
        <property type="entry name" value="Sfi1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08457">
        <property type="entry name" value="Sfi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sfi1 spindle body" evidence="2">
        <location>
            <begin position="590"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1068"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1158"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1172"/>
            <end position="1184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1232" mass="143013" checksum="A9AF5F24FBB209A8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH7</accession>
    <name>A0A1A0HIH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Arginine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_35687</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714124.1">
        <property type="nucleotide sequence ID" value="XM_018856644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH7"/>
    <dbReference type="GeneID" id="30029620"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF4">
        <property type="entry name" value="ARGININE PERMEASE CAN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="374"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="446"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="42"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="60967" checksum="113E10850183901D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIK0</accession>
    <name>A0A1A0HIK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_114121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714321.1">
        <property type="nucleotide sequence ID" value="XM_018853978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIK0"/>
    <dbReference type="GeneID" id="30026954"/>
    <dbReference type="OrthoDB" id="4088889at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035189">
        <property type="entry name" value="Std1/Mth1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17235">
        <property type="entry name" value="STD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="390" mass="43415" checksum="F2F4BF2B68DA73BD" modified="2016-10-05" version="1">MGLLDFLALGTGAASENIPTFRRGSGRDAVDLQVRSLLYLFKRLRVNRLASFSLGETLKYTDGKLWDQLLISHEAEIKKLDKAYNLSGFQKVLVEDEHRVLIDWPKNKFRALCILSEYPAMSLNSSNAAFEENALPVFLDYKERSTHSFAFSILPVKTSTSTRDLALDLAQSLLYYEHHSYVSLMSRLQTAEKAIAKFCGADIKMVELSEATKADIVHEYLKQLAFLVQLVRIYQQYVNPKDSETPLSSPKKAAVADQPRALRPAKSFLQLPNAASSPLRPSLSPKKSMASLSGSPKKLMGDISSGNPDVSPRKQLQARPSISSFRANEIYNPVTSPPISCTNSENKRMGSGVLLSHLSESGRETTELWEKCKASIREKLAKEKEQLGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIR1</accession>
    <name>A0A1A0HIR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="7">2.3.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37965</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + acetyl-CoA = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:17017"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="2.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="7">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.12" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714372.1">
        <property type="nucleotide sequence ID" value="XM_018856934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIR1"/>
    <dbReference type="STRING" id="869754.A0A1A0HIR1"/>
    <dbReference type="GeneID" id="30029910"/>
    <dbReference type="OrthoDB" id="537444at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000003">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006257">
        <property type="entry name" value="LAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01349">
        <property type="entry name" value="PDHac_trf_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF90">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 7" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="45"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="10">
        <location>
            <begin position="185"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="131"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="137"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="148"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00065810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="472" mass="49702" checksum="F0C77F7A7CD85E80" modified="2016-10-05" version="1">MSALFARTAAVSVRAMAAQNSRHYLASSFSLQMARLYSSGNFPSHVVIKMPALSPTMTQGGIASWSKAVGDELTPGEPIAEIETDKASMDFEFQEEGYLAKILVDAGAQDVPVGKPIAVYVEDKADVGAFDGFTASDAGEGEAPAAAPKEDEPKKQEPAETPAAASEKPASPAKAPAKAPAGRILASPLAKTIALDKGIALKNIKGSGPNGRIVAKDVENYKAPEAAPAAAAAAAAPAATFQDIPITGMRKTIASRLLQSTQQSPSYIVQSHINVSKLMKLRQSLNSTAEDRYRLSVNDLLIKAIALASLRVPEANSAWMADQGIIRQYNVVDVSVAVATPAGLITPIVKNAHTKGLAAISKEMKALGKKAKDNKLSPEEYQGGTICISNLGMNNAVNAFTSIINPPQSAIVAIGTVEKKAVPSSVNEQGFVFEDFITITGTFDHRTVDGAKGGEWIRELKKIVENPLELMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIY5</accession>
    <name>A0A1A0HIY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_10289</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.</text>
            <dbReference type="EC" id="3.2.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714600.1">
        <property type="nucleotide sequence ID" value="XM_018853730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIY5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIY5"/>
    <dbReference type="GeneID" id="30026706"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="entry name" value="Glycoside hydrolase, family 3, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050288">
        <property type="entry name" value="Cellulose_deg_GH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011658">
        <property type="entry name" value="PA14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715">
        <property type="entry name" value="BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42715:SF27">
        <property type="entry name" value="BETA-GLUCOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07691">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56988">
        <property type="entry name" value="Anthrax protective antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 3 N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="7">
        <location>
            <begin position="204"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type III-like" evidence="8">
        <location>
            <begin position="393"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="479" mass="53565" checksum="7E7A48186A486202" modified="2016-10-05" version="1">MTAYNRVNGEHLSQSKKLLQKVLRQDWKWNGMVMSNWYGVYSMKESTDAGLYLEMPGPTRFRELVQTSHVVVWNEIHMEIIDGNVRHVLQFVNDCLGAKISKAQAESKSTDPAVSQLLINNAGESIVLLENEDNILPLSAEKTPGNEAIALLGPNFKAKRNSTGGPACFSARYTITPTKRPKGSECHIPCKCPGAQHRGLIEERSVATTKIVLSEYRTEKLPAGQQLFYVDFEGYYIPEETVEYQFGCSCLGTEQMFLDGELIVDNKTSKVKGDAFVMGMGTRKSAQKLSWEEAGRGGTRVFWNRGQVHRAGSFGRGRDGPKKADKVVLVTGLSKEHESEGYYEKIGTHPSYAFGHGFSYTTFELGVPDIILGEDDVRVSVKVTNTGKVAGDEVVHFYVELTSPNIIRPKKELKDFAKSKWALGESKVVSVKLPTLDVTSYWNSYKNKGHLQSDCGYVFQQDHRPCTLTLSDYWDWDVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJC1</accession>
    <name>A0A1A0HJC1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34459</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714577.1">
        <property type="nucleotide sequence ID" value="XM_018856476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJC1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJC1"/>
    <dbReference type="GeneID" id="30029452"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF81">
        <property type="entry name" value="OS08G0157400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="287"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="10"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="230"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="434"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="477"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
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        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="237"/>
            <end position="251"/>
        </location>
    </feature>
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        <location>
            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
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        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="438"/>
            <end position="453"/>
        </location>
    </feature>
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        <location>
            <begin position="477"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="499"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="531" mass="57607" checksum="FE9002BDC486C7B9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJJ1</accession>
    <name>A0A1A0HJJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Topoisomerase I damage affected protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_10330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018853741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJJ1"/>
    <dbReference type="GeneID" id="30026717"/>
    <dbReference type="OrthoDB" id="4036304at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291943" description="Topoisomerase I damage affected protein 11" evidence="3">
        <location>
            <begin position="29"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="293"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="482"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="531"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="557" mass="58876" checksum="72E6084B659910AB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJS0</accession>
    <name>A0A1A0HJS0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Beta-glucan synthesis-associated</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_121488</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714727.1">
        <property type="nucleotide sequence ID" value="XM_018854177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJS0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJS0"/>
    <dbReference type="GeneID" id="30027153"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000140">
        <property type="entry name" value="Beta-glucan synthesis-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF1">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="256"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="128"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="661" mass="73839" checksum="F04D6730DD388B24" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK07</accession>
    <name>A0A1A0HK07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance rate of mitochondria protein 29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_77153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0538 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714817.1">
        <property type="nucleotide sequence ID" value="XM_018858653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK07"/>
    <dbReference type="GeneID" id="30031629"/>
    <dbReference type="OrthoDB" id="937at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018794">
        <property type="entry name" value="UPF0538"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444">
        <property type="entry name" value="UPF0538 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18444:SF9">
        <property type="entry name" value="UPF0538 PROTEIN C2ORF76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10209">
        <property type="entry name" value="DUF2340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17257" checksum="699FE2BBC0A32A30" modified="2016-10-05" version="1">MSEFNIEEPLTSNVRPASDALVTIRIIKSFPYRNVKNHVMTSINLKTTTPSQLLESIQKIIDTTGSLRPYRNVKYDTVKVYTHAHGSKSMNLVINFENDEKDILHCEDKTNLVQKEQCLWDLGVENETEISIFNYQDYAAYKANPEEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK12</accession>
    <name>A0A1A0HK12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribosomal protein L15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_36890</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714822.1">
        <property type="nucleotide sequence ID" value="XM_018856794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK12"/>
    <dbReference type="STRING" id="869754.A0A1A0HK12"/>
    <dbReference type="GeneID" id="30029770"/>
    <dbReference type="OrthoDB" id="361383at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="99"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="43"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="281" mass="30755" checksum="27C91A598F57A167" modified="2016-10-05" version="1">MLRFFTGAKSLFSGITPAVQATRNASYLGNLKPAEGAVKAYTRLGRGPASGKGKTAGRGQKGQKARGSVPHWLEGGQTPYYKLFPIVGFKRPHRKIYQEISLHRIQGFWNAGRIPLEAGGTLTIRAMRECGLLSGSLKDGVKILGTGDSFYNVPLNVEACQGTTRAIAAVRKTGHEFTSVYFTKLGLLAHVFPERFLLKKGRVPLQARPLHKRDIAYYSDPARGGYLLKDRSLLLDHVGKPNLRKAAVEKSALEALLATALTKTHTDYAESKIVRLADLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK18</accession>
    <name>A0A1A0HK18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome P450</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77305</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715005.1">
        <property type="nucleotide sequence ID" value="XM_018858674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK18"/>
    <dbReference type="STRING" id="869754.A0A1A0HK18"/>
    <dbReference type="GeneID" id="30031650"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050364">
        <property type="entry name" value="Cytochrome_P450_fung"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46300">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="524" mass="59954" checksum="71B5829FD2584FD8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK27</accession>
    <name>A0A1A0HK27_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">UEV-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_62688</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715015.1">
        <property type="nucleotide sequence ID" value="XM_018858327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK27"/>
    <dbReference type="STRING" id="869754.A0A1A0HK27"/>
    <dbReference type="GeneID" id="30031303"/>
    <dbReference type="OrthoDB" id="306304at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072666">
        <property type="term" value="P:establishment of protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd11685">
        <property type="entry name" value="UEV_TSG101-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052070">
        <property type="entry name" value="ESCRT-I_UEV_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017916">
        <property type="entry name" value="SB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008883">
        <property type="entry name" value="UEV_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306:SF3">
        <property type="entry name" value="TUMOR SUPPRESSOR PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23306">
        <property type="entry name" value="TUMOR SUSCEPTIBILITY GENE 101 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05743">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09454">
        <property type="entry name" value="Vps23_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51322">
        <property type="entry name" value="UEV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="UEV" evidence="8">
        <location>
            <begin position="7"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="490" mass="55077" checksum="EB7B3CCB0E5CECF9" modified="2016-10-05" version="1">MSKVSKKITNWLYSVLQPQYTHPEIAYKDIYQFLQVYIDQGFRIRTAVYTSVHGGSELLVNLYGPLICGNNVFQVSIWIPLNYPLADTSGKASGYDANGVPITFAVPSKGQIIRPGNNVDSQGKVYHPFLVSWYNSTSTRQMMDQFNLLLLMECLKSTFERSSPIGLAPQTTGPTLPPKSPSKINSPQQARISTLSSGPPLPEKFAHSGNSSELIPQKYRGPPPLPTYTTQNQWVGIDSSQSQSAMIQSPGQSTLTRYLNTLDRATTVKGEVSRPRPSVETNQGETSVIEDLMDKVTLESKKENSDPRELQRIAQHINRYLNPGNPESVNNKIPRVRKTRNIAKALYSQLLHHKNQARANAENLDKHVQYLEDQVQTVRYSNQKLNDLTELNKKSSTEVVLSASENRKIALDDLVLPDLMLVRQLYDSVAEIRGYKDAIRLVGGTFKSEPELINNLTLESCIRSVRALSRELFWLEVTRDEIGRLMSLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK73</accession>
    <name>A0A1A0HK73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Regulator of phospholipase D SRF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_16545</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714768.1">
        <property type="nucleotide sequence ID" value="XM_018854658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK73"/>
    <dbReference type="GeneID" id="30027634"/>
    <dbReference type="OrthoDB" id="2589563at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037737">
        <property type="entry name" value="Srf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36819">
        <property type="entry name" value="REGULATOR OF PHOSPHOLIPASE D SRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36819:SF1">
        <property type="entry name" value="REGULATOR OF PHOSPHOLIPASE D SRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39868" checksum="D879C0D94A2371B3" modified="2016-10-05" version="1" fragment="single">NPFAQTQLLTYSLYPKTVPNYVLDKLSLAHYKEQLKNSNVNNDQAEISPAVKAHSTKQDDDKEYASYMHDPFIKSLNNNWANLLVAVKQPTEVPTDTVSKHESLKANPDWETPWGGDERLRSALLGSNDHAASTSDAHVPMWRRMLSRKKPLVADDSPRLRTKAGYWMLDEKRKDLMPTLWRIFVQNPFLPLLLRITILMFSACALALAVSIFVYLNRQYEGTSIEQQPSTIFAIVVQSFAIVYIVYIAYDEYSGKPLGLRNPLGKMKLIMLDLLFIMCSSANMSLTFDSLYDEKWVCKPDNSSTDSGLYPTVNFLCRRQRALASFLLLVLALWVLTFTISIIRVVDRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK91</accession>
    <name>A0A1A0HK91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucose-signaling factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714902.1">
        <property type="nucleotide sequence ID" value="XM_018856883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK91"/>
    <dbReference type="STRING" id="869754.A0A1A0HK91"/>
    <dbReference type="GeneID" id="30029859"/>
    <dbReference type="OrthoDB" id="4076669at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022757">
        <property type="entry name" value="Gsf2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11055">
        <property type="entry name" value="Gsf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="363" mass="41613" checksum="E966A96A839CE185" modified="2016-10-05" version="1">MSPKEILPESSLEIYLRFNDDMEKDYCLQITSETVFRDLFKVFQTLPISLRPNLFYDPQPVLFVVLTAPGYLTEDGALLFSYETGQEKYQKRVALDDVVAKQCWPGQLVLPVWRFNHFGYYMFISALVVWLYTDLPDFVSPTPGICLTNQMSYLLSWAAQKYNFNHIADVFIKDLQEPVNIGAQCAFFIFHIVKVLVVFFLVWSGMFNPTRLLRLGPQKKPVVTKETLIALGWTGSKRANADEYKDEYREYKIKEFGGMVPAHQASVFTKLKHLGVFLGDHEGFNTPVNPANKLSDMSDDKFVLSYDYFVKQGEFFEEFTAGKDAEQINEAIKQFRRYGLLHSGGVIADLVQKRKAAGDSKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKB9</accession>
    <name>A0A1A0HKB9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nop14-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_36587</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714729.1">
        <property type="nucleotide sequence ID" value="XM_018856762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKB9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKB9"/>
    <dbReference type="GeneID" id="30029738"/>
    <dbReference type="OrthoDB" id="441771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007276">
        <property type="entry name" value="Nop14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183">
        <property type="entry name" value="NOP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23183:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04147">
        <property type="entry name" value="Nop14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="286"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="354"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="793"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="286"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="327"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="366"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="797"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="814" mass="92313" checksum="09B5730D17CBF2F8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKL1</accession>
    <name>A0A1A0HKL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_77340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715046.1">
        <property type="nucleotide sequence ID" value="XM_018858679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKL1"/>
    <dbReference type="GeneID" id="30031655"/>
    <dbReference type="OrthoDB" id="4090559at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="220" mass="25051" checksum="356C46C7646F68B3" modified="2016-10-05" version="1">MSTYLDMPLLISDHDGEDSEGESSNGVKNSSADEVESVNPALSATQEQSALSPESFNCERNKVMKIVVKYVAMKITNSFPPQSSRNVDPTEMPLDEYLMILVSRLQLSLPLFMKGIIYLFRYMDIIYLLRYVNQSNNFANYTDMGYGLKKLIIGCYRLVLARERRSNDWSKITGLSNAQINDIVKTVVGRLNGKLLIKNVELIRLRSEIFRFVKMVTKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKT5</accession>
    <name>A0A1A0HKT5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endonuclease/exonuclease/phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_10558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714899.1">
        <property type="nucleotide sequence ID" value="XM_018853799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKT5"/>
    <dbReference type="GeneID" id="30026775"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20801" checksum="E574ECB1DC5A11E1" modified="2016-10-05" version="1">MKQALVLIKKTENFSEYIRFQTEPRFLFYTFICGDFNSRQFDPVFSPAKLPCPLKEQYGKAASKVPYEAFMETRNDLINSHQRVYDKLISLYSIAYDLADRGISLTEIWSFIDYIFVLQDNKISQSPGIDALEEFAKVSNIKVLALLRVPKKSEIGKSRLSQPAVGTCPSDHVCLMADLELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKW0</accession>
    <name>A0A1A0HKW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_38221</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714924.1">
        <property type="nucleotide sequence ID" value="XM_018856972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKW0"/>
    <dbReference type="STRING" id="869754.A0A1A0HKW0"/>
    <dbReference type="GeneID" id="30029948"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071256">
        <property type="term" value="C:translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046967">
        <property type="term" value="P:cytosol to endoplasmic reticulum transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="112"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="596"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="644"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="603"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="664" mass="74429" checksum="F69DF68A66644D28" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HL73</accession>
    <name>A0A1A0HL73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GroES-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_10696</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715044.1">
        <property type="nucleotide sequence ID" value="XM_018853828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HL73"/>
    <dbReference type="STRING" id="869754.A0A1A0HL73"/>
    <dbReference type="GeneID" id="30026804"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08254">
        <property type="entry name" value="hydroxyacyl_CoA_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF8">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="7">
        <location>
            <begin position="15"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="38460" checksum="992C247120C162F3" modified="2016-10-05" version="1">MITPRTQTGYGFKRGSKEIVKFENLPVPEPSANGVLLKIEAAGLCRSDDHILISQNPGSPDVMVMGHEICGTIAQAGSAIVDNLRYKVGARFCMFISDSCGSCDNCREGRDNHCSVNTYQAFGITKDGGFQEYLLVENPRNLIPIPDSVSFKEAASATDAILTPFHAIMKVKSMLSPTSKVLVLGAGGLGLHAIQILRHFDCKIICVDQKPENEDLVKSLGAHEFYRSFQDVDYKQESFDVSFDFIGNQNSVDCSCKYIGSNGKIVMIGMGKLRVMLPNYDLSRREVEVIYNFGGTSREQGEVLKWIAAGKIKPIVETQPMDQLPDYMRKMRRGEIVGRVAFKPVSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GWK1</accession>
    <name>A0A1A0GWK1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_137696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000016">
        <property type="protein sequence ID" value="OBA16121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709232.1">
        <property type="nucleotide sequence ID" value="XM_018854424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GWK1"/>
    <dbReference type="GeneID" id="30027400"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA16121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="24384" checksum="13BD9D0E2B65B814" modified="2016-10-05" version="1">MYPLSSGASDASSLSPASSMYPPSSGASKPSSLSSSSVHSSSSASLGPKYTTTYTTTETDHSIVTKTIVVCVTTDATGCVTTVTSTVLHCPTTDCEPTELPNPTITGETPVPTASCNEGGSCTPAVETTSNIAPVPSGAAKVSYTTETLIITTTISKGPASYATSTLTVTRTVSIDCDTCETGEGSNPTVAPATDAPGHVLGSTTSSGTMTATLISSISPFEGGAGSRSVVLGMFVLSLLAFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ63</accession>
    <name>A0A1A0GZ63_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">LUC7-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_48059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA16987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709284.1">
        <property type="nucleotide sequence ID" value="XM_018858254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ63"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ63"/>
    <dbReference type="GeneID" id="30031230"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA16987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="236" mass="27200" checksum="D34A729F77D2B362" modified="2016-10-05" version="1">MADDQRKLLEQLMGKEALDSFQVKRKEVEMTNPRVCKAFLVGTCPHDLFAGTKLNIGRCPSLHLEKHKLEYEYRTKKKNDKFPSFEHEYYKLLQKYIDEIDFTIATALKRLEHTPEEKAKIAAVTKDLDVLDTKIGLMTSEIRYLAAEGETQKAFEQSARLEEVLKQRDALADQARLIAENIGQSAQQKLQVCEVCGAYLSRLDSDRRLADHFIGKIHLGYAQMRADFDELKKRYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1X0</accession>
    <name>A0A1A0H1X0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2 6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_61742</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family. FKBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2 6"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709424.1">
        <property type="nucleotide sequence ID" value="XM_018858319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1X0"/>
    <dbReference type="STRING" id="869754.A0A1A0H1X0"/>
    <dbReference type="GeneID" id="30031295"/>
    <dbReference type="OrthoDB" id="1902587at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005528">
        <property type="term" value="F:FK506 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000006">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044609">
        <property type="entry name" value="FKBP2/11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45779:SF7">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291856" description="peptidylprolyl isomerase" evidence="7">
        <location>
            <begin position="24"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="8">
        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA18029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="138" mass="14928" checksum="C184C63753225777" modified="2016-10-05" version="1">MLYGFTSKAIATIAIANAVLASALQIDVTKSVEKCEYRAKNGDEVSVLYTGKLADGTVFDTTDSRGRPIVFKLGTGRVIKGWDQGILDMCVGEQRTLYIPSELGYGLRNVGPIPANSDLIFDVELVDVSGKSRHTDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H217</accession>
    <name>A0A1A0H217_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_61599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709362.1">
        <property type="nucleotide sequence ID" value="XM_018858318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H217"/>
    <dbReference type="STRING" id="869754.A0A1A0H217"/>
    <dbReference type="GeneID" id="30031294"/>
    <dbReference type="OrthoDB" id="5239630at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019034">
        <property type="entry name" value="UPF0390"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09495">
        <property type="entry name" value="DUF2462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="85" mass="9433" checksum="A259F373AEE4A637" modified="2016-10-05" version="1" fragment="single">MAQGNLKLKSKGKGRVTKRQSNLRAAAPVIIKPKKAVAKQAYNLRKSVGSLNGTEKLISSRVGHLEIIKGSRRQIEREQKEAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Y7</accession>
    <name>A0A1A0H4Y7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_74980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709523.1">
        <property type="nucleotide sequence ID" value="XM_018858426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Y7"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Y7"/>
    <dbReference type="GeneID" id="30031402"/>
    <dbReference type="OrthoDB" id="2018507at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000147">
        <property type="term" value="P:actin cortical patch assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22967:SF65">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE AKL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="15"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="362"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="669"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="372"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="688"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA18988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="720" mass="81710" checksum="D40C45C97E522F51" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H530</accession>
    <name>A0A1A0H530_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709716.1">
        <property type="nucleotide sequence ID" value="XM_018856281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H530"/>
    <dbReference type="GeneID" id="30029257"/>
    <dbReference type="OrthoDB" id="3981230at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="413" mass="45185" checksum="B17269599E9453BF" modified="2016-10-05" version="1">MDSSFDVSFNASALLEDHQLDTSVLLLADDGLYAGSPAKKHVPPPEALCQSLSGTTHPELPCLFLLLPPTPALQGPLALVSGELFQMTHHLRQERNLHNNKIHKLLADLQGDAGDFALDAQLRTRVFKARVSRQNSLGLLDTPLDPLDVQLDAYLRDPAARPRPRSGSPSKRKGLASAGFATANENKENAAPVLEPSKRKLSDSARKPLKKSRSYASIPALQTAHTNALAKGDLKDNLSKLGLKDNLPKVDIPDILLGLLDNLVPGLRDVRFSPRRTCVPRGAALPGRPSLKMRDDLRIFLVDLLTGSVADATQFGTELNASNCEGFPLPDNANEVVQIPTNDTVPASAAQKMAIIRALVGPALRPRPESDVPKYKGFYSKREYDDQRVRASPEVTIFHDAKKRVRWADELEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H572</accession>
    <name>A0A1A0H572_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Potassium channel tetramerisation-type BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_13807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709592.1">
        <property type="nucleotide sequence ID" value="XM_018854430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H572"/>
    <dbReference type="STRING" id="869754.A0A1A0H572"/>
    <dbReference type="GeneID" id="30027406"/>
    <dbReference type="OrthoDB" id="2414723at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758">
        <property type="entry name" value="BTB/POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31758:SF2">
        <property type="entry name" value="BTB_POZ DOMAIN-CONTAINING PROTEIN YLR108C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="424" mass="49027" checksum="EC6A3B90AE46C81A" modified="2016-10-05" version="1">MSLELLDQVDPKIPEFLPHDMVYSIQVGYKLFQVSGASLLSDAPSYFTRFFDQPGNAGKTLFIDRSPLVFKKVYMHLQGYHISVDTDYEFVHLWQDAYYFCLTRLQKLLRDEPLIARVGDTLFKIPRVFLYGPGNHPNFFLIGMEGLSTKSTILNSDVSLIRPPPQRPTLTINRSPILFADLLEIFRGNYEILRNDEHRTNLIKECRYYRFLQLEQAIIKHKIIRNPFTQKEDIILNLVNLNKAGIINLSTSLEDEQPISYIRPYLRKEPRRHLIIQIDSDVDFDAKLIIPKTGDLSFLVLTNRFATKMSQVFELLAKTFSNDGGKTKLVLPCNLLDAAVTVNGIELKKSWFREFFGPLEEVPEDEPPHKRKRCDTGMKGEVVDFKLKQSLWRITSRTDKLLLQAVSINAVSDQFNFNKSVEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B5</accession>
    <name>A0A1A0H5B5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Actin-related protein 2/3 complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_13529</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the WD repeat ARPC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709758.1">
        <property type="nucleotide sequence ID" value="XM_018854384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5B5"/>
    <dbReference type="GeneID" id="30027360"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000601">
        <property type="term" value="P:positive regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017383">
        <property type="entry name" value="ARPC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10709:SF2">
        <property type="entry name" value="ACTIN-RELATED PROTEIN 2_3 COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038093">
        <property type="entry name" value="ARP2/3_su1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="53"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="9">
        <location>
            <begin position="110"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="381" mass="41671" checksum="85A6F2FDA4CEE50E" modified="2016-10-05" version="1">MSPPLVYQLGHKPIKDHCFSPDFSLLAVTKDNTVELYQTAPNSPNKPQLVATLKGHDKTVTSVDISPDGSKILTCSQDRNALVWEQTDSTGEYKPTLVLLRINRAATVCKWSPNGDKFAVGSADRVIAVCYYEEENDWWVSKHLKKPLKSTITSLSWHPNNVLLASGSTDGHVRVFSAYIKGVDSKPEPSVWGSKLPFQTLVGDYTNATGSWIHDVAFNGDGVGDSLAYVSHDGTISVVYPQGEGLPPSSFVTVKTSYLPFKSMLFLGDSIIVGGHNCNLIVFQGDESGWKEVFVVEKQKDLIHDVSPSTDDDAEISSHQALNMFKEMDLKGRVNKPHLQGSGRALSTTHQNTISSLRWYDGGKISTSGIDGKIVIFSLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5I1</accession>
    <name>A0A1A0H5I1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PhoD-like phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709881.1">
        <property type="nucleotide sequence ID" value="XM_018856321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5I1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5I1"/>
    <dbReference type="GeneID" id="30029297"/>
    <dbReference type="OrthoDB" id="2419400at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043904">
        <property type="entry name" value="PhoD_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46689:SF1">
        <property type="entry name" value="PHOD-LIKE PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19050">
        <property type="entry name" value="PhoD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase" evidence="1">
        <location>
            <begin position="137"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD-like phosphatase" evidence="1">
        <location>
            <begin position="429"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="688" mass="77781" checksum="A5103FBD8C36FB15" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5K6</accession>
    <name>A0A1A0H5K6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709762.1">
        <property type="nucleotide sequence ID" value="XM_018854385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5K6"/>
    <dbReference type="GeneID" id="30027361"/>
    <dbReference type="OrthoDB" id="4093586at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="303" mass="32305" checksum="60D6EB3DA2B328BF" modified="2016-10-05" version="1">MTRLDSKWADHGGPAPTAPKQDAKTKTLGDGRLIAKPAAQKLPSKWADAPDPEPVHRAPPNNNTQLPTPPSSAGNGAKGASRERGHRGGRRRSEGKPDRSSSENSGESRGSRANGHSKEVRQERRKSLSDSGRSLASRLGSLQVSDERSGARDGKPRKDHAGRGASHGRHPKAPARGSGGESDDEGPGGKMSAAALSFALRLGVPEKERKASGQTPQPGRDKRPAPSAPGPGASRRDQPAPAPAGSRYLTPKQKRALEEHERQARLAKERELKDAQLKAEVQSIFEKMGDKTTSWADLEDESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5X1</accession>
    <name>A0A1A0H5X1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">endopeptidase La</fullName>
            <ecNumber evidence="7">3.4.21.53</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_45897</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Hydrolysis of proteins in presence of ATP.</text>
            <dbReference type="EC" id="3.4.21.53"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.53" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709959.1">
        <property type="nucleotide sequence ID" value="XM_018857973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5X1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5X1"/>
    <dbReference type="GeneID" id="30030949"/>
    <dbReference type="OrthoDB" id="2411602at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19500">
        <property type="entry name" value="RecA-like_Lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000021">
        <property type="entry name" value="Lon protease homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004815">
        <property type="entry name" value="Lon_bac/euk-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054594">
        <property type="entry name" value="Lon_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003111">
        <property type="entry name" value="Lon_prtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00763">
        <property type="entry name" value="lon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718">
        <property type="entry name" value="LON PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43718:SF2">
        <property type="entry name" value="LON PROTEASE HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22667">
        <property type="entry name" value="Lon_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02190">
        <property type="entry name" value="LON_substr_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00464">
        <property type="entry name" value="LON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51787">
        <property type="entry name" value="LON_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 8 12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Lon N-terminal" evidence="11">
        <location>
            <begin position="196"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="10">
        <location>
            <begin position="872"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="782"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="122"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="966"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1086" mass="118618" checksum="7E374F7ACD576EFD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Y4</accession>
    <name>A0A1A0H5Y4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_79840</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709849.1">
        <property type="nucleotide sequence ID" value="XM_018859136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Y4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Y4"/>
    <dbReference type="GeneID" id="30032112"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF4">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="400" mass="44409" checksum="FD0270312A346717" modified="2016-10-05" version="1">MLAFKKSFSVLLRHRADFSHVVIGGGVVGVAVGSELQLIPSNNVAVLEKHSMLGMETTSRNSEVIHAGIYYPPTSLKARLCVRGKELIYRKLDPKIVPYRQCGKWVVAQSENEGKYLEKLHQNASDMSVSTKFVTADKVKKQHPLIRAEYGALDSPTTGIISSHDLTQYFQAQFENAEGTLAINTRVCGLEYRSQVLDYLVHCVEMGSGEKFTVSTDNIINCAGLHAPKIANMLLPSSRHFRSLFAKGSYFSYVPETPVSTSLITTRLIYPCPHPNASGLGTHMTFDMGGQIRFGPDLEWIKESIEDASTLDYSVSLINIEAAQKAIASYYPSIKISEIQPSYSGFRPKLYSQSESVEKFSDFYIKEEKGFPGFVNMIGIESPGLTSAFAIAEYVREIYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H664</accession>
    <name>A0A1A0H664_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ran-binding-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710107.1">
        <property type="nucleotide sequence ID" value="XM_018858026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H664"/>
    <dbReference type="GeneID" id="30031002"/>
    <dbReference type="OrthoDB" id="512915at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008812">
        <property type="entry name" value="Ran_GTP-bd-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010:SF2">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31010">
        <property type="entry name" value="RAN-SPECIFIC GTPASE-ACTIVATING PROTEIN 30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05508">
        <property type="entry name" value="Ran-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="432" mass="47332" checksum="E4775E2C5A3B7073" modified="2016-10-05" version="1">MDEILAKASSQAVTFAIRSGISLASGFAIKKVSQLLDKIPPSEKARLEKTSARLNTKTAILATSIDLIKLSAARGNSALEATVDMVSDLKDEIDTFDAEIAAILQGFSSANEKESVRAAERKISALLDSVNDAIPLINLALITSGVSLNNAMDPRVSPSRLLQAASHVTGSNAEYARRSEGPVSVGPVFDLKLYSVFYNPSRLKYVDETSAASTEGGASAVSWKEEFARALCLIERVPGKLYEYDLVVTEDFDDGRYHDDNEAPVTRRYKVRDISKQFFSASGKLLRLEGSNSPVLTLKIQTAGAPEYVALGEVGTSFDDADSDNDDADGDPDTALINAKELQTNEKFRSLSLLEYLVRLVSLQESEQMSILEVKDEKLLLYLHDRTDKSFVPLSHEEKRRLQTKSESHGHMLQVDSSVHRLKNLSLQEDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H689</accession>
    <name>A0A1A0H689_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_214240</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710003.1">
        <property type="nucleotide sequence ID" value="XM_018855072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H689"/>
    <dbReference type="STRING" id="869754.A0A1A0H689"/>
    <dbReference type="GeneID" id="30028048"/>
    <dbReference type="OrthoDB" id="1112565at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08368">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09397">
        <property type="entry name" value="LIM1_UF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0440">LIM domain</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="5">
        <location>
            <begin position="767"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="48"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="384"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="475"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="611"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="200"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="528"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="849" mass="92726" checksum="BB7434572544B88D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6G6</accession>
    <name>A0A1A0H6G6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710083.1">
        <property type="nucleotide sequence ID" value="XM_018858020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6G6"/>
    <dbReference type="GeneID" id="30030996"/>
    <dbReference type="OrthoDB" id="5398685at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="64" mass="7291" checksum="BFC1DA62DE24E52A" modified="2016-10-05" version="1">MTQENVSLAEAYESIANAEKQASLLEKMLDNLDSKMDSILDEIENNRTEIKETEKKQADTDKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6J5</accession>
    <name>A0A1A0H6J5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ribonuclease T2-like</fullName>
            <ecNumber evidence="4">4.6.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_59876</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Rnase which modulates cell survival under stress conditions. Released from the vacuole to the cytoplasm during stress to promote tRNA and rRNA cleavage and to activate separately a downstream pathway that promotes cell death. Involved in cell size, vacuolar morphology and growth at high temperatures and high salt concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the RNase T2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.19" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710061.1">
        <property type="nucleotide sequence ID" value="XM_018858309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6J5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6J5"/>
    <dbReference type="GeneID" id="30031285"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000004">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057328">
        <property type="entry name" value="RNaseT2L_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25488">
        <property type="entry name" value="RNaseT2L_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291723" description="Ribonuclease T2-like" evidence="18">
        <location>
            <begin position="17"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="RNase T2-like C-terminal" evidence="19">
        <location>
            <begin position="316"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="active site" evidence="16">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF25488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA19533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="428" mass="47900" checksum="76767ABDAA85581A" modified="2016-10-05" version="1">MKVSIFTLLALVQAAALQSSETEKKNSRFFPAKFWPDTAKLGPEQEIFGGGQSSLLCPVNLPLTCSNSTPIYDSCCFESPGGIMLQTQFWDYYPAIGRNDSFTLHGLWPDNCDGSYEQFCDGKLNIERGDIRRIVVDEYKDEALFSKIEESWKNFNGDDELLWVHEFNKHATCIKTIRPECYGAEFTRDRNIYDFLLIAVNLYEKYPTFQFLKDKGIVPSLTDTYTYDEIAEALSSNFNGHNVYFKCNRYQALQEIWYFHYLQGPLTNEEFVQIPSMLHSNCPKTGIKFLPKGDFRPPPGQPPKSPGGKAGTIKLSGHSGCLISNGQWYTQGTCAKFQAKDLQFGGTNLVSSKGVCGVTEAGVLNCNRQNQASKFQFQVDKSSGVVSYGGKSEWCLHEAGKHGSGKFAQVPIKLADGNCETFQITLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6W1</accession>
    <name>A0A1A0H6W1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710218.1">
        <property type="nucleotide sequence ID" value="XM_018854359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6W1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6W1"/>
    <dbReference type="GeneID" id="30027335"/>
    <dbReference type="OrthoDB" id="3980110at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="920"/>
            <end position="931"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="944"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="944" mass="104999" checksum="2E79DE96E3FED5EB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6X6</accession>
    <name>A0A1A0H6X6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_79658</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710190.1">
        <property type="nucleotide sequence ID" value="XM_018859107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6X6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6X6"/>
    <dbReference type="GeneID" id="30032083"/>
    <dbReference type="OrthoDB" id="2015333at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04043">
        <property type="entry name" value="C2_Munc13_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052811">
        <property type="entry name" value="Glucose_resp_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010439">
        <property type="entry name" value="MUN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014770">
        <property type="entry name" value="Munc13_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014772">
        <property type="entry name" value="Munc13_dom-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263">
        <property type="entry name" value="ADENYLATE CYCLASE ACTIVATION PROTEIN GIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47263:SF1">
        <property type="entry name" value="C2 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_7G02350)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06292">
        <property type="entry name" value="MUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51258">
        <property type="entry name" value="MHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51259">
        <property type="entry name" value="MHD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD1" evidence="2">
        <location>
            <begin position="492"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="1">
        <location>
            <begin position="691"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="domain" description="MHD2" evidence="3">
        <location>
            <begin position="894"/>
            <end position="1050"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1170" mass="131412" checksum="98CA3CD57E1B9D58" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H747</accession>
    <name>A0A1A0H747_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein 60, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_45644</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710315.1">
        <property type="nucleotide sequence ID" value="XM_018857940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H747"/>
    <dbReference type="STRING" id="869754.A0A1A0H747"/>
    <dbReference type="GeneID" id="30030916"/>
    <dbReference type="OrthoDB" id="1733909at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045041">
        <property type="term" value="P:protein import into mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="567" mass="60052" checksum="286643D393BF7A2A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H3</accession>
    <name>A0A1A0H7H3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_44421</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710501.1">
        <property type="nucleotide sequence ID" value="XM_018857791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H3"/>
    <dbReference type="GeneID" id="30030767"/>
    <dbReference type="OrthoDB" id="2264563at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF15">
        <property type="entry name" value="ACTIVATOR OF SKN7 PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="445"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="615"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="102"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="771" mass="86752" checksum="62B0429B468F0AD3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H805</accession>
    <name>A0A1A0H805_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Acetolactate synthase</fullName>
            <ecNumber evidence="4 12">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_13053</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="12">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 12"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710553.1">
        <property type="nucleotide sequence ID" value="XM_018854296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H805"/>
    <dbReference type="STRING" id="869754.A0A1A0H805"/>
    <dbReference type="GeneID" id="30027272"/>
    <dbReference type="OrthoDB" id="16262at2759"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901705">
        <property type="term" value="P:L-isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000008">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000007">
        <property type="entry name" value="Acetolactate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000053">
        <property type="entry name" value="Acetolactate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="16">
        <location>
            <begin position="81"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="14">
        <location>
            <begin position="277"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="15">
        <location>
            <begin position="484"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA20028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="675" mass="73481" checksum="921B2A02DF5B84A4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H815</accession>
    <name>A0A1A0H815_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Activator of Hsp90 ATPase AHSA1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_44970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710563.1">
        <property type="nucleotide sequence ID" value="XM_018857857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H815"/>
    <dbReference type="STRING" id="869754.A0A1A0H815"/>
    <dbReference type="GeneID" id="30030833"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08892">
        <property type="entry name" value="SRPBCC_Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.20">
        <property type="entry name" value="Activator of Hsp90 ATPase Aha1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036338">
        <property type="entry name" value="Aha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015310">
        <property type="entry name" value="AHSA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="ASHA1/2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 HSP90 CO-CHAPERONE AHA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13009:SF22">
        <property type="entry name" value="LD43819P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09229">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01000">
        <property type="entry name" value="Aha1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103111">
        <property type="entry name" value="Activator of Hsp90 ATPase, Aha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Activator of Hsp90 ATPase AHSA1-like N-terminal" evidence="3">
        <location>
            <begin position="13"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="37540" checksum="D965B00F19893271" modified="2016-10-05" version="1">MVVNNPNNWHWVDKNCIDWSKAYFNSNLIGLSAKTDDNRDVQVKAISSLEGDVEVCQRKGKVISIFDLRIVLNIEGSSAQDKDFKGSITVPELAYDTEEDELQFDISIYNEDSDSEKMRIVIKKELVPQIKSVLMNFGGDLIKEHGSDIQHESDKVHSKLTKENQSALQAKEAKDKEQAQKPAPVPSARPVTETSSSSVPKYNTSTLHLEPTFNTTAEQLYITLLDPARVSAWSRSNAQIQAKEGSEFLLFGGSISGKILELVTNERIRQAWRLDSWRTGHFAELDIQLKQGAGETTMVVKWSGIPIGDEDRVKGNFQDYYVRAIKLTFGFGAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H816</accession>
    <name>A0A1A0H816_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Clavaminate synthase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33102</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710691.1">
        <property type="nucleotide sequence ID" value="XM_018856222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H816"/>
    <dbReference type="STRING" id="869754.A0A1A0H816"/>
    <dbReference type="GeneID" id="30029198"/>
    <dbReference type="OrthoDB" id="406634at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF25">
        <property type="entry name" value="OXIDOREDUCTASE AIM17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD/TfdA-like" evidence="7">
        <location>
            <begin position="174"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="52347" checksum="598F1AA7D3A2DCB6" modified="2016-10-05" version="1">MGLKNIFLRSKPTFLIQSCNVYQKRSLSLVSFDDRRITVSFPEDVGTGDKAEEDLEADHTPQTQVTFSTIFLRDACESPESVDVSTRQKSFTTAQVAKNLKVHGEPSIVRKNNQDHLIVQWKHDDGSTQKSSFSEQVLKKYKDFPSRMAGKFFMEDKIYWSEKELVKDMKLLNLDYHAFLEGGSTFSKALKALNKHGICFVNKMDDPLQNPKTQDLCEANLALWPVAKVAKNFGYIKPTFYGTLFDVKNDKNAKNIANTNKFLPLHMDLCYYESPPGLQLLHFIKNSTLGGENVFADSFLAVSKIRKEDSEAYEALKKVPITFHYKNNNEYYYYLRPLIVEDPHLVDNKSGQPLVKEVNYSPPFQGPFEVNITSTEDPKLFDDFIRGMIMFEDAINDRANQYIVKTPENSCVIFDNRRVLHSRLEFSDCNGGDRWLMGCYVDGDSFRSRLRTHMSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H820</accession>
    <name>A0A1A0H820_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710568.1">
        <property type="nucleotide sequence ID" value="XM_018856196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H820"/>
    <dbReference type="GeneID" id="30029172"/>
    <dbReference type="OrthoDB" id="4085151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="38047" checksum="224048FAF7B04A74" modified="2016-10-05" version="1">MSYRFIFDIDDFESEVRQWAANRDMSCDENHASRQNLKFDPRVWNGLAWKHSVLAMQLTVVLLVAYMRLFRSVHESIESLYEHRAQSRITECIGEATLGLATSNKKGKKVSFLLPEPRLFSIEYLEKKVHVVGHLILPSKRQIQSAFVCLGLGTFLLPRALHHAFHFHFICAKWLKFTSDNLIASILLFTGFELASIASLKLSSPTSLCNIRVAFSRFIQFYGVFLLLISMLALVQANDTLKLYVYKMLAPCGSANRWSSFFDVRVPNENILSAILGETMMKYTNGKLSENLSPQQAHEVIQGAIDLFARGLGDCVTVLLFATLVFVPGALVHVCGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H867</accession>
    <name>A0A1A0H867_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_79387</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710613.1">
        <property type="nucleotide sequence ID" value="XM_018859055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H867"/>
    <dbReference type="STRING" id="869754.A0A1A0H867"/>
    <dbReference type="GeneID" id="30032031"/>
    <dbReference type="OrthoDB" id="409586at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF51">
        <property type="entry name" value="CARRIER PROTEIN YMC2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="14"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="109"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="209"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="297" mass="32566" checksum="22E39DD7A6DC19EA" modified="2016-10-05" version="1">MIEIEEPDFRQSDNQVFKDLFGGTMGGIAQVLVGQPFDTVKVRLQSAPEGTYNGAVDVIKKLMAKEGLGGFYKGTLTPLIGVGACVSVQFSVNEFMKRYYEEKLQGQPLSLLQFFNCGAVAGFANGFLTSPIEHIRIRLQTQTTKSFSGPIDCFKKIYSVGGLASGIYKGLAPTLFRESVGLGIYFATYEALISRELGNKIYATRTDIPGWKLCMFGGLSGYTLWLSIYPVDVIKSKLQTDSLNKPTYKGSVDVIRDISTKVGVRGFYKGFVPTILRAAPANGATFAVFELMMRLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8B0</accession>
    <name>A0A1A0H8B0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Metal resistance protein YCF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_44026</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710783.1">
        <property type="nucleotide sequence ID" value="XM_018857736.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H8B0"/>
    <dbReference type="GeneID" id="30030712"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015431">
        <property type="term" value="F:ABC-type glutathione S-conjugate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18595">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000450">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000001">
        <property type="entry name" value="ATP-binding cassette subfamily C member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="511"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="934"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="977"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1063"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="1161"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="277"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="604"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="16">
        <location>
            <begin position="943"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="1257"/>
            <end position="1502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA20261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1514" mass="169011" checksum="4B88E3BE87E8BBB3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8E5</accession>
    <name>A0A1A0H8E5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translationally-controlled tumor protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_12299</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in protein synthesis. Involved in microtubule stabilization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TCTP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710777.1">
        <property type="nucleotide sequence ID" value="XM_018854206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8E5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8E5"/>
    <dbReference type="GeneID" id="30027182"/>
    <dbReference type="OrthoDB" id="10248936at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.150.10:FF:000002">
        <property type="entry name" value="Translationally-controlled tumor protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018103">
        <property type="entry name" value="Translation_control_tumour_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01002">
        <property type="entry name" value="TCTP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01003">
        <property type="entry name" value="TCTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="5">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="168" mass="18721" checksum="ED5208BBC388A984" modified="2016-10-05" version="1">MKIYTDIFSGDELLSDAYDLKEVDNVVYEADCAMVQVSSGGDVDIGANASAEEAGDDLEEGVETVNNVVYSFRLSQTSFDKKSFLTYIKGYMKAVKAKLAETDPDQVEVFEKGAQAYVKKVIGSFKDWDFYTGESMDPDAMIVLLNYREDGTTPYVVIWKHGVKETKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8E9</accession>
    <name>A0A1A0H8E9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_43429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710911.1">
        <property type="nucleotide sequence ID" value="XM_018857654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8E9"/>
    <dbReference type="GeneID" id="30030630"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291684" evidence="1">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="66" mass="7369" checksum="325973FC9091D2AA" modified="2016-10-05" version="1" fragment="single">KKIAAPQFRIWSPTTLLSVALWSLTNVDRTGNSVFSMVWPQPRNLGRCNYITAKQKSPSPSAHSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8K6</accession>
    <name>A0A1A0H8K6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_83517</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710971.1">
        <property type="nucleotide sequence ID" value="XM_018859229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8K6"/>
    <dbReference type="STRING" id="869754.A0A1A0H8K6"/>
    <dbReference type="GeneID" id="30032204"/>
    <dbReference type="OrthoDB" id="432483at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097035">
        <property type="term" value="P:regulation of membrane lipid distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000749">
        <property type="term" value="P:response to pheromone triggering conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05574">
        <property type="entry name" value="STKc_phototropin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000121">
        <property type="entry name" value="Serine/threonine-protein kinase nrc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45637">
        <property type="entry name" value="FLIPPASE KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="430"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="110"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="331"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="732"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="122"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="738"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="818" mass="89878" checksum="99ADB56D7551BD53" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U9</accession>
    <name>A0A1A0H8U9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710958.1">
        <property type="nucleotide sequence ID" value="XM_018857769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U9"/>
    <dbReference type="STRING" id="869754.A0A1A0H8U9"/>
    <dbReference type="GeneID" id="30030745"/>
    <dbReference type="OrthoDB" id="18110at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524:SF1">
        <property type="entry name" value="MRH DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23524">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="119"/>
        </location>
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        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="231"/>
        </location>
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            <end position="271"/>
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            <end position="341"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="385"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="465"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="498"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="524" mass="55674" checksum="C7F1D9D7025C5FA1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8W1</accession>
    <name>A0A1A0H8W1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_23304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711081.1">
        <property type="nucleotide sequence ID" value="XM_018855260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8W1"/>
    <dbReference type="GeneID" id="30028236"/>
    <dbReference type="OrthoDB" id="412109at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="434" mass="48738" checksum="288E85C8DC85D02B" modified="2016-10-05" version="1" fragment="single">SSTPIHVHNGTSSLFNSPLKKLDRLDLNNIKHRRASQSSEHDSQSDSDSNDEIANATVVEAEDSEGDGDHDPPSDPLQCLDPPVYVRKRKRADHTAMTPKFSFIKELHTPAEDMSGIMDNETNESYLKISFSASDSTPCPPQPRKKFRRNLQDGTPSQPSKISGPILNLMSSKKFSISGAPLVNKLDGAEKSEEDTSFKSSPASTTSLCKTKLSSTPISQSTPMNSRPGSPSQAVHGVGEKHVWCSLPRPDLKYSYYTPQPKLTTTANRITSETKDFVRNYHSNNILMPFENYKIVGDFPISAAGLMDEGDQNIHIADKRINDPYLVIPHGMKRKKFDDILMQYLNTSNRLPLLKHFESDLGIDEMKLLINDGKSVRYFYEEVLSLSDYDTRDFLKRERIRWHPDKWGSRLADSCFDMSLIGSLSLVINVLLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8W5</accession>
    <name>A0A1A0H8W5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710973.1">
        <property type="nucleotide sequence ID" value="XM_018856050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8W5"/>
    <dbReference type="GeneID" id="30029026"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="9770" checksum="CA20D55BF699CEE8" modified="2016-10-05" version="1">MHCVQFIDKWTKLYNAIFGVWTDLTSVSYQYSWAGPAVEKLSFTNSLALTPQINKLSGTLSIYRVLWGITICLFAMVIQLFSSLMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8X5</accession>
    <name>A0A1A0H8X5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_12358</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710855.1">
        <property type="nucleotide sequence ID" value="XM_018854215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8X5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8X5"/>
    <dbReference type="GeneID" id="30027191"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="10">
        <location>
            <begin position="206"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="103"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="401" mass="43309" checksum="24D625F4276A0AD9" modified="2016-10-05" version="1">MAAKNHSSTPARLAVRELAQDADDSSLKIIRMYHNKRYLPHSERVSNLAWRIQNNKALKQARGRLLKPARAPAPPGRPDPTAASKAPDDFDYVAHIRRISQDEYAAQARGPPARSASFQLGATTTPSPAMAPADPLFLDSQWPPPPPDPPASSFLSSYIDSLESTIKNDYHPVRPRDQSLAAADSSGHYVSDKVPLDRRQARLLACSNCHTRTTPLWRKTAAGDTLCNACGLFYKLHGTLRPANAAFAPPPDARGPRPAPPNPLFQGSVDGAISSRNTGLFSQMGDVPHFTNPSQHVSAGVSTQAVARLKPGDFFGSAELMAMLPAAWGSNAPDAADNGADEIDRLLNMNLFQLDHLALDKLGSGFAGHGFDSRGMEATDEILIDEAATGLANYNWLDFQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H913</accession>
    <name>A0A1A0H913_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CoaE-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43539</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711136.1">
        <property type="nucleotide sequence ID" value="XM_018857672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H913"/>
    <dbReference type="STRING" id="869754.A0A1A0H913"/>
    <dbReference type="GeneID" id="30030648"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990143">
        <property type="term" value="C:CoA-synthesizing protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031315">
        <property type="term" value="C:extrinsic component of mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00152">
        <property type="entry name" value="dephospho-CoA kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695:SF46">
        <property type="entry name" value="BIFUNCTIONAL COENZYME A SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10695">
        <property type="entry name" value="DEPHOSPHO-COA KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="243" mass="26946" checksum="842925550A1E0B42" modified="2016-10-05" version="1">MLVIGLTGGIATGKSTVSKNLLIQGIPVVDADTIAREVVEPGRRAHKQVVEAFDLQVPDLLDEGGRLNRAALGQAVFGNSERLKTLNKIVHGAVKREIARQLFWTYVSGASMAVLDVPLLFEAGLDHICGVTISVSTDDTTQLKRLLERNPELSEEDAKKRILSQLPMEVRDSKADKVIDNNGSLEELHEKTIAIMEDLRPCRLWTILEWFPPFGIFVAVTTVTIRALKSSIRAREQRKEKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H922</accession>
    <name>A0A1A0H922_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribosomal protein L30p/L7e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_42948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711028.1">
        <property type="nucleotide sequence ID" value="XM_018857600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H922"/>
    <dbReference type="STRING" id="869754.A0A1A0H922"/>
    <dbReference type="GeneID" id="30030576"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000465">
        <property type="term" value="P:exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF26">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="268" mass="29944" checksum="94D7B8AB1B0AE454" modified="2016-10-05" version="1">MAVLNSNPEIVLRKRKDADRKRVEKQDAARVRMEKKGRKVAVPGEKFLRAEVLASRATANKIEGKRLENILKHEQAKAAISAQKETPAKLAFIIRTEKQNKAVSIPTKALNVFKVLRLDEPNMGVFVKLTPTIEPVLKLISPFIVVGTPSLASVRHLFQKRACIPSSEDDQSPSKLDNNQAVEDKFGDDLGFICIEDLIHEITSLGDNFKAVTLWLAPFSLTAPVNGWSPLAKLAKMRYQEENKKATTLAGHAKLEEIDIDKFIEEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H945</accession>
    <name>A0A1A0H945_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_32413</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710924.1">
        <property type="nucleotide sequence ID" value="XM_018856043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H945"/>
    <dbReference type="STRING" id="869754.A0A1A0H945"/>
    <dbReference type="GeneID" id="30029019"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="11">
        <location>
            <begin position="37"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="398"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="439"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="521" mass="58276" checksum="F45422EBCC7B7D13" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9A0</accession>
    <name>A0A1A0H9A0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_12577</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 18">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="14"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711118.1">
        <property type="nucleotide sequence ID" value="XM_018854237.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H9A0"/>
    <dbReference type="GeneID" id="30027213"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02736">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02583">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.274.100:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.180:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035698">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035697">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015700">
        <property type="entry name" value="RPC1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14 16" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="253"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1459" mass="162553" checksum="4B1941448F251DF9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9A8</accession>
    <name>A0A1A0H9A8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_42960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710994.1">
        <property type="nucleotide sequence ID" value="XM_018857601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9A8"/>
    <dbReference type="STRING" id="869754.A0A1A0H9A8"/>
    <dbReference type="GeneID" id="30030577"/>
    <dbReference type="OrthoDB" id="2238745at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF174">
        <property type="entry name" value="ARRESTIN-RELATED TRAFFICKING ADAPTER 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="240"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="513" mass="56788" checksum="BE7A87902EE772A5" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9B7</accession>
    <name>A0A1A0H9B7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_201116</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711112.1">
        <property type="nucleotide sequence ID" value="XM_018854936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9B7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9B7"/>
    <dbReference type="GeneID" id="30027912"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000003">
        <property type="entry name" value="Proteasome subunit beta type-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="22677" checksum="CFF56164CB7C3D38" modified="2016-10-05" version="1">MSDPSSINGGSAVAMVGKECIAIACDLRLGNQSLGLANNFEKVFQFGDKTFLGLTGLASDVLTLKEDFRLKHNLYKLREEREIEPKTLANLVSSSLYEKRFGPYFIGPIVAGLESKTNKPFICGFDLIGCIDFAKDFIVSGTASDQLYGMCESLYEPDLEPEDLFETISQALLNAVDRDALSGWGAVVYVVTKDKVVKRVLKTRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9K4</accession>
    <name>A0A1A0H9K4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_71833</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ACBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711192.1">
        <property type="nucleotide sequence ID" value="XM_018858398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9K4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9K4"/>
    <dbReference type="GeneID" id="30031374"/>
    <dbReference type="OrthoDB" id="346910at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036042">
        <property type="term" value="F:long-chain fatty acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000010">
        <property type="entry name" value="Acyl-CoA-binding domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022408">
        <property type="entry name" value="Acyl-CoA-binding_prot_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310:SF62">
        <property type="entry name" value="ACYL-COA BINDING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23310">
        <property type="entry name" value="ACYL-COA-BINDING PROTEIN, ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00880">
        <property type="entry name" value="ACB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="9609" checksum="B07FDCA7DBF761E4" modified="2016-10-05" version="1">MVSTEFTAKAEAVNALTKRPSDDELLSLYGLYKQATVGDNTTAKPGMFDLKGKYKWQAWEDLKGTSQADAEAQYIELVNKLLAQYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9X4</accession>
    <name>A0A1A0H9X4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_202763</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711195.1">
        <property type="nucleotide sequence ID" value="XM_018854954.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H9X4"/>
    <dbReference type="GeneID" id="30027930"/>
    <dbReference type="OrthoDB" id="162894at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000340">
        <property type="entry name" value="Serine threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001008">
        <property type="entry name" value="Serine/threonine-protein kinase cek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE GREATWALL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="14">
        <location>
            <begin position="56"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="808"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="1237"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="13">
        <location>
            <begin position="1514"/>
            <end position="1628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="378"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="578"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="734"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1057"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1276"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1354"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1398"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1474"/>
            <end position="1502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="599"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="740"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="766"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1064"/>
            <end position="1077"/>
        </location>
    </feature>
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        <location>
            <begin position="1291"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1423"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1485"/>
            <end position="1499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1640" mass="178294" checksum="70C866420C530E3D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Y5</accession>
    <name>A0A1A0H9Y5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">DNA repair protein RAD16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_12642</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711205.1">
        <property type="nucleotide sequence ID" value="XM_018854247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Y5"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Y5"/>
    <dbReference type="GeneID" id="30027223"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16567">
        <property type="entry name" value="RING-HC_RAD16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000753">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001864">
        <property type="entry name" value="DNA repair protein RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="285"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="628"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="717"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="188"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="211"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA20683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="884" mass="99623" checksum="4400DDF3F6C3B512" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA01</accession>
    <name>A0A1A0HA01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_169908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711469.1">
        <property type="nucleotide sequence ID" value="XM_018854678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA01"/>
    <dbReference type="GeneID" id="30027654"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="137" mass="14844" checksum="321074812A8ADDF1" modified="2016-10-05" version="1">MAATRAAFCGLPGRQPFGFPGGLPVSERPFFPGGCLVFVWLSCFCGGFLVVQVAFCFRGAFCFHGAVCFSWGRRKRGPGKPRRSLAAASCLAGARQYIPSALCVPANKRWPGNREDRAANRCRLAAVWRANWGAAWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAF3</accession>
    <name>A0A1A0HAF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ras-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_42786</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711619.1">
        <property type="nucleotide sequence ID" value="XM_018857587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAF3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAF3"/>
    <dbReference type="GeneID" id="30030563"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000011">
        <property type="term" value="P:vacuole inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01862">
        <property type="entry name" value="Rab7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000086">
        <property type="entry name" value="Ras-related small GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="214" mass="23762" checksum="1A8133BF9B187CE9" modified="2016-10-05" version="1">MSSRKKTLLKVIILGDSGVGKTSLMQQFVNNKFSHQYKATIGADFLTKDLVIDNKTVSLQIWDTAGQERFQSLGVAFYRGADCCVLCYDVTNEKSLNNLPSWKDEFLIQLNVQNPQDFPFIVIGNKIDVDESKKIASLQKKLMHVTKNQLGGLNYPVFETSAKDSVNVESAFEVIAKMALQQEELTESSRNDAGDDYNDAINIHLENPAGGCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAN8</accession>
    <name>A0A1A0HAN8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">STE3-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_41272</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711453.1">
        <property type="nucleotide sequence ID" value="XM_018857376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAN8"/>
    <dbReference type="STRING" id="869754.A0A1A0HAN8"/>
    <dbReference type="GeneID" id="30030352"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14966">
        <property type="entry name" value="7tmD_STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00899">
        <property type="entry name" value="GPCRSTE3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="77"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="156"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="369" mass="41324" checksum="A2740C7B229E12CE" modified="2016-10-05" version="1">MSDAAGQILAAFSFLALAVLVPPLCYHVQQRNIPAASLIIWFCIQNLTTFINAVIWSGENYNRVYDGKIYCDITVRILSGATVGLLCATACIIFNLFMIIAAKNHGYLARDSKTKLAVNILMCWANPLLVMGLTVLAQNHRYDIARYYGCSAPWNYTLGSVMLVSIWNVIWIAVACVFAVLTIVELARKRKDILDILRCTNSGLNMRKFSRLIVLSFLIVLVFSPVTLISFAADVKAASSVTSEMAMAYASIKWSDVYAYDIGMNNLSSKVVPIVFSFITFFLFGLGTEALKMYRGFLVSIGFTRFKKEEPRDFYVAKDFDESRQTSKQTQSTEETVGLSQIEYEHFQTLVLEKEPFSLLPDSNDTASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ0</accession>
    <name>A0A1A0HAQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_169905</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711468.1">
        <property type="nucleotide sequence ID" value="XM_018854677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAQ0"/>
    <dbReference type="GeneID" id="30027653"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="146" mass="16358" checksum="B81B1DB52A8ACB39" modified="2016-10-05" version="1">MPLSYKPSRLVPFVTRQYRICQYEVGRRAQKNLAPLWCRPTLLASHQNPCMASHAIFSDNHSGNNHSILVETTPCPSATLAPSKNTTPSPRRKYPFLSVPNSAAALVVLQTPPWFQAFLPWHPCVSCTQALPELLWCHPARLAKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAW8</accession>
    <name>A0A1A0HAW8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Translation elongation factor EFTu/EF1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41287</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711650.1">
        <property type="nucleotide sequence ID" value="XM_018857377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAW8"/>
    <dbReference type="STRING" id="869754.A0A1A0HAW8"/>
    <dbReference type="GeneID" id="30030353"/>
    <dbReference type="OrthoDB" id="3969558at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44830">
        <property type="entry name" value="ELONGATION FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44830:SF1">
        <property type="entry name" value="TR-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP-eEF1A C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="112" mass="12114" checksum="BB7EC7EA023C6368" modified="2016-10-05" version="1" fragment="single">HPGQISSGYSPVLDCHAAHIACKFDTLLEKIDRRTGKSLESAPKFVKSGDPAIVKMVPTNPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVEKTDKDSKVTKAAQKAATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAY0</accession>
    <name>A0A1A0HAY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711547.1">
        <property type="nucleotide sequence ID" value="XM_018855892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAY0"/>
    <dbReference type="GeneID" id="30028868"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="68" mass="7562" checksum="AA9CBEEDD637668F" modified="2016-10-05" version="1">MYLESKLGLPPPVLFPFLLKKTSYFRASLEGCQSQPLTNNYRGQPIIDGSNGSDEGVSTNPPNYPKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB35</accession>
    <name>A0A1A0HB35_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA repair protein rad10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78162</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711602.1">
        <property type="nucleotide sequence ID" value="XM_018858836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB35"/>
    <dbReference type="STRING" id="869754.A0A1A0HB35"/>
    <dbReference type="GeneID" id="30031812"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="8">
        <location>
            <begin position="136"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="27"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="352" mass="37669" checksum="0FF2661721D75861" modified="2016-10-05" version="1">MGEPIDQSSFASILAGVQKMRRAYDVGVAAPAEPPQPAPTDEAPGLGRPPPRLEKPAQSPENTAQSLENTAQDTAQDTAQKPQAPSQKPPATAPKTAPKTAPKTAPQSAPKSAAHAAPLHASRRAGPTNVHSYTQVQVAPSQKGNPLLESPPMKLTPWAFNAQILLDYYINATVQVLFLSLKYHRLRPEYVWRRLERLRGGHASSSPAHDQALRVLLVVVDIDSPQEALRRLAVVCVKNDLSMVVAWLFEEAGAYVAALKLNEMARTKTASSIQGSRKADYNSSVVATLTAVRAVNKTDVVNLLANCKSFRNTVLQAALGDGVLDIPGLGGRKAANLSAAFAGPFVFNKDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBH0</accession>
    <name>A0A1A0HBH0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PAP2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_42839</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711743.1">
        <property type="nucleotide sequence ID" value="XM_018857592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBH0"/>
    <dbReference type="GeneID" id="30030568"/>
    <dbReference type="OrthoDB" id="10030083at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000810">
        <property type="term" value="F:diacylglycerol diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03390">
        <property type="entry name" value="PAP2_containing_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF35">
        <property type="entry name" value="RE23632P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="105"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="31713" checksum="71642E7549DA7029" modified="2016-10-05" version="1">MFDRVTFGVQNLTGGPIFIRWRMSDLIYVVILLILYPTLYRIDPFERQFYINDLTILHPFAETERVSNTMLFVYSTWVPLVVIFVMSLAITSRASKLYVTYVSVLGLIISVFTTSIMTDVLKNSFGRHRPDFLARCVPRPDTPKDVLVYAKDVCTTDNAARLMDGFRTTPLGHSSISFAGLFFLSLWLSGQLAVTRPQVGALRWVAVFLPTFGAALIALSRTEDYRHHFVDVFVGLCIGLAVATWLYMRVFPSPWAPDCYEPKLLDVDQPGINYTTVAEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBH6</accession>
    <name>A0A1A0HBH6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_78446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711999.1">
        <property type="nucleotide sequence ID" value="XM_018858882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBH6"/>
    <dbReference type="GeneID" id="30031858"/>
    <dbReference type="OrthoDB" id="4084018at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="308" mass="34500" checksum="E5A29F31BE05CF82" modified="2016-10-05" version="1">MDTKAHKLRLLLGRAPVPAPEPARSVSQEVYRRKLAADIARTINFIRQADDAIERLQKQLGPWPAVFNESAARTSEGPALPGPPWGLETCRQAHLLAHFEVYKQMPYQPMKNDLIGIATGLTLTLDAVVQQAAAAETLRRQNEATTAQIAHLAAVLSDYKRANTLVLERVREHPRRTAEMQRRIARVSSFGAEAADKLAHVQRLSDGATALEDRLHAHLLDVVTRLYAMMDWENAHVMDEDTFTKSIAGSVLLVKRLVSSVAEGTDKWVTVRPGTAEERLMEVMIRNDLLLHRDDRGDLEVRLRDYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBK0</accession>
    <name>A0A1A0HBK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711773.1">
        <property type="nucleotide sequence ID" value="XM_018855929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBK0"/>
    <dbReference type="GeneID" id="30028905"/>
    <dbReference type="OrthoDB" id="4034134at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038872">
        <property type="entry name" value="Put_GTT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41807:SF1">
        <property type="entry name" value="GLUTATHIONE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="351" mass="39224" checksum="DCFCC870B91DFA73" modified="2016-10-05" version="1">MSEFKSYRKADLAKIAAKIGADVRSKDTKTSLLEKIDQLMEQHPERTKALLDQDDDLEVVTLVDEHGASTESEDDSEGKSADESADEPATISAALAADDKDYNAPPPINLKEWLVDPAIAAYKAAHDQVLQLTDCVGMTALECNDDLRESLSKTVSLNFLEAAVEASHFLYFYVPLVPVKDNRSVHQLLRDNIPCLNLCSWPVPDVTALGNFSVMSILASWTLFAVALPLLISYYVNFSRRVVTFETSARLDDDEEEEEEEEEEDVLMVIRAYKYDPFIFALSKVLIHYFLHKNAALTSIESYQGLVHAVKNLVYIQLGIYHTFLANLGSFPLVLGLANVVVALYSQFEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBK4</accession>
    <name>A0A1A0HBK4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31817</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711767.1">
        <property type="nucleotide sequence ID" value="XM_018855927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBK4"/>
    <dbReference type="STRING" id="869754.A0A1A0HBK4"/>
    <dbReference type="GeneID" id="30028903"/>
    <dbReference type="OrthoDB" id="2142040at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706">
        <property type="entry name" value="SLR1338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706:SF1">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="31028" checksum="ECBA2CCC6F65A0C4" modified="2016-10-05" version="1">MSVHQPPPYSPVNDKLASKSAPVRNVPRAVTTSLIPESQLSALKGYDIVYLVDDSSLMAWREKISGIVPWPSARDALVSFSNLCAEWDDDGQDIYFINRPQGLKGCTPDEIAHAFDSAPPSGLTNMGRKLQQIAYEYFKDFDPIQTKPVNIIAITDGEFTDDVETVVRWIVKNLEKKNALANSFGIQFVQIGADIKAKKHLEYLDDNLSDLSRDIVDTVPWLEHRVDGRGFDGKYLLKAVCGAINKKLDNDNMNTRKKSGPDKQKRPGTFRRLFGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBN9</accession>
    <name>A0A1A0HBN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_42157</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711813.1">
        <property type="nucleotide sequence ID" value="XM_018857503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBN9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBN9"/>
    <dbReference type="GeneID" id="30030479"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF10">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="18"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="690" mass="77574" checksum="39F852C6F0B9D307" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBV6</accession>
    <name>A0A1A0HBV6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_41434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711975.1">
        <property type="nucleotide sequence ID" value="XM_018857398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBV6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBV6"/>
    <dbReference type="GeneID" id="30030374"/>
    <dbReference type="OrthoDB" id="6130531at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006693">
        <property type="entry name" value="AB_hydrolase_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04083">
        <property type="entry name" value="Abhydro_lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Partial AB-hydrolase lipase" evidence="2">
        <location>
            <begin position="86"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="507" mass="58614" checksum="DBA15955F1BD8EAA" modified="2016-10-05" version="1">MSNLNPTVRTDLHGPVLTFFIVLSSAIGSFLFVCSVTLGANIYHLRDIFSGREKANRPKKENAVANYSPVEPYPNVDKLKITRDLRYYAQLLDLDLEEREITTKDGYVLVLHRLIDPKESPETRDAKTPIILQHGLLLCAGAYLAPGRNSLPYFFLHQGYDVWMGNNRCGFKPNHLTLKGNLMHNEHFWDWDVRHLAYYDLPCIIDNVLLHKPNHAKVYLVGHSQGCTQTLLLLRNEQLKEHHAKIEHFFALAPAAFPGRLFHERSFIKFIHSKSPRMYKMIFGNCCFLAMLTQLRNLMGTTMFFSTLSYQIFKYLFGWNIRNCYNHKKVIHVQFLFNASLVSAKLMSWWLSYSVEEGFLNQLQLKQAYKDRTTADFTPLNSRDETVPIQNEDLEKNMGEIDDAHDKDSKTLFPYKAEWFSTTEKTNIVPMMVFVGGEDYLVDGLRFASHMKHYERHFYSQPKNLEVVELAEYNHLDVIWALNCIGTIGMPIVERINAQSGDASVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBY7</accession>
    <name>A0A1A0HBY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Winged helix DNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31934</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711913.1">
        <property type="nucleotide sequence ID" value="XM_018855948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBY7"/>
    <dbReference type="STRING" id="869754.A0A1A0HBY7"/>
    <dbReference type="GeneID" id="30028924"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="6">
        <location>
            <begin position="5"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="215"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="262" mass="30766" checksum="6FB687DD838A5724" modified="2016-10-05" version="1">MTESIYTGEDFEDKVKKQVEFYFSDSNLQTDKFLFKIYEANDGWVELKTILTFGRMRQYRPEEKAIAALKTSDKLVLSANEDMIRRKDPLKDYNEIKNTRKKNSVHIEGFPTTLLQEDVEEWFNSKILPTLPQEKAVCSIRRIRSRADKQFLGVVDVEFNTHEDAEYFLSVSATYADGIVSEESVEDKKDLLQKMSLLTFRQMRESGKRFGVNDVTKRRNSFKDNNRKKMRKSDKKDDEKVEEKVEEKVEEPKEEPKQEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC70</accession>
    <name>A0A1A0HC70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YCII-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_12078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712095.1">
        <property type="nucleotide sequence ID" value="XM_018854160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC70"/>
    <dbReference type="GeneID" id="30027136"/>
    <dbReference type="OrthoDB" id="5519740at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1060">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="109" mass="11938" checksum="F939C0E1F0C24C0F" modified="2016-10-05" version="1">MSKIEWNVIVFDKPGTDRTAVRPQHVAAIPESVNNGTVTSVGAIYKDASKTQFAGSTFHMMAESREEILDFLKKDIYYESGIWDLDSVIAHPVGIAARLGKAMPGVTKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC82</accession>
    <name>A0A1A0HC82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="3 11">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_42550</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="3 11"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712249.1">
        <property type="nucleotide sequence ID" value="XM_018857556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC82"/>
    <dbReference type="STRING" id="869754.A0A1A0HC82"/>
    <dbReference type="GeneID" id="30030532"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000050">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000044">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034762">
        <property type="entry name" value="Lys-tRNA-ligase_II_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039101">
        <property type="entry name" value="LysRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="248"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="589" mass="67643" checksum="DEF8496FFA8A8446" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCL4</accession>
    <name>A0A1A0HCL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712245.1">
        <property type="nucleotide sequence ID" value="XM_018854192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCL4"/>
    <dbReference type="GeneID" id="30027168"/>
    <dbReference type="OrthoDB" id="4013061at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="336" mass="36537" checksum="09A824EB3CB30140" modified="2016-10-05" version="1">MAIDCSSANNLRNTLDNLSISSSPEPVQDCESSSMHPQTTRSLLSKSLAGECHRESAAPDPQQFRIGEDDVYSSYGHPEPSLLATSIGKDHDGRLIPSSSTISLLSLNSNVNGGSLLTHPETFNTSVKGSVPGLTHANPDRVSSYTNLRSRNSITHLGQQRLQPYHKLSSPIAPSLGEFSIISSPKTIPWGKNQMTTPDSPNLNPTSFGESPSRFWLSAQTPPKSLTMPKHKNQFVGPLGFAVTPFQQKNQQKHAFTNTINITRGGDSPVLIPVQTPIEDIPMTPLYLNSEGSYFADSNGPRDYGEARADYFSGANESLLEEKEQALSDSEDHNMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCN9</accession>
    <name>A0A1A0HCN9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712188.1">
        <property type="nucleotide sequence ID" value="XM_018854180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCN9"/>
    <dbReference type="GeneID" id="30027156"/>
    <dbReference type="OrthoDB" id="3063476at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053203">
        <property type="entry name" value="Cisplatin_resist-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022024">
        <property type="entry name" value="DUF3602"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34693:SF1">
        <property type="entry name" value="PROTEIN PAR32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12223">
        <property type="entry name" value="DUF3602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="170" mass="18092" checksum="87C940CB2DC3D8A3" modified="2016-10-05" version="1">MVSTGRGGSGNIVSPEEVREERPEVPHEHKSDKRIYYSTGRGGSGNIKSSSEMPSPKLVPQGTNTPKLTTSKVTTGRGGYGNMVDNDDPNLTRKLQDVDGNKAENELLAVASNKSFAIGRGGFGNVVSKTRSNGSSAGSNPNNLYTVVSQGEKTSQKKGNFMLKVKNLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCQ6</accession>
    <name>A0A1A0HCQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 17">mRNA-capping enzyme subunit alpha</fullName>
            <ecNumber evidence="3 17">2.7.7.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 17">GTP--RNA guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 17">mRNA guanylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_137868</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16 17">Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the eukaryotic GTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="3 17"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712282.1">
        <property type="nucleotide sequence ID" value="XM_018854427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCQ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCQ6"/>
    <dbReference type="GeneID" id="30027403"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099122">
        <property type="term" value="F:RNA polymerase II C-terminal domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07895">
        <property type="entry name" value="Adenylation_mRNA_capping"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000542">
        <property type="entry name" value="mRNA-capping enzyme subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017075">
        <property type="entry name" value="mRNA_cap_enzyme_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036959">
        <property type="entry name" value="mRNA_cap_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 17" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9 17" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="5 17" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="20">
        <location>
            <begin position="44"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme C-terminal" evidence="21">
        <location>
            <begin position="245"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="382"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="N6-GMP-lysine intermediate" evidence="18">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR036959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR036959-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA21786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="448" mass="52799" checksum="8E45E94D3D93B944" modified="2016-10-05" version="1">MLKLGNRDMPVIPGTKIEGEELQELRLMLADLLRRKSTQFPGSQPVSFERNHIEVLKRREYFVCEKSDGLRCLLFLINDPVRGEGVFLITRENEYYFIPNIHFPLLADEEKGRTYHHGTVLDGELVMETKNVLEPFLRYCIFDALVIHEKDITNRPLPKRLGYITENVMKPFDSFKKRNPEIVNSPEFPFKVSFKMMKLSYRADVVLSMQDHLFHETDGLIFTCAETPYVFGTDATLLKWKPAHENTVDFKMELIFKMYQDPDMDSRDPDSTYPDYDSVPEEINLHVWKGGRDYEHFAQLDLPEDDWEQLKAIEEPLQGRIVECRKKLSKPPFWEMLRFRDDKSNGNHFSVVEKIIHSIEDGVTEKELVEACPVIEKACKQREAERMHKTRGPQIPEPLKRTSEHPIENKPRQRQEDIEQPHPKRRKVSEEEMLQEMMPDYEEESELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCR6</accession>
    <name>A0A1A0HCR6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_139613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712385.1">
        <property type="nucleotide sequence ID" value="XM_018854463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCR6"/>
    <dbReference type="GeneID" id="30027439"/>
    <dbReference type="OrthoDB" id="4086732at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="275"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="278"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="455" mass="51330" checksum="F6685F860A1C9B89" modified="2016-10-05" version="1">MSSLLRQDPFRLLPTERNSTRGTQSSERRKTDNGTSSIPADEFDDDAEMNTPHAIREASRPYCSNVLLSKSSSSVFVRRSEAADFLETPIKERNSRNDIVADGIDGNEPRLLHAGMDPVGYPELPPKEVLVDENNNLTTHPTSLSPPQKHLSGFERDLNRMEFDPSDYELPEKSMHALSSNRGMQKTVLPRPYALDESVRKRPQISSVPSYNLPNPPHNADRTPRRLSPLKNVLIPSSGSRSSPSTMLEEIRNSGALQPRTAREIQDQINSSLRQLEQSDLTSESQDSSSLSESEDDSDEDDSDEDDFLDHLGQYIPSVEKRKPTEHAPSVSEDQSTKTVEQNLPPADSLTAHLLPKNDALHRVKRSRTRRTGKLSGKYARKKIKDTRQKVSKKKYGDWLADQWDKLRRLLDLSVPNSVVANSYIVMRELNCTNKQELALRVKFLENQGKARAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD57</accession>
    <name>A0A1A0HD57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SAS complex, SAS5 subunit/transcription initiation factor IID, subunit 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30934</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712447.1">
        <property type="nucleotide sequence ID" value="XM_018855743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD57"/>
    <dbReference type="STRING" id="869754.A0A1A0HD57"/>
    <dbReference type="GeneID" id="30028719"/>
    <dbReference type="OrthoDB" id="1741717at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16905">
        <property type="entry name" value="YEATS_Taf14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016665">
        <property type="entry name" value="Sas5/TAF14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016551">
        <property type="entry name" value="SAS5/TFIID_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YEATS" evidence="3">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="224" mass="25189" checksum="9BC501B14AB115D2" modified="2016-10-05" version="1">MKDVPAVENYPMRQWSIEITMLNDQGAVIPATILDKVTYTLHPTFANPIRALKQPPFKVEEQGWGEFDIPIAVHLVGIPGKQGERKFNHDLNFLQETYTVDHVILVPLKNAQLNKLLAESGPVPLEGDAKRKSDAEVLSKLKKLKSTSSATKGSVDLEKLAKGLTLLSEDDLITIVQMVTDNRTSDMNIKNDFENGEFTMDLYTLPELLLKSLWEYVQRHTTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDB5</accession>
    <name>A0A1A0HDB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30945</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-25S rRNA processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM RBM34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712461.1">
        <property type="nucleotide sequence ID" value="XM_018855745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDB5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDB5"/>
    <dbReference type="GeneID" id="30028721"/>
    <dbReference type="OrthoDB" id="442677at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="155"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="262"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="22"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="328"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="446" mass="49551" checksum="D706E35981293A5E" modified="2016-10-05" version="1">MSAFSSLFSGAKVDETVNDLLNKSLGPVGRQDVRPRTVVEVPEETASTDKTSGSQAEGAVAKPVDASEPVEAPKKRKSLKKKRGDDDENEALEAEYYAKVTGEPESSAEEEQKVEAAGSDQEAPESSATKTPADRKLSVAKSVDLKEVELQKAERTVFVGNVSNKVISSKTTYKDFKKILRNIGSVESIRFRSIAFDEALPRKVAFARKNLHDSRDTLNAYVVYKEKEASLKAPERLNATVFDHNHIRVDHVAHPSPKDNKRTIFVGNLDFEEKEESLWRYFNSKVDDDVESVRVVRDAKTNMGKGFALVQFKDTLSVNKALLLNDKPMTSEGKKPRKLRISRAKNNAKPSVISPNHFDNMKKTQKPKARLTDDQKTKIGRAKKLLGKADRSSAGQMVAEGSRASKGQTIAGIKGLKSAQGRVKKPRITKRSTLFKQDRKKFENLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDH1</accession>
    <name>A0A1A0HDH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metallothionein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_146805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712625.1">
        <property type="nucleotide sequence ID" value="XM_018854529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDH1"/>
    <dbReference type="GeneID" id="30027505"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10597" checksum="C1C746EC5F9B3E31" modified="2016-10-05" version="1">MSCCASTHPSCKCEKCQGGNCQGAKCQCEKCQCDKCQCTKSCEGKECEAECEDKCKSENCLCDKCQCEKCQCGKCKSDNCLCSVCKCSTRPCEKCEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK0</accession>
    <name>A0A1A0HDK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_40044</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712499.1">
        <property type="nucleotide sequence ID" value="XM_018857222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HDK0"/>
    <dbReference type="GeneID" id="30030198"/>
    <dbReference type="OrthoDB" id="5273213at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="3">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="517" mass="56596" checksum="4C0678C8648F0812" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK1</accession>
    <name>A0A1A0HDK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="8">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_77498</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Endoplasmic reticulum membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PIGW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712468.1">
        <property type="nucleotide sequence ID" value="XM_018858710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDK1"/>
    <dbReference type="GeneID" id="30031686"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="55627" checksum="1D4E60F4A2E6D4F8" modified="2016-10-05" version="1">MSHKQLKEEFVSGLTGGSVQEIYAVTGTALAALLAHTCVQRWLVPRIPILTKFPVLFIVAFYFDVLIQLQAITIYGGRVLGLYYTAASIAIGAFVYGCIYVAGHKQHKQQKQQKQQKQQKQQKQQKQHKEASKRNTCLETLGQIPYITAYRSQMLVITNLAILAVDFHVFPRRFAKVETWGTSMMDLGVGLFVFSMGLANSRSLIKKSLAKHQNSQNYLRLLWGNTLKALPVLCLGLVRLVSVKSLDYQEHVSEYGVHWNFFMTLGLLPVFLGIVDPLFAVVPRFIVALAIGIVYECMLTHGGLGRYVLDESNRHDNLFTLNKEGLASFVGYLSIFIFGQSFGSFVLPTRKLPNNLVGIGLPQKPRRWLTVSPTQGLVIATLVLFGLFHVSAVSVFTGAISRRLANLPYVLWVVSYNAAMLLGYNLAERLAGPIDSKILNAINKNGLAIFLVANLATGLVNMGFDTLQASDGMAYSILIVYSLSWVGLALALDHYKVYIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDT7</accession>
    <name>A0A1A0HDT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_38779</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712750.1">
        <property type="nucleotide sequence ID" value="XM_018857044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDT7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDT7"/>
    <dbReference type="GeneID" id="30030020"/>
    <dbReference type="OrthoDB" id="274922at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000002">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11038:SF16">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="16"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="89" mass="9783" checksum="6D3A9515D4492EAD" modified="2016-10-05" version="1">MFGLGGSPQISSQQKIQAAEAELDMVTGMFNSLVEQCHAKCINKSYAEGDVNKQEALCLDRCVSKYFETNVQVGENMQKLGQSGQFMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDU0</accession>
    <name>A0A1A0HDU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase inhibitor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38703</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712755.1">
        <property type="nucleotide sequence ID" value="XM_018857034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDU0"/>
    <dbReference type="STRING" id="869754.A0A1A0HDU0"/>
    <dbReference type="GeneID" id="30030010"/>
    <dbReference type="OrthoDB" id="551302at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007062">
        <property type="entry name" value="PPI-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398:SF20">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY INHIBITOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12398">
        <property type="entry name" value="PROTEIN PHOSPHATASE INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04979">
        <property type="entry name" value="IPP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23762" checksum="AA55760989EA439A" modified="2016-10-05" version="1">MEPRGILRNKETKSAEETRAEEFDRQEVIRNTRLNAQLAAETNRGKEIRAKIAEAKGDSHDARALPEHLKWDELNLYQTEQEKAATMKIDEPKTPYEGGFDPEGEYYRDDGDEDTGPGEIPELLLGEGEYDMREVQSLSLNGGELINPGVGSPDGDEQGDAEPEAPALLAEERHKRFEEMRKAHYHNAANPLKHEIDIPDVDESEQGPDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDU7</accession>
    <name>A0A1A0HDU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_146361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712594.1">
        <property type="nucleotide sequence ID" value="XM_018854523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDU7"/>
    <dbReference type="GeneID" id="30027499"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="115" mass="12747" checksum="4BFB8833B2D5A76E" modified="2016-10-05" version="1">MSVDAWTVKLQSSCPLCFLGSIFHLAVRDFSCHGSCMSRMACVCSRLDARVVDMSNMKAPSVESNLPAHGWYPRLITFYFFFGTASLVSGTYVSRAFTIHSLCTVSQCFDQVTPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ4</accession>
    <name>A0A1A0HDZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">histidinol-phosphate transaminase</fullName>
            <ecNumber evidence="4">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_40133</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712815.1">
        <property type="nucleotide sequence ID" value="XM_018857235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDZ4"/>
    <dbReference type="GeneID" id="30030211"/>
    <dbReference type="OrthoDB" id="2015537at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="12">
        <location>
            <begin position="29"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="400" mass="43627" checksum="8AB2FCBBA964E6B2" modified="2016-10-05" version="1">MTFDLQSLVRPNIAKLEPYRCARDDFKEGILLDANENTHGPALETVSSYEAALELNRYPDPHQMALKQQIVAFRSAQNSRHLADPQTHGLVPENLCLGVGSDESIDLLLRCVCVPGKDKLLVCPPTYGMYSICAAVNDVALVRVPLTAPGFQLDSDAVLHAVTQDPAIKLMYLTSPGNPTGQLLQVAAILALVAKLRGVWNGLVVVDEAYIDFASEEDGSSPSVSTLVNQHPNLVVMQTLSKSFGLAGIRLGITYACRELAHYLNAMKYPYNISSLTSAVAMRSTCPDTGLQVMERYVALILRERALLLLRLQELEGVGRNLGGLDANFLLVEILDKDGQPSNAVAHAAYTKLAVDNAVVVRFRGKELNCTGGLRISVGTQEENRELLERFAKCLVEARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE53</accession>
    <name>A0A1A0HE53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable metalloprotease ARX1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Associated with ribosomal export complex protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_77802</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable metalloprotease involved in proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Accompanies the pre-60S particles to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712885.1">
        <property type="nucleotide sequence ID" value="XM_018858770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE53"/>
    <dbReference type="STRING" id="869754.A0A1A0HE53"/>
    <dbReference type="GeneID" id="30031746"/>
    <dbReference type="OrthoDB" id="5876363at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047113">
        <property type="entry name" value="PA2G4/ARX1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804:SF102">
        <property type="entry name" value="METALLOPROTEASE ARX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10804">
        <property type="entry name" value="PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="13">
        <location>
            <begin position="30"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="558" mass="60428" checksum="251283B3B04F7F65" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEA1</accession>
    <name>A0A1A0HEA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TPR-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_77761</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712817.1">
        <property type="nucleotide sequence ID" value="XM_018858757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEA1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEA1"/>
    <dbReference type="GeneID" id="30031733"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="201" mass="23020" checksum="DC71D1931375B3F6" modified="2016-10-05" version="1">MEYDLRYDAKSKGMVFKQQPEHVTALLQLELEQTNTLISELMNLAAAVPPKASPETFNKDLSKMIKKLYESGVALFKQGKYTESAKQFSIALEIANRRSKFEAFQGTLQEISLLLMSRADAHLKCKEYLKAFNDADMLISMMVCTPDNFLRRGVANYFLANYEDARADYQRGLAFDEKNERLQKELDTCLARILEENGDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEQ3</accession>
    <name>A0A1A0HEQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Multicopper oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_40107</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712977.1">
        <property type="nucleotide sequence ID" value="XM_018857231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEQ3"/>
    <dbReference type="GeneID" id="30030207"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13850">
        <property type="entry name" value="CuRO_1_Abr2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF488">
        <property type="entry name" value="LACCASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0410">Iron transport</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291776" description="Multicopper oxidase" evidence="8">
        <location>
            <begin position="21"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="11">
        <location>
            <begin position="35"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="9">
        <location>
            <begin position="175"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="10">
        <location>
            <begin position="494"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="68093" checksum="1504DA7EB51F5BFE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HER8</accession>
    <name>A0A1A0HER8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_11432</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713115.1">
        <property type="nucleotide sequence ID" value="XM_018853982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HER8"/>
    <dbReference type="STRING" id="869754.A0A1A0HER8"/>
    <dbReference type="GeneID" id="30026958"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF121">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="581"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="606"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="249"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="359"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="642"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="312"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="664"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="692"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="701" mass="78742" checksum="B4D3BE8B1B7D5EFD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFI1</accession>
    <name>A0A1A0HFI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713138.1">
        <property type="nucleotide sequence ID" value="XM_018856834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFI1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFI1"/>
    <dbReference type="GeneID" id="30029810"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF8">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="6">
        <location>
            <begin position="4"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="127" mass="14288" checksum="3913D5D8AE4A534F" modified="2016-10-05" version="1">MKTDQEWKAVLSPEQFRVLRQLGTEAPFTGEYVKTPAGEGEYLCVGCDQPLYKANTKFPAHCGWPAFYEAIPGSIKTIEDKLLGMTRTEMRCSKCDGHLGHIFKGEGYKTPTDERHCVNSICLKLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY5</accession>
    <name>A0A1A0HFY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase subunit 8, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cytochrome c oxidase polypeptide VIII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_94133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of a catalytic core of 3 subunits and several supernumerary subunits. The complex exists as a monomer or a dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion inner membrane</location>
            <topology evidence="1 7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the cytochrome c oxidase VIIc family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713376.1">
        <property type="nucleotide sequence ID" value="XM_018859384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY5"/>
    <dbReference type="GeneID" id="30032359"/>
    <dbReference type="OrthoDB" id="9974841at2759"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.49.10">
        <property type="entry name" value="Cytochrome c oxidase subunit VIIc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004202">
        <property type="entry name" value="COX7C/Cox8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036636">
        <property type="entry name" value="COX7C/Cox8_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02935">
        <property type="entry name" value="COX7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU368123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="9063" checksum="E0266A9A4DEB6B9C" modified="2016-10-05" version="1">MISRVGMRAPRATRNFQTSAVTMNSVYGSPKTGIYSNLPFKVKDRKIPFGIVWWGTYGFFFAFPFLTSYWHMKKSGNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGI5</accession>
    <name>A0A1A0HGI5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713586.1">
        <property type="nucleotide sequence ID" value="XM_018856706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGI5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGI5"/>
    <dbReference type="GeneID" id="30029682"/>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="257" mass="29152" checksum="A76C0D14524C2765" modified="2016-10-05" version="1" fragment="single">MSSSISYLTVYNAHNGVAAKIPKPVRFSSLQKLKNYLRESFTDDIIGSTDNVFLLTPFGIKLDFAMINEMPELYLYDRRLFQKVAERALLVRYLDQANNPPIASLKPEPLSLFGAPSGPASPQNIKELLTSLKTYEKWTDHVALNLRHISDRIDVIIRQINVVFKALNVILLFASNFVGSAEKSFKSQFNHVKMLAMKSLNKSWKSYAASLEEFPPFRLKDGSVFRLADYVDHNSFSEAARYVSEHLPGVVLQFNDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGN5</accession>
    <name>A0A1A0HGN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Structural maintenance of chromosomes protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_76224</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMC family. SMC2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713636.1">
        <property type="nucleotide sequence ID" value="XM_018858498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGN5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGN5"/>
    <dbReference type="GeneID" id="30031474"/>
    <dbReference type="OrthoDB" id="10255539at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034506">
        <property type="term" value="C:chromosome, centromeric core domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990814">
        <property type="term" value="F:DNA/DNA annealing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003680">
        <property type="term" value="F:minor groove of adenine-thymine-rich DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903342">
        <property type="term" value="P:negative regulation of meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03273">
        <property type="entry name" value="ABC_SMC2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000278">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000385">
        <property type="entry name" value="Structural maintenance of chromosomes 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027120">
        <property type="entry name" value="Smc2_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="521"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="326"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="417"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="714"/>
            <end position="981"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1170" mass="132158" checksum="02D3B848753EFCA5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS5</accession>
    <name>A0A1A0HGS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_80583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713563.1">
        <property type="nucleotide sequence ID" value="XM_018859200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS5"/>
    <dbReference type="GeneID" id="30032176"/>
    <dbReference type="OrthoDB" id="4070686at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="279" mass="31865" checksum="D2C2833A8041E8FE" modified="2016-10-05" version="1">MSNMGLSVVNKLLKPSLPITENSSGVRCLHNSPISFFEINSLNPRWIYLRFDKVLPIVLLTPKLKHLSFGAKSEEFLCFLDAIKYATKKEVQISNTTFDLIDNLPLCLGAMLNESEVSYDHLNRVISSLNDTFGAANLLSLSEDTKSVIEPILPHCLRLLATDQIHSILGMWYSSYGEKKKLKFLFMLDKGKREFHKAGVIDFIADNLNRKRLSKVTTHEMKMIGHMFQVVSHDFSHIAKQFIQQIVLLKAEELSITVVIKSKLFDRMTLLRKRQALIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGT0</accession>
    <name>A0A1A0HGT0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF1445-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-glutamate cyclase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713568.1">
        <property type="nucleotide sequence ID" value="XM_018859380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGT0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGT0"/>
    <dbReference type="GeneID" id="30032355"/>
    <dbReference type="OrthoDB" id="10262538at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047820">
        <property type="term" value="F:D-glutamate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2040.10:FF:000001">
        <property type="entry name" value="D-glutamate cyclase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1640.10">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2040.10">
        <property type="entry name" value="PSTPO5379-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009906">
        <property type="entry name" value="D-Glu_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038021">
        <property type="entry name" value="Putative_hydro-lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016938">
        <property type="entry name" value="UPF0317"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32022:SF10">
        <property type="entry name" value="D-GLUTAMATE CYCLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07286">
        <property type="entry name" value="D-Glu_cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029755">
        <property type="entry name" value="UCP029755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160920">
        <property type="entry name" value="PSTPO5379-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="269" mass="30308" checksum="9B37CD4DE2B006D2" modified="2016-10-05" version="1">MTPTEFRQQCRQGLHTSQTSGFCPGFAQANLVILPTKVAADFHQLCLRNPIPCPLLAHTEIGNQHSTNDKRIISDKYFDITRDFPRYTVYENGSLVRCPTDVSDIWDPTRHVGFLIGCSFSFEKALDEAGLPPRNYSQKRNVSMYKTTRRLQDSGVFVDVPYVVSMRPYKLKDISRVKEITGRFKKTHGAPIDFGYDAMERLGIRDLSNPDFGDSTQIEDDEVPVFWGCGVTAQIAALSVHDRIDGPIIGHAPGHMLVTDLRDEDVMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGU2</accession>
    <name>A0A1A0HGU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PQ-loop-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37569</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713875.1">
        <property type="nucleotide sequence ID" value="XM_018856890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGU2"/>
    <dbReference type="GeneID" id="30029866"/>
    <dbReference type="OrthoDB" id="19344at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF11">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="30945" checksum="A27B5D8BC3C661FF" modified="2016-10-05" version="1">MINDSGAPADFCSVYDGASITNLVISVLITAGIFVSYLPQYFRIYKKKTSEGLSVSFLLLGSCSLIFTFTNIIMISSRARFCCRSGALTLFNCINSQLNLIQIGLQCTCAIMILVFVIVWTRGSMKQDKEEFSRILRVSYLVMLHAALSATQIVIAFSTSRSVLLALAQFNGLMSTLLTVIKYVPQILTTYRLKHPGTLSIGMMCIQTPGGFIFTATLFFTKGSHWSSWMSYLVAALLQGTLLALCIFYEHNSGYEDLERAEVERIEHENALEANGLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGX6</accession>
    <name>A0A1A0HGX6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713726.1">
        <property type="nucleotide sequence ID" value="XM_018855476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGX6"/>
    <dbReference type="GeneID" id="30028452"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="117" mass="13744" checksum="556BB09B7C1726CA" modified="2016-10-05" version="1" fragment="single">MDKAIRSFVHPSLQLRIICPAQDPTERHSRFSMPSLHRTNPQQRRRHRRFPKPYLQQLRNIQILVARAKIYQAHELELNVGPSRQLIGELNKIKLVRHHDLQGLHQKAWRVAGSGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGZ2</accession>
    <name>A0A1A0HGZ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_37082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713599.1">
        <property type="nucleotide sequence ID" value="XM_018856818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGZ2"/>
    <dbReference type="GeneID" id="30029794"/>
    <dbReference type="OrthoDB" id="4018341at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="215" mass="24894" checksum="59D88D87BE7E9991" modified="2016-10-05" version="1" fragment="single">LPAVQPVSTIFGLSHEQIYKTRLSDVKVKTIVNVPMSKMCKNALTFVMAENEGLFVDAEKTGQFVDLCTKVALYEAPGFNEVTKRIPALLDWVPQYEPLTLQHTKTNVTVSNKQIHKNCLDYTVFSFFGHILLWANELQHTAKATTIVEKYHLDISRTQILNSLGGYHLWDRLRRDKTINTKRWKHIQKFRQLFAFEEHQFVLILRCMNLGVPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHC7</accession>
    <name>A0A1A0HHC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PH-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_64492</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714065.1">
        <property type="nucleotide sequence ID" value="XM_018858345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHC7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHC7"/>
    <dbReference type="GeneID" id="30031321"/>
    <dbReference type="OrthoDB" id="73680at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13316">
        <property type="entry name" value="PH_Boi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09535">
        <property type="entry name" value="SAM_BOI-like_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000230">
        <property type="entry name" value="Polarized growth protein (Boi2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045188">
        <property type="entry name" value="Boi1/Boi2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902:SF27">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22902">
        <property type="entry name" value="SESQUIPEDALIAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="SAM" evidence="7">
        <location>
            <begin position="166"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="483"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="286"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="656"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="660"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="688"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="708"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="745"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="796"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="807"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="835" mass="91330" checksum="B7150251B5A9E3B9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHE5</accession>
    <name>A0A1A0HHE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_103101</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713744.1">
        <property type="nucleotide sequence ID" value="XM_018853734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHE5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHE5"/>
    <dbReference type="GeneID" id="30026710"/>
    <dbReference type="OrthoDB" id="10252687at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="462"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="681" mass="75417" checksum="BCCF6A8942A39E90" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHS3</accession>
    <name>A0A1A0HHS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S acidic ribosomal protein P0</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_110916</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714190.1">
        <property type="nucleotide sequence ID" value="XM_018853917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHS3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHS3"/>
    <dbReference type="GeneID" id="30026893"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="6">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="230" mass="24802" checksum="7BADDCB0BEA03DD6" modified="2016-10-05" version="1">MGGTRDKKVQYFAKLKELLTEYKSIFVVGVDNVSSQQMHETRKSLRGEATVLMGKNTMVRRAIRGFLSELPEYEKLLPFIKGNVGFIFTNSDLKTIREAIVSNVVAAPAKAGAVAPKDVWVPAGNTGMEPGKTSFFQALGVPTKIARGFIEIVSDVKVVEAGNKVGPSEASLLNLLGISPFTYGLTVSQVYDNGQVFPASILDITDDELVSHFVSAIQTIASVSLAVVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI27</accession>
    <name>A0A1A0HI27_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30091</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714137.1">
        <property type="nucleotide sequence ID" value="XM_018855554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI27"/>
    <dbReference type="STRING" id="869754.A0A1A0HI27"/>
    <dbReference type="GeneID" id="30028530"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF80">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="201" mass="22913" checksum="8CE56E994BC0AB7A" modified="2016-10-05" version="1">MSTGIELKDLHNKPFFDIEENTKFHRPAKAYVYLAVELVIGTQILYSYDHVALKFPLLAPAILGSTSAALAQSLIQFFRQKISTNRLLKFIAWGCINGCFTALWIDVLVSRVENVALQIIIDQTVGAPLFQLMFTVLSSIWENDSIFGLSSQSTFMKSLRYSYCYWPFISTSMFILVPPQLMFFLNCLASFVWNLILSRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI46</accession>
    <name>A0A1A0HI46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_64421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP domain-containing ribonucleoprotein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714038.1">
        <property type="nucleotide sequence ID" value="XM_018858344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI46"/>
    <dbReference type="STRING" id="869754.A0A1A0HI46"/>
    <dbReference type="GeneID" id="30031320"/>
    <dbReference type="OrthoDB" id="445357at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.30">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003034">
        <property type="entry name" value="SAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036361">
        <property type="entry name" value="SAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052240">
        <property type="entry name" value="SAP_domain_ribonucleoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040746">
        <property type="entry name" value="THO1_MOS11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46551:SF1">
        <property type="entry name" value="SAP DOMAIN-CONTAINING RIBONUCLEOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02037">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18592">
        <property type="entry name" value="Tho1_MOS11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00513">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68906">
        <property type="entry name" value="SAP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50800">
        <property type="entry name" value="SAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAP" evidence="4">
        <location>
            <begin position="4"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="13"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="37"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="177" mass="19358" checksum="16A3B46A7DB5F335" modified="2016-10-05" version="1">MSQYASLTVAQLKDALKEKNLPTDGKKADLVARLEEAAPATEEAEPNTQENVEEQQPQQETSAPAEAGAEAEIAEAEDEKPNGSEEKKPEKKVLTPEERKALAVDLLTKKIRRAEKFGDEASATAAKQDMARVEKFGVDPSTALGKEIGALDRDMNSELATRKARRSEKKGNRVQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIX9</accession>
    <name>A0A1A0HIX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714451.1">
        <property type="nucleotide sequence ID" value="XM_018855619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIX9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIX9"/>
    <dbReference type="GeneID" id="30028595"/>
    <dbReference type="OrthoDB" id="2195431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF3">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="202"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="312"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="807" mass="91722" checksum="E3C73194AF62F5A7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ20</accession>
    <name>A0A1A0HJ20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_114600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714363.1">
        <property type="nucleotide sequence ID" value="XM_018853995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ20"/>
    <dbReference type="GeneID" id="30026971"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="12860" checksum="37A1900CA7E53C48" modified="2016-10-05" version="1">MVQCSEALYSILVVQCSGALYPNILPGPVALFSNILPGPCSGTHSYICPGPASGPRIFLFPSPSALFSLAQHSRPSIPIPAGPSIPIPAGPSIPIPAGPSIPIPQALFFVFSRPRISLGFFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ66</accession>
    <name>A0A1A0HJ66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714364.1">
        <property type="nucleotide sequence ID" value="XM_018855590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ66"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ66"/>
    <dbReference type="GeneID" id="30028566"/>
    <dbReference type="OrthoDB" id="4094887at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017956">
        <property type="entry name" value="AT_hook_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="SMART" id="SM00384">
        <property type="entry name" value="AT_hook"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="490"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="523" mass="58933" checksum="7BA15B817B146A22" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ69</accession>
    <name>A0A1A0HJ69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA hydrolase</fullName>
            <ecNumber evidence="4">3.1.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetyl-CoA deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_10136</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Presumably involved in regulating the intracellular acetyl-CoA pool for fatty acid and cholesterol synthesis and fatty acid oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + H2O = acetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="3.1.2.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyl-CoA hydrolase/transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.1" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714413.1">
        <property type="nucleotide sequence ID" value="XM_018853691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ69"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ69"/>
    <dbReference type="GeneID" id="30026667"/>
    <dbReference type="OrthoDB" id="10250396at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003986">
        <property type="term" value="F:acetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006083">
        <property type="term" value="P:acetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.70:FF:000002">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000003">
        <property type="entry name" value="Acetyl-coA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.20:FF:000001">
        <property type="entry name" value="Acetyl-CoA hydrolase Ach1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.70">
        <property type="entry name" value="4-hydroxybutyrate coenzyme like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.20">
        <property type="entry name" value="Acetyl-CoA hydrolase/transferase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026888">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038460">
        <property type="entry name" value="AcetylCoA_hyd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046433">
        <property type="entry name" value="ActCoA_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003702">
        <property type="entry name" value="ActCoA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43609:SF1">
        <property type="entry name" value="ACETYL-COA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13336">
        <property type="entry name" value="AcetylCoA_hyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02550">
        <property type="entry name" value="AcetylCoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase N-terminal" evidence="10">
        <location>
            <begin position="10"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Acetyl-CoA hydrolase/transferase C-terminal" evidence="11">
        <location>
            <begin position="328"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="523" mass="57944" checksum="D0AC8CB7CBC072E9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJK6</accession>
    <name>A0A1A0HJK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_34958</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714676.1">
        <property type="nucleotide sequence ID" value="XM_018856545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJK6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJK6"/>
    <dbReference type="GeneID" id="30029521"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="404"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="5">
        <location>
            <begin position="526"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="749" mass="87454" checksum="8AE34A7E27B48353" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJU2</accession>
    <name>A0A1A0HJU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ, mitochondrial</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Glutamate N-acetyltransferase</fullName>
                <shortName evidence="10">GAT</shortName>
                <ecNumber evidence="10">2.3.1.35</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Ornithine acetyltransferase</fullName>
                <shortName evidence="10">OATase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="10">Ornithine transacetylase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">Amino-acid acetyltransferase</fullName>
                <ecNumber evidence="10">2.3.1.1</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">N-acetylglutamate synthase</fullName>
                <shortName evidence="10">AGS</shortName>
            </alternativeName>
        </domain>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="10">Arginine biosynthesis bifunctional protein ArgJ beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_117304</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="10">The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ArgJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="10"/>
    <dbReference type="EC" id="2.3.1.1" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714564.1">
        <property type="nucleotide sequence ID" value="XM_018854072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJU2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJU2"/>
    <dbReference type="GeneID" id="30027048"/>
    <dbReference type="OrthoDB" id="2017946at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.340:FF:000002">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2330.10:FF:000001">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.70.12:FF:000002">
        <property type="entry name" value="Arginine biosynthesis bifunctional protein ArgJ, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2330.10">
        <property type="entry name" value="arginine biosynthesis bifunctional protein suprefamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 10" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5023507966" description="Arginine biosynthesis bifunctional protein ArgJ alpha chain" evidence="10">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023507967" description="Arginine biosynthesis bifunctional protein ArgJ beta chain" evidence="10">
        <location>
            <begin position="292"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="518"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="10">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="10">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="518" mass="54725" checksum="7051AF13295EFAFF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK11</accession>
    <name>A0A1A0HK11_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thymidylate synthase</fullName>
            <ecNumber evidence="4">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_131966</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714841.1">
        <property type="nucleotide sequence ID" value="XM_018854317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK11"/>
    <dbReference type="STRING" id="869754.A0A1A0HK11"/>
    <dbReference type="GeneID" id="30027293"/>
    <dbReference type="OrthoDB" id="766at2759"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000005">
        <property type="entry name" value="Thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="11">
        <location>
            <begin position="9"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="315" mass="35907" checksum="78B134D5F71EE00E" modified="2016-10-05" version="1">MSKVNTAEQAYLDLCKRIIDEGEFRPDRTGTGTRSLFAPPQLRFDLSNDTFPLLTTKKVFSKAIIHELLWFVEGNTDANVLSEKGVKIWEGNGLREYLDKLGLTDRRVGDLGPVYGFQWRHFGAEYKSCDDDYTGQGFDQLQDVIHKLKTNPYDRRIIMSAWNPPDFAKMALPPCHVFCQFYVSFGSAGPTANTEKLAKPAQKPKLSCLLYQRSCDMGLGVPFNIASYALLTRMIAHVVDMDCGEFVHTLGDAHVYLDHVDALETQVQREPMAFPKLKIKEERKSEIKNIDDFKFEDFEIVDYQCHGPIKMKMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKC3</accession>
    <name>A0A1A0HKC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 16">ADP-ribosylation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_10407</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP-binding protein involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="function">
        <text evidence="16">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714734.1">
        <property type="nucleotide sequence ID" value="XM_018853761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKC3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKC3"/>
    <dbReference type="GeneID" id="30026737"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003500">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13 15" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 16" id="KW-0519">Myristate</keyword>
    <keyword evidence="5 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00070396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU369003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA24253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20579" checksum="1E002155B32B3580" modified="2016-10-05" version="1">MGLSISKLFSNLFGNKEMRILMVGLDGAGKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRYYYQNSQGIIFVVDSNDRDRIAEAREELQQMLNEDELRNALLLVLANKQDLPNAMNAAEITEKLGLHSIRQRPWYIQSTCATTGDGLYDGLDWLSNNLKKSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKC4</accession>
    <name>A0A1A0HKC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_131228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714818.1">
        <property type="nucleotide sequence ID" value="XM_018854308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKC4"/>
    <dbReference type="STRING" id="869754.A0A1A0HKC4"/>
    <dbReference type="GeneID" id="30027284"/>
    <dbReference type="OrthoDB" id="10017054at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11915">
        <property type="entry name" value="SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="7"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="130"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="632" mass="72224" checksum="899C19E8FA1D7985" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKH0</accession>
    <name>A0A1A0HKH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714967.1">
        <property type="nucleotide sequence ID" value="XM_018855695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HKH0"/>
    <dbReference type="GeneID" id="30028671"/>
    <dbReference type="OrthoDB" id="48509at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="3">
        <location>
            <begin position="154"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="512"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="816"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="573"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="470"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="841" mass="90949" checksum="DEB9C7A92E267A80" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKH1</accession>
    <name>A0A1A0HKH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide hydrolase</fullName>
            <ecNumber evidence="1">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30674</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714873.1">
        <property type="nucleotide sequence ID" value="XM_018855680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKH1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKH1"/>
    <dbReference type="GeneID" id="30028656"/>
    <dbReference type="OrthoDB" id="5841748at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF46">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="3">
        <location>
            <begin position="371"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="805" mass="90698" checksum="84C300CD767BD384" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GSB2</accession>
    <name>A0A1A0GSB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_48200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000034">
        <property type="protein sequence ID" value="OBA14616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709220.1">
        <property type="nucleotide sequence ID" value="XM_018858277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GSB2"/>
    <dbReference type="STRING" id="869754.A0A1A0GSB2"/>
    <dbReference type="GeneID" id="30031253"/>
    <dbReference type="OrthoDB" id="4070698at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56988">
        <property type="entry name" value="Anthrax protective antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291656" description="PA14 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="PA14" evidence="2">
        <location>
            <begin position="42"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA14616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="219" mass="23747" checksum="3575BFA9995345C5" modified="2016-10-05" version="1" fragment="single">MLIPSFFFTAAIVGIVSASAGCTPSMVGEKGFIANFYSYEFLGGKGWEKDFFVSGYKSDLLETVRCVESINFQYSNQPDQKVLHQTIYGYSTTISNYSLELSGYFEAPETGLYRFRLAADNGASLQFGAGQTCCNDASGSESGGFSFKTLGPRGGGGSTKVNVRDMSYSLVKGVYYPVKLVMFNWQGNNGLNLKVTKPSGNIISDFGSQVFQAEFANSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ93</accession>
    <name>A0A1A0GZ93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cation efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_33847</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709314.1">
        <property type="nucleotide sequence ID" value="XM_018856407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ93"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ93"/>
    <dbReference type="GeneID" id="30029383"/>
    <dbReference type="OrthoDB" id="9944568at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 63C, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="9">
        <location>
            <begin position="9"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="139"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA17017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="394" mass="43754" checksum="294ED6C54B08AC91" modified="2016-10-05" version="1">MKAKEIRIITLLVVDIFFFLLEAIVGYTVHSLALIADSFHMLNDIISLFIALWAVKVKNSKPADGKYTYGWQRAEIIGALVNAVFLLALCFTIVIEAIQRFFAPPEISNPQLILMVGFFGLLSNGLGLVLFHEHGHSHGHSHGSGSSNQSHSHSHASGSSDVHDEESNIRSGSYTENNRSSTDISEYLPDTVVARHDERSNLLSGNKKDVPRKKLKSMNMEGVFLHVLGDALGNVGVIITAIFIWKTDYSWRFYSDPVVSLFITLIIFSSALPLCRKSCKILLQATPQNLDSNVIVEEITLLPSIKAIHDFHVWNLDEDFLVASLHIELNEQPESNIHDSKGDGKIDKNAFVETVAQVREILFRSGISSVTIQPEFGNKRVNSVYGVNKTPSCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1X7</accession>
    <name>A0A1A0H1X7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">Peroxisomal membrane protein PEX14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Peroxin-14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_33724</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor. The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm. Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9 10">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peroxin-14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709350.1">
        <property type="nucleotide sequence ID" value="XM_018856387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1X7"/>
    <dbReference type="STRING" id="869754.A0A1A0H1X7"/>
    <dbReference type="GeneID" id="30029363"/>
    <dbReference type="OrthoDB" id="5549158at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025655">
        <property type="entry name" value="PEX14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006785">
        <property type="entry name" value="Pex14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23058:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04695">
        <property type="entry name" value="Pex14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="3 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peroxisome membrane anchor protein Pex14p N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="339"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="298"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA17955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="380" mass="42253" checksum="0EA4C6559404D30E" modified="2016-10-05" version="1">MNEDLINSAVSFLQDLTVAASPINKKIEFLQTKGLNEEEIDEAIKRANNSPPNPSSSPQTSSATTYRGAVQEQPHHAQNYAPLDYYSVAPQVPERTWKDYFIMATATAGVTYGFYQVVSRYLLPSIIPPGRSRLEDDKQVIEDEFIKIDKVLEQMALEQKSIKDANEAKLLEIDEAIGNVNRFLEKYDKDKLKLDDDLRLVKLEVDNLQNFLEKNMKLTKENIRDELTDISGELESLKSLIKARGATSGSVDRKVAPVSSIPSASEILKKAKSNGNGSSALAKPPVSAELPMGKEAENQNTTLESSQAPPQKAPKSLRVPSFTANKEFLAAGIPDWQLAHKKQEEESSVKEDEKKELKGEQKVQESILRVGVPQWQLNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Y0</accession>
    <name>A0A1A0H1Y0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_13877</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709355.1">
        <property type="nucleotide sequence ID" value="XM_018854440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Y0"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Y0"/>
    <dbReference type="GeneID" id="30027416"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006598">
        <property type="term" value="P:polyamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000001">
        <property type="entry name" value="Aldehyde dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000012">
        <property type="entry name" value="Betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43720:SF2">
        <property type="entry name" value="2-AMINOMUCONIC SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="29"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA17960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="54450" checksum="35E017C9D930CE4B" modified="2016-10-05" version="1">MSLPLEYQVTLPNGRTFTQPAGLFVNNEFVKSKSGKTIESINPSTGEVNGSVYAADEEDVDEIVAVARKAFKSWKKTSGVERADLLFKLVDIMKQELELLTDIEAWDSGKTRHLNALYDVEEVIAVFKYYAGWADKVHGRVIAQNPKKLAYTLHEPYGVCAQIIPWNYPLLMAAWKLAPALAAGNVVILKSSEVTPLSILYFATLFKKAGFPPGVVNIISGLGTTAGKALASHLDVNKVAFTGSTATGRVVQKLATANLKAVTLECGGKSPLLVRADADLEQAVKWAAIGIMSNQGQICTSTSRIYVHESIYEKFLTEFSKHVTDEYKQGDMFVEDAVVGPQVSKGHFEKILSYLDIGKKEGARVVLGGEANTEGELAKGYYVKPTVFADVQPHFRIVKEEIFGPVVVVGKFSTDEEAIELANATEYGLGASIFTQDITVAHNMAADIESGQIWINSSNDSDVHIPFGGVKMSGVGRELGEYGLEMYTEAKAVHVNLGTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Y4</accession>
    <name>A0A1A0H1Y4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709436.1">
        <property type="nucleotide sequence ID" value="XM_018854458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Y4"/>
    <dbReference type="GeneID" id="30027434"/>
    <dbReference type="OrthoDB" id="4095869at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="362" mass="39211" checksum="0985FA6F79A10D05" modified="2016-10-05" version="1">MVLPKEAEETIALLFPPENDPAASPGPQRTGTPTPYTVYPEELLHATPNGSKCYVGLQSLTRLFMDALGEENRAQSPGGAGPNRHSIVQFGLSRLSALLRQKVHLWDETDADHAGGPRDAPGRMSVSGSSAASSLFAWSSGDKYIAARKRELQQQRELAQRELPRRNGSDPARPEPEPAVPRTAGMAAWGLAPLISAGPLISAGPLTSASPRGPETPLTVVLRTEPPSRPQLDTSPAGRATSKPELRETGPPQSEPLIRAAPSSGPPLARPQKSNLGHRLAAVVQRESNRFIQDRIDLIKAHHLKQASRKLDERRRRDHEMHLHRVQLKEEKDRHGREHSAPGRKTRSAGVSSARPGPGLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H260</accession>
    <name>A0A1A0H260_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">PIN domain-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_47783</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018858220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H260"/>
    <dbReference type="STRING" id="869754.A0A1A0H260"/>
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    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="11">
        <location>
            <begin position="138"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="359"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="571"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="382"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="500"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA18043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="660" mass="71213" checksum="1967C3245536DA35" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4U5</accession>
    <name>A0A1A0H4U5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">NADH-ubiquinone oxidoreductase B18 subunit (CI-B18)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_47242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709505.1">
        <property type="nucleotide sequence ID" value="XM_018858138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4U5"/>
    <dbReference type="STRING" id="869754.A0A1A0H4U5"/>
    <dbReference type="GeneID" id="30031114"/>
    <dbReference type="OrthoDB" id="268414at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008698">
        <property type="entry name" value="NDUB7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05676">
        <property type="entry name" value="NDUF_B7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0830">Ubiquinone</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="84" mass="9998" checksum="C682BE52D4AA4CF9" modified="2016-10-05" version="1">MALTEFPPLLSEQDFQKYKVPLKWRDRCAAYFALFQTCLIRQSANSSIDCHHDKHIWEECEALDLSRRKQELKEVKEKLRSNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X0</accession>
    <name>A0A1A0H4X0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709530.1">
        <property type="nucleotide sequence ID" value="XM_018856355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X0"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X0"/>
    <dbReference type="GeneID" id="30029331"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="69" mass="8133" checksum="CA2F35C9D8BFEFC8" modified="2016-10-05" version="1">MAQASQNKLWNLYYHHFPFYMLTGPEGAFLHLFIISFLSFVVYGLYSVIPTYVPFLISRSYYYLTGGDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X3</accession>
    <name>A0A1A0H4X3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709621.1">
        <property type="nucleotide sequence ID" value="XM_018856382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X3"/>
    <dbReference type="GeneID" id="30029358"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000386">
        <property type="term" value="F:second spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000389">
        <property type="term" value="P:mRNA 3'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000348">
        <property type="term" value="P:mRNA branch site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="292"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="51264" checksum="5E563CCF49AFA1FC" modified="2016-10-05" version="1">MNLEDYTSETESVSETDVYASPNHFSTVSSDLYLRPAFVATTVVRNTFGGTFQKAVPTPRIAEKRRQETKRPKKKRKKTDPGQPDFSGPWYGSSSSSDSSDEQDYKQERESEQLETPQAVEEEESETPITTELYTANDFMLSNINSSEERKTTYPRKLKLTLAGHAKGVTRLRYFPRLGHLLLSCGNDGKIFLWSLGGETSRKVRGYFGHSHAVKDVIFNSNGDKFLSCSYDNTIVLWETATGIILKRLSLSSTPNCAIFNPQNENEIIAGLANRRIEHYDFTSLHSPVQIYDHHLGSINSLTLITKEIFMSTSDDRTVRLWKWHINIPIKIIADPTQHSVPYAAVHPSESYIALQSMDDSIYVIHANGKYRYNKKKSFRGHKVAGYGIEVAFSNHGAYLMSGDARGQVNVWSWKKCDVVQKIRLGDGVISCLAAHPTESNVIAVAGRKGEIYICN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H510</accession>
    <name>A0A1A0H510_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_13792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709570.1">
        <property type="nucleotide sequence ID" value="XM_018854428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H510"/>
    <dbReference type="STRING" id="869754.A0A1A0H510"/>
    <dbReference type="GeneID" id="30027404"/>
    <dbReference type="OrthoDB" id="10251727at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="646" mass="73625" checksum="E6B28918FFC75AEE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H540</accession>
    <name>A0A1A0H540_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid desaturase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709726.1">
        <property type="nucleotide sequence ID" value="XM_018858111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H540"/>
    <dbReference type="STRING" id="869754.A0A1A0H540"/>
    <dbReference type="GeneID" id="30031087"/>
    <dbReference type="OrthoDB" id="1461976at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03507">
        <property type="entry name" value="Delta12-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012171">
        <property type="entry name" value="Fatty_acid_desaturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32100">
        <property type="entry name" value="OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid desaturase" evidence="2">
        <location>
            <begin position="105"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="416" mass="47710" checksum="0E30512F638DBA94" modified="2016-10-05" version="1">MSQTAPSVSSSTGASALATTVKKTGNVSTLSHNLTAIDTYGNEFQAPDYTIKDILSAIPAHCYERRTLESLYYVARDISCMWALGYVANTYIPVLPHAAFRFVAWTAYSIAQGLVGTGLWILAHECGHLAFSDSSLVNDTVGWVLHSWWLVPYFSWKFSHGKHHKATGHMTRDMAFVPNTRELFVEKRQALSLEEITSDTPLMSLYTLFLQQFGGWWAYLFCNVTGQPVSDTYWGMNHFNPASALFEKDQYWYIVLSDIGLLTQLFVVYTWYQNYGAFNVLVNWFLPYVGVNHWLVFLTYLQHTDPEMAHYESDQWNFARGAAATIDREFGFVGQYLFHDIIETHVLHHYCSRIPFYNAREASEAIKKVMGKHYRHTDENMFKSLWKSIRMCQFVEGDNGVMMYRNINGLGTPPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H629</accession>
    <name>A0A1A0H629_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sec39 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45887</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710014.1">
        <property type="nucleotide sequence ID" value="XM_018857970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H629"/>
    <dbReference type="STRING" id="869754.A0A1A0H629"/>
    <dbReference type="GeneID" id="30030946"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013244">
        <property type="entry name" value="Sec39_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787:SF3">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40787">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08314">
        <property type="entry name" value="Sec39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291673" description="Sec39 domain-containing protein" evidence="5">
        <location>
            <begin position="21"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="Sec39" evidence="6">
        <location>
            <begin position="51"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="830" mass="94706" checksum="85CAB2F8B6BFB868" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H658</accession>
    <name>A0A1A0H658_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_25395</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709924.1">
        <property type="nucleotide sequence ID" value="XM_018855294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H658"/>
    <dbReference type="STRING" id="869754.A0A1A0H658"/>
    <dbReference type="GeneID" id="30028270"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF3">
        <property type="entry name" value="FI21236P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="12"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="175"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="81"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="265" mass="29634" checksum="02ED4C6F2E62410C" modified="2016-10-05" version="1" fragment="single">QPTKMSGPVDPSRVYVGNVNFNATEEELQEFFQDFKVLSVEIPSKTITRGENSFEKRLGFGFVQFENENDAVNAISQMNGKKFKQRQIHATKALPPATEEEKQKKKDLALAKQAELKAKKREAAKQANQAKQAKEAQQAKEAKEKTGSTATTKKPSPGKSTENVSKTPTGKISTDTIFITNLDYKTNGKGLSDLFKDLKPIWVNVPTRRVPFHMLKRFNALKKPIFNKGIGFAKFADEETQLRAIQEFNGTEINGRKIIVEAAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Q3</accession>
    <name>A0A1A0H6Q3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_45507</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710231.1">
        <property type="nucleotide sequence ID" value="XM_018857923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Q3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Q3"/>
    <dbReference type="GeneID" id="30030899"/>
    <dbReference type="OrthoDB" id="5229455at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF43">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G01850)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00755">
        <property type="entry name" value="AFLATOXINBRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="621" mass="70046" checksum="66E09915EF78C3A0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U9</accession>
    <name>A0A1A0H6U9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ribose-phosphate diphosphokinase</fullName>
            <ecNumber evidence="2">2.7.6.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_44826</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose-phosphate pyrophosphokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710281.1">
        <property type="nucleotide sequence ID" value="XM_018857843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U9"/>
    <dbReference type="GeneID" id="30030819"/>
    <dbReference type="OrthoDB" id="413572at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000005">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002320">
        <property type="entry name" value="PRK01259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF48">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="11">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="320" mass="34823" checksum="DFFAFFE871AA7160" modified="2016-10-05" version="1">MLSNSIKLLASDVHRGLAELVAARLGLKISPSELKRDSTGEVTFSIGESVRDEDIFIICQIGSGVVNDRVLELLIMINACKTASARRITVILPNFPYARQDRKDKSRAPITAKLMADMLTTAGCDHVITMDLHASQIQGFFDVPVDNLYAEPSVVRYIREKVSFKDAIIISPDAGGAKRAAGLADRLDLNFALIHKERARANEVSRMVLVGDVTDKICIIVDDMADTCGTLAKAAEVLLDNNAKEVIAIVTHGILSGNAIKNINNSKLKAIVCTNTVPFEEKLELCPKLDTIDVSGVLAESIRRLHNGESISYLFKNAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H8</accession>
    <name>A0A1A0H7H8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pet127-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_45233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710506.1">
        <property type="nucleotide sequence ID" value="XM_018857896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7H8"/>
    <dbReference type="GeneID" id="30030872"/>
    <dbReference type="OrthoDB" id="10249045at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000964">
        <property type="term" value="P:mitochondrial RNA 5'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013943">
        <property type="entry name" value="Pet127"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31014:SF0">
        <property type="entry name" value="MITOCHONDRIAL TRANSLATION SYSTEM COMPONENT PET127-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08634">
        <property type="entry name" value="Pet127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="623" mass="72023" checksum="BD2E9ACE8E6292D8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K4</accession>
    <name>A0A1A0H7K4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">NADH-cytochrome b5 reductase</fullName>
            <ecNumber evidence="14">1.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_32972</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="14"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710485.1">
        <property type="nucleotide sequence ID" value="XM_018856178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K4"/>
    <dbReference type="GeneID" id="30029154"/>
    <dbReference type="OrthoDB" id="432685at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090524">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NADH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000069">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000009">
        <property type="entry name" value="NADH-cytochrome b5 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001834">
        <property type="entry name" value="CBR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370">
        <property type="entry name" value="NADH-CYTOCHROME B5 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19370:SF143">
        <property type="entry name" value="PLASMA MEMBRANE-ASSOCIATED COENZYME Q6 REDUCTASE PGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00406">
        <property type="entry name" value="CYTB5RDTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13 14" id="KW-0274">FAD</keyword>
    <keyword evidence="4 13 14" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10 14" id="KW-0520">NAD</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="25"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601834-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="267" mass="30479" checksum="793B7BB20149283D" modified="2016-10-05" version="1">MTFTAIICTINFWEAWRRRKSIDPVKWNDLELIDKTVISKNSAIYRFKLFHEDEVLDIPAGHHVACCFSIDGKDEIRYYSPISNRFDTGFFDILVKSYPNGKVSKRFAMLKEGQTVKFRGPVGRLNYTTNMADHIGLVAGGSGITPILQVITKVITTPEDKTKLTLLFGNETENDILLQNELDEIAKKYPDFSVHYAVTEPSAAWEGETGYITKEMIEKYMPKPSHENRLFICGPPEMKKSLIEASSELGWEKTTMKSLPEDQVFCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7N6</accession>
    <name>A0A1A0H7N6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Glucose-6-phosphate 1-epimerase</fullName>
            <ecNumber evidence="3 5">5.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_32993</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the interconversion between the alpha and beta anomers from at least three hexose 6-phosphate sugars (Glc6P, Gal6P, and Man6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-glucose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:16249"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="ChEBI" id="CHEBI:58247"/>
            <dbReference type="EC" id="5.1.3.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glucose-6-phosphate 1-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.15" evidence="3 5"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710520.1">
        <property type="nucleotide sequence ID" value="XM_018856185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7N6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7N6"/>
    <dbReference type="GeneID" id="30029161"/>
    <dbReference type="OrthoDB" id="1659429at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09020">
        <property type="entry name" value="D-hex-6-P-epi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008183">
        <property type="entry name" value="Aldose_1/G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025532">
        <property type="entry name" value="G6P_1-epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122">
        <property type="entry name" value="APOSPORY-ASSOCIATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11122:SF13">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016020">
        <property type="entry name" value="PHexose_mutarotase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="6">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016020-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="304" mass="34078" checksum="057D0DE6629C9FCA" modified="2016-10-05" version="1">MPVHQTDDKVILTYPGNSDTKVEILKFGATVVSWISDKKERLWLSDAAKLDGSKAVRGGIPLVFPVFGKSKDSSHPTASLPQHGFARNSEWEFLGQVTENPVSVQFGLGPENVSPELYKLWGTGTYDFTLVLTVTLAENNLTTEIEVENSGKEPFEFNWLFHTYYNVDDVTDTLATNLIDTECYDQLLALTYKEKAPMLSFHEEFDRIYKNIDGDRDVQLIDKGNVVVTVSRKNLPDAVVWNPWIKKSEGMGDFEPKSGYLNMLCVEPGHVADFVKLNKGDKWIAAQSMTLNGEIKVQSDIFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7W0</accession>
    <name>A0A1A0H7W0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Autophagy-related protein 101</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43432</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG101 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710706.1">
        <property type="nucleotide sequence ID" value="XM_018857655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7W0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7W0"/>
    <dbReference type="GeneID" id="30030631"/>
    <dbReference type="OrthoDB" id="10259639at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012445">
        <property type="entry name" value="ATG101"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13292:SF0">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07855">
        <property type="entry name" value="ATG101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="222" mass="25096" checksum="B0EB4ACF3AE88A4A" modified="2016-10-05" version="1">MEFSLNLTAERSVVRESLKGVLWTIFFHRLFGPVVPVTNEFLDVPYPMVAGAADIDSTMDQKIDELIKQDFSLATHRSQPSVGQIVVLFAQKVVAKSKKRTGWFGQVKEEPQAHTLRWESWVININCLPLLYREHQQLAAASSSVNSAEKLLRLSALSFEATVGQVMELANLHKDHIPPIMTLDVCPFPYDITIDPQSAPRRPPAADDDTWTHYIKKLMEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Z8</accession>
    <name>A0A1A0H7Z8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_211425</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAK10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710542.1">
        <property type="nucleotide sequence ID" value="XM_018855039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Z8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Z8"/>
    <dbReference type="GeneID" id="30028015"/>
    <dbReference type="OrthoDB" id="269405at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057983">
        <property type="entry name" value="NAA35-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007244">
        <property type="entry name" value="Naa35_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057982">
        <property type="entry name" value="TPR_NAA35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373">
        <property type="entry name" value="GLUCOSE REPRESSIBLE PROTEIN MAK10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21373:SF0">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 35, NATC AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04112">
        <property type="entry name" value="Mak10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25789">
        <property type="entry name" value="TPR_NAA35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like N-terminal" evidence="5">
        <location>
            <begin position="28"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="NAA35-like TPR repeats" evidence="6">
        <location>
            <begin position="323"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="768" mass="87311" checksum="D36B6821617A870F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C5</accession>
    <name>A0A1A0H8C5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="5 6">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_43256</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the IPP transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="5 6"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710886.1">
        <property type="nucleotide sequence ID" value="XM_018857633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8C5"/>
    <dbReference type="GeneID" id="30030609"/>
    <dbReference type="OrthoDB" id="775260at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030666">
        <property type="entry name" value="IPP_transferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF89">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039110">
        <property type="entry name" value="IPP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR039110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="54044" checksum="1B71274E65410146" modified="2016-10-05" version="1">MILRHLRYIKNLITTKKMKNVVTIVGTTGVGKSQFSIELAKSINGEIINADSMQVYRGAPIISNKHPLDEREGIAHHVMDHIPWNEEYFIHRYSAEAVSAIDDIHARGKTPIIIGGTHYYLQNLLFKNKTIGEKEEKEKLIPLTQEQQDLLDGPVEAIFEALTNVDPVISEKFHPKDTRKLRRALEIYYTTGQKPSEMYHEQKLDELEDTSLKYNTLLFWIYCDPEILKLRLDQRVDSMMGAGALDEIRELNQFYESQTLPPDMTKGIWQVIGYKEFRPWLTGGQTDTKLFEEGVERMKIRTRQYAKYQVKWIRKLLGVELNKESRFGFKYGGKMYLLDATDLSKWATDVRARGLSIVDQFIAKGPLGVTEAQAPEKLREIFPSSEFYEEFNSNKTIKAVENWKHFECPVCKDSQGSPLVAVGEDNWSIHQKSRRHKKQISYNQNKRKHEELLKKYKKVKEDILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8H0</accession>
    <name>A0A1A0H8H0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_44357</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710704.1">
        <property type="nucleotide sequence ID" value="XM_018857783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8H0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8H0"/>
    <dbReference type="GeneID" id="30030759"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015916">
        <property type="term" value="P:fatty-acyl-CoA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042758">
        <property type="term" value="P:long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="25"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="436"/>
            <end position="664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="712" mass="80376" checksum="B929F24656F71460" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8L9</accession>
    <name>A0A1A0H8L9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycosyltransferase family 34 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_44326</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710862.1">
        <property type="nucleotide sequence ID" value="XM_018857776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8L9"/>
    <dbReference type="STRING" id="869754.A0A1A0H8L9"/>
    <dbReference type="GeneID" id="30030752"/>
    <dbReference type="OrthoDB" id="205108at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008630">
        <property type="entry name" value="Glyco_trans_34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31306:SF10">
        <property type="entry name" value="ALPHA-1,6-MANNOSYLTRANSFERASE MNN11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05637">
        <property type="entry name" value="Glyco_transf_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="420" mass="47910" checksum="751A3F3B16E04D9F" modified="2016-10-05" version="1">MRFRPKSTGAAPAGHLPMPGGFHRIKAVRANRLWLVVFALFFLWVFRPLRWIPSFSRHHPVYPPGLPPGCKHTVSSASPYIYPPIEDADLLRQIGVTNLIHITPEDAGRPLRMESLSSLDDADPVKQKIKEDNENAASNEARAINHFKNQAKVVFSPKLAKKSPKIVIVTALDFDRYPTASLAKLVQNRVNYAHEHGYGIYVRWAQEFVPELNSMDFLGDVERSKWIRLFCVRAAMFAFPDAEWFWYFDQDGLVTNMKVDLYSYLLTPDAWRGANLKEQPVIPPRGLIKTYKNLRPANVDLIFTQSETKIETNSFLVRNSEIGRATIETWRARHYLEYNNFPYGPDSAITHILQWHPFVLSKTSIVSARTINSFFAETVSLEDKATDHMHYFPGDFVVQWASCGTSTECDLILQKVAGDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H918</accession>
    <name>A0A1A0H918_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_44285</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711141.1">
        <property type="nucleotide sequence ID" value="XM_018857771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H918"/>
    <dbReference type="STRING" id="869754.A0A1A0H918"/>
    <dbReference type="GeneID" id="30030747"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="293" mass="33579" checksum="1A1B9618D3DF2F34" modified="2016-10-05" version="1">MICVECTNPEITCLHSEYKSKYVQLTVCPRCGNFADRYIEFDNVILFLDLVLLRPQAYRHVAFNVVEQTLFAGAQPPGVFRRYRKLARFFLLSVLFEVYLKWAYEEKSTPHTLLKARVLAWLPVWQYSFFVCQQMAEKTVFFSLVLVLFMWASGSAEVVNSNLQPPFRRAYYLCVLILTLLMSLAVKCLPILMLIWPYDNATVASMAVDVLSVFNTIEALHINTRCSYATTICIIAVSMLVLMVAKQLATSHFVAFLSPDYTWLQLFKHECLSFQKEVCAMAAAFSPVAWGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H938</accession>
    <name>A0A1A0H938_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_200338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711037.1">
        <property type="nucleotide sequence ID" value="XM_018854923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H938"/>
    <dbReference type="STRING" id="869754.A0A1A0H938"/>
    <dbReference type="GeneID" id="30027899"/>
    <dbReference type="OrthoDB" id="75720at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="28006" checksum="42355E99B45820A1" modified="2016-10-05" version="1">MQTFLFITFDSLQIISSIAILRQIHTRRSIYALSIDFTLYSWVWAFTHTIANCLYSFLPNVYAQYAKRYPLSPEIPHSTLLTAMCFFQLILCTILVNKCFFVFKSQEKLSVVCKTVMLLCISVFLRFSWLFCNGKATINVLDLADCLHNIGSVALACRLIPQISLNWFLEIFLLSNTFLTLQLCAATSGIVSLFIAYSSGLLWSEMPIGLPCQAVLGATWCASLVLLFQRSHYGQKHNIHRFQNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H958</accession>
    <name>A0A1A0H958_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711057.1">
        <property type="nucleotide sequence ID" value="XM_018856068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H958"/>
    <dbReference type="STRING" id="869754.A0A1A0H958"/>
    <dbReference type="GeneID" id="30029044"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF8">
        <property type="entry name" value="NUCLEAR MRNA EXPORT PROTEIN THP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="55119" checksum="E83E376EC0E5844E" modified="2016-10-05" version="1">MECVEDFLQRLQNCTCLDLRQQPLKLLELLRLHPQMNPFVVKINNLGHLSEGQILLLIERRHWFNGAWLSFDQLIVSFLRLCNQVNPWSLLESFDLYTTYLNDITVAFMNKKHGYLITYLLEDVLKQVLYMSKLLDYHMLHREMCRKPRLNFMASILLKIFNNIRSQIGADDYIDAVKKSIMVLIGVKLCQTYFQLSNPLLCQNVFSNMGNANLDFSSYHPNQQLQYRFYLAKFYIAKYEFVDAFEHLDWCLARVPYNYSVDSMNVSTILRQFLLVSILLGKRPKFGSFSRRYFSAPLKCPKYFSIYAELENAIRLGNFYRLHNLINDAEISSFLKRQKFASFFSSKAYLITLRNLVKTIWLSQGKQVRLGYDATKQGLLLSLNGLELSSVSTMNSSPKQHILSTNLDDHSIENCLVTLIDQNLLRGKVFPRLRVVSLAKNEVFPDISANYFLKFGNGSGDTLRPADQWIKRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9H3</accession>
    <name>A0A1A0H9H3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_16498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711059.1">
        <property type="nucleotide sequence ID" value="XM_018854651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9H3"/>
    <dbReference type="GeneID" id="30027627"/>
    <dbReference type="OrthoDB" id="4092739at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="373" mass="43335" checksum="1E46E0AB26B1BF08" modified="2016-10-05" version="1" fragment="single">SLDSSINTMERILSILHNAHSTKMETRDSVLKLYDSAPEAFESKNMALLGENIFDLKTIDILIDLQLGSNVDIFKRSNPSHARNILQKYISAFKDQYARDSSYLCMESLGRSYAENTRKANATLNNAIRRTASKAMIGESILHDEPPIHLDFCRLNTYNPFRNDIMDPFALQKGLSTKKHPANLFGAMINDQRVLLSFLHNLKKRISPLTIQNIMVAQSMFGNLALKSVVKRRLLSTDPRMPLDTPFEFYHLDITDENNKLKEFKAIVVYRSMILNRLEWFPPFRKSLAELEENKYDSMEKIIAIMADTFDRFFGLCFKTDAKYCDKWVENLMTFYTRNKSNEIFFQHLLDDAVVYFKEKVSQLSFETYSSKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9L8</accession>
    <name>A0A1A0H9L8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carboxypeptidase</fullName>
            <ecNumber evidence="13">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_32558</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in degradation of small peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711104.1">
        <property type="nucleotide sequence ID" value="XM_018856083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9L8"/>
    <dbReference type="STRING" id="869754.A0A1A0H9L8"/>
    <dbReference type="GeneID" id="30029059"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008442">
        <property type="entry name" value="Propeptide_carboxypepY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05388">
        <property type="entry name" value="Carbpep_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444431" description="Carboxypeptidase" evidence="13">
        <location>
            <begin position="19"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Propeptide carboxypeptidase Y" evidence="14">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA20582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="545" mass="61685" checksum="AB47CAC89F424E69" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9V1</accession>
    <name>A0A1A0H9V1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711297.1">
        <property type="nucleotide sequence ID" value="XM_018858993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9V1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9V1"/>
    <dbReference type="GeneID" id="30031969"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="31"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="33922" checksum="4C323F135F1799C7" modified="2016-10-05" version="1">MWIRAYATAARDTVNHGKHGLHPWPKTKKPTPHEIFNLLEKDCLNRLAYDRAVRRTYQKLVKLYHPDISKLREIENTDGSVLLEDQKKKRFHEVQSAYEILKSARTRTAYHRAQTTTWGDYKRGKTSSFDAYRMANAHRKKYAYENDPKFWQAGNWEDYYQMRYGRSAPTREEWEKNKWGILWKVLAAASVVVTLQVMLALEKTNEFNRQTRLMNLRANADLSDAYTNYDEGLTRFQRIRRFLLFRRLGLGDRDADGTKVAENEMLTKYAQEQLKRPELLEEYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9V4</accession>
    <name>A0A1A0H9V4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial inner membrane protease subunit</fullName>
            <ecNumber evidence="11">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_41855</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S26 family. IMP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711419.1">
        <property type="nucleotide sequence ID" value="XM_018857458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9V4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9V4"/>
    <dbReference type="GeneID" id="30030434"/>
    <dbReference type="OrthoDB" id="9996127at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.109.10:FF:000005">
        <property type="entry name" value="Mitochondrial inner membrane protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000223">
        <property type="entry name" value="Pept_S26A_signal_pept_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02227">
        <property type="entry name" value="sigpep_I_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00727">
        <property type="entry name" value="LEADERPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Peptidase S26" evidence="12">
        <location>
            <begin position="14"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600223-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="178" mass="19605" checksum="CA43470D0C6EC25C" modified="2016-10-05" version="1">MGFPVHPYLKRTLVAVTWLPVAFTVFDHVLLPCQVSGFSMAPTFNPGTESTAKDVVLIRKYNLKWPTSLERGDVVMFRSPEDPEKLVTKRVVGLQGDVIIPRDSLYPKKQALVPRNHLWVEGDNAFHLVDSNKFGPISQGLVVGKVMGIVWPLLRAGSDISGGARNGFLQGPETPESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA05</accession>
    <name>A0A1A0HA05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41293</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711356.1">
        <property type="nucleotide sequence ID" value="XM_018857379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA05"/>
    <dbReference type="STRING" id="869754.A0A1A0HA05"/>
    <dbReference type="GeneID" id="30030355"/>
    <dbReference type="OrthoDB" id="3980401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037504">
        <property type="entry name" value="PSI_induc_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40018">
        <property type="entry name" value="[PSI+] INDUCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40018:SF1">
        <property type="entry name" value="[PSI+] INDUCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="16376" checksum="6E179E4B3FAA694D" modified="2016-10-05" version="1">MPLDICPKSYAASLFKRDTLSTAESFKSWSTCMDNKTCKIVAIVGICVAALLVIWILATFVQCLCMGVSCLSALCCCCCRGAHKNRYVEPAQQPYENRNMYPRQPPMMQMEQTYQPVPHYGGYEENRYKKDDHEYTGYKPLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAA6</accession>
    <name>A0A1A0HAA6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_21128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711456.1">
        <property type="nucleotide sequence ID" value="XM_018855038.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAA6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAA6"/>
    <dbReference type="GeneID" id="30028014"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="235" mass="26082" checksum="64B025DDB25067E1" modified="2016-10-05" version="1" fragment="single">NIVVLIGGGHAAGKTTAAHSIKNDLQALPGHSANSLQIEVLDMQQYIDRTVTTTLSYAPNSAITVGLKSRILLKPSRFDFHALKTYISSRLARPDPTAPQKLIIVHGLYALYDKELCDMSLFKVYIDSDADTRLVRWIQRDVLPALGNGASLESVINLYLHGAKQEMADYIFQTKERADVIMPRGCEANGIALIVDGLLSYLGSDNVLEHKPINTLRPFAIERWDGDKQKFYELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAU3</accession>
    <name>A0A1A0HAU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41138</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711625.1">
        <property type="nucleotide sequence ID" value="XM_018857360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAU3"/>
    <dbReference type="GeneID" id="30030336"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903778">
        <property type="term" value="P:protein localization to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000488">
        <property type="entry name" value="Small monomeric GTPase (Gtr1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="330" mass="38032" checksum="931F3910E5ABED0F" modified="2016-10-05" version="1">MSQSSRKKLLLMGRSGSGKSSMRSIIFSNYSAFDTRRLGATIDVEHSHLRFLGNMTLNLWDCGGQDVFMENYFTAQKDHIFKMVQVLIHVFDVESKLINKDIEIFVKALTNLQKFSPDAKIFVLLHKMDLVQLDKRDELFHIMMDKLQKISNPFHFKLVGFPTSIWDESLYKAWSQIVCSLIPNMNLFNSNLLKFNEVLDAEEIILFEKTTFLVISSTASIQRQSQAQGSHLSNGSLDALDPKRFEKISNIIKTYKQSLGKLRSNFQNLVIRGSNGLHFYVDFLTDNLFIMIVLRDRGTDKSHRHALEDLLILENVKAARKWFEKIEAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAZ2</accession>
    <name>A0A1A0HAZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">6-O-methylguanine-DNA methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA repair MTase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41684</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711804.1">
        <property type="nucleotide sequence ID" value="XM_018857441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAZ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HAZ2"/>
    <dbReference type="GeneID" id="30030417"/>
    <dbReference type="OrthoDB" id="2548197at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032132">
        <property type="term" value="F:O6-alkylguanine-DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070911">
        <property type="term" value="P:global genome nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052520">
        <property type="entry name" value="ATL_DNA_repair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942">
        <property type="entry name" value="6-O-METHYLGUANINE DNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42942:SF1">
        <property type="entry name" value="ALKYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="5">
        <location>
            <begin position="9"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00030795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="119" mass="13351" checksum="7A558A1BFAF515C5" modified="2016-10-05" version="1">MLSDEAKAFHYAVFSAVSQIPEGRVTSYGHIAYLVGKPQNARQVGSSMKNYQTINAVLLRDGVSFEQLPWWRVIASSGQIAKREGELEQKRRLLLEKVEVNGMSVSMSVYGWFPDDVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB42</accession>
    <name>A0A1A0HB42_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF1771-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711618.1">
        <property type="nucleotide sequence ID" value="XM_018855899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB42"/>
    <dbReference type="STRING" id="869754.A0A1A0HB42"/>
    <dbReference type="GeneID" id="30028875"/>
    <dbReference type="OrthoDB" id="3231855at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013899">
        <property type="entry name" value="DUF1771"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053020">
        <property type="entry name" value="Smr_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47417:SF1">
        <property type="entry name" value="SMR DOMAIN-CONTAINING PROTEIN YPL199C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08590">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01162">
        <property type="entry name" value="DUF1771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="96"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="232" mass="26720" checksum="ACC31A465B379557" modified="2016-10-05" version="1">MTSVLERGAALFDERDYNHATDSTYKDYRQQASDLYEKKNKLSQQSQQAYQRGDKQKAHELSEQLKQVLQEAENCNKKAAEYVFIENNTDSKSDEIDLHGLYVKEAKWILQRRISECIRTNQPHIKVIVGKGLHSQNGVAKLKPAIDEMCDESRLKHYVDPHNAGVMVIELTNTSNLNIPSHWDSSPMGQGYQQQPQYQQQPQYQPQPQQQEFKTGNLLVDLLLKVVCNCLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBE0</accession>
    <name>A0A1A0HBE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ER membrane protein complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711815.1">
        <property type="nucleotide sequence ID" value="XM_018854110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBE0"/>
    <dbReference type="GeneID" id="30027086"/>
    <dbReference type="OrthoDB" id="1894652at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21203">
        <property type="entry name" value="ECM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012949645" description="ER membrane protein complex subunit 10" evidence="1">
        <location>
            <begin position="16"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="22917" checksum="52A98BE7B4A898E6" modified="2016-10-05" version="1">MLLFLLFSLASLVALQEFSIYTLNTVTSQESELGLLSYDPGQNSALFRPHLDLPSAKGSYSVCARDLACSAPCFAYQEIGENTPQGLGGKFKVAVGDDGSVTYISFERGEDPAHFACLVARVETAPTPNLNPFHRQAAQQNRKVQTQKVVRKKVVENDNGEKVEVDEETDEVVDVDSRSWVQKNWMYIVPPLVLFLIFSPAENKDGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBT2</accession>
    <name>A0A1A0HBT2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_180992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711858.1">
        <property type="nucleotide sequence ID" value="XM_018854770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBT2"/>
    <dbReference type="GeneID" id="30027746"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="259" mass="28700" checksum="21A9E5040F0111F0" modified="2016-10-05" version="1">MGPNGMFDGGLEVPVFSVLKVLVTRAAHQEAIQRNTAPHECTGLSSAQGEPPSDQKNGTGAAETTTRQTPGFEPFFSFLNPPERGRSMAFCWWAVAVACGRKARVAGAVCRRRHPHGLRLCIYFPFFSFYFFALQNFNTYSPSLLQLPVPSARFLCFHLSCFFLSIRSIALTPGLPLSPHRASQVPNQKAELWICPLISKHQHSRVLPQPSFPAQPQAPDLHPFSSPPLLSAAAQRSFFFSFFPDFSPPEAIRTVNEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBT6</accession>
    <name>A0A1A0HBT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_42047</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711955.1">
        <property type="nucleotide sequence ID" value="XM_018857488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBT6"/>
    <dbReference type="GeneID" id="30030464"/>
    <dbReference type="OrthoDB" id="4138121at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020301">
        <property type="entry name" value="Mrx7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10906">
        <property type="entry name" value="Mrx7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="80" mass="9778" checksum="682640616D8A6CDD" modified="2016-10-05" version="1">MRPPTSLEELLYRKLMTSHAFHSWVRRVHARINRIPLEEQTLEADNSIHLLNYRPTTWHKVNAFRIIFWDELKKSFTFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBY5</accession>
    <name>A0A1A0HBY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_41237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712159.1">
        <property type="nucleotide sequence ID" value="XM_018857372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBY5"/>
    <dbReference type="GeneID" id="30030348"/>
    <dbReference type="OrthoDB" id="421671at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025638">
        <property type="entry name" value="DUF4336"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835">
        <property type="entry name" value="YALI0C07656P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="29915" checksum="0271A008E11C995A" modified="2016-10-05" version="1">MYPSPLAVSSRKLTDNIVLSVTGFKRMGRVNFGARMALFRLQDSVVVWSAIPFSDDVNKALELLTGQAAGFNVTHLIVPDSEHTMAAASFKQEFPRMKIIAMEGVQLGKGVSPDYVVTSKYGNKKIDAETLREIGVQEPQILDNFEFVYLPSHGNKELVAYHKESKAVFEADMLFNLRTDVPMEQFSPATGFPENYYPFSGMSFLAKFLNADSKTGRFLFRKMVNTKASAEGLQAINAWDFDTLVMCHGNVIEKGGKAAFQKVFLSVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC69</accession>
    <name>A0A1A0HC69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SAC3/GANP/THP3 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_21061</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712116.1">
        <property type="nucleotide sequence ID" value="XM_018855034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC69"/>
    <dbReference type="STRING" id="869754.A0A1A0HC69"/>
    <dbReference type="GeneID" id="30028010"/>
    <dbReference type="OrthoDB" id="199574at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF4">
        <property type="entry name" value="LEUKOCYTE RECEPTOR CLUSTER MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="2">
        <location>
            <begin position="163"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="43728" checksum="4DB3C492AA245B99" modified="2016-10-05" version="1" fragment="single">NTQGASWPPLLQDFVNRSFEQSNALSAPQKAVFNEQIQTLIYAAARDKKIWTNDWIRQKLPVFDPAVPLALYQDVVGPGLGAHQTPAPPGYAEPHNQQKRQHPLPEKPTNFDSNERKRQRAARFQALASLPHPVQPSLAPAAGKSATIVGTLTALEKRYLRLTSDPDPALVRSKFMLGKCLLYVVDKYRSSGALYAYINDQIKAIRQDMTVQHITSGLAVRVYETHGRIAIENNDLGEFNQCQSQLKHLYAQRSHQNFYKNTYEFTCYRILYLLLTGNYADINIARLDIMDADSATVELELDPEFRIRRQCVYKALELADHVVLGNYHRFFALYKWFKGLGCMSGAFHLLDQFMANKQRVLSLKIMCKGFKKVPLALLESQLAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC75</accession>
    <name>A0A1A0HC75_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TMEM14-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41065</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712105.1">
        <property type="nucleotide sequence ID" value="XM_018857348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC75"/>
    <dbReference type="GeneID" id="30030324"/>
    <dbReference type="OrthoDB" id="5620at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="107" mass="11263" checksum="2C605608A848369B" modified="2016-10-05" version="1">MELTTYTLAGIYAVGGTMGYVLRGSVHSLLVGGAVSALYGYSGYLLKDNADWGLKLALGCSTSMLATGIGRVIVTRFAKPVPVVLLVLGGLSSTYYFGKYNEFQGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC81</accession>
    <name>A0A1A0HC81_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ER membrane protein complex subunit 7 beta-sandwich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_41541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712126.1">
        <property type="nucleotide sequence ID" value="XM_018857418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC81"/>
    <dbReference type="STRING" id="869754.A0A1A0HC81"/>
    <dbReference type="GeneID" id="30030394"/>
    <dbReference type="OrthoDB" id="4085072at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23809" checksum="0C36D0BF6617732B" modified="2016-10-05" version="1" fragment="single">PIVNGLNFQSRIEVRLTEIGDFRSVKEKTLPVNRDYAFEAPDLAAGEYELTINAYDFIVRAPRWRIFVDDTETRAFVDAIASESYNISSQQIIQRDSPLTVPISGFKSYYESTEGRLTEVLMNSPFGFIFRSKLYSLLFFGTLVIMAFPYVIPYVAPDFAEQYQKMQAEMNAPPQENKPMGEILSKAAPKNQAISEKYSNSGKRTRKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCE4</accession>
    <name>A0A1A0HCE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712088.1">
        <property type="nucleotide sequence ID" value="XM_018857538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCE4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCE4"/>
    <dbReference type="GeneID" id="30030514"/>
    <dbReference type="OrthoDB" id="4073795at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="2"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="40295" checksum="8E4DC6847A26E391" modified="2016-10-05" version="1">MPLSLADLPPEVLENIFAHLSRKDVKRLALTSRAYRASAAPYVMRQLRASWGQLVALQDFVGDPDLHPRGALQDLAGNPDIYPRAAHPRLYCERLSICEPNAYYEYQQDTFGKLVARAVFPRLKHVSVLSASLSHWLKYNTCAHIQSVQIENAAAAGGSGRKVFCLDHVRGFAALALLTLTRFHFDWLDLGPSHGPVFAGRLRDLALHDCTWQYPFNLVQFNAQHSLRTLAITYSHDHSFVLLERFVDFLIDPLPQHLASLRRLEVGFVDFRDTKKVLTLTLMATFLRVFPGLDHLYLHGWHTTRTYAHVILAQMTFDFPVDVDVEIEAPGPSPRGVSAAPRPPVRWKQRLYNRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCH0</accession>
    <name>A0A1A0HCH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_32164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ATX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712201.1">
        <property type="nucleotide sequence ID" value="XM_018855994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCH0"/>
    <dbReference type="GeneID" id="30028970"/>
    <dbReference type="OrthoDB" id="689350at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006825">
        <property type="term" value="P:copper ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051881">
        <property type="entry name" value="Copper_transport_ATOX1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46365:SF1">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN ATOX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="3" id="KW-0187">Copper transport</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="63" mass="6461" checksum="8886C2D669B89DD5" modified="2016-10-05" version="1">MTCAGCSNAVLKALGRLQGVTESKVDLASQTVDVTTEDSLDYETVLGAISKTGKKVNGGKTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCK2</accession>
    <name>A0A1A0HCK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_77396</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712330.1">
        <property type="nucleotide sequence ID" value="XM_018858690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCK2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCK2"/>
    <dbReference type="GeneID" id="30031666"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF37">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="784" mass="89307" checksum="044D3FCBC02CE039" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCR1</accession>
    <name>A0A1A0HCR1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_14494</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712192.1">
        <property type="nucleotide sequence ID" value="XM_018854509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCR1"/>
    <dbReference type="STRING" id="869754.A0A1A0HCR1"/>
    <dbReference type="GeneID" id="30027485"/>
    <dbReference type="OrthoDB" id="4489171at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055335">
        <property type="entry name" value="Ucp6/RUP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39597:SF1">
        <property type="entry name" value="UBA DOMAIN-CONTAINING PROTEIN RUP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="98"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="337"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="489" mass="55101" checksum="0F1CBDDE769C790E" modified="2016-10-05" version="1" fragment="single">MDKDSQIKMLQEMGFLNNQAMEALSASGNDLNKAIAYLFGEVDASSGAGTQLAPYDVESEQIGIADSVGISNPHDIPEFLTLFVNEEGNMAEAMKENQGPFYSSNSASSSSSSSLEEVEDDSLLEEPEEEYPENVKTEFTNVPFLLSKRNKARSWVPLILILTHSSIFAGPVLQLRSKLSFIRELQQIVYFVQNFDNSKRWYHDTDKLAKILENDGPCGSLQDEELILNIIDFLMRKEPALKPIFESMVQSLDEDISNNLTVLEIDSDSRGSTLYETLNELFWQKDFEFLGRIKYSSVAPIVTYQLLAADSNTSHVPFQLLEIMYPEIYSDKALGRVREEIHLIKRAQMEYQKLNRKLMDLNFFEGKKIDGLLLQTASALQENNPNASEDLRELANLLQELRFAELENQAQAKSNASPQRLALLESIICDLPDLRAYNLVGVIFSETKYFVRAGENWVDMDGGVTIDYASVANAVERRIGSQNITLVYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCU2</accession>
    <name>A0A1A0HCU2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.205</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2'-O-ribose RNA methyltransferase TRM7 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_41467</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Methylates the 2'-O-ribose of nucleotides at positions 32 and 34 of the tRNA anticodon loop of substrate tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>cytidine(32)/guanosine(34) in tRNA + 2 S-adenosyl-L-methionine = 2'-O-methylcytidine(32)/2'-O-methylguanosine(34) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42396"/>
            <dbReference type="Rhea" id="RHEA-COMP:10246"/>
            <dbReference type="Rhea" id="RHEA-COMP:10247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74445"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.205"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. TRM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.205" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712248.1">
        <property type="nucleotide sequence ID" value="XM_018857405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCU2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCU2"/>
    <dbReference type="GeneID" id="30030381"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106339">
        <property type="term" value="F:tRNA (cytidine(32)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106340">
        <property type="term" value="F:tRNA (guanosine(34)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106050">
        <property type="term" value="F:tRNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002130">
        <property type="term" value="P:wobble position ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000176">
        <property type="entry name" value="tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03162">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028590">
        <property type="entry name" value="RNA_methyltr_E_TRM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="21"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="301" mass="33493" checksum="12F62F09EF5EC810" modified="2016-10-05" version="1">MGKASKDKRDLYYRRAKEEGYRARSAFKLLQINEEFDIFSGVKRVVDLCAAPGSWSQVLSQELNKTPGEDNAKIVAVDLQPMVPIEGVTCLQADITHPKTLQKILDLFGGESADFVCSDGAPDVTGLHDLDEYIQAQLVLCALQLTCCILRPGGTFVAKIFRGRDIDLLYSQFGYLFDRVVCAKPRSSRGTSLEAFIVCTGYRPRPGWNPKLDATKSTEEFFEDADIAKSYIMKNMELPLDEERSIAKFVSCGDLKDGDSDATYTLNSSVEQRNLQPVQLPTAPPYKKALAMKRNGELVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD36</accession>
    <name>A0A1A0HD36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_144396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712490.1">
        <property type="nucleotide sequence ID" value="XM_018854504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD36"/>
    <dbReference type="GeneID" id="30027480"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="93" mass="10542" checksum="EEAB644AA0D06208" modified="2016-10-05" version="1">MLTLPNAWYGPISQPTPSGPQSLPRFRLQPAADALQHRMPPENKPKTNQKQTRKQTKNKPKTTKATKATKATKTTKTTRPTKPKETIYARPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDC5</accession>
    <name>A0A1A0HDC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_143574</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712471.1">
        <property type="nucleotide sequence ID" value="XM_018854499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDC5"/>
    <dbReference type="GeneID" id="30027475"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="134" mass="15108" checksum="D38281D46B07B968" modified="2016-10-05" version="1">MRAIRKRGVRGALFMTNSGCLCEAGCSGFLIHGYRDPNTIDAFKWGVNPWIIWGMTELPYCEQRRYITVDHSCEQKNREETKYTAVNQESFSDHTTGNATVSHEGASYVALKLMGAVQKQTSRRIMCHGSKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDI6</accession>
    <name>A0A1A0HDI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Endo-1,3-beta-glucanase btgC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Laminarinase btgC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_39915</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation. Active on laminarin and lichenan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712453.1">
        <property type="nucleotide sequence ID" value="XM_018857202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDI6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDI6"/>
    <dbReference type="GeneID" id="30030178"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF17">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BTGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="15">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6405" checksum="9FFBF674503F5744" modified="2016-10-05" version="1" fragment="single">WPTAGDTNGDAVAGTAEQAAALSDIADKVGDHVLYFNAHNDGWKDPGYLSCEQYWGIFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDP3</accession>
    <name>A0A1A0HDP3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DUF367-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_23741</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712632.1">
        <property type="nucleotide sequence ID" value="XM_018855267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDP3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDP3"/>
    <dbReference type="GeneID" id="30028243"/>
    <dbReference type="OrthoDB" id="10262062at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="8">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="7">
        <location>
            <begin position="87"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="223"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="319" mass="35728" checksum="76BFF700B45BE9A0" modified="2016-10-05" version="1" fragment="single">MGKGKNKPSEERAQKGRTSNGHKLKQHHVRRGRQEEGVVVRTDTDKFPVKLAMWDFDHCDPKRCLGKKLERLGYIRNLRVGMKFQGIVVSPNGTGVVCPDDREIVEQQGAAVVECSWARLDEVPFNKIGGKHERLLPYLVAANTVNYGRPWKLNCVEALAACFAIVGREGLAAELLQNFSWGPTFLDINRELLRVYQQCTDLALVERAQDEWMAKLEDEVRQRKQQSAEGDVWMMGNVNRQPMDGSDEGSADESADEDEDGDDGPGPDVRYDNLGNIIADSASEESEDASEDEPEDEYTAELDGDDVVYDKLGNIVSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDQ7</accession>
    <name>A0A1A0HDQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_39212</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712554.1">
        <property type="nucleotide sequence ID" value="XM_018857115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDQ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDQ7"/>
    <dbReference type="GeneID" id="30030091"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030668">
        <property type="entry name" value="Spermi_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000502">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="12"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="296" mass="33653" checksum="9C6B984C1EE47811" modified="2016-10-05" version="1">MAQELTHPTIKDGWFAEVSDTMWPGSAMSLKVEKILYSQRSKYQDVLVFKSTNWGNVLVLDNCIQATERDEYSYQEMITHLALNSHPNPKKALVIGGGDGGVLREILKHESVEEAWLCDIDEDVIKVSKQYLPEMSKSYENPRTKVHIGDGFKFLADYQNTFDVIITDSLDPEGPAESLFQKPYFELLKGALTEKGVITTQAESIWLHMDIISKLKQDCKQIFPVAEYAYTSIPTYPSGTIGFMVCSKDGSAEVRKPLRKFSEDFEADNLRYYNSKVHEASFVLPQWADKILNKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDR8</accession>
    <name>A0A1A0HDR8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_10963</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712564.1">
        <property type="nucleotide sequence ID" value="XM_018853888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDR8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDR8"/>
    <dbReference type="GeneID" id="30026864"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052239">
        <property type="entry name" value="Ser/Thr-specific_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45998:SF2">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="29"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="371" mass="39411" checksum="CA90292681A4F9CF" modified="2016-10-05" version="1">MDLLLAAARCVPCAPVFGPPAVALNGHTYSVLRLLGEGGFSYVYLVAPRDDPAQRYALKTLRCPYGRDDATFQAAALEVQNYLRFAAVGSPHIIRPVAEAVVAEPGGASAFHVLLPYYERSLQDVLAAHVLGGSAMAPPDVARVFVGILRGVQRMHRYRRPAGAVPEPAPGTEASPMLGVAAATAATELAELAEECPYAHRDLKPANVMLAADGRPVLADLGSCRPARVHVALRQQALALADYAQEHCTLAYRAPELLDVALDACISEASDVWSLGCVLYACCFGLLPFERMERDQGASLRLAVSQGRYEIPRDTRGLLPQLLAVIRLCLVVDPARRPSVDVLLERCLLLPESAAASAAETVAGDGPGGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDU5</accession>
    <name>A0A1A0HDU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Complex 1 LYR protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712760.1">
        <property type="nucleotide sequence ID" value="XM_018857259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDU5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDU5"/>
    <dbReference type="GeneID" id="30030235"/>
    <dbReference type="OrthoDB" id="74240at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008011">
        <property type="entry name" value="Complex1_LYR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05347">
        <property type="entry name" value="Complex1_LYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex 1 LYR protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="95" mass="11222" checksum="63A21F7CFA5A4B29" modified="2016-10-05" version="1">MAVRFTQLSRFYSLKTKNILSLEEFIFRQNVLSTYRSLMRIIYKHHERAGLAQYAREEFRMNAKETELTTRKYLLQTGIAKVNDMANVMGINAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDX5</accession>
    <name>A0A1A0HDX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MATE efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77593</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712604.1">
        <property type="nucleotide sequence ID" value="XM_018858728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDX5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDX5"/>
    <dbReference type="GeneID" id="30031704"/>
    <dbReference type="OrthoDB" id="2126698at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13132">
        <property type="entry name" value="MATE_eukaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045069">
        <property type="entry name" value="MATE_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11206">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="338"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="489"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="516"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="576" mass="63480" checksum="F405AA4F71919440" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEA3</accession>
    <name>A0A1A0HEA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Complex III subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_39158</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The complex plays an important role in the uptake of multiple carbon sources present in different host niches.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein, 2 core protein subunits, and additional low-molecular weight protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UQCR10/QCR9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712729.1">
        <property type="nucleotide sequence ID" value="XM_018857104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEA3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEA3"/>
    <dbReference type="GeneID" id="30030080"/>
    <dbReference type="OrthoDB" id="44067at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.260:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.260">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008027">
        <property type="entry name" value="QCR9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036656">
        <property type="entry name" value="QCR9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12980">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX, SUBUNIT X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05365">
        <property type="entry name" value="UCR_UQCRX_QCR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81514">
        <property type="entry name" value="Subunit X (non-heme 7 kDa protein) of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="62" mass="7056" checksum="837C3B98F67E05FB" modified="2016-10-05" version="1" fragment="single">LSALIKRNSVYVSTIFAGAFAFQAFLDVGVTKWYENHNRGKLWKDVKGKFLEGGEEDDDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB7</accession>
    <name>A0A1A0HEB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Metal homeostatis protein BSD2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_25035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712832.1">
        <property type="nucleotide sequence ID" value="XM_018855285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEB7"/>
    <dbReference type="GeneID" id="30028261"/>
    <dbReference type="OrthoDB" id="10003116at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019325">
        <property type="entry name" value="NEDD4/Bsd2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396:SF5">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13396">
        <property type="entry name" value="NEDD4 FAMILY INTERACTING PROTEIN 1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10176">
        <property type="entry name" value="NEDD4_Bsd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="30734" checksum="A7AA26D492E39543" modified="2016-10-05" version="1" fragment="single">EGASAADLERQGAAQPGRLSAASRLKNVVNYLFPFKQTYERLNNGISTGRLQLNAPGRFVGQGTDGVFRNLMAKPDTEENRMEQEQHPPTYDEAAADSSPEYWEATMISPMYEDEVFVHGLPVGNIANFVWNVLVSVAFQLVGFMLCYLLHTSHASKQGTRAGLGITILMYGYNVIPANFGRPDKVPTRYEPSDPNLFDISKSLPIATGGAVDNYLLGFVQHLDTNETQSGAQTPVSAYGIMALGALILLKSLVDFYFIKREEKRILAPRALEEVHTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEJ9</accession>
    <name>A0A1A0HEJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_40706</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712823.1">
        <property type="nucleotide sequence ID" value="XM_018857303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEJ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HEJ9"/>
    <dbReference type="GeneID" id="30030279"/>
    <dbReference type="OrthoDB" id="3981028at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="200"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="346" mass="39355" checksum="0C93ABF87BF5A4EE" modified="2016-10-05" version="1">MDSYLLESSAPEHVMADDYQLALKIFMNKDIVRSFGMVSKLQPAAFRNLDRGLISEELFLKITTLYLTEVGLLLDPQVVPEASLNIQDRQQLVESLQKHVVVDQMAHLYGALADAPLNLLFHIFLLYYTSRDALQAGTDGSVLQQFSDTYHALEFQGRQEDRFLKRWFDMYVLNVLPDAGDFATAFAIAESNTVFGGALAVAKLKEVQRLKTQQEQARKKAQKELHAREARARAAEQEQERIDARERSLKYRSMQQIRAEQQSQEPPSSAGGPGDAPAAALTLQRIRERLRCYYVLSKNGVKTHSPVLLAVVVLLLICGLLLRRRKINFRQRVKETLRMAFKVTYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEW0</accession>
    <name>A0A1A0HEW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_155156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713032.1">
        <property type="nucleotide sequence ID" value="XM_018854605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEW0"/>
    <dbReference type="GeneID" id="30027581"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="115" mass="13669" checksum="7A9231D329BA0B27" modified="2016-10-05" version="1">MYIFLVVRNLPPSNIVFHRLVICVYTFCAPLSCTPYYFSKRTVLVYFVLVCFLKTRCNHLSLSIQSYRVLQAISKIMRSSRIHLLPFQKQCKHRTTRRQRNRSSGIVIQGYDRYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFE2</accession>
    <name>A0A1A0HFE2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_39457</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713113.1">
        <property type="nucleotide sequence ID" value="XM_018857145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFE2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFE2"/>
    <dbReference type="GeneID" id="30030121"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="182"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="418"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="569" mass="62396" checksum="5D3F9B41FD357121" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFG4</accession>
    <name>A0A1A0HFG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713370.1">
        <property type="nucleotide sequence ID" value="XM_018859341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFG4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFG4"/>
    <dbReference type="GeneID" id="30032316"/>
    <dbReference type="OrthoDB" id="429932at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012578">
        <property type="entry name" value="Nucl_pore_cmplx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28003:SF1">
        <property type="entry name" value="NUCLEOPORIN POM34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08058">
        <property type="entry name" value="NPCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="379" mass="42999" checksum="387EBBAD3CCF3D02" modified="2016-10-05" version="1">MPLSTGVRSFFAHSDAKGNSTGVEDENHASSLKDMTGSDVNEAGNNFFLKRASNFFDLISHEFEPLPSEVNQVSPEASNKNTKKYGDLSSSDELFAGGTPQPNTHHSQYIRPLIELGSVISNDYLPDSLHRQSRNPIIAYEESGKEKKKVNNDEYEDLSDDELDKEAVPTGEWTSPVVREALARQVSKELQFKTLWTNILRLFSAHFFFVVTQKVVQIYWASHGLEASPDNAFLIKFFQPHHVEYAVTLVGKAHEYARHLLWLFVVIIIFDIAMVLWPQDQCKDLPLTVKQRELIGLKSIPSGETGDVSDTASLEISQRLFESASTKPLHIPKYSRINELPNLIKRKEKDNFMEELASDLKHVKHQNRLNHASRIIEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFL8</accession>
    <name>A0A1A0HFL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_85189</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713277.1">
        <property type="nucleotide sequence ID" value="XM_018859244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFL8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFL8"/>
    <dbReference type="GeneID" id="30032219"/>
    <dbReference type="OrthoDB" id="423313at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005796">
        <property type="term" value="C:Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF5">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508830" description="Glutaredoxin domain-containing protein" evidence="2">
        <location>
            <begin position="27"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="3">
        <location>
            <begin position="106"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="211" mass="23054" checksum="3F751AE8ED69FD38" modified="2016-10-05" version="1">MANGKKSKVLALALALLLVFVILIRGRGAYDWASAPAGTVPAAKSLLDSQNNEVVDNAINQEISREKIKEETGGGAGENTDSGDVEPLDYDPSKEFLQIRALAPMTIFSKTYCPYLKQLKKLLLENYSITPAPQIVELDKHKHGRELQEYVGHVSERNTVPNVIIGTELTLSKGGASEFTKLHKEGKLVEMLKLWGGKAISVLRLEVPSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY1</accession>
    <name>A0A1A0HFY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_228138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713244.1">
        <property type="nucleotide sequence ID" value="XM_018855195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY1"/>
    <dbReference type="GeneID" id="30028171"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="140" mass="15864" checksum="82C1F9A668A0B120" modified="2016-10-05" version="1">MYSKHKDTGKSKTDKLEILDSMLNDKESFRNGERNNLPSGESEFKLLVEKGAKEYTNVLRVQENHMNYIAEKAKAEKPNFETVEMINGTKASKPHPDVTIDADSTLVQVARFHVSKMVCPCRSRQELITMIGIAHSSNHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG26</accession>
    <name>A0A1A0HG26_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_35276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713289.1">
        <property type="nucleotide sequence ID" value="XM_018856591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG26"/>
    <dbReference type="GeneID" id="30029567"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291786" evidence="1">
        <location>
            <begin position="23"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="96" mass="10442" checksum="B096DA5B9C646DDA" modified="2016-10-05" version="1" fragment="single">KKIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPTFVIEETSDSVNVKLLGSCITFYCASFVDLNSNTLQIVEMLAALHLKGTYLGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG30</accession>
    <name>A0A1A0HG30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pre-mRNA-splicing factor CWC26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_36937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CWC26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713333.1">
        <property type="nucleotide sequence ID" value="XM_018856803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG30"/>
    <dbReference type="STRING" id="869754.A0A1A0HG30"/>
    <dbReference type="GeneID" id="30029779"/>
    <dbReference type="OrthoDB" id="6022at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070274">
        <property type="term" value="C:RES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018609">
        <property type="entry name" value="Bud13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051112">
        <property type="entry name" value="CWC26_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31809:SF0">
        <property type="entry name" value="BUD13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09736">
        <property type="entry name" value="Bud13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="263" mass="29824" checksum="996D58F0D188C6D8" modified="2016-10-05" version="1">MASRADYLSKYLSKDDSKKAKKKSKKAGGAIAEANSVKKETTLIKDTIPEPESEFEPTSVDLGSSLAVKGGFKRIDNGQIVKNETPSSEMPKEENLPATVYRDLSGRIIDLKEKRAEIKARKEEEAKREEIEKEQINTGELDRIRQDEEAKKLAKSTRFDYSKTSKEYTDYMGQKDRFEDPLAGRLATSALNPEVSVSATNRPVYRGGQHPVNRFKIQAGHFWDGIDRGNGFEERLVKARDLIYVQRVTDQAAKETYTEYDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG36</accession>
    <name>A0A1A0HG36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_29395</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713299.1">
        <property type="nucleotide sequence ID" value="XM_018855393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG36"/>
    <dbReference type="STRING" id="869754.A0A1A0HG36"/>
    <dbReference type="GeneID" id="30028369"/>
    <dbReference type="OrthoDB" id="205099at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055122">
        <property type="entry name" value="Med14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013947">
        <property type="entry name" value="Mediator_Med14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809">
        <property type="entry name" value="MEDIATOR COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12809:SF2">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08638">
        <property type="entry name" value="Med14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit MED14 N-terminal" evidence="11">
        <location>
            <begin position="42"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1023" mass="115683" checksum="FB7D1AD406BAFCB2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG47</accession>
    <name>A0A1A0HG47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome b560 subunit of succinate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_29661</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713615.1">
        <property type="nucleotide sequence ID" value="XM_018855454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG47"/>
    <dbReference type="STRING" id="869754.A0A1A0HG47"/>
    <dbReference type="GeneID" id="30028430"/>
    <dbReference type="OrthoDB" id="588261at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03499">
        <property type="entry name" value="SQR_TypeC_SdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018495">
        <property type="entry name" value="Succ_DH_cyt_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10978:SF5">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE CYTOCHROME B560 SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01001">
        <property type="entry name" value="SDH_CYT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="171" mass="18363" checksum="1BE2FC3AB67713A1" modified="2016-10-05" version="1">MLSRVGLRPLARFPLRSAAFSAVRASSTSTVKVSHDQEQELLVNQRKNRPVSPHLTIYTPQITMVLSGLNRITGVAMAGAFYALTCGYAATSLLSIPFDSAVLVSAFAGLPFAAKLAAKAAMSFPFVFHSLNGVRHLVWDFGKELTISGVNRTGYAVVAATAALGSWMIFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGA2</accession>
    <name>A0A1A0HGA2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_18625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713369.1">
        <property type="nucleotide sequence ID" value="XM_018854803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGA2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGA2"/>
    <dbReference type="GeneID" id="30027779"/>
    <dbReference type="OrthoDB" id="429932at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF47">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="188" mass="20841" checksum="FD9A551EA107DA49" modified="2016-10-05" version="1" fragment="single">MQSIHRLAPEDCKKIHSQVVIRSILDVIRELVHNSIDAGASCISIKINLESLSVCIEDNGTGISVSDMGQVGRRYYTSRASQVETSNTLPQELTNMMPPELTRSLGFKGESISSIATLCSKLVILSAVKNVVHSMVVDGENSSCLINLLLEFFMVKVPTQGSIIIVSRLFHNVPVRRKYHKEHFGKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGJ4</accession>
    <name>A0A1A0HGJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713454.1">
        <property type="nucleotide sequence ID" value="XM_018856943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGJ4"/>
    <dbReference type="GeneID" id="30029919"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291791" evidence="1">
        <location>
            <begin position="23"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="64" mass="7262" checksum="5555E87E5ED0D1B9" modified="2016-10-05" version="1" fragment="single">KKIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPIYYIEDTLRSLKVTLFCFCKSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS2</accession>
    <name>A0A1A0HGS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_104685</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713855.1">
        <property type="nucleotide sequence ID" value="XM_018853775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS2"/>
    <dbReference type="GeneID" id="30026751"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03749">
        <property type="entry name" value="proteasome_alpha_type_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000016">
        <property type="entry name" value="Proteasome subunit alpha type-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035144">
        <property type="entry name" value="Proteasome_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="8">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="239"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="296" mass="32509" checksum="D32F70ED22149BB5" modified="2016-10-05" version="1">MFRNNYDNDSVTYSPTGRLFQVEYALEAIKQGSAAVGIASANHVVLVALKRNAEDLGSYQKKIIKVDDHMGIALAGLAPDARVLSNYLRKLAMNSKLVFNRPLLTSKAAFSCADKAQENTQLYGSRPYGVGLLIGGWDETGAHLYEFQPSGSVLEYYGAAIGARSQAARTYLERNLEVIRGCQDIESLVVHGLNALRDTLSQDMELTIRNTTVSVVGKDQEFVIYEDEDVQRWLGKLDSTSNARNNDDDDDDEEGDNAEKAHSAPKDKSKDQADTEEKPQVTGGDASETSDKMETD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGY7</accession>
    <name>A0A1A0HGY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_99069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713594.1">
        <property type="nucleotide sequence ID" value="XM_018859485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGY7"/>
    <dbReference type="GeneID" id="30032460"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="91" mass="9770" checksum="6CF5B67CE3CF4903" modified="2016-10-05" version="1">MPRACKPSTYQEFCPRTIVFGAPSNCFVLFLVVLFLVGSREESVVICITAPLGITAVPPARASSLVGTRLGACFRIACIPAFISWAKLCFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH39</accession>
    <name>A0A1A0HH39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37138</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713975.1">
        <property type="nucleotide sequence ID" value="XM_018856827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH39"/>
    <dbReference type="STRING" id="869754.A0A1A0HH39"/>
    <dbReference type="GeneID" id="30029803"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF15">
        <property type="entry name" value="SPORULATION PROTEIN RMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="4">
        <location>
            <begin position="184"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="407" mass="46531" checksum="676B4E9803FC237A" modified="2016-10-05" version="1">MLSEQSPLLEQPPAGTAPPAWQANKTGSRLSQLPKIGLQRTSRTSQKLKLLPVVPDLGAEDDDGLDEAPFAAAGGQRHGVYSQVTKIKDRPARKDAEILGKLHRDLLPRVTAYCTCGLYRMKELLRWLKDRKRIHNTGPRLFDECLYSPFTYKDWRSDSDGGSRKLTRLADDGGEIEFGTRSDVFVFEYGVVIMWGFSVKEEHAFLEDLARFESEKLLAEDIQVEEFNYYITTSYQPRIYNDFITLRDDNNHMLKLLVSHALAQLVKISLFEELVDNTIEDTHDIPQQIAHTGKVEMTRDETMKLIGELFILRININLHGSVLDSPELMWAEPHLEPIYQATRGYLEINQRVELLNQRLEVISDLLQMLKEQLGHSHEENLEFVVVLLVCIEVVVSVVNIVVDILTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH63</accession>
    <name>A0A1A0HH63_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714000.1">
        <property type="nucleotide sequence ID" value="XM_018859459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH63"/>
    <dbReference type="STRING" id="869754.A0A1A0HH63"/>
    <dbReference type="GeneID" id="30032434"/>
    <dbReference type="OrthoDB" id="5103085at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.20">
        <property type="entry name" value="Centromere DNA-binding protein complex CBF3 subunit, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038279">
        <property type="entry name" value="Ndc10_dom2_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="381" mass="43479" checksum="D1F11CF52ABC2D51" modified="2016-10-05" version="1">MSAILNFEDNSVYYSVEDNMDGEEENYGQSDEFLSPRDTETVQPDSETPQSIYVEAMSQSERSFSNQTNFVVDGKFMLECVKNILFSATEVPASQESKIGPKFIPLFKYVLQVLQKCLGIYKTAKIHDKQERAEQIKRKWLNILYLGLLDDYHREKLKTVFKQSKKKHLNKNNAKMFQKKYSIADCKNMMINMLEKTGSGRKKDIYDGINATLEFLLGHHLMLRGLLKTELELSGSVYSEEETSHGTVPILGFQLVRENTRGRSSLPGILGVCRNKDVEICLVSAFALSLWYRFDFQNQHAPLSGGRALNFMDKGSWYDPKVLFASSDESKHHRPISPAYAENLVSMCLDSIGFESLKKSRLGRKKNGEKVEVCLVEEARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHK3</accession>
    <name>A0A1A0HHK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SPRY-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_85705</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713966.1">
        <property type="nucleotide sequence ID" value="XM_018859252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHK3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHK3"/>
    <dbReference type="GeneID" id="30032227"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12910">
        <property type="entry name" value="SPRY_SSH4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035780">
        <property type="entry name" value="SPRY_Ssh4-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508835" evidence="5">
        <location>
            <begin position="21"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="6">
        <location>
            <begin position="80"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="373" mass="42168" checksum="DE1CF99B56D3DB57" modified="2016-10-05" version="1">MLVAVYVFVAVASGLPLVALLDTFRACDVDHAFLNDEESLMALAEEHDFPTLSPEEQRAYLEGEEFLKLHPPDPAHARGKAFTRDDELVIKDCGINAFEFEQEHDILAPRFVVEDRTEINFTNNARLYSTATAVLRFPLPIKNRLPDTVYFEVKVFEYAEDVPNAHFAIGLVTKPYPSQFRLPGYNNFSMAYESTGNLKINKPFPTPLQQHRGTESQYNALVLPPLKQLDVVGFGYVVSSGTYFITRNGKKLMDVMKGCHLDLYPAVGCFSTNGRFQVNFGQMGFVWIEANVRKYGFFSASDRRFAGQRGLASLPEYGNSILLNDKLLDKGDELPPEYPEEELDFFGRKIRVGTSAQFHGKKLNEDSEFEVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHR8</accession>
    <name>A0A1A0HHR8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_110848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714185.1">
        <property type="nucleotide sequence ID" value="XM_018853916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHR8"/>
    <dbReference type="GeneID" id="30026892"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="139" mass="16300" checksum="EB9A1417AA21A38E" modified="2016-10-05" version="1">MFSSLSLISSLSLISSLSYGLFSFLWSLSFILFSLCCSLYLILFFSFILFSFLCSLLFLMFSSLSRDVFYFLCSLLFLMFYSLSCVLCSFYDLFSLFRSLLFLMFSSLYFVLFLFFSLFLLLSSLSFDVFSFFDPFSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHS0</accession>
    <name>A0A1A0HHS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">asparagine synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="2">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_30001</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714036.1">
        <property type="nucleotide sequence ID" value="XM_018855530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHS0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHS0"/>
    <dbReference type="GeneID" id="30028506"/>
    <dbReference type="OrthoDB" id="409189at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070981">
        <property type="term" value="P:L-asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000031">
        <property type="entry name" value="Asparagine synthase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000050">
        <property type="entry name" value="Asparagine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006949">
        <property type="entry name" value="PRK09431.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="14">
        <location>
            <begin position="2"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="11">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="366"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="13">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="574" mass="63958" checksum="7669C0BA85E3760A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHX5</accession>
    <name>A0A1A0HHX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-22 sterol desaturase ERG5</fullName>
            <ecNumber evidence="6">1.14.19.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytochrome P450 61</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ergosterol biosynthetic protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_76496</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.41" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713963.1">
        <property type="nucleotide sequence ID" value="XM_018858540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHX5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHX5"/>
    <dbReference type="GeneID" id="30031516"/>
    <dbReference type="OrthoDB" id="1372046at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000249">
        <property type="term" value="F:C-22 sterol desaturase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000021">
        <property type="entry name" value="Cytochrome P450 61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286:SF228">
        <property type="entry name" value="C-22 STEROL DESATURASE ERG5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24286">
        <property type="entry name" value="CYTOCHROME P450 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="5 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="524" mass="59479" checksum="F23FA75E10FD990D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHY9</accession>
    <name>A0A1A0HHY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Altered inheritance of mitochondria protein 24, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_106434</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AIM24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713978.1">
        <property type="nucleotide sequence ID" value="XM_018853817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHY9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHY9"/>
    <dbReference type="GeneID" id="30026793"/>
    <dbReference type="OrthoDB" id="5295771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002838">
        <property type="entry name" value="AIM24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36959:SF2">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 24, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01987">
        <property type="entry name" value="AIM24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="409" mass="45802" checksum="E55E95EBBFF4864B" modified="2016-10-05" version="1">MTSYAVLPARAAQPRFFWARGLSFNQAPKALGPVPVVFPGVNLLPALDAVEELPTLRSVDDVRLEALGSPPQALSVAAPPSLPVYIRRNTMMTLQANMDHVRFQSRLLAPLQRLMSGNFVLRYQEIVGTEPFSVLVLASSPSWFPSLFSRTTQKSFTNLALDGAADWALFKRDALQVYAGPSLEISMRRVPARISRRFSKHLKLRLREPTGLYRWYRPGYTFVHGRGALGLVGNGMVYSASVAEGELIAVSRAHLLGISVNGPHDLENCIVAHKNPVKMENVVLVPPPQAASLNTWRDVWTQLKYYYWRLFDLLRKFRGRSMQQAIGNQDFVKVVGPRTILLQSGVPQESFERKFDLPRLTPASHVQIVPEKLVENKPADYLNVVTIEPGKAPKIESTDDFKDGLRHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI70</accession>
    <name>A0A1A0HI70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_114473</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714345.1">
        <property type="nucleotide sequence ID" value="XM_018853990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI70"/>
    <dbReference type="STRING" id="869754.A0A1A0HI70"/>
    <dbReference type="GeneID" id="30026966"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12409:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016396">
        <property type="entry name" value="Prefoldin_subunit_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="142"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR016396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="196" mass="22923" checksum="FBFB3E4A081272B6" modified="2016-10-05" version="1">MAEFLKPKKTNPRGIPEAPFIEKVESIIKNPEKEFEATLQAFQDRLQQYKYMELSKQQQLADLKIKIPDIEKNLAIINHIKERKESGDDDQLLETNYELDSTLYSRAEVDLQALDSVGLWLGADVMLEYPLDEAVELLNTRLQNNVQLLETVEEDLDFLRQNITTMEVNTARLYNWDVERRKALRNVEQNTQNLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI96</accession>
    <name>A0A1A0HI96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoenolpyruvate carboxykinase (ATP)</fullName>
            <ecNumber evidence="3">4.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_108815</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>oxaloacetate + ATP = phosphoenolpyruvate + ADP + CO2</text>
            <dbReference type="Rhea" id="RHEA:18617"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.49" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714088.1">
        <property type="nucleotide sequence ID" value="XM_018853871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI96"/>
    <dbReference type="STRING" id="869754.A0A1A0HI96"/>
    <dbReference type="GeneID" id="30026847"/>
    <dbReference type="OrthoDB" id="184182at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004612">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00484">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000001">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000002">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [ATP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00453">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001272">
        <property type="entry name" value="PEP_carboxykinase_ATP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015994">
        <property type="entry name" value="PEPCK_ATP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00224">
        <property type="entry name" value="pckA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006820">
        <property type="entry name" value="PRK09344.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006821">
        <property type="entry name" value="PRK09344.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031:SF0">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30031">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01293">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006294">
        <property type="entry name" value="PEP_crbxkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00532">
        <property type="entry name" value="PEPCK_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="553" mass="61003" checksum="2FDAE22EC67D947B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIF7</accession>
    <name>A0A1A0HIF7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED19</name>
        <name evidence="11" type="ORF">METBIDRAFT_9914</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714148.1">
        <property type="nucleotide sequence ID" value="XM_018859487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIF7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIF7"/>
    <dbReference type="GeneID" id="30032462"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08633">
        <property type="entry name" value="Rox3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="124"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="168" mass="18672" checksum="5293BD06B23EA209" modified="2016-10-05" version="1">MENVDQIKRPLEGVYLIDQEATYNAPNPTPLDNLLAMYDLELIASSLGRTKPDGSKGVKLRKSYKNHIQDLKGKHQIPPSKPLNGQILDPELAKIPDVITEFNPELLSAALKFDKTSINGIPGFSPSDLALSDQAASTRGDDNGESEEAKRKRKKKQLMGVEYKRHHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIG4</accession>
    <name>A0A1A0HIG4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_110480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714153.1">
        <property type="nucleotide sequence ID" value="XM_018853908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIG4"/>
    <dbReference type="GeneID" id="30026884"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="81" mass="9398" checksum="73BF9E73D9B1B650" modified="2016-10-05" version="1">MECCMYKTYSRRERKVIQDYTWWAIFLACISQIFEAATGQIGKKPIMCDNLQYTIEKSPHTSRALGKYMAMLQPDPASIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HII4</accession>
    <name>A0A1A0HII4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Small oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_85958</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714292.1">
        <property type="nucleotide sequence ID" value="XM_018859254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HII4"/>
    <dbReference type="GeneID" id="30032229"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901584">
        <property type="term" value="F:tetrapeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="565"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="633"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="710" mass="79736" checksum="EBC779F596DECD62" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HII8</accession>
    <name>A0A1A0HII8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cell wall synthesis KRE9KNH1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30211</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell wall beta(1-&gt;6) glucan synthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRE9/KNH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714297.1">
        <property type="nucleotide sequence ID" value="XM_018855573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HII8"/>
    <dbReference type="STRING" id="869754.A0A1A0HII8"/>
    <dbReference type="GeneID" id="30028549"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042546">
        <property type="term" value="P:cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045328">
        <property type="entry name" value="Kre9/Knh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008659">
        <property type="entry name" value="Kre9/Knh1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28154:SF1">
        <property type="entry name" value="CELL WALL SYNTHESIS PROTEIN KNH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05390">
        <property type="entry name" value="Kre9_KNH1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291851" evidence="4">
        <location>
            <begin position="20"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="6">
        <location>
            <begin position="26"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1 C-terminal" evidence="5">
        <location>
            <begin position="166"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="269" mass="28811" checksum="52948396B93B2EF1" modified="2016-10-05" version="1">MHFLSLWIILVSLAARVLGDVSITSPSTDQTYSGSSGSASVSVVWTDDSDDDADASLSNVDLYAIVLCTGSNSDIQSVMTLTDSLSSSALEYDAVIDEADVPSGVYFIQVYAKFGSGYTIHYSNRFTLTGMSGSTSTYTFSASLFSITGTHPGAQLNLDGETTLIDSRSFSVTYTLQTGKTRYAPMQTQPGSSISHSVYSTRHATSAYTPYSTIRASPNVYSTVTPGWSYAVTSTFNTAAVAEYPTYYYPASSRVVSASLSSANKKRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIL6</accession>
    <name>A0A1A0HIL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisomal targeting signal receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Peroxin-5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_30112</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Receptor that mediates peroxisomal import of proteins containing a C-terminal PTS1-type tripeptide peroxisomal targeting signal (SKL-type). Binds to cargo proteins containing a PTS1 peroxisomal targeting signal in the cytosol, and translocates them into the peroxisome matrix by passing through the PEX13-PEX14 docking complex along with cargo proteins. PEX5 receptor is then retrotranslocated into the cytosol, leading to release of bound cargo in the peroxisome matrix, and reset for a subsequent peroxisome import cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via WxxxF/Y and LVxEF motifs) with PEX14; promoting translocation through the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxisomal targeting signal receptor family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714164.1">
        <property type="nucleotide sequence ID" value="XM_018855559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIL6"/>
    <dbReference type="GeneID" id="30028535"/>
    <dbReference type="OrthoDB" id="10006023at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005052">
        <property type="term" value="F:peroxisome matrix targeting signal-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140597">
        <property type="term" value="F:protein carrier chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000218">
        <property type="entry name" value="Peroxisomal targeting signal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024111">
        <property type="entry name" value="PEX5/PEX5L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130:SF0">
        <property type="entry name" value="GH08708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10130">
        <property type="entry name" value="PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR PEX5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="9 18" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="13" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="331"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="441"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="99"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA23683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="591" mass="67025" checksum="1441D353D55E32FC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIM6</accession>
    <name>A0A1A0HIM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit RPB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_38386</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714490.1">
        <property type="nucleotide sequence ID" value="XM_018856997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIM6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIM6"/>
    <dbReference type="GeneID" id="30029973"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001988">
        <property type="entry name" value="PRK00783.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="8">
        <location>
            <begin position="21"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="33945" checksum="1E6C812318B59030" modified="2016-10-05" version="1">MEIDASEAGPRVTIRQTERDRVDFILRGVDLSLANSLRRTMLAEVPTIAIDLVEIDVNTSVLADELLSHRLGLVPLCSEGIENLLYSRDCTCDQYCPNCSVKLELTAKCDSDSTMNVYASDLAKFHNGSRLGDPVARDAQQRGPLLCKLRKHQELRLTCIAKKGIAKEHAKWSPCSAIGFEYDPWNKLKHTDYWHEVDAEAEWPRSANCEWEEAPDPEAPFDYSAKPGNFYMDVETVGNLPPNEVVVRGIETLQLKLAGIAVELNKDSVEANSTAAGGFTTYGRSPGGADGDSPANGYAYGEGFGNSAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIT2</accession>
    <name>A0A1A0HIT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF336-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_38193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714401.1">
        <property type="nucleotide sequence ID" value="XM_018856968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIT2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIT2"/>
    <dbReference type="GeneID" id="30029944"/>
    <dbReference type="OrthoDB" id="2209940at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="entry name" value="Haem-degrading domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010371">
        <property type="entry name" value="YBR137W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28255">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28255:SF1">
        <property type="entry name" value="UPF0303 PROTEIN YBR137W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="HbpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008757">
        <property type="entry name" value="UCP008757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="GlcG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18530" checksum="4E2C748294D76166" modified="2016-10-05" version="1">MSNAFFSYSLEEIDHYESDKIALPHFDSSIAWQIGLYAHKLAQGYDKPMLIDISLSNGQVLFHAPSKNGIVLDNEHWVTRKKNTAFRFGKSSFYMGRKMAMKQMSAEKALFVDSKEYAFHGGSVPIKISGTDQICGALTVSGLAQIDDHLFVLQVLKDTRHSLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ26</accession>
    <name>A0A1A0HJ26_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_35672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714640.1">
        <property type="nucleotide sequence ID" value="XM_018856640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ26"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ26"/>
    <dbReference type="GeneID" id="30029616"/>
    <dbReference type="OrthoDB" id="5354116at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="553" mass="61205" checksum="E2181CC684D70535" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJC8</accession>
    <name>A0A1A0HJC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M16 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_17281</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714601.1">
        <property type="nucleotide sequence ID" value="XM_018854700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJC8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJC8"/>
    <dbReference type="GeneID" id="30027676"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="7">
        <location>
            <begin position="37"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="8">
        <location>
            <begin position="202"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="9">
        <location>
            <begin position="809"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="982"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="982" mass="108526" checksum="2E9C3ACC71BB2573" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJQ0</accession>
    <name>A0A1A0HJQ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_120813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714707.1">
        <property type="nucleotide sequence ID" value="XM_018854161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJQ0"/>
    <dbReference type="GeneID" id="30027137"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="20541" checksum="37D2771E47E03651" modified="2016-10-05" version="1">MSRESWCDWREWARFARVVVRFMQRPRPARRANILIAPVADTPQFSARNAAPAPGHASWPRPHVRRNQCARSSQYLPQGVVTSFVVGCGPAVCSAPGNDCTPARAAEFYWMATSRLQRHGHWVEAENSEQSKKKQKKNKKKKKRKVPPGICLVSGPWLCVLRRGQMPSPWICHSSSGQKRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJR2</accession>
    <name>A0A1A0HJR2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Brix-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_34430</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714534.1">
        <property type="nucleotide sequence ID" value="XM_018856472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJR2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJR2"/>
    <dbReference type="GeneID" id="30029448"/>
    <dbReference type="OrthoDB" id="10261452at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045112">
        <property type="entry name" value="PPAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661">
        <property type="entry name" value="PETER PAN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12661:SF5">
        <property type="entry name" value="SUPPRESSOR OF SWI4 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="2">
        <location>
            <begin position="25"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="412" mass="46412" checksum="E19C54C172F8381B" modified="2016-10-05" version="1">MAKRRVKKRTHQGPNPEEVAKIPKSMVLALGASLKNRSLAQLVKDFRLVMEPHTAIKLRERKANKLKDFVVMSGPLSVSDLFVFKQSQTSGNVSLRVAKMPKGPSLQFKVNSYSLMKDVRRILKHPKSAGRASAAFKQPPLLVMNGFSRKVREMENHEKLMITIFQNLFPPIQPQKTRVGSIQRVLLINKMENGEIEMRHYAISTKLVEESRNIKKLINSHHNKTKSLPNLTKHTDISDLVLDPYAVGGLTSDSEVEDDAVVEIQNTQEHSLKKKAVEEPPASATRKRAIKLQELGPRINMSLVKIEEAILGSSKTIYHANIHKLKEEEKALEKKHQIKFKEKAERRAVQKANVDAKQAKKDAKKARKLARKNGESATQDSGDEDNSGDSDNEPQINPSDYDNDSDLFSDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJS1</accession>
    <name>A0A1A0HJS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_121620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714746.1">
        <property type="nucleotide sequence ID" value="XM_018854182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJS1"/>
    <dbReference type="GeneID" id="30027158"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="57" mass="6785" checksum="E032E24B4FD93A53" modified="2016-10-05" version="1">MLNLTYRPSSVFWTSSLHIYNRCLCPRLQRKTGLPLWLLIFLERAFLTTLAWIDVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK28</accession>
    <name>A0A1A0HK28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_10501</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714837.1">
        <property type="nucleotide sequence ID" value="XM_018853783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK28"/>
    <dbReference type="STRING" id="869754.A0A1A0HK28"/>
    <dbReference type="GeneID" id="30026759"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="5">
        <location>
            <begin position="28"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="320" mass="36686" checksum="D216C738AC6A0AF0" modified="2016-10-05" version="1">MIRTVKPKNARSKRALAKKEAKLVENTKQALFVPGGSGNKFLHDVMVDLMALKKPEAKKFSKKNDIRPFEDASGLEFFAEKNDCSLMVLSTHNKKRPNTLIFSRFFNHKVYDMIELSIQSDPKLFSDFRKVTFPVGLKPMFTFNGPAFDSHPVYQQIKSYFLDFYRGDETDLQDVAGLQYIIALSVGEIEDPNSLTLPLLHFRVYKLKTYKSGQKIPRVEVDEIGPRFDFKIGRRTTPAPDVEKSALSRPKQLQAKVKKNVTTDFMGDKVAKIHVGDQDLSKMQTRKMKGLKAKYDQVDEAEFDEEDYGVEESENKRQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK60</accession>
    <name>A0A1A0HK60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone H2A.Z-specific chaperone CHZ1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Histone H2A.Z-specific chaperone chz1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_10348</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms a chaperone-bound H2A.Z-H2B complex that acts as a source for SWR1 complex-dependent H2A to H2A.Z histone replacement in chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CHZ1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714669.1">
        <property type="nucleotide sequence ID" value="XM_018853747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK60"/>
    <dbReference type="STRING" id="869754.A0A1A0HK60"/>
    <dbReference type="GeneID" id="30026723"/>
    <dbReference type="OrthoDB" id="4026609at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="9">
        <location>
            <begin position="169"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="225" mass="25159" checksum="39570E547B6C199A" modified="2016-10-05" version="1">MAASKDDIQNGSIEATPELEEIREPDSVYEPGKDDAAEPANETVDAGEVGKELGELKDDSLDENAEKQAAQTEEADRDKPEAEEAGKPEVEEAEKSEEGKENTESKDEGQELKTLGNTKKRTEGNGTQDPQKRKRQRRRQYDDAPKEEPESEEGEEEKIKHDENDDDDDDDDDNLGGVDESNIISGGRRTRGKKIDYTKANEELQLPEDDEEDDEEFEDKGGDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKB8</accession>
    <name>A0A1A0HKB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Outer spore wall protein RRT8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10483</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714813.1">
        <property type="nucleotide sequence ID" value="XM_018853777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKB8"/>
    <dbReference type="STRING" id="869754.A0A1A0HKB8"/>
    <dbReference type="GeneID" id="30026753"/>
    <dbReference type="OrthoDB" id="10012223at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005619">
        <property type="term" value="C:ascospore wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052786">
        <property type="entry name" value="Spore_wall_assembly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34292:SF2">
        <property type="entry name" value="OUTER SPORE WALL PROTEIN LDS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="33218" checksum="26A3225FBC3D7FFD" modified="2016-10-05" version="1">MIEEKKCNWKVALSDACFNNSFMAPFKGIQYFANDSQLWMPFLAICVPSALILILVFSTLLITVFPPYLAMVVMVTGPFGFISAFITLLDVTRRITVTLLQFLFMRRIQASIFERVLTNEGAAEIVARYTDMRGRTIHDSSLEFRLCNQTLPFLLREILYTILGIIPLIGPLVVLIAKAPIKGYKAHKHYFKLEMWDHAQINHFFKKYHSDYTSFGFIALILEMIPAFTICFMYTNNIGIALWTAKYHRDFEEFISREATRPEGETEGLEFWGNSVENSKKGQGGAEKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKD7</accession>
    <name>A0A1A0HKD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CASTOR ACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714966.1">
        <property type="nucleotide sequence ID" value="XM_018856911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKD7"/>
    <dbReference type="STRING" id="869754.A0A1A0HKD7"/>
    <dbReference type="GeneID" id="30029887"/>
    <dbReference type="OrthoDB" id="58529at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046394">
        <property type="term" value="P:carboxylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051719">
        <property type="entry name" value="CASTOR_mTORC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31131:SF6">
        <property type="entry name" value="CASTOR ACT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31131">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASTOR ACT" evidence="1">
        <location>
            <begin position="151"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="423" mass="47118" checksum="D6EC515F467A35D9" modified="2016-10-05" version="1">MSGQVYLNPSRLSILSIPRDKVWLFSSAILKLLHQEAKQAPQSNFKSDSKNDHSTSEDLLDLDAGFTDDSTLYDSEEISKMTSSTLSELSLKADLSVSLPLISDEDHHFFHIAYTPSECTVILSTYLSSELFMKPLSVCKKLGYSDVILLEKSYLNLQVDSEGEFNNSAKILELTKPFSQHNISLFFLSTHFTNIVLIPYDLKDQVIEILTENDFVFSDVSNSYILSQNYSSLSKTYPKDTVVCPATSEVNRRDTMNILADTKITPRIHRKVKLLLTGARPGQVKQSIVKASQTIAADAVPDYFAITRSSFNEISLILPGSSKQRALMGFDFRSIIGSALDVIIPVTVDLSKLPIDSAGIVAGLASSLWESVRSLNEPATERFDMNYLSMARSGVIMIPRENLDVVTRMVKNMRTDLKNNVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKM3</accession>
    <name>A0A1A0HKM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pex24p integral peroxisomal membrane peroxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_10677</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715022.1">
        <property type="nucleotide sequence ID" value="XM_018853823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKM3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKM3"/>
    <dbReference type="GeneID" id="30026799"/>
    <dbReference type="OrthoDB" id="5586090at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006614">
        <property type="entry name" value="Peroxin/Ferlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052646">
        <property type="entry name" value="Peroxisomal_PEX28-32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010482">
        <property type="entry name" value="TECPR1-like_DysF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31679:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX30-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06398">
        <property type="entry name" value="Pex24p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00693">
        <property type="entry name" value="DysFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxin/Ferlin" evidence="6">
        <location>
            <begin position="277"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="408" mass="47622" checksum="6FED8F1E3D22144E" modified="2016-10-05" version="1">MSDNIVYALFEPTEPKLLSTLTQAQLLVDNPLLASALSNIFPYLLVIDNALKIVTWTNDDPFLNILFVALYSVIVLYWKYLKYWFIPSLAALVFSSVVWNTSSIMHDAKFDEKPTIDEVLLTLHNITVRFELLLRPAKHLDMSMRNYIKMVLGAVILSPIHLVIVKFLFLPQTCLWFVGVLALTYHSPFSFAVRRLLWRSAYLRKVIYHLTGLNIRLMRSNYSSPTSRAHETISRTHSPSGTDVEVQASSNPIADTVQAAINFVILKKVVVSLTQLKHVVRYDVLENERRWLGLGWSKYLLPNERASFCYEKLMLSAPDPHTDAEFVFPVYENDLFTYQWQWVDDKWSIDLEYNRSRFPTGWVYYDKNWGDQKYEDGFSRYTRTRKWTRRAILLIDKRAEVYDEQSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKM8</accession>
    <name>A0A1A0HKM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Actin-like ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_34768</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715027.1">
        <property type="nucleotide sequence ID" value="XM_018856523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKM8"/>
    <dbReference type="STRING" id="869754.A0A1A0HKM8"/>
    <dbReference type="GeneID" id="30029499"/>
    <dbReference type="OrthoDB" id="337660at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="38919" checksum="A992432AEAC11F61" modified="2016-10-05" version="1">MPDEYFAVILEFNSCEIKLGFAGEASEHVRLTLRSPIWKNLVPPITSSLSLPSFLQIDSHAIPNNLRTEILRELSESKTYSNMLQAYNKDQRDLKWFAWGLDNFHTLATIVKVLLHTRLLVSPLLTKLFVIDRSMSALEKSTFCKKFLMMRTTASVTFLPYSPCCCVGAGVENALVVSLEWESCKVVPVVDLRSLPAKEYIQFSGEATHYSPLIPHDAKSFEDIEIEIQTNEKILEAAFFSENSLPKAIAALIQTLELDCRACVVANIIFTGLRSDIPGMKGRFLSEASTFFPKCNVAGKISLGAWSGASLYCSTTLLNEESSKWKHMEISRVNFEKTASAALEAYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKP3</accession>
    <name>A0A1A0HKP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">TATA-binding protein-associated factor mot1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Modifier of transcription 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_135132</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715042.1">
        <property type="nucleotide sequence ID" value="XM_018854381.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HKP3"/>
    <dbReference type="GeneID" id="30027357"/>
    <dbReference type="OrthoDB" id="10252227at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045898">
        <property type="term" value="P:regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17999">
        <property type="entry name" value="DEXHc_Mot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000009">
        <property type="entry name" value="B-TFIID TATA-box-binding protein-associated factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000428">
        <property type="entry name" value="TATA-binding protein-associated factor 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044972">
        <property type="entry name" value="Mot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044078">
        <property type="entry name" value="Mot1_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022707">
        <property type="entry name" value="Mot1_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498:SF1">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12054">
        <property type="entry name" value="DUF3535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="1312"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="1672"/>
            <end position="1827"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="183"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="694"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA24561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1901" mass="212129" checksum="66419F091B95DBB5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HL68</accession>
    <name>A0A1A0HL68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mediator of RNA polymerase II transcription subunit 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Mediator complex subunit 8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MED8</name>
        <name evidence="11" type="ORF">METBIDRAFT_135267</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715039.1">
        <property type="nucleotide sequence ID" value="XM_018854383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HL68"/>
    <dbReference type="GeneID" id="30027359"/>
    <dbReference type="OrthoDB" id="5329317at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019364">
        <property type="entry name" value="Mediatior_Med8_fun/met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13074:SF9">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10232">
        <property type="entry name" value="Med8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0010">Activator</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="214" mass="24284" checksum="8062C181820176C9" modified="2016-10-05" version="1">MSKLQPETPVVNLNPQNDIPIESLELIRNRLHQVYQSLRKLADQINYTNRNPKARLPSYLNLHGQFQVLITQLHSIASQLDHNSEVLGSTNAYPLPNFPTTQYEGLVTTLLRKKPLPAVDEWVDEAIKSCETFKISVDKDNEIAEWCQLKVKELADEYNFDGFLTEDERSAMETDQDANTGDIDKGGRAAQFVTSKPPMDPNVVMKFMTHGVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYX6</accession>
    <name>A0A1A0GYX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_14033</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. LDAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709255.1">
        <property type="nucleotide sequence ID" value="XM_018854473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYX6"/>
    <dbReference type="GeneID" id="30027449"/>
    <dbReference type="OrthoDB" id="448051at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019363">
        <property type="entry name" value="LDAH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13390:SF0">
        <property type="entry name" value="LIPID DROPLET-ASSOCIATED HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10230">
        <property type="entry name" value="LIDHydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA16956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="336" mass="38307" checksum="60A8D14EB18AFA67" modified="2016-10-05" version="1">MTIRTNLEQPLTSEYHVTLAQGAADSPLLVFIPGNPGLIDYYTTYLDLISVEYPDHDILAISHAGFQTSGDFVADAAAQKYVFYDLEYQIRHKCSIIKKYIAQGHTRLSFLCHSVGGYITQRVVARLLADKNVGPKISIEFIGLVCPTIVDIAKSDSGVFFTKLFTYLPLIQLAVWFLVVLQTIFLDSLARKIISKLVIARPQATCDKLKEAWQNSIRATFKIYKSKRITRQTLELAREELQVIHRDDDINDWFFNELPASHGTRLWCFFAASDYWVHDNLRDYILGRYHDQDNLHVRFQIGEPSTKSTPAITHSFCIDQSVEFAEITCAALRFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYY6</accession>
    <name>A0A1A0GYY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_48106</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709265.1">
        <property type="nucleotide sequence ID" value="XM_018858261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYY6"/>
    <dbReference type="STRING" id="869754.A0A1A0GYY6"/>
    <dbReference type="GeneID" id="30031237"/>
    <dbReference type="OrthoDB" id="30826at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF4">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="7">
        <location>
            <begin position="165"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="437"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA16966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="472" mass="51895" checksum="7E8F5364B024B1EE" modified="2016-10-05" version="1">MFPESTVVPVAEAATLCWQHPAIRKTRPVALYMGDMRLGSDFARVLDDTSYSARTDDSCVAFRVEVHAAARADTHLLDMHEYLVDSDLSIAKVDQASAHFAWEQGPAPDAKTTGAPPATDTPPATGAPRKVPVALRSLTPAFKFSNFDLVALDPGLQRAAALQLFSALDVFGFIPERRVSPELLTGEALYVTPDKASSVKNTLGFQAFCDALRTRKLAALCRFVRKQEKEVEVGVAYAVRVKSSLGHTSCFVFVRLPYREDEKIGRFPLLSGPADAAEPQPPAAALLMDAFVNEKTYESDHELSEELASHDVALIDNFKVTMKTGDSSKLPRPPVRKGYDPFICSSPSAARHQAHMRQILLRSLGAADWVRHFDNPRFVADNLRGLALSTNFFNLRNCLAVNSQALRPDWLLDLARKSHDSSKRLASELGCTYVRKADSKKAKTQASAAHLRQKGNYGADEGDYDEIPDFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ14</accession>
    <name>A0A1A0GZ14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Probable electron transfer flavoprotein subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_48144</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </cofactor>
        <text evidence="7">Binds 1 AMP per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000012">
        <property type="protein sequence ID" value="OBA16942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709244.1">
        <property type="nucleotide sequence ID" value="XM_018858266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ14"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ14"/>
    <dbReference type="GeneID" id="30031242"/>
    <dbReference type="OrthoDB" id="276685at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000011">
        <property type="entry name" value="Electron transfer flavoprotein subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294:SF8">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="8">
        <location>
            <begin position="27"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00070315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA16942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="255" mass="27668" checksum="D4BE727B48022B71" modified="2016-10-05" version="1">MSTKLKILVPVKRVIDYAIKPRINKAGTGVETKGVKFSMNPFCDIALEESLKIREANKGLVEKIHAVSIGPTKSQDILRTALAKGADSTTLVDVGETEVEPLTVAKMLQKVVEREQSNLVILGKQAIDDDLNQTGQILAGLLNWPQATNASKVEIDGDKISVTREVDGGSDTLQASLPMIVTADLRLNEPRYASLPNVMKAKKKPLEKLKASDLGIEIVNRVETLKVEEPPTRSAGVKVESVDELIAKLKEAKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ83</accession>
    <name>A0A1A0GZ83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein acetyllysine N-acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.286</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_27572</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709304.1">
        <property type="nucleotide sequence ID" value="XM_018855338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ83"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ83"/>
    <dbReference type="GeneID" id="30028314"/>
    <dbReference type="OrthoDB" id="420264at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099115">
        <property type="term" value="C:chromosome, subtelomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046970">
        <property type="term" value="F:histone H4K16 deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045950">
        <property type="term" value="P:negative regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017328">
        <property type="entry name" value="Sirtuin_class_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF6">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACETYLASE SIRTUIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037938">
        <property type="entry name" value="SIR2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="11">
        <location>
            <begin position="2"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7 10">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="28"/>
            <end position="32"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="38"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9 10">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="207"/>
            <end position="208"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="231"/>
            <end position="233"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037938-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA17007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="32408" checksum="0788F21BE0196463" modified="2016-10-05" version="1" fragment="single">MPLPLEKQLVPLIKALQEKKKVTFFLGAGISTSCGIPDFRSPKTGLYANLKRLNLPYPEAVFDIDYFRENPKAFYTLCDELYPGKFFPSKFHFLLKLVHDNDLLARIYTQNIDTMESLVGIHPDKIVAAHGSFATNHCIECAEPMETEDLVKLMGDKSVNDGIPQCGKCKGYVKPDIVFFGEALPVRFFDMWEEDCDNVDIAIVAGTSLTVYPFAGLPAECHKTSLRVLVNNEVVGDFKTKKRKTDLLITNDCDVVAETIAKELGWEQQLQKLISEEKKKFDAKSKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H204</accession>
    <name>A0A1A0H204_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sphinganine-1-phosphate aldolase</fullName>
            <ecNumber evidence="14">4.1.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Sphingosine-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_226501</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 16 17">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.27" evidence="14"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709383.1">
        <property type="nucleotide sequence ID" value="XM_018855180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H204"/>
    <dbReference type="STRING" id="869754.A0A1A0H204"/>
    <dbReference type="GeneID" id="30028156"/>
    <dbReference type="OrthoDB" id="10254570at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008117">
        <property type="term" value="F:sphinganine-1-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000020">
        <property type="entry name" value="sphingosine-1-phosphate lyase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 17 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16 17" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="16">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA17988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="590" mass="65026" checksum="A8A48BF89A89542B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H272</accession>
    <name>A0A1A0H272_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carbon catabolite-derepressing protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_47785</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Essential for release from glucose repression. It interacts and has functional relationship to the regulatory protein SNF4. Could phosphorylate CAT8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709448.1">
        <property type="nucleotide sequence ID" value="XM_018858221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H272"/>
    <dbReference type="STRING" id="869754.A0A1A0H272"/>
    <dbReference type="GeneID" id="30031197"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000144">
        <property type="term" value="C:cellular bud neck septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005641">
        <property type="term" value="C:nuclear envelope lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004679">
        <property type="term" value="F:AMP-activated protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061762">
        <property type="term" value="P:CAMKK-AMPK signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071940">
        <property type="term" value="P:fungal-type cell wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010920">
        <property type="term" value="P:negative regulation of inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900436">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045722">
        <property type="term" value="P:positive regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000222">
        <property type="term" value="P:positive regulation of pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000217">
        <property type="term" value="P:regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090606">
        <property type="term" value="P:single-species surface biofilm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12122">
        <property type="entry name" value="AMPKA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14079">
        <property type="entry name" value="STKc_AMPK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14334">
        <property type="entry name" value="UBA_SNF1_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000544">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000236">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.80">
        <property type="entry name" value="Kinase associated domain 1, KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032270">
        <property type="entry name" value="AMPK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028375">
        <property type="entry name" value="KA1/Ssp2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013896">
        <property type="entry name" value="SNF1_UBA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16579">
        <property type="entry name" value="AdenylateSensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08587">
        <property type="entry name" value="UBA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103243">
        <property type="entry name" value="KA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="47"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA18053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="605" mass="69145" checksum="977E81D1524649DE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H276</accession>
    <name>A0A1A0H276_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">YTH-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709450.1">
        <property type="nucleotide sequence ID" value="XM_018856400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H276"/>
    <dbReference type="STRING" id="869754.A0A1A0H276"/>
    <dbReference type="GeneID" id="30029376"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21134">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04146">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50882">
        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YTH" evidence="1">
        <location>
            <begin position="138"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="31146" checksum="85CFCDFC77B7035E" modified="2016-10-05" version="1">MLLLFDSNLLVFKVPPRSRLKEPCTANTLRNLEIVTELANYTHGIDVVCTPNIWAPEYHDYFGEASRSVSETTIPTSATTPSSVSIFSSVSINSSNSQGWWPDDSDYFDLPDTAPSQPEFKRSFFRSEGKPFDVGESARFFVIKSYSSLDVQASIRNGIWASTELGNKRLDKAFRESQGGVYLFFSVNGSAKFCGVARMQSAIDFTQASDIWVESSRWKGIFPVEWLIVKEIPNRLLRHLRVYLNENKPVANSRDTQELPFDIGIAMMGTFDTYID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H2A5</accession>
    <name>A0A1A0H2A5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nuf2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_47714</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NUF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709452.1">
        <property type="nucleotide sequence ID" value="XM_018858206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H2A5"/>
    <dbReference type="STRING" id="869754.A0A1A0H2A5"/>
    <dbReference type="GeneID" id="30031182"/>
    <dbReference type="OrthoDB" id="8194677at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051383">
        <property type="term" value="P:kinetochore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041112">
        <property type="entry name" value="Nuf2_DHR10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18595">
        <property type="entry name" value="Nuf2_DHR10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="13">
        <location>
            <begin position="25"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Nuf2 DHR10-like" evidence="14">
        <location>
            <begin position="303"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="158"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="295"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA18057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="481" mass="54762" checksum="9C5D50B526896B1C" modified="2016-10-05" version="1">MSRLNATRPGAAYMAHSTRGHLVTNDEFPLLDFRELSQCLQECDFAASEELVARPSGSYIRKLFEQILDTFIGLSPDYCGGTARDLLQTPGPAGGADSGGADADDTADAVHVLVFFRAVNMFLQKCGILDFTLLDLVRPDPLRTQRVLSGVINFARFREEHLRECELLAKEAEEAMQEVRNLDDERVDLANRIDKLRRRLKEGDAAGAAAGGSAAAQKSLLAQLLKYNSKLEQELIKLQKSQEVFKKEHASYRETKARLIEKLEDQHFLLSESERELLKIEAYASADPAVVRQVVADLRLHSQAAEEELKKSETTVRNKTRTQQAVQTVEDELRNLIKIVQEISNDMKVLDAAKDNYARQNDELSSKQQISDELTVHLERAKRQFQKSEEKIAKLRQQALEKEQAAHEKFLALEMEYDMLTQDRDAKQEKLDRTKKENSQIEADIRKMKLDFDFEFRNTTSAVAKLNAHIRQYLEDISRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4W5</accession>
    <name>A0A1A0H4W5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_224077</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 12">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709525.1">
        <property type="nucleotide sequence ID" value="XM_018855163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4W5"/>
    <dbReference type="STRING" id="869754.A0A1A0H4W5"/>
    <dbReference type="GeneID" id="30028139"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10509">
        <property type="entry name" value="Zn-ribbon_RPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034014">
        <property type="entry name" value="Zn_ribbon_RPC11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="13">
        <location>
            <begin position="67"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA18990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="111" mass="12658" checksum="BBEF4E51FECB79C6" modified="2016-10-05" version="1">MLSFCPYCSNMLLISPGDDNCNSLYCPTCPYEFLVAGIQMYDRKVLPRKEVDDVLGGEGAWDNVDQTTAQCPIDSCGNDKAYFFQLQIRSADEPMTTFYKCTKCSHQWREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H571</accession>
    <name>A0A1A0H571_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Alkyl transferase</fullName>
            <ecNumber evidence="3">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_26169</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709635.1">
        <property type="nucleotide sequence ID" value="XM_018855311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H571"/>
    <dbReference type="STRING" id="869754.A0A1A0H571"/>
    <dbReference type="GeneID" id="30028287"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000005">
        <property type="entry name" value="Alkyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF2">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT SRT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="279" mass="32252" checksum="60DC66DF08A91335" modified="2016-10-05" version="1" fragment="single">FVRHKSIEILKQGRIPKHVGFIMDGNRRYAKEKGLFTKEGHKAGAEALKTALESCYEIGIKEISIYAFSIENFKRPKSEIDDILSLLRKFLESVYKTATVSEHNIQVRIIGNRSLIEPSLLEDLEKLESETKTSKVSAYLNVCFAYTSRDEITHSVREVVGKHQSKDITKNEICERLLTENLYTRDPSQPLDILLRTSGHTRLSDFLLWQCTSNCSIVFLDTLWPDFSYWELYLILLKWSFEQAVMRNRHLDWGFSTVPQYVNLKLLPDSPPFASVAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5A9</accession>
    <name>A0A1A0H5A9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mid2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_220433</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709753.1">
        <property type="nucleotide sequence ID" value="XM_018855135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5A9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5A9"/>
    <dbReference type="GeneID" id="30028111"/>
    <dbReference type="OrthoDB" id="4026433at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007567">
        <property type="entry name" value="Mid2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04478">
        <property type="entry name" value="Mid2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Mid2" evidence="3">
        <location>
            <begin position="194"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="308" mass="32130" checksum="BD880C0BA936BB3B" modified="2016-10-05" version="1">MAAISFLILAFVAVFGTILFTTQTCSFEKVHALMDQPHGNSTVLGYLARKPKYSEPQQQVLVRRATNLLLVDPGLATSSTSESLVVPSQEFTSIPTTAETSISSLVSSLSSSLSSSTLTPVTSSSLTSQTSTEGVTSNPLATTSTLSSIERSSVSSDIAPLTTESSSTSITSSTSSTSRTTSSSSSVSGSETTQVFSSVVSGRTVVVTQTSIVTQSTSEPSSSTSTDSTSSSSGLSDTNKIVVGVVVGVGGAILMAFAALMFILKRRKSKGHQSGWTFWRKNEKGGEDNFFSGELGVRDRDINQGSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Y6</accession>
    <name>A0A1A0H5Y6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="5">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF6</name>
        <name evidence="6" type="ORF">METBIDRAFT_13259</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <text evidence="5">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="PTM">
        <text evidence="5">Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710027.1">
        <property type="nucleotide sequence ID" value="XM_018854328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Y6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Y6"/>
    <dbReference type="GeneID" id="30027304"/>
    <dbReference type="OrthoDB" id="4155914at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.75.10.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine; by CK1" evidence="5">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="245" mass="26383" checksum="F814B87FA54F16A1" modified="2016-10-05" version="1">MASRTQFENSNEVGVFSKLTNSYCLVAVGGSENFYSAFEAELGDLIPIVRTTIAGTRIVGRMTAGNRRGLLVPVQTTDQELMHLRNSLPDTVKIQRVEERLSALGNVICCNDYVALVHPDIERETEELIADVLGVEVFRQTIAGNVLVGSYCSLSNQGGLVHPQTSIQDQEELSSLLQVPLVAGTVNRGSSVVGAGMVVNDWVAVSGLDTTAPELSVVESIFKLQDAQPDAISGNLRDTLIETYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Z8</accession>
    <name>A0A1A0H5Z8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein PNO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pre-rRNA-processing protein pno1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_46323</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709984.1">
        <property type="nucleotide sequence ID" value="XM_018858025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Z8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Z8"/>
    <dbReference type="GeneID" id="30031001"/>
    <dbReference type="OrthoDB" id="1932641at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042255">
        <property type="term" value="P:ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22392">
        <property type="entry name" value="KH-I_PNO1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000009">
        <property type="entry name" value="RNA-binding protein PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000048">
        <property type="entry name" value="RNA-binding protein PNO1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="10">
        <location>
            <begin position="163"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="258" mass="28565" checksum="AE003C13CD16AB69" modified="2016-10-05" version="1">MAAPTAIKNSAAAQPVQAPVENEEVLINTTGIPTQLEKPADNEDEEMKDDPQESSTLVLDESGKPRFAAASKSGMKVKLESRKVPVPPHRMTPLKNTWMKIYPPLVDHLKLQVRMNLKTKTVEMRTNKFTEDQGALQKGADFIKAFTLGFDVDDAIALLRLDDLYIETFEVKDVKTLTGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMAREAVVSLILGSPPGKVYGNLRTVASRMKERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H605</accession>
    <name>A0A1A0H605_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709880.1">
        <property type="nucleotide sequence ID" value="XM_018856320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H605"/>
    <dbReference type="GeneID" id="30029296"/>
    <dbReference type="OrthoDB" id="4068467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="632"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="634"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="670" mass="75105" checksum="957EC2577D61A27A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H677</accession>
    <name>A0A1A0H677_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Man(5)GlcNAc(2)-PP-dolichol translocation protein RFT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33111</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Intramembrane glycolipid transporter that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. RFT1 could mediate the translocation of the cytosolically oriented intermediate DolPP-GlcNAc2Man5, produced by ALG11, into the ER lumen where dolichol-linked oligosaccharides assembly continues. However, the intramembrane lipid transporter activity could not be confirmed in vitro.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the RFT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709946.1">
        <property type="nucleotide sequence ID" value="XM_018856226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H677"/>
    <dbReference type="STRING" id="869754.A0A1A0H677"/>
    <dbReference type="GeneID" id="30029202"/>
    <dbReference type="OrthoDB" id="9979195at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034202">
        <property type="term" value="F:glycolipid floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007594">
        <property type="entry name" value="RFT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MULTISPAN TRANSMEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13117:SF5">
        <property type="entry name" value="PROTEIN RFT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04506">
        <property type="entry name" value="Rft-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="356"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="481"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="517" mass="58639" checksum="AB37A6837714AFCA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H698</accession>
    <name>A0A1A0H698_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_214098</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709966.1">
        <property type="nucleotide sequence ID" value="XM_018855070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H698"/>
    <dbReference type="STRING" id="869754.A0A1A0H698"/>
    <dbReference type="GeneID" id="30028046"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="234" mass="26317" checksum="7B0C34E33E541526" modified="2016-10-05" version="1">MKESWFYLFSRPIQLAPLPAVIWGGIVYGAQMMWLSLMATTQSEIFETYYGFSTSSTGLTNLAPLAGCLIGMVYGGRFVDWLAVFLAKRNKGVLEAEFRLYAMWFPTLINAAGVLAYGLGANSKAPWAIPVIVGQGFLGFSMTSTGPICLTYAIECYPELSSEALVLMLFVRNMIGMGFTWGIQAWLERDGLVTTTWLMFMLSLIINGFAIFFIIWGKKWRKMTKEYYLRIANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6G4</accession>
    <name>A0A1A0H6G4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">OPT superfamily oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33308</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710202.1">
        <property type="nucleotide sequence ID" value="XM_018856267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6G4"/>
    <dbReference type="GeneID" id="30029243"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="288"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="485"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="509"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="670"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="711"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="748"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="792" mass="89552" checksum="77FA10B221193D25" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6I1</accession>
    <name>A0A1A0H6I1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Longevity assurance proteins LAG1/LAC1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_217898</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710169.1">
        <property type="nucleotide sequence ID" value="XM_018855105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6I1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6I1"/>
    <dbReference type="GeneID" id="30028081"/>
    <dbReference type="OrthoDB" id="537032at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005225">
        <property type="entry name" value="LAG1_LAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="9">
        <location>
            <begin position="110"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="336"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="350" mass="41019" checksum="F75BBCB5FB411451" modified="2016-10-05" version="1">MPSSQGFVVQFINRHQIAITRNLLAVLYLGDLLGSRLSLSFHKCASKFLHLQYLSDRLEGTSYYDIGINDCYHVVYWVVLLTFMRAVLMQYLFDPIAKHFFLVLSHKARVRFAEQSWSVVYYCFSFAFGLYLYYHSPYWNNIDNIFIGWPHYKMTALFKKYYLISIGFWLHQLVALNIEERRKDFVQMFFHHIVTCSLVIGSYYYYFNRIGNLILMIMDSVDILLSTAKILKYSSYTRACDAMFGLFVVAWVALRHGVYNYLFYISWHRSTELMLASECITGMLQKRCWNSYVINGFLSLLGGLQIISLVWLYMIGKVILRVIRGQNAEDVRSGTEDTDNEQEDSPADTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U2</accession>
    <name>A0A1A0H6U2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_16721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710339.1">
        <property type="nucleotide sequence ID" value="XM_018854669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U2"/>
    <dbReference type="GeneID" id="30027645"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF1">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="94"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="193" mass="21985" checksum="73FAEA5B668B7FFB" modified="2016-10-05" version="1" fragment="single">LYSHPVYEHYADIHREHLEQNPSSSSIQDTMQPNLTGHVLKGEDKINLRTQSLFVIDDKYLSEFDDIDGLLAGHKNHSYTFFHLGSHLSGHPDIVHGGLLATLLDEVSCRLAFQNIKSKKGVTANLNINYYKPCKTGYYIMLKCSVSKKLGRKCWVRGEVFHLKLDDEDFDIHKVETNENLISASECLVIEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H717</accession>
    <name>A0A1A0H717_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ras-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710419.1">
        <property type="nucleotide sequence ID" value="XM_018857819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H717"/>
    <dbReference type="STRING" id="869754.A0A1A0H717"/>
    <dbReference type="GeneID" id="30030795"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001767">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="213" mass="23065" checksum="7A20BB3CE29230BE" modified="2016-10-05" version="1">MNSIGNTTSVKLVLLGEAAVGKSSLVLRFVSNDFQENKEPTIGAAFLTQKCTVGDRTIKYEIWDTAGQERFASLAPMYYRNAQAALVVYDITKPASFIKARHWVKELHEQASKDITIALVGNKYDLVANDNDSEDSLRKVTVEEGQALAEEEGLLFFETSAKSSHNVNDVFVAIGSKIPEAKSTSPDVSVPSEGGRIDLSGNNLRSAASDCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H750</accession>
    <name>A0A1A0H750_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Trafficking protein particle complex subunit 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32947</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710449.1">
        <property type="nucleotide sequence ID" value="XM_018856171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H750"/>
    <dbReference type="STRING" id="869754.A0A1A0H750"/>
    <dbReference type="GeneID" id="30029147"/>
    <dbReference type="OrthoDB" id="10256906at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055504">
        <property type="entry name" value="DUF7076"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055505">
        <property type="entry name" value="DUF7077"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055506">
        <property type="entry name" value="DUF7078"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056916">
        <property type="entry name" value="NTS_TR130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23273">
        <property type="entry name" value="DUF7076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23274">
        <property type="entry name" value="DUF7077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23285">
        <property type="entry name" value="DUF7078"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24967">
        <property type="entry name" value="NTS_TR130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="5">
        <location>
            <begin position="13"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Trs130 NTS" evidence="9">
        <location>
            <begin position="345"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7076" evidence="6">
        <location>
            <begin position="602"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7077" evidence="7">
        <location>
            <begin position="755"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7078" evidence="8">
        <location>
            <begin position="957"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="4">
        <location>
            <begin position="1062"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1211" mass="139138" checksum="863FFAE348F1E322" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7E8</accession>
    <name>A0A1A0H7E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Myosin class V heavy chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710476.1">
        <property type="nucleotide sequence ID" value="XM_018857850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7E8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7E8"/>
    <dbReference type="GeneID" id="30030826"/>
    <dbReference type="OrthoDB" id="426293at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051020">
        <property type="term" value="F:GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15473">
        <property type="entry name" value="Myo5p-like_CBD_DIL_ANK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037986">
        <property type="entry name" value="Myo5p-like_CBD_DIL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dilute" evidence="1">
        <location>
            <begin position="326"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="825" mass="92820" checksum="B466C611717AEBF0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7J7</accession>
    <name>A0A1A0H7J7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Myo-inositol transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32910</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710392.1">
        <property type="nucleotide sequence ID" value="XM_018856160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7J7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7J7"/>
    <dbReference type="GeneID" id="30029136"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005366">
        <property type="term" value="F:myo-inositol:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904679">
        <property type="term" value="P:myo-inositol import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000073">
        <property type="entry name" value="MFS myo-inositol transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF12">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="53"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="540" mass="57579" checksum="D2054C637365021F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K0</accession>
    <name>A0A1A0H7K0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphatidate phosphatase APP1 catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12912</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710403.1">
        <property type="nucleotide sequence ID" value="XM_018854278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K0"/>
    <dbReference type="GeneID" id="30027254"/>
    <dbReference type="OrthoDB" id="541883at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019236">
        <property type="entry name" value="APP1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052935">
        <property type="entry name" value="Mg2+_PAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28208:SF3">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE APP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09949">
        <property type="entry name" value="APP1_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidate phosphatase APP1 catalytic" evidence="2">
        <location>
            <begin position="462"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="338"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="838" mass="91395" checksum="CC4A944416E8A47F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K8</accession>
    <name>A0A1A0H7K8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_210498</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710490.1">
        <property type="nucleotide sequence ID" value="XM_018855033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K8"/>
    <dbReference type="GeneID" id="30028009"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057072">
        <property type="entry name" value="bHLH_INO4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052207">
        <property type="entry name" value="Max-like/E-box_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15741:SF27">
        <property type="entry name" value="TRANSCRIPTION FACTOR AP-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23181">
        <property type="entry name" value="bHLH_INO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="7">
        <location>
            <begin position="13"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="101" mass="11617" checksum="83815980C3B0DF39" modified="2016-10-05" version="1">MDDITKASLTEEEKKAHHIASEQKRRENIRMEFDRIVELTPSLSSQESRSELNILTKSADYIDSLKEENARLLEVCNERGITVPEHLIYKGPGIAHEQAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7N1</accession>
    <name>A0A1A0H7N1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pex19 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_27153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710433.1">
        <property type="nucleotide sequence ID" value="XM_018855332.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7N1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7N1"/>
    <dbReference type="GeneID" id="30028308"/>
    <dbReference type="OrthoDB" id="21292at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033328">
        <property type="term" value="F:peroxisome membrane targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="282" mass="31243" checksum="00FD91144D1F8913" modified="2016-10-05" version="1" fragment="single">DDLDDLLDEFDEQVLSKPPGALVAESQDGLAAETDKNASATLNDEFKHNIEDLIKDLNIEDPEAKTQFEQLVKQFEESHMEEAKNAQTPQNFDNVMKDTMERLKKSGESIDEQMKKDPSAGNPEDMLTQLLAGLGDGSAGDGDLDMSKLLTDMLDQLSSKEVLYEPIRDLNAKFPEYLKENEGKISAEDHTNYTKQYEVTKEILAVFDAPGYDNNNLQHRDNVNSLLESLQELGNPPTELVGDENDFPGFGGGTGASSGLDFDSNDLPKDFENNLEEGCKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U9</accession>
    <name>A0A1A0H7U9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_212094</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710585.1">
        <property type="nucleotide sequence ID" value="XM_018855053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7U9"/>
    <dbReference type="GeneID" id="30028029"/>
    <dbReference type="OrthoDB" id="6910977at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="31365" checksum="06F2566970D055C9" modified="2016-10-05" version="1">MVDGVFTGNVAPCRLDQEQNKPDITHEHGTDQAAHLPIGLRNALTSSHRTARSSLDTRVKKFSHSFSASPRVTQAAEFAKPQAESFILNPWPVNTGVGPSANVSTAQEMNGALRVPLPCLSATDRWNKHTSKYTLSPKVSGDMICTHCDQKFTEVEEYTKHLDEKKVVHDNFCPDMSCAFAALGYRYRWLLRRHICNHHLKDYNNKKLRKNSSGKVTALTDNFLSHVYVCKKTNCLRAFYRLDSLLRHEKLIHSVCGKQKRKERLKLSKCDPKIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8A1</accession>
    <name>A0A1A0H8A1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710773.1">
        <property type="nucleotide sequence ID" value="XM_018857660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8A1"/>
    <dbReference type="GeneID" id="30030636"/>
    <dbReference type="OrthoDB" id="4077683at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="507" mass="58181" checksum="39B1BA0AF3E6AF78" modified="2016-10-05" version="1">MPKNSEPHPLSSAKCKRLVRPLLSKIHSLTDLYSKYPAKFVFDCELFISGSSEDAHFTKPATAEQRLVSLKPFLSLETYDAYVEIFNIFKNIVSTLYPDNAKTTKNGVPRLSFLAAHKLGKSIALGTKSTHYSLNQTALFDPASIPLYLRKYHDQLAEDIDEWLLMEPGSVLGQHRSQLLLGYVMHILVFNLRVLFYTLIPVLCHWLHEQKMQCLRTLFVEFWLFLYLDPDHEEVQDLTVPDKSRDPSLATFWLFHKIGYWQQMVRLLHIKSLVYSSAEAYSALLLEALICSDRLDLAHVDVHEVYALMRTNLQHPKNTQILVATIAQLITSFQKNYDVVSTSSEAHAAIFSAYLEIRNFLQAWVCLSKTLIFNTLDKGNTEIFDACSKFADHLKEICTLIIAYLESRSRGGSRSIERVRKFKKLLSEIGDLSGTCEVLRAFFLDIRLQSSEINASPTLVQYLASFTAEEYDRETLEVFMGWFEEQDQTKYGDFISLLQSHISAFEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8E6</accession>
    <name>A0A1A0H8E6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710818.1">
        <property type="nucleotide sequence ID" value="XM_018856025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8E6"/>
    <dbReference type="GeneID" id="30029001"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="90" mass="10712" checksum="2EC077FB9BDE2630" modified="2016-10-05" version="1">MTDAVNFFSQVVNVVNSAAKHCFSRFIWLKEQSYITMKNKKPLNSFQLRTKYRNSARLLPLFTALTIEVKNDLKYEMTQFLRRKRSTETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8G7</accession>
    <name>A0A1A0H8G7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Karyogamy protein KAR4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_43759</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MT-A70-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710838.1">
        <property type="nucleotide sequence ID" value="XM_018857694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8G7"/>
    <dbReference type="STRING" id="869754.A0A1A0H8G7"/>
    <dbReference type="GeneID" id="30030670"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51592">
        <property type="entry name" value="SAM_MTA70L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="346" mass="39401" checksum="94B3DDA06350791D" modified="2016-10-05" version="1">MSLTLGSSAHTSDVGAAQSTKRQRPIENDYSNNYLHTRALSTQYIRNIVRPTEGYPKLQKLKELKSQQVASHCTKPYGVHVETNQIVPTLNRWVNQYALQFDVIMIGALVENQFLLPLLNLLPLYKLCAKPGFLFIWTTTANIKQLTALLNGDKWNKKFRRSEELVFVPVDENSPYFPQGQNDGFYSDPSNGNCQSLLSRKQWHCWMCITGTVRRSTDTDLIHCNIDTDLQMDLKSKSAGDDGYNNAVPEAIYRVAENFSNSNRRLHIIPCKTGYRLPVKIRKGWVIMLPDVLVDNFDPVSYEMQLHANSMVKYKSINSSGMLKQVPQYLVPQTSEIEALRPKSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8L4</accession>
    <name>A0A1A0H8L4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_78678</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710749.1">
        <property type="nucleotide sequence ID" value="XM_018858917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8L4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8L4"/>
    <dbReference type="GeneID" id="30031893"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026028">
        <property type="entry name" value="V-type_ATPase_116kDa_su_euka"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF59">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A, GOLGI ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001293">
        <property type="entry name" value="ATP6V0A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="635"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="665"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="728"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="859"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="888"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="950" mass="106788" checksum="6445DBD8154ACBDB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8T9</accession>
    <name>A0A1A0H8T9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_43470</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710948.1">
        <property type="nucleotide sequence ID" value="XM_018857661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8T9"/>
    <dbReference type="STRING" id="869754.A0A1A0H8T9"/>
    <dbReference type="GeneID" id="30030637"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="3"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="439" mass="47324" checksum="7EA106EF5E69516B" modified="2016-10-05" version="1">MAEQIARDASGAFGILKTLSNEQRSHALKKIHDALAQAKTTVLEANKKDLADAENCRLSSALIKRLDLEKNGKYEAMLQGILDVASLPDPVGKTTLAKKLDEGLNLYRVLAPLGVLLIIFESRPEVIANISALAIKSGNCAILKGGKESYQTFKAISEVVNATLKHDTQVPELAIQLIQSRGEVSDLLAQDGYIDLVIPRGSNALVRNIKENTKIPVLGHADGICSIFIDKSADLDKACRVVVDSKTNYPAGCNAVEQLLIHEDIVKDSAKLDKLLASLTAAEVTLHVVPEINASLGESVDRKYVVDAAQNAFDIEYLSFDISVKPVADVSAAVAHINEHSSKHTDCIVTEDKPTADTFLKGVDSAGVYWNCSTRFADGFRYGFGTEVGISTNKIHARGPVGLEGLMSYQYQLKGDGHIALEYVGAGGSKVFVHEDIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8V2</accession>
    <name>A0A1A0H8V2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710835.1">
        <property type="nucleotide sequence ID" value="XM_018856030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8V2"/>
    <dbReference type="GeneID" id="30029006"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="72" mass="7985" checksum="AC8BBFC3E7ABBDF9" modified="2016-10-05" version="1">MLFTSARGAANIIPCYSHKTDPLNFQKSIIVGISKPISIYRPSHFTQTTILDPGYQKHQGYATSSFRLIGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H928</accession>
    <name>A0A1A0H928_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_78957</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711151.1">
        <property type="nucleotide sequence ID" value="XM_018858975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H928"/>
    <dbReference type="STRING" id="869754.A0A1A0H928"/>
    <dbReference type="GeneID" id="30031951"/>
    <dbReference type="OrthoDB" id="2019556at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015217">
        <property type="term" value="F:ADP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080122">
        <property type="term" value="F:AMP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005347">
        <property type="term" value="F:ATP transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015228">
        <property type="term" value="F:coenzyme A transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015230">
        <property type="term" value="F:FAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044610">
        <property type="term" value="F:FMN transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051724">
        <property type="term" value="F:NAD transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052217">
        <property type="entry name" value="Mito/Peroxisomal_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939:SF5">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45939">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PMP34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="112"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="212"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="324" mass="35239" checksum="5F9332C349894AE8" modified="2016-10-05" version="1">MSDDAELAHAIAGAGGGALSMIVTYPLITLSTLAQTTSKKKEAEDDQIEASSKCEIRKALLDKGGIKSLYAGLESALYGITLLNFIYYYFYELTSNVFLKSNAAAQRRKRGLSTLQLIITGAIAGAITVVGTNPFWVANTRMMTMAKEDGKSRSAFRALLEIAQKDGISTLFAGVLPALVLVINPIIQYTIFEQIKNMIVAQKGSRGFSAKKAFFIGAFGKLVATTLTYPYITLKSRMHVRKKSPGTDPSAAEQKADAQLSMYQEIKKIVAEEGMEGLYRGLAIKSFQSVTTAAFLFYFKEELFAGSLSLVGILRGRKMAKTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H935</accession>
    <name>A0A1A0H935_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_12408</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710914.1">
        <property type="nucleotide sequence ID" value="XM_018854226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H935"/>
    <dbReference type="STRING" id="869754.A0A1A0H935"/>
    <dbReference type="GeneID" id="30027202"/>
    <dbReference type="OrthoDB" id="2417308at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901235">
        <property type="term" value="F:(R)-carnitine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015220">
        <property type="term" value="F:choline transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034228">
        <property type="term" value="F:ethanolamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031460">
        <property type="term" value="P:glycine betaine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000046">
        <property type="entry name" value="Amino-acid permease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF7">
        <property type="entry name" value="CHOLINE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="528" mass="57683" checksum="D01BA94CF2CCBDE6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9D0</accession>
    <name>A0A1A0H9D0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_43345</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711148.1">
        <property type="nucleotide sequence ID" value="XM_018857647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9D0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9D0"/>
    <dbReference type="GeneID" id="30030623"/>
    <dbReference type="OrthoDB" id="2139348at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF31">
        <property type="entry name" value="MITOCHONDRIAL ORNITHINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="95"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="191"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="282" mass="30980" checksum="ECF2709001D2C551" modified="2016-10-05" version="1">MVNPTKEIAYGAAAGCLGKFIEFPFDTVKVRLQSAHGTRSTISVISHILRQEGVLGFYKGLKAPLFGACLESAILFSAYEYAQQFLRQLTPWLSSSMLSICCSGVFSGFAASFVLTPVELVKCNLQVANLLEKTRHESYGSILSQIIKRHGIAGLWLGLSSTLLREMAGTAVWFAAYEKSAIWYNRLWPEQKNINSLMSGATAGFLFNLSIFPVDTIKSNIQTAHISGRLAADLSFSSVLRSLVRRPGGVANLYNGLGITLVRSIPANAVIFYTYEYLKTHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9M4</accession>
    <name>A0A1A0H9M4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF3533 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_78926</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711109.1">
        <property type="nucleotide sequence ID" value="XM_018858969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9M4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9M4"/>
    <dbReference type="GeneID" id="30031945"/>
    <dbReference type="OrthoDB" id="2140105at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022703">
        <property type="entry name" value="DUF3533"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053001">
        <property type="entry name" value="MNNG_permease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34814:SF1">
        <property type="entry name" value="NITROSOGUANIDINE RESISTANCE PROTEIN SNG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12051">
        <property type="entry name" value="DUF3533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="102"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="487"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3533" evidence="3">
        <location>
            <begin position="111"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="532" mass="60241" checksum="BB9B8BAE57457287" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9U1</accession>
    <name>A0A1A0H9U1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_202328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711165.1">
        <property type="nucleotide sequence ID" value="XM_018854946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9U1"/>
    <dbReference type="GeneID" id="30027922"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="119" mass="12940" checksum="6E1F6CE5F385A02F" modified="2016-10-05" version="1">MAPASMRALCGATRASKKRDNGSAPWATRPRHYTARPQPTFLAGPQLFSPVPDFSRRTPILRNRQNPPGDSPGLRPLHRNLSGCPRWPASVPRAPARPAPGAVLHNAPSRPRPAHLARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAI0</accession>
    <name>A0A1A0HAI0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_165343</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711392.1">
        <property type="nucleotide sequence ID" value="XM_018854657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAI0"/>
    <dbReference type="GeneID" id="30027633"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="201" mass="22197" checksum="B7E022B567BDB572" modified="2016-10-05" version="1">MIFLMAFLMAFLMAFLMIFLKTFLKIFLMAFLKTFLKTFVVPTWRAFFCGSARRPVSGLRSSPSTPRPRLRWLGLGHCHVHLRRDLCNLSHCLLGAFDHDDPLLNAAAESRRYSAAPNLPPSTPVLAPSQMADECQVLGTPDTMTHKPRGTSYASATTSKRAGFTSGGSGSYWMTTADQMECPRSSASARSRRSGSKNTHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAK0</accession>
    <name>A0A1A0HAK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_41139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711412.1">
        <property type="nucleotide sequence ID" value="XM_018857361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAK0"/>
    <dbReference type="GeneID" id="30030337"/>
    <dbReference type="OrthoDB" id="4065597at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17242">
        <property type="entry name" value="DUF5315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="173" mass="19654" checksum="8BAD0BD17F43D91F" modified="2016-10-05" version="1">MITSTSDGSARSVLTVDRLPIARASVAALDKLWTQIDVLDDVKAMAEEVDRTGGFFTDDFSISLAQLKSSQSRLLEVISRHQALSDRAREQRRQLAKEHSELVSVDEEDILQQQERTRKRMSDFFSATAAPLVETAQSRDFDELNEYALEVRDSLGEVSEKMRDFDLVTKKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAS4</accession>
    <name>A0A1A0HAS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_42687</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711626.1">
        <property type="nucleotide sequence ID" value="XM_018857575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAS4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAS4"/>
    <dbReference type="GeneID" id="30030551"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="499"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="562" mass="65170" checksum="6A31FA3A8CF784CF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB02</accession>
    <name>A0A1A0HB02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_42155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711572.1">
        <property type="nucleotide sequence ID" value="XM_018857502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB02"/>
    <dbReference type="STRING" id="869754.A0A1A0HB02"/>
    <dbReference type="GeneID" id="30030478"/>
    <dbReference type="OrthoDB" id="194386at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF130">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EEF2KMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="33313" checksum="19DD7367BC8B6F7A" modified="2016-10-05" version="1">MAHDISAILDEQSQENVARDLAVIAQSNCFYVKLFLALYVRVLEQRSAVIDALYELYCEPRILSARELPPTAADTLQYSVDAGGETVAIRETPRVISGAGTTGLRTWEAALYMLNYLNNSASFSLISRGKRILELGTGTGLVLLALLKKHSQHQFASIALTDGDSALLENLPATFALNGLPPRVPVNVSQLIWGEELLYSEASGGPGRAPADIVVAADVTYDSLVVPSLCDTMAQFFQAGASVAYVAATIRNTATIEVWEAELDRRFSWTICDAVADPHGSELTCWFRRGTPEIRVYEIRGGRASGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBH2</accession>
    <name>A0A1A0HBH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_41075</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711845.1">
        <property type="nucleotide sequence ID" value="XM_018857349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBH2"/>
    <dbReference type="STRING" id="869754.A0A1A0HBH2"/>
    <dbReference type="GeneID" id="30030325"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005002">
        <property type="entry name" value="PMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10466:SF0">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03332">
        <property type="entry name" value="PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
        <property type="entry name" value="C2.B.3:_Phosphomannomutase_Lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01140">
        <property type="entry name" value="C2.B:_Phosphomannomutase_and_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="251" mass="28678" checksum="2783F3DBE4B9E5AA" modified="2016-10-05" version="1">MSFAHKEKPDTLVLFDVDGTLTPARLTISEEMKQTLQALRKKVVVGFVGGSDLSKQVEQLGPTVLQDFDYCFSENGLTAYKQGKELASQSFIGWIGEEKYNKLIKFVLRYLSELDLPVRRGTFVEFRNGMINISPVGRNASTQERNEFEKFDLEHKLRETMVQALRDNFPDYGLTYSIGGQISFDVFPTGWDKTYALQHVADEGFKEIHFFGDKSYKGGNDYEIYNDDRTIGHAVKEPADTIRILKELFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC05</accession>
    <name>A0A1A0HC05_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GPI-anchor transamidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_28186</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711917.1">
        <property type="nucleotide sequence ID" value="XM_018855348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC05"/>
    <dbReference type="STRING" id="869754.A0A1A0HC05"/>
    <dbReference type="GeneID" id="30028324"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000003">
        <property type="entry name" value="GPI-anchor transamidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500138">
        <property type="entry name" value="GPI8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019663">
        <property type="entry name" value="Legumain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR019663-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="34083" checksum="7735D3A5159001DB" modified="2016-10-05" version="1" fragment="single">TTEEAERVISSSGHTNNWAVLVLTSRFWFNYRHMANTLSLYRTVKRLGIPDSQIILMLADDIACNPRNAFPGTIFNNMDQAIDLYGDDIEVDYRGYEVTVENFIRLLTDRWGANQPRSKRLLTDENSNVFVYLTGHGGNEFLKFQDAEEIGAYDLADAFQQMHEKKRYREIFFMIDTCQANTMYERIYSPNILSVGSSRIDELSYSHHSDLEVGVAVIDRFTYYTLDFLEKIEKNSMVTMDKLFAEFTHENVHSNPGIRTDLFERDVSTVRLTDFFGNVQEVLVADVNEPLVEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCD4</accession>
    <name>A0A1A0HCD4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_190362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712170.1">
        <property type="nucleotide sequence ID" value="XM_018854830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCD4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCD4"/>
    <dbReference type="GeneID" id="30027806"/>
    <dbReference type="OrthoDB" id="2587563at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="540"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="619"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="781"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="652"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="827" mass="90676" checksum="BE44B15C20D7F951" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI7</accession>
    <name>A0A1A0HCI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_32120</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712143.1">
        <property type="nucleotide sequence ID" value="XM_018855985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCI7"/>
    <dbReference type="GeneID" id="30028961"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF43">
        <property type="entry name" value="PALMITOYLTRANSFERASE APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="213"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="169"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="360" mass="41466" checksum="9BCC5F95AF374901" modified="2016-10-05" version="1">MLESRARLPFVFRPGVSIIHKLVANWLLTDPNYVIDSDPSKLKNYQVQKHQDMGAWYLCGGRLRTGKKLPLNVIVALVMITCAVLFFIYEAPWTWSHLSPAVPVMFAYLWFLTAMFFVKAATLDPGIQPRNLHVPFDASLLERQNGPDEYFNTVSLPYFSDRVNGVTVKYCATCHIWRAPRMSHCAVCNSCVLVHDHHCVFLNNCVGYRNYRYFLWFLLTCVITSVYLATFTFLHCFWYRYSHVVEDGVLVNSFKASISQSPATFFLALAGCLASVYPLMLLGFHIFLTANNLTTREYLNYVRPLADTDDTYVNVYDRKSMMRNMWLTWVATPQCLSLLRPTEQYLDGDFTKERVPVLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCN6</accession>
    <name>A0A1A0HCN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Translation elongation factor eEF1 gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_30865</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712360.1">
        <property type="nucleotide sequence ID" value="XM_018855729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCN6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCN6"/>
    <dbReference type="GeneID" id="30028705"/>
    <dbReference type="OrthoDB" id="249703at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03181">
        <property type="entry name" value="GST_C_EF1Bgamma_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000006">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000142">
        <property type="entry name" value="Elongation factor 1 gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1010:FF:000001">
        <property type="entry name" value="Elongation factor 1-gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1010">
        <property type="entry name" value="Translation elongation factor EF1B, gamma chain, conserved domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050802">
        <property type="entry name" value="EF-GSTs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001662">
        <property type="entry name" value="EF1B_G_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036433">
        <property type="entry name" value="EF1B_G_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43986:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00647">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01183">
        <property type="entry name" value="EF1G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89942">
        <property type="entry name" value="eEF1-gamma domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50040">
        <property type="entry name" value="EF1G_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4 8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST C-terminal" evidence="7">
        <location>
            <begin position="82"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="EF-1-gamma C-terminal" evidence="6">
        <location>
            <begin position="246"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="408" mass="46208" checksum="37F892961D15A2E6" modified="2016-10-05" version="1">MSQGTLFADSKIRGAIPTALIKHFNLDVEIVEPESSAAFKKSFPLAKVPGFIGPKGVKLNEVIAISVYLLSLVEDHKLWGKNNTEYAEIFKWLSMTNSEFLPAAANVFKPLKGLMPYNKKQVDESRALLDKVTGIFEDRLSKYTFLVGERLTFADYFAAFLYVVAFKFLFGSEWRKQNPSITRWFKTVSSQALFKAALPEVEMCAKPIEYVPPKKEKKAAAPAAKKEAPKKEAAPAEAAPTEAPKPKHPLELLGKPKQALDEWKRIYSNEETRETAIPWFWKNMYDPSEWSLYKVDYKYNDELTLTFMSNNLVGGFFNRLSASTKYMFGCMVVYGENNNNGITGFFLVRGQDFAPAFDVAPDWESYAYTKLDGSDENVRKFVDNMLAWDEPVEVNGEKVEIADGKVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS2</accession>
    <name>A0A1A0HCS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_32161</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712197.1">
        <property type="nucleotide sequence ID" value="XM_018855993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCS2"/>
    <dbReference type="GeneID" id="30028969"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140727">
        <property type="term" value="P:siRNA-mediated pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 23" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="38"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="216"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="478"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="577"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="677"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="760"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1133"/>
            <end position="1225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA21687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1234" mass="140072" checksum="B763CE40A25CF2F5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCU5</accession>
    <name>A0A1A0HCU5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Actin depolymerizing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_32210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the actin-binding proteins ADF family. GMF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712258.1">
        <property type="nucleotide sequence ID" value="XM_018856009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCU5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCU5"/>
    <dbReference type="GeneID" id="30028985"/>
    <dbReference type="OrthoDB" id="3919494at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071933">
        <property type="term" value="F:Arp2/3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071846">
        <property type="term" value="P:actin filament debranching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034316">
        <property type="term" value="P:negative regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011171">
        <property type="entry name" value="GMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249:SF2">
        <property type="entry name" value="GLIA MATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11249">
        <property type="entry name" value="GLIAL FACTOR NATURATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001788">
        <property type="entry name" value="GMF-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="3">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="109" mass="12479" checksum="10F1F0E68B465C7F" modified="2016-10-05" version="1">MQAVIYVIDKQSYQVVAESKGPVTSIEDLIEELPDNTPRYVVLSYPMKLLDGRLKSPLVLVYWRPPTCGQESKMLYAGAVELMREKAGVSQLIEIDDEEDFEDIETRFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD23</accession>
    <name>A0A1A0HD23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HIRA-interacting protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_143747</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712480.1">
        <property type="nucleotide sequence ID" value="XM_018854502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD23"/>
    <dbReference type="STRING" id="869754.A0A1A0HD23"/>
    <dbReference type="GeneID" id="30027478"/>
    <dbReference type="OrthoDB" id="565552at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000001">
        <property type="entry name" value="NFU1 iron-sulfur cluster scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.70">
        <property type="entry name" value="Scaffold protein Nfu/NifU, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014824">
        <property type="entry name" value="Nfu/NifU_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036498">
        <property type="entry name" value="Nfu/NifU_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035433">
        <property type="entry name" value="NFU1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001075">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178">
        <property type="entry name" value="IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11178:SF1">
        <property type="entry name" value="NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08712">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01106">
        <property type="entry name" value="NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036773">
        <property type="entry name" value="HIRIP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00932">
        <property type="entry name" value="Nfu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110836">
        <property type="entry name" value="Hypothetical protein SAV1430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffold protein Nfu/NifU N-terminal" evidence="3">
        <location>
            <begin position="34"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="201"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="252" mass="28049" checksum="6EEB7645D699B366" modified="2016-10-05" version="1">MFSRSLLNTLLKAVLPRVSPRVSLPLSWSRNLFIQTADTPNENALKFLPSMKILGESETMEFLSGREAACSPLAMKLFSVDGVKSIMLGPNFITIEKVDESFEWALLKPEIFSILTEGLTNGTPILDGSELSKDIGFDEEDDEVISMIKELIFTRIRPAIQDDGGDIEFVNFVEETGTVFLRLKGACRTCDSSSVTLKNGIESMLKHYIEEVEEVKQVEEEVEEADESFAKFEAAKIVPQSREDSSTLPPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD24</accession>
    <name>A0A1A0HD24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNase I-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712339.1">
        <property type="nucleotide sequence ID" value="XM_018853849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD24"/>
    <dbReference type="STRING" id="869754.A0A1A0HD24"/>
    <dbReference type="GeneID" id="30026825"/>
    <dbReference type="OrthoDB" id="62798at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF275">
        <property type="entry name" value="LD06095P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="419" mass="46712" checksum="B09C97B26D6DA360" modified="2016-10-05" version="1">MSIPLYLLTLNCGKLQINTADFADNLQQSLPDAPSDLFVFGFQEICSIMDGTFAPSARKHMFDINRIVLHALNTKYNGPADADLAFTTLGVHHVGVIGMILVTPFALKFGTCRFADASCGNAKSLLKGAVGIRVSYSAADSLLTELTFATAHLSAFEGSFYYERRIQNIQTLMRALDFGDGFSFLKPGSHAFFMGDLNFRTTQDPKNYEAALGELVQLQDNTKQLEPNIVPSLVAKYDELTRGRTEGELFSGFDEPCITFHPTYKFHLNTAIYNSKRCPLWCDRILYQSTYKKGKVPVIHEYNSASTYLRSDHRPVYLKITVPLEAPESIIAENGYFVVLPAASHLAHNSQLEGFDPNTAVSGPTQVYMKFTALDRLHQYAFRRIADLGIGYGLWLATTTRGRLVVFVLALMAWLLYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD82</accession>
    <name>A0A1A0HD82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31008</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712535.1">
        <property type="nucleotide sequence ID" value="XM_018855751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD82"/>
    <dbReference type="STRING" id="869754.A0A1A0HD82"/>
    <dbReference type="GeneID" id="30028727"/>
    <dbReference type="OrthoDB" id="6133115at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF55">
        <property type="entry name" value="SUGAR TRANSPORTER STL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="21"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="509"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="514"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="541"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="60649" checksum="68637B2D2A000338" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD90</accession>
    <name>A0A1A0HD90_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712394.1">
        <property type="nucleotide sequence ID" value="XM_018855733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD90"/>
    <dbReference type="GeneID" id="30028709"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="134" mass="15386" checksum="ECE64ED80EC11A30" modified="2016-10-05" version="1">MHHIIYIAISPRDIELELPRAPNLFWDNHHRPNTLYNKKQTEKIPPSELQVNGGKRSICPAPDSPGSPGPAAPPHSPYSHQSWDRPRLRQITRHQKPITAHTPKPRSPARTEEPNQKQPNKKQNENEKTKSKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDC3</accession>
    <name>A0A1A0HDC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating</fullName>
            <ecNumber evidence="13">1.1.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">C-3 Sterol dehydrogenase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 17">C-4 decarboxylase ERG26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sterol-4-alpha-carboxylate 3-dehydrogenase erg26, decarboxylating</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_30988</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>4beta-methylzymosterol-4alpha-carboxylate + NADP(+) = 3-dehydro-4-methylzymosterol + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:33447"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:50593"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64925"/>
            <dbReference type="EC" id="1.1.1.170"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33448"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of ERG25, ERG26, ERG27 and ERG28. ERG28 acts as a scaffold to tether ERG27 and other 4,4-demethylation-related enzymes, forming a demethylation enzyme complex, in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 3-beta-HSD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.170" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712512.1">
        <property type="nucleotide sequence ID" value="XM_018855748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDC3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDC3"/>
    <dbReference type="GeneID" id="30028724"/>
    <dbReference type="OrthoDB" id="10058185at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000252">
        <property type="term" value="F:3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000346">
        <property type="entry name" value="C-3 sterol dehydrogenase/C-4 decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002225">
        <property type="entry name" value="3Beta_OHSteriod_DH/Estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050177">
        <property type="entry name" value="Lipid_A_modif_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245">
        <property type="entry name" value="BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN ARNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43245:SF51">
        <property type="entry name" value="SHORT CHAIN DEHYDROGENASE_REDUCTASE FAMILY 42E, MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01073">
        <property type="entry name" value="3Beta_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291750" description="Sterol-4-alpha-carboxylate 3-dehydrogenase ERG26, decarboxylating" evidence="20">
        <location>
            <begin position="24"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="3-beta hydroxysteroid dehydrogenase/isomerase" evidence="21">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00082106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA22016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="350" mass="38602" checksum="EE19FA09CD16EB33" modified="2016-10-05" version="1">MAPLKSVLLVGGAGFLGLHLVQQFYTHCPDTQIHVFDIRPIPAEISKYFTFDRLKVTFHQGDLTLEKDVLAAINASKCEVVVHSASPIHGMAQEIYHKVNVVGTKTLLKVARDSPVQALVYTSSAGVIFNGADVFNANESWPYPAKHMDGYNETKAAAEEAVMAANEPGQLLTVCLRPAGIFGPGDRQLVPGLRTAARLGQLKFQLGDNDNLFDWTYVGNVADAHVLAAERILDAQHAPRLGGETFFITNDAPTYFWTLARTVWKADGHIDSHNIKLHYPVAIVAGYLSLFVSKLLNKEPGLTPFRVKITCATRYHDITKAKQVLGYEPAVSLEDGIQHTLDWIYETHKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK4</accession>
    <name>A0A1A0HDK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ras GTPase-activating-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_31051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712592.1">
        <property type="nucleotide sequence ID" value="XM_018855762.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HDK4"/>
    <dbReference type="GeneID" id="30028738"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903479">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21206">
        <property type="entry name" value="CH_IQGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12206">
        <property type="entry name" value="RasGAP_IQGAP_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF14">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143885">
        <property type="entry name" value="RGC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50018">
        <property type="entry name" value="RAS_GTPASE_ACTIV_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="131"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GAP" evidence="2">
        <location>
            <begin position="908"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1565" mass="177918" checksum="2C5B472ABC30BB19" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDR2</accession>
    <name>A0A1A0HDR2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF171-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31161</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712720.1">
        <property type="nucleotide sequence ID" value="XM_018855784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDR2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDR2"/>
    <dbReference type="GeneID" id="30028760"/>
    <dbReference type="OrthoDB" id="361029at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003750">
        <property type="entry name" value="Put_MeTrfase-C9orf114-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150">
        <property type="entry name" value="CLASS IV SAM-BINDING METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12150:SF13">
        <property type="entry name" value="METHYLTRANSFERASE C9ORF114-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02598">
        <property type="entry name" value="Methyltrn_RNA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="367" mass="40665" checksum="7BD82668146CE84C" modified="2016-10-05" version="1">MVSNLPTNSGEESTNPSNDVKISLCIPSSVISQSNASNLEHITHVAYQIAKAATIYNVVEIVTLEIPSKRDRQDKASDAKESQISLPGDNGNKKIKFNSVAEEKTQSDFQEQTNEGGNDTTDKNNENNALLLASLLQYFVTPKYLVKSVFRNSPHVKKFKYAEKLPTLTALPFMKNEGVRKAFKEGFTVAKQAPKIRKKSKKSSAVKKLRVTKYVNIGDSKLFELSGPEVPTNVRVTVDLKNKRVVSPESAYAMTACKASYGYHVRLAKTVTSVFTELSHADGYTESIFVNADNFFGDISEVHIPEKKNDWQGQILLVIGSLNDLEYVFNQESIDEIDSIAEMFDGQMIVPPGLRIEDAAFVSLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDT3</accession>
    <name>A0A1A0HDT3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_67792</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712745.1">
        <property type="nucleotide sequence ID" value="XM_018858368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDT3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDT3"/>
    <dbReference type="GeneID" id="30031344"/>
    <dbReference type="OrthoDB" id="9936937at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058841">
        <property type="entry name" value="HTH_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040554">
        <property type="entry name" value="KPWE_PEX14_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855">
        <property type="entry name" value="CHROMOSOME 10, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36855:SF1">
        <property type="entry name" value="PEROXISOME MEMBRANE ANCHOR PROTEIN PEX14P N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25871">
        <property type="entry name" value="HTH_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17733">
        <property type="entry name" value="KPWE_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PEX14-like helix-turn-helix" evidence="3">
        <location>
            <begin position="6"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Peroxisomal membrane protein PEX14-like KPWE" evidence="2">
        <location>
            <begin position="111"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="173" mass="19374" checksum="2A244579FB0F0D5D" modified="2016-10-05" version="1">MADPQERVYAEFSTFDWSSFTEFKEGLGEILQGHLESLQERDPSVKTIPAAEKQQLTDQAKSFFFCSSTGHILNLDDYYAWKRNNGGKITLLEESSKEEDVSDDKDSSEAPYSSNYQELVDLIVSGKPVPGIKEIPDTVLSDKSSEPKAEKRVKPWEKCAAANKPNVLDMTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDY3</accession>
    <name>A0A1A0HDY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_40341</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MATALPHA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712686.1">
        <property type="nucleotide sequence ID" value="XM_018857261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDY3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDY3"/>
    <dbReference type="GeneID" id="30030237"/>
    <dbReference type="OrthoDB" id="4095637at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045895">
        <property type="term" value="P:positive regulation of mating-type specific transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006856">
        <property type="entry name" value="MATalpha_HMGbox"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04769">
        <property type="entry name" value="MATalpha_HMGbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51325">
        <property type="entry name" value="ALPHA_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Alpha box" evidence="6">
        <location>
            <begin position="66"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="176" mass="20255" checksum="11AA8DDB66C14AD1" modified="2016-10-05" version="1">MEKSPFCDPNLASQKATYSVLQSRALPQIPIPSSSLQDLLCKYLFNEVDTNKKLSRIKQGTRYNDTKKRRINGFIAFRSFYSKTIKGTSHQKELSSKLAAIWKTEPNHQTWNSYALQYNATGGNESFVSWLNRKLGFDTNIEAKKTFKSYKNLTLKNVEDVFFEDINYDLNGQTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE16</accession>
    <name>A0A1A0HE16_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Phosphomevalonate kinase</fullName>
            <ecNumber evidence="3 13">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_77708</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP</text>
            <dbReference type="Rhea" id="RHEA:16341"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16342"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="3 13"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712752.1">
        <property type="nucleotide sequence ID" value="XM_018858747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE16"/>
    <dbReference type="STRING" id="869754.A0A1A0HE16"/>
    <dbReference type="GeneID" id="30031723"/>
    <dbReference type="OrthoDB" id="10262935at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031388">
        <property type="term" value="P:organic acid phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016005">
        <property type="entry name" value="Erg8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017288">
        <property type="entry name" value="PMK_GHMP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="14">
        <location>
            <begin position="332"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="431" mass="47493" checksum="0A1A86402C79CDA1" modified="2016-10-05" version="1">MTRVFGAPGKALVAGGYLVLDPEYNSYVTALSSRMHARIQLDPVTDTYSKITVSSPQFNGFWEYTIAHDTGFFKTKTRIGGRNPFIEETVKVLLAYVEPQKLFNFQITLFSDPGFHTQDDTEQRYSDNSKKMFLYHKKPIEQVSKTGMGLSAGLVSVVSAALLATFLAKPASDIIHIIHNVSQIAHCGAQGKIGSGFDVAAAVYGSIIYRRFSPMVISEILDQQFSSEYCASIKKIVNTEWDFVHSSCSLPPGLRLIMGDVNGGSETPRLVSQVLNWRNKDEDSVSVYETLNLANCRFMAELQKLNEFSSQNPSKYKQELEAGTLFMNLSNCIAAIREGLQLLTSKSGADIEPPEQKHLLDKCEELPGCLGGVVPGAGGYDAICLLVDSSQVANLKQTTRLDESFASVTWLELTEESQGIVEELAEDYFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE45</accession>
    <name>A0A1A0HE45_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_147884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712668.1">
        <property type="nucleotide sequence ID" value="XM_018854542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE45"/>
    <dbReference type="GeneID" id="30027518"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="10375" checksum="A87DFE9564552507" modified="2016-10-05" version="1">MLRERATNCATRPLLRFQTLFKTIFINIIILFMFQSLQIRIHSCTCKLTIESHYFLFEQKIHWYVAGSCLKLFYIYILYSFAPSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE77</accession>
    <name>A0A1A0HE77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_68148</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712910.1">
        <property type="nucleotide sequence ID" value="XM_018858369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE77"/>
    <dbReference type="STRING" id="869754.A0A1A0HE77"/>
    <dbReference type="GeneID" id="30031345"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF25">
        <property type="entry name" value="FERRIC_CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291763" description="FAD-binding FR-type domain-containing protein" evidence="10">
        <location>
            <begin position="17"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="247"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="323"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="11">
        <location>
            <begin position="383"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="660" mass="75503" checksum="E1C56808C01F3570" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEJ8</accession>
    <name>A0A1A0HEJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_38877</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712906.1">
        <property type="nucleotide sequence ID" value="XM_018857062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEJ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEJ8"/>
    <dbReference type="GeneID" id="30030038"/>
    <dbReference type="OrthoDB" id="200187at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071256">
        <property type="term" value="C:translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011553">
        <property type="entry name" value="Sec62_asco"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00869">
        <property type="entry name" value="sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="186"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="268"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="291" mass="32638" checksum="03A84B004BBE839B" modified="2016-10-05" version="1">MAANNPAVGAHGPAAGETQVPLNQERSPVIMNVANYLRGNSMLKMRGGLLNNKEEVDFFRFKRMKRALLSDDYKKKSADGKNQLVLIPNEQEVAKMFVQFILARLLVPVEKLHYHEIKAVKGWVPNRQKPTLRPTTKATLDPDAYYAWTYQKPNPYLVLYGFLMIAAVFAVILFPLWPVFMRIGVWYLSMGCLGLLGLFFAMAIVRLIIFVVTFVALPHAFWLYPNLFADCGFFESFQPLYAWSGPSEKKSKKKRAAAVTQPEAVAQATASGVQKSSGAQGRTVTLEEVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HET0</accession>
    <name>A0A1A0HET0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_40428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712976.1">
        <property type="nucleotide sequence ID" value="XM_018857272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HET0"/>
    <dbReference type="STRING" id="869754.A0A1A0HET0"/>
    <dbReference type="GeneID" id="30030248"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000191">
        <property type="entry name" value="Methylglyoxal reductase (NADPH-dependent)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="8"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="37905" checksum="83560C1CBF59ACCC" modified="2016-10-05" version="1">MTTTKICVFVSGASGFIAQHTVKLLLEKGYAVVGSVRSKAKGKRLTGLFKSTDFRYCVVPDIEPAGAFDEAVKSHPEVTAFLHTASPFHYNATDVEQELLLPAVNGTRNALCAIKKYGPQIEHVVITSSYVAMWDIDKFYDPTHREDEELWSPITWEEARVDAQQAYYGSKKFAEKAVWEFIETQSPKFTVNFVNPVLVFGPQAFDAEVTPELNTSSEIVNGLLKLDAESQVPEIASVFVDVRDVARAHLVAFESKMPNVRLLLYASKFTAQGIMDIIKQTADLLMESLPMGNPGSGPCAEAKICHIDNSKTRALLGFKLINLRTSVVDTVAQIVRARNALGST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF21</accession>
    <name>A0A1A0HF21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77968</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713097.1">
        <property type="nucleotide sequence ID" value="XM_018858796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF21"/>
    <dbReference type="STRING" id="869754.A0A1A0HF21"/>
    <dbReference type="GeneID" id="30031772"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF564">
        <property type="entry name" value="STEROL-4-ALPHA-CARBOXYLATE 3-DEHYDROGENASE, DECARBOXYLATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="3">
        <location>
            <begin position="5"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="38300" checksum="2DD6338FB29FE482" modified="2016-10-05" version="1">MSTTVFVSGATGFVGQHLVQLLIAKKYHVVGTVETIEQGNHLQRLFNSKNFTYEIVKATGVPNPFANAFKRHPEITVFIQTQQPRTHEEPSVKTDMLNVLHAIKDYAPQIEHVVVTSTAASFIDTGSLYDSACTICEGPWSPVTCDHAENLQTLGCLGFTEKTAWDFYDNTSPTYTLSVISPAHVFGPQAFDVGFEEKLNSVEIISDILSMTPESEVPLISGQFIDARDLARAYLVILERRFSHKRFLLSSESFSGQAILDILRQNFYSLRNELPEGTPGEGKSESQAGNSTIREILGDLIIDLHTSVVDSVNQIMRAHKKEYCPPGMFLQKGHRVFRAPTTFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF62</accession>
    <name>A0A1A0HF62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_39342</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713028.1">
        <property type="nucleotide sequence ID" value="XM_018857130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF62"/>
    <dbReference type="STRING" id="869754.A0A1A0HF62"/>
    <dbReference type="GeneID" id="30030106"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="UniPathway" id="UPA00661"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000018">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000015">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF352">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 14" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="17">
        <location>
            <begin position="34"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="16">
        <location>
            <begin position="157"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="18">
        <location>
            <begin position="296"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="15">
        <location>
            <begin position="510"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA22532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="707" mass="78411" checksum="1BBE49BA92664712" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF98</accession>
    <name>A0A1A0HF98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_28487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713295.1">
        <property type="nucleotide sequence ID" value="XM_018855357.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF98"/>
    <dbReference type="STRING" id="869754.A0A1A0HF98"/>
    <dbReference type="GeneID" id="30028333"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="233"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="328" mass="35117" checksum="2B114789BC142507" modified="2016-10-05" version="1" fragment="single">VSVVSFSVPADDTLLVAAYDRSVRLYNCKRRSTTASLAAPAPVLCVAYSPYRTSFAGLLDGTIRQLDYENLAVSAPVVSAAGRADEGSGTGNGINCFRPLDTSLFVATTFGGTMLRFDPRALRVAHTQQLRGKALAMDTCGHLVTVALLQQAVEIHDVRKWGAPVLTRASGLRFQALALRAFPSGEGYVLSSVDGRVSVEYYDDLAAAQTQKFAFKCHRVRAPAGGEDAVYPVTGLRFHPVHGTLFTAGGDGNVCVWNWEKRKRMKLLAHGLELPRAISHMDLSADGAYVAVSVDDDSYSRTGGAQAAARGGRVLVRPLGDAECKPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFA9</accession>
    <name>A0A1A0HFA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Nop domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37361</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRP31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713162.1">
        <property type="nucleotide sequence ID" value="XM_018856865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFA9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFA9"/>
    <dbReference type="GeneID" id="30029841"/>
    <dbReference type="OrthoDB" id="4771285at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027105">
        <property type="entry name" value="Prp31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019175">
        <property type="entry name" value="Prp31_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13904:SF0">
        <property type="entry name" value="U4_U6 SMALL NUCLEAR RIBONUCLEOPROTEIN PRP31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09785">
        <property type="entry name" value="Prp31_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="187"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="442" mass="48617" checksum="41EE0C35CEA11103" modified="2016-10-05" version="1">MDALSALGALDTSGALGLSQVQALIPVIRADLEKYADLMETEYMDLVASIHQQDQSAEYRFLVQLSDLPVLVHDEMLALRRFVALRYQVVFPELELLVASPVDYCRVVLEIGQDLAGVRAHEPRLRQIVAADKVLTIVMAALQQFPQAFQLSPGDFAPIAAACHACLELSGFLQEISQFVAGKLGKYAPNVAQVVGPVATAQLLVSTGSLAQLAQTPACNLAALGVRELLSQHKARLAPWVRAPGYLYQSPLVAGLPPEIVRQALRVISAKVVLAARIDLSRSSPDGGMGRAYRDEIRRKIDQLLAPPELTAARALPVPKEQKLKKRGGRRFRKMKERFQMSDLRKAQNKMLFGTQEQTVMDGFGDEVGLGMSRQMDGVLVNRNTDARMSKAMVARLQQQKQRETLDTIVLAPVAEKRARAPDVPGKWGTMKRQRLDGEDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFB4</accession>
    <name>A0A1A0HFB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_75891</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713167.1">
        <property type="nucleotide sequence ID" value="XM_018858438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFB4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFB4"/>
    <dbReference type="GeneID" id="30031414"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF17">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="3"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="505" mass="55854" checksum="B627165EBE231705" modified="2016-10-05" version="1">MSHLLVIALSSGLGTGLLVGAADKLRVGGPLALLLAYAIVGLMCICTMNSVGELTVAYTELEGGFNDWYRKFLDESVAFAFAWTYYFQWTVAISLELVTAAMIIKFWNTRINSDVFVAVFLVAIVAINFFGVRGYAHAELVMNLAKLLMLTGFVLMGLCIDLGASRSGFIGGLYWHDPGFFVSFKGFVQCFATASFSMGGTEFLALSVSDVRNPRRALPVAIRLVFVRIVFFYLGSLVFVGLLVPYDSLRLLGASALATSASPYVLAASLHGVPVVPHIINAVILNSVMSVATAAMYSLARLLRSLAQQGFAPRWLDYTDRAGRPLRAWLVSVVLSCLSFIATYENQAVVFNWLLSIVALALVYIWSGLCVCHLRWRAALRHQGIPLATLGFVSYTGEWGSYYSLLINFLILIGQFWVALFPGSTPDANSFFQNYLAIPFTLVLYLAHKTYAGTWRTPYIKIADIDLVTGRSVYDADVLALDREEKQQKLLSLRWFAKPFVWFFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM9</accession>
    <name>A0A1A0HFM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="3">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_76104</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713440.1">
        <property type="nucleotide sequence ID" value="XM_018858477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFM9"/>
    <dbReference type="GeneID" id="30031453"/>
    <dbReference type="OrthoDB" id="238681at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1670.10:FF:000005">
        <property type="entry name" value="N-glycosylase/DNA lyase OGG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="13">
        <location>
            <begin position="134"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="39544" checksum="E5D584EFC8CAA69D" modified="2016-10-05" version="1">MPADVLWKSMPLSPKELSLARVLRCGQAFRWKNVNNVWSVSTQDQIVLLRQDDAALHYAAIGPETAGAGPPPHAASHFVRDYFNLHVQLEALYRAWAEVEARFSNGLKRAAFGLFPGIRMLRQEPWETVVLFICSSNNNVKRISKMCDAVCAEYGTYINEHGGVRYYSFPGPDALAAAGVEGRLRELGFGYRARYIHQTAQMFVDAARPEISVAALYELRNRLYEEAHGFLLRLTGVGPKVADCICLMALDKHDVVPVDTHVMQIAVRDYKFRGPRAMTQKAYHQVRGHLRSLFGDYAGWAQLVMFAADLSDLNNGINQLDGAPVRHEVKGEASVRTGTEVETSVKPAKRVKSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ1</accession>
    <name>A0A1A0HFQ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_96033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713465.1">
        <property type="nucleotide sequence ID" value="XM_018859419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ1"/>
    <dbReference type="GeneID" id="30032394"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="94" mass="10531" checksum="267003013796B491" modified="2016-10-05" version="1">MLWVVPIYYLRAHFQALSRATNALGLPYLAVPLQIPSDHFAALAPSGVYSVKAAFPAEHSSYVIYDDTFTFVHTHNAIFNHVLHNMVSPLQEYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ3</accession>
    <name>A0A1A0HFQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61</fullName>
            <ecNumber evidence="2 11">2.1.1.220</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_35999</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine(58) in tRNA + S-adenosyl-L-methionine = N(1)-methyladenosine(58) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10365"/>
            <dbReference type="Rhea" id="RHEA-COMP:10366"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.220"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer; composed of two copies of TRM6 and two copies of TRM61.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.220" evidence="2 11"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713312.1">
        <property type="nucleotide sequence ID" value="XM_018856680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFQ3"/>
    <dbReference type="GeneID" id="30029656"/>
    <dbReference type="OrthoDB" id="1925287at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.20:FF:000002">
        <property type="entry name" value="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF2">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="14">
        <location>
            <begin position="64"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="295"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR017269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="370" mass="41763" checksum="4156CFB48A42FA4C" modified="2016-10-05" version="1">MSFYHYKDYIEEGDMVLAFISRGLLKPLTVTKGEFLNTRFGSFDHDRMIGMKYGSQMGGIKDKGFVHLLHPTPELWTVSLPHRTQIVYTPDSSYIVQRLGISSGSRVIEAGTGSASFTHAFARTVSHEGKVFTYEFHEPRYLEAKRELGQHGLLQENCVITHRDVCNDGFDIAGLPDTDLVEGRLSCDAVFLDLPSPWTAIPNLKSVIAADRRVGICCFSPCIEQVDKTIEALEKNDWTDIEMVEIAGRRWEARKEMERHVQDVIKRLKHIQGRKSQGIESRRLVRVELASTAGEKRPLSEVEQDSALPEPHGSTGPSFNPFGKGLRIREGDEQFNWKQVTKIESEVKTHTSYLTFAYHVPSKQDGVPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFX3</accession>
    <name>A0A1A0HFX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ARM repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_29599</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713540.1">
        <property type="nucleotide sequence ID" value="XM_018855445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFX3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFX3"/>
    <dbReference type="GeneID" id="30028421"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1032" mass="112986" checksum="89DAD22D41F9CF0B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY7</accession>
    <name>A0A1A0HFY7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphotransferase</fullName>
            <ecNumber evidence="8">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_36346</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the hexokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713397.1">
        <property type="nucleotide sequence ID" value="XM_018856725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY7"/>
    <dbReference type="GeneID" id="30029701"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00180"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019158">
        <property type="term" value="F:mannokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000034">
        <property type="entry name" value="Phosphotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019807">
        <property type="entry name" value="Hexokinase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022673">
        <property type="entry name" value="Hexokinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF30">
        <property type="entry name" value="GLUCOKINASE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03727">
        <property type="entry name" value="Hexokinase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00378">
        <property type="entry name" value="HEXOKINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Hexokinase C-terminal" evidence="10">
        <location>
            <begin position="223"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="477" mass="53049" checksum="6AF4956FA7741120" modified="2016-10-05" version="1">MCSLNTDLQKAIADIEQLFEVDKAFLCKATDHFVKCMDAGLTNEVPNRVYMPMLPTFVTSIPTGKENGLFLAVDLGGTNFRVCSIDLKGDHTFELKQSKFPVPIDLMRGSTSDLLFSFFAEKVKNFLEEHHEELDESITLKMGFTFSFPVDQTAIDSGKLIRWTKGFDLPDCIGCDVVKYFQKHLDLLNLPVMITALANDTVGTLLSRAYTNDRSASKSRTLIGAIFGTGTNGAYFESISNIPKLCGTSFPELATGMVINTEWGSFDNSLDILPSTKYDELVDQNTSNKGYHLFEKRISGMFLGEILRVILIDLFERGLIFVELFRSRNGSLPHRLTEPFLLSSKVLSYLEIDDSTDLHMSELLLQNELRLPTTYSERLLIQSVTRAISRRAAYLSAIPLAAIVKRVKGQYSDEDDDFEVGCDGSLIEHYPGFRDKILEAFNTINPLEGCSKKLHIRIAKDGSGVGAALCASTVGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG13</accession>
    <name>A0A1A0HG13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_98980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713580.1">
        <property type="nucleotide sequence ID" value="XM_018859481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG13"/>
    <dbReference type="GeneID" id="30032456"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="133" mass="15315" checksum="34EB8290E60D22AD" modified="2016-10-05" version="1">MPRTWILRSRDNRTEFECLSDQAVGEDSTLYSIKLLYIHMGGLYFSCNYVFPPEAEILNDDILLGLVFLNVQFSSEISIYIYLLVPMLFLMTNRTQSFHPASQSAVRRRGGVDLRIVMVVLGSKLCWLVYIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG46</accession>
    <name>A0A1A0HG46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713309.1">
        <property type="nucleotide sequence ID" value="XM_018855397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG46"/>
    <dbReference type="GeneID" id="30028373"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="10014" checksum="40FE242AFD12D53D" modified="2016-10-05" version="1">MRFLKLRPGPGSRIHRRPSAGAAPLILWTRAILPRVQFPTLSSQQHQHQTPKHKNQKQIKSKSKTKQNKSQKQKQKQSQEQKQNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG58</accession>
    <name>A0A1A0HG58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginyltransferase</fullName>
            <ecNumber evidence="2">2.3.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_29681</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the R-transferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.8" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713630.1">
        <property type="nucleotide sequence ID" value="XM_018855457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG58"/>
    <dbReference type="STRING" id="869754.A0A1A0HG58"/>
    <dbReference type="GeneID" id="30028433"/>
    <dbReference type="OrthoDB" id="74183at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyl-tRNA--protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="ARGININE-TRNA-PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367:SF1">
        <property type="entry name" value="ARGINYL-TRNA--PROTEIN TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-end aminoacyl transferase N-terminal" evidence="6">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="N-end rule aminoacyl transferase C-terminal" evidence="7">
        <location>
            <begin position="163"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="344"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="344"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="415"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="592" mass="66245" checksum="85E7201516EC410A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGB1</accession>
    <name>A0A1A0HGB1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1769-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713398.1">
        <property type="nucleotide sequence ID" value="XM_018859345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGB1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGB1"/>
    <dbReference type="GeneID" id="30032320"/>
    <dbReference type="OrthoDB" id="2119945at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013897">
        <property type="entry name" value="Duc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF2">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
        <property type="entry name" value="Duc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="32654" checksum="228AE94F790C24D6" modified="2016-10-05" version="1">MSVKKKLLIQVGSLQHQDITVPVNTGNPVEVSTELGTFSVSVFIRNFEGSSKHKSNSLYNAFDKKYLDGTHVQDQSSTQSELPNLRILVKYIPNSNINGSNLFFGNECPVSVKDYVPATLMSTGLRLFKWFLNPTIESDLYGESPFIYGLALNSFSKLGTADVSAADFIRNENDSLEWNEQVPPLASIHERRKYFCNVDHSKKFNFEKSKSYYMMFDTNFLKLGDSSYNVSIPAFGKKTFDVNILRFANDKLDNINWTIKQSVNSSLREGIYGLVLNFRLVEESEPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGC6</accession>
    <name>A0A1A0HGC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_76058</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713389.1">
        <property type="nucleotide sequence ID" value="XM_018858470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGC6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGC6"/>
    <dbReference type="GeneID" id="30031446"/>
    <dbReference type="OrthoDB" id="333239at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005838">
        <property type="term" value="C:proteasome regulatory particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901799">
        <property type="term" value="P:negative regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF1">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="105"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="475" mass="53715" checksum="C4F5239901B6992F" modified="2016-10-05" version="1">MSFTLKIKNAGKLHEITLAEGANGSDLKTKIWEKTQIPSSRQKILIKGGKLTDEVVISSLNLNLKTHIMVLGTPDKDLPIGNAKNQVFMEELDDRQLGKTSHDPSGLQNLGNTCYLNSSLQTIFSMKDICAKIQSYNGTANPLVRALKNVFDSMSQKKENVAPMLALMQFRSQFPQFAEQEMGVYKQQDAEEAYSQLLNSLGSVLEIDDLLRITYKVLSKDLTSAEESESTEVAFKLNCYIDIKTNFLRDGIMNGLKESIEKFNDTLQVNTEHQLTKTITRLPKYLTVHFVRFFWRTDTKKKSKILRKVLFPFELDLAEMLDESIKAEKIAVRDQLRSIEKENLDLVRDYKKAKKDTSLTPQQQAEEDEMKVASIKSRFKDDFASVLPKTFDIDQATENPSSVYELTTVITHSGISADSGHYQAFSKDENDLQGDAWWRFDDNKVYPVTKEKIETLAGGGESDSALILVYKAKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGC8</accession>
    <name>A0A1A0HGC8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">DNase I-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_9511</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713700.1">
        <property type="nucleotide sequence ID" value="XM_018859402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGC8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGC8"/>
    <dbReference type="GeneID" id="30032377"/>
    <dbReference type="OrthoDB" id="387657at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070260">
        <property type="term" value="F:5'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052714">
        <property type="term" value="F:mannosyl-inositol phosphorylceramide phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072711">
        <property type="term" value="P:cellular response to hydroxyurea"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030149">
        <property type="term" value="P:sphingolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000073">
        <property type="entry name" value="Inositol phosphosphingolipid phospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051547">
        <property type="entry name" value="TDP2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822">
        <property type="entry name" value="TRAF AND TNF RECEPTOR-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15822:SF4">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="12">
        <location>
            <begin position="9"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="416" mass="46637" checksum="3AF3AA264E75F605" modified="2016-10-05" version="1">MDSKPVKLLTFNCWGLKYVSKLRRPRLRAIAEKLAHTDHDVVALQEIWVEEDWQYIEETCLAKFPHRRFFKSGIVAGPGLALLLRVPIESTFLYRFPINGRPSAFFRGDWLVGKSIAVTILGSHVPGARQLAVLNSHMHAPYKQHGDASYSTHRACQAWDLLKLVKVLQRAGYAVVQVGDLNSKPGSLPHKLFTQEGGLSDSWELLHGENTLPNDEIALLSPHEQITRGGITCDSRLNTWRAHREPWEACRLDYALIDRRALTPVAAEVLFTDLLKAPYSCSYSDHFAYSVTLHLKDQQYLEPVASKTELAKLYGETLSELQTYIDHTIPFQSNWRKLHFFASLVAVVVILVGIAFASAVQPWVSVLLALASTLIAVTGVVNGMICFLGVRSEKRALLEVKMEVEDSLRGIQRGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH23</accession>
    <name>A0A1A0HH23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9451</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713629.1">
        <property type="nucleotide sequence ID" value="XM_018859393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH23"/>
    <dbReference type="STRING" id="869754.A0A1A0HH23"/>
    <dbReference type="GeneID" id="30032368"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="7">
        <location>
            <begin position="432"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="774"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="785"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="826" mass="93301" checksum="D0DDCE3F4378269B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH65</accession>
    <name>A0A1A0HH65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_9613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713821.1">
        <property type="nucleotide sequence ID" value="XM_018859421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH65"/>
    <dbReference type="STRING" id="869754.A0A1A0HH65"/>
    <dbReference type="GeneID" id="30032396"/>
    <dbReference type="OrthoDB" id="5549748at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="39402" checksum="F0F96885C6540193" modified="2016-10-05" version="1">MSFKFPSFNLQSIQESIGKVDLESISKLIQNANPGKAVSEYSEHLKESIQPFTAKTQQLISSQLQQVQQLAASNLDANIEVSELPEDYLNLEANCDILLKLYTDLIHYTNDTYGTLSYDYPPGNSALNKIKDAHVGLMLSSKFSQLKNVSTPQEMENILLGHSAAAEAKPTADEDTAEIQVTSAQLPKTLYGQLAQIATRNGNQFAGLSDSLSFALLQISSAYIEIGSARLDQDKKVMHELNQKLVEVLNEKFIKVNELRKNMYAARLKFDLVRSQVGSEDEENEDLIAKEDDLVSATELAVLEMRKLINPLENVNLLKVFISAQKEFFDMASKRLGSLATELDKIDFQEDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHD5</accession>
    <name>A0A1A0HHD5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mitochondrial complex I NUIM TYKY subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_37293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 23 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713768.1">
        <property type="nucleotide sequence ID" value="XM_018856852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHD5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHD5"/>
    <dbReference type="GeneID" id="30029828"/>
    <dbReference type="OrthoDB" id="204405at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3270:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01351">
        <property type="entry name" value="NDH1_NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010226">
        <property type="entry name" value="NADH_quinone_OxRdtase_chainI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01971">
        <property type="entry name" value="NuoI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004538">
        <property type="entry name" value="PRK05888.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004539">
        <property type="entry name" value="PRK05888.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849:SF20">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10849">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="153"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="222" mass="25472" checksum="8E30191F035967BB" modified="2016-10-05" version="1">MLQSLARNSVRGFAFLARIAKPAATATGPVTEYLTTGGSTQAVWPKGFRQPRPQTWEESSESALLKATKFFFLSEIARGMYICMEMFFRSPYTIYYPFEKGPISPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEERIDGSRRTYKYDIDMTKCIYCGYCQESCPVDAIVESPNVEYSTETREELLYNKEKLLENGDKWEQELQYCIDADAPYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHI1</accession>
    <name>A0A1A0HHI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_109152</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714100.1">
        <property type="nucleotide sequence ID" value="XM_018853880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHI1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHI1"/>
    <dbReference type="GeneID" id="30026856"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015916">
        <property type="term" value="P:fatty-acyl-CoA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042758">
        <property type="term" value="P:long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF67">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY D MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="533"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="757"/>
            <end position="794"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="810" mass="93542" checksum="E2009CDF0757CB34" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHJ4</accession>
    <name>A0A1A0HHJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713956.1">
        <property type="nucleotide sequence ID" value="XM_018856798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHJ4"/>
    <dbReference type="GeneID" id="30029774"/>
    <dbReference type="OrthoDB" id="4079976at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="312" mass="34047" checksum="887638FFB394937E" modified="2016-10-05" version="1">MTYLIGDRRISLSGPPLSAEEQRVVRAHPRLFQACLNASAHRYLLSSKQIVHLYTDIVTIYSTLLLKIAHYDDVRAMAAKTRAALARLETDLGSVLGRPVHGPGVGWELLPYEAVVKKGALVVLQACGAAFAAVHRTLCCIDVFFGAFDNLKKLLVPCLATHTRMVQKLLGGAAGLLRLQTIVDQIGMLPELTPSVAKQLQKIAAITGGLEREARLLDMVFAAVNPSLLLYTAVLLGRAILKRDVAARSCQIVARRDVCYQAAEHVVSIPLSGSPAPSLYMYESTRHVLYFFSAVLVVVLAVLFRLYNVVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHK8</accession>
    <name>A0A1A0HHK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">HMG-box</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_29942</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713971.1">
        <property type="nucleotide sequence ID" value="XM_018855518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHK8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHK8"/>
    <dbReference type="GeneID" id="30028494"/>
    <dbReference type="OrthoDB" id="10070927at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22016">
        <property type="entry name" value="HMG-box_NHP10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056513">
        <property type="entry name" value="INO80F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09011">
        <property type="entry name" value="HMG_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24245">
        <property type="entry name" value="INO80F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="98"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="98"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="187"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="271" mass="30350" checksum="25305F3E713E5DB1" modified="2016-10-05" version="1">MEESNEVATVALLRTRNSIRRLRLEYAVLLERLETQVVRILEDGNPKLSEAMARPFSPVLTDDNLEAKLAKVKVARPKKAPGVSRAALKRAARDPNLPKRPTNAYLMFCDREKDRVRSELERQNPGQPATELTRALTDAWKLLSDEQKIPYLELYEIDRERYQREMMVYNEQKHDETLVKGKLVAQASPGLGPVEPQGSTEPGTPTQNAEEVENNDENGAEDEGLEDEPEAQDELDDDATLGLKREFGSSVESGSLPEQGAEGGAEKRQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHN8</accession>
    <name>A0A1A0HHN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alpha-1,6-mannosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_107145</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714002.1">
        <property type="nucleotide sequence ID" value="XM_018853829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHN8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHN8"/>
    <dbReference type="GeneID" id="30026805"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000136">
        <property type="term" value="C:mannan polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000009">
        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000002">
        <property type="entry name" value="Initiation-specific alpha-1,6-mannosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039367">
        <property type="entry name" value="Och1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834:SF11">
        <property type="entry name" value="GLYCOSYLTRANSFERASE HOC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31834">
        <property type="entry name" value="INITIATION-SPECIFIC ALPHA-1,6-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="394" mass="45464" checksum="7177BD1F9386BF19" modified="2016-10-05" version="1">MKTNRMTNRRKYIGITIVLFVALAGLLRWSTARSKDTLAADNFNGLYLLVFSENDKKQDEFLRRIEKMHAKFYALQEKRLAHLEQQNDRLLAQIETLKVPPATMSVREKLVYMVPYVARSRFPAYIWQTWKHGLNDDRFDANFKEGQAQWAFRNPGFVHELFNDDTSLATIKHLYSHVPEVLDAYESMPEVVLRMDFFRYLILFARGGVYADIDTMPLQPVPNWIPENVEATELGMIICVGNEATSKNWRAEAHRRLEFGQFIIQSKPGHPVLREIIAQITEATLAKKRALRDGVLMRTSDSPNQKLLDISRWTGSGVWTDVVFEYLNDYVRAAIYQLVSWQDFHALQGPKLVSDILILPTKSFASELEVPKDGKIADPIAFAKHWSANIWKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHS8</accession>
    <name>A0A1A0HHS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_35837</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714195.1">
        <property type="nucleotide sequence ID" value="XM_018856658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHS8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHS8"/>
    <dbReference type="GeneID" id="30029634"/>
    <dbReference type="OrthoDB" id="7396853at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.830.10">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30096:SF0">
        <property type="entry name" value="4,5-DOPA DIOXYGENASE EXTRADIOL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53213">
        <property type="entry name" value="LigB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Extradiol ring-cleavage dioxygenase class III enzyme subunit B" evidence="6">
        <location>
            <begin position="33"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="329" mass="36672" checksum="8DCF8DD632854CC0" modified="2016-10-05" version="1">MSAYKSTLGKSVPAAAQSAAFRNAEPNTNPYPALFVSHGGPTFMYEDDDFGNKGAWRAVRKLGSTIKNDWRPDYILVVSAHWQLGGLNLIEVAVPPKNPLIYDFYGFPDHMYKEQFRTWNSQVVARLVQENLEQKGFHARLTERGLDHGVWVPLKVAFPQGPGDDDMALPHTPLIQVSLTGREGDFETHARLGEVLSHFQNNLIWDPVQNRHLKGLVICSGMSVHNLRDLGRAVHSGQRALPYVGEFSRLVSQALQQGPDTLPLLEKLKTEHSSLLRAAHPTLEHFVPLVVAAGINRASGNRKVTELYSDNQLSLAWGTYQFGEYSAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI98</accession>
    <name>A0A1A0HI98_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">N-terminal nucleophile aminohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9967</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714211.1">
        <property type="nucleotide sequence ID" value="XM_018859500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI98"/>
    <dbReference type="STRING" id="869754.A0A1A0HI98"/>
    <dbReference type="GeneID" id="30032475"/>
    <dbReference type="OrthoDB" id="14446at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052373">
        <property type="entry name" value="Gamma-glu_amide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="40173" checksum="5423D117B0561D99" modified="2016-10-05" version="1">MCRFMIYKGKRRMMLSDLLTRPAHSIINQLFDSRLRLDMRNPINGDGFGIGYYSEKKDACVFRAITPAWNNVNLNSLSNCTESGLVFAHVRASTQGVLAETNCHPFQYGRILFMHNGGILAFDKIKRKLVMHLDDRFFLFIQGSTDSECCCALFLDTLYKMGYDPDDAKVEFSHKILREALLSMIELIKNWQVELTSEPSLMNFAVTDGDSVVVSRYITSKTDEAASLHFSTGSRFFEYEPGLFKMERLNRSQDVIFVASEPLTFERGDWTCVPSNTVISITKNNTVLMHPIMDEFYNSKISDRSSGFAMSKGLIGGVLQKNAEKEESGNAMETLVSHLPPLEREGRRVAEKLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIE0</accession>
    <name>A0A1A0HIE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_76724</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714247.1">
        <property type="nucleotide sequence ID" value="XM_018858575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIE0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIE0"/>
    <dbReference type="GeneID" id="30031551"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="18"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="161"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="23"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="331" mass="34626" checksum="B8800B875F920308" modified="2016-10-05" version="1">MFKSASRSFSSSASAAYKVAVLGAGGGIGQPLSLLMKLNHKVTDLALYDLRGAPGVAADVSHVPTNSTVKGYEPEGLKDALTGADVIVIPAGVPRKPGMTRDDLFNTNASIVRDLAKAAAEYAPNAALCIISNPVNSTVPIVAEVFKSKGVYNPKKLFGVTTLDVLRASRFVSEIAGTNPVDEHVTVVGGHSGITIVPLLSQTNHKDLDVETRDALIHRIQFGGDEVVQAKNGAGSATLSMAQAGARFAGAVLNGLAGEADVIEPSFVESPLFKDEGVDFFSSKVTLGVEGVKTIHALGELSAHEEELVKKAKETLITNIKKGVDFVKQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HII7</accession>
    <name>A0A1A0HII7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycogen synthase kinase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_76674</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Protein kinase that acts downstream of the MPS1 MAPK cascade as a highly conservative signal modulator that dictates growth, conidiation and pathogenicity. Phosphorylates HAT1 at 'Ser-8' to block its translocation from the nucleus to the cytoplasm where HAT1 positively regulates appressorium development and pathogenicity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714178.1">
        <property type="nucleotide sequence ID" value="XM_018858569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HII7"/>
    <dbReference type="STRING" id="869754.A0A1A0HII7"/>
    <dbReference type="GeneID" id="30031545"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000009">
        <property type="entry name" value="Glycogen synthase kinase-3 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000082">
        <property type="entry name" value="Shaggy-related protein kinase kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0309">Germination</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="408" mass="46992" checksum="EA8FB3689CD03F4F" modified="2016-10-05" version="1">MSPIEIVTESVNNGRTGELESIQYTQSQVVGHGSFGVVSQTQILPSNEIAAIKRVLQDKRFKNRELQIMKLVHHRNVVDLKYYFYTNNDKDELYLNLILEFVPETLYKASHYYVSKRLSMPALEVKLYTYQMFRALNYIHTQGICHRDIKPQNLLINAETGVLKLCDFGSAKILNPAEPNVSYICSRYYRAPELIFGATNYTTKIDVWSAACVMAEMIMGQPLFPGDSGVDQLVEIIKILGTPTREQIKCMNPNYTEYKFPQIKPIPLSKIFKKMPTDCINFLAQILEYSPNERISCAQALADPYFNELRKEGTMLPNYRKLFSQQFYSTSSHNPYQSNANYQLYSNQPDSRELPELFDFDDRELSMEPSLNHLLVPDFAHKRLKITRGSSLEDFAPMSEAEMKVTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIT8</accession>
    <name>A0A1A0HIT8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_36451</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714234.1">
        <property type="nucleotide sequence ID" value="XM_018856740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIT8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIT8"/>
    <dbReference type="GeneID" id="30029716"/>
    <dbReference type="OrthoDB" id="25131at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039328">
        <property type="entry name" value="WDR89"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22889:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 89"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="383" mass="42071" checksum="8E71B38574B0E36A" modified="2016-10-05" version="1">MTAVLKQSWLPFLDSWALALQHFPLISNGSFVTSSSSGALALCALDGPTQPLQINKTAHEACVNAIDKIDQYTLASASADGIKVWDLRQSLNRPQLSLTNSRKLTFLSLGSNAGHWLAGGTELVGTDAELHLWDLKSPAHAVRLFVDSHHDDITAVTFHGSLPYLMSGSTDGYVNVYNLGEPDEDEALHQVINFASVHLCHFVQPNRISVLSHMETLAFWELNNTNYEAAEEPRPRVLGDVRAVWPDCEYVVDIHDAGYVSYGANLKLSLTLVPFDAKSEGFDASGSISLLPAHGEDVVRDVMFIPGTRGVLTCGEDGLVKAWELPSEVPSGLHAVGDALADNGVSTQSSDKDRKRQERKERKKKKDRKDKNKPKKDARFKPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIT9</accession>
    <name>A0A1A0HIT9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_117096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714555.1">
        <property type="nucleotide sequence ID" value="XM_018854070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIT9"/>
    <dbReference type="GeneID" id="30027046"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="140" mass="14663" checksum="24C2BF2EE9C5B6DF" modified="2016-10-05" version="1">MAGIGSFISQGNSSLIGGALRRPLALLGIRLAGWGPVVAGPASGWGGGPSLRTPGGARVTVSTLRGQMRGSDWSEVCSGGAPPGRRTTRADFRLPRGRPWLAEKLTGSRTRPLVQTIYTRQFPAAPHHNCASSQDIFICA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ70</accession>
    <name>A0A1A0HJ70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_116728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714527.1">
        <property type="nucleotide sequence ID" value="XM_018854056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ70"/>
    <dbReference type="GeneID" id="30027032"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="128" mass="15111" checksum="09EFD83739DF17FE" modified="2016-10-05" version="1">MIENVMIYSMCMPKAIEMEITNAKSEASKDIITELENQKSSEFFLVKNNINLIKKYFPNIQEKRDRFRNENRTIASAMLKNLPFLVKENEVSRQKMGDVGHVIATKVDSTKIWKLEKFYRLEKFGRAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJB2</accession>
    <name>A0A1A0HJB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_35900</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M67A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714409.1">
        <property type="nucleotide sequence ID" value="XM_018856668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJB2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJB2"/>
    <dbReference type="GeneID" id="30029644"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08062">
        <property type="entry name" value="MPN_RPN7_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000004">
        <property type="entry name" value="26S proteasome regulatory subunit rpn-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033858">
        <property type="entry name" value="MPN_RPN7_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="5">
        <location>
            <begin position="15"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00072102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="320" mass="36225" checksum="0BE37B3EEA7EAACD" modified="2016-10-05" version="1">MSSSIKENTFLSKTVTVSPLVLLSVADHYDRVAKDSKKRVVGVLLGDSESNTIKVTNSFAIPFEEDEKNSSVWFLDHNFIESMTEMFKKINAKEKLIGWYHSGPKLRASDLKINDVFKKFTPSPLLVIVDVQPRTVGIPTDAYFAVDDIKHDGSKAERTFVHIPSLIEAEEAEEIGVEHLLRDIRDQAAGNLSLRVTERYQSLMGLHQKLREIANYLRKVNEKELPLNHLILGKLQNVFNLLPNLAQSTGDSDKIFESIASERAENSSSPLATAFTVKTNDEMMMIYTSTLVRSIIAFHDLIENKLENKKLIEKRKEIHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJB5</accession>
    <name>A0A1A0HJB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_35830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714730.1">
        <property type="nucleotide sequence ID" value="XM_018856657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJB5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJB5"/>
    <dbReference type="GeneID" id="30029633"/>
    <dbReference type="OrthoDB" id="5423111at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032126">
        <property type="term" value="C:eisosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052649">
        <property type="entry name" value="NCE102-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28165:SF1">
        <property type="entry name" value="NON-CLASSICAL EXPORT PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="6">
        <location>
            <begin position="6"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="160" mass="17118" checksum="CF497FB4DC998808" modified="2016-10-05" version="1">MLAIGDVILRGINFVFLVIALGLTGSLAATTVYQLNSQVNFAVFAAAFGLLTSSFYGVLAYFVASFAWPLILAVLDFLNLVFTFAAATAIAANIRAHSCTNQDYLDSNTITQGETDRCRKAQASVAFIYLSFAMFLASGVFSMISLFKGGMFGSKNTSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJK7</accession>
    <name>A0A1A0HJK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1746 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_37624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714830.1">
        <property type="nucleotide sequence ID" value="XM_018856899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJK7"/>
    <dbReference type="GeneID" id="30029875"/>
    <dbReference type="OrthoDB" id="4094266at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038967">
        <property type="entry name" value="Dsc4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013715">
        <property type="entry name" value="DUF1746"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39405">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39405:SF1">
        <property type="entry name" value="DSC E3 UBIQUITIN LIGASE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08508">
        <property type="entry name" value="DUF1746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1746" evidence="2">
        <location>
            <begin position="41"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="243" mass="27811" checksum="B32255455D394981" modified="2016-10-05" version="1">MPDLFTSLRYPSVEETLRNNASLLIKQKRLFLADLRTLMGTLGCALIGILYLRDMSILLFCLRATVQFLLSSPYAAHFNYGTLLQEEARKEQRWFLFWSVVLVNGVSFLDHLILGTYRTSSAGDRQLHGSWTVQFIGERLPRGRSELVVFDGLIVVMQLVYFCLMCTTSDLAVLQGPLADLSLKDEAVLLDVVSDGFDGNVHILTVDVWEGVRALWRYDRHPENRDVQELRSMREQALRRILV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJW8</accession>
    <name>A0A1A0HJW8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="21">ATP-dependent metallopeptidase Hfl</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_30608</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the 850 kDa m-AAA protease complex, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. Associates with the prohibitin complex, composed of PHB1 and PHB2, inhibiting the activity of the m-AAA protease complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714777.1">
        <property type="nucleotide sequence ID" value="XM_018855668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJW8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJW8"/>
    <dbReference type="GeneID" id="30028644"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="20">
        <location>
            <begin position="333"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="47"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="56"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA24296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="788" mass="87374" checksum="4E307F4411676723" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKL6</accession>
    <name>A0A1A0HKL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Gluconokinase</fullName>
            <ecNumber evidence="3 9">2.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30803</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>D-gluconate + ATP = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate acid metabolism; D-gluconate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="3 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715051.1">
        <property type="nucleotide sequence ID" value="XM_018855715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKL6"/>
    <dbReference type="GeneID" id="30028691"/>
    <dbReference type="OrthoDB" id="275177at2759"/>
    <dbReference type="UniPathway" id="UPA00792"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01313">
        <property type="entry name" value="therm_gnt_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442:SF3">
        <property type="entry name" value="GLUCONOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="179" mass="19544" checksum="2E43B053C7E7EEC8" modified="2016-10-05" version="1">MPTVIIVGGPSGTGKTTTAALLASHFDCPYIEGDELHPEENVAKMARGEPLTDDDRWGWLKKISRASVGKAADPANKSGICVASCSMLKRAYRDYLLANGNGADFRFVFLHASFESIIKRVAARQNHFLKLNMVKSQFDIMDVPKSEELIRNGGKAIAVDCETGTPQQLAELIVHQLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKM4</accession>
    <name>A0A1A0HKM4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">AICARFT/IMPCHase bienzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_133561</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Also catalyzes the cyclization of FAICAR to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714923.1">
        <property type="nucleotide sequence ID" value="XM_018854344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKM4"/>
    <dbReference type="STRING" id="869754.A0A1A0HKM4"/>
    <dbReference type="GeneID" id="30027320"/>
    <dbReference type="OrthoDB" id="6017153at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.440:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005492">
        <property type="entry name" value="PRK07106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000414">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="589" mass="64443" checksum="72DA5CD47FD94A1C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKX0</accession>
    <name>A0A1A0HKX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_10678</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715023.1">
        <property type="nucleotide sequence ID" value="XM_018853824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKX0"/>
    <dbReference type="STRING" id="869754.A0A1A0HKX0"/>
    <dbReference type="GeneID" id="30026800"/>
    <dbReference type="OrthoDB" id="1915122at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="7">
        <location>
            <begin position="88"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="212" mass="23929" checksum="2518357BD7713E25" modified="2016-10-05" version="1">MKILNDEERDSHWQTVLIEGLKGTVVGAGLAGLMVAGVKRRYPVPYKNFNASIKAAMWAMPTISMAAFYADDGSVKFDEQTHRSDYLEKVEKERLERWETLSTGDKIFTKVNDNKYKIIVGAWAASLWGSWAIVNKDKYMTTAQKAVQARVYAQAITVVLLLGTLLLSMHEKKLADSLPAPVPEWKRYLDEQESEKQAIQQHAKAQAEVPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1N7</accession>
    <name>A0A1A0H1N7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13862</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709339.1">
        <property type="nucleotide sequence ID" value="XM_018854438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1N7"/>
    <dbReference type="GeneID" id="30027414"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="112" mass="11891" checksum="C7BB7BEB1C0775B5" modified="2016-10-05" version="1">MEEAGDNDDASEAPDDSGYIEEAGDNDDASEEPEAPDETGLAEDKDEADVIASDAAECEKDSDDVAELVEDSDDTCESVEETDDKCGTDDRAGDVCEAELIDDAFEEIDDKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1V3</accession>
    <name>A0A1A0H1V3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_13920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709404.1">
        <property type="nucleotide sequence ID" value="XM_018854451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1V3"/>
    <dbReference type="GeneID" id="30027427"/>
    <dbReference type="OrthoDB" id="4094547at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="39903" checksum="56229091071B3C0B" modified="2016-10-05" version="1">MRHSTLLFAQHTYKSLQLLLPQPYKLFHKSLLNLSQNTTSTPATSWNESLKGFDLFLKVSGLKAAHLQKLWDRLAGDQKEVFLNPPPEVHEQWVRIAQGYKEYEKVQVERETANPFYHIPLYPWEIIKDKLRHKPMFSSDVYDQLIQEAMAPSMKLLQPAKIYENFCINFEPENPHQQFEDLPSSQITRYKIEAERKLAYAKRIMAYRKAKKALRHSNTFINDYTRELGESLTGQEKKDFIQQQWEFEQLLDMLSNQRTVFKEINLEIHLSMVMDYIFEYNEVYDCGVGWRQWRQHACGNYHYLDRILFPVVVNATEEKIQLLDSLPSPPGWLGPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4J4</accession>
    <name>A0A1A0H4J4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">SWI5-dependent HO expression protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SHE3</name>
        <name evidence="12" type="ORF">METBIDRAFT_28760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the SHE3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709496.1">
        <property type="nucleotide sequence ID" value="XM_018855359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4J4"/>
    <dbReference type="STRING" id="869754.A0A1A0H4J4"/>
    <dbReference type="GeneID" id="30028335"/>
    <dbReference type="OrthoDB" id="6088208at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031398">
        <property type="entry name" value="She3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17078">
        <property type="entry name" value="SHE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="15"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA18961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="274" mass="31621" checksum="B91795839B61C0D6" modified="2016-10-05" version="1" fragment="single">MEDSPRKQQSTSRVIDSLHSQIDDLKHELEVLRLSCGDYKKKSTILASKNDSLVDQLANCKHENDMVNALLKRKERRIVDLESEFDDLSLESESLKQSVKNYKIRCENLQESSASSTAEHERLKISYDALMASQTEYKRHYQDELNKLTNQLETYRLDSIRDMRDLMEKLEGNDKDVDALLEGLNLKRTSMDNMLVDRNKSILGLLSLLAKVAKTHGEECKSILLDNVDIINVLRHKHPDIFDMVTERSETLIDLDDLIRESAVSLETSFYDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V1</accession>
    <name>A0A1A0H4V1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_230642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709488.1">
        <property type="nucleotide sequence ID" value="XM_018855254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V1"/>
    <dbReference type="GeneID" id="30028230"/>
    <dbReference type="OrthoDB" id="10688527at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="848"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="854"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1103" mass="124649" checksum="3079333FC93D2319" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V3</accession>
    <name>A0A1A0H4V3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13816</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709601.1">
        <property type="nucleotide sequence ID" value="XM_018854431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4V3"/>
    <dbReference type="GeneID" id="30027407"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="101" mass="11485" checksum="739C2E5D5E41FD29" modified="2016-10-05" version="1">MEGLADKVASSTVNVGSHRTFTKFPDLNADDDLGEEIHFMETTKAETRESQIMVPGVVRTSTTKRQLDDIESIFSDKPRKKKKEKKQKEKKKKNAIDEIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H545</accession>
    <name>A0A1A0H545_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_220095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709731.1">
        <property type="nucleotide sequence ID" value="XM_018855130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H545"/>
    <dbReference type="GeneID" id="30028106"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="205" mass="22694" checksum="034CC3A107902649" modified="2016-10-05" version="1">MGHTDMASICMNCIGPNIGPRRTCILSSGRAGTQIGKKQGKGTFCFVFGGSRRKSVFWKDVRKCVVCFCRKGRSIIPGPKDLPAASLYIGRCEIFAVAPNYAFIVRCELLATQAAVLLTDHVWGPSPVKSKRRPEEKVQGFWPRLRQRVKKRNEKKFAYFQKMSLLPCQVADQDPKTGLGVQAQSGNGLLVFRLTGIASFFESSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5E1</accession>
    <name>A0A1A0H5E1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">E set domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_47055</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rho GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709670.1">
        <property type="nucleotide sequence ID" value="XM_018858118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5E1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5E1"/>
    <dbReference type="GeneID" id="30031094"/>
    <dbReference type="OrthoDB" id="1683373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005094">
        <property type="term" value="F:Rho GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.30">
        <property type="entry name" value="Coagulation Factor XIII, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000406">
        <property type="entry name" value="Rho_GDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024792">
        <property type="entry name" value="RhoGDI_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980:SF3">
        <property type="entry name" value="LD16419P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10980">
        <property type="entry name" value="RHO GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02115">
        <property type="entry name" value="Rho_GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="134" mass="15414" checksum="08A6027135EF59B9" modified="2016-10-05" version="1">MSVSHPNLEILGFTVNYEDGTKNEIPVKGSKSIEFKIPEDTTYQTTIHFLVKEKTLNKLKYKQEVKAFGMVVRSREVFVGEQFAPRDTPYDVTFDKDCTPKGMILRGEYNCVSTYYADGEVLFQSPWKLNVTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5H7</accession>
    <name>A0A1A0H5H7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent 6-phosphofructokinase</fullName>
            <shortName evidence="14">ATP-PFK</shortName>
            <shortName evidence="14">Phosphofructokinase</shortName>
            <ecNumber evidence="14">2.7.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphohexokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_46425</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14 15">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">Allosterically activated by ADP, AMP, or fructose 2,6-bisphosphate, and allosterically inhibited by ATP or citrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14 15">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade "E" sub-subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.11" evidence="14"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709876.1">
        <property type="nucleotide sequence ID" value="XM_018858038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5H7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5H7"/>
    <dbReference type="GeneID" id="30031014"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000007">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.460:FF:000008">
        <property type="entry name" value="ATP-dependent 6-phosphofructokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03184">
        <property type="entry name" value="Phosphofructokinase_I_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009161">
        <property type="entry name" value="6-Pfructokinase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040712">
        <property type="entry name" value="Pfk_N"/>
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        <property type="entry name" value="Phosphofructokinase_CS"/>
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        <property type="entry name" value="Phosphofructokinase_dom"/>
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        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02478">
        <property type="entry name" value="6PF1K_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF57">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PFK"/>
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        <property type="entry name" value="PHFRCTKINASE"/>
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        <property type="entry name" value="Phosphofructokinase"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="10 14 15" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="6 14 15" id="KW-0808">Transferase</keyword>
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            <begin position="4"/>
            <end position="103"/>
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            <begin position="208"/>
            <end position="515"/>
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    <feature type="domain" description="Phosphofructokinase" evidence="16">
        <location>
            <begin position="596"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal catalytic PFK domain 1" evidence="14">
        <location>
            <begin position="1"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interdomain linker" evidence="14">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal regulatory PFK domain 2" evidence="14">
        <location>
            <begin position="596"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="279"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="309"/>
            <end position="312"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <begin position="355"/>
            <end position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="392"/>
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        <ligand>
            <name>substrate</name>
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        </ligand>
    </feature>
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        <location>
            <begin position="399"/>
            <end position="401"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="456"/>
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        <ligand>
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        <location>
            <position position="483"/>
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        <ligand>
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        </ligand>
    </feature>
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        <location>
            <begin position="489"/>
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        <ligand>
            <name>substrate</name>
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        <location>
            <position position="666"/>
        </location>
        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
            <note>allosteric activator; ligand shared between dimeric partners</note>
        </ligand>
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        <location>
            <begin position="723"/>
            <end position="727"/>
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            <name>beta-D-fructose 2,6-bisphosphate</name>
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            <position position="761"/>
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        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
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            <begin position="768"/>
            <end position="770"/>
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            <name>beta-D-fructose 2,6-bisphosphate</name>
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        <location>
            <position position="828"/>
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        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58579"/>
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            <position position="854"/>
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        <ligand>
            <name>beta-D-fructose 2,6-bisphosphate</name>
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            <begin position="860"/>
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            <name>beta-D-fructose 2,6-bisphosphate</name>
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            <name>beta-D-fructose 2,6-bisphosphate</name>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03184"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000533"/>
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    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
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    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF18468"/>
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        <source>
            <dbReference type="EMBL" id="OBA19344.1"/>
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    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="979" mass="107222" checksum="3D55C0A70F3809F0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5J1</accession>
    <name>A0A1A0H5J1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor subunit alpha homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Docking protein alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_46804</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="OBA19359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018858081.1"/>
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    <dbReference type="OrthoDB" id="1727884at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
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        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="10">
        <location>
            <begin position="516"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="543" mass="60502" checksum="71E6421035092ADA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5J5</accession>
    <name>A0A1A0H5J5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709896.1">
        <property type="nucleotide sequence ID" value="XM_018854403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5J5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5J5"/>
    <dbReference type="GeneID" id="30027379"/>
    <dbReference type="OrthoDB" id="8249012at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21521">
        <property type="entry name" value="AMUN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21521:SF0">
        <property type="entry name" value="AMUN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="32997" checksum="22B0CD4470475EB7" modified="2016-10-05" version="1">MSTGILGILRNAAAELYPLITKELSKQSTKKYNRDEWTLCELDTWKNTSFPRLLKERHDSEKSLYMTKDELVLLMDWKLAKGKFRPSLPKLIKSNSPESVLNFSREGYSIFMSYIELTGANDWTHISTEDYKITIRASLKALCQLKGVGPATASLMLSLLCEVTKFAPPFFSDESFMYFIRDPLRPLQPIKYTVREYVEEFVPVLAKMSAQDRLSSPAILEKGSWSLKMYHLYRIDRLTDLELPFEISDESAEAFKDISKYISGNIPEKRTTTREGISGGKKRKTLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5J6</accession>
    <name>A0A1A0H5J6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminopeptidase P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33382</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709704.1">
        <property type="nucleotide sequence ID" value="XM_018856278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5J6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5J6"/>
    <dbReference type="GeneID" id="30029254"/>
    <dbReference type="OrthoDB" id="4215474at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF4">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="7">
        <location>
            <begin position="50"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="514" mass="57051" checksum="A5425BFA14B5FD06" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5M8</accession>
    <name>A0A1A0H5M8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lethal giant larvae (Lgl)-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709787.1">
        <property type="nucleotide sequence ID" value="XM_018856302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5M8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5M8"/>
    <dbReference type="GeneID" id="30029278"/>
    <dbReference type="OrthoDB" id="19944at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF25">
        <property type="entry name" value="TOMOSYN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lethal giant larvae (Lgl)-like C-terminal" evidence="1">
        <location>
            <begin position="548"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1063" mass="115750" checksum="ABFA59FAA893E85B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5N1</accession>
    <name>A0A1A0H5N1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 12">Adenylosuccinate lyase</fullName>
            <shortName evidence="12">ASL</shortName>
            <ecNumber evidence="6 12">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_46842</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = fumarate + AMP</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="6 12"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709755.1">
        <property type="nucleotide sequence ID" value="XM_018858087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5N1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5N1"/>
    <dbReference type="GeneID" id="30031063"/>
    <dbReference type="OrthoDB" id="406045at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03302">
        <property type="entry name" value="Adenylsuccinate_lyase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.60:FF:000001">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000005">
        <property type="entry name" value="Adenylosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43172:SF1">
        <property type="entry name" value="ADENYLOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Adenylosuccinate lyase C-terminal" evidence="13">
        <location>
            <begin position="373"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="481" mass="53654" checksum="D761D394DB27500C" modified="2016-10-05" version="1">MSNDSYSTPLSSRYASEEMSLIFSLRNRFSTWRKLWLNLAIAEKKVGLSVITDEAIAEMEKHLTITDEEIAAASVEEAKVRHDVMAHVHVFGETCPAAAGIIHLGATSCFVTDNADLIFLRDAYDVLIPKLVNVIDRLSKFALQYKDLPVLGWTHFQPAQLTTVGKRATLWIQELLWDLRNMVRARNDLGLRGVKGTTGTQGSFLSLFHGDHDKVEELDQTVVNLLGFEHVYPVTGQTYSRKIDIDVLSPLASLGATTHKFATDVRLLANLKEVEEPFEKSQIGSSAMAYKRNPMRCERVCSLSRHLGGLLNDAIQTASVQWFERTLDDSAIRRISLPSAFLTADILLSTMINITSGLVVYPKVIERRISAELPFMATENIIMAMVEKGGSRQDCHEEIRVLSHQASAVVKQEGGDNDLIERVRNTEYFKPIWAELDSLLDPSTFVGRAPQQTEKFVAKDVAEALAPFKDMITTETVSLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Q7</accession>
    <name>A0A1A0H5Q7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Vacuolar protein-sorting-associated protein 46</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709780.1">
        <property type="nucleotide sequence ID" value="XM_018858049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Q7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Q7"/>
    <dbReference type="GeneID" id="30031025"/>
    <dbReference type="OrthoDB" id="10266568at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904902">
        <property type="term" value="P:ESCRT III complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10476">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="22283" checksum="33C228EBF821395A" modified="2016-10-05" version="1">MAGLEQSLFQLKFTAKLLNRQASKAAKEELQEKTKTKKALMQGNKDIAQLYAQNAIRKSNERVNLLRLASRIDAVASRVQTAVSMRSVTGNMTTVIKGMDKALQTMNLERISVVMDKFENQFEELDALTNYYESATNNVNALTTPQEDVDELMSQIADEAGIEMKQGLNETKVDIVSPPSAVSEEKEDRLAERLRALRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5W4</accession>
    <name>A0A1A0H5W4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Serine hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_46884</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709829.1">
        <property type="nucleotide sequence ID" value="XM_018858097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5W4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5W4"/>
    <dbReference type="GeneID" id="30031073"/>
    <dbReference type="OrthoDB" id="2094269at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF6">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="299" mass="33134" checksum="9C1097BD5580B184" modified="2016-10-05" version="1">MSGKKVVEFKGRVLFLHGFTQSSSVFYAKTSALRKKLKALKYQAVYLNAPLKLTPSQLPSSDSLSKFNTVAAEPDEETNYRAWWFRDNNGKVTLDHAINAVRQYIEHGTIIDGSPEDQKIKADLFSENDDIGSSGEANLENFESSNKSELPPIVGIIGFSQGACLAGGLVDQFEKMFGVPLKFAVLYSGFKLDTTLMPEYKSLYTSDDGQSTNANLLHVIGELDTIVGDDRAYTLYDISKKNSHVLKHPGGHFVPNSKLLVNQVTNWIQSVDVEAERKEKQETQSIDDDLLAAFDKLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H630</accession>
    <name>A0A1A0H630_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_222154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709905.1">
        <property type="nucleotide sequence ID" value="XM_018855150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H630"/>
    <dbReference type="GeneID" id="30028126"/>
    <dbReference type="OrthoDB" id="4026481at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="104" mass="11861" checksum="68ACFB35060F31D1" modified="2016-10-05" version="1">MSFYQDHKRPADFCLTPPLTPQRSHKRGRDDMEQLSPDTKRIQFQQIHSQAHLSTVKMMMDASRKQSLGAHTQTPDAGDDLAGAEPSYTPQKPFWPAIARLRQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H647</accession>
    <name>A0A1A0H647_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709958.1">
        <property type="nucleotide sequence ID" value="XM_018857978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H647"/>
    <dbReference type="GeneID" id="30030954"/>
    <dbReference type="OrthoDB" id="4089586at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="331" mass="36122" checksum="75AA1BB13DED6B23" modified="2016-10-05" version="1">MSVLADERLQLALQLKEIEHRDENRKHLIRLCVMKALCLTQEATVPASSQDPVNFDALQKKVHAFELQVASLKMQLSTKEQAHAAKVDDLREEIARLKKNSDFCAGSNVARASTRASRGRVKLAAVGSPVSRLTISMSLFFAKSPFSDLKSGRGNSFSGDGIASVASQASFKLSRSGPKPIEEVFSPNNSSAESTPKKSKTNSDILQESSRIEPQGLSGIGTEKRASSETLTTSNTTVSDPEETFLSANMTFESEKEKKTKRKKIRLVSSEASKLILAQHDGGHDEEKDLNSLKYYQDENFESGSGSPARPQKRPAAAGPVTKKKHVFKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L8</accession>
    <name>A0A1A0H6L8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_23020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710133.1">
        <property type="nucleotide sequence ID" value="XM_018855242.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6L8"/>
    <dbReference type="GeneID" id="30028218"/>
    <dbReference type="OrthoDB" id="18530at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40368">
        <property type="entry name" value="YALI0F14399P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40368:SF1">
        <property type="entry name" value="YALI0F14399P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="267" mass="29551" checksum="4E14D2424B6DF65B" modified="2016-10-05" version="1" fragment="single">LSAASGKAQFFPLTRKGEIPTIQPYALGQEVLVDCISRNIDNGEHKFDSNDRIIYKAFPTCKETGKPLTFKYGVSEDVNCTISFSDELYHLFQLYVHEDAPFSCRIPISSEANYLELGGAHIPLTFNLRGEVHDSHLDIDSQMNVVMTRPALKRPEQQTVVNAAAWSAGTNATRLTIGESLTLELAVRWFDSIKPAGSQGTADLPFTDGFYKLPMYSVPVSYMQYAMSLVVVALVSAVVSGFISFRLKKTRGYHPLSDREAGVAKQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S0</accession>
    <name>A0A1A0H6S0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein S17e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_13355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710135.1">
        <property type="nucleotide sequence ID" value="XM_018854343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S0"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S0"/>
    <dbReference type="GeneID" id="30027319"/>
    <dbReference type="OrthoDB" id="1727351at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_eS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="137" mass="15811" checksum="D8A297609848B7D5" modified="2016-10-05" version="1">MGRVRTKTVKRASKVLIERFYPKLTLDFETNKRLTTEIAEIQSKRLRNKIAGYTTHLMKRIQKGPVRGISFKLQEEERERKDQYVPEVSALDLSHTNGQLEVDAETADLVKTLGFKIPLQTVAISSQRGPRRFAKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S2</accession>
    <name>A0A1A0H6S2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit alpha type</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_84430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710183.1">
        <property type="nucleotide sequence ID" value="XM_018859238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S2"/>
    <dbReference type="GeneID" id="30032213"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03751">
        <property type="entry name" value="proteasome_alpha_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000044">
        <property type="entry name" value="Probable proteasome subunit alpha type-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="6">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="297" mass="32711" checksum="F81EA8B85E3FCFF3" modified="2016-10-05" version="1">MKNLAACGTNASRDQSHRSGDLVAVCPRALHQPYTSTMTSIGTGYDLSNSVFSPDGRNFQVEYAMKAVENGGTSIGIKAKDGIVLAVEKIVNSKLLVPGRNKRIQTIDRHIGVAYSGLLPDGRHFVNRGRDEAQSYSSIYKTPVGVPALMDRLGLYVQNYTCYNSVRPFGIVSIVGGVDENGPHLYMVEPSGAFWGYSGAASGKGRQTAKPELEKLDFELLTVREAVKHAARIIHQAHEDNKDKDYELEISWCSLEETGGQHQFVPEDLLQEAIKLAQEEDDEEDEEEDEEDTEMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H716</accession>
    <name>A0A1A0H716_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_44687</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710275.1">
        <property type="nucleotide sequence ID" value="XM_018857829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H716"/>
    <dbReference type="STRING" id="869754.A0A1A0H716"/>
    <dbReference type="GeneID" id="30030805"/>
    <dbReference type="OrthoDB" id="360390at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="313" mass="36817" checksum="F981FCDEDBDEFE36" modified="2016-10-05" version="1" fragment="single">MIELRHKVENERISDPFSYKQYRQLMLYALQNGLQNHPQVWRTRMEYEVLSAEDLINWESAIDSNLSGCQASDEKSTMYNLIATEYPTMQNVLKSLDYLNPTMAQLQQCYAKHKDNFVYSQVIFDRLLASLCADEDWLAIRALYESRLKVPHRQIQDTYDSFSSFVSEHYPQEYTLIMRTASKLLRATERSQRYYEILEQAISDDPNSPEPWIRYMTQLHQYSNGESPYPAFLAVFYRSLFAGSLCKMGDSQWTDVWLVALQFLSKPQMHHSLERKRIATSFVKCYPKFPRAYSELACSLSTEKEVHSLRNHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H730</accession>
    <name>A0A1A0H730_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PFU-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_32894</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710366.1">
        <property type="nucleotide sequence ID" value="XM_018856153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H730"/>
    <dbReference type="STRING" id="869754.A0A1A0H730"/>
    <dbReference type="GeneID" id="30029129"/>
    <dbReference type="OrthoDB" id="10265988at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032473">
        <property type="term" value="C:cytoplasmic side of mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140036">
        <property type="term" value="F:ubiquitin-modified protein reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070314">
        <property type="term" value="P:G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="230"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="6">
        <location>
            <begin position="365"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="7">
        <location>
            <begin position="486"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="769" mass="84213" checksum="5BFE6F20077EAE10" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H762</accession>
    <name>A0A1A0H762_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_79170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710340.1">
        <property type="nucleotide sequence ID" value="XM_018859014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H762"/>
    <dbReference type="GeneID" id="30031990"/>
    <dbReference type="OrthoDB" id="421671at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025638">
        <property type="entry name" value="DUF4336"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33835">
        <property type="entry name" value="YALI0C07656P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="259" mass="28709" checksum="065F8BC720CE7622" modified="2016-10-05" version="1">MYPSPLAVSSRKLTDNVVLSATGFKMKNRVNFGARMALFRFGDSGGLEVNKALELLTGQTTDFNVTHLIVPNSEHTMAVASFKKAFPMLKIIAMESVQLGKGVSPDYVMTSKYGNKKLGAEALREIGVQEPEILENFEFVYLPSNKELVTYHKESKAVFEADILFNLRSDVPMEQFSPATGFPKNYHPFSGMSFPARFLNADSKMGRFLPRKMVNAKASAKGLQAINAWNFDTIVMCHGNVMEKGGKAAFKKVYLSVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7B4</accession>
    <name>A0A1A0H7B4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710441.1">
        <property type="nucleotide sequence ID" value="XM_018856167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7B4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7B4"/>
    <dbReference type="GeneID" id="30029143"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041661">
        <property type="entry name" value="ZN622/Rei1/Reh1_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF8">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR ZNF622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12756">
        <property type="entry name" value="zf-C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="431" mass="49380" checksum="6BF0A5B7DE8F42C0" modified="2016-10-05" version="1">MNYLPQGSNPTTAGFTCNTCGIRFLSAEIQRQHMKTEWHRYNLKRKVAGLPGILSEVFAEKVLASQKSADEEPADEDELGFHINHRRRSGKGERQLTKKDLKQLARGQVRGRITVEDIPSILQRSQSPAASVASEFSEFSLGESVNLSEVESTVDTNSEINYSDDSEWSLIHESDEDSSGTDIEEDHFDEEATHVLPNHYCFYCGKNNHEIEQNIKHMTNRHALYIPERSFLVDLAGLLTFINEVVTVDHDCLVCGFDGKSLESIRLHLTSKGHCRIPYETEEAREVVAEFYNFDTVDRQPLRINSMRKKKSVAFRPASDDSEYEDIGTREDVEDATSDVVLPNGSVIGHRAMVRQQRPNRPMAREWNESERTVALVDRRFAPGLTDFQVSKQEKEMRKLEAKARNVHERMTKPRRVNFQRHFRDEMLGHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7E2</accession>
    <name>A0A1A0H7E2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_208436</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710343.1">
        <property type="nucleotide sequence ID" value="XM_018855013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7E2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7E2"/>
    <dbReference type="GeneID" id="30027989"/>
    <dbReference type="OrthoDB" id="10267305at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd21372">
        <property type="entry name" value="cwf21_CWC21-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051372">
        <property type="entry name" value="CWC21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013170">
        <property type="entry name" value="mRNA_splic_Cwf21_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562">
        <property type="entry name" value="SERINE/ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36562:SF5">
        <property type="entry name" value="SERINE_ARGININE REPETITIVE MATRIX 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08312">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01115">
        <property type="entry name" value="cwf21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CWF21" evidence="9">
        <location>
            <begin position="64"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="20919" checksum="5F293EA8CE4533BB" modified="2016-10-05" version="1">MSSNRIGLQTPRGSGTSGHVQKNIAVAKIEGLREKRKREAAEEHRRMVRAKMTEARKVARAEIESHDQKRQIEVRCMELRESLEDEDLEDEEIDKRVALFRNSLLIEQKAHETKAVPKDESRRLAIEAKEESVKGLKQETKDVSALAVVEEQHETDSRVTDEVCENKGPAPVYDYIPRYDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7R6</accession>
    <name>A0A1A0H7R6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="4">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_13050</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710550.1">
        <property type="nucleotide sequence ID" value="XM_018854295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7R6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7R6"/>
    <dbReference type="GeneID" id="30027271"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000002">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="359"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="530"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="682" mass="76322" checksum="092126A5A2291C0D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7S5</accession>
    <name>A0A1A0H7S5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DUF1682-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45229</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710601.1">
        <property type="nucleotide sequence ID" value="XM_018857893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7S5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7S5"/>
    <dbReference type="GeneID" id="30030869"/>
    <dbReference type="OrthoDB" id="10039147at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032469">
        <property type="term" value="P:endoplasmic reticulum calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012879">
        <property type="entry name" value="CCDC47"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883">
        <property type="entry name" value="ADIPOCYTE-SPECIFIC PROTEIN 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12883:SF0">
        <property type="entry name" value="PAT COMPLEX SUBUNIT CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07946">
        <property type="entry name" value="CCDC47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="327"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="339" mass="39385" checksum="07C8E0AC7CC4A6C8" modified="2016-10-05" version="1">MNLLQRLQLADWRLELFTAFTCVIFFFLYKFGDLYNTKKVSTFLQGVLGVFEENFHLFGVGNGKLFEKDSSENFASYASGRENISRVNLVFRLAPRQNFFIWVMELIFSFFTESVSTPEDKVDIVISPSADYENFISAIVSKLGMNEYRRLNYYLSLTKTSDSASLPESFVFMSEANDFQEKTFTERLAKSLNLSMASYLRYLAFTDQPIDRPQVIRDLIPQRRIVLSVKLVSGKQQLAEISEVLAAVFEIIDKISSGEITFRAESSKKVVKTRDAEVAKIIKAEEAMKQDDLAEEKARLKREEKERFRRMSREEQERAEKKANEKKQRKLQKKMKVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H807</accession>
    <name>A0A1A0H807_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM2-LSM8 complex subunit LSM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM8</name>
        <name evidence="11" type="ORF">METBIDRAFT_78684</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710756.1">
        <property type="nucleotide sequence ID" value="XM_018858919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H807"/>
    <dbReference type="STRING" id="869754.A0A1A0H807"/>
    <dbReference type="GeneID" id="30031895"/>
    <dbReference type="OrthoDB" id="422364at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01727">
        <property type="entry name" value="LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000027">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034103">
        <property type="entry name" value="Lsm8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF9">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="95" mass="10575" checksum="EEA01A1BABFC438A" modified="2016-10-05" version="1">MSALKEYLGKKVRVVTTDARLFEGTLSSFDHSTNVILSDTIERIIYPNEENQMLLLGVYLLRGGSVVCVGEVDELADIDWMTINGEELKGTKNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8I3</accession>
    <name>A0A1A0H8I3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_43893</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710822.1">
        <property type="nucleotide sequence ID" value="XM_018857713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8I3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8I3"/>
    <dbReference type="GeneID" id="30030689"/>
    <dbReference type="OrthoDB" id="1350766at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.30:FF:000001">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000007">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="YbaK/ProRS associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="219"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="633"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="691" mass="75415" checksum="7E1A78B9294F849D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8K1</accession>
    <name>A0A1A0H8K1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 18">3-isopropylmalate dehydratase</fullName>
            <ecNumber evidence="6 18">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Alpha-IPM isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 18">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_32447</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 18">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 18">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="6 18"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710966.1">
        <property type="nucleotide sequence ID" value="XM_018856049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8K1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8K1"/>
    <dbReference type="GeneID" id="30029025"/>
    <dbReference type="OrthoDB" id="2279155at2759"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000006">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000007">
        <property type="entry name" value="3-isopropylmalate dehydratase large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000003">
        <property type="entry name" value="3-isopropylmalate dehydratase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012235">
        <property type="entry name" value="3-IsopropMal_deHydtase_ssu/lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002458">
        <property type="entry name" value="PRK01641.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004016">
        <property type="entry name" value="PRK05478.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009116">
        <property type="entry name" value="PRK12466.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001418">
        <property type="entry name" value="ACN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 18" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="19">
        <location>
            <begin position="11"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="20">
        <location>
            <begin position="542"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00033368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR001418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA20444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="773" mass="84798" checksum="CC0291E369896499" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8K8</accession>
    <name>A0A1A0H8K8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">IMP-specific 5'-nucleotidase 1</fullName>
            <ecNumber evidence="12">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_78672</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">IMP-specific 5'-nucleotidase involved in IMP (inositol monophosphate) degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = inosine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27718"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="3.1.3.99"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ISN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710740.1">
        <property type="nucleotide sequence ID" value="XM_018858914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8K8"/>
    <dbReference type="STRING" id="869754.A0A1A0H8K8"/>
    <dbReference type="GeneID" id="30031890"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006190">
        <property type="term" value="P:inosine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071590">
        <property type="term" value="P:nicotinamide riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071592">
        <property type="term" value="P:nicotinic acid riboside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009453">
        <property type="entry name" value="ISN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28213:SF1">
        <property type="entry name" value="IMP-SPECIFIC 5'-NUCLEOTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06437">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028836">
        <property type="entry name" value="ISN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="467" mass="53011" checksum="638E6BB44C36A008" modified="2016-10-05" version="1">MTSRYRIEYALKSHKRDELIEWIKGLLAVPFVLHGDIDEYEKYMFCHGLPDELKVVSCEQKIEKTLQTRYLEVFHDVEKLIDNTIALDERLPDLDQPARTRLRQLVPSVGRFFTKLPLKEAFLIEDRKRLISRRTLVSPSFNDIRSILNTAQVLALAGAQENDAKLRLVTFDGDVTLYEDGKCLEKDSPLVALLVKLLSLDLYVAVVTAAGYPGQLGADMYQERLLGLIQALRDTNQLLLTQKNNFLVMGAESNYLFRFSSAHGNLRFIEGCEWYLPIMQSWNQDQILEIMDLSHDHLKHLRRKLRHEDENKTTIIRKERSLGIIPVNGYKIQREILEEMVLSILVELNDILSHNKSTANAEKKVQVCAFNGGSDVWVDIGDKALGVEALQLYLYKTCGGTHGKIAKSSSLHIGDQFASVGANDFKARLSACTVWIASPRETTEILEELTNLLENNSGKVNNINLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H903</accession>
    <name>A0A1A0H903_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA N(3)-methylcytidine methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710884.1">
        <property type="nucleotide sequence ID" value="XM_018858936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H903"/>
    <dbReference type="STRING" id="869754.A0A1A0H903"/>
    <dbReference type="GeneID" id="30031912"/>
    <dbReference type="OrthoDB" id="417697at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052735">
        <property type="term" value="F:tRNA (cytidine-3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106217">
        <property type="term" value="P:tRNA C3-cytosine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000221">
        <property type="entry name" value="Methyltransferase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013217">
        <property type="entry name" value="Methyltransf_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026113">
        <property type="entry name" value="METTL2/6/8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22809:SF11">
        <property type="entry name" value="TRNA N(3)-METHYLCYTIDINE METHYLTRANSFERASE METTL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08242">
        <property type="entry name" value="Methyltransf_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037755">
        <property type="entry name" value="Mettl2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 12" evidence="6">
        <location>
            <begin position="125"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR037755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="331" mass="38452" checksum="4AED59FC9306757A" modified="2016-10-05" version="1">MESKPEQPTEPKPALESRIGKDSPFTFGQRLLTEDKDVFDHNAWDHVEWGQEQIDDAEKLIAKQYEQPVKDFDKKLYNASPAKFWDIFYRNNRENFFKDRKWLQIEFPFLYEATGAEYNKPVSILEVGCGAGNTFYPILNQNQNPQLKIWGCDYLKVAVDLVRNNENFAPNNDKGIAYSSVWDLANAEGQIPDDMEPHSVDVVIMVFVFSALSPEQWRNAIQNLDKVLKPGGQILFRDYGRYDLAQVRFKKGRLLEDNFYIRGDGTRVYFFTEEELRTIFCTEGPFSETNIATDRRLLVNRKKQLKMYRIWLQAVFHKPEGSGSVSGDDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H976</accession>
    <name>A0A1A0H976_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Cytochrome c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_44371</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="12">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome c family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711093.1">
        <property type="nucleotide sequence ID" value="XM_018857785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H976"/>
    <dbReference type="STRING" id="869754.A0A1A0H976"/>
    <dbReference type="GeneID" id="30030761"/>
    <dbReference type="OrthoDB" id="449280at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.760.10:FF:000001">
        <property type="entry name" value="Cytochrome c iso-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="entry name" value="Cytochrome c-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002327">
        <property type="entry name" value="Cyt_c_1A/1B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11961">
        <property type="entry name" value="CYTOCHROME C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00604">
        <property type="entry name" value="CYTCHRMECIAB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="Cytochrome c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 10 12" id="KW-0349">Heme</keyword>
    <keyword evidence="9 10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
            <begin position="8"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="110" mass="12080" checksum="773F31D82303A3E9" modified="2016-10-05" version="1">MPAPFEKGSEKKGATLFKTRCLQCHTVEAGGPHKVGPNLHGIFGRKSGLAEGYSYTDANKKKGVEWSEQTMSDYLENPKKYIPGTKMAFGGLKKPKDRNDLITYLASATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9F1</accession>
    <name>A0A1A0H9F1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Target of rapamycin complex subunit LST8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_44169</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711025.1">
        <property type="nucleotide sequence ID" value="XM_018857751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9F1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9F1"/>
    <dbReference type="GeneID" id="30030727"/>
    <dbReference type="OrthoDB" id="400at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000339">
        <property type="entry name" value="WD-repeat protein pop3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="76"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="117"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="160"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="208"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="308" mass="34522" checksum="C98D7DE8EC668625" modified="2016-10-05" version="1">MSVILASAGFDHTIRFWDALTGVCSRTIQHTDSQVNRLEITSDKRFLAAAGNLYVRLYDIKQAGNTGGNPAPAMTFEGHTGNVTSLAFQTDNRWMVSSLEDGTVKVWDVRSPSVQRDYKHFCPVNEVVIHPNQGELISCDHDGNIRIWDLGENQCTHHLIPEDDVPINSLSVASDGSMLVAGNNKGNCYVWEMQNKKDVTSLMPATKFRSHLKYITRVVLLTDMKHLATCSADHTARIWLTKDNFALETTLQGHQRWVWDCTFSADSAYLVTACSDHYVRLWDLSNSDTVRHYNGHHKGALCVALNDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9S4</accession>
    <name>A0A1A0H9S4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">PTR2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_32715</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a dipeptide(out) + H(+)(out) = a dipeptide(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64392"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:90799"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:64393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an L-amino acid tripeptide(out) + H(+)(out) = an L-amino acid tripeptide(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:64400"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:155837"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64401"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711288.1">
        <property type="nucleotide sequence ID" value="XM_018856116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9S4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9S4"/>
    <dbReference type="GeneID" id="30029092"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071916">
        <property type="term" value="F:dipeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000085">
        <property type="entry name" value="MFS peptide transporter Ptr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="256"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="26"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="582" mass="65522" checksum="C959389B8F2D5F37" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9S7</accession>
    <name>A0A1A0H9S7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NIF-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32703</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711272.1">
        <property type="nucleotide sequence ID" value="XM_018856114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9S7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9S7"/>
    <dbReference type="GeneID" id="30029090"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000043">
        <property type="entry name" value="General stress response phosphoprotein phosphatase Psr1/2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="172"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="346" mass="37916" checksum="37E83BB1DA7E5F62" modified="2016-10-05" version="1">MGLFASFFCCGADTSSDSEDKHHKPSSARSSPASRTGGKHQNTKMTQKTAVSEAAHKSAAKVVSPGAAKVVPDDDNSVNESDTLSVPNDEPSKLNPAKPQNAAAAETPAEPTNPVHSDIVASVPENPADAEPAAEAALHNHEAPAFDLSAIQENQAFNPETGFLLGKKDTKRFGNKKCLVLDLDETLVHSSFKYLRTADFVIPVEIEDQVHHVYVIKRPGVDEFLRKVGQWYEVVVFTASVAKYGDPLLNKLDIHHAVHQRLFRDSCFSYQGNFIKNLSQLGRPLEDLIIIDNSPALYIFHPQHSVPISSWFSDTHDNELLDLLPLLEDLSRPNVDDVSLVLDITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA09</accession>
    <name>A0A1A0HA09_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_203441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711234.1">
        <property type="nucleotide sequence ID" value="XM_018854964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA09"/>
    <dbReference type="GeneID" id="30027940"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="80" mass="9440" checksum="B4D2502E731A2886" modified="2016-10-05" version="1">MDWQYHSPTQKNASNSSDSFLTQKNQGLTRTNIQPNTHDHPWHRISSLVLLKPKYRKYHNWNPEFSQQNTKTGTRTFPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA47</accession>
    <name>A0A1A0HA47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_42743</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711514.1">
        <property type="nucleotide sequence ID" value="XM_018857580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA47"/>
    <dbReference type="STRING" id="869754.A0A1A0HA47"/>
    <dbReference type="GeneID" id="30030556"/>
    <dbReference type="OrthoDB" id="17410at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036033">
        <property type="term" value="F:mediator complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006972">
        <property type="term" value="P:hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000879">
        <property type="term" value="P:negative regulation of dipeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042304">
        <property type="term" value="P:regulation of fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000111">
        <property type="entry name" value="Transcriptional repressor rco-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013890">
        <property type="entry name" value="Tscrpt_rep_Tup1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08581">
        <property type="entry name" value="Tup_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcriptional repressor Tup1 N-terminal" evidence="8">
        <location>
            <begin position="26"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="290"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="325"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="366"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="415"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="475"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="517"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="101"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="557" mass="61666" checksum="353FE2D8CF73B279" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA62</accession>
    <name>A0A1A0HA62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_230331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711285.1">
        <property type="nucleotide sequence ID" value="XM_018855247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA62"/>
    <dbReference type="STRING" id="869754.A0A1A0HA62"/>
    <dbReference type="GeneID" id="30028223"/>
    <dbReference type="OrthoDB" id="4073963at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="302" mass="33786" checksum="52197D62DF0A8E70" modified="2016-10-05" version="1">MRGSEDDWWFKFQNDKPGIPQAQVPGENRADDQSKRTLVKRFLHSLMYKVKERVDPPRNFSLNTLKASASVSGTMLKQLDQSLLISRSQLTNRRILCVAGLPATTSMATTMSLVCGGGLEKVVFHDGHSPTVEVHFLSPESAHKFHEYSERLPLLAINGYPLSVTWAKPTRGLPAGHAPLASYVAEEVEGYRASRVIVLTRPVPKRQTDTAASRGYPDARDNYSADLNIEKVKWDFVQFGGIVEVMPMILSKLSISIQYTDIRSAILAMHTLSQKSSLLYSKYSLWSAKYVRDVTNRPCLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAK3</accession>
    <name>A0A1A0HAK3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SRR1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711551.1">
        <property type="nucleotide sequence ID" value="XM_018855893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAK3"/>
    <dbReference type="GeneID" id="30028869"/>
    <dbReference type="OrthoDB" id="551431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012942">
        <property type="entry name" value="SRR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040044">
        <property type="entry name" value="SRR1L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28626:SF3">
        <property type="entry name" value="SRR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07985">
        <property type="entry name" value="SRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRR1-like" evidence="2">
        <location>
            <begin position="35"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="252" mass="28550" checksum="7EF84DA18CCA120C" modified="2016-10-05" version="1">MESTRFESLAAKLQKHEQCVAQSDLYTHTVTLLRPLKFTMIRCLALGSPTQEFQALYQLAYLKLLASSCNIEAKNISCYDPAFLAEDTQFLANALGYVVEAPEEYCPGPETTGSTLYYMPHAPRTLTESVLAQHRPRWLLANDFSVTMGTLTKARFLQECPTLATLVHLFDQNTAQKLAEPAQEDGFVRVVSRKRRSRPRKNVYVEPELEYDVPGKYFRDVHISRIKADAGAPWRDSFSDLALNVVVPKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAT6</accession>
    <name>A0A1A0HAT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="8">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_41625</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711508.1">
        <property type="nucleotide sequence ID" value="XM_018857435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAT6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAT6"/>
    <dbReference type="GeneID" id="30030411"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF46">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE HMI1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 10 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="12">
        <location>
            <begin position="146"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="13">
        <location>
            <begin position="450"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="167"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="770" mass="82907" checksum="CCDB3BC196DA53E0" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB08</accession>
    <name>A0A1A0HB08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UPF0052-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711706.1">
        <property type="nucleotide sequence ID" value="XM_018857480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB08"/>
    <dbReference type="STRING" id="869754.A0A1A0HB08"/>
    <dbReference type="GeneID" id="30030456"/>
    <dbReference type="OrthoDB" id="10267139at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31240:SF0">
        <property type="entry name" value="MATERNAL EFFECT EMBRYO ARREST 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="CofD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="459" mass="50654" checksum="EB5C4E5CB8BBE5FC" modified="2016-10-05" version="1">MTISTVILSGGTATNDLVPLFSRSDFKVSYVLPILDTGGSSSEIIRVIGGPAIGDIRSRLIQLIPHHQRRLRDVLSLRLNANASVAKAQWEQIVDGSHELWHGIPNSTKEIVRSFLLHIHVELLKRSRIYAGSSPGKQFKFEFANIGNLLLTAIRLFTGSLDSAIELFMKITDIDSSSEVLPCVNTNFSYHISAFLSDGSTITGQSQISHPSMKSIYSNSKSSIESSSEPDTFGPDQQIDGLDEFHDFDDANIPSDEEIGNTPLYTHPDLKKSQLHFRKTSEIEPLLSPIKRIFYVSPYGEEICPTAQSRVLSKLLHAEIIVYSIGSLMTSITPIVILKGVGRAIAENPTKFKKRILLLNGNEDRETAGLGALDYIRVIVELAQYLIKKSGHGQVLDWTLFVTHLFYMENSKIPVDIEALENIGITCVAIKQATDGIDKYDLEDLSLNFLKLSGTGAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBA4</accession>
    <name>A0A1A0HBA4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NUDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_41973</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nudE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711667.1">
        <property type="nucleotide sequence ID" value="XM_018857473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBA4"/>
    <dbReference type="STRING" id="869754.A0A1A0HBA4"/>
    <dbReference type="GeneID" id="30030449"/>
    <dbReference type="OrthoDB" id="5877028at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF1">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="38315" checksum="B6345E790790D182" modified="2016-10-05" version="1">MDAVEFEKVYLRMLELELELVEFQESSKDLELALEDELHDLETQKSTLVHQLLSKDKQISMLNSRVISLNSEVTGLLELLVETKSAYEETIADLKHKLVAMEILNDDFLSRDRVVESKLQLANQFNNELLEKLAMVENDLDLERQANAQHKLTISNLSNITAHQPVRLTRSKRDSTFRDFAFAESTVLDIDEMLATEPPAPMVEAEFPRSESLTRFQELCSKSDILRQKVGEVNTSLAFKSLSTAEVSKPMALSFPSEGLTSFASAISDVGEAQPITEKTRKKEARRSVSEKGKDRLSLQATQTRLDDGRHKGMHVIPELGRGSSKKFKLRNVMKSFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBG2</accession>
    <name>A0A1A0HBG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711835.1">
        <property type="nucleotide sequence ID" value="XM_018857406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBG2"/>
    <dbReference type="STRING" id="869754.A0A1A0HBG2"/>
    <dbReference type="GeneID" id="30030382"/>
    <dbReference type="OrthoDB" id="303107at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="349"/>
            <end position="386"/>
        </location>
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            <end position="535"/>
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        <location>
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            <end position="377"/>
        </location>
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        <location>
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            <end position="472"/>
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        <location>
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            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="535" mass="60095" checksum="08E2811AF7E095E4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBN8</accession>
    <name>A0A1A0HBN8_9ASCO</name>
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        <recommendedName>
            <fullName evidence="7">SAGA complex subunit Spt7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_78302</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
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                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
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                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
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                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12 13">NRRL YB-4993</strain>
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            <location evidence="1">Nucleus</location>
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        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BROMODOMAIN"/>
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        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 8" id="KW-0103">Bromodomain</keyword>
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    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="365"/>
            <end position="435"/>
        </location>
    </feature>
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    <feature type="region of interest" description="Disordered" evidence="10">
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        <location>
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            <end position="1069"/>
        </location>
    </feature>
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        <location>
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        </location>
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        </location>
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            <dbReference type="EMBL" id="OBA21292.1"/>
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    <sequence length="1069" mass="122770" checksum="2FFBF18A29721760" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBY9</accession>
    <name>A0A1A0HBY9_9ASCO</name>
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            <fullName evidence="2">F-box domain-containing protein</fullName>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Hittinger C.T."/>
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                <person name="Salamov A."/>
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                <person name="Barry K."/>
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                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
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                <person name="Slot J.C."/>
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                <person name="Sun H."/>
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                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="321"/>
        </location>
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        <location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        <location>
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            <end position="56"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="235"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="900" mass="100683" checksum="C2C939D0ED5C3A9E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC13</accession>
    <name>A0A1A0HC13_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription factor TAFII-31</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="ORF">METBIDRAFT_41552</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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            <taxon>Pichiomycetes</taxon>
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            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712184.1">
        <property type="nucleotide sequence ID" value="XM_018857423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC13"/>
    <dbReference type="STRING" id="869754.A0A1A0HC13"/>
    <dbReference type="GeneID" id="30030399"/>
    <dbReference type="OrthoDB" id="341924at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="161" mass="17745" checksum="B3076199CBE3C04A" modified="2016-10-05" version="1">MTQNGPSLSGATQASEPAVVPRDVRLLHLIFATQGVQNYQDHVPLQLMDFAHRYTSAVLKDAVTYGDHARGSNGSSQATAAVNTDDIRLAIAAKTNYQFKPAPAKELMLELAHERNGKSLPPVIPKWGLNLPPEKYCLTAKDWDSLDEERGDLDASKRRKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC35</accession>
    <name>A0A1A0HC35_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dipeptidyl peptidase IV/CD26, N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32053</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712055.1">
        <property type="nucleotide sequence ID" value="XM_018855976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC35"/>
    <dbReference type="STRING" id="869754.A0A1A0HC35"/>
    <dbReference type="GeneID" id="30028952"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF160">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="9">
        <location>
            <begin position="244"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="8">
        <location>
            <begin position="713"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="916" mass="103502" checksum="858A6125A4FB40BE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC42</accession>
    <name>A0A1A0HC42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptide hydrolase</fullName>
            <ecNumber evidence="9">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_31976</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family. M28A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711968.1">
        <property type="nucleotide sequence ID" value="XM_018855955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC42"/>
    <dbReference type="STRING" id="869754.A0A1A0HC42"/>
    <dbReference type="GeneID" id="30028931"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03876">
        <property type="entry name" value="M28_SGAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000093">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041756">
        <property type="entry name" value="M28_SGAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF17">
        <property type="entry name" value="AMINOPEPTIDASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444435" description="Peptide hydrolase" evidence="9">
        <location>
            <begin position="18"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="10">
        <location>
            <begin position="163"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="11">
        <location>
            <begin position="276"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="525" mass="56842" checksum="2CDE4306DA8F3B39" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC94</accession>
    <name>A0A1A0HC94_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_87680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712125.1">
        <property type="nucleotide sequence ID" value="XM_018859284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC94"/>
    <dbReference type="STRING" id="869754.A0A1A0HC94"/>
    <dbReference type="GeneID" id="30032259"/>
    <dbReference type="OrthoDB" id="6509908at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032218">
        <property type="term" value="P:riboflavin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF177">
        <property type="entry name" value="RIBOFLAVIN TRANSPORTER MCH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="52"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="359"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="5">
        <location>
            <begin position="52"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="460" mass="48540" checksum="0629AD0975BDA398" modified="2016-10-05" version="1">MASDSIELRSTGHGSTTKTRGNDTNTRGNDTNTSGNDTNTSGNSYPEGGWKAYLVVLGSHLGLMVHFGILNAVGAVQAYVSSHQLAGEAVSSVSWVFSIYMCLPFFLGALVGPVFDRRGSKQLLAASTALLFAGFLALSFSLLIVAFLFSLSLCLGTAHALAIPPLVSLLSHWFLVHRGKALGLASLGGSVGGTIWPLVLQALYGSVGYAWAIRVVGAVAVCLLAASVFLVELRFTLRTHSPPEQAASKTRRLAASMRRHLDVSAFREPRFAALVAGVFLTEIALLSVVTYLASYALAHGFSEKHSLLLLTLLNAAGIPGRYLLGLMADRYGSVNTMVVMLSGFCVSIFAVWLPGGGSAALWGFAAFFGFCSSSILSLTPVCLGLFTPVAVFGRCYGLMYTFSSTGILFGIPVGSAIIDDSSVASYRNFTVFCGCFAVAGTVSWGVCRYLLVGARLNVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCD7</accession>
    <name>A0A1A0HCD7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein FYV4, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_22050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712166.1">
        <property type="nucleotide sequence ID" value="XM_018855136.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCD7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCD7"/>
    <dbReference type="GeneID" id="30028112"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01238">
        <property type="entry name" value="IGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS41 SAM" evidence="6">
        <location>
            <begin position="42"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="152" mass="17687" checksum="B31F96FFD69DBA80" modified="2016-10-05" version="1" fragment="single">STLLRPSPAVRLLAAGPRMFSSSAFAWKTNTSTRTKENVHDLETFFKLIGRDTIEHLDAFDGKLNKFLRTTSKLMKTKGIDVSTRRYMLRWRHKFINNLEPLREHKKGKKKNGGERKAKAVKAKKIALERAEEKERFIAEELEAERKGERQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCE5</accession>
    <name>A0A1A0HCE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_32060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712067.1">
        <property type="nucleotide sequence ID" value="XM_018855979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCE5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCE5"/>
    <dbReference type="GeneID" id="30028955"/>
    <dbReference type="OrthoDB" id="1731724at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01452">
        <property type="entry name" value="VWA_26S_proteasome_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000005">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027040">
        <property type="entry name" value="PSMD4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10223:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="4">
        <location>
            <begin position="5"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="226"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="269" mass="28813" checksum="5728FE1DA01CB593" modified="2016-10-05" version="1">MVLEATMIAIDSSEFMRNGDFLATRYDAQITAMEFVFQNKLNANPENTVGLLSYGGNGPQVLSTLTTDFGKILSGAHGTRISGDSHFASGIQVAALALKHRQNKVQSQRIIAFVGSPLRESDKDLDRLAKKMKKNNVAVDIVNFGEESANTAKLERFHAAVNNHDNSHLVTVPPGPRLLYEVIASSPILVEDGFDVGDTGDFLGMGAVDANMDPDLALALRLSLEEEKQRQEREAADGASPPADLGRIAEEAAGPDDAKHDDAKPSGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCH3</accession>
    <name>A0A1A0HCH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_188413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712097.1">
        <property type="nucleotide sequence ID" value="XM_018854815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCH3"/>
    <dbReference type="GeneID" id="30027791"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="156" mass="17305" checksum="C55FE0B709A7FBB8" modified="2016-10-05" version="1">MIWHGCENQTVANLKLAPGARLGGFSLLFLASEYFGCPFLRFGFLLSVTFLARVWFLVSCGPSKKRGCIIKTLVYTNGPKSSYSLQIWGQSSLIEGKYKLERQPGQKKAPTFPQPLGTGFQGLVCLCSTVMLISPNFLHLKISTKSCERVYETNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCP1</accession>
    <name>A0A1A0HCP1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oxidative stress survival, Svf1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41394</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712172.1">
        <property type="nucleotide sequence ID" value="XM_018857394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCP1"/>
    <dbReference type="GeneID" id="30030370"/>
    <dbReference type="OrthoDB" id="2590239at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051385">
        <property type="entry name" value="Ceramide-binding_SVF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033394">
        <property type="entry name" value="Svf1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013931">
        <property type="entry name" value="Svf1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107:SF1">
        <property type="entry name" value="CERAMIDE-BINDING PROTEIN SVF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107">
        <property type="entry name" value="SVF1-LIKE PROTEIN YDR222W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08622">
        <property type="entry name" value="Svf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17187">
        <property type="entry name" value="Svf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159245">
        <property type="entry name" value="AttH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Svf1-like N-terminal" evidence="4">
        <location>
            <begin position="58"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Svf1-like C-terminal" evidence="5">
        <location>
            <begin position="222"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="41280" checksum="C276904341F1390A" modified="2016-10-05" version="1">MLKWMQSGLSVVAGTAEPEYGREAIHPVTDFIKQQGQAVSRATVLEDFAWKHPNYTNVETQTFYFTDLQSGYIGLAQVIHSNLMGIKTTAQFTFRLYNSKLGAADPNCVWTSTALENFRTDGANFYADNLSIENSGANNDTYTLKLAVTEDSVVDLVVTRIVPGVVFGADGTTFYGDSLDEPWGSMRHAFWPRCAVTGIIKLKAATVEISGYTMFVMALQGMKPHHAAKSWNFLNFQSEHYSAVQMEFTTPKSYATTKVNVAILTSDTEILATSIDNDVVHENAEVDEVGWSVPKSITFNFSEGTSAAPVATVSGALSQLVERVDVMAEIPQFVKNIVSGVAGAKPYIYQFCNTLTIKHGDDSEDGVAFNEATFISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS8</accession>
    <name>A0A1A0HCS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712233.1">
        <property type="nucleotide sequence ID" value="XM_018857400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS8"/>
    <dbReference type="GeneID" id="30030376"/>
    <dbReference type="OrthoDB" id="15108at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="80" mass="9290" checksum="A738303E163BEC39" modified="2016-10-05" version="1">MAGTLRPDAEFQRFNTAKEKLGHYFRFTTRSALFNIVFAGLVPVGLTIMAYNQEGQYPFARVFRNDVILDQEYVPRKKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCV7</accession>
    <name>A0A1A0HCV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(32) synthase</fullName>
            <ecNumber evidence="3">5.4.99.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_77402</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(32) in tRNA = pseudouridine(32) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42544"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10108"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.28"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.28" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712337.1">
        <property type="nucleotide sequence ID" value="XM_018858692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCV7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCV7"/>
    <dbReference type="GeneID" id="30031668"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160151">
        <property type="term" value="F:tRNA pseudouridine(32) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02557">
        <property type="entry name" value="PseudoU_synth_ScRIB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000061">
        <property type="entry name" value="DRAP deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000017">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18785">
        <property type="entry name" value="Inv-AAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="7">
        <location>
            <begin position="422"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00036184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="585" mass="65663" checksum="548BB295CD211215" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCW9</accession>
    <name>A0A1A0HCW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_41476</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">High-conductance magnesium-selective channel that mediates the influx of magnesium into the mitochondrial matrix. Essential for the splicing of mRNA group II introns in mitochondria by affecting mitochondrial magnesium concentrations, which are critical for group II intron splicing. It also suppresses a variety of mitochondrial intron mutations and its absence may disturb the assembly of mitochondrial membrane complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homopentamer. Forms homooligomers. Interacts with MFM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Mitochondrion inner membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712237.1">
        <property type="nucleotide sequence ID" value="XM_018857408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCW9"/>
    <dbReference type="GeneID" id="30030384"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901612">
        <property type="term" value="F:cardiolipin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF27">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00046105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="389" mass="44295" checksum="B28F4E3E9D70A98F" modified="2016-10-05" version="1">MASPIAPNDLYVSYTTFDTEGNVTAVSQKIPKAQFLNENNLFPRDLRKVDSSSIDVAPTIMVRSENAIIVNLLHIKAIIKNDNIKIFDTANPSVASKLGLFMYDLELKLKTPSSLPYEFKALECILISVLSYLEAQLQTHLTSCGKILAELEDNINRESLQELLIKLKKLSGFFQRATLIRDVLEELLDNDEDLNGMYLTKGLKYNPSDTALDYSEIEMILETYYQHCDEVVQQAGSLISDIKATEEIANIILDANRNSLMLFELKVTIYTLGFTVATLIPAFYGMNLKNYIEDSFYGFGAVVAMSIVQGLLITYWNFKKLHKVQTLTMMASPGTTTKNRLQFYGLEGGWPRKQWLSRLLFGRRAKQLPPTREERDVIWRMIKNDKNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCX0</accession>
    <name>A0A1A0HCX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_24535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712269.1">
        <property type="nucleotide sequence ID" value="XM_018855281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCX0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCX0"/>
    <dbReference type="GeneID" id="30028257"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="363" mass="37276" checksum="F78A6AD41C6A41A5" modified="2016-10-05" version="1" fragment="single">VFAVTITENTVQISTIDITLGDLTINAGVYYSIVNSALVTLAGSVTNAGGFYVTSANGLAASVVMTGSSFVNSGTCAFNSLSATVLSTYDIATLGSFLNTGDMYFGISGATIVGTPFIVTSVTSWSNDGMMVFRRASGDSALLVIEQVVGSGGLSTILNDGSICLYNTYWLQTTSIVGSGCITVGSGSEMQLQLSVGTLLFSVAESQTIYLASSDSVLSILGLSLSLLPDNTITVAGFGNGNKIELDILFLSYTYSSTTGILRLTLAILLSVEIYIGPGYNSLYFSTASTLLSKSISYSRSPPNAAPAICACSYNFPEVTTTALSSSTSTTSVNSDGSVETASGVVIVNTDSAGVVTTTTSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD59</accession>
    <name>A0A1A0HD59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_22911</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712364.1">
        <property type="nucleotide sequence ID" value="XM_018855219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD59"/>
    <dbReference type="STRING" id="869754.A0A1A0HD59"/>
    <dbReference type="GeneID" id="30028195"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="17"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="304" mass="34632" checksum="6D0C826D2E6000C7" modified="2016-10-05" version="1" fragment="single">TRQERIDEARYTSQLNLDKIDEIFGVYGRNLTKVVDLGSAPGTWLRHTRDRLLGLHGLKPEKIYEKCTVIGVDLLFCQPPRGTFFTQGNMYSQNTHTQIETLLKEAAFRIARPKRRDIGTEFDVSYILRELNQVQLEANIATEKTAQLTKATKQTSDCYDLPPYAHQADVVLSDLGTPPLQESGFHINTLSKPFIRSSLNNMLRQPWDNPEKSSIDLAEAALLLSGNILAKGGTFVVRLSMVNLADPELELLEARLRMVFHRVTRWSDNGRTTSTEHRQQNLFMVCKDKKDHLMDKYAVFGVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD65</accession>
    <name>A0A1A0HD65_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712369.1">
        <property type="nucleotide sequence ID" value="XM_018855730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD65"/>
    <dbReference type="GeneID" id="30028706"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508822" description="Secreted protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11109" checksum="914304C773BE684C" modified="2016-10-05" version="1">MCPGSKLLSLKVVFFFALSSLPLLSLFSSPKVRILACFHRQTGCPILFLARTQIWATSRNLAFSHAAIDNKHDIGHFRPLFLASTCDRCRKAVSGPISSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDH4</accession>
    <name>A0A1A0HDH4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_143596</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712474.1">
        <property type="nucleotide sequence ID" value="XM_018854500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDH4"/>
    <dbReference type="GeneID" id="30027476"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="118" mass="13382" checksum="A27EA1597D9E7CC3" modified="2016-10-05" version="1">MRRWIWARYRARRLWLGFRSRAPSHSIQTNMSCMVARGGRRTAVSGKSGDGPKGPSAVEKTGASYELHIRRALRFLALAVFSNSAFSTRPWFQDWRAGSSPAKWLRRCRPSEGSLISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDI9</accession>
    <name>A0A1A0HDI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712577.1">
        <property type="nucleotide sequence ID" value="XM_018855758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDI9"/>
    <dbReference type="GeneID" id="30028734"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8654" checksum="0947AFD1335E4332" modified="2016-10-05" version="1" fragment="single">MPDILGSNISNDVWKFQGRKRRNSRKRPERSVTMATTTESTLTDQKGSLGSTPPMKQPSSNLNPFLVLAEISEEDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDQ2</accession>
    <name>A0A1A0HDQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31021</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712549.1">
        <property type="nucleotide sequence ID" value="XM_018855755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDQ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDQ2"/>
    <dbReference type="GeneID" id="30028731"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="310" mass="34391" checksum="7FD0C6747E9E1645" modified="2016-10-05" version="1">MRSQDIIIGGFGILIDAYFAIVNSAFVRTDGSVTFDGDFYIINFDKSSVELDMGGDLFLNFANFTLDSLLAESEPRVLIYYTNVFNNGDMFFGDSGNHSRALSIRASEILSNKGMMVFKRASGDKLQLNLGSTTHRHSILKNSGSICLYNTSWKIPKNIEKHGCITVGTGSILDFLLRYYDYISPFDQIIYLESDSEVRISGLKSPLATIPSIEVVGWSEDNKIILDTVIESTEKLVYSEDTGILSIFGTAEPIITLNIGKGYWGAAFRLLLDDYGSTLQYWMSVLGASRPSKCRCVTEFPKVPTTRPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW4</accession>
    <name>A0A1A0HDW4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_11042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712666.1">
        <property type="nucleotide sequence ID" value="XM_018853906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDW4"/>
    <dbReference type="GeneID" id="30026882"/>
    <dbReference type="OrthoDB" id="4096756at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="567"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="625"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="468"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="516"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="653" mass="69467" checksum="958FEA406684C454" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ5</accession>
    <name>A0A1A0HDZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sensitive to high expression protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_18948</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the maintenance of the structure of the mitochondrial inner membrane. Involved in mitochondrial morphology. Causes growth arrest when highly overexpressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the SHE9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712624.1">
        <property type="nucleotide sequence ID" value="XM_018854825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDZ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDZ5"/>
    <dbReference type="GeneID" id="30027801"/>
    <dbReference type="OrthoDB" id="5595506at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008839">
        <property type="entry name" value="MDM33_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31961">
        <property type="entry name" value="SENSITIVE TO HIGH EXPRESSION PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31961:SF3">
        <property type="entry name" value="SENSITIVE TO HIGH EXPRESSION PROTEIN 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05546">
        <property type="entry name" value="She9_MDM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="21"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="32177" checksum="7B2E9D499B2B5F36" modified="2016-10-05" version="1" fragment="single">VQDSLRLAARSLNDATGYSGIEKHKLLVEQVERDIKQAREAVKIAKASYERAIQNRSDLQKEINELLTRKHTWSPIDVERFTELYKNDHRNQHDEVSSKLALDEAEQNVEAVQIKLTALILTRYHEEQLWSDKIRQALTWGTWMLMGVNIMLFATATFFVEPWKRRKLVNAFQAEVQLKMEEYSAELKSLSQQLSPETADARGTEESVPPETLVLDIETGTHRISFSEITSWKTLQNWVSAFVAAFKVPENTNFSMERPDFAIFSGILVAFGWLFGSLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE86</accession>
    <name>A0A1A0HE86_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4 9">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_150681</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:13877"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.13" evidence="4 9"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712802.1">
        <property type="nucleotide sequence ID" value="XM_018854562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE86"/>
    <dbReference type="STRING" id="869754.A0A1A0HE86"/>
    <dbReference type="GeneID" id="30027538"/>
    <dbReference type="OrthoDB" id="10261433at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00358"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004587">
        <property type="term" value="F:ornithine aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019544">
        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006591">
        <property type="term" value="P:ornithine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000011">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000152">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010164">
        <property type="entry name" value="Orn_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01885">
        <property type="entry name" value="Orn_aminotrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="430" mass="46600" checksum="53D23608AF79E972" modified="2016-10-05" version="1">MSISSATAKQYEEQYSAHNYHPLPVVFQKAKGAHVWDPEGKEYLDFLSAYSAVNQGHCHPKIIEALVEQASKLTLCSRAFSSDVFGEYAKYVTEFFDYEMVLPMNTGAEAVETGLKLARRWGYMKKGIKEDEAIILSAVDNFHGRTLGVISMSTDPDATTDFGPYLSGVGPQIPGESKGTLLRYGVIEDVEKAFSNAGDKIAALLLEPIQGEAGIVVPPQGYFTRVQELCRKHNVLLICDEIQTGIARTGKMLCYEHYDNVKPDIVLLGKAISGGVYPVSAVLSSKDIMLTLTPGCHGSTYGGNPLACKVAMAALQVVKDEGLVERAEKLGELLRSKLEALKAESGGIISEVRGKGLLSAIVIDDSKANGRTAWDLCLVMKDYGVLAKPTHDHIIRLAPPIVISEDDLLKGVDVIRKSLADLPNAPHAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEH0</accession>
    <name>A0A1A0HEH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal lysine N-methyltransferase 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_39159</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. RKM5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713005.1">
        <property type="nucleotide sequence ID" value="XM_018857105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEH0"/>
    <dbReference type="GeneID" id="30030081"/>
    <dbReference type="OrthoDB" id="2529286at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF109">
        <property type="entry name" value="RIBOSOMAL LYSINE N-METHYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="310" mass="33974" checksum="5CAB9E923072961B" modified="2016-10-05" version="1">MINAAALLEISHESLYEHVFAAHTEHRPCAHQHLGYVDKSADKINVCLPRAGVDLSVQQLLLSLSSGTQLSSTGFICWQTALYFADWAVAFAPCPLAAVLHKDSVVLELGAGVSAVLAAVVGPRVGHYVASDQAHLLKLMKQNFRANVVSQRYDSNTCAKDRDHAPRRLDAAAKWSQIDFVALDWEGPGPGTRRFAELTGRPHADVVVACDTIYNSYLIAPFLACMALAMCEHTVAVVAMQLRDEAITEEFLERALQAGLRVFQVRDEHLTPELIEGFAVYCFRKRKAEGQAEGTCGEHIRGGHIWGDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEH2</accession>
    <name>A0A1A0HEH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712881.1">
        <property type="nucleotide sequence ID" value="XM_018857081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEH2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEH2"/>
    <dbReference type="GeneID" id="30030057"/>
    <dbReference type="OrthoDB" id="360689at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047865">
        <property type="entry name" value="Ribosomal_uL10_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="25630" checksum="2DFA71D53CAE4669" modified="2016-10-05" version="1">MAPAQNFSTTRRACQQEQPIETYPVDLARKTTKHVLSRKTFLVDYYKYLNDNNEIVLYAHHNNLLKNDTLKVRADLKKLGAKLTYVRNNLYGVYLRSENEADPALHKNTLKNKKKNHPLEQLLNGPTAVITIPLCDPAGVALVMKVLKQAGEKLFLIGARIETGVYSAAQVEEFKTLPSKDELRGQLAGLLSVLGGAGLVRTLESAGTGLYLTLGQREKDLGGDDDASPLDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEK1</accession>
    <name>A0A1A0HEK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_77825</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCB family. Mitochondrial peptide exporter (TC 3.A.1.212) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712917.1">
        <property type="nucleotide sequence ID" value="XM_018858775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEK1"/>
    <dbReference type="GeneID" id="30031751"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090374">
        <property type="term" value="P:oligopeptide export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18573">
        <property type="entry name" value="ABC_6TM_ABCB10_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000218">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="119"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="457"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="55"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005580"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="707" mass="76506" checksum="447B54CAD77EF4BA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEZ5</accession>
    <name>A0A1A0HEZ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">FCH-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_35054</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713175.1">
        <property type="nucleotide sequence ID" value="XM_018856566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEZ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEZ5"/>
    <dbReference type="GeneID" id="30029542"/>
    <dbReference type="OrthoDB" id="27823at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="10">
        <location>
            <begin position="6"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="9">
        <location>
            <begin position="609"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="321"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="405"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="321"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="405"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="677" mass="76475" checksum="6A30AC257710715B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF36</accession>
    <name>A0A1A0HF36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Histone deacetylase 8</fullName>
            <ecNumber evidence="6">3.5.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Protein deacetylase HDAC8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Protein decrotonylase HDAC8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_34419</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the histone deacetylase family. HD type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713225.1">
        <property type="nucleotide sequence ID" value="XM_018856470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF36"/>
    <dbReference type="STRING" id="869754.A0A1A0HF36"/>
    <dbReference type="GeneID" id="30029446"/>
    <dbReference type="OrthoDB" id="73273at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF14">
        <property type="entry name" value="HISTONE DEACETYLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="19">
        <location>
            <begin position="24"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA22744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="372" mass="40838" checksum="C3E3BF54C4D588D0" modified="2016-10-05" version="1">MSSKQRQVHICLSGHTANICDKLPSNLGRLSLIDSLISAFGLYSKCLLVNVVPASAKELCVFHDEKYVAELLKERHEENTSFSKEEAKYGLLFDCPPFAGLHEYVLHVTGSSLSAANVLLRNRDTDSQHVVINWYGGRHHAHKAGASGFCYVNDIVLAILRLRTLYRKIFYLDVDLHHGDGVEAAFRFLKFVTTCSVHRHDLGFFPGTGNSSGAGAYNIPTKRGLGDKNFKDIIARVVAPIIAKHTPDIIVVQCGSDGLASDPSGEWNLSIDGLASSISGLVDKFQSVPFLVLGGGGYNHTETARFSAWLTKTLIGDVTEWGDIPEHACLDAYENDGFQFWTPGNKAGKPARCDENKDIGAIRANTLEYLWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFS9</accession>
    <name>A0A1A0HFS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_29559</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713495.1">
        <property type="nucleotide sequence ID" value="XM_018855437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFS9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFS9"/>
    <dbReference type="GeneID" id="30028413"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="61"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="713" mass="79685" checksum="8088C2499205FCF0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGC0</accession>
    <name>A0A1A0HGC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoporin Nup120/160 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713408.1">
        <property type="nucleotide sequence ID" value="XM_018856817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGC0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGC0"/>
    <dbReference type="GeneID" id="30029793"/>
    <dbReference type="OrthoDB" id="67716at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059141">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11715">
        <property type="entry name" value="Beta-prop_Nup120_160"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleoporin Nup120/160 beta-propeller" evidence="1">
        <location>
            <begin position="84"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1153" mass="130857" checksum="69BB275AD6ADD2FB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGC5</accession>
    <name>A0A1A0HGC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_29496</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NHP6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713413.1">
        <property type="nucleotide sequence ID" value="XM_018855418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGC5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGC5"/>
    <dbReference type="GeneID" id="30028394"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01390">
        <property type="entry name" value="HMG-box_NHP6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000016">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112:SF22">
        <property type="entry name" value="MITOCHONDRIAL TRANSCRIPTION FACTOR A, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00886">
        <property type="entry name" value="HIGHMOBLTY12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HMG box" evidence="10">
        <location>
            <begin position="18"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="8">
        <location>
            <begin position="18"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="89" mass="10344" checksum="9EFA7DDB8FA7CBEF" modified="2016-10-05" version="1" fragment="single">MSTEKKKSTRKKKDPDAPKRSLSAYMFFANENRDIVRAENPGITFGQVGKMLGERWKALNADEKVPYENKAATDKKRYEKEKAEYAKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGK0</accession>
    <name>A0A1A0HGK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713601.1">
        <property type="nucleotide sequence ID" value="XM_018859385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGK0"/>
    <dbReference type="GeneID" id="30032360"/>
    <dbReference type="OrthoDB" id="8300214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="37"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="146" mass="15934" checksum="1C5693EEA988B32B" modified="2016-10-05" version="1">MSLPSVPDWLAKWGSEQLETKPHRMSKDELLAILKSAPETVTVVDLRNDRERGYITKAVHIPATVIEGPTDIQNKLVGPVLAQFPQTKKIVVHCNLSAKRAGLIGAWGKDHVAANGPQDVDVQILHEGIVGWLAAGDDFKDETTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGM2</accession>
    <name>A0A1A0HGM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative calmodulin-like myosin light chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_9594</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713800.1">
        <property type="nucleotide sequence ID" value="XM_018859413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGM2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGM2"/>
    <dbReference type="GeneID" id="30032388"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071341">
        <property type="term" value="C:medial cortical node"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000356">
        <property type="entry name" value="Myosin II light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000082">
        <property type="entry name" value="Myosin light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0518">Myosin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="74"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="142" mass="15889" checksum="F4D65AFE517AEA5A" modified="2016-10-05" version="1">MSNTKTYKDAFALFDKKGTGKIPIEHLGDLLRAVGQNPTLAEIAELQESVKLSDFDFETYQNIINRPDGFKPLGLPEDYIKGFQVFDKDHTGYIGVGELRYILTSIGEKLNDLEVDELLKGVNVTADGNVDYVEFVKSILDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH10</accession>
    <name>A0A1A0HH10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="14">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_29924</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX55/SPB4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="14"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713945.1">
        <property type="nucleotide sequence ID" value="XM_018855514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH10"/>
    <dbReference type="STRING" id="869754.A0A1A0HH10"/>
    <dbReference type="GeneID" id="30028490"/>
    <dbReference type="OrthoDB" id="7396459at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056330">
        <property type="entry name" value="CTT_SPB4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23681">
        <property type="entry name" value="CTT_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 13 19" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="37"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="256"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="546"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA23464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="607" mass="68992" checksum="F100CB8A2E4C121A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH12</accession>
    <name>A0A1A0HH12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_99485</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713619.1">
        <property type="nucleotide sequence ID" value="XM_018859496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH12"/>
    <dbReference type="GeneID" id="30032471"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="200" mass="22891" checksum="E26347E8C8CD8140" modified="2016-10-05" version="1">MKGSADGSLARSPGLRQQHQQHSPSKGQRVVNSRTPREIRAGKTKRKANKTKEQQQEQQEQQEQQEQQEKKEKQEQQEQQEQQEQQTNKQASAFSAGLRRPGSILFHHGPSADPLEGAARPLSQRENRPQPRPTSQHPDHAAPSPSRPKPRPRQDQNQIPNTDNQTRIEDQNRPRPKQTETKQAQKMKLNTTKPARQNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHA8</accession>
    <name>A0A1A0HHA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_36251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat mio family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713867.1">
        <property type="nucleotide sequence ID" value="XM_018856711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHA8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHA8"/>
    <dbReference type="GeneID" id="30029687"/>
    <dbReference type="OrthoDB" id="341486at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16691">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21720">
        <property type="entry name" value="MIOS_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17034">
        <property type="entry name" value="zinc_ribbon_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="5">
        <location>
            <begin position="465"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="GATOR2 complex protein MIO zinc-ribbon like" evidence="4">
        <location>
            <begin position="832"/>
            <end position="958"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="958" mass="107359" checksum="FD262402CB1E9CC6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHF1</accession>
    <name>A0A1A0HHF1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_105395</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713912.1">
        <property type="nucleotide sequence ID" value="XM_018853794.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HHF1"/>
    <dbReference type="GeneID" id="30026770"/>
    <dbReference type="OrthoDB" id="29024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="8">
        <location>
            <begin position="619"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="7">
        <location>
            <begin position="895"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1245"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="917"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1245"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1318"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1351"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1372"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1396"/>
            <end position="1418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1419"/>
            <end position="1438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1448"/>
            <end position="1457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1457" mass="165859" checksum="9EBE8FA912A7968A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHP6</accession>
    <name>A0A1A0HHP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_36812</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714011.1">
        <property type="nucleotide sequence ID" value="XM_018856779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHP6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHP6"/>
    <dbReference type="GeneID" id="30029755"/>
    <dbReference type="OrthoDB" id="565731at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF9">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="4">
        <location>
            <begin position="63"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="275" mass="31583" checksum="270022692BE34BF1" modified="2016-10-05" version="1">MSNFKLRDVIHSYKYALNDEEKTVKPKTRSKVAKPSINRKKIDSKITDRYTKQDHDLKDLPPSVKPGLTLVFVGFNPGVESSKKQHHYAHPTNLFWKLFNALDILSVVLDIRNVSVLAHGKLISEIYCDGKSQARAEHDFKLLDLGIGFTDLVLRCTKQASELSLHEKAENVPRLLKEFEISQAPFIVFIGKGIWEVVCKYLVPKHRLNAHNFSWGLQKDLLVMLRFNTFCLHRPEVYVFPNTSGLVALMKYPQKLDLWQNLVADMRKIPDSENG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHU6</accession>
    <name>A0A1A0HHU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Origin recognition complex, subunit 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_36327</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714057.1">
        <property type="nucleotide sequence ID" value="XM_018856723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHU6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHU6"/>
    <dbReference type="GeneID" id="30029699"/>
    <dbReference type="OrthoDB" id="5367324at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008721">
        <property type="entry name" value="ORC6_cyclin_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016811">
        <property type="entry name" value="ORC6_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05460">
        <property type="entry name" value="ORC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF022941">
        <property type="entry name" value="ORC6_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ORC6 first cyclin-like" evidence="7">
        <location>
            <begin position="10"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="380" mass="43590" checksum="92D35B0186194E0B" modified="2016-10-05" version="1">MNRSHIQQSLKDIVPNFAGAYPPALLSYIDSVYQLSLQKLPILPHKADVARYHLCTYLTVERYQQKFSLPTPNLQKVPVQPKFLEKLLDDIQDKVMSSMSTPASTPKKLFADEMLPSKKRPFTPTVGSPLRKLQKLLGIQDEPPQKAVESPFNTPLKSLATSPTRDIFKTPRTSPAKNPQTCSPSSSRYLKQLSIPDFVSFANNFYIPASVTPHLLEMFVAERYKFVRRNEWLLACGLIHAAYVRINDKLFKNTIGKKSEFQDQLFQYQKGNLMKKVMIEWINRVEESVKGQPWALDLELKYVHNDWTVEDTTREKEIEAKLGKGWEIVRGLGSFIDPSVMFDKPSQIEYYNTWARRLREKVKSLGTPPPETSQDLPLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHY5</accession>
    <name>A0A1A0HHY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_9751</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713973.1">
        <property type="nucleotide sequence ID" value="XM_018859455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHY5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHY5"/>
    <dbReference type="GeneID" id="30032430"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="220" mass="24801" checksum="ACFB31F9E6107552" modified="2016-10-05" version="1">MYFGIHGSDLLVYSFNIFSEQQWINSGTMVFQGVYGRKVPVKIKGNSENRPLITNLGKICLNNAMWRTHMGIEGIGCINVGPYSRLNFVFDESQIRNRQTIVLDSYSELRISKLDLASSVPYIKVVGLGESNGVTVDVRIESDKIDYSKDTGLLTLKKSGQDMIRLRIGRGYDENRFNVTYNYFGSTLVYKHAAPTLSYEICSCDSKFPDTPKVPAYESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI72</accession>
    <name>A0A1A0HI72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76571</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714063.1">
        <property type="nucleotide sequence ID" value="XM_018858554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI72"/>
    <dbReference type="STRING" id="869754.A0A1A0HI72"/>
    <dbReference type="GeneID" id="30031530"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF55">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444440" description="Transmembrane 9 superfamily member" evidence="9">
        <location>
            <begin position="17"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="399"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="431"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="496"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="531"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="566"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="599"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="638" mass="71470" checksum="5E7B62092E88B9D0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI84</accession>
    <name>A0A1A0HI84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_35864</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714360.1">
        <property type="nucleotide sequence ID" value="XM_018856662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI84"/>
    <dbReference type="STRING" id="869754.A0A1A0HI84"/>
    <dbReference type="GeneID" id="30029638"/>
    <dbReference type="OrthoDB" id="5428495at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF17">
        <property type="entry name" value="VITAMIN B6 TRANSPORTER TPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="533" mass="58945" checksum="3E9AAC6A580F3B86" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HID5</accession>
    <name>A0A1A0HID5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_112376</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714242.1">
        <property type="nucleotide sequence ID" value="XM_018853940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HID5"/>
    <dbReference type="GeneID" id="30026916"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="91" mass="9862" checksum="3359E1F0D520AF31" modified="2016-10-05" version="1">MGLVSWDWCHGIGVMGLVSWDWCHGIDLTRCRRISWCHGIGVIASGGVRNLLCFWDPCFTNPDATSSLNGSASSLSYNVLGLLLGNHQTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIW8</accession>
    <name>A0A1A0HIW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Iron transport multicopper oxidase FET3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_37244</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the multicopper oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714264.1">
        <property type="nucleotide sequence ID" value="XM_018856846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIW8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIW8"/>
    <dbReference type="GeneID" id="30029822"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901684">
        <property type="term" value="P:arsenate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033215">
        <property type="term" value="P:reductive iron assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13851">
        <property type="entry name" value="CuRO_1_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13877">
        <property type="entry name" value="CuRO_2_Fet3p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13899">
        <property type="entry name" value="CuRO_3_Fet3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000022">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000024">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000025">
        <property type="entry name" value="FET5p Multicopper oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044130">
        <property type="entry name" value="CuRO_2_Fet3-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF361">
        <property type="entry name" value="IRON TRANSPORT MULTICOPPER OXIDASE FET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0186">Copper</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="16 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 19" id="KW-0732">Signal</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291820" description="Iron transport multicopper oxidase FET3" evidence="19">
        <location>
            <begin position="19"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="557"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="22">
        <location>
            <begin position="30"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="20">
        <location>
            <begin position="155"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="21">
        <location>
            <begin position="366"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA23783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="25">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="624" mass="70367" checksum="1316311FA2B23FDC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIZ6</accession>
    <name>A0A1A0HIZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">F1F0-ATPase complex, subunit H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_76792</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714338.1">
        <property type="nucleotide sequence ID" value="XM_018858587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIZ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIZ6"/>
    <dbReference type="GeneID" id="30031563"/>
    <dbReference type="OrthoDB" id="274752at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019711">
        <property type="entry name" value="ATP_synth_F0_suH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28207">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT H, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28207:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT H, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10775">
        <property type="entry name" value="ATP_sub_h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="105" mass="11475" checksum="B57C3157FCBE43FE" modified="2016-10-05" version="1">MYRPRALSSRRLFSVTPQRSNLVSDLYVSQIKQFKPTQTSAEGAEGIKKFQLPSKPAVPSEEVSADAVSAYESAAVETTSAPSTEAAPAAEEDWFVFEEAEAEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIZ9</accession>
    <name>A0A1A0HIZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_30346</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714471.1">
        <property type="nucleotide sequence ID" value="XM_018855621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIZ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIZ9"/>
    <dbReference type="GeneID" id="30028597"/>
    <dbReference type="OrthoDB" id="1158011at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF3">
        <property type="entry name" value="HOLOCYTOCHROME-C SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="263" mass="29687" checksum="FD245E255BF9E967" modified="2016-10-05" version="1">MGWFWASPAASTNAVAGRPPAVCPVDHKNMPGAACPVQKPGREADDIEVLNPLNNMPMAISSERAPGQKVVLSTERTISLIPRGVLEDQGNWEYPSPQQMLNAMLKKGKGQGIDETAVESMVDVHNFLNEGAWLQILDWELKHTLQSKVEPRLEKFMGRPHDLSPRARMYLYLGRLFPETFNTQPPFDRHDWTVLRSAGHGRGWLKVRYVIDYYGAPDDEETGMPAFMLDTRPALDSPTAAADRLDRWFTPLWKRAMGEFDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ22</accession>
    <name>A0A1A0HJ22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30227</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714319.1">
        <property type="nucleotide sequence ID" value="XM_018855579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ22"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ22"/>
    <dbReference type="GeneID" id="30028555"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15489">
        <property type="entry name" value="PHD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052788">
        <property type="entry name" value="RING-type_E3_ligase_ATL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798:SF97">
        <property type="entry name" value="ALCOHOL-SENSITIVE RING FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45798">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL61-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="3"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="170"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="384"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="455"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="396"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="613" mass="64744" checksum="5031C20BD3D7A111" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ27</accession>
    <name>A0A1A0HJ27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_113968</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714324.1">
        <property type="nucleotide sequence ID" value="XM_018853975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ27"/>
    <dbReference type="GeneID" id="30026951"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="28328" checksum="AF8258EC82AC3DE4" modified="2016-10-05" version="1">MHASHVPCVRVTCQRSRHGARVRITRAPFPCLATWQLTASRRRKTCQKIICRLGARCAYVLHRQPELRCFTAETAPAASSSFCFSFSFFQLAVILSRGPENVSVLEAIPRYKRKAFGGSGMRAGFLQDGAAENEHFQAGPPLLQQNDIRGYAPDCTARPVLADSAELHLRKTAFQNGRGPPHLGSSFLFFLFFLFFLLSSSFLFSSFLHPSIPGAGPGHLISRTPYPVSGRSMPAHPTLQISETSPDLSVRRSLPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ32</accession>
    <name>A0A1A0HJ32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 21">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="15">IMP dehydrogenase</shortName>
            <shortName evidence="15">IMPD</shortName>
            <shortName evidence="15">IMPDH</shortName>
            <ecNumber evidence="15 21">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_76784</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15 21">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="15">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15 21">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer. Seems to be able to form heterotetramers composed from more than 1 of the 3 IMPDH gene products (IMD2-4).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15 20">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="15 21"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714329.1">
        <property type="nucleotide sequence ID" value="XM_018858585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ32"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ32"/>
    <dbReference type="GeneID" id="30031561"/>
    <dbReference type="OrthoDB" id="416622at2759"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000007">
        <property type="entry name" value="Chromosome 19 SCAF14664, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 19" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 21" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="5 15 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 15 17" id="KW-0520">NAD</keyword>
    <keyword evidence="9 15 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 15 18" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 15 21" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="119"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="22">
        <location>
            <begin position="182"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="15 16">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15 16">
        <location>
            <position position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="276"/>
            <end position="278"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15 17">
        <location>
            <begin position="326"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15 18">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="366"/>
            <end position="368"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="389"/>
            <end position="390"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="413"/>
            <end position="417"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA23848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="521" mass="55512" checksum="5CAA1880EE4C457A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX5</accession>
    <name>A0A1A0HJX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714960.1">
        <property type="nucleotide sequence ID" value="XM_018856543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX5"/>
    <dbReference type="GeneID" id="30029519"/>
    <dbReference type="OrthoDB" id="4087488at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="368" mass="41597" checksum="BA0E7607611E1C5F" modified="2016-10-05" version="1">MCIEKPSKTQITAGRLSGPAASENPNLHPPGSQPLLQPGQIPPLRRKKPSGADPFIELYVKRDCSELSLDVAQTQPFWKFHVLKFGHCLYLTTNPTLRHLHCRNAPGYFVQVCDGPCGFSMIFEDISTGEELVKIDKLTDRCSNQFMFHVRKNRHVKDGRFQKSTRNGNAESRALEPIPQELLPATPTTPMLSYSSQMIDGLLWSVGSLPQYRESRIKSQEIKYIAKKNVYFHRCFGDISRYPEYDVPPAMAVFRECESGSRKRAMRQLNRFLQAGHELSSKLLKLPPANVSPYAEITLYYKGGDGVYFDKVPADDEPDHHHKLGWVTVYDDPHVFSKQGMFDLVVALTTAIGYERNLAFEASNSNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK02</accession>
    <name>A0A1A0HK02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_118398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714619.1">
        <property type="nucleotide sequence ID" value="XM_018854109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK02"/>
    <dbReference type="GeneID" id="30027085"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="142" mass="16545" checksum="4FCE4CF6A564B392" modified="2016-10-05" version="1">MRIRSVDYGTEQTHLCNRNSPKLLNIGVGWEMQRAHSYITASYATPRLGVSRTINWRDKKIGVRIRFLFFFFFSSRVYKEPWCFRYTEMTAELTCLVSGVPKFGALFVSAMCEFVCWLLLFHLVGFRIGDGRRRAGDKTARI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK42</accession>
    <name>A0A1A0HK42_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leo1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715030.1">
        <property type="nucleotide sequence ID" value="XM_018853827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK42"/>
    <dbReference type="STRING" id="869754.A0A1A0HK42"/>
    <dbReference type="GeneID" id="30026803"/>
    <dbReference type="OrthoDB" id="20844at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007149">
        <property type="entry name" value="Leo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146">
        <property type="entry name" value="LEO1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23146:SF0">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN LEO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04004">
        <property type="entry name" value="Leo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="390" mass="45158" checksum="17E13E96A0FB0997" modified="2016-10-05" version="1">MSDTEISNRGGKEEDVEDLFGDDEDEDQTTSKIASQSQDENESEEDDRRVNNESDQDSQDEEEESEKQLKVADITIPRHAKSYKPEEDTYSIKMPVYLNVEAHPFDPNEFKEKVQQSAIERSKSSLDSAAMRSASISEKLLNENTIRWRYSNSGNDEIIKQSNAHFIQWDDGLLSLKIGKEIFDFRDLPLMDNFLARSHVEHEILQNDSVLTKSASLLPSSTATNTHRKLTQAVKNVQKKDKILNTLTNDDPMMKQRKADEDERKTLKMKRQLEQRRRLQEERLGKVDSPAPSRGSYSSAYERFERTYGDDEYDDEDDFVANDDEEPEMDEDEEEDGEGFDANDEEEAFDKGAERLKSLKSEGQFKYKSSTPDDNEDRRKRRRIIDSDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKC6</accession>
    <name>A0A1A0HKC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome-recycling factor, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ribosome-releasing factor, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_38400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Necessary for protein synthesis in mitochondria. Functions as a ribosome recycling factor in mitochondria.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714937.1">
        <property type="nucleotide sequence ID" value="XM_018856998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKC6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKC6"/>
    <dbReference type="GeneID" id="30029974"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="entry name" value="Ribosome-recycling factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982:SF3">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME RECYCLING FACTOR PSEUDO 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="RIBOSOME RECYCLING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="Ribosome recycling factor, RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome recycling factor" evidence="6">
        <location>
            <begin position="76"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="27478" checksum="FD52327F5FAA21B8" modified="2016-10-05" version="1">MLRSILLKQASANTSRVFLASNLKPCRNIHWSPLLFKKRGKEPVQKDEPTESAKSPEIDFDEVSQRFQGVLDKFAKHANESKLGKTNPQIFDNLQVETPDGELPYTAVAITALKGRNFIMTVFDPANVKHVVNAVLASDLNMNPVLDPSNKQMLKVPLPPVTTESKKESIKQLKAVFDKFKNGAGGSGKHAHTLSAIRADIKNKISTKKKMNDQEQALWKKVESVHKEYVKKLEDSFKAAETAIMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKE7</accession>
    <name>A0A1A0HKE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA N-acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_134471</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714976.1">
        <property type="nucleotide sequence ID" value="XM_018854364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKE7"/>
    <dbReference type="STRING" id="869754.A0A1A0HKE7"/>
    <dbReference type="GeneID" id="30027340"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000065">
        <property type="entry name" value="N-terminal acetyltransferase complex ARD1 subunit homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="193" mass="22678" checksum="FFB7F6D2A5532E67" modified="2016-10-05" version="1">MTSIKPFKMEDLYELNSVNLDPFTENFNLLFYLQYLCDWPTLFFKSVEDTGNAKREISGYMMGKTEGQLVMKEWHTHITAVTIGRNFRRIKLASDLCTHLEQITAIAPHEALFVDLFVKVTNTVARQLYEKLGYLVYRRVIGYYGDKYPTDRDHVNDDVDAFDMRKALPRDVKRETVRENGEKFNVFPHEVTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKF8</accession>
    <name>A0A1A0HKF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_10520</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714858.1">
        <property type="nucleotide sequence ID" value="XM_018853789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKF8"/>
    <dbReference type="STRING" id="869754.A0A1A0HKF8"/>
    <dbReference type="GeneID" id="30026765"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000020">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000046">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 14" id="KW-0647">Proteasome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="188"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="411" mass="45926" checksum="8AE7D2B85071C1D9" modified="2016-10-05" version="1">MEELGILPPVAPAMSFFKPKESAPQEETDIYLKLKKLEKELDLLLLQEDYIKDELRHLKRELVRAQEEVKRIRSVPLVIGQFLEPIDENTGIVSSTTGSNYAVRILSTLDRELLKPSSSVALHRHSNALVDILPPEADSSIAIVGDNQKPDVTYADVGGLDMQKQEIREAVELPLTQGDLYSQIGIDPPRGVLLYGPPGTGKTMLVKAVANSTTASFIRINGSEFVQKYLGEGPRMVRDVFRLARENSPSIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQSSNVKVIMATNRADTLDPALLRPGRLDRKIEFPNLKDRRERRLIFSTIASKMALAPEADLDSLIVRNDPLSGAVIAAIMQEAGLRAVRKNRYMILQSDLEEAYAAQVKTGSEHDKFDFYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKI4</accession>
    <name>A0A1A0HKI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_77139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714799.1">
        <property type="nucleotide sequence ID" value="XM_018858651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKI4"/>
    <dbReference type="GeneID" id="30031627"/>
    <dbReference type="OrthoDB" id="4091783at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="406" mass="46446" checksum="50F81B485EDC7D4B" modified="2016-10-05" version="1">MKTPKLNTRPLRLLKKWGNSPTAWIRHNLFLLGYEKVRFYAYQKLSPLAIQKGGGFIKCVVADLNSFVKREWASEGELFLKICSSVHFFEDANIPDLRLFFTSVGSMMLSGKEKNVRIITDELLELYIEKLRGKISLDTPASKSERQILKISTNYYQTCKINYRTLYDPKIGPRSITIGDRTFSIKLPPLPQISSKQLLARALLHKEMYPALMLPGHTVHDQLSRSQILMAPATAKVLRYDLLFLDGLGDLLIASESCQILYQFRLLHPYSSDTSFGKKTFTLLKNILGTNSLLLRLATAYNMHLALEDPTVSTLLRESYIPQTFAKATADTDIGQYEEEFLADYFEQYSGALFLDLPEAAKEWLSELFERILALISDTYRINHRRDANALFDYRAWSVDVIGRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKL5</accession>
    <name>A0A1A0HKL5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_30644</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
            <location evidence="1 5">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the NRAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714829.1">
        <property type="nucleotide sequence ID" value="XM_018855676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKL5"/>
    <dbReference type="STRING" id="869754.A0A1A0HKL5"/>
    <dbReference type="GeneID" id="30028652"/>
    <dbReference type="OrthoDB" id="10251401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005554">
        <property type="entry name" value="NOL6/Upt22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035082">
        <property type="entry name" value="Nrap_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035367">
        <property type="entry name" value="Nrap_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035368">
        <property type="entry name" value="Nrap_D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035369">
        <property type="entry name" value="Nrap_D4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035370">
        <property type="entry name" value="Nrap_D5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035371">
        <property type="entry name" value="Nrap_D6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17972">
        <property type="entry name" value="NUCLEOLAR RNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03813">
        <property type="entry name" value="Nrap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17403">
        <property type="entry name" value="Nrap_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17404">
        <property type="entry name" value="Nrap_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17405">
        <property type="entry name" value="Nrap_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17406">
        <property type="entry name" value="Nrap_D5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17407">
        <property type="entry name" value="Nrap_D6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nrap protein" evidence="7">
        <location>
            <begin position="200"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="8">
        <location>
            <begin position="349"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="9">
        <location>
            <begin position="504"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="10">
        <location>
            <begin position="686"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="11">
        <location>
            <begin position="872"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="Nrap protein" evidence="12">
        <location>
            <begin position="1044"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1180" mass="131290" checksum="B04A7EF22358D40A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GNY6</accession>
    <name>A0A1A0GNY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transmembrane type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_48213</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000043">
        <property type="protein sequence ID" value="OBA13454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709216.1">
        <property type="nucleotide sequence ID" value="XM_018858278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GNY6"/>
    <dbReference type="STRING" id="869754.A0A1A0GNY6"/>
    <dbReference type="GeneID" id="30031254"/>
    <dbReference type="OrthoDB" id="4090645at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008559">
        <property type="term" value="F:ABC-type xenobiotic transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18597">
        <property type="entry name" value="ABC_6TM_YOR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF456">
        <property type="entry name" value="MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN LETHAL(2)03659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="141"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA13454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="407" mass="46047" checksum="285F4E42DD0A756F" modified="2016-10-05" version="1" fragment="single">MSDLQLQKRLLTPFLSKNIPPIPEENERREYPKKVNPLSYLFFWWLHPVMKVGYKRTLTPADMYTLNDNIKVETLTRKFQEIFRTRLEKAQHAHVLAKVTLRGETMETSSIPYADDVRDLELSKHFLTISLFLTFKWQYSLACTFLVLASVGSTTAPLLTKELIKFVEYRVLDIDVNMGSGVGYALGSSFLVLVVGLLMNHTFQNAMLTGAQAKAVLTKSILDKSFKLNGQLKHDYPVSKITSMMGTDLARIDFAMGFQPFLVSFPVAVGIAIGILCHNIGASAMVGIGLTFVFIVLMGLVTKKLFAYRASANKYTDTRVNYMKEVLSNLKMIKFYSWENPYLDRITENRTKEMHIIYNMQMIRNMIVSVAMSLTLFASMASFLVVYATLGSTKSPADIFSSVSLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1S1</accession>
    <name>A0A1A0H1S1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ARM repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_13892</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709371.1">
        <property type="nucleotide sequence ID" value="XM_018854443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1S1"/>
    <dbReference type="STRING" id="869754.A0A1A0H1S1"/>
    <dbReference type="GeneID" id="30027419"/>
    <dbReference type="OrthoDB" id="361693at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="5">
        <location>
            <begin position="27"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA17976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="989" mass="113510" checksum="CB43870054983543" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1S2</accession>
    <name>A0A1A0H1S2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_84919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709374.1">
        <property type="nucleotide sequence ID" value="XM_018859242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1S2"/>
    <dbReference type="STRING" id="869754.A0A1A0H1S2"/>
    <dbReference type="GeneID" id="30032217"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="31"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="418" mass="48798" checksum="2CC8C58BC4D9D95A" modified="2016-10-05" version="1">MDQEQLRIVKPAVSPKRNCRENPERKMESSVVCLEKLPLQILEMIFDLLETKYLLDIRTISRDLYWPATRVLYRFIIMVDEVDFDDIRVQESIWPYYHCTIMNVRQTVILVDVLKENKKLAAMVESIVVGNTDEVSLVPELLSYTSLKYFHYEGILQIPRKSLDNVRTLACCVPSASIEKPNVTELKLYQPLIGTVPDFYAHASSSMTSLGSYRKLRKLSIAVTHWSNDSYFELEEMNTHSWEYFISNFMRYHELQLDSLRIEGQFLDFYDSRRSFDLYQGVNLGILKTLELKFVGYCPSGEQYVDHHSTEFSYDLSKYLHLLKNLSLIFEYTVQTNVESDIKTLAKVLDGKLRNQLSLFSISFEPHARASLCYLHNAILRSQKQLVNLKINNGDIFSRIGRTRFDKDPNLKRKKTMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Y6</accession>
    <name>A0A1A0H1Y6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TEA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_227294</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709439.1">
        <property type="nucleotide sequence ID" value="XM_018855189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Y6"/>
    <dbReference type="GeneID" id="30028165"/>
    <dbReference type="OrthoDB" id="10006572at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TEA" evidence="3">
        <location>
            <begin position="241"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="807"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="840"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA18044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="873" mass="94959" checksum="72F677931E2DF26C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V5</accession>
    <name>A0A1A0H4V5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33609</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709515.1">
        <property type="nucleotide sequence ID" value="XM_018856350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V5"/>
    <dbReference type="STRING" id="869754.A0A1A0H4V5"/>
    <dbReference type="GeneID" id="30029326"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000008">
        <property type="entry name" value="Proteasome subunit beta type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA18980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="195" mass="21769" checksum="55FB07CECD7066F2" modified="2016-10-05" version="1">MDIVLGIKVQDATILATSKAATRGISVLKATDDKTRLLNTHNLVAYTGEAGDTVQFAEYIQANVQLYTMRENGQELSPRATASFVRNQLATSIRSRKPYQVNVLIGGFDTKTNTPSLNWIDYLGTQVELPYGAHGYAAFYCTSLFDRHYRPDMTLEEGIDLLKRSLAELETRMPIDFKGVYVKVVDKDGARPLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H525</accession>
    <name>A0A1A0H525_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_47118</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709673.1">
        <property type="nucleotide sequence ID" value="XM_018858127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H525"/>
    <dbReference type="STRING" id="869754.A0A1A0H525"/>
    <dbReference type="GeneID" id="30031103"/>
    <dbReference type="OrthoDB" id="18781at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17923">
        <property type="entry name" value="DEXHc_Hrq1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18797">
        <property type="entry name" value="SF2_C_Hrq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055227">
        <property type="entry name" value="HRQ1_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47957:SF3">
        <property type="entry name" value="ATP-DEPENDENT HELICASE HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="MZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22982">
        <property type="entry name" value="WHD_HRQ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="335"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="603"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1142"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1152"/>
            <end position="1162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1195" mass="134009" checksum="36BD26E978B0F28B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H529</accession>
    <name>A0A1A0H529_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_224787</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709590.1">
        <property type="nucleotide sequence ID" value="XM_018855167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H529"/>
    <dbReference type="STRING" id="869754.A0A1A0H529"/>
    <dbReference type="GeneID" id="30028143"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="109"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="380" mass="43875" checksum="39C9534335BB7C8A" modified="2016-10-05" version="1">MKQAVLLRRSKLVVPLIVLGAATYIDYASCYIVAWKEVYHRRLSASAIVFWVLLAIFHVLCLFYWALIFIRGPGHIPKFQNFNLYQSQEKGTAPVPDVFVCDEQGFPFWCSKCQNLKPDRSFHLTDLNTCVPRFDHFCLWIGTAIGRDNFIPFIKFLQSLLAYVTIVLCFAAVYTRSSLRHDHSNLPHFIVLFVLSAFGTIFTLSLLIGTVKYTFTNSTSFDEITRRQAMTYRRWLGKQRNVNSRYTWCVGKTPRVELGIRFVNIAHEGTRKVVSYTFDDNPYSNGFKVNLINMCLNKNYNVNLTRDIAGLLRLMKSSLVAIVPFAEFFIATSARDKTLASFQQNSDEFSPEFMQKMLDKIEKKEFKHALYVAPIIHDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H579</accession>
    <name>A0A1A0H579_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_219964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709723.1">
        <property type="nucleotide sequence ID" value="XM_018855127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H579"/>
    <dbReference type="GeneID" id="30028103"/>
    <dbReference type="OrthoDB" id="4026805at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="655" mass="70611" checksum="7A5D4B35561A7602" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B3</accession>
    <name>A0A1A0H5B3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_46747</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709672.1">
        <property type="nucleotide sequence ID" value="XM_018858071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5B3"/>
    <dbReference type="GeneID" id="30031047"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="15"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="621"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="646"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="686" mass="77191" checksum="A93610895A2F9E62" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F3</accession>
    <name>A0A1A0H5F3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_47133</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709851.1">
        <property type="nucleotide sequence ID" value="XM_018858129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F3"/>
    <dbReference type="GeneID" id="30031105"/>
    <dbReference type="OrthoDB" id="6129702at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140278">
        <property type="term" value="P:mitotic division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052557">
        <property type="entry name" value="CAP/Cytokinesis_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056409">
        <property type="entry name" value="Ig_CYK3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46333:SF2">
        <property type="entry name" value="CYTOKINESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24584">
        <property type="entry name" value="Ig_CYK3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="18"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="890" mass="101500" checksum="438FF659D70373DF" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5G3</accession>
    <name>A0A1A0H5G3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33512</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the OST3/OST6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709861.1">
        <property type="nucleotide sequence ID" value="XM_018856316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5G3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5G3"/>
    <dbReference type="GeneID" id="30029292"/>
    <dbReference type="OrthoDB" id="67566at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF0">
        <property type="entry name" value="GH11935P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291668" evidence="10">
        <location>
            <begin position="20"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="38123" checksum="947904E0175570F0" modified="2016-10-05" version="1">MKCWTPLFLVSLLWASVLATYTNDELHNVLESKGKNGIIHLSDENYQKVLYGERDYHLILFLTSRNPKISCFLCGEFAPYYETVANSYRRTYPKGITEDGKNVYFLVSELDDSKKLFKLLALQSIPKVYHFPPTPKDTKPSTFLSDSHEYEFYQGDHIELLSKWVTAVTGNSIDLYIPVDYSKIATHALITFGLIFALKMYSTRLISIVRSPLLWGLVSIVLILMFLTGHMFNQMKNSPYVRDDGRKVEYFAPSPQMQYIFETQALSSLYGLSGLLFLVLTVRVSKIKDAKAQLLASVVVCACLYLLYGIFLNIFGIKYGGYPYRFFEFLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5X4</accession>
    <name>A0A1A0H5X4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ARM repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_46449</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709839.1">
        <property type="nucleotide sequence ID" value="XM_018858042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5X4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5X4"/>
    <dbReference type="GeneID" id="30031018"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="38"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="712"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="713"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="743"/>
            <end position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="760" mass="84661" checksum="1BCB6EB56975C96D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Z9</accession>
    <name>A0A1A0H5Z9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_46707</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709864.1">
        <property type="nucleotide sequence ID" value="XM_018858066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Z9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Z9"/>
    <dbReference type="GeneID" id="30031042"/>
    <dbReference type="OrthoDB" id="1746530at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="158" mass="17936" checksum="5BD83EEC0034FA19" modified="2016-10-05" version="1">MKVLNEREAFLLDYEILQHLTGIKEKFSWSFTPEDDANDKYRRKRFAGAGQGLEAITRDILLYLAKNAAGSVTSEASFGELVTFLNAFDLMKAEKLQIVNSLPRSMVHLYGLVEECDLRFDEDTCQSIIDKIDELFPLAQEEGEEEENEAEDDAMADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H608</accession>
    <name>A0A1A0H608_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709994.1">
        <property type="nucleotide sequence ID" value="XM_018857992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H608"/>
    <dbReference type="STRING" id="869754.A0A1A0H608"/>
    <dbReference type="GeneID" id="30030968"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="496"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="500"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="666" mass="76641" checksum="AB2C2FA063349960" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H615</accession>
    <name>A0A1A0H615_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylserine decarboxylase proenzyme 2</fullName>
            <ecNumber evidence="11">4.1.1.65</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="11">Phosphatidylserine decarboxylase 2 beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="11">Phosphatidylserine decarboxylase 2 alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="11" type="primary">PSD2</name>
        <name evidence="14" type="ORF">METBIDRAFT_33534</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine + H(+) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:20828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="4.1.1.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="11">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit. Interacts with pstB2. This interaction may be a means to structurally tether the donor membrane (ER) harboring PstB2 to acceptor membranes (Golgi/endosomes) harboring PSD2 during PtdSer transport to the site of PtdEtn synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Golgi apparatus membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endosome membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The C2 domains have an essential, but non-catalytic function. They may facilitate interactions with other proteins and are required for lipid transport function.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The autoendoproteolytic cleavage occurs by a canonical serine protease mechanism, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain. During this reaction, the Ser that is part of the protease active site of the proenzyme becomes the pyruvoyl prosthetic group, which constitutes an essential element of the active site of the mature decarboxylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the phosphatidylserine decarboxylase family. PSD-B subfamily. Eukaryotic type II sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.65" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709890.1">
        <property type="nucleotide sequence ID" value="XM_018856322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H615"/>
    <dbReference type="STRING" id="869754.A0A1A0H615"/>
    <dbReference type="GeneID" id="30029298"/>
    <dbReference type="OrthoDB" id="67700at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00616"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016540">
        <property type="term" value="P:protein autoprocessing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04039">
        <property type="entry name" value="C2_PSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00663">
        <property type="entry name" value="PS_decarb_PSD_B_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033177">
        <property type="entry name" value="PSD-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033179">
        <property type="entry name" value="PSD_type2_pro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00163">
        <property type="entry name" value="PS_decarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF17">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10 11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023273698" description="Phosphatidylserine decarboxylase 2 beta chain" evidence="11">
        <location>
            <begin position="1"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023273697" description="Phosphatidylserine decarboxylase 2 alpha chain" evidence="11">
        <location>
            <begin position="995"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="338"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="146"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="846"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="908"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system; for autoendoproteolytic cleavage activity" evidence="11">
        <location>
            <position position="995"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid; for decarboxylase activity" evidence="11">
        <location>
            <position position="995"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="11">
        <location>
            <begin position="994"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="11">
        <location>
            <position position="995"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1109" mass="125989" checksum="158D24131937F15D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H679</accession>
    <name>A0A1A0H679_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_214330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709993.1">
        <property type="nucleotide sequence ID" value="XM_018855075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H679"/>
    <dbReference type="GeneID" id="30028051"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291719" evidence="1">
        <location>
            <begin position="20"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="94" mass="9805" checksum="2C959EB76EB4D1AE" modified="2016-10-05" version="1">MSCRLAGMAASVLLWAGRAVVGSWRVGLAVVDIGDIGGKGLLGTLVRMAHSPPWVVGWSAGYLHTAGFNQSRLILAVHPRSAHSTQYAIAITAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H684</accession>
    <name>A0A1A0H684_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_13232</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709998.1">
        <property type="nucleotide sequence ID" value="XM_018854324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H684"/>
    <dbReference type="STRING" id="869754.A0A1A0H684"/>
    <dbReference type="GeneID" id="30027300"/>
    <dbReference type="OrthoDB" id="674604at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059122">
        <property type="entry name" value="Beta-prop_WDR5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051179">
        <property type="entry name" value="WD_repeat_multifunction"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857:SF21">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 4 WD40 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19857">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25175">
        <property type="entry name" value="Beta-prop_WDR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR5-like beta-propeller" evidence="5">
        <location>
            <begin position="42"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="79"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="122"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="164"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="228"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="357"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="386"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="433"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="442" mass="47419" checksum="CF07EDC5811FB70D" modified="2016-10-05" version="1">MGEPAEKKSKLISTEQETGAAEQFSLDQLYVQKYSLAVCTEELINAVKISKDRRILATCSSKGVIRAYNFEDGSLLATLRGHTKGVSDIAFSPINSDIVASGSDDLTIRLWSLSQRKCIRILKKHTYHITTLTFNSKGSMLISGAADETIVVWDLTTGNSLKTLAAHSDPVSSVCLTPDDTIIVSSSYDGLMRLFDTETGQCLKTLIYNSTSHGTATASTSDVVNFPISKVTISPNGKYILSSSLDGKIRLWDYMNNKVVKTYLGVGGTAINNKYNSGSAFVQKSSVPIVVSGSDCCGTLFWDLQSKAIVYQLQPGQTILDVMTIDGGAFLATCSLSGAVEVFELNQKYAASTMKDCSMDDQRSGAGNDSRQSPNESVKNESASGTPAETPAETPAEITVETPVETHAKTHGETPAEAPTELPADIHTNGCGEPREHKPAEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6B6</accession>
    <name>A0A1A0H6B6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_46258</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710099.1">
        <property type="nucleotide sequence ID" value="XM_018858018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6B6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6B6"/>
    <dbReference type="GeneID" id="30030994"/>
    <dbReference type="OrthoDB" id="194468at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016814">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01298">
        <property type="entry name" value="ATZ_TRZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000014">
        <property type="entry name" value="5-methylthioadenosine/S-adenosylhomocysteine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054418">
        <property type="entry name" value="MQNX/HUTI_composite_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22039">
        <property type="entry name" value="HUTI_composite_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminodeoxyfutalosine deaminase/Imidazolonepropionase-like composite" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydrolase-related" evidence="4">
        <location>
            <begin position="55"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF22039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="451" mass="48988" checksum="01DE9E2D5E2D5E82" modified="2016-10-05" version="1">MIYTNATIITVNSNFDVIDNGAIVVQADRIAAVGPADEILQKFKDEEIFDLGGQIVMPGLISLHVHLAQSLLRTAADDLALIEWLCHRVWRMQGNFTEEDGYVAAQLTIAEMLKSGTTTFVESLFAERYGFAGAVDAVVESGIRGCLGKVVMDQPRYATQEGITMHAGLVEDERSLDHAVQCFDKHDGAGDGRVEVWFGARTPGGVSEDLYRRMVKIARARNIGITMHCAEVKADREFFASKGHTPMSYCKELGLLGPRTVLAHMVHLDDTDIGILSKSSASVAHCPTSNAKLGSGVARVKELLEANVTVGLGCDGCPCNNVMDLLQEMKLASLLPKAIHGDPLLVPARTVIEMATIMGARALGKDLDIGSLEVGKKADFISINLVDKLYAQPMRDPVSMVVYIATGADVDNVVVNGKQVIQNRRLLTMDERDIIQKANFHGEAVRQRANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6E2</accession>
    <name>A0A1A0H6E2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_79542</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710058.1">
        <property type="nucleotide sequence ID" value="XM_018859084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6E2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6E2"/>
    <dbReference type="GeneID" id="30032060"/>
    <dbReference type="OrthoDB" id="1045173at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990856">
        <property type="term" value="F:methionyl-initiator methionine tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="97"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="527" mass="57394" checksum="8E097895788DD90C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6N1</accession>
    <name>A0A1A0H6N1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5 7">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5 7"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710095.1">
        <property type="nucleotide sequence ID" value="XM_018859091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6N1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6N1"/>
    <dbReference type="GeneID" id="30032067"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006806">
        <property type="entry name" value="FTHF_cligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="6"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="143"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="201" mass="22532" checksum="29882219B8D37946" modified="2016-10-05" version="1">MSRLAKNDLRKAIKTKLSHLSEARLAEQSQSVFDALIKHQEFRNAKSVALFMSMPRVETSTGPILRYCFDHEKTVFLPKCLPPSSLGNSMTFLNVKTLDEVNALQPAGKYKLREPAQGEDAMETGNLDLIIVPGVAFTLLGNRLGHGAGYYDKFLHDFQAKFDRVPYLMGVCLAEQLAENVPIEPHDWTMNHVIVGRGMQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S5</accession>
    <name>A0A1A0H6S5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Xaa-Pro aminopeptidase</fullName>
            <ecNumber evidence="5">3.4.11.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_33260</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710140.1">
        <property type="nucleotide sequence ID" value="XM_018856255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S5"/>
    <dbReference type="GeneID" id="30029231"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071281">
        <property type="term" value="P:cellular response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000015">
        <property type="entry name" value="Xaa-Pro aminopeptidase app-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000007">
        <property type="entry name" value="Xaa-Pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="14">
        <location>
            <begin position="79"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="13">
        <location>
            <begin position="419"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="15">
        <location>
            <begin position="642"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="704" mass="78604" checksum="406A6FDAAA586FC0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Y1</accession>
    <name>A0A1A0H6Y1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_45410</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710379.1">
        <property type="nucleotide sequence ID" value="XM_018857915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Y1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Y1"/>
    <dbReference type="GeneID" id="30030891"/>
    <dbReference type="OrthoDB" id="15954at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000002">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000003">
        <property type="entry name" value="Leucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="14">
        <location>
            <begin position="70"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="15">
        <location>
            <begin position="252"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="463"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="663"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="764"/>
            <end position="866"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="933" mass="104820" checksum="4CBBEC01ECA8561D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H724</accession>
    <name>A0A1A0H724_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">UvrD-like helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_12760</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710232.1">
        <property type="nucleotide sequence ID" value="XM_018854264.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H724"/>
    <dbReference type="GeneID" id="30027240"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035649">
        <property type="term" value="C:Nrd1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033678">
        <property type="term" value="F:5'-3' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001147">
        <property type="term" value="F:transcription termination site sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045005">
        <property type="term" value="P:DNA-templated DNA replication maintenance of fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060257">
        <property type="term" value="P:negative regulation of flocculation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21408">
        <property type="entry name" value="1B_Sen1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18042">
        <property type="entry name" value="DEXXQc_SETX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001152">
        <property type="entry name" value="tRNA-splicing endonuclease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044340">
        <property type="entry name" value="Helicase_Sen1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024481">
        <property type="entry name" value="Helicase_Sen1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056474">
        <property type="entry name" value="SEN1_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF495">
        <property type="entry name" value="HELICASE SENATAXIN ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23576">
        <property type="entry name" value="SEN1_barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12726">
        <property type="entry name" value="SEN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="11">
        <location>
            <begin position="1246"/>
            <end position="1561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="917"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="951"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1781"/>
            <end position="1983"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1413"/>
            <end position="1447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="923"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="961"/>
            <end position="970"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1781"/>
            <end position="1794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1804"/>
            <end position="1818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1835"/>
            <end position="1846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1873"/>
            <end position="1893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1900"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1927"/>
            <end position="1953"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="1267"/>
            <end position="1274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1983" mass="222056" checksum="8CD8D3420E82E504" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H731</accession>
    <name>A0A1A0H731_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DUF572-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_15881</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710290.1">
        <property type="nucleotide sequence ID" value="XM_018854620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H731"/>
    <dbReference type="STRING" id="869754.A0A1A0H731"/>
    <dbReference type="GeneID" id="30027596"/>
    <dbReference type="OrthoDB" id="674963at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000384">
        <property type="term" value="F:first spliceosomal transesterification activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000349">
        <property type="term" value="P:generation of catalytic spliceosome for first transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000350">
        <property type="term" value="P:generation of catalytic spliceosome for second transesterification step"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111:SF1">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="221" mass="26211" checksum="E7C923D634329A9E" modified="2016-10-05" version="1" fragment="single">MSERKAINKYYPPDWDPSKVPKKKKNTNPNAEKVRLMVPFSMKCLQCLEYISSRRKFNARKEITNEKYMGIKIIKFHIKCPRCNFSLLFQTDPKSAGFIPVAGCTRNYESLSTTEAIKPLETEDEILERLERQEKEDQQFQEQQQKRKNNPFWLRQTLDSSKDIMSNLEDKLIEQKREQEVHDHLLYLQAKANKLQQSGGEDYLVNHAQQKLRVDSKRDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7K9</accession>
    <name>A0A1A0H7K9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">60S ribosomal protein L12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_13036</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710536.1">
        <property type="nucleotide sequence ID" value="XM_018854294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7K9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7K9"/>
    <dbReference type="GeneID" id="30027270"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="7">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="6">
        <location>
            <begin position="74"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="165" mass="17829" checksum="E9AB66FF34A958B6" modified="2016-10-05" version="1">MPPKFDPSEVKFLYLRAVGGEIGASSALAPKIGPLGLSPKKVGEDIAKATKDFKGIKVTVQLRIQNRQATASVVPSASSLVITALKEPPRDRKKEKNVKHSGNIPLDQIFEIARQMKDKSFGKNLASVTKEILGTAQSVGCRVDYKNPHDIIEAINAGEIDVPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Q8</accession>
    <name>A0A1A0H7Q8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Clathrin adaptor, mu subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_44752</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710452.1">
        <property type="nucleotide sequence ID" value="XM_018857835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Q8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Q8"/>
    <dbReference type="GeneID" id="30030811"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="197"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="443" mass="50083" checksum="CC2150A6363F52C9" modified="2016-10-05" version="1">MITSLLIFNSKGDVLMSKFYKEGVKKNVADVFRIQVINSAGKISASTAHASRLPVLTLGSTSFLYIRSGYLWLVAVARSNQDSSLIFEFLHHLLHLLDQLFARGADQLSEDDIKGNFSMIFDILGEIVEFGYPTNMDYAYLASVVPGFSSLKVSHKDGEFSSLKKSLLEKSDQLNSIDLVYDPSKVSWREQGIKYRKNEIFLNVDEKISLLLDSRGNHLRSHIDGTITLKSHLSGTPVCRFGLSDDYLRDVTQSISVDDFNFHKCVDLAKYTTEGVIRFVPPDGTFQLMSYRINDTFPLPFDINPEIVEDGGGIYLKIRMLSNFPSKAAATGVVLKIVIPQGIMKRTISCSNGKSKYDAQENAILWKFSKFYGELEQLISVDFEVPDTTTAWFKPKITLDFNMDMYSASGLQVQFLKVVEKNNYRTVKWVKYRTQAGSYEIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7X8</accession>
    <name>A0A1A0H7X8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710528.1">
        <property type="nucleotide sequence ID" value="XM_018854293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7X8"/>
    <dbReference type="GeneID" id="30027269"/>
    <dbReference type="OrthoDB" id="4076147at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="39042" checksum="EC20F98368D77002" modified="2016-10-05" version="1">MDKEPISSDKISSPASPKDTQEEDPDDIPILQSKKAELIQERDILAQKRLELKQSIERLSASLSFYREQQLQHETKQKLEYYLHKNDHECSKLTQPDEAASFILKNLDVLPTTDVHLRMKLLNRFYPCMSVAQEHVLTIHEEDVLFTVIRFKIFAHGLPPLAIRLHSNDDAVHRLELLDYSEVAPIFQKVSPSFCKTLINNYCRQKKIDMIMHSYHSLAKLQQSRIMCLSQLLLKYADLVTRPKSWASYPELSLMSLNHIELTIPESTNRDRHIVQLSWEMILKNTAIGEMESQLRFSVQRRDSGPLKNVNEVFLSLMLEHGIMNAFSLMLSNLFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H829</accession>
    <name>A0A1A0H829_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Winged helix DNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_44754</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SNF8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710701.1">
        <property type="nucleotide sequence ID" value="XM_018857836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H829"/>
    <dbReference type="STRING" id="869754.A0A1A0H829"/>
    <dbReference type="GeneID" id="30030812"/>
    <dbReference type="OrthoDB" id="283883at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000814">
        <property type="term" value="C:ESCRT II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043328">
        <property type="term" value="P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000397">
        <property type="entry name" value="Vacuolar-sorting protein SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016689">
        <property type="entry name" value="ESCRT-2_cplx_Snf8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040608">
        <property type="entry name" value="Snf8/Vps36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806">
        <property type="entry name" value="EAP30 SUBUNIT OF ELL COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12806:SF0">
        <property type="entry name" value="VACUOLAR-SORTING PROTEIN SNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04157">
        <property type="entry name" value="EAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="236" mass="26493" checksum="8C0910AB2B8D273F" modified="2016-10-05" version="1">MTPERDAYLRLGKTLTERHSDQLAIQLSVFQSALINFGVEHGDAIRNNAEFRHKFTQMCALIGVDPLELLIMTDSRAKKTDGFFVGLGVRMVEICQETRNINGGLISFKELHSRLQESVSVPLAVSEADAQKALDSLVSLGKGYETLSINNKQWIKFLGPTGSQSISNDQKKVYELCEFMGGHVTYRLLRDNFGWDKVRTNTVVDEMIMNGFLWVDAQGPGGATQFWEPSWISKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H865</accession>
    <name>A0A1A0H865_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GroES-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_79456</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YIM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710700.1">
        <property type="nucleotide sequence ID" value="XM_018859070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H865"/>
    <dbReference type="STRING" id="869754.A0A1A0H865"/>
    <dbReference type="GeneID" id="30032046"/>
    <dbReference type="OrthoDB" id="3509362at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050700">
        <property type="entry name" value="YIM1/Zinc_Alcohol_DH_Fams"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11695:SF294">
        <property type="entry name" value="RETICULON-4-INTERACTING PROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13602">
        <property type="entry name" value="ADH_zinc_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="4">
        <location>
            <begin position="20"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="363" mass="39034" checksum="B342E05FF3A940E1" modified="2016-10-05" version="1">MLEFRAIKYKNKTLDFAVVADSLPVQVQNGQYQLPSEDDFLVKVHAAALNPVDLIIKNSLSPWLFRGTRGFGMDYSGDIVAIGRQAAAATGLAVGDRIAGLYQKPLGAGTISEYLLVDHKKPEGRNARKLPDSMSYQEGAAYPLVFGTAQTMFDTVAKGNSFGKVLVLGAGTAVGRHCVQLALKVYGAEHVVVTCSARTEHTIRALGATSVIDYTQHPSILNPVLEAVKETGPFDVVLDCCGNSDLFPQMAAVLKPRGEAGSYVSIVGDTKANFKTSSIGGLVLGSFWVTVRMVRNTLGLLPYFYSQALLDQGGSWPDKCIKNLSEGKVRVFIDSEYAMADTHKAVDRLLSNQASGKVIVNVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C8</accession>
    <name>A0A1A0H8C8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylthioribulose-1-phosphate dehydratase</fullName>
            <shortName evidence="7">MTRu-1-P dehydratase</shortName>
            <ecNumber evidence="7">4.2.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MDE1</name>
        <name evidence="9" type="ORF">METBIDRAFT_43380</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-(methylsulfanyl)-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15549"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="4.2.1.109"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aldolase class II family. MtnB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.109" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710757.1">
        <property type="nucleotide sequence ID" value="XM_018857650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C8"/>
    <dbReference type="STRING" id="869754.A0A1A0H8C8"/>
    <dbReference type="GeneID" id="30030626"/>
    <dbReference type="OrthoDB" id="191080at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00875"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046570">
        <property type="term" value="F:methylthioribulose 1-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000003">
        <property type="entry name" value="Methylthioribulose-1-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03116">
        <property type="entry name" value="Salvage_MtnB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017714">
        <property type="entry name" value="MethylthioRu-1-P_deHdtase_MtnB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027514">
        <property type="entry name" value="Salvage_MtnB_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03328">
        <property type="entry name" value="salvage_mtnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10640:SF7">
        <property type="entry name" value="METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="8">
        <location>
            <begin position="14"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="250" mass="28210" checksum="C2D963068F4B1264" modified="2016-10-05" version="1">MASFFSDHKNHPANVICELCRLFYDNNWVTGTGGGISIRDVDGENPDIVYIAPSGIQKERLHPKEMFVAELPDKILRSPNEDNEKEPLSLDLAANFKYKPLACTPLFLSIYNLRDAGACIHTHSQNAVLATMIWEDKLVFEMNNQEQIKALPKLEMNVATGKIEKVGSLHNHETMVIPIVDNTPLEEDLTETLEATLKKYPGATAVLVRRHGIHVWGENIWKAKIYNEAIDYLLELAIKMHQNGIPLIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8F1</accession>
    <name>A0A1A0H8F1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Histidinol-phosphatase</fullName>
            <shortName evidence="8">HolPase</shortName>
            <ecNumber evidence="3 8">3.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_44099</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-histidinol phosphate + H2O = L-histidinol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14465"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="3.1.3.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PHP hydrolase family. HisK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.15" evidence="3 8"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710823.1">
        <property type="nucleotide sequence ID" value="XM_018857740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8F1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8F1"/>
    <dbReference type="GeneID" id="30030716"/>
    <dbReference type="OrthoDB" id="5957391at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00013"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004401">
        <property type="term" value="F:histidinol-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12110">
        <property type="entry name" value="PHP_HisPPase_Hisj_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010140">
        <property type="entry name" value="Histidinol_P_phosphatase_HisJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01856">
        <property type="entry name" value="hisJ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039">
        <property type="entry name" value="HISTIDINOL PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21039:SF0">
        <property type="entry name" value="HISTIDINOL-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHP" evidence="9">
        <location>
            <begin position="4"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="312" mass="35272" checksum="04D19A291736C416" modified="2016-10-05" version="1">MHSHHSHSGEYISHGEGSLESMVATAQARKFSHYCLTEHMPRLSGKYLYPEEIEKNYTAENLDTDFSKYLTHARKIQTRLRDAGSLQVLVGFEVEGIDGPHIRAAKSIRQKTDMCVGSVHYVHGIPIDFSAELWLQARAACADNTSRCLYKDYFDLQYQVLTELHPHVVGHFDLIRLCEASEIDSSSGIHIRSIDIEQDWPDVWQAITRNIQYVVSYGGLFELNSSAVRKGWVSPYPRLDIAQAIKRLGGMFCLSDDAHTTAQVGLNYDKVWAYVVDELRLSHVHHLELDAAGNTVVVADRVAELARLSFWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8J6</accession>
    <name>A0A1A0H8J6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_43475</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710961.1">
        <property type="nucleotide sequence ID" value="XM_018857663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8J6"/>
    <dbReference type="STRING" id="869754.A0A1A0H8J6"/>
    <dbReference type="GeneID" id="30030639"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000297">
        <property type="term" value="F:spermine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000011">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF132">
        <property type="entry name" value="POLYAMINE TRANSPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="361"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="132"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="570" mass="62834" checksum="0BF2BD74644A2FEF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Q4</accession>
    <name>A0A1A0H8Q4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711021.1">
        <property type="nucleotide sequence ID" value="XM_018854232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Q4"/>
    <dbReference type="GeneID" id="30027208"/>
    <dbReference type="OrthoDB" id="10385711at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="353" mass="39492" checksum="298722E861A65411" modified="2016-10-05" version="1">MYMREVGPATQGVDGRSRELDALQKHLCSPDPGRFQEALDLMERLGHEGLGLQSTPVSRALAHRTNTEYDKTHSLFDCSDVTSVDTCGQFRDTDRDSDLDETASLMECGEAMHANLDSSGPGPGGSPSQFNLYEFLDGIQTRHVTISSKFQETHAKLDVLRHQNRLDADLLAKLSANNELMHEKIHGLKTEVSDMFHDLHLLKTSRERGGPPGCRPNPTYTLAYTSLPDRCTTISDMAKTDSTTVDTHFCFIADSEENDRGKAAPIMHCHGVSKHPVASQSASPHSGTLSTQMDETTQIHSIQFDKAPVGPNMMPKYAKEMLALFVLLLAFWLALSWHWTHYTWDIKWMQKYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8V6</accession>
    <name>A0A1A0H8V6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_200501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711076.1">
        <property type="nucleotide sequence ID" value="XM_018854929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8V6"/>
    <dbReference type="GeneID" id="30027905"/>
    <dbReference type="OrthoDB" id="4022940at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="597" mass="69018" checksum="0FCDCE1ACDDAC1B0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H914</accession>
    <name>A0A1A0H914_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_197388</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710895.1">
        <property type="nucleotide sequence ID" value="XM_018854886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H914"/>
    <dbReference type="GeneID" id="30027862"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="13854" checksum="E557DC9456536E74" modified="2016-10-05" version="1">MKDPLIAPVFEFLLFYCCYCQPPDLMEGFLFLARVDGYGQLPSMVFMGAVSTFGCFQPSSRFDGCSFFNLDFGNYTKVLRPAQHSLISDGRNSHCNLLRVLMAINENEQTPAPKFEETPGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H948</accession>
    <name>A0A1A0H948_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711047.1">
        <property type="nucleotide sequence ID" value="XM_018854234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H948"/>
    <dbReference type="STRING" id="869754.A0A1A0H948"/>
    <dbReference type="GeneID" id="30027210"/>
    <dbReference type="OrthoDB" id="5431245at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037738">
        <property type="entry name" value="Ecm13-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36826">
        <property type="entry name" value="PROTEIN ECM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36826:SF1">
        <property type="entry name" value="PROTEIN ECM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="243" mass="27056" checksum="A3C912B250F54133" modified="2016-10-05" version="1">MSETDDMSFASSYVLASKVRSKLTKQASSPTSSLRQLVLQANMLDNIMDHISSKTEQMRNKKSVSFSTPQFHNYKKHSAIEQTNGPNVTEYEVDSDSDSDFDSDSESEADNVEYASGNDDDDYYFSSDSDEGDEEIENMTSLSYAHKNIMPTIDLSDDSQLLVIYEEHEDALPELTHLVSSSDSDSDDDIEMPSYILSSAHANRSTESLLGADISVKDGGDHSSPMRHHHERHSAICSIEDVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H960</accession>
    <name>A0A1A0H960_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_32532</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711068.1">
        <property type="nucleotide sequence ID" value="XM_018856069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H960"/>
    <dbReference type="STRING" id="869754.A0A1A0H960"/>
    <dbReference type="GeneID" id="30029045"/>
    <dbReference type="OrthoDB" id="434619at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08829">
        <property type="entry name" value="SPFH_paraslipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050710">
        <property type="entry name" value="Band7/mec-2_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="STML2-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF10">
        <property type="entry name" value="STOMATIN-LIKE PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Band 7" evidence="4">
        <location>
            <begin position="43"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="319" mass="35634" checksum="64CDF524ECBDF1E6" modified="2016-10-05" version="1">MNSTRIIRSPSFGVSRWYSSFNNTRLGPSALSFFQKERLPTNTLVKFVPQQTAYVVERMGKFHKILEPGVAIMIPILDKITYVQSLKETAIEIPSQNAITSDNVSLELDGILYVKVHDPYKASYGVEDFKFAISQLAQTTMRSEIGSMTLDSVLKERQHLNSNINGIINEAAKEHWGVQCLRYEIKDIHPPQNVLEAMHRQVSAERSKRAEILESEGTRQSKINIAEGEKQSMILQAEATAISIEKIANSIKNTPGGMDAINLQVAQQYIKEFGKIAKETNTIVLPSNLNDLNGLMATGLSLFKSMNKQQTVTSSKSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H978</accession>
    <name>A0A1A0H978_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_43990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ornithine cyclodeaminase/mu-crystallin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710964.1">
        <property type="nucleotide sequence ID" value="XM_018857729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H978"/>
    <dbReference type="STRING" id="869754.A0A1A0H978"/>
    <dbReference type="GeneID" id="30030705"/>
    <dbReference type="OrthoDB" id="41492at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="entry name" value="ornithine cyclodeaminase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812:SF19">
        <property type="entry name" value="KETIMINE REDUCTASE MU-CRYSTALLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="37930" checksum="FB1C08911689CF16" modified="2016-10-05" version="1">MKVISDSAITAYLMENLDKDTILNVYVPLLLSGLTKFQQFPDAVPERTVRVSTTPDSDSTHLFMPCILPEIVGVKVISGGPSNLAKGLGFQGNVSVLDEYLGELLAVLNAKTLTAFRTALASCVSMVKVFDPKLANENLLSLLVFGAGPQAFWHVILATRLYPNLAQVNIISRTSESAKTLAHKLRGVITQKLLVFGFEDVLSIEASVRESSLIFGCTPSTDAIIKSTYINSDPGKTTFVSLIGSYKPHMIELDLQFLREFYTGQSTKIIVDSKSHCLSEAGELIQGNISESQLISLTELDEKSVDIKDIGKSRCGVVLQKLVGLLVMDIVVAKHFARHIEGHAIDDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9B3</accession>
    <name>A0A1A0H9B3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78845</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat rae1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710999.1">
        <property type="nucleotide sequence ID" value="XM_018858957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9B3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9B3"/>
    <dbReference type="GeneID" id="30031933"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000728">
        <property type="term" value="P:regulation of mRNA export from nucleus in response to heat stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000190">
        <property type="entry name" value="Nuclear pore complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="71"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="265"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="338"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="345"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="366" mass="40166" checksum="AB09B14EA4F2F780" modified="2016-10-05" version="1">MSFLNSTSTQNSAATGQELVNDITLNNGPEDTVTDLAFSPVLDMLAVASWDKKVRIYDIDPNSGNSQGRALFEHDGPVFSARWTADGARIASGGADKQVKLFDLASQQLQQIGAHDAPVRCVRSVLCGPTNTEVVVSGSWDKTLKYWDMRAPQPVSVINLPEKVYCMDSSQKLLVVGCAERHVAIIDLTNPQQVFKSIMSPLKYQTRSIACYPQGNGYAIASIEARCAIQYIDDAEQQKAGFSFRCHRKGANGANPPRTTAVGASETHLYTVNAISFHPIHGTFSTAGSDGTFCFWDKDAKQRLKNFPNVGAPITATAFNRNGSIFAYARGYDWSQGHQGNRPDYPTDVKLHPLKDEEIKQKKKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9B6</accession>
    <name>A0A1A0H9B6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_199467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710990.1">
        <property type="nucleotide sequence ID" value="XM_018854913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9B6"/>
    <dbReference type="GeneID" id="30027889"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="75" mass="8978" checksum="1CF8D5A082FE31DB" modified="2016-10-05" version="1">MVFTSCSGVSFHLLFRYIFLFQIDNLSSFYLFFKKKSHKIMHYRNIPIINISSYYMDGYIQIMNERAYGRSLISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9F7</accession>
    <name>A0A1A0H9F7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_205585</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 15">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711276.1">
        <property type="nucleotide sequence ID" value="XM_018854975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9F7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9F7"/>
    <dbReference type="GeneID" id="30027951"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF137">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="209"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="50"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="472"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="529"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="683" mass="76982" checksum="74D15937FEDBEDA1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9S6</accession>
    <name>A0A1A0H9S6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_201688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711150.1">
        <property type="nucleotide sequence ID" value="XM_018854940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9S6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9S6"/>
    <dbReference type="GeneID" id="30027916"/>
    <dbReference type="OrthoDB" id="10067394at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="958" mass="108201" checksum="7FEB8046BE0054D7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W6</accession>
    <name>A0A1A0H9W6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Plus-3-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_43641</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711190.1">
        <property type="nucleotide sequence ID" value="XM_018857683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9W6"/>
    <dbReference type="GeneID" id="30030659"/>
    <dbReference type="OrthoDB" id="166375at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990269">
        <property type="term" value="F:RNA polymerase II C-terminal domain phosphoserine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070911">
        <property type="term" value="P:global genome nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031126">
        <property type="term" value="P:sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001015">
        <property type="term" value="P:snoRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13115:SF8">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN RTF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00719">
        <property type="entry name" value="Plus3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51360">
        <property type="entry name" value="PLUS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Plus3" evidence="6">
        <location>
            <begin position="214"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="102"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="168"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="420"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="535" mass="60565" checksum="2C0F20334BA42F1E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA10</accession>
    <name>A0A1A0HA10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nuclear pore complex protein Nup85</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_11465</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Functions as a component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the nuclear pore complex (NPC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the nucleoporin Nup85 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711361.1">
        <property type="nucleotide sequence ID" value="XM_018853997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA10"/>
    <dbReference type="STRING" id="869754.A0A1A0HA10"/>
    <dbReference type="GeneID" id="30026973"/>
    <dbReference type="OrthoDB" id="17644at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011502">
        <property type="entry name" value="Nucleoporin_Nup85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373">
        <property type="entry name" value="FROUNT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13373:SF21">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07575">
        <property type="entry name" value="Nucleopor_Nup85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7 9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="737" mass="83894" checksum="01FBD93413687FD0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAE9</accession>
    <name>A0A1A0HAE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">V-type proton ATPase proteolipid subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77996</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10 11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Vacuole membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711362.1">
        <property type="nucleotide sequence ID" value="XM_018858800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAE9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAE9"/>
    <dbReference type="GeneID" id="30031776"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18175">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18176">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6C_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.610:FF:000001">
        <property type="entry name" value="V-type proton ATPase proteolipid subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011555">
        <property type="entry name" value="ATPase_proteolipid_su_C_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01100">
        <property type="entry name" value="V_ATP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="12">
        <location>
            <begin position="91"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="160" mass="16365" checksum="4B169C808D9AA1C1" modified="2016-10-05" version="1">MSDLCPVYAPFFGAIGCTAAIVFTCFGASYGTAKSGVGVCATCVLRPDLLFKSIVPIILAGIIAIYGLVVSVLITDSLKQTQALYTGFIQLGAGLSVGLSGLAAGFAIGIVGDAGVRGNAQQPRLFVGMLLILIFAEVLGLYGLIVALLLNSRATQDVTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB11</accession>
    <name>A0A1A0HB11_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_179786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711824.1">
        <property type="nucleotide sequence ID" value="XM_018854760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB11"/>
    <dbReference type="GeneID" id="30027736"/>
    <dbReference type="OrthoDB" id="4096130at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="256" mass="27224" checksum="6DE70F6B251923CF" modified="2016-10-05" version="1">MPHEDSQAGYLSANSSKAAAMILNGHYNYPGPKNAYVRAIPQEVDINERLNIGDLPAMPRPRVSEELFADESARTQCASLENLVISFDGPLNDLLADSQAKISQYAVELPSARARRTNPSGGPLCSGDAGASGHFPTPTLLSQEVEPGRGQPQPEHQVDGDKEASATSADPLACHANNIMRMAMDSTMLGSFCNDTISSSFSNVVDESIPGAAGERSFGEDDVARRAPSLRFLGATKVEISAHDLEMAVSDPLRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB41</accession>
    <name>A0A1A0HB41_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_180921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711854.1">
        <property type="nucleotide sequence ID" value="XM_018854769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB41"/>
    <dbReference type="GeneID" id="30027745"/>
    <dbReference type="OrthoDB" id="4096464at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="162" mass="17739" checksum="27719DDA60E82FDE" modified="2016-10-05" version="1">MMTFTLENDLDEFSTRYWECCQEVITKQTRVGMLVSRLSSPLPVTESGDSASKSTSSASDSKNEAHDTGLKHLLTSVQSSPKRLRVETHGLKGETPSKSGESVSALNPLAGDTSWRPSPAGKLLPANPRPRPCKMLTSRLKSIGNPFYKQRNKDTSLSSTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB57</accession>
    <name>A0A1A0HB57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNA polymerase II-associated</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_31719</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711622.1">
        <property type="nucleotide sequence ID" value="XM_018855900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB57"/>
    <dbReference type="STRING" id="869754.A0A1A0HB57"/>
    <dbReference type="GeneID" id="30028876"/>
    <dbReference type="OrthoDB" id="10260285at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="387"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="432" mass="48390" checksum="87AC03516885D75A" modified="2016-10-05" version="1">MSKPPKPVRQDYIAKVRYINHLPPPPLNPKFLRYNSTQPLSAQAENEALLSSLFRKDNFNAYIAQVDEEYGMGLNPLLAPGFLDAPAAAALAAHTPALHPKDRAFLRDAGVGKINKSEPGVSFLRRTEYIAERQSAARPDEKGPANTAADDAGDRVDPDSQLRAVEQSFEESQKSLAAYGALRHPRKTKLRAVLVFPLLPDTASMDTTFLAVKFAGSASMARERQAQCSREKARFDEALHADALACAIYKPITSEDGEWVSFYQMKDRQAVRRLRLRLESTEKEQPANLLDGEDAREAAFRFRHHKNYDMLYHPFSKPYEELALRFVSDAPAGGEPAPRKRRAAYYCPISGRVELKKHRASANTEINRFLADSTMDAIDFRLREPNTNELRKMDNARSEYDPMEYEGDDDDDDDDEPEAKEAEAPGGAEGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB95</accession>
    <name>A0A1A0HB95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_25987</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711673.1">
        <property type="nucleotide sequence ID" value="XM_018855308.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB95"/>
    <dbReference type="STRING" id="869754.A0A1A0HB95"/>
    <dbReference type="GeneID" id="30028284"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="137" mass="13633" checksum="682DCD9BF2EA5818" modified="2016-10-05" version="1" fragment="single">TAGTTVVTVDVTVTDYTTYCPASTVVTVTTCSNDQCAPTTVTVSEATTLTVTGECVVESTLTTAASSTLGSNFSTVSEFSSAFITQASSTLVTQISSSLESTTIGLSNSSSITTYEDGAGKAAIGAAGLVGVVAFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBG9</accession>
    <name>A0A1A0HBG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Exportin-T</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Exportin(tRNA)</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA exportin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_11779</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">tRNA nucleus export receptor which facilitates tRNA translocation across the nuclear pore complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <text evidence="9">Shuttles between the nucleus and the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the exportin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711732.1">
        <property type="nucleotide sequence ID" value="XM_018854082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBG9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBG9"/>
    <dbReference type="GeneID" id="30027058"/>
    <dbReference type="OrthoDB" id="26399at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045546">
        <property type="entry name" value="Exportin-T_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040017">
        <property type="entry name" value="XPOT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952:SF11">
        <property type="entry name" value="EXPORTIN-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15952">
        <property type="entry name" value="EXPORTIN-T/LOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19282">
        <property type="entry name" value="Exportin-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <keyword evidence="6 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Exportin-1/Importin-beta-like" evidence="10">
        <location>
            <begin position="103"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Exportin-T C-terminal" evidence="11">
        <location>
            <begin position="318"/>
            <end position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="991" mass="112811" checksum="A1340C5ED410DD73" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBV4</accession>
    <name>A0A1A0HBV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glycoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_42747</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711867.1">
        <property type="nucleotide sequence ID" value="XM_018857581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBV4"/>
    <dbReference type="GeneID" id="30030557"/>
    <dbReference type="OrthoDB" id="4082933at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF24">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291733" evidence="9">
        <location>
            <begin position="17"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="126"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="444" mass="47819" checksum="2688121738B48B97" modified="2016-10-05" version="1">MIYLLTLLVLLQVIVAAPLPLVVTRYHEADTVTSTKTYITGTTTIWLPPVEIFISNGVRYTFTKYDGQWATTQATTTSVYTEETSDTPAGQATPTAVVPTLETAQAAETTTPIPAAVTSAVPPTKATTEATTEATTEATTEATTEATTQTTTEATTQTTTATPTQTSTSSTSTSVASGYISPPSVIVYSPYNNDGTCKTYDVIESDLNFVKSKGISKVRLYGADCYVVEAVLPLCLELGITVNQGFWISSEGVDSIDDYVTLVVAWAKTNGWDVFDFFTVGNEAVNENYCTVSELISKIASVKALLSDAGWTGQITTSEPPVTFINNPSLCTESEIDFVGVNPHSYFNTNLYAYQAGEYITAQQAQVAKLCSKSAFITETGYPHKGDVYGNNVPSKENQLIALQTIMEYTGGDVTILTLYDDMWKSPGVHGIEQYFGMIDLVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBX0</accession>
    <name>A0A1A0HBX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sister chromatid cohesion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_40914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCC2/Nipped-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712144.1">
        <property type="nucleotide sequence ID" value="XM_018857329.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HBX0"/>
    <dbReference type="GeneID" id="30030305"/>
    <dbReference type="OrthoDB" id="418242at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090694">
        <property type="term" value="C:Scc2-Scc4 cohesin loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061775">
        <property type="term" value="F:cohesin loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140588">
        <property type="term" value="P:chromatin looping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034087">
        <property type="term" value="P:establishment of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071169">
        <property type="term" value="P:establishment of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024986">
        <property type="entry name" value="Nipped-B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033031">
        <property type="entry name" value="Scc2/Nipped-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704:SF18">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21704">
        <property type="entry name" value="NIPPED-B-LIKE PROTEIN DELANGIN SCC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12830">
        <property type="entry name" value="Nipped-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Sister chromatid cohesion C-terminal" evidence="2">
        <location>
            <begin position="1232"/>
            <end position="1411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1529" mass="172140" checksum="2E0E8BA4032C8CE6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC12</accession>
    <name>A0A1A0HC12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA biogenesis protein RRP36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32031</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Associates with 90S and pre-40S pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the RRP36 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712030.1">
        <property type="nucleotide sequence ID" value="XM_018855966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC12"/>
    <dbReference type="STRING" id="869754.A0A1A0HC12"/>
    <dbReference type="GeneID" id="30028942"/>
    <dbReference type="OrthoDB" id="448446at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009292">
        <property type="entry name" value="RRP36"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21738:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING PROTEIN 36 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06102">
        <property type="entry name" value="RRP36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 9" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="50"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="277"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="301"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="313" mass="35893" checksum="0A2EC6CA776814EF" modified="2016-10-05" version="1">MNSRVRPDVSSGLDSENDLVGSVLRPAKSNFQDDSDDDFSALSFGALSSAQKKLMAEDRASRGPQKAPGKDREAPSKPGLLQKTSRGHKTGTARDRLQSAGPSDSELDDKEAFLEAQQPKTHKAKKSLQHRRNKHAPAEASLKRPVPKIREIAGLKTAKESTLYHDIRFDAAYGKADWSRIRKDYAFLDEYREQEIKDMQKTLKDKKSMLRMSARDADNLKFEMQSLKSRLDTLKNRDLASTIVLGHKKEQMHKMRTGEQVNPYFLKRSEQRKMVQKAKFDSMRAGQREKVMERKRKRRLGKEFKQLEFRDAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC50</accession>
    <name>A0A1A0HC50_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF1753-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_12053</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712070.1">
        <property type="nucleotide sequence ID" value="XM_018854155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC50"/>
    <dbReference type="GeneID" id="30027131"/>
    <dbReference type="OrthoDB" id="4095010at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508821" description="DUF1753-domain-containing protein" evidence="3">
        <location>
            <begin position="27"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="293"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="304"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="37559" checksum="482B6ABBA6C5881E" modified="2016-10-05" version="1">MLGVISPRPVLALLTFWWPLLLSVKALKAPGQNVQFLLTYWLFYACISGTQHLLAGLAFPLSSAINLILDFLHVWMFYSHGCLVALHHYIPSITGHTSAAAIVNALDHKVVDPLVSILVVRNRLLQRVLASRSSNVPPLDDFLLFNQSLCEHHALLPTRLSFLQFSLHYFCYVDLESELQARYAKSRGVLARFAPSLSSSVGGAASSKASSRVSSQTMQSRNVSVSSSRNGSLAWRSAPQTRPSSGRASRDVSSHSQRLHSQGLSQEMGPNSSLEERTYLLKPTRVSLSPAAHFDRAAERRHASMTTWPQSPNGSGKIHVRKRAKPRVSSSTAAELPYPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC60</accession>
    <name>A0A1A0HC60_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_188276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712085.1">
        <property type="nucleotide sequence ID" value="XM_018854813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC60"/>
    <dbReference type="STRING" id="869754.A0A1A0HC60"/>
    <dbReference type="GeneID" id="30027789"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="138" mass="15685" checksum="6017A85C9AEFC45B" modified="2016-10-05" version="1">MVSQLKTIKNLQIGLCQNQIEIREVLSVGLIESLADSKRELQTKKDILEQEILQKNHIAQTLANREVEANCHAEAILLLKAGIDTARRRREVRFESIDAPEQKLMDDSQKEEELIELAEERCARSEVTISDTDTSSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCC4</accession>
    <name>A0A1A0HCC4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative class II aldolase/adducin domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_40734</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712270.1">
        <property type="nucleotide sequence ID" value="XM_018857304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCC4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCC4"/>
    <dbReference type="GeneID" id="30030280"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="2">
        <location>
            <begin position="51"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="31330" checksum="C2C6122A86A232AB" modified="2016-10-05" version="1">MSPAPKITDKSDPKPQNLSERGSHNIARGMPHPYPLPKFDDKYEERKWAKQHMAGAFRVFARKGYTEGTAGHISLRDPVDPDTFWINPLAKHFGLIKASDLVHCDEAGNVLADGPQTAINAAGFAIHSAVHKARPDVISACHTHSTYGKAFSALGRPLDMISQDVCTFYRSHSVYTDFGGVAIDEEEGIAIASALGDGKGVILQNHGLLTVGATVDEAAYLFTLMEKSCECQLLADAALRPGEKMKLCGDEEAAYTEHMSGDPETLYTEFQPDFEMELYHNSDFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI8</accession>
    <name>A0A1A0HCI8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_12186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712220.1">
        <property type="nucleotide sequence ID" value="XM_018854188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI8"/>
    <dbReference type="STRING" id="869754.A0A1A0HCI8"/>
    <dbReference type="GeneID" id="30027164"/>
    <dbReference type="OrthoDB" id="5423336at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1102" mass="123184" checksum="8506EB485153E320" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCK0</accession>
    <name>A0A1A0HCK0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">FMN-linked oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_41398</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="8">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="9">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712230.1">
        <property type="nucleotide sequence ID" value="XM_018857395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCK0"/>
    <dbReference type="GeneID" id="30030371"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106414">
        <property type="term" value="F:mRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102264">
        <property type="term" value="F:tRNA-dihydrouridine20 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052582">
        <property type="entry name" value="tRNA-DUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45936:SF1">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(20) SYNTHASE [NAD(P)+]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 11" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="12">
        <location>
            <begin position="32"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="375" mass="42117" checksum="7ACB38753E953B5A" modified="2016-10-05" version="1">MVTYANKLCLAPMVRSGELPMRLMALKYGADLVWSPEIIDKKIMSCIRVENKDLGAIDFFELGKDNLRNSLVFRTKRSLEKGKLIFQLGSADPQLAVAGAEKVIKDVDGIDLNCGCPKPFSTHSGMGAALLSTPDLLVLILLNLVKEIGHPNGKPISAKIRLLNSSDAQPTLDLVERICKTGISNLTVHCRTREMRNRDAPIRNFVNEIHEVTKKHNVSLIINGAFRCKLEFKQFQKFMGNYEIGGMMAEAAESNPTVFCDRPLPWRDVIPEFIQFCISCDNHPSNTKYILLNQLPGKSRLYQAFCKIKKNDEFLKLANTIGEEGDKVFIRIRQKDKQYTAEELVFGQEETNPLKRAAENGATEALEKKQRTVCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCP6</accession>
    <name>A0A1A0HCP6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_39290</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712272.1">
        <property type="nucleotide sequence ID" value="XM_018857125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCP6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCP6"/>
    <dbReference type="GeneID" id="30030101"/>
    <dbReference type="OrthoDB" id="3066029at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005275">
        <property type="term" value="F:amine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF21">
        <property type="entry name" value="DITYROSINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="20"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="461" mass="50682" checksum="28D2D5D104BE065F" modified="2016-10-05" version="1">MLESEQGEVYTVFKTRKILFIFIATLCGSLGPLAANIFLPSIEVFQHVFHTSKQAINGTISMFMFVIAFAPLFLSLWSDNYGRRPLLLSSLSVYLIANILILTLPPNIIAFYIFRAFQGFGASNLAVGVGVIADISEPKNRAKAISYYMIGPQMGPVFGPMLGMVGARFSWRYNFIILSIFGGISLILVFFFLPETLRTIVGNGSDSSQKIIVPFSSRKANDLQAILNKPKRPGIMLYLRLCINRPVLLCAVSGGLAFSCFYAVTVSFSEALTNVYGFDVLHVCLAYIAPGVALVLGSIIFGILSDLLRKQDTLKYGIESRFKLQIACLLPFLGAVISFGWVVQSSGHFAYVILLTFLVAFLVSGILITNTTYLSELSRNQPASYVAFGNLIRNMGAAVFVACVNPMTNKVGYGWGFTIYALTCSVSFIFSILVLMFGSHWREALNRDTESVSEKDFQSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS4</accession>
    <name>A0A1A0HCS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_40591</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712400.1">
        <property type="nucleotide sequence ID" value="XM_018857292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCS4"/>
    <dbReference type="GeneID" id="30030268"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010658">
        <property type="entry name" value="Nodulin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF158">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 12-LIKE CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06813">
        <property type="entry name" value="Nodulin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="359"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="Nodulin-like" evidence="6">
        <location>
            <begin position="10"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="478" mass="50637" checksum="9628C42BAADB1F74" modified="2016-10-05" version="1">MIPSSVHKTLVLLSCTFLGLICGTLYLYLSYSPQLAQRLQYLATNALSIALVGSLGVAVSGPMAGLAVDRKGYTGALCAGGSLIVLGYFVLRRQYTHTYLNVALSCLCLFSVGAGSTLINLACLKCCAVTFPSLRGVATSLPLALYGLSAMFYLVVALVFYAGDTAGFLRFLMASVAALFALCSPCVMLCDRRPCLSGRTVRLADSIEMVNLRGLSEMKGALSPCATPTSGPADLHGLALLRLPRFWLLFLITGAMASLGQMYIYSVGYMVKALVIRNFDIDAAAATSLSVDMVIQNQQQVQVALLSMTNCVGRLVAGVMGDIISQTFRKLRAWLLFVPGVGLVCTQVLGHAITSHEYLGVASMLSGFFYGYTLCIMPTIVGDAFGIANFSGNWGLVGLAPVVPSFVLTNLFGKVYDSKSAAGVDGVYTCTLGSRCYSLVFNLSLGVSILALLVVGVLNFGDRYYTSRALATKGKLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDF3</accession>
    <name>A0A1A0HDF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_143272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712454.1">
        <property type="nucleotide sequence ID" value="XM_018854496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDF3"/>
    <dbReference type="GeneID" id="30027472"/>
    <dbReference type="OrthoDB" id="202195at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031333">
        <property type="term" value="P:negative regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010530">
        <property type="entry name" value="B12D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06522">
        <property type="entry name" value="B12D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="81" mass="8996" checksum="BA47750E5EB85872" modified="2016-10-05" version="1">MRSSQILRLAAKKSTAPKVGAPVEMYPLFAAVGVALVSLTYFTYRHFAYDKHLRLWKNPNLSTLDEVLNKAAEDEKTGGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDL2</accession>
    <name>A0A1A0HDL2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_10960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712561.1">
        <property type="nucleotide sequence ID" value="XM_018853887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDL2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDL2"/>
    <dbReference type="GeneID" id="30026863"/>
    <dbReference type="OrthoDB" id="10057496at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188">
        <property type="entry name" value="ANKYRIN REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24188:SF29">
        <property type="entry name" value="GH09064P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="21632" checksum="F28E45F4AE62FB91" modified="2016-10-05" version="1">MATSLSQEEQDAIIYDAREGDLDYLREVFSQIIPGSFLPSIRDEITLSTPVHMAAANGHLAVVQYLLSLVPHAQAVDLANAKNDTGNTPLHWAAYNGHLDIVKLLVQKYAADVYAKNLASHDALFEAERNGQTAVENWILREFAPEETIQVDDLGPDTKITYTPGLESHDIDRQAAAALEAAQKVADVEKLTKNLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE89</accession>
    <name>A0A1A0HE89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Damage-regulated import facilitator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31224</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DIF1/spd1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712801.1">
        <property type="nucleotide sequence ID" value="XM_018855813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE89"/>
    <dbReference type="GeneID" id="30028789"/>
    <dbReference type="OrthoDB" id="4072855at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013900">
        <property type="entry name" value="RNR_inhibitor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08591">
        <property type="entry name" value="RNR_inhib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="36"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="166" mass="18392" checksum="794ACA77F7E2D037" modified="2016-10-05" version="1">MENPHTKRQMHQAPTPENADPYAASLMTLGMRIRKAVADGHPGNRQYSYKPQFGGESVESQPRNQPERTPLPAHLSQPPALTSDGSTFQSGLNVCEWGAPSVNVQTLPLMGTKRKHDEEPDETECSHFEQHGGHHGYHAGSARVSSFGSWEEFSQKNGALHFDEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEG2</accession>
    <name>A0A1A0HEG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31284</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712871.1">
        <property type="nucleotide sequence ID" value="XM_018855826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEG2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEG2"/>
    <dbReference type="GeneID" id="30028802"/>
    <dbReference type="OrthoDB" id="6730379at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF40">
        <property type="entry name" value="THIAMINE PATHWAY TRANSPORTER THI73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="449"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="59"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="505" mass="55831" checksum="38D6E102087EF1D1" modified="2016-10-05" version="1">MLSQKTPYVTDVQSLESSTPEAITYIGNKNLDYIQDFDHEAHPEEVSSDTVIRKMDKVLVFPMLVVYLLQFLDKVVLNYSNVMGMPTDLNFTGNQFSDLATFFFVAYIVGEPFQAFLLQKFAVHYVLGGNVIVWGIATLCCAAVQNYHGMLALRVILGFAEAAVVPCLILLTTNFYDRSKAAFRIGIFYSGLGFGQIFGGLLSFLFQLVDSTTFEGWRILFLVVGIINILTGLYVVVFIPPEPLKAKMLSPKEKFVLLETLTETKIGVVSHKFKPRQVVEMMLDPQCWLYLVISATISFSSNTISSFSAKDIVSFGFSAKQAALLNMPSGVVSIVTSWISTYFIMRGTTRYVAICVLLVPAICGAALMSFLPKSNKAGLLIGIYMINCITAPLAIVYSWASANVAGSTKRIGVTSLYISIGFALGNITGPQSYRAEDAPYYYPAKISMLATQAASFGLALVIAGIYYLRNKRRDSMALKENVEGLVLDVWSDLTDMQNKAFRYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEG5</accession>
    <name>A0A1A0HEG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77882</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713000.1">
        <property type="nucleotide sequence ID" value="XM_018858785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEG5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEG5"/>
    <dbReference type="GeneID" id="30031761"/>
    <dbReference type="OrthoDB" id="30343at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040107">
        <property type="entry name" value="Snu23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45986:SF1">
        <property type="entry name" value="ZINC FINGER MATRIN-TYPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="180"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="184"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="222" mass="24747" checksum="1289E72067F6D6E8" modified="2016-10-05" version="1">MADKTSTDQYGRKKWDVDAYEADAKSGKKTTTTQPSDEAWARKLQKQNFLEHRADLLDQAIDSVGKHTLIGSENATSLTYGKNKRFGFSCPTCDLSFRDTLALVDHFNSPQHLHNVRKVQGGDLGMGEELENGVKRASVDQVIATIEDLVKKLLREKAADSNGHVALSFQERVRRRLEFEQQKSSRRLAKKKRARATAAGEVEQTEMGKLMGFEGFGSTKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF24</accession>
    <name>A0A1A0HF24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Adenosine kinase</fullName>
            <shortName evidence="11">AK</shortName>
            <ecNumber evidence="4 11">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_39434</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="4 11"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712984.1">
        <property type="nucleotide sequence ID" value="XM_018857141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF24"/>
    <dbReference type="STRING" id="869754.A0A1A0HF24"/>
    <dbReference type="GeneID" id="30030117"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00659"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000014">
        <property type="entry name" value="ADO1p Adenosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="12">
        <location>
            <begin position="22"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="346" mass="37189" checksum="BC0A9C4BDC4782C4" modified="2016-10-05" version="1">MSYPLVCLGNPLLDLQVDVDAAYLAKYDLKADDAILIEEKHKPIYDEVLKMDGLKLVAGGAAQNTARGAQYLLPEGSVAYFGSVGNDVYAEKLNEANAQYGLKTLYQVQENTATGKCAALITGKHRSLVTDLAAANEFTPSHLQKPENWAFVENAKYFYIGGFHLTVSPEAIQLLGKHAADNNKTFALNFSAPFIAQFFKDALDASVPYCDYIVANESEAAAYAESHGLDTTDVTEIAKHVAKLPKANTNKPRTVVFTQGTEPTVTVTYHAGTDSFDVNSYAVRELPQEKVVDTNGAGDAFAAGFIAALVEGKALAEAVSVGQWAASISIQEVGPTFPFPKQTYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF46</accession>
    <name>A0A1A0HF46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nuclear mRNA export factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_39990</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713004.1">
        <property type="nucleotide sequence ID" value="XM_018857215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF46"/>
    <dbReference type="STRING" id="869754.A0A1A0HF46"/>
    <dbReference type="GeneID" id="30030191"/>
    <dbReference type="OrthoDB" id="264795at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017173">
        <property type="entry name" value="Sac3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024293">
        <property type="entry name" value="SAC3_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12209">
        <property type="entry name" value="SAC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037320">
        <property type="entry name" value="mRNA_export_factor_Sac3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="3">
        <location>
            <begin position="134"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="SAC3 helical" evidence="4">
        <location>
            <begin position="712"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="626"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1189"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="548"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1204"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR037320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1213" mass="137557" checksum="39F56600A3D4CB95" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFB2</accession>
    <name>A0A1A0HFB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37444</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713310.1">
        <property type="nucleotide sequence ID" value="XM_018856872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFB2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFB2"/>
    <dbReference type="GeneID" id="30029848"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="10">
        <location>
            <begin position="62"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="9">
        <location>
            <begin position="146"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="8">
        <location>
            <begin position="269"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="443" mass="49375" checksum="D8DC6CE89F648773" modified="2016-10-05" version="1">MSVADEIPAIFLDKISPRALETIQKCKDFVNDYCLPADTIYHGQVSEDPQKRWATTPPVLDRLKEKARLLGLWNLFLSKQYAEGGGYTNLEYGIMAEYLGRSFTAPMATNMASPDTGNMEILARYGTAEQKRTWLQPLLDGQIRSAFLMTEKGTSSSNALNIACSARLNGRGNYVLNGVKWFASGAGDPHCSVWLVMCKTGDHKDNVYRNHSVLVVDAKRALATGKARLVRPLSVFGYDDAPHGHCEVVFEDFEIPDDARDHAVLHREGAGFEVIQSRLGPGRIHHCMRCIGVGEIALMRVVHRANHRLIFGRPLKDRESFLTAYAQFKIDIERCRLLVLNAAHKIDVGGPKSAMREIAVAKIETPRTILKILDWGIQMYGAEGVSQDTELARSYALNRTLRIADGPDEAHLGQLARTAAKRFSEADDFFERVESQRAVVAKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFP0</accession>
    <name>A0A1A0HFP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vacuolar protein sorting-associated protein 35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34528</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in vesicular protein sorting.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the VPS35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713154.1">
        <property type="nucleotide sequence ID" value="XM_018856482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFP0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFP0"/>
    <dbReference type="GeneID" id="30029458"/>
    <dbReference type="OrthoDB" id="10258141at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170071">
        <property type="term" value="C:CROP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030906">
        <property type="term" value="C:retromer, cargo-selective complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140312">
        <property type="term" value="F:cargo adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900102">
        <property type="term" value="P:negative regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.660">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 35, helical subcomplex Vps35-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005378">
        <property type="entry name" value="Vps35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042491">
        <property type="entry name" value="Vps35_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11099">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03635">
        <property type="entry name" value="Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009375">
        <property type="entry name" value="Retromer_Vps35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="870" mass="99894" checksum="D47D8DC1F56FBCBD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFS4</accession>
    <name>A0A1A0HFS4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Redoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_29553</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713490.1">
        <property type="nucleotide sequence ID" value="XM_018855435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFS4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFS4"/>
    <dbReference type="GeneID" id="30028411"/>
    <dbReference type="OrthoDB" id="1882547at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000159">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF39">
        <property type="entry name" value="PEROXISOMAL MEMBRANE ASSOCIATED PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 7" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="24"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="19474" checksum="D9F7E7701E4CEFC9" modified="2016-10-05" version="1">MFALRNRLAPQLARSFSSSSSRLVAAGDKIPSVSLFEGSPGNEVNLAEEAASGKAVIVGVPGAFSPACLASHIPGFLSHLSAFQDKGYKSIYVTGVNDAFVFKAWGEAINAKGTEGVRFLADPKGEFAKKWDVLFDASKFFGNERSERYAVLVDNGKVTHAFVEPEKTSIDVSAAEEVLKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFU7</accession>
    <name>A0A1A0HFU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Imidazoleglycerol-phosphate dehydratase</fullName>
            <ecNumber evidence="4 9">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_36212</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="4 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713357.1">
        <property type="nucleotide sequence ID" value="XM_018856709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFU7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFU7"/>
    <dbReference type="GeneID" id="30029685"/>
    <dbReference type="OrthoDB" id="447729at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000005">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="224" mass="24176" checksum="714EA20C5A9394A8" modified="2016-10-05" version="1">MSSLSTRRSAFLARDTNETKIQIALSLDGGHIAVEKSILTQQTFEEHAVQQTLSQVINVQTGIGFLDHMLHALAKHSGWSLIVECIGDLHIDDHHTAEDVGISLGLAFHKALGQVKGVKRFGSGFAPLDEALSRAVVDLSNRPYAVIELGLKREKIGDLSCEMIPHVLESFAQGAAITIHVDCLRGSNDHHRAESAFKALAVAIKEAISLNGTNEVPSTKGVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG21</accession>
    <name>A0A1A0HG21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_75976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713284.1">
        <property type="nucleotide sequence ID" value="XM_018858457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG21"/>
    <dbReference type="STRING" id="869754.A0A1A0HG21"/>
    <dbReference type="GeneID" id="30031433"/>
    <dbReference type="OrthoDB" id="427480at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13969">
        <property type="entry name" value="ADCK1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045307">
        <property type="entry name" value="ADCK1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051130">
        <property type="entry name" value="Mito_struct-func_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173:SF19">
        <property type="entry name" value="AARF DOMAIN-CONTAINING PROTEIN KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43173">
        <property type="entry name" value="ABC1 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="2">
        <location>
            <begin position="95"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="508" mass="57024" checksum="D4B02359A537ED13" modified="2016-10-05" version="1">MHDGARHVYDAARRVSVVAVATARCFQIYRHVLGADFGGDAAGRHAALAEAHLAAARVALRALEENGGIYIKLGQHVSALTYLLPPQWTETMTPLQDRCPRLSLAEIELMFRQDMGVPLAEYFSDFDPQPVGVASLAQVHIATLRATGERVAVKVQHPLLARFVPLDVYMTRTVFGLMNRVFPEYPMAWLADELQNSIYVELDFTNEAANAQRTAAQFAPLRRRTALRVPAVVSAEKRVLVMEYVAGARLDDLAYMEKHGIRTRDVLACLSHVFNSMIFSPGFGLHCDPHLGNLAIRAADPRSSTTGRNFEIVLYDHGLYRYIPLQMQRDYAHFWLAILDNDVPRMKHYVEKFSGVLGDQKFRIFASAITGRDPDSVLNYDISKARDDKELLNIQTQLNTPEVLEDLMAILASMPRMVLLILKTNDLTRNLDETLNHPLGPERTFLILANYCASVVYREAVDAVGRASAAYSWARMKGTVAAWLAYRKRLCSLYFLDAAVFVRNIVRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG28</accession>
    <name>A0A1A0HG28_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FAD/NAD(P)-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_76091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713421.1">
        <property type="nucleotide sequence ID" value="XM_018858475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG28"/>
    <dbReference type="STRING" id="869754.A0A1A0HG28"/>
    <dbReference type="GeneID" id="30031451"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="420" mass="47731" checksum="E24620D9D0D4447D" modified="2016-10-05" version="1">MHYSVIIIGGGMAAGQSNTLILEVKDRLGGRLKSHNSTLKPGVCYDLGALWFHDSLRNPLLEKAKKLGNVEYFYDDGKHIYASEHYRSVPAWEYERVMADIETFGFHGGELISCQAQVSQQVLRMWLEMWDGILWEVASAKHFFSWGADHLGRNAHDKNGFETVFRNELAELPEAYQKQNIRLNTQVTHINYENNDYVTLTTSEGSVYTSDYVVVTAPLSVLRITDQNDRHILNWAPSLPQRFQNFLPECHYGSLGKVVLEFEKCFWPTDVHRFYILASEMSSLDIVRPWQNPTLIINYFAIAKVPSLVFLTQEPVSTQVERWSGKQIWQLFEPVVKQIATGPISEPFEILKSDWNNDPWTQGSYTAGRINTRLADEGCLRFAGAETIGGSSNGCAHGAFLSGEREARFILLQEKVLSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG90</accession>
    <name>A0A1A0HG90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein NSA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_76240</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the pre-66S ribosomal particle.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NSA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713660.1">
        <property type="nucleotide sequence ID" value="XM_018858500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG90"/>
    <dbReference type="STRING" id="869754.A0A1A0HG90"/>
    <dbReference type="GeneID" id="30031476"/>
    <dbReference type="OrthoDB" id="18388at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22858">
        <property type="entry name" value="Nsa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037379">
        <property type="entry name" value="WDR74/Nsa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038">
        <property type="entry name" value="NOP SEVEN ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16038:SF4">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="353"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="353"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="397" mass="44190" checksum="E403EE5CEBDC0A42" modified="2016-10-05" version="1">MRFFVSADESGNVKEVVCSKGTDTSKKDGQQPDLVQNLLQKHDLTTVKTRIVHLKTFQERWIVTARLGGFLNPVSLVAFEEHDFVLVAFESAKLFVVHFDGGKFTTAPLCIALPPRQTKNPTTLSAFVANPYVAGVFAYGGKDNDLQVIRLFDSSKKFSNADFADLKSWKVKTLFQAENVEPDHLDLEVPIWITNILFMQGEAKKKFKLVTATKYGHIRKYNTAEDTEPTELYKVCDKPIISMVFASENQDEVIISDTHTFVAKLSLTQVDKKAHRIISASAGTFYKPSLKLLGKFSEGGNTGAIYGVDVSFNAGLVAIGGLDRYLRVFDIGSRKLVLKVYLGTQISTIKMLDDTDSGDKEGDKRDAEEDDEFWNALDEGKDTNGGAPLLKKRKRNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGF3</accession>
    <name>A0A1A0HGF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase II degradation factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_9277</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Recruits the ubiquitination machinery to RNA polymerase II for polyubiquitination, removal and degradation, when the transcription-coupled repair (TCR) factor RAD26 fails to efficiently displace stalled RNA polymerase II. Also involved in telomere length regulation. Binds DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DEF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713414.1">
        <property type="nucleotide sequence ID" value="XM_018859348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGF3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGF3"/>
    <dbReference type="GeneID" id="30032323"/>
    <dbReference type="OrthoDB" id="5396806at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14368">
        <property type="entry name" value="CUE_DEF1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041803">
        <property type="entry name" value="DEF1_CUE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0779">Telomere</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="17">
        <location>
            <begin position="25"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="69"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="555"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="604"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="140"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="367"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="427"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="614"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="634"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA22933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="683" mass="73340" checksum="10108F727A2B3BC7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGH1</accession>
    <name>A0A1A0HGH1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 16">Galactose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="4 16">2.7.7.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GAL7</name>
        <name evidence="19" type="ORF">METBIDRAFT_35015</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>alpha-D-galactose 1-phosphate + UDP-alpha-D-glucose = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:13989"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="2.7.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="15">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.12" evidence="4 16"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713571.1">
        <property type="nucleotide sequence ID" value="XM_018856554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGH1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGH1"/>
    <dbReference type="GeneID" id="30029530"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008108">
        <property type="term" value="F:UDP-glucose:hexose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052574">
        <property type="term" value="P:UDP-galactose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00608">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000001">
        <property type="entry name" value="Galactose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001937">
        <property type="entry name" value="GalP_UDPtransf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019779">
        <property type="entry name" value="GalP_UDPtransf1_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005850">
        <property type="entry name" value="GalP_Utransf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005849">
        <property type="entry name" value="GalP_Utransf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00209">
        <property type="entry name" value="galT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008724">
        <property type="entry name" value="PRK11720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11943:SF1">
        <property type="entry name" value="GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02744">
        <property type="entry name" value="GalP_UDP_tr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01087">
        <property type="entry name" value="GalP_UDP_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000808">
        <property type="entry name" value="GalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00117">
        <property type="entry name" value="GAL_P_UDP_TRANSF_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10 16" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 16 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase N-terminal" evidence="17">
        <location>
            <begin position="4"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose-1-phosphate uridyl transferase C-terminal" evidence="18">
        <location>
            <begin position="202"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-UMP-histidine intermediate" evidence="12">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="331"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="336"/>
            <end position="337"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>UDP-alpha-D-glucose</name>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000808-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA23090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="371" mass="41921" checksum="9D504ACFD38181A2" modified="2016-10-05" version="1">MAAFSFSEHSHRRYNPLTDDFVLCSPHRAKRPWQGAEETVAKNELPTYDPKCYLCPGNSRASGGVANPKYESTYVFPNDFPAVQLDQPEYEDGEQLDLAVSQLKQKLLRVSSVRGKCYVICFSPNHALTLPLMTHSEITLVVDTWQSLYFETKSQAEKGEAPFTYLQIFENKGLAMGCSNPHPHGQAWCLDVVPTEVSHELANMAKYVERNNSHLLADYVELELAARERIVTENDSFLVVVPFWAVWPFETLLLAKTHLPSLAEFSEKHKADLAAALKTLTTKYDNLFKTSFPYSMGLHQAPLNATDAEKKSSWFHMHFYPPLLRSATVKKFLVGFEMLGEAQRDLTSEQAATRLSELDGVVHYTKDLHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGM4</accession>
    <name>A0A1A0HGM4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_96188</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713484.1">
        <property type="nucleotide sequence ID" value="XM_018859424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGM4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGM4"/>
    <dbReference type="GeneID" id="30032399"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="125" mass="14034" checksum="3FA148195F62694D" modified="2016-10-05" version="1">MYHSGLAIENTQTVYLSTSTSVLQVSGLQNLFNVVEVKVAGFGNSNAIDLDICFLPQATTFEYLEDTGTLEVTHFRRLVIRLQIGPRYVSRHFRLTRGLYGSRIIYHLPAPHYPPDSCSCEPVCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH26</accession>
    <name>A0A1A0HH26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit tim16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 13">Presequence translocated-associated motor subunit PAM16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_23644</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to regulate activity of mtHSP70 (SSC1) via its interaction with PAM18/TIM14. May act by positioning PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize ATPase stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713782.1">
        <property type="nucleotide sequence ID" value="XM_018855264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH26"/>
    <dbReference type="STRING" id="869754.A0A1A0HH26"/>
    <dbReference type="GeneID" id="30028240"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
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    <sequence length="113" mass="12563" checksum="49BF026E9FE770C1" modified="2016-10-05" version="1" fragment="single">MAHRLLVNVVLTGATVFGRAFTEAYKQAAKASATAQATGATKAKSAGGIPLDEAMKILDIDRKNLSMEEIDKKYDYLFDVNSKDKGNSFYLQSKVYYAMDALKKELEYLQKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH93</accession>
    <name>A0A1A0HH93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin cytoskeleton-regulatory complex protein PAN1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Actin cytoskeleton-regulatory complex protein pan1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_63645</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the PAN1 actin cytoskeleton-regulatory complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Actin patch</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PAN1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018858335.1"/>
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            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA23247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1397" mass="150942" checksum="714799A46687F6C0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH96</accession>
    <name>A0A1A0HH96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_9807</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714035.1">
        <property type="nucleotide sequence ID" value="XM_018859462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH96"/>
    <dbReference type="STRING" id="869754.A0A1A0HH96"/>
    <dbReference type="GeneID" id="30032437"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="77"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="31704" checksum="8B94CECDCC220866" modified="2016-10-05" version="1">MALPKLAEELYALFDSENYEKCQKLLPPIKIELIKHNLLVPMGANTETTEQLNDLKIAERILEIGALLLLLAAEYQAFEHYFASLRPFYGSARLHAQREANTDSTKIISLFLIYLLSQGHISKYHVELELIYSSPLIDVEKDNFLQYPIDLERNLMEGNYIKIWKLLQDDADLPCKEYRHFTSSLRNTLRVEIAHSLEKTGDSIPVSNCKTLLYYPQEQSDLLFEAALREELDVTSWTFKDGFVYFEIGKNNALASDNAAMIKNVLSYAEQIESIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHE3</accession>
    <name>A0A1A0HHE3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_37344</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713902.1">
        <property type="nucleotide sequence ID" value="XM_018856862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHE3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHE3"/>
    <dbReference type="GeneID" id="30029838"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032297">
        <property type="term" value="P:negative regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="190"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="362"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="505"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="540"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="684" mass="77594" checksum="BE10D354E39BDA2B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHP5</accession>
    <name>A0A1A0HHP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="13">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_37870</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713883.1">
        <property type="nucleotide sequence ID" value="XM_018856923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHP5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHP5"/>
    <dbReference type="GeneID" id="30029899"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF55">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSYL-OLIGOSACCHARIDE 1,2-ALPHA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="578"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="611"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="629"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="546"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="370"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA23402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="650" mass="74130" checksum="A84267BDBEEB5564" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHT9</accession>
    <name>A0A1A0HHT9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_37748</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the complex I NDUFS4 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714056.1">
        <property type="nucleotide sequence ID" value="XM_018856909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHT9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHT9"/>
    <dbReference type="GeneID" id="30029885"/>
    <dbReference type="OrthoDB" id="3089at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.190:FF:000001">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 21 kDa subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.190">
        <property type="entry name" value="atu1810 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006885">
        <property type="entry name" value="NADH_UbQ_FeS_4_mit-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038532">
        <property type="entry name" value="NDUFS4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219:SF8">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12219">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04800">
        <property type="entry name" value="NDUS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5 9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="156" mass="18028" checksum="B706DCC7686741CA" modified="2016-10-05" version="1">MLTRSILRVAVPVRQLSTTAMLRQQPALYDEAQYKPNEIISGAPKDMTTERVVRIFKESKPATQSGNHNGKFWKLDWDVLGKGNRWENDLMGYQGSADYMQGTIMKFDTKDSAIKFAEGQGWSFYVQEPKKRHFRKKEYAMNFAHSPGPLKHIRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHX8</accession>
    <name>A0A1A0HHX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_29911</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713929.1">
        <property type="nucleotide sequence ID" value="XM_018855511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHX8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHX8"/>
    <dbReference type="GeneID" id="30028487"/>
    <dbReference type="OrthoDB" id="27226at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000143">
        <property type="entry name" value="Phosphoserine phosphatase serb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="302" mass="33406" checksum="F8CD7E060856FD25" modified="2016-10-05" version="1">MYSLTFIANGPRLSEENNRDIEEFVTTRLQVSVLTKTQLSANRVIDFQIDTNVPELVSALVKKEAASKTYDIIFQLASERKNKKLFIFDMDSTLIYQEVIELIAAYADIEDKVAEITERAMNGELDFNDSLRERVLLLKGIDASTLWDELKDKIKITKGARELCKALKKLGVVMGVCSGGFIPLASYVKEQLGLDYAFANTLGVDEQSKLDGTTRGYIVNGEKKAELLLEIASNHNIDPKTAVAVGDGANDLKMMSTGGWGVAWNAKPKVQKEAPSCLNSDSLQDILYIMGFYDSEIAALLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI12</accession>
    <name>A0A1A0HI12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_76625</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714122.1">
        <property type="nucleotide sequence ID" value="XM_018858563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI12"/>
    <dbReference type="STRING" id="869754.A0A1A0HI12"/>
    <dbReference type="GeneID" id="30031539"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071618">
        <property type="term" value="F:lysophosphatidylethanolamine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007114">
        <property type="term" value="P:cell budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07229">
        <property type="entry name" value="Pat_TGL3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF44">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="200"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="618" mass="71124" checksum="F1E234162960D16D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI32</accession>
    <name>A0A1A0HI32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pyridoxal kinase</fullName>
            <ecNumber evidence="2">2.7.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_38446</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714142.1">
        <property type="nucleotide sequence ID" value="XM_018857002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI32"/>
    <dbReference type="STRING" id="869754.A0A1A0HI32"/>
    <dbReference type="GeneID" id="30029978"/>
    <dbReference type="OrthoDB" id="2104723at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534:SF2">
        <property type="entry name" value="PYRIDOXAL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="7">
        <location>
            <begin position="105"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="33742" checksum="D6755E9329045D95" modified="2016-10-05" version="1">MQKNALSILSHVAHGYVGNRAVVFTLQYCGWNVDALHTTEFLNHPGYGQFKGQKADVETVKLIFEGLQSILDLRLEYELILVGYCPSAEVMSTLYEQITPLMGAAKEKSRPILVVDPVLGDNGKLYVPQQIVKAHIEFLQLGFVDLTTPNQFELELLTGIQIHDWESVKLALTTFNEKYKVPNVVILSVIVDGRMYGVGFSKATEAIFYIPIAKIDCRFSGCGDVFTALLTNIFYENNCTLTPKVLSQVLIKLNRILMISFNEEKAKLGKAPIVVQDVRLIQLRKVLDENFLHDLEVSYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIG7</accession>
    <name>A0A1A0HIG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_109282</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714114.1">
        <property type="nucleotide sequence ID" value="XM_018853884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIG7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIG7"/>
    <dbReference type="GeneID" id="30026860"/>
    <dbReference type="OrthoDB" id="418412at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="329" mass="37438" checksum="76743C9401BEC646" modified="2016-10-05" version="1">MSWSAGAFGGIRPMAPWIWVVLNGSERAPADSGAVPHTRVWRHSTSSQALVLPIYPCLYIMPISMPIYPCLCTHAYISCLYPCLYIHAYVPLPIYPRLCTLAYISMPMYPCLYIHAYISTPIYPCLYIHAYISLPIYPCLYIHAYISMPISMPIYPCLCTHAYISTPMYPCLYIHAYISLPISMPIYPCLCTHAYISTPIYPRLYIHAYISTTIYPCLYPCLYIHAYVPMPIYPRLYILAYISTPIYPCLYPCLYMDGVRAGTSATYTRNQCNCTMQSDEPMAPPPRPPIHAVNSCMSVLCTCAVYLCCAPGQYIWLRNMQIRTGEIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIQ5</accession>
    <name>A0A1A0HIQ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_116672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714520.1">
        <property type="nucleotide sequence ID" value="XM_018854051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIQ5"/>
    <dbReference type="GeneID" id="30027027"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="460" mass="51135" checksum="408AB54ACC1106CB" modified="2016-10-05" version="1">MRTSINFAAVFQHASIPRTTVVIHNLELTLIYFSSYQLHLLASKATVMTRQTLKRFGLPTWLLHIIGLGPIVYYVISLLETDPTQVASKTLNLIRSSRNQAFKKKMAFIDIGNHGVRGNPSSAGTPHGSAISTFMLTTKMMNRGTMFRARNKHSFELLKEKSEACSSPFVRVSATTANASVKKSDGVVSSSSNTDNACTAKEFSRFLQKSLVKQIEKDLTSGFEIKTSLSEQRVVGSEEVGTEVFDMRNPFLILAVIDEEIFQRPLVRRNRKVRKHKALSLDSSLMLDLTHEINDSESEGAVDLESSESLKSEPSTDFATPPTDFSLLGEKKERKSEIALMIREIRNKLNSYGSNDQGLKLEAAMADSPFTLKNAGLAPAIAKSGSRKYCSSNSTKIAPLRYRVTSQTAKWGAQTRRHSRSVPHHLKAEWELKWSPNSAFILGFSKSTSLELKNSEHQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIR8</accession>
    <name>A0A1A0HIR8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_111983</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714214.1">
        <property type="nucleotide sequence ID" value="XM_018853929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIR8"/>
    <dbReference type="GeneID" id="30026905"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="74" mass="8382" checksum="C140B83BA7162F2B" modified="2016-10-05" version="1">MEPVAPGLCIFALFNLVYLPRTKTAFARRESNCVFFFYYWPRAVSLCLIQLWSSCFGGLQSATPIGARCPELEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIV3</accession>
    <name>A0A1A0HIV3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714249.1">
        <property type="nucleotide sequence ID" value="XM_018855571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIV3"/>
    <dbReference type="GeneID" id="30028547"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="55" mass="6638" checksum="D4DF887BFBA3AA56" modified="2016-10-05" version="1">MGIIDDFMDIIDGSCTSYMVHVHHWWFHVHHIWFMYIIGGFMYIIDGFVDINTCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIY4</accession>
    <name>A0A1A0HIY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="23">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">METBIDRAFT_34674</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 23">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:38584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(out) + ATP + H2O = a beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66036"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:22801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:66037"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 22">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="23">Membrane</location>
            <topology evidence="23">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="23"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714456.1">
        <property type="nucleotide sequence ID" value="XM_018856509.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HIY4"/>
    <dbReference type="GeneID" id="30029485"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070867">
        <property type="term" value="C:mating projection tip membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990531">
        <property type="term" value="C:phospholipid-translocating ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140351">
        <property type="term" value="F:glycosylceramide flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090554">
        <property type="term" value="F:phosphatidylcholine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099040">
        <property type="term" value="P:ceramide translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000048">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000108">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 21 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10 22 23" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 21 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 23" id="KW-1278">Translocase</keyword>
    <keyword evidence="6 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="160"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="487"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1126"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1157"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1208"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1247"/>
            <end position="1266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1273"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1308"/>
            <end position="1328"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="25">
        <location>
            <begin position="126"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="26">
        <location>
            <begin position="1094"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="267"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="20">
        <location>
            <position position="603"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="604"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="605"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="735"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="776"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="805"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="841"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="921"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="922"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="923"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1042"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1048"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1068"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1071"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1072"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1072"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="OBA23975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1490" mass="168494" checksum="FBAC63262D20E8ED" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ07</accession>
    <name>A0A1A0HJ07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018855575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ07"/>
    <dbReference type="GeneID" id="30028551"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="69" mass="7699" checksum="B5ABAD91FAF9C137" modified="2016-10-05" version="1">MPDTDHNVYSFRPTMFTQSSLTMFTYSSPIIYFQTRPLIYTQTPSSVPGASPSPPALSWPRTPGSMARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ45</accession>
    <name>A0A1A0HJ45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_30503</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714655.1">
        <property type="nucleotide sequence ID" value="XM_018855650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ45"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ45"/>
    <dbReference type="GeneID" id="30028626"/>
    <dbReference type="OrthoDB" id="4094394at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291823" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="7">
        <location>
            <begin position="17"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="8">
        <location>
            <begin position="23"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="339" mass="36272" checksum="046BC06C2581D034" modified="2016-10-05" version="1">MKLIICAAAVSSLVDALAIRDYSVSINHNTVTKSPNVDYKDFSIAAGKHFAAVNSKKVQLSGNFENEGEFYVTNSGDSRVRMSIIGDTVENRGKFAINSLTAETTPGFFIRARESFVNSGEMYLGDSGAESAVFPITISSANSWYNSGSMVFRRESGPRVPLDLRGSLFDIPSIENYGSICLYNTLWETKTSIIGSGCISVGSGSLLELALDKDQVLQAITLESADSDLKISKFGTSLSAPVIRIAGFGGNNKISFDKVIEGTDKLSYSEETGRLSILDATKTVLLLDIGTGYDAGEFKVTQSESGSTVVYDKAVPQDSRQDGCGCVSEFPNSPRVPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ83</accession>
    <name>A0A1A0HJ83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714556.1">
        <property type="nucleotide sequence ID" value="XM_018853722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ83"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ83"/>
    <dbReference type="GeneID" id="30026698"/>
    <dbReference type="OrthoDB" id="8059989at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="518"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="411"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="913" mass="97391" checksum="6FE8347BDF357CB4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ99</accession>
    <name>A0A1A0HJ99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Required for respiratory growth protein 8, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714443.1">
        <property type="nucleotide sequence ID" value="XM_018856795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ99"/>
    <dbReference type="GeneID" id="30029771"/>
    <dbReference type="OrthoDB" id="4015782at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="25383" checksum="F956D63D13CBA533" modified="2016-10-05" version="1">MSRTYLQHPFFKNPVKWLRGVKKRHSPQLCLDGFYDTTSLNVNPFAKSLLETRLDVSRTRFPLENMVQIIVSESEKHGAYELVPVLQKPTCGQNPASYVINNKSYIKFLSRKRFAPIPLKYKHKSSSVIGAIKPPQDLATDIDSLYRSKIKKMIDELPENISVSSKDPEREILIRPAASDCIIWDDSLPVIYSSLAAKEIVLAYAEQHEVISLLLKLVASQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJV7</accession>
    <name>A0A1A0HJV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_10272</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714579.1">
        <property type="nucleotide sequence ID" value="XM_018853726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJV7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJV7"/>
    <dbReference type="GeneID" id="30026702"/>
    <dbReference type="OrthoDB" id="1747771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051430">
        <property type="entry name" value="Fungal_TF_Env_Response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31944:SF131">
        <property type="entry name" value="HEME-RESPONSIVE ZINC FINGER TRANSCRIPTION FACTOR HAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="20"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="47"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="705" mass="79719" checksum="A2E21E01CD26E940" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX6</accession>
    <name>A0A1A0HJX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_10347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714668.1">
        <property type="nucleotide sequence ID" value="XM_018853746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX6"/>
    <dbReference type="GeneID" id="30026722"/>
    <dbReference type="OrthoDB" id="244495at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF27">
        <property type="entry name" value="PHO85 CYCLIN CLG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="335" mass="36457" checksum="00E4D55D3AACFF07" modified="2016-10-05" version="1">MYLSFYPIGTSGHRASALMGFVPYHNYGAPAYGNPANYAPAPMAPVAAQYAPAPAPAFDGQSRAPAHVGGISAVLDYDAARMAAFMCWCTFGMLGQARTPTPQLEASVVSILHATRLPKLTIVIALEYINQRYAACAHLSMPELDVFVKLVVALVLANKFNDDNTFTNKSWFGASGVKVAVLNALEREWLAAVNWNLNVVHFQSNIETLEECWVSWSHKQAHQPCYFAQPPSAPASDNYGSFSSVPSSPLYDSASVSSMYGYSLPVSASPAKFSQDWGVPGPQNFHYLRPGQYTSYAPQPSIWAYPPTTYPSMPPLMDSAYAGLSNPYYNCLATY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK59</accession>
    <name>A0A1A0HK59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">DNA replication complex GINS protein PSF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_37973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714891.1">
        <property type="nucleotide sequence ID" value="XM_018856935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK59"/>
    <dbReference type="STRING" id="869754.A0A1A0HK59"/>
    <dbReference type="GeneID" id="30029911"/>
    <dbReference type="OrthoDB" id="10251744at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11713">
        <property type="entry name" value="GINS_A_psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="8">
        <location>
            <begin position="77"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="175" mass="19824" checksum="CAF0FBBDE7926ECE" modified="2016-10-05" version="1" fragment="single">SMGYYDIDDILADGEKIPCRFNLLVPGLGYLEGNPGKSIDKDTKLDLPLWLAAVLAVCTILDDADESFVDLLEPDFISPKVLNAIKADPKSVDLHSIMSHFYTLAEKWAAMYNEALLVETAMAMLKERALEIDNFASNNTKHVSSNFLYSLDEFEKKLYRVTYESKKEMRAWTND</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK79</accession>
    <name>A0A1A0HK79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD(+) diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_77048</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-adenosine-phospho-ribonucleoside in mRNA + beta-nicotinamide D-ribonucleotide + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60876"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="Rhea" id="RHEA-COMP:15719"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
            <dbReference type="ChEBI" id="CHEBI:144051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:60877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. NudC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.22" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714689.1">
        <property type="nucleotide sequence ID" value="XM_018858628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK79"/>
    <dbReference type="STRING" id="869754.A0A1A0HK79"/>
    <dbReference type="GeneID" id="30031604"/>
    <dbReference type="OrthoDB" id="10249612at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019677">
        <property type="term" value="P:NAD+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006742">
        <property type="term" value="P:NADP+ catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03429">
        <property type="entry name" value="NUDIX_NADH_pyrophosphatase_Nudt13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050241">
        <property type="entry name" value="NAD-cap_RNA_hydrolase_NudC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049734">
        <property type="entry name" value="NudC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904:SF6">
        <property type="entry name" value="NAD-CAPPED RNA HYDROLASE NUDT12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42904">
        <property type="entry name" value="NUDIX HYDROLASE, NUDC SUBFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="275"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="429" mass="47928" checksum="4B6142F29BF5745A" modified="2016-10-05" version="1">MNQAKLDDPYLPAEVPYFGKEVVNRVSFLREDAEFISSAVLHTSTRFIFYENGNPLVNKARSEGRLVILTNGTDQLATVPDTSEAGTTHGLLDNSAQWKTVLETWAAENKDHAPALRDSGKPSLLFLGLKDESVGLDFHQLKLYEKETYLDHQGRYFGIPYFGVDVSNASGISELVKQHIVLSTPAVNKDDLVYTDSRKHTMAFTMEESALFSHGKMYFDWLGRNRFCPGCGSKVIGIHAGGKLRCTNDNKIALGDEEKYECPVKNTRVSNVSFPRTDAVVITAIVNREKTKMLLSLSKRYAHTKMYSCTAGFMEPSETVEVATRREIWEETGVACSDIQILMTQPWPFPGNLMIGCVATVDFNGLNEVIHLGHDRELADARWFDVSFVRKLIHNTAGDENPDGIVLPTDVSIAYTLIKHAIEKTELKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKN2</accession>
    <name>A0A1A0HKN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 17">Aurora kinase</fullName>
            <ecNumber evidence="2 17">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_77327</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2 17"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715032.1">
        <property type="nucleotide sequence ID" value="XM_018858677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKN2"/>
    <dbReference type="STRING" id="869754.A0A1A0HKN2"/>
    <dbReference type="GeneID" id="30031653"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005828">
        <property type="term" value="C:kinetochore microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045144">
        <property type="term" value="P:meiotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044779">
        <property type="term" value="P:meiotic spindle checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044774">
        <property type="term" value="P:mitotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901925">
        <property type="term" value="P:negative regulation of protein import into nucleus during spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901673">
        <property type="term" value="P:regulation of mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7 17 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="54"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="181"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="16">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA24551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="321" mass="37285" checksum="55FB22B9A99798A9" modified="2016-10-05" version="1">MPLPSRKPLGHISLNTAGRFSRGRYNRKPSTFSQQIILHPNRREERRKLRLADFEIGKPLGKGKLGKVYCARHKASGYICALKVMSKSDLVRHQLENNLRREIEIQRNLVHPYISRLYNFFYDDDNVYLILEYAVHGELYHLLKMYRRFDDVTASNYIYQVSVALQYLHSKGVIHRDIKPENILLSANKTVKLSDFGWSVNSELSGLGRRLTLCGTLDYLPPEMVESKEHDLSVDLWLLGILTYEFLVGRPPFEEVDKTATYKRIAKVDLQIPGFLNAEAASFIKQLLQKDPKNRLTLKDIENHPWMVQNRPYWPKSTSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1U6</accession>
    <name>A0A1A0H1U6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_230703</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709396.1">
        <property type="nucleotide sequence ID" value="XM_018855256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1U6"/>
    <dbReference type="GeneID" id="30028232"/>
    <dbReference type="OrthoDB" id="4086420at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="766" mass="85008" checksum="2B7DDFD32E949312" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H262</accession>
    <name>A0A1A0H262_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Chromatin assembly factor 1 subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_80107</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709407.1">
        <property type="nucleotide sequence ID" value="XM_018859173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H262"/>
    <dbReference type="STRING" id="869754.A0A1A0H262"/>
    <dbReference type="GeneID" id="30032149"/>
    <dbReference type="OrthoDB" id="79480at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048800">
        <property type="entry name" value="Cac1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021644">
        <property type="entry name" value="CAF-1_p150_acidic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022043">
        <property type="entry name" value="CAF1A_DD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272:SF0">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15272">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT A CAF-1 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21796">
        <property type="entry name" value="Cac1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11600">
        <property type="entry name" value="CAF1A_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12253">
        <property type="entry name" value="CAF1A_dimeriz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromatin assembly factor 1 p150 subunit acidic region" evidence="8">
        <location>
            <begin position="87"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit A dimerization" evidence="9">
        <location>
            <begin position="336"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Chromatin assembly factor 1 subunit Cac1-like C-terminal" evidence="10">
        <location>
            <begin position="532"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="107"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="359"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="379"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA18012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="593" mass="67786" checksum="9C5B0B5A09BC3E77" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4M6</accession>
    <name>A0A1A0H4M6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709564.1">
        <property type="nucleotide sequence ID" value="XM_018856365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4M6"/>
    <dbReference type="STRING" id="869754.A0A1A0H4M6"/>
    <dbReference type="GeneID" id="30029341"/>
    <dbReference type="OrthoDB" id="337735at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20558">
        <property type="entry name" value="CYCLIN_ScPCL7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF117">
        <property type="entry name" value="PHO85 CYCLIN PHO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="393" mass="45203" checksum="DB24AC4F2AA9FD1F" modified="2016-10-05" version="1">MSPATRQNSPVQSGHGSPRLKPPANQKHDLDATMTSQQRQERSENSPVIEYEGPPVKSQDFIDPFLSQYIKPTSDLNHEIYFRSLNKQVCIKSFDYYQKYLNHDKGTPNRITSLKHDTTLAQKPKSPLRQEVSPKLESRIEQKPDTLDQRIPQRRRQPGIESGIQPDLKKTQHELISETLLVHFEECPTTDLINLMARMILSLISLNDRSVPASISSPPAFKSPVVGSEQKNKLLTRYHSRTPPAISIHTYISRLAKFNNLTPATLLTTIYYIDLLSHNFQPYFTLNSWTVHRFLLVATMLAQKSLEDFFYTNDHYARVGGVALTELNFLELDFLNRVDWKVVPAKQILSTQTSIKHSKEVLDLYYWQLVNLMGKNITEMDRVTYFLSRGSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4W2</accession>
    <name>A0A1A0H4W2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33586</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709482.1">
        <property type="nucleotide sequence ID" value="XM_018856339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4W2"/>
    <dbReference type="STRING" id="869754.A0A1A0H4W2"/>
    <dbReference type="GeneID" id="30029315"/>
    <dbReference type="OrthoDB" id="440128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010491">
        <property type="entry name" value="PRP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06424">
        <property type="entry name" value="PRP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PRP1 splicing factor N-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="876" mass="99609" checksum="9DC82E73C5A42ADB" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H506</accession>
    <name>A0A1A0H506_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">UDP-N-acetylglucosamine transferase subunit ALG14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Asparagine-linked glycosylation protein 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ALG14</name>
        <name evidence="12" type="ORF">METBIDRAFT_47464</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in protein N-glycosylation. Essential for the second step of the dolichol-linked oligosaccharide pathway. Anchors the catalytic subunit ALG13 to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Heterodimer with ALG13 to form a functional enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ALG14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709527.1">
        <property type="nucleotide sequence ID" value="XM_018858169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H506"/>
    <dbReference type="STRING" id="869754.A0A1A0H506"/>
    <dbReference type="GeneID" id="30031145"/>
    <dbReference type="OrthoDB" id="17098at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004577">
        <property type="term" value="F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013969">
        <property type="entry name" value="Oligosacch_biosynth_Alg14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12154:SF4">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE TRANSFERASE SUBUNIT ALG14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08660">
        <property type="entry name" value="Alg14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA18992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="224" mass="24765" checksum="D187E9B92598FA8C" modified="2016-10-05" version="1">MASENALCAWVICAVVPLCLCLIRVIAVLPALRDGRKQKSHSESDIPVDTSLMIFLGSGGHTGEMLRLLENTDISQCHRTWLVSSGDAASLMKAKKYEESTRSFKNVNYAELHRARKVGESLALSTLSTIKSIFSTVQQLSLLPKPDILLVNGPGTSVPIAYTLFFMSLFGGCKTKIVYIESLARVHNLSLSGRLIMPIANRFLVQWAPLAFKYQRAEYYGILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H543</accession>
    <name>A0A1A0H543_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3 13">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_47277</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Phosphatase that converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'-phosphate (PAP) to AMP. Regulates the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. Involved in salt tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3 13"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709578.1">
        <property type="nucleotide sequence ID" value="XM_018858141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H543"/>
    <dbReference type="STRING" id="869754.A0A1A0H543"/>
    <dbReference type="GeneID" id="30031117"/>
    <dbReference type="OrthoDB" id="411145at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052829">
        <property type="term" value="F:inositol-1,3,4-trisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004441">
        <property type="term" value="F:inositol-1,4-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042538">
        <property type="term" value="P:hyperosmotic salinity response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043647">
        <property type="term" value="P:inositol phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016078">
        <property type="term" value="P:tRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.10:FF:000015">
        <property type="entry name" value="3',5'-bisphosphate nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000003">
        <property type="entry name" value="PAP-specific phosphatase HAL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="361" mass="38963" checksum="4A52D77D2B6D6CFA" modified="2016-10-05" version="1">MSHPYAKELEVATLAVKRASILTKKLSDSISATLKSGTQIKDDKSPVTIGDYAAQAIINHAIKKNFPLDNIVGEEDADSLREDSEEAKSLSSKILEIIQDAQNSSLSSKEIMGSLSDLPEIFDSIDAGNYEGGNKGRFWALDPIDGTKGFLRGDQFAVCLALVEDGEVVLGVIGCPNLAENIISNEEQHGVKGGLFSAVKGLGSFYTPLFKNDKFVPISDQEKINMTQGTSPVDLKVLEGVEKGHSSHSTQSQIKAALGFDEKTVAQQTINLDSQVKYCVLAKGQADIYLRLPINDTYREKIWDHAAGNILLYESGGQVGDITGAKLDFGKGRYLQSKGVIAANSKVFPQVIDAVVKILNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H577</accession>
    <name>A0A1A0H577_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_47237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709597.1">
        <property type="nucleotide sequence ID" value="XM_018858137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H577"/>
    <dbReference type="STRING" id="869754.A0A1A0H577"/>
    <dbReference type="GeneID" id="30031113"/>
    <dbReference type="OrthoDB" id="16547at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010106">
        <property type="term" value="P:cellular response to iron ion starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014842">
        <property type="entry name" value="AFT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08731">
        <property type="entry name" value="AFT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="354"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="49364" checksum="6A4D8FD16B754C0D" modified="2016-10-05" version="1">MSHEEDYATNIDEQLLTAVKNQVNDHELQHNGKAKLEDPEHVLGHEHSHNHNHNQGHRHPQVLYHHQQHLQDVGESEYNIQIPEPIIDSRLKIYPVNENTITNEGNLITRPFPEQLFHGRDELNEFIAEFARDNGFGIVIAHSNRKAIYYTCELGGRYRHKKGKSIEQQEEEAAQSQLNEDGTGYALDSHARTKKLRCPFSMTALYRKSSQIWSLRTTCNEHNHPQLDPLSNHPMLRKRSDELNLLILDLYKVGTKPSHIEAKIKHQFPDVLIKREDIYNEIRGYKRKMKKLNKFYPPGVAPNGTKRRRNAASYHQVMEGDDDDEHDEVLTYELERHRQEADLQRQIQAAEAHHSHHHGISHAHSLSHHSHIDEQQLQHYQHQLQAHGLHDSEHSDTAAAAIAAVANAAHADESGNLSIDNIDSRLVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H590</accession>
    <name>A0A1A0H590_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit A</fullName>
            <shortName evidence="7">eIF3a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 110 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF3 p110</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Translation initiation factor eIF3, p110 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF32</name>
        <name evidence="10" type="ORF">METBIDRAFT_47486</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709623.1">
        <property type="nucleotide sequence ID" value="XM_018858173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H590"/>
    <dbReference type="STRING" id="869754.A0A1A0H590"/>
    <dbReference type="GeneID" id="30031149"/>
    <dbReference type="OrthoDB" id="18884at2759"/>
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        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="330"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="622"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="840"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="840"/>
            <end position="857"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="895" mass="103155" checksum="C75E95575C8DA5E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B9</accession>
    <name>A0A1A0H5B9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 10">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_46720</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 10"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709763.1">
        <property type="nucleotide sequence ID" value="XM_018858067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5B9"/>
    <dbReference type="GeneID" id="30031043"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047938">
        <property type="term" value="F:glucose-6-phosphate 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1390.10:FF:000001">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000004">
        <property type="entry name" value="Glucose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 10" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="553" mass="60755" checksum="E40B59CDB524BB5B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F8</accession>
    <name>A0A1A0H5F8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_33507</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
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        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018856314.1"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="HD"/>
    </dbReference>
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        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF5">
        <property type="entry name" value="HEMATOPOIETICALLY-EXPRESSED HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
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            <end position="63"/>
        </location>
    </feature>
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            <begin position="5"/>
            <end position="64"/>
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        <source>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        </source>
    </evidence>
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            <dbReference type="EMBL" id="OBA19324.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="566" mass="63313" checksum="970C21A0F6B25FB2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5K7</accession>
    <name>A0A1A0H5K7_9ASCO</name>
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        <recommendedName>
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        </recommendedName>
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        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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            <taxon>Metschnikowia</taxon>
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
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                <person name="LaButti K."/>
                <person name="Lapidus A."/>
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                <person name="Peng Y."/>
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                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="1 2">NRRL YB-4993</strain>
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            <dbReference type="EMBL" id="OBA19326.1"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
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        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
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                <person name="Goker M."/>
                <person name="Salamov A."/>
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                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
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                <person name="Peng Y."/>
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                <person name="Scheuner C."/>
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                <person name="Stielow J.B."/>
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                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5N4</accession>
    <name>A0A1A0H5N4_9ASCO</name>
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            <fullName evidence="1">Non-structural maintenance of chromosomes element 1 homolog</fullName>
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        <name evidence="2" type="ORF">METBIDRAFT_13640</name>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
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                <person name="Hanson S.J."/>
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                <person name="Otillar R.P."/>
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                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Smc5-Smc6 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709878.1">
        <property type="nucleotide sequence ID" value="XM_018854401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5N4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5N4"/>
    <dbReference type="GeneID" id="30027377"/>
    <dbReference type="OrthoDB" id="185455at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011513">
        <property type="entry name" value="Nse1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973">
        <property type="entry name" value="NON-SMC ELEMENT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20973:SF0">
        <property type="entry name" value="NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07574">
        <property type="entry name" value="SMC_Nse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="219" mass="24764" checksum="D24393C59924E7AF" modified="2016-10-05" version="1">MYDTTNIPEMSDEFSEAHRVLLTYLRAVKCISADDLLEKFSVLSTHFGIDTSKPMEAMRDYIAEINVQIARAHFKIASIRDQETDIQHYVFINTKSDEVIQACTSYTVPELDTIKHLIDSIVNSHDYAYALPYGNAKQQTTTLLKQKASELDLFIRRLVDDGWINLTDQNRLVLSCASLAELSTYLQDRFGVFSVVLCVTVLLLSERNAATLNAQTLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5P5</accession>
    <name>A0A1A0H5P5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial</fullName>
            <shortName evidence="7">Glu-AdT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">HER2</name>
        <name evidence="9" type="ORF">METBIDRAFT_74707</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709888.1">
        <property type="nucleotide sequence ID" value="XM_018858425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5P5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5P5"/>
    <dbReference type="GeneID" id="30031401"/>
    <dbReference type="OrthoDB" id="421993at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="33"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="50504" checksum="3FFBD0AFEC7EC9E3" modified="2016-10-05" version="1">MIRPSLRRLNEFALDNFNCLVSRRRFRRSLPLSGPLGNTTFAAKDNISTTQEPTTCGSKILETYVSPFQATVITQLEKAGLVLVGKANMDEFGMGSSTTHSSHGPTINPRFTENRICGGSSGGSAAAVAGRIADFALGTDTGGSVRQPASYCGIVGFKPSYGRISRYGVVAYGQGLDCVGILSNNVKTASKVYSVLDEYDEKDITSMPVSTREAVFKNHNENDMSRKLKIGIPQEFILDELHPETRAMLGSVLEDLMDAGHAVYPVSIPSIGKLLSAYYTLATVEAASNLSRFDGLRYGSGARDNDKRADGDTNGQSLIARQRTKYLGPEVQRRIILGNYTLSSEAGDNYLRATQHRSKIVEELNQIFELPNLLWGPSAVSRGTCDVLIGPTAFGKAPTFAEYELQRRDTFLNEYLNDVFTVPASMAGLPAISVPYGKGDCGVQVMGQYGDDETVLRAAELIERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5R2</accession>
    <name>A0A1A0H5R2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Seryl-tRNA synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA(Ser) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_79782</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709785.1">
        <property type="nucleotide sequence ID" value="XM_018859125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5R2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5R2"/>
    <dbReference type="GeneID" id="30032101"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070158">
        <property type="term" value="P:mitochondrial seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="207"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="58"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="295"/>
            <end position="297"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="311"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="382"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="460" mass="51165" checksum="FFC1E12987ADDE62" modified="2016-10-05" version="1">MLWSTIFVRYASSFINTSSLRRPVFDIKGIISRQDEMRNSISRRGDGSSEQLDFLLRNRETELALQDERNLLTSARKKLGFVLGNLLKQNSGGTDLDEIARLQLELLQYKVKISALENKLDVLSETIHNAVESLPNWLSDSVPSDPNVPELQEVINGDSVQQIESALPISKHDHRTIGLNLGLMNFETAARISGSSWYYLINDGALLEQALIQFSLSRARKAGYTMVVPPSIVKSEIISACGFKPRDQNDEKQVYSIEGEPLSLAGTAEIPLGALHSQSTISTPLKYVGVSRSYRAEAGARGKDTAGLYRVHEFTKVELFHFTDSEKAEGELEELREFQASLIRDLGLKAKMLNMPTTDLGAPALKKYDCEAWMPGRGSWGELTSSSNCGDYQARRLGIKQRSKATGKLEYAHTLNGTALAVPRVIVAILEQFYIPETNSVVIPEVLQPFMDDKKEIRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5U4</accession>
    <name>A0A1A0H5U4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Outer arm dynein light chain 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709809.1">
        <property type="nucleotide sequence ID" value="XM_018854392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5U4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5U4"/>
    <dbReference type="GeneID" id="30027368"/>
    <dbReference type="OrthoDB" id="676979at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="403" mass="45545" checksum="42DF46F90469595F" modified="2016-10-05" version="1">MESFLSFPPEIQTHVLCFLSQRELTFLFDLECLFKSLSVQKLTRTNFTGNELGSLEQQALFAMFHRKPLLLSNEFASKSMPLCHLRALIDLGVHITPSEVNIHVHDFTDYDGSFSIWDCFSPSLFQFLLRLSSNINVELVVWEDIPLDPDIMRAWFKPLIEHGIALSSCKIRHLSVFGLASPRPTEVSSEHRIVKDIGTIKPMSLYLNFLGPFEAFRSLASTGLCISLDNIRCLDLSYNQLSDMEFRSLRLPGSLEELNLSNNSMSVFDSESFPFEELPLLKSLDLSNNNIMRLDICQPRADVSSSVKRLNLSGNFLCKYSQLFSGHIFANLEELDLSHNLISQVSTFSMSMKSVNLAGNYITTQPGAYALYFPKSLEILGIDQLLVRDLHEANGMNNAAHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5U9</accession>
    <name>A0A1A0H5U9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13582</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709814.1">
        <property type="nucleotide sequence ID" value="XM_018854393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5U9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5U9"/>
    <dbReference type="GeneID" id="30027369"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="34"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="359" mass="40016" checksum="63B88707E39EC62A" modified="2016-10-05" version="1">MSLWVDKYRPRSLDALTFHDNVTSSLRALASTGDFPHLLVYGPNGAGKKTRIYSTLNELFGAQVEKLKIDVKTFTTSSNRKLEFNVLSSPYHLEITPSDMGNNDRVVIQDLLKDVASTEQVDFSNQAKSASKHRFKVVVINEAESLSRDAQAALRRTMEKYSSNIRLILVCNTISNIIAPIKSRTLLVRIPAPTLTEIADVLRIVVLKESVKLSTSHEGAQNTFFETIAGAADRNLRRALLSFETICMQSETVDVQNVNAAFIVLDWEQIIKNIAVSMRRDKSVANLAKLRVTFYELLSHCIPPRLILKTLALHLVQMEQARTPEIVACAAVFDERLSLGLKAIFHLEGFAAKAMVAMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H617</accession>
    <name>A0A1A0H617_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_47009</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709932.1">
        <property type="nucleotide sequence ID" value="XM_018858113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H617"/>
    <dbReference type="STRING" id="869754.A0A1A0H617"/>
    <dbReference type="GeneID" id="30031089"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004103">
        <property type="term" value="F:choline kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05157">
        <property type="entry name" value="ETNK_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007521">
        <property type="entry name" value="Choline_kin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF93">
        <property type="entry name" value="RE24176P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04428">
        <property type="entry name" value="Choline_kin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Choline kinase N-terminal" evidence="3">
        <location>
            <begin position="66"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="547" mass="62886" checksum="F72D9EF1C4E914D8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6H1</accession>
    <name>A0A1A0H6H1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45884</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710088.1">
        <property type="nucleotide sequence ID" value="XM_018857969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6H1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6H1"/>
    <dbReference type="GeneID" id="30030945"/>
    <dbReference type="OrthoDB" id="4088875at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020999">
        <property type="entry name" value="Chitin_synth_reg_RCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28187:SF1">
        <property type="entry name" value="PROTEIN RCR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12273">
        <property type="entry name" value="RCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="22059" checksum="D66030574FDBB8CE" modified="2016-10-05" version="1">MPNIHSVEHHLNRRYFYASDWSGGARWAFFAIFIAIIVICVIGTIRVNKKRSRQGIQPIYGTRWMTPPSYFQSQDQYNQPARGDADIPTAYVPTYTEQANANDMGYYAPDGTFYPNPNSKGPIFPESAHHRTTSNADGQPLLQFDQNGVRVFSDEMTEDFTRPTGPPSGMTGISRDDTAPSSVDIYERPGHPPPGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6I4</accession>
    <name>A0A1A0H6I4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710045.1">
        <property type="nucleotide sequence ID" value="XM_018859082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6I4"/>
    <dbReference type="GeneID" id="30032058"/>
    <dbReference type="OrthoDB" id="5330253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018809">
        <property type="entry name" value="DUF2406"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28186:SF1">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10295">
        <property type="entry name" value="DUF2406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="183" mass="20696" checksum="CFBE67CF3FFEA5D4" modified="2016-10-05" version="1">MTVFRTDSKSQQPDQQPHHLGQNKLDASKMHDPILSAVNEAQPFEEANQVQFTRKQSYLSQGSDELRDPFGNKIRQADVLNPSRSRNERPLDTIRAFEYAITGDMTYRDQLESDRLGWGFHEDFPHHNLSTLASGSLQTGYSQPVASFSGANQQVYQATNYSALSLKPEPKKKKGLFGPRNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6J8</accession>
    <name>A0A1A0H6J8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_22026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710113.1">
        <property type="nucleotide sequence ID" value="XM_018855133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6J8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6J8"/>
    <dbReference type="GeneID" id="30028109"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291682" evidence="2">
        <location>
            <begin position="20"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="152" mass="16323" checksum="97902C8070C118D4" modified="2016-10-05" version="1" fragment="single">MKFSSAVAAGVLAAGLVSAADETREIVTVTVTGSTDTHVYGRFDKTKDATTSTSSGTHKYGRFDKTKDATTSTSSGTHKYGRFDKTKDATTSTSSGTHRYGRFDKTKDATTSTSSGTHRYGRFDKTKDATTSTSSGTHRYGRFDKTKDPLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6K0</accession>
    <name>A0A1A0H6K0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45826</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710066.1">
        <property type="nucleotide sequence ID" value="XM_018857964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6K0"/>
    <dbReference type="STRING" id="869754.A0A1A0H6K0"/>
    <dbReference type="GeneID" id="30030940"/>
    <dbReference type="OrthoDB" id="422106at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019416">
        <property type="entry name" value="NCBP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16291:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10309">
        <property type="entry name" value="NCBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="292" mass="32438" checksum="371800737DFCB605" modified="2016-10-05" version="1">MEPAPYGGFMTRTIAGENEQKELLARQAAEVDVGGVSETLRPDAVHIRGVDNLSTEEIEAFVDLYLNVESDGPEYRRQDEPVWFRVQWVDDSSANVLFKTHADSLRALQRLSEQWPAVENTAGGPDAGGPAFSAPFLAALVEERRARPYAAAVPYHQHRQQLKELADGQDLFQAKKAELEEQKQSAMDEDGSPVVLHVRQALQSDRKVKNAAAYSRYYLLHGEPDRTRPRVARRGGEARGRGTYARAEDDGEDLFAAKIKSGAEAGLFAEKDLSAEEDLFAARMREQSPGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6M1</accession>
    <name>A0A1A0H6M1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_33215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710085.1">
        <property type="nucleotide sequence ID" value="XM_018856245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6M1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6M1"/>
    <dbReference type="GeneID" id="30029221"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015169">
        <property type="term" value="F:glycerol-3-phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001406">
        <property type="term" value="F:glycerophosphodiester transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000140">
        <property type="entry name" value="Putative MFS phospholipid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0843">Virulence</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="421"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="38"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="495" mass="54179" checksum="D23F7BD9DEE0722A" modified="2016-10-05" version="1">MLDSLSSVGKTSPPGRFQYDAEARLGRYLKRSAFQIFTVLASGFALISDGYQNNVMTMLNTVFAGLYPDEYTSDVKTRVSNASLVGTIFGQVIIGVLADRLNRKQSIIIATCFLVFGTVMCAASHGATINGMFWMLTIFRGVTGFGIGAEYPSCSVSANEAANEAFKRRGGVFVLVTNLPLSFGGPFALIIFLIVWQITGLSDALWRTLFAIGAFWPLSVFYFRFKMATSELFKKSAIRKDVPYWLSLKYYWKRLLGTCLCWFLYDFVTYPNGIFSATIISSVLPGSEGSDLEKVAEWNLLLGMIAIPGVLVGAFLCDKLGRRNTLMTGFAGYIVFGLIIGLAFDKVSNITPLFIVFYGLMMSMGNLGPGDMMGLVSSESFATPIRGTLYGFSAAIGKVGAVVGTKTFVPIQDNLGKKWTFIIAAICGLAGVLVAFFFIPQLKDDDLLEEDVRFKNYLVAQGWKGTFGAEEEVLEVTSSAEEGVVEEIVDFQTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6P2</accession>
    <name>A0A1A0H6P2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79129</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetate uptake transporter (AceTr) (TC 2.A.96) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710284.1">
        <property type="nucleotide sequence ID" value="XM_018859006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6P2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6P2"/>
    <dbReference type="GeneID" id="30031982"/>
    <dbReference type="OrthoDB" id="3648309at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015123">
        <property type="term" value="F:acetate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051633">
        <property type="entry name" value="AceTr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000791">
        <property type="entry name" value="Gpr1/Fun34/SatP-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF038013">
        <property type="entry name" value="AceTr_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31123:SF1">
        <property type="entry name" value="ACCUMULATION OF DYADS PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01184">
        <property type="entry name" value="Gpr1_Fun34_YaaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="255" mass="27255" checksum="53F36DC1B142DFE0" modified="2016-10-05" version="1">MSSISGDSHKEVQSDPGVMKISTSGTADQYVTIGKHTYHKHELMEAFGGSLNPGYAPFPTREFGNPSPLGLCGFALTTFVLSMINAQAMGIKVSNVVVGLACFYGGFAQFCAGIWEGFVGNTFAMCALTSYGGFWLSFAAINIEAFGIASAYEDHEGMLADAIGFFLVGWAIFTFMLTLCTLKSTVMFCSLFSFLTMTFVLLAAGEFTGKVGVTRAGGVFGVITAFIAFYNAFAGTANSKNSYITAKPIRLPWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6R1</accession>
    <name>A0A1A0H6R1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_32790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the purine-cytosine permease (2.A.39) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710241.1">
        <property type="nucleotide sequence ID" value="XM_018856129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6R1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6R1"/>
    <dbReference type="GeneID" id="30029105"/>
    <dbReference type="OrthoDB" id="5428495at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4160.10">
        <property type="entry name" value="Hydantoin permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001248">
        <property type="entry name" value="Pur-cyt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026030">
        <property type="entry name" value="Pur-cyt_permease_Fcy2/21/22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806">
        <property type="entry name" value="PURINE-CYTOSINE PERMEASE FCY2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31806:SF17">
        <property type="entry name" value="VITAMIN B6 TRANSPORTER TPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02133">
        <property type="entry name" value="Transp_cyt_pur"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002744">
        <property type="entry name" value="Pur-cyt_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="507"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="533" mass="58535" checksum="A6ABFE3B42BC8B6D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S4</accession>
    <name>A0A1A0H6S4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase 24 kDa subunit mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_45893</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="9">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710136.1">
        <property type="nucleotide sequence ID" value="XM_018857971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S4"/>
    <dbReference type="GeneID" id="30030947"/>
    <dbReference type="OrthoDB" id="10254187at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098796">
        <property type="term" value="C:membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000022">
        <property type="entry name" value="NADH dehydrogenase flavoprotein 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1590:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005725">
        <property type="entry name" value="PRK07539.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10371">
        <property type="entry name" value="NADH DEHYDROGENASE UBIQUINONE FLAVOPROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01257">
        <property type="entry name" value="2Fe-2S_thioredx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="25078" checksum="2AC12DE0271CFA2C" modified="2016-10-05" version="1">MKQASRNSSIISVHRDIKEDNQQLPFEFTAENLSRAKDIIAKYPAQYKKGACMPLLDLGQRQLGFTSISVMNYVAKMLDMPPMRVYEVATFYTMYMRQPMGKYNIQVCTTTPCQLCGSDEVMKAVTDHLKIKPGQTTPDKMFTLQEVECLGACVNAPMMAIDDDFHEDLTAAKTVELLKTLQAGKPLEKRGPVSGRESCEPLSGRKVLLGAEPTDLRKFTRADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H713</accession>
    <name>A0A1A0H713_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinetochore protein mis13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_45762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710216.1">
        <property type="nucleotide sequence ID" value="XM_018857957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H713"/>
    <dbReference type="STRING" id="869754.A0A1A0H713"/>
    <dbReference type="GeneID" id="30030933"/>
    <dbReference type="OrthoDB" id="3364649at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013218">
        <property type="entry name" value="Dsn1/Mis13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14778:SF2">
        <property type="entry name" value="KINETOCHORE-ASSOCIATED PROTEIN DSN1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08202">
        <property type="entry name" value="MIS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="50769" checksum="8F8BA47550FA928B" modified="2016-10-05" version="1">MPPKPPLAARARGARRPRAKKSAPPPAGAAGREAEEAAGSPENRPRHGPSQSLLGPHNTLRQLLAGSAAGPAAAATPPRRAAGALRARLSLSPASSASTPMSRLKHATAQLGILAAGGRLPVRFDDVEAELPPPKKRRPGRPRTQQGRRPPKIEAFPDDDDSFMDHAPPGPPGSALGARPGLSRRSSYNNRGKRGLSIGNGFVAKPHSEVSETDYYKHIDSSMSEPNRMRQLLAWCFRKTMENDKSGPGGASNEARTAEGVAKAIERELLEDIINGAISTSWYSMKDDRPAAQDHVPGRTIIRPNPLNEANKESIAIFSAKLKSLRAEKARLLAAYQASVKGLDDLLLDPGAAPEEALRAYLREHQSDRSVQESTLGEPVPKEVQLHVQKAKSSVELDLELNVDKLHNLLHRLRQSAGLVSKLHQNVLAPEFSDLVRKYAHRGEGKNEPSTRELLRGITKLDRARRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7A6</accession>
    <name>A0A1A0H7A6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Branchpoint-bridging protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_208901</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Necessary for the splicing of pre-mRNA. Has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the BBP/SF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710385.1">
        <property type="nucleotide sequence ID" value="XM_018855021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7A6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7A6"/>
    <dbReference type="GeneID" id="30027997"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045131">
        <property type="term" value="F:pre-mRNA branch point binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048024">
        <property type="term" value="P:regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02395">
        <property type="entry name" value="KH-I_BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032570">
        <property type="entry name" value="SF1-HH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047086">
        <property type="entry name" value="SF1-HH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208:SF45">
        <property type="entry name" value="SPLICING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16275">
        <property type="entry name" value="SF1-HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 13" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="10 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 11 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="15">
        <location>
            <begin position="435"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="500"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="61"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="584"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="628" mass="66797" checksum="8F61D3D72F3E7A83" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7W5</accession>
    <name>A0A1A0H7W5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uridine kinase</fullName>
            <ecNumber evidence="6">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_43881</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the uridine kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710711.1">
        <property type="nucleotide sequence ID" value="XM_018857710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7W5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7W5"/>
    <dbReference type="GeneID" id="30030686"/>
    <dbReference type="OrthoDB" id="738517at2759"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00637"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00579">
        <property type="reaction ID" value="UER00640"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043771">
        <property type="term" value="F:cytidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044211">
        <property type="term" value="P:CTP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000339">
        <property type="entry name" value="Uridine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14681">
        <property type="entry name" value="UPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="7">
        <location>
            <begin position="62"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="8">
        <location>
            <begin position="296"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="509" mass="57031" checksum="E26327012CA30564" modified="2016-10-05" version="1">MSPKAKTHLIRIAPDEQRQASFFTSIKDLKPHSSESSLSADRESQAQLSYDTYVPPWTEPYVIGVAGFSGSGKTSISQQIILELNTPWTVLLLFDNFYKPLSAEESAQAFACNYDFDTPKAIDMDLVVEKLTLLKQGHKTEIPVYSFSHHNRTEKHITIYGANVIIVEGIYALYDQRLLDLMDIKVFVDTDLDVCLARRLTRDILYRGRDLAGALQQWETSVKPNATGSVYPTIRNADLVIPRGLDNTRAIDLMIKHIQKQLALKSAAHLDRLKALGHGVSFDVSRRANLKILPATNHTAGIHSMLFDVHTDRTDFIFYFDRISHLLVEAALDTLHHYTPKTVACHNGNTFKGLVQSLELAAVSIVRSGDCFMNGVRKTFPAIPTGKLLIQSDSMTGEPQLHYENLPKSSEGSVYFLLDAQIISGAGAVMAIQVLIDHKVEQRNIVLISYLLTEIAVKRIFNVFPEVTIVVGKLSNTDTPDRSYNPEGFKDADWPFRTRFVDLLYFGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8G8</accession>
    <name>A0A1A0H8G8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710931.1">
        <property type="nucleotide sequence ID" value="XM_018857753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8G8"/>
    <dbReference type="GeneID" id="30030729"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291735" evidence="1">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="67" mass="7314" checksum="3B8F027609821781" modified="2016-10-05" version="1" fragment="single">KKIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPKVCYEGCLSLTPLYSSEPFYTVGEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Q2</accession>
    <name>A0A1A0H8Q2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">LNS2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_32396</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710913.1">
        <property type="nucleotide sequence ID" value="XM_018856042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Q2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8Q2"/>
    <dbReference type="GeneID" id="30029018"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000063">
        <property type="entry name" value="Nuclear elongation and deformation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057124">
        <property type="entry name" value="Ned1-like_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24565">
        <property type="entry name" value="Ned1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="4">
        <location>
            <begin position="446"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="105"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="343"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="667"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="713"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="134"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="670"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="730"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="818"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="884"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="925"/>
            <end position="948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="985" mass="107333" checksum="8C07D44F7A4592BB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H940</accession>
    <name>A0A1A0H940_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">Signal recognition particle subunit SRP68</fullName>
            <shortName evidence="10">SRP68</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_43044</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the signal recognition particle (SRP) complex, a ribonucleoprotein complex that mediates the cotranslational targeting of secretory and membrane proteins to the endoplasmic reticulum (ER). The SRP complex interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the SRP68 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710919.1">
        <property type="nucleotide sequence ID" value="XM_018857611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H940"/>
    <dbReference type="STRING" id="869754.A0A1A0H940"/>
    <dbReference type="GeneID" id="30030587"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15481">
        <property type="entry name" value="SRP68-RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3450.40">
        <property type="entry name" value="Signal recognition particle, SRP68 subunit, RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026258">
        <property type="entry name" value="SRP68"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034652">
        <property type="entry name" value="SRP68-RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038253">
        <property type="entry name" value="SRP68_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 68 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12860:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16969">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038995">
        <property type="entry name" value="SRP68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR038995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="585" mass="64940" checksum="BDA238253052D16D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9F9</accession>
    <name>A0A1A0H9F9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_230268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711178.1">
        <property type="nucleotide sequence ID" value="XM_018855245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9F9"/>
    <dbReference type="GeneID" id="30028221"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="35163" checksum="288B37846A679F31" modified="2016-10-05" version="1">MGIDSDEPPSGNVIDPMRVEDIFSIEDLSSISVSSVSANSDLLKDASDSIHIASINSAFDYSAFTNSVFEYSISAYVASTYVASAESASTYESSANSASNHIVSTYIISTHSASTYIACTESDSTSSAFNHNAFNDSAFDCIAFDYGTFDDGAFKYSALGDGDFGYGAFEYSAFEYSAFRNSDSTHSVSRSSVFNNSAFNNSAFTDGASSNTAFNYVLLIIWLPIIASNQSASTYISTTNSAFNHIASTKSASFHSAYTFIDSAYITSTHSASTHILCTKSAFVFSASNNSASTHGASACIASMDIASMDIASTYIASSHGASISMFFSYCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9G3</accession>
    <name>A0A1A0H9G3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rhomboid-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_27989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711049.1">
        <property type="nucleotide sequence ID" value="XM_018855346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9G3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9G3"/>
    <dbReference type="GeneID" id="30028322"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010821">
        <property type="term" value="P:regulation of mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000012">
        <property type="entry name" value="Rhomboid family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050925">
        <property type="entry name" value="Rhomboid_protease_S54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731:SF14">
        <property type="entry name" value="PRESENILIN-ASSOCIATED RHOMBOID-LIKE PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43731">
        <property type="entry name" value="RHOMBOID PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="8">
        <location>
            <begin position="102"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="256" mass="28171" checksum="2D28897FB2A2455F" modified="2016-10-05" version="1" fragment="single">RAVRPSSTWARYNRHYGRNKINWGSLGKPALFTAIFCIFTTVSTPYVMQLPVLSYFKSHPKAIVHAIIGLNVAGFLAWRSPLTSRYMSRFGLLMKDNIRTQWALLGSAFSHQDPFHLLFNMLMLYSFGTSFAMSIGSSNFLTMYLNSAVISSFISIAIPTIMRTSLSVASLGASGALFGVFGAFSYLAPKAPVALFFLPVPGGAWFLFLGSLAINAVGIAFKWGRYDYAAHLGGGLAGAAYGWYLSKLRESRIRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9U0</accession>
    <name>A0A1A0H9U0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Leucine carboxyl methyltransferase 1</fullName>
            <ecNumber evidence="3 8">2.1.1.233</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32731</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[phosphatase 2A protein]-C-terminal L-leucine + S-adenosyl-L-methionine = [phosphatase 2A protein]-C-terminal L-leucine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:48544"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="2.1.1.233"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the methyltransferase superfamily. LCMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.233" evidence="3 8"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711303.1">
        <property type="nucleotide sequence ID" value="XM_018856120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9U0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9U0"/>
    <dbReference type="GeneID" id="30029096"/>
    <dbReference type="OrthoDB" id="203237at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018423">
        <property type="term" value="F:protein C-terminal leucine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016651">
        <property type="entry name" value="LCMT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007213">
        <property type="entry name" value="Ppm1/Ppm2/Tcmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13600:SF21">
        <property type="entry name" value="LEUCINE CARBOXYL METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04072">
        <property type="entry name" value="LCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016305">
        <property type="entry name" value="LCM_mtfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 8 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR016305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016305-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="357" mass="38617" checksum="8C6200D8461C6177" modified="2016-10-05" version="1">MATRAAKDTVVRATDLDALSCRHSANTKGYFLPADPHVALLVGAYESALQHCDGYTQLAAGRTLRSSFGAPKLPLINRGTYLRTAAIDLVVSLFIAEHGACQVVSLGAGSDTRGFRVLQQHPGVSYTEIDFAESTRIKKLAISRLPALRRLVGAEDLAAIDVNSRADLAAMDPHLHGANYHLVALDLRDLAARGTDALAFLDSALPTLVVCECVLCYLPPKATVDVLAFWKQSLRHVAVVVYDPMGLGDAFGAMMAQNLGRRGLDMLSFSMYPDLPARKKLFEETLGFQAHLTDSALIGGYSGSPGPWLGAQEAARVGRLEMIDEVEEIALLLRHYCLIYAEAGLLLRFAAQLPWLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9V5</accession>
    <name>A0A1A0H9V5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12735</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711318.1">
        <property type="nucleotide sequence ID" value="XM_018854262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9V5"/>
    <dbReference type="GeneID" id="30027238"/>
    <dbReference type="OrthoDB" id="4018690at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="342"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="175"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="398"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="532" mass="59639" checksum="463E69566684925F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA46</accession>
    <name>A0A1A0HA46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein YTM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">YTM1</name>
        <name evidence="9" type="ORF">METBIDRAFT_40874</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NOP7 complex, which is required for maturation of the 25S and 5.8S ribosomal RNAs and formation of the 60S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the NOP7 complex, composed of ERB1, NOP7 and YTM1. Within the NOP7 complex ERB1 appears to interact directly with NOP7 and YTM1. The NOP7 complex also associates with the 66S pre-ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR12/YTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711385.1">
        <property type="nucleotide sequence ID" value="XM_018857323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA46"/>
    <dbReference type="STRING" id="869754.A0A1A0HA46"/>
    <dbReference type="GeneID" id="30030299"/>
    <dbReference type="OrthoDB" id="10251381at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000706">
        <property type="entry name" value="Ribosome biogenesis protein YTM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03029">
        <property type="entry name" value="WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012972">
        <property type="entry name" value="NLE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028599">
        <property type="entry name" value="WDR12/Ytm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855:SF11">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN WDR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19855">
        <property type="entry name" value="WD40 REPEAT PROTEIN 12, 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08154">
        <property type="entry name" value="NLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="NLE" evidence="8">
        <location>
            <begin position="6"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="152"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="295"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="384"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="466" mass="50337" checksum="B9DCF9BCE72BDB81" modified="2016-10-05" version="1">MSEAQVKARFFTKEDDETLRASDAPLFVPTSLKRYGLSEVVNHLLEKEEPVPFDFLIDGVLLRSSLDEYLTKNGLSAEATLQIEYTRAVLPPSFLASYNDDDWVSSIATINHASPAVAAQRGASLASPQILAGSYDGVVRTYNMSGLVETQYVGHLAPVRAVQWVYANRIVSAGNDRQVRLWKTAAAEGVGLVDDDDDKDDKDDKDAQSGRTLVILDAHKAPVVALDVSVASHRLLSAGSDGVVGFWSTVPKEMNVVEPYAVDASGAGASSALKKRRRMALKDSGVKHRAPLALLESHTQPVEGVVFDSRDSLVGYSVSQDHTIKTWDLVTSRCVDTRSTGYALLSVLQLPGVGLIASGSSARHINLHDPRTQAGATEKGVTKLLGHTNFVVDLAACPTNDTMFASASHDGTVKVWDVRADRAMYTITRESRTGKVFGVSWDREIGIVSGGEDKKVQINSNPSTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAD9</accession>
    <name>A0A1A0HAD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711352.1">
        <property type="nucleotide sequence ID" value="XM_018853994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAD9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAD9"/>
    <dbReference type="GeneID" id="30026970"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000016">
        <property type="entry name" value="mRNA cleavage factor complex component Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054127">
        <property type="entry name" value="Pcf11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021605">
        <property type="entry name" value="Pcf11_Clp1-ID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21936">
        <property type="entry name" value="Pcf11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11526">
        <property type="entry name" value="Pfc11_Clp1_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="1">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="510" mass="55637" checksum="C7F42DA81D9B98D5" modified="2016-10-05" version="1">MTRSPIVEDYASSLAELTFNSRPIIDNLTIIAKENTDSAAGILDAIADRIHKCIPEQKLFALYLLDSIVKTVGPPYNVLAGEQIFKLFSHVYLLVGEPTRARLVKIYELWRVTKTKGLGAPLFPPAQLARIGDFLRQAGYKKLDAVLPPEPPAGPTAASLVADIDAILPLLAHKLAANPADAALAGKKAALAELRVLLLSQALLPGELAGIQARLAPMKVPGAAPGPAAPPVAAPVPRAAALFSELVASGLVAVDQALVRGAVPEYTLVLPKHKHTPGKHGRRAASALERLLADANLTHKSQYEQIKYKELVKVLQWIAGAGAARDLQRFVTQAELEAATVQVLYETKALKCAQCGRRFTADAAGAARKRTHLDWHFRINQKQANFKSNVQLRSWYLDDYAWVQFRDDDLLEYEAAPRPERREPVPEAPAAEAYVVIPSTESNTSNMCTVCREQVKPTYKDALGEWVWDACMAVPGAAGKIVHVSCFEEASRKRGAPDDGDRLLKRERLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAI3</accession>
    <name>A0A1A0HAI3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78118</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711531.1">
        <property type="nucleotide sequence ID" value="XM_018858823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAI3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAI3"/>
    <dbReference type="GeneID" id="30031799"/>
    <dbReference type="OrthoDB" id="5592486at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF676" evidence="2">
        <location>
            <begin position="124"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="323" mass="36091" checksum="ED19929AEDFCCBAD" modified="2016-10-05" version="1">MPLPLHTVLITDKFRSLRDDYRPPKYPIVLCHGLSGFDTMTLLQFPEIRAFPGSKKPEHDLEKVAEEVNPSSSIMVNYWHGIEDALTKAGAKVITAKVPPFGTIADRAALLDALLREKLRDLPTDSADGRVKVNMVGHSMGGLDSRYLISKLQGPESPYRVVSLTTISTPHRGSEVADFVMSYVEKDQILQAMCPKAIPQLTTSFMAGFNETVRDDPSVAYFSYGAQMRPDGLRLFRATYEILKWEITRKGGLRVDNDGMVSVALAKWGKYVGTLDDVDHLDLINWTNRMKATVDQAMFQQPPAFNPIALYLDIADMLSRHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAZ5</accession>
    <name>A0A1A0HAZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rh-like protein/ammonium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_31754</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711675.1">
        <property type="nucleotide sequence ID" value="XM_018855908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAZ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAZ5"/>
    <dbReference type="GeneID" id="30028884"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015200">
        <property type="term" value="F:methylammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140157">
        <property type="term" value="P:ammonium import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF3">
        <property type="entry name" value="AMMONIUM TRANSPORTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="385"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="9">
        <location>
            <begin position="27"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="487" mass="53233" checksum="9E5E243DFCF2DA77" modified="2016-10-05" version="1">MSSNALLSARSTLEEQTLSFVAADMVYILFCSFGVFLITPAIGLFYGGTIRRKNIVQILFQSYMTTSVIVILWYLIGYSLAVSPTSTSKLLGDFNNAALYNDEAKPVSEGGNLPSVVNFCFNVFFPVATMQIFVGAIGERGRFLPSIVVGIIWTIVCYCPFAYWVWAPQGWLYQLGALDFAGGGPVHIASGIASLCYSWFLGPRGQPGKRTGSIFEPRGHSSVTTFIGVTLIWAAWLCFNSGTLLTVNVRTGYIFLNTILASCFACVAYAGTDKILTGKYSLKAACEGTIVGLVNITPSCGYYWPWAAALTSIINGVLCRLLVGFNHWVGIDDYSVSGVVHGFGGIIADTLTGIFASKTVAGYDGVTEIDGGWIDGNFKQIGLQIASWTSVVAWTFVFTMIIMFIVDKIPGLHLRATSEAEEMGMDLFEMAETLDEFGNDYEDFFRENATKWKAILHHYEDSVEILEGQSVSQGSVNHVHLVNREKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB06</accession>
    <name>A0A1A0HB06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_31852</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711819.1">
        <property type="nucleotide sequence ID" value="XM_018855932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB06"/>
    <dbReference type="SMR" id="A0A1A0HB06"/>
    <dbReference type="STRING" id="869754.A0A1A0HB06"/>
    <dbReference type="GeneID" id="30028908"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 13 16" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291895" description="candidapepsin" evidence="14">
        <location>
            <begin position="18"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="74"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="369" mass="40394" checksum="CA13BBD6CE1EFAA4" modified="2016-10-05" version="1">MLQLFTLLSLAAVLVQGLVVPELQARDSPAPLALDFSIQSSVGNLTVSEWRDKFAPKMSKRAYSEKISNVDYAYFLNVYLGSNRQKSNVLLDTGSSDLWVPTSDYNYKTSSTAKDTGHSFSIEYVDGSSAKGEYYKDTLTFDSGLPDVSLFEFAAASTDSFGVFGIADKDQESTRYTYDNFPWALQKAGITPKASYSLYLGEDHGTGVVIFGGIDTEKYEGSLTKYSIDTSDYALSLDVKTVKVGGKQVTENAPYVLDSGTSLALVSKELMSTLNDVFNVGSNGLVSCNQPSDEYLTFDFGENSIKVPYTDAVYSNGDGTCLLAFSYYEGYFILGDIFLRNAYVYYDLTDQTISLSQVKYSLASNIVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB37</accession>
    <name>A0A1A0HB37_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711613.1">
        <property type="nucleotide sequence ID" value="XM_018854057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB37"/>
    <dbReference type="GeneID" id="30027033"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291730" description="Secreted protein" evidence="1">
        <location>
            <begin position="32"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13658" checksum="FAD757A65C683B42" modified="2016-10-05" version="1">MSGCLGWLCARRLLSEVFVVCITAHKGPVHSGPLFCLAGRTLGYEACLGFWPKDSGDWYALLWNKRVTCWATCDRPGRVIVHCTSKFFELNWVVVLNPDDARTGTCDVIADGQEHGVMIVIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB80</accession>
    <name>A0A1A0HB80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Not1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_229635</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711658.1">
        <property type="nucleotide sequence ID" value="XM_018855230.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HB80"/>
    <dbReference type="GeneID" id="30028206"/>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007196">
        <property type="entry name" value="CCR4-Not_Not1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032195">
        <property type="entry name" value="CNOT1_HEAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16419">
        <property type="entry name" value="CNOT1_HEAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04054">
        <property type="entry name" value="Not1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat 1" evidence="12">
        <location>
            <begin position="254"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="11">
        <location>
            <begin position="507"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="10">
        <location>
            <begin position="721"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="9">
        <location>
            <begin position="921"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="8">
        <location>
            <begin position="1237"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="13">
        <location>
            <begin position="1548"/>
            <end position="1700"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-Not complex component Not1 C-terminal" evidence="7">
        <location>
            <begin position="1892"/>
            <end position="2255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1457"/>
            <end position="1486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1457"/>
            <end position="1470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1471"/>
            <end position="1486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2260" mass="255019" checksum="2F470A6A4FD01474" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBC0</accession>
    <name>A0A1A0HBC0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_69441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711693.1">
        <property type="nucleotide sequence ID" value="XM_018858379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBC0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBC0"/>
    <dbReference type="GeneID" id="30031355"/>
    <dbReference type="OrthoDB" id="4095286at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="41129" checksum="C6E2D2F74DFEF002" modified="2016-10-05" version="1">MSYFNGSSSGNNPLAFPSYNQQNLQQRNITGLSPANNPVNQLQNQYSESSLSAPLQQGENPASRQADEYINSESEVRNAQGSSASGTRSQQSLKKNRAEDGPKKTSLKKDRVNRPGQKFGAKKRLWVWSWFVQDLNDQNVAVCDFCGKVITRVTSDRGLPKKLVEHLHTHKLTKDLINASRPIPMDGSGNSYLSNLQTSYAGPFPQQSGNSGINDVVSSGTPGQIGLASPSQHPQQTYSSETQQLHPQSQVHISQLHTPNSNSQTQHSRRYILSKFENAPYSGPIFHRHLLKFLAENKLPIRILKLHSFQQLIYDLRPDSITDLLDLTGLYTSFVEVARAENLNETLSNNDSSIAETNVVNTLAQEMIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBD6</accession>
    <name>A0A1A0HBD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Cytochrome c oxidase assembly protein COX20, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31770</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the assembly of the cytochrome c oxidase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the COX20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711697.1">
        <property type="nucleotide sequence ID" value="XM_018855912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBD6"/>
    <dbReference type="GeneID" id="30028888"/>
    <dbReference type="OrthoDB" id="14603at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043069">
        <property type="term" value="P:negative regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022533">
        <property type="entry name" value="Cox20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31586">
        <property type="entry name" value="CYTOCHROME C OXIDASE PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12597">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007871">
        <property type="entry name" value="Cox20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="167" mass="18917" checksum="D8A2D5C5C7552887" modified="2016-10-05" version="1">MSWFGGSPSKVSAVKQVERSDTNDNSQYLDDLPPKFEDREPIHAKQPTYGDAFRNLKLSDFSLETYVGMPCFREAMITGFQSMGVLGFVTFLIHKNPGRSVNWAICGFFLGSVVGWEQCRSIRKKSFQTVEAARVANQDKVRRKLEDRSADDETLSQFNKTQTGECE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBL9</accession>
    <name>A0A1A0HBL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711782.1">
        <property type="nucleotide sequence ID" value="XM_018857380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBL9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBL9"/>
    <dbReference type="GeneID" id="30030356"/>
    <dbReference type="OrthoDB" id="2011986at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018810">
        <property type="entry name" value="UPF0662"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28086:SF1">
        <property type="entry name" value="CU(2+) SUPPRESSING AND BLEOMYCIN SENSITIVE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28086">
        <property type="entry name" value="UPF0662 PROTEIN YPL260W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10303">
        <property type="entry name" value="DUF2408"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="476"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="543" mass="61617" checksum="AB7355C7A66C3BA8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBQ1</accession>
    <name>A0A1A0HBQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hamartin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_41672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712074.1">
        <property type="nucleotide sequence ID" value="XM_018857439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBQ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBQ1"/>
    <dbReference type="GeneID" id="30030415"/>
    <dbReference type="OrthoDB" id="6022054at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033596">
        <property type="term" value="C:TSC1-TSC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032007">
        <property type="term" value="P:negative regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007483">
        <property type="entry name" value="Hamartin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15154:SF2">
        <property type="entry name" value="HAMARTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="943"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="754"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1031" mass="114895" checksum="9719E69DA64B7394" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS3</accession>
    <name>A0A1A0HBS3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_42771</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711971.1">
        <property type="nucleotide sequence ID" value="XM_018857584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS3"/>
    <dbReference type="GeneID" id="30030560"/>
    <dbReference type="OrthoDB" id="43906at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048250">
        <property type="term" value="P:iron import into the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758:SF4">
        <property type="entry name" value="MITOFERRIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45758">
        <property type="entry name" value="MITOFERRIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="24"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="132"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="225"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="320" mass="34314" checksum="EF8CE101C2050F75" modified="2016-10-05" version="1">MADHPHQLGADHLEVDYEALPQDASILAHLSAGAFAGIMEHTVMYPVDLIKTRMQMLAAGQPASRLVIAAISRVASGEGAYALWRGVSSVVLGAGPAHAVYFSAFEALKTFLVARFLAPGRSATSKWVTDESHPLIAAGAGIAATTASDALMTPFDVLKQRMQVLEAPAAGTRLWRTAAAVYRLEGVRAFYLLYPTTLFTNIPFAALNFGFYEYSSALLNPAHTYNPYYHCVAGGIAGGIAAALTNPLDCIKTALQTRGVSTNAHMRTVDGFASAARVMYQQGGAAAFTRGWRPRIIFNVPSTAISWTAYEMAKELLLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBY4</accession>
    <name>A0A1A0HBY4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PH domain-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_31917</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711897.1">
        <property type="nucleotide sequence ID" value="XM_018855945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBY4"/>
    <dbReference type="STRING" id="869754.A0A1A0HBY4"/>
    <dbReference type="GeneID" id="30028921"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF142">
        <property type="entry name" value="RAN-BINDING PROTEIN 3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="4">
        <location>
            <begin position="190"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="326"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="355"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="371" mass="39364" checksum="335A15990FC4AF3D" modified="2016-10-05" version="1">MTDGSPAKKRSISQQDAAAKKAKTDLPLDAEIAVNALASDSTSTGHRASSLADDGAPQKTTSTAEKEQNNPAPLSADADKPVFGTTALPGSAKPVFGAASTFGNASIFDRMKNNTNVFDAAAKPSEAAQEQAAPATLSFGSFGGSFGAGSKFSNALQKASQKKSFLDEPKETDTDKEVSLGPSSQQYKQVDLSEKKVETGEENEKSVYSATAKLFELDLSNISEGWKERGRGPVHVNQAIQDPAQARLVMRSNGLLRVILNYKISQTTTLLKGLEASLSPGKFLRMTAVSAEGKPVQYMLKFLSEEIRDALVDKVSELKEQIKLAASPKPETAAQPPATGFRFISDSSENEAGGENTTLQRLSTNVDPGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC57</accession>
    <name>A0A1A0HC57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial import inner membrane translocase subunit TIM23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_40947</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712229.1">
        <property type="nucleotide sequence ID" value="XM_018857334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC57"/>
    <dbReference type="STRING" id="869754.A0A1A0HC57"/>
    <dbReference type="GeneID" id="30030310"/>
    <dbReference type="OrthoDB" id="159299at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005681">
        <property type="entry name" value="Tim23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045238">
        <property type="entry name" value="Tim23-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00983">
        <property type="entry name" value="3a0801s02tim23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371:SF0">
        <property type="entry name" value="SD19278P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15371">
        <property type="entry name" value="TIM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="208" mass="21923" checksum="AFC367877A501A9B" modified="2016-10-05" version="1">MSWLFGGKSKDQTQNNVDQSLGFDPALVSDVSTIILAPGVLDSSKLHPLAGLDKGIEYLDLEDDQLTDLENGSSIIAARNWRDNLCYGTGSLYLLGLGVGGAYGMQEGLSNLPANSTAKLKLNTVLNNITKRGPFMGNSCGVLALIYNIIDSTIDNFRGKHDDVNSLAAGALTGALFRSSAGVKPMAYSTAIMTAGAGAWCGLKRFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC68</accession>
    <name>A0A1A0HC68_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_186579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711993.1">
        <property type="nucleotide sequence ID" value="XM_018854804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC68"/>
    <dbReference type="GeneID" id="30027780"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="14430" checksum="2AFDADF65A5CD861" modified="2016-10-05" version="1">MSYIRIGAAEKRFGRQTHHQKSRGGTWLSQPVQARDVPRVEFCTWHACGAVCRRNTLRTRVVGCSEEASRAKMSFFIFGMVPALLWCCMAWHVRMCHVLLGWLCFGWEEHISPILPFFVLVDFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCA5</accession>
    <name>A0A1A0HCA5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Macro domain-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712017.1">
        <property type="nucleotide sequence ID" value="XM_018854145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCA5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCA5"/>
    <dbReference type="GeneID" id="30027121"/>
    <dbReference type="OrthoDB" id="6082470at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028071">
        <property type="entry name" value="Macro-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14519">
        <property type="entry name" value="Macro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="20"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="268" mass="28497" checksum="421A7B2FA5064C6D" modified="2016-10-05" version="1">MKFVVVNSCPSTIRCFRSAFVEVSKALPRHIGLECFQGSLADYVHSLHCPENQNTAVVSPANSLCFMGGGFDRGIAELLGERGSGYRAVEAAIQNHVLSRHHGFVPPQASSVVDLQEALGGGHASGSKITHLVLSPTMAVPERISYTTVFYCMWNTLLAMDGPFDTVVLPAIGAGYGGVPAAVAAATISGALGLFYMRMGSPLNRGAAVLLFLRKDYRALGIPADIARIESVVAHKEEAISGAKNHGPAEHEGPLEWRELSRILDSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCD1</accession>
    <name>A0A1A0HCD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_42213</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712073.1">
        <property type="nucleotide sequence ID" value="XM_018857513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCD1"/>
    <dbReference type="STRING" id="869754.A0A1A0HCD1"/>
    <dbReference type="GeneID" id="30030489"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097586">
        <property type="term" value="C:dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900101">
        <property type="term" value="P:regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23285">
        <property type="entry name" value="beta-trefoil_MIR_PMT4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF51">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="593"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="639"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="708"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="323"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="395"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="755" mass="86298" checksum="4B4B51D03CABCA32" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI5</accession>
    <name>A0A1A0HCI5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Octanoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_78535</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>octanoyl-[ACP] + L-lysyl-[protein] = N(6)-octanoyl-L-lysyl-[protein] + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the LipB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712138.1">
        <property type="nucleotide sequence ID" value="XM_018858895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCI5"/>
    <dbReference type="GeneID" id="30031871"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016262">
        <property type="entry name" value="LPLase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="9">
        <location>
            <begin position="90"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="136"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="8">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR016262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR016262-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="296" mass="33496" checksum="27A2B72816AD9D34" modified="2016-10-05" version="1">MRMFLVRRFSSACASKFQPLNEDYRTLRHIHFDGVTPFVKGQAIQNKMVSANLDFKKMEATIKKKQKAFALQGLQLQEFEQEFIQRVLSMKPFPTLLTFEFDGVYTGGKQMKQDPTLPEQIAAFNRLGCEYHQLERGGQVTWHGKGQLTAYLIMDLKQFSDLTVRCYVDSVLLKAVQNLLEKRFGLPSFVNDNPGVWMSPDNLKICSVGCNIQRAITSYGIGLNVRPDLKFLNTHIMCGMPGTRATSIQELKPEYSGSIKEVGENFAQELAKRLNLSTIEHMNGADLLAEESLNAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCL6</accession>
    <name>A0A1A0HCL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P/MRP protein subunit POP5</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_39205</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712345.1">
        <property type="nucleotide sequence ID" value="XM_018857113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCL6"/>
    <dbReference type="GeneID" id="30030089"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000171">
        <property type="term" value="F:ribonuclease MRP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034965">
        <property type="term" value="P:intronic box C/D snoRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="176" mass="20070" checksum="8710ED585AB7A32D" modified="2016-10-05" version="1">MVRLKHRYILFEILYPPSAYGTSREQKEKFSEFSQTPKDALLHMHSPSPSAVSPRSILLLLKRVIVDHYGEFASGTVGSLIIVKYFSNRTSTGILRCSRNDFHIVVAALALIDKIETHRVVVRSVHVSGTIRKCEDFSIRRARKLMADLGKEDEVERGLDEFISMFKSGDREDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCM5</accession>
    <name>A0A1A0HCM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleic acid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_32209</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712256.1">
        <property type="nucleotide sequence ID" value="XM_018856008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCM5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCM5"/>
    <dbReference type="GeneID" id="30028984"/>
    <dbReference type="OrthoDB" id="274995at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001677">
        <property type="term" value="P:formation of translation initiation ternary complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="3">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="95" mass="10858" checksum="2C977F9B9661742D" modified="2016-10-05" version="1">MGHIRGKLRKKVWMGQGDIILVCLRDFQDDQCDVVHKFNSDEARTLKSMGELPESAKINETDTFGADDDEEVNFEFGDDDDDDDDDDDELDIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD08</accession>
    <name>A0A1A0HD08_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA 3'-end-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77495</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CPSF4/YTH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712465.1">
        <property type="nucleotide sequence ID" value="XM_018858708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD08"/>
    <dbReference type="STRING" id="869754.A0A1A0HD08"/>
    <dbReference type="GeneID" id="30031684"/>
    <dbReference type="OrthoDB" id="1914176at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000012">
        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045348">
        <property type="entry name" value="CPSF4/Yth1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="209" mass="24798" checksum="EA9900334B8B0B11" modified="2016-10-05" version="1">MLSLNPIIHPDTKNKRFKFEPFLRKEFNFGLDPDRPVCQFYKPNQPDSCPNGPFCPNKHVASMFNNKVVCKHWLRGLCKKNDHCEFLHEYNLRKMPECLFFSKNGYCTQMPECLYLHVDPQQKIPECLQYERGFCPEGPNCPNRHVRKILCPMFLTGFCPKGYECDYTHPKFDNLPPKLRVEHKRLIKGETREVEKISYDQPLTEVRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDF7</accession>
    <name>A0A1A0HDF7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77558</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712547.1">
        <property type="nucleotide sequence ID" value="XM_018858721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDF7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDF7"/>
    <dbReference type="GeneID" id="30031697"/>
    <dbReference type="OrthoDB" id="10262026at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007192">
        <property type="entry name" value="APC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04049">
        <property type="entry name" value="ANAPC8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cdc23" evidence="8">
        <location>
            <begin position="7"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="374"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="408"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="442"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="579" mass="65191" checksum="5DAB43BFF79A3C20" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDT6</accession>
    <name>A0A1A0HDT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">RuvB-like helicase</fullName>
            <ecNumber evidence="15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_67411</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RuvB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712584.1">
        <property type="nucleotide sequence ID" value="XM_018858364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDT6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDT6"/>
    <dbReference type="GeneID" id="30031340"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097255">
        <property type="term" value="C:R2TP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002221">
        <property type="entry name" value="RuvB-like 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000010">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000002">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0010">Activator</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="16">
        <location>
            <begin position="68"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="469" mass="50842" checksum="385FDF3BEA6C03F0" modified="2016-10-05" version="1">MTSTIATTSSQTRDLNGLSLIASHSHISGLGLDDNLVPKPSAQGMVGQLKARRAAGLILKMVQVGQIAGRAVLIAGPPSTGKTAIAMGLSQSLGEGVPFTAITGSEIFSLDLSKTESLTQAFRKSIGIKIKEETEIIEGEVVEIQIDRSITGGHKQGKLTIKTTDMETIYELGNKMIEGLTKEKVLAGDVISIDKASGKISKLGKSFARARDYDAMGPETKFVQCPEGELQKRKEVVHTVSLHEIDVINSRQQGFLALFSGDTGEIRSEVRDQINTKVAEWKEEGKAEIVPGVLFVDEVHMLDIECFSFINRALEDDFAPIVVMATNRGVSPARGTNYKSPHGVPMDLLDRSIIIHTANYSADELRTILSIRATEEEVDLTGDALALLTKIGEETSLRYASNLIAVSLQLAAKRRAESVDLADVKRAYLLFLDSSRSVQFVSDFASRFIDDDGNVTLGTKADDDKMQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW2</accession>
    <name>A0A1A0HDW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_27095</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712780.1">
        <property type="nucleotide sequence ID" value="XM_018855330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDW2"/>
    <dbReference type="GeneID" id="30028306"/>
    <dbReference type="OrthoDB" id="5894at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051964">
        <property type="entry name" value="Chaperone_stress_response"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44029:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="4"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="467"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="517"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="258"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="476"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="541"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="601" mass="69695" checksum="78FEAD3115407A04" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ7</accession>
    <name>A0A1A0HDZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPS1</name>
        <name evidence="8" type="ORF">METBIDRAFT_11009</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712618.1">
        <property type="nucleotide sequence ID" value="XM_018853900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDZ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDZ7"/>
    <dbReference type="GeneID" id="30026876"/>
    <dbReference type="OrthoDB" id="9834376at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03122">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_eS1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027500">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylalanine; partial" evidence="6">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="256" mass="29026" checksum="AFBCFA4B90769277" modified="2016-10-05" version="1">MAVGKNKRLSKGKKGMKKKVVDPFTKKEWYDIKAPTTFENRNAGKTLINKSTGLKNASDGLKGRVFEFCLADLQGSEDHSYRKVKLRVDEVQGKNLLTNFHGLDFTTDKLRSLVRKWQSLVEANVTVKTADDYVLRVFAIAFTKRQANQVKKTSYAKSSKLRDVRKKMIEIMQREVSNVTLAQLTSKLIPEVIGREIEKSTQSILPLQNIHIRKFKLLKQPKFDLGSLLALHGENTTEEKGKKVSSGFKDVVLETV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ9</accession>
    <name>A0A1A0HDZ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_77764</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. This subunit is a non-integral membrane component of the membrane pore domain and is required for proper assembly of the V0 sector. Might be involved in the regulated assembly of V1 subunits onto the membrane sector or alternatively may prevent the passage of protons through V0 pores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712820.1">
        <property type="nucleotide sequence ID" value="XM_018858759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDZ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDZ9"/>
    <dbReference type="GeneID" id="30031735"/>
    <dbReference type="OrthoDB" id="10250083at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1690.10:FF:000003">
        <property type="entry name" value="V-type proton ATPase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="350" mass="40448" checksum="8ED73BCD0E023AD6" modified="2016-10-05" version="1">MEGLFFNIDYGYVEGVVRGYRNGLLTLNQYVNLTQCDTLQDLKLQLSATDYGNFLASQAGPLLTSIIQDRLSKRMYLEFQYLRGQSTGKLARFMDFISYGYMIDNVLLMITGALHERDLADILPKCHPLGWFDTLPTLSTATDIDSLYDVVLVDTPLAPFFKDCLTLHDLDDLNIEIIRNRLYRNYLEAFIKFVEQEFDGPDLEIMTRLLSFEADKRVINIALNSLHNAELSAEDKLGLFPRFGQLYPVYHNELASAEEVEQIKAVVESIGEFRDIFSDSQGTGGQKNIEDWFYLLEMRYNKNAFTQQFTLSSVWAWLRSKEQEVRNITWIAECIAQNQKNRIDNYISVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE38</accession>
    <name>A0A1A0HE38_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_147876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712663.1">
        <property type="nucleotide sequence ID" value="XM_018854540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE38"/>
    <dbReference type="GeneID" id="30027516"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="126" mass="13468" checksum="23F497CF31B35565" modified="2016-10-05" version="1">MSFGRRGTMKPRSSFSKASVGWMLSRSMLSRSAGSAAPRSQGPQSDGSSSLYADTRGIVAQNKPRVNLSPKSKNHSYVRYRPATHAKDPGAGCHSAARPHALADEDWACTCARAVRKNALFGGLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE88</accession>
    <name>A0A1A0HE88_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rho GTPase activation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_14861</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712714.1">
        <property type="nucleotide sequence ID" value="XM_018854550.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE88"/>
    <dbReference type="STRING" id="869754.A0A1A0HE88"/>
    <dbReference type="GeneID" id="30027526"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176:SF129">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN AT 16F, ISOFORM E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23176">
        <property type="entry name" value="RHO/RAC/CDC GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="139"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="383"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="587" mass="67152" checksum="E73E0D8B0A2A4EEC" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB0</accession>
    <name>A0A1A0HEB0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_38851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712733.1">
        <property type="nucleotide sequence ID" value="XM_018857058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEB0"/>
    <dbReference type="GeneID" id="30030034"/>
    <dbReference type="OrthoDB" id="3998251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025928">
        <property type="entry name" value="Flocculin_t3_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13928">
        <property type="entry name" value="Flocculin_t3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="180" mass="18887" checksum="4C5F7D99D519B494" modified="2016-10-05" version="1">MDHDITSHVVSTVRSFDKHSAATTISDVHTTVVTTTYCNENECSVSPVTTGVSYFTTIVHGTKTTYTTYCPLSTMIETVSSEIASSIDTTNGPNSVSIVSATDNIAATSSIKTFTVNAPAESTVNVITNNILTQTKTVSAVSTPGSSIKTQSTAIISSFEGAAARAFPAAIVLPFIYFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEE9</accession>
    <name>A0A1A0HEE9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_150121</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712773.1">
        <property type="nucleotide sequence ID" value="XM_018854560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEE9"/>
    <dbReference type="GeneID" id="30027536"/>
    <dbReference type="OrthoDB" id="4077205at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="37251" checksum="D7BB4544B8422FE0" modified="2016-10-05" version="1">MLHEQNAPIFSLLPSLAPGLIFQTTYSNVIVKFIRDEMARLGQSQPTVDRLEHVKDQLLKSTLHSGEYTLEVDEQKSLILAYAFGEIYTPALALVTGKHITVQFWGAGEEQPGQPMIRNCVLNPESCDPIFFEVLDDPQIDKRRGVLDVNPFLQVRGMVSKLGLLLNLSTFDLEELSDLMAAPENSGGQKALFPANLGVPTLETLYERSDNGLKYLVRAKEPEETKTNGRPNFSYSSKSIVDPNNWFCSCSDFTSASFVIHDASGSDLLGDTGHELLSRLFRAAVCKFPKSLPICRHLLAVLLMLFNWDTAWNAGLVRVCWIRSETEQTELRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEI8</accession>
    <name>A0A1A0HEI8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_152968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712896.1">
        <property type="nucleotide sequence ID" value="XM_018854581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEI8"/>
    <dbReference type="GeneID" id="30027557"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="301" mass="31973" checksum="FCB658D96E332B3C" modified="2016-10-05" version="1">MGVFVWRPVGKTGGSEKNSCERRLLDLGATRSTPDSGAPVQRHVSYTHIVLSLYKKPGGRAARPRKKNCFPPGASAGTTLGASAGLPRAGGPGAASPRFNPQSRCAGASTGPGWPLIWRQASTASGTPSGRRRGARRVFPRSSSVMLLAPLRWGRRTFGHPKTDPDAANRSAALPSRRVSWAQGAGHGRGQGSATCLCGWAQRAMPVFWPCSGASTWICVFDATRAGHTTVEYASTGAGCPGVHPQRVFDCACGRGREVCLWPLRVLARLQPGPKDRRCLCRAESGHISPGHPWAAIEHMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEN0</accession>
    <name>A0A1A0HEN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="9">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_77777</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712843.1">
        <property type="nucleotide sequence ID" value="XM_018858761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEN0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEN0"/>
    <dbReference type="GeneID" id="30031737"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.465.10:FF:000014">
        <property type="entry name" value="D-lactate dehydrogenase (Cytochrome), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF111">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="13">
        <location>
            <begin position="119"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="578" mass="62606" checksum="9D9567991CD3F258" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEV7</accession>
    <name>A0A1A0HEV7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_153289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712918.1">
        <property type="nucleotide sequence ID" value="XM_018854585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEV7"/>
    <dbReference type="GeneID" id="30027561"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="161" mass="18505" checksum="29E1A087C54C15F6" modified="2016-10-05" version="1">MSRSPLSLDLSFQLFAPSLHSLRFYPLAVSAPHLFTFCLKNASHTQSILWSISTTMEHKQSQGAYAFSQNCMDIQTVWLNTNRHPRIPSLIKAPSFITSSAYKPHCSSMPWCSVHGPRSTRTHGRLHTMAHPPWGPSFRYRCRKRFFLSFIFFTGIIALRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFC9</accession>
    <name>A0A1A0HFC9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9078</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713187.1">
        <property type="nucleotide sequence ID" value="XM_018859312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFC9"/>
    <dbReference type="GeneID" id="30032287"/>
    <dbReference type="OrthoDB" id="4022827at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="39420" checksum="97AF306A9D812F28" modified="2016-10-05" version="1">MPAAVFAAQARPAPGAAVVLFGRPVLQRLAEKAPPFVLGMLATYVLQLAVPTVAYYTAATARLLKAAFLWLALLVAVAWLAGLGPVQAHDLAGMARRVAANFQHGPVAPGPQNRPGRSYRRASALVPEPRSPERGAGPGRKTPCSPSRQRPKAGPCAPGPVHVPSFVVPVRDSLHKACSDAVFPHQPVILRPVILRPGARRHGSDSEGVRDEAHPKDNRATGQLLLPAARDTGLALPATGMGGVSGRRLAYPQEPPRPSSHVFLRDNGGVSFETRPDSAGSLETRRGSAGSFETRLDSPGSPRTRRNSAGSPKTRLDSAGTAAVSSVPGLPADGALVHGQKSGPGNAGFLGRTMSVLSEQSVLGTRANYSKFLANVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFG0</accession>
    <name>A0A1A0HFG0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbon-nitrogen hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713217.1">
        <property type="nucleotide sequence ID" value="XM_018856743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFG0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFG0"/>
    <dbReference type="GeneID" id="30029719"/>
    <dbReference type="OrthoDB" id="201515at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008418">
        <property type="term" value="F:protein-N-terminal asparagine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070773">
        <property type="term" value="F:protein-N-terminal glutamine amidohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039703">
        <property type="entry name" value="Nta1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11750:SF26">
        <property type="entry name" value="PROTEIN N-TERMINAL AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="328" mass="36325" checksum="4E60B7A534E29564" modified="2016-10-05" version="1">MRLRIACLQLNPLLGKVEANVAKINRMMADCQKEIDLLVLPELAVTGYNFKLAKEIEPYLEKSGEGASFKIARLLSQKFKCTTVIGYPEKFGSFTYNSALVVDEKGYVVSNYRKSHLYETDEAWGCSENPVRGFPPFTLLLPDKTGAKGEKVGLVTANIGICMDMNPYQFKADFNEFEFSMNCWKNDASLIIVPTAWLSSDSPSIQEGLSEIEKANKAALYQDQIAANSLGNAGVPTQSLVDYWIVRLFPFLAHPYNGLPRKTKRTTVVICNRVGIEDQVLYGGSSSIMQFDPLKGESDALDASNPSVDVVASAGWASEEMIYHEVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFI4</accession>
    <name>A0A1A0HFI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_29478</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713390.1">
        <property type="nucleotide sequence ID" value="XM_018855411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFI4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFI4"/>
    <dbReference type="GeneID" id="30028387"/>
    <dbReference type="OrthoDB" id="440325at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018484">
        <property type="term" value="F:4-hydroxybenzaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047770">
        <property type="term" value="F:carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046185">
        <property type="term" value="P:aldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07135">
        <property type="entry name" value="ALDH_F14-YMR110C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000025">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000004">
        <property type="entry name" value="Aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570:SF16">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE TYPE III, ISOFORM Q"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="8">
        <location>
            <begin position="47"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="61315" checksum="FF10A5EF3FD1EE36" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFU6</accession>
    <name>A0A1A0HFU6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA damage-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_29325</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713209.1">
        <property type="nucleotide sequence ID" value="XM_018855381.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HFU6"/>
    <dbReference type="GeneID" id="30028357"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018846">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10433">
        <property type="entry name" value="Beta-prop_RSE1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 first beta-propeller" evidence="5">
        <location>
            <begin position="95"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="6">
        <location>
            <begin position="528"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="4">
        <location>
            <begin position="925"/>
            <end position="1254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1288" mass="141327" checksum="571D46C6719C9E9C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFX2</accession>
    <name>A0A1A0HFX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713382.1">
        <property type="nucleotide sequence ID" value="XM_018859342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFX2"/>
    <dbReference type="GeneID" id="30032317"/>
    <dbReference type="OrthoDB" id="4095763at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021216">
        <property type="entry name" value="DUF2722"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10846">
        <property type="entry name" value="DUF2722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="419" mass="46147" checksum="2677B24334E59C08" modified="2016-10-05" version="1">MKVCSLIETIPDAEDPLPLMSKHTSEPPYRQPESRDTSARNDAICPSAETSCHDTSKGPPTGINNTYTSKYGHRSSHNSQVVPESVSTSLLGPGVKGFAMSPSMFEEALRLRAEQERTEQERLKLEVISKTKSLIQFAIENNVPSESLSIVSMHNQSNRANQSLPTQALGQVSNSNNSAGINDSSGALYLRSPYLTASPLQSSREYENSNNASSVDPMNFRFGGAQSRYQLSSHNINNKRPRSPAKIGAMAVASLANPLTPFRPATRTIPLHQRHYSMPTEITVSDHKSGNHLHAQLKNQNISDSTNRLKSPEGVKSSARVRPIPAQNIQAGKGPNVLQDDMTQHQQVIQFHHWISENPGKRPAEKSEHKRSNSMSLQSLAAIHKRHRSTDISMDLSRVGSMPNVQRDASFCTSSERTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFX7</accession>
    <name>A0A1A0HFX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein MGR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_38544</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MGR2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713387.1">
        <property type="nucleotide sequence ID" value="XM_018857018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFX7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFX7"/>
    <dbReference type="GeneID" id="30029994"/>
    <dbReference type="OrthoDB" id="5409308at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018450">
        <property type="entry name" value="Romo1/Mgr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28525:SF1">
        <property type="entry name" value="REACTIVE OXYGEN SPECIES MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10247">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01378">
        <property type="entry name" value="Romo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="10941" checksum="BEDF1C6AD0B8E708" modified="2016-10-05" version="1">MAVQYHQPSNFDKFKMGALMGGSVGVCVGVLFGGFTILQHGAGPNGVMRTLGNYIMGSVATFSLFMSIGSVIRSDTGYLAMERTLVQSQRQKIRELYGLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG63</accession>
    <name>A0A1A0HG63_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713329.1">
        <property type="nucleotide sequence ID" value="XM_018859332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG63"/>
    <dbReference type="GeneID" id="30032307"/>
    <dbReference type="OrthoDB" id="4077766at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="510" mass="55924" checksum="271F10479659E036" modified="2016-10-05" version="1">MKSSPDEDWSIISSSDYEEDLASSLSVSRQNGGKNSTENYELDMASASVSTVRPLSQSGNLAGSRFLDSLGCSQGGAPGLGLRGAPGANGASDRGPGGAEPILAPAAPWSLRLAQLLAPYSAARRAAACGLQSLRESNVNLKSRLRKMFHPSLARMRSRCERFSQPRLRSALLATVAVLEAQEHLLVYFAILAVVATCAGGVLGANLLVSRHLYAGPARTWGSFKQSKSGSCEAGNSCVRVCLWVQRAFDLLATIGGQCSEKTRGALDWVLYEDIQEKQGWFGHNMRARKLRFGRLAVQMNTHLNVAWNSLQGLRTAQAYAEFRDMFSAQNFSRARGFVRARASALAASLDMEARLAQWRCGLGLFATQTLHAYKTFSVKSWKHISSASQIGWDFGSRTLRTALESAKKRTQVENTEDLKKMFLRQSQVLRGSLVEKYERISAEALGLGASFLLMASKYQTRIDSVEKRARMFDSVKQASQGKVNLWTRRIQVAALEAFRLSRNMTELAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGE1</accession>
    <name>A0A1A0HGE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_102286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713715.1">
        <property type="nucleotide sequence ID" value="XM_018853717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGE1"/>
    <dbReference type="GeneID" id="30026693"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="27979" checksum="23996F78449EF51A" modified="2016-10-05" version="1">MELSWRQVRTKPAFFQRQQKETRAYRAARKRRLREMDPSARSHGHLYAFLTGRRNYHLRGHCRKECPAPPRPCVRRAALSHGNQTVRHSHRVSPAPESPIRLWTKTSASPPCPNTGKTASSICGCLLCGQGPALLAPVCLDITRQFARLGQRMQGAALCARRLRLSRQPVPHIPRWALGSTLQRQFEIAGAAWRQARGWPETTDMGAARLHKRAHGFFGHTACALPCVLFGPIYIYMCFIFIFFWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGR3</accession>
    <name>A0A1A0HGR3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial</fullName>
            <ecNumber evidence="5">1.1.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Isocitric dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">NAD(+)-specific ICDH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_63507</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NAD(+) = 2-oxoglutarate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:23632"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.41"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Octamer of two non-identical subunits IDH1 and IDH2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.41" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713667.1">
        <property type="nucleotide sequence ID" value="XM_018858332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGR3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGR3"/>
    <dbReference type="GeneID" id="30031308"/>
    <dbReference type="OrthoDB" id="10261637at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045242">
        <property type="term" value="C:isocitrate dehydrogenase complex (NAD+)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000001">
        <property type="entry name" value="Isocitrate dehydrogenase [NAD] subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004434">
        <property type="entry name" value="Isocitrate_DH_NAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00175">
        <property type="entry name" value="mito_nad_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF42">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="14">
        <location>
            <begin position="31"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="359" mass="38612" checksum="96045E1E86FBEF57" modified="2016-10-05" version="1">MFRSAAKNVRTLATAASPEAVLPKKYGGRYTVTLIPGDGIGKEITDSVQTIFKDQNVPIDWEVVDVSGLGEDSQGVTEAVNSLKRNKVGLKGILYTPTNTHGKSLNVALRKELDIYASLVLIKNIPGVKSRLDGIDFALVRENTEGEYSGLEHLSFPGVVESLKIMTRFKSERIAKFAFDFAKKNNRKLVTAIHKANIMKLGDGLFRTTVKDVAQDYPGIEVSDLIVDNASMQAVAKPEQFDVLVTPNLYGSILSNIGAALVGGPGLVPGANFGREHAVFEPGCRHVGLDIKGQNTANPTAMILSASMMLRHLGLNSHADNISKATYDVIAEGNVRTKDIGGTSSTTEFTNAILTKLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGV0</accession>
    <name>A0A1A0HGV0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713701.1">
        <property type="nucleotide sequence ID" value="XM_018855471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGV0"/>
    <dbReference type="GeneID" id="30028447"/>
    <dbReference type="OrthoDB" id="10287216at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="220" mass="25207" checksum="E7B264C1C51BF50E" modified="2016-10-05" version="1">MLLQRIQDKSTYEVPSNLRDFHEVMMLARKQACAATENVICAFESVSSIGKHFIGVINRLQASATRFLMLNESRQSFDSAGNNIYEQHLCECLTGCEDLQQDILLFKDVISQSEELMADNTRHISTSMRNTIVYSKKIKDTFAEIYKNDESSWQHIQAVISASEAYKKCTSFLTEMTPERLFRFLKHDFAKLQADVKNIDQICGLLLDQTRKTLTNSQSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGZ1</accession>
    <name>A0A1A0HGZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SCA7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713741.1">
        <property type="nucleotide sequence ID" value="XM_018859405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGZ1"/>
    <dbReference type="GeneID" id="30032380"/>
    <dbReference type="OrthoDB" id="4072710at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="1">
        <location>
            <begin position="65"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="27912" checksum="43626DAAE1A0B3DD" modified="2016-10-05" version="1">MFCDLTPEPISSPPVILSPLRKLHRPGLILENTSSALPPVHSYDGFDAVCNLTPSQILSIQEDQTDYICGVQTNAHVCRNSFYCRRHLLEQRMEVRRSLPLKCLMEEELKNVAWFRKRRVLTKLYHDCKSYWHKHPSASTTPMTCSRTSCVQNESNVSSSPLCASVSSYNAPGTAARIEESTSSLLKKAFLKRLSRSADLHKDDIVSYLFYKLACYEEAGIDMRARELEARYSASENDLDIDQNGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH59</accession>
    <name>A0A1A0HH59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cytochrome c oxidase subunit IV</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_38491</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase IV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713817.1">
        <property type="nucleotide sequence ID" value="XM_018857012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH59"/>
    <dbReference type="STRING" id="869754.A0A1A0HH59"/>
    <dbReference type="GeneID" id="30029988"/>
    <dbReference type="OrthoDB" id="186013at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.442.10:FF:000002">
        <property type="entry name" value="Cytochrome c oxidase subunit V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.442.10">
        <property type="entry name" value="Cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004203">
        <property type="entry name" value="Cyt_c_oxidase_su4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036639">
        <property type="entry name" value="Cyt_c_oxidase_su4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707:SF10">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10707">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02936">
        <property type="entry name" value="COX4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81406">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16819" checksum="84BA912AF7F5A3C0" modified="2016-10-05" version="1">MLRQSIVRAVRFNSTVAKAAPRPLSSAYVNHLETRWNKLPKEDQTALIDELKARMELPWQELTSGEKKAAYYISFGEWGPRKPLYAPGDKSKIFWGTVAGLLAGVGLFASLRMMAPADPITLNKEWQQKSDEYLKSKNANPFTGYSQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH99</accession>
    <name>A0A1A0HH99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein 70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_36897</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713856.1">
        <property type="nucleotide sequence ID" value="XM_018856796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH99"/>
    <dbReference type="STRING" id="869754.A0A1A0HH99"/>
    <dbReference type="GeneID" id="30029772"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000014">
        <property type="entry name" value="Chaperone protein DnaK HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000003">
        <property type="entry name" value="Heat shock protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="647" mass="71023" checksum="7BC67A72434366C0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHD6</accession>
    <name>A0A1A0HHD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Manganese/iron superoxide dismutase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_102810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713734.1">
        <property type="nucleotide sequence ID" value="XM_018853728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHD6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHD6"/>
    <dbReference type="GeneID" id="30026704"/>
    <dbReference type="OrthoDB" id="275227at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="2">
        <location>
            <begin position="202"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="261" mass="29726" checksum="2D9C58597FF72BD4" modified="2016-10-05" version="1">MFSRAIRHTASISKNQHVAAVRMLHEVPVLKNHETFAKNGIQGLYSAEGYATAWSDYQKLLTTSLTLNTVGTRHEGRSAFQLLLLTAKRTTDQHIFHYASQAHNNHMFFEQLADKEHAASTRPSRFLLERIAGGSSLDSLKTEIIRLAESAEGQGWVFLVEDPDKSFRLLKCNNDGTPYYHGKNQSLDLNANIDPQSFEELTQIKQKGDNAELDFTLPVLAINFWDVAFVTDYGITGKREYLERVLDHINWDVINKRMFQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHR6</accession>
    <name>A0A1A0HHR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37825</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714027.1">
        <property type="nucleotide sequence ID" value="XM_018856919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHR6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHR6"/>
    <dbReference type="GeneID" id="30029895"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000827">
        <property type="term" value="F:inositol-1,3,4,5,6-pentakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000825">
        <property type="term" value="F:inositol-1,3,4,5-tetrakisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000823">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 6-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000821">
        <property type="term" value="P:regulation of arginine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF103">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE MULTIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="357" mass="38624" checksum="8E50B01E2A0DFD61" modified="2016-10-05" version="1">MNFTPSVHQAAGHDGALTAGPVFAKLLSPQEISFYAQASSRLSAEPELLGSQLAHWMPTYMGTMNEGFERVEGAMVLDPDGVSSSVNPAVSAPDLPAVSAPDLPAGLENPYLVLLNLYHRFVHPCILDIKLGSVLTDDTVLLEKKARLEAVSKATTSGSLGFRICGMKIFSQTPLNPAPLFPDMDATMHTEAIEDGGLYTSFDKIFGRSLDKSSVGKGLGLFFLVEPQVHDLLLSRFVQRLQLLYNCLLDAEVRIKSGSLLFIYEGDPARWAGVNGDSYTEADPLVCDPFYAVDSGADSDADSDVESDFEHRKAPLSRLNLIDFAHAKYAPGKGPDDNILDGLENLLDVFCRLADLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHV8</accession>
    <name>A0A1A0HHV8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hap4 transcription factor heteromerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_9697</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713909.1">
        <property type="nucleotide sequence ID" value="XM_018859441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHV8"/>
    <dbReference type="GeneID" id="30032416"/>
    <dbReference type="OrthoDB" id="5374328at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018287">
        <property type="entry name" value="Hap4_TF_heteromerisation"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10297">
        <property type="entry name" value="Hap4_Hap_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hap4 transcription factor heteromerisation" evidence="3">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="329" mass="37164" checksum="FF59FE1BE043FB49" modified="2016-10-05" version="1">MSCPLDSQPILTITTSKNWVLPPRPKNVRKSKPSLEKKKVKQQTARAQNAPLCPGKVAEVKKERLQIAHPCPAPLIPNSASSSALDSPDELAARIQIINNENYQLKTKLLLLIHDYKSLKSLVTSPAIPETPPVEILYDTTTTARKRVYPEICDDMNGLISDMSGLSHASPHSVVSPIDQELDQSGFEMELFNFINLEQDPLENIGEEYEAELRDQDEDDEEDSAALSRLLSPTSDSDENLLMTTLTRSTTVSTTNSVMEKKPFSQNFKFHDLPEFSEEDYAFLFDKSFSPEKMMSVIEEDQYNQVADFLEEKLMSNDVQYYVEKNHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI04</accession>
    <name>A0A1A0HI04_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein L6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_64312</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713993.1">
        <property type="nucleotide sequence ID" value="XM_018858342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI04"/>
    <dbReference type="STRING" id="869754.A0A1A0HI04"/>
    <dbReference type="GeneID" id="30031318"/>
    <dbReference type="OrthoDB" id="10252633at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000003">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000004">
        <property type="entry name" value="60S ribosomal protein L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="12"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="4">
        <location>
            <begin position="97"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="191" mass="21383" checksum="8B9F16887E4DE488" modified="2016-10-05" version="1">MKYIQTDQILEIPEGVTVNIDARSIKVTGPRGVLTKDLKHIDVTFAKVSDKAIKITVHNGDRKHVAALRTVKSLIANLITGVTKGYKYKMRYVYAHFPINVNVVDVDGEAFVEIRNFLGEKRVRQVKIFEGVTVEQSANQKDELVLSGNSLEAVSQNAADIQQICRVRNKDIRKFLDGIYVSERGLIDAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI06</accession>
    <name>A0A1A0HI06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_113223</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714275.1">
        <property type="nucleotide sequence ID" value="XM_018853957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI06"/>
    <dbReference type="STRING" id="869754.A0A1A0HI06"/>
    <dbReference type="GeneID" id="30026933"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF157">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="329" mass="37774" checksum="73DBBF52B3C3EB52" modified="2016-10-05" version="1">MDKILQLFLNEPVFQIGWPSVERPFGLHLWPLFSAAFEKFTGYPAENFEFVYNDTFLASGQLALGMIALYYVVIFGGRYVTSKLGVPVLKLNLLFQAHNLGLTVVSLVLLMLVLEQLVPMLYHHGLLYAICHRDAFSAPLVTLYYLNYLTKYWELLDTVFLVLRKKKLLFLHVYHHGATALLCFTQLMGSTSIEWVPITLNLGVHVVMYWYYFLSSRGIRVWWKEWVTRFQIIQFVLDLGFVYFAMYTETAHRHFDWLPNVGHCHGSMISAYCGLLILSSYLVLFISFYIHMYRKSSKKAAEKKRADAAATGKASGVDASSGKARSRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI93</accession>
    <name>A0A1A0HI93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Maf-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714370.1">
        <property type="nucleotide sequence ID" value="XM_018856756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI93"/>
    <dbReference type="STRING" id="869754.A0A1A0HI93"/>
    <dbReference type="GeneID" id="30029732"/>
    <dbReference type="OrthoDB" id="10267058at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="BIFUNCTIONAL DTTP/UTP PYROPHOSPHATASE/METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213:SF5">
        <property type="entry name" value="BIFUNCTIONAL DTTP_UTP PYROPHOSPHATASE_METHYLTRANSFERASE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="210" mass="23316" checksum="ED3D01B2FEC1F410" modified="2016-10-05" version="1">MWSSDSLQQLQTYEFILGSSSPRRAEILNSNIGVTKYTIMTSSFEENLPKSKISDVEYVTSTAQHKIPSIISQLETSKDYVLLVADTIVSCGGKVFEKPVSPEDQWAMLSHYRNHFSDIRVITAVHVCRVSGGQIVCHEFGHEITALEFDAKLTDLQLHYYIATGEGSNVAGGFKYQEKGSMLFVGLSGDYFNVVGLPVKKTHSLLTKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIG6</accession>
    <name>A0A1A0HIG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HECT-type E3 ubiquitin transferase TOM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_36571</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714272.1">
        <property type="nucleotide sequence ID" value="XM_018856760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIG6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIG6"/>
    <dbReference type="GeneID" id="30029736"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000004">
        <property type="entry name" value="E3 ubiquitin-protein ligase HUWE1, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="13">
        <location>
            <begin position="449"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="752"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="785" mass="90535" checksum="F7C8F107C9962346" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH2</accession>
    <name>A0A1A0HIH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9886</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714119.1">
        <property type="nucleotide sequence ID" value="XM_018859479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH2"/>
    <dbReference type="GeneID" id="30032454"/>
    <dbReference type="OrthoDB" id="1611972at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="389" mass="43906" checksum="BAF0156BCCA41FE4" modified="2016-10-05" version="1">MSHRKYEAPRHGSLGFLPRKRASSHRGKVKSFPKDIKSKPVALTAFLGYKAGMTTIVRDLDRPGSKMHKREVVEAATVVDTPAMVVVGVVGYVETPRGLRSLTTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYSAKYAQNADEINRELARITKYASVVRVLVHTQIKNTPLSQKKAHLAEIQINGGSTEDKVNWAKEHFEKTVTVDSVFEQNEMIDVVAVTKGHGFEGVTHRWGTKKLPRKTHRGLRKVACIGAWHPANVNWTVARAGQNGYHHRTSINHKVYRVGSGADEANGATEFDRTKKTINPLGGFVRYGNVKNDFVLLKGSIPGVRKRIVTLRKSLYVDTSRRALEKVNLKWIDTASKFGKGRFQTPAEKRAFMGTLKKDLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIP5</accession>
    <name>A0A1A0HIP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SRP9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714357.1">
        <property type="nucleotide sequence ID" value="XM_018853681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIP5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIP5"/>
    <dbReference type="GeneID" id="30026657"/>
    <dbReference type="OrthoDB" id="5419752at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039432">
        <property type="entry name" value="SRP9_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05486">
        <property type="entry name" value="SRP9-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP9" evidence="2">
        <location>
            <begin position="26"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="134" mass="14918" checksum="3D38F2603D0EBE40" modified="2016-10-05" version="1">MTKSNNKNRDAAKSAPLKKKNSNPALEEFIYNASDLLEAFPSALVSISYTVKENQENTGVKFTIRHSGQSKYLTYESFDTKEISRILAFIGPGGVTADSVNSEKKKSDPEEIEKKQDRITGLTSIMNNHDFHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA0</accession>
    <name>A0A1A0HJA0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Inhibitor I9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_34250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protease inhibitor I9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714715.1">
        <property type="nucleotide sequence ID" value="XM_018856454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA0"/>
    <dbReference type="GeneID" id="30029430"/>
    <dbReference type="OrthoDB" id="5518345at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004866">
        <property type="term" value="F:endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052471">
        <property type="entry name" value="PBI_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28288:SF2">
        <property type="entry name" value="PROTEASE B INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="2">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="73" mass="8134" checksum="37285B49CFD25AA6" modified="2016-10-05" version="1">MSSKNHIVTLKETASTEDVNSLKLKIAEQGGVILDEFSLIKGFSVKLTQPVADFIKNHHLVNSIEEDKEVKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJE7</accession>
    <name>A0A1A0HJE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76880</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGA52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714444.1">
        <property type="nucleotide sequence ID" value="XM_018858602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJE7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJE7"/>
    <dbReference type="GeneID" id="30031578"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018807">
        <property type="entry name" value="YJL171C/Tos1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF3">
        <property type="entry name" value="CELL WALL PROTEIN YJL171C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10290">
        <property type="entry name" value="YJL171C_Tos1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 N-terminal" evidence="9">
        <location>
            <begin position="31"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="8">
        <location>
            <begin position="103"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="41379" checksum="F201F4F3124B886F" modified="2016-10-05" version="1">MAMFLKCLGATQGISGLQGRSASSSNSAVDTIAFENVGFTGYYYDVQEILNADSEDCSCTLSDSFTVFEGSNAPLDEELSIHFRGPLSLWLFGAYTADDSSSGTWDRVSYYDSNSQASTNITFLTHAGESSTCLGSALTFASGNGTGYADSSTILESNNLINSNEEFIIFSGLACPDSGFDNSCGIYRSGIPAYHGFSGTTKMFLFEFEMPEANVTLEYYYNMPAIWFLNAKIPRTSQYGSDASCSCWNSGCGEIDIFEVMNSSTTGQLYATIHDYQGTGDINTGMQADGYFSRDVSSTMKGGVVFGTDGTISIFMLDSLEISESLDASSVASWISTADEVVDTLSSVTMNTSTTASKSSNGVMSISQSRSAAFMSFVFLLFMYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF4</accession>
    <name>A0A1A0HJF4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_16404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714498.1">
        <property type="nucleotide sequence ID" value="XM_018854638.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJF4"/>
    <dbReference type="GeneID" id="30027614"/>
    <dbReference type="OrthoDB" id="1749473at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF11">
        <property type="entry name" value="RNA-BINDING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="52"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="133" mass="14838" checksum="24C7385DF0E970F8" modified="2016-10-05" version="1" fragment="single">RKKTYNLKSSDRRKASGVRAEAFLHSTQKNKTGKLAPARKPADKDVWDPKHFRLFVGNLGDDATDDILAAAFSKYASVSKVRVPIDAKTRTNRGYGFVAFASADDYLKAFKEMNGKYVGLRPVQLKRAESKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJL3</accession>
    <name>A0A1A0HJL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2 8">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_10255</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2 8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714558.1">
        <property type="nucleotide sequence ID" value="XM_018853723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJL3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJL3"/>
    <dbReference type="GeneID" id="30026699"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006390">
        <property type="term" value="P:mitochondrial transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="10">
        <location>
            <begin position="313"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1138" mass="128047" checksum="6615578A53636DBC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJP0</accession>
    <name>A0A1A0HJP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycoside hydrolase family 38 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_30558</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714716.1">
        <property type="nucleotide sequence ID" value="XM_018855661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJP0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJP0"/>
    <dbReference type="GeneID" id="30028637"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034270">
        <property type="term" value="C:Cvt complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019309">
        <property type="term" value="P:mannose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000002">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="5">
        <location>
            <begin position="548"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="818" mass="93649" checksum="7F5F4EA2AC6C05FF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJX4</accession>
    <name>A0A1A0HJX4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714594.1">
        <property type="nucleotide sequence ID" value="XM_018855640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJX4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJX4"/>
    <dbReference type="GeneID" id="30028616"/>
    <dbReference type="OrthoDB" id="4091406at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="238" mass="27049" checksum="1E2BC919B10E60FB" modified="2016-10-05" version="1">MALLVGPYQRGFIQDRRSNQNPTESPIMVELMTKSRGLRNSSQYQAVLMADFTEASDRNSHQYMEAVLKKMGIGTAMNYAKAAAIVSNYEKVSNSFLSACKPELLGFAPHFPHPSQDTLPFPQAYIHVDNTVYLGKPLKAVDWMNSIKNLPFQAFQLGYSLLALIKRAQGTVQFIFSKPVYPDIVKCMTSKELQAWYQMRVCRHQKRKTIYTRPNYGGYGLIKIKIQLQGHRIQGTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKG1</accession>
    <name>A0A1A0HKG1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Branched-chain-amino-acid aminotransferase</fullName>
            <ecNumber evidence="16">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_36247</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-oxocarboxylate + L-methionine = 4-methylsulfanyl-2-oxobutanoate + an L-alpha-amino acid</text>
            <dbReference type="Rhea" id="RHEA:31763"/>
            <dbReference type="ChEBI" id="CHEBI:16723"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:31765"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 6/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="16"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714863.1">
        <property type="nucleotide sequence ID" value="XM_018856710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKG1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKG1"/>
    <dbReference type="GeneID" id="30029686"/>
    <dbReference type="OrthoDB" id="1732691at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010326">
        <property type="term" value="F:methionine-oxo-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009083">
        <property type="term" value="P:branched-chain amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01557">
        <property type="entry name" value="BCAT_beta_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000004">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000005">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005786">
        <property type="entry name" value="B_amino_transII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033939">
        <property type="entry name" value="BCAT_family"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01123">
        <property type="entry name" value="ilvE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009897">
        <property type="entry name" value="PRK13357.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825:SF44">
        <property type="entry name" value="BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11825">
        <property type="entry name" value="SUBGROUP IIII AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006468">
        <property type="entry name" value="BCAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 16" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10 16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 15" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="13">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006468-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA24382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="371" mass="40993" checksum="5CA30B0BFA4078EF" modified="2016-10-05" version="1">MSQAPLDASKLVITKTKSPKEKIPNDQLVFGKTFSDHMLEVEWTSENGWAAPTITPYHNLSFDPSTIVFHYGFEAFEGMKAYRDKQNKIRIFRPNKNMARLNASADRICLPTFDGEQFTKLIEEFVKIDQDFVPQGEGYSLYLRPTIIGTSATLGVSTPDKALLYLIASPVGPYYSTGFKAVSLEATDYAVRAWPGGVGNRKLGANYAPCVKPALEAAKRGYQQNLWLFGEEGYITEVGTMNVFFVFQDASGKKELVTAPLDGTILEGVTRDSVLGLARAKLPSSEWTISERKFTISEVKERASKGELVEAFGSGTAALVSPIKNIGWKGEDISVPLAKGEAGELTVQVADWIKKIQYGEEEFEGWSRVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKM9</accession>
    <name>A0A1A0HKM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit</fullName>
            <ecNumber evidence="5">2.4.1.198</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GlcNAc-PI synthesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_10586</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.198" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714928.1">
        <property type="nucleotide sequence ID" value="XM_018853806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKM9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKM9"/>
    <dbReference type="GeneID" id="30026782"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03796">
        <property type="entry name" value="GT4_PIG-A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000053">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase GPI3 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000026">
        <property type="entry name" value="Phosphatidylinositol N-acetylglucosaminyltransferase subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039507">
        <property type="entry name" value="PIG-A/GPI3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="400"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="14">
        <location>
            <begin position="44"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="13">
        <location>
            <begin position="195"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA24447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="443" mass="49240" checksum="710C9CAE7E5961DD" modified="2016-10-05" version="1">MARSLNIAMVTDFFYPQPGGVEFHVYHLSQKLIDLGHSVVIVTHNYGNRTGIRMLTNGLRVYYVPLAQLYRSSTFPTLLVGFPTLRTIFIREQIDIVHGHGSYSVMGAEGLLHGRTMGLKTVFTDHSLFGFADAQSIVGNKILKFTLSDIGHVICVSHTCKENTVLRGSLDPLKVSVIPNAVVSEDFAPEYEKKSEGGRITIVVISRLFPNKGADLLTAVVPHICHARSNVDFLIAGDGPKFLDLEQMREKHSLQDRVTLIGAVKHEEVRNVMVKGDIYLHPSLTEAFGTVLVEAASCGLYVVTTKVGGIPEVLPQHMTSFAEPEEDSLVENLFKAIDHVSSGEIDTSLFHKEVASMYSWKDIAKRTENVYLSLADTELHQLLLTRLLKFYFCGAVAGKLYLLIVVVDIFILMILDWFFPAEHIDLASKWPRNTASTPEVAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HL41</accession>
    <name>A0A1A0HL41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34561</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715009.1">
        <property type="nucleotide sequence ID" value="XM_018856488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HL41"/>
    <dbReference type="STRING" id="869754.A0A1A0HL41"/>
    <dbReference type="GeneID" id="30029464"/>
    <dbReference type="OrthoDB" id="3477330at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050675">
        <property type="entry name" value="OAF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069:SF32">
        <property type="entry name" value="ARGININE METABOLISM REGULATION PROTEIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31069">
        <property type="entry name" value="OLEATE-ACTIVATED TRANSCRIPTION FACTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="6">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="241"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="833" mass="94972" checksum="EA821F27A0C8AB12" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ53</accession>
    <name>A0A1A0GZ53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">MICOS complex subunit MIC60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitofilin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_33879</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a role in keeping cristae membranes connected to the inner boundary membrane. Also promotes protein import via the mitochondrial intermembrane space assembly (MIA) pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MICOS complex subunit Mic60 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709276.1">
        <property type="nucleotide sequence ID" value="XM_018856416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ53"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ53"/>
    <dbReference type="GeneID" id="30029392"/>
    <dbReference type="OrthoDB" id="10261039at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061617">
        <property type="term" value="C:MICOS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042407">
        <property type="term" value="P:cristae formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019133">
        <property type="entry name" value="MIC60"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415:SF7">
        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15415">
        <property type="entry name" value="MITOFILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09731">
        <property type="entry name" value="Mitofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="300"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA16977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="581" mass="64106" checksum="5DDDFE279FEC3E55" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1S6</accession>
    <name>A0A1A0H1S6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutathione S-transferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_226484</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709379.1">
        <property type="nucleotide sequence ID" value="XM_018855179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1S6"/>
    <dbReference type="STRING" id="869754.A0A1A0H1S6"/>
    <dbReference type="GeneID" id="30028155"/>
    <dbReference type="OrthoDB" id="2098326at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03046">
        <property type="entry name" value="GST_N_GTT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051:SF9">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44051">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14497">
        <property type="entry name" value="GST_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="96"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA17984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="28415" checksum="D9CA5BC514C52ADD" modified="2016-10-05" version="1">MAQDRIVLHYLKDSRASRIVFLFEELGLEYEIKAYEWTPELVAPPELKLVWPLGLSPAVQIFKADDPEPIVLAESGQIVQFILNNYDSEGKFKPESMRDQVLVDYYLHFAEGTLEPHIVSLLVGYVASQRAPFGFGWLVKLVLSQINSQFYLRKLKTALAFLNSQLEAKDGGYFVGNKLSGADFILDFPINENLFLNPYKVKRLGIDIDPSKDYPRLVEWNKLITERPLHVKALEKKASVMKNKRGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Z5</accession>
    <name>A0A1A0H1Z5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Nicotinate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 9">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_47747</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + nicotinate + ATP + H2O = nicotinate beta-D-ribonucleotide + ADP + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the NAPRTase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="3 9"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709370.1">
        <property type="nucleotide sequence ID" value="XM_018858215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Z5"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Z5"/>
    <dbReference type="GeneID" id="30031191"/>
    <dbReference type="OrthoDB" id="193380at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.140.10:FF:000009">
        <property type="entry name" value="Nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.140.10">
        <property type="entry name" value="nicotinate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00570">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006406">
        <property type="entry name" value="Nic_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01514">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098:SF1">
        <property type="entry name" value="NICOTINATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nicotinate phosphoribosyltransferase N-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Nicotinate/nicotinamide phosphoribosyltransferase" evidence="10">
        <location>
            <begin position="167"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA17975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="422" mass="47908" checksum="6EEBBA8AA92EC8F7" modified="2016-10-05" version="1">MVHHSAIKSLLDTDLYKISMQAAVHKHFRDVPVSYRYKNRTRQLSLNRAAISWLKSQILALGELRFLPDEISYLRETLPMLPREYLDSLKAFKLDPPSQVKYYNDEKDLDEFYLEISGLWYETIPYEIHILSLVSEAYFKFVDTEWNYDGQYDIAAEKTRQLLSNNCAFSEFGTRRRRLGEAQDIVVRAIMETAQEMGPEKARLFLGTSNVAYARKYGVAPIGTVAHEWFMGVASITQDYTNANKTAMEYWLQTFGPDHCGLALTDTFGTDAYLKNFTKPYTDYYTGVRQDSGDPELYCHKIAAHFKALGYPAYSKTICFSDSLNIGKCLKYKKCADQDGLKVSFGIGTFFTNDFSSTGAQGGKSPPLNIVIKMASAAGNPSIKISDNIGKNIGDAGVVRRVKEELGYTEKEWKEGDETNRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4T9</accession>
    <name>A0A1A0H4T9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">DNA topoisomerase 2</fullName>
            <ecNumber evidence="5 16">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_79938</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 16">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the type II topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5 16"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709500.1">
        <property type="nucleotide sequence ID" value="XM_018859151.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H4T9"/>
    <dbReference type="GeneID" id="30032127"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 15 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 16" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="19">
        <location>
            <begin position="468"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="20">
        <location>
            <begin position="722"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1212"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1157"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1231"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1304"/>
            <end position="1314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1315"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1332"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="1373"/>
            <end position="1398"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="15">
        <location>
            <position position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA18965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1398" mass="159273" checksum="A632F06546B1F439" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H520</accession>
    <name>A0A1A0H520_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709542.1">
        <property type="nucleotide sequence ID" value="XM_018858131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H520"/>
    <dbReference type="STRING" id="869754.A0A1A0H520"/>
    <dbReference type="GeneID" id="30031107"/>
    <dbReference type="OrthoDB" id="4073665at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="104" mass="11527" checksum="C98D9BEFD7264ED2" modified="2016-10-05" version="1">MFSRISSRAGRVARRFQSHAAQAEAKPAPFNNKYNFNINPPPVHAYWNARNFSVLIAFVPVFLGAGYLGKYAGENAGGFEALLDFADSEQSPMKELKFGEAQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H538</accession>
    <name>A0A1A0H538_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_75076</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NFX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709557.1">
        <property type="nucleotide sequence ID" value="XM_018858427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H538"/>
    <dbReference type="STRING" id="869754.A0A1A0H538"/>
    <dbReference type="GeneID" id="30031403"/>
    <dbReference type="OrthoDB" id="6512771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06008">
        <property type="entry name" value="NF-X1-zinc-finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034078">
        <property type="entry name" value="NFX1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001374">
        <property type="entry name" value="R3H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000967">
        <property type="entry name" value="Znf_NFX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12360:SF12">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR NF-X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01424">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01422">
        <property type="entry name" value="zf-NF-X1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00393">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00438">
        <property type="entry name" value="ZnF_NFX"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51061">
        <property type="entry name" value="R3H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="R3H" evidence="11">
        <location>
            <begin position="491"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="701"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="722" mass="81245" checksum="28D549E4E68BD19B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H574</accession>
    <name>A0A1A0H574_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">EGC1</name>
        <name evidence="7" type="ORF">METBIDRAFT_60496</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709718.1">
        <property type="nucleotide sequence ID" value="XM_018858311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H574"/>
    <dbReference type="STRING" id="869754.A0A1A0H574"/>
    <dbReference type="GeneID" id="30031287"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000130">
        <property type="entry name" value="Endoglucanase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF13">
        <property type="entry name" value="PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="6">
        <location>
            <begin position="69"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="476" mass="55553" checksum="3DE57C3A3428AF41" modified="2016-10-05" version="1">MGFLKVVGTKIVNANEEPVVLKGTATGGHLNMENFITGYPGHETEHKLVLERKMGTEKYKFYFDKFYEYFWTDSDAKFFAEELKLNCLRIPFNYRHFLDDSGDMFKINPKGFELVDRIVDSCARHGIYTILDLHTAPGGHNQDWHSDSGIHKTLFWDFKVFQDIVLNLWAKLAKHYKDNEWVAGYNPLNEPAVEDHSKLVSYYEKLEKTIRAVDANHILFLDGNTYAADFRQFPVPTWPNTVYSIHDYTVFGFPGADPMYEGKPEQLAKLRSSYERKIEYMKKHHVPVWNGEFGPVYASHFRGDADPDSINKSRYRVLRDQLGIYKQGDPSGDNTPISWSIWLWKDIGYQGMLHVAPESKFYQIFGDWLHKKRALGLDRWGNDIDPQNGELYDGLLGHFKTNVPEKYHKALYPNTWTMKDYIARVARDMLFSQYAQEEYADLYAELTFDDLDELAACFKFENCVKRKELNDILTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5V1</accession>
    <name>A0A1A0H5V1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipid scramblase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_79822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phospholipid scramblase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709825.1">
        <property type="nucleotide sequence ID" value="XM_018859132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5V1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5V1"/>
    <dbReference type="GeneID" id="30032108"/>
    <dbReference type="OrthoDB" id="191150at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017128">
        <property type="term" value="F:phospholipid scramblase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903147">
        <property type="term" value="P:negative regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005552">
        <property type="entry name" value="Scramblase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248:SF9">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23248">
        <property type="entry name" value="PHOSPHOLIPID SCRAMBLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03803">
        <property type="entry name" value="Scramblase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="325" mass="37539" checksum="C796C0D63070C88D" modified="2016-10-05" version="1">MPRFSPYQSPGAQTLRTRQTEEISAEDIRRYKESRARRVKRSFFYHESPSSDSHQFENDPSQFRTVFEMPPNENGIITPEDGIYSILKEPTLVIERQVEFMNVILGFEQANKYKIMNSLGEQIGYMEEKDYGILKMIGRQFFRLHRPFDIDVFNNYGDLLLTIKRPFSFINSHIKCLLPGFDTDGNLMHEVVGESVQSWHLWRRKYNLFKLDDETTDEYNQFGLIDSPFLAFDFPVKNEEGDVIASVDRNWVGLGRELFTDSGVYIIRMDPASFAGLGDLYPSVAGPLTLDQRAILLGNAVSIDFDYFSRHSRQGGGFMSFGSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5X8</accession>
    <name>A0A1A0H5X8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33536</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709892.1">
        <property type="nucleotide sequence ID" value="XM_018856323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5X8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5X8"/>
    <dbReference type="GeneID" id="30029299"/>
    <dbReference type="OrthoDB" id="440385at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="96"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="247" mass="26856" checksum="AFBC4C9AB56DB610" modified="2016-10-05" version="1">MAYYQQDPNLQFYQSSYGNTYQQPAMDSANMGTMNSGFASNFDVSGDMGGMDLTPGLLSAFGTSGYSGEPPLLEELGINFQHMKVKTLAVLNPVNRDISPDIMADSDLAGPILFVLLFGTLLLLSGKVQFGYVYGVGLFGIVSLHYLFKLMSNDVQIDVTRSASILGYCLLPLVFICALGLVVSLDSMFGYIASGLAVFWCTYSASGLFVTVLKLHNVRPLIAYPLCLFYLVFALMAIFVENKPISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H624</accession>
    <name>A0A1A0H624_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">3-ketodihydrosphingosine reductase TSC10</fullName>
            <ecNumber evidence="10">1.1.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">3-dehydrosphinganine reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">KDS reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_18602</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the reduction of 3'-oxosphinganine (3-ketodihydrosphingosine/KDS) to sphinganine (dihydrosphingosine/DHS), the second step of de novo sphingolipid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>sphinganine + NADP(+) = 3-oxosphinganine + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.102"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:22642"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.102" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710009.1">
        <property type="nucleotide sequence ID" value="XM_018854801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H624"/>
    <dbReference type="STRING" id="869754.A0A1A0H624"/>
    <dbReference type="GeneID" id="30027777"/>
    <dbReference type="OrthoDB" id="10267115at2759"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08939">
        <property type="entry name" value="KDSR-like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045022">
        <property type="entry name" value="KDSR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="286"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="17">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00026241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA19481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="315" mass="34794" checksum="A460577209A32609" modified="2016-10-05" version="1" fragment="single">MWFSKSHFKPEGKTALVVGASQGVGADLGLRLFEKGCSVVLVARTEAKLKQQVARILDENKKAQKALRPKAKEPTVSYQVCDVADYEACEHMWHELLVEKHVDPDFIFCCAGLSIPKLFGDLTGRELASGMSTNYLTAVNTIHAGHKSILQMNVDADPAGFKPRHVVLFSSTVASFPFIGYGQYAPCKAALMSLSVILRQELVSYNYRVSCVFPGNFESEGYAEEQKTKPEITRKIEGPSVAIPSMECCDIVLDRLAKGYDSIYTDFIGWVLASSALGVMPRCWGFLQILVSLIFLVIAPIANMFIYLDIKASFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H636</accession>
    <name>A0A1A0H636_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="21">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">METBIDRAFT_79560</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 21">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 21">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="18">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 21">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 21">Mitochondrion inner membrane</location>
            <topology evidence="1 21">Peripheral membrane protein</topology>
            <orientation evidence="1 21">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="21"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710077.1">
        <property type="nucleotide sequence ID" value="XM_018859089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H636"/>
    <dbReference type="STRING" id="869754.A0A1A0H636"/>
    <dbReference type="GeneID" id="30032065"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000482">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:001062">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 21" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 18 21" id="KW-0274">FAD</keyword>
    <keyword evidence="6 18 21" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 21" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 21" id="KW-0813">Transport</keyword>
    <keyword evidence="5 21" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="22">
        <location>
            <begin position="44"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="23">
        <location>
            <begin position="494"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="49"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="438"/>
            <end position="439"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="19">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR630664-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OBA19549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="629" mass="68856" checksum="AE038397C12DED90" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H637</accession>
    <name>A0A1A0H637_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSm2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33114</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of LSm protein complexes, which are involved in RNA processing and may function in a chaperone-like manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709948.1">
        <property type="nucleotide sequence ID" value="XM_018856228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H637"/>
    <dbReference type="STRING" id="869754.A0A1A0H637"/>
    <dbReference type="GeneID" id="30029204"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000009">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016394">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="2"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="96" mass="10808" checksum="7D0D8952E683D90C" modified="2016-10-05" version="1">MLFFSFFKTLVDQEVVVELKNDLEIKGTLKSVDQFLNLKLDNISTVNEGKYPHLKAVKTLFVRGSTVRYVHLSASSVDCTLLQDASRREAMVQGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6A7</accession>
    <name>A0A1A0H6A7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_230489</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709976.1">
        <property type="nucleotide sequence ID" value="XM_018855252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6A7"/>
    <dbReference type="GeneID" id="30028228"/>
    <dbReference type="OrthoDB" id="10676409at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="439" mass="50929" checksum="171D557161910765" modified="2016-10-05" version="1" fragment="single">GPHLSLNPFCIRILASKSQTYGQFQTLIKSVFETVNTKKSYEKKNNQKNCPSRNNCFRTSMLNLKDASNKRHFVNQNLRNGSHVEFLFENQKSLNTEPGVHLGEVDQEYVRDIDKYLLAVEGGEDQGREFSPRRESRKNLRSTPVWTRAKLLLLQFQEETDIVSGMQSNEPPAMQQKPCSSDSQYFHFPENYHSSHPTSTLESTDIFRVINDINFRVESDSASIILKEIVDNYRYIFGHILKIVVVPCNIKIQGFLGDILSIRNTFGELLSLGLGSSNELLIIYEHLKNPKETDVKFWEEKMERSSSKVTEICIHMEKIQCDLAFLETWFNLRVSRITDTVAKISIMRDKVLESYYRFYERNSSEIFFCSTDVHKYAGVDEYKKKTLWHYNLDFEDFKDIMSQSLQALIASSETVDMIIERLHDVITDAKILNENNCSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6G5</accession>
    <name>A0A1A0H6G5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_215004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710031.1">
        <property type="nucleotide sequence ID" value="XM_018855083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6G5"/>
    <dbReference type="GeneID" id="30028059"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="136" mass="15255" checksum="F80E3DAC8A656CB3" modified="2016-10-05" version="1">MFNSAKLPKNSAMKMCPTIRRLACSSRVLLLCLLIAVHLSVSCISCCICLCHPEFPFILGRIRVCRLVLNCDRGLRVCELASRQFLRGAAPGSHFLSGCASPRNVDFLPIKLWSICWPFISSLSYIPAQLWGVHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S1</accession>
    <name>A0A1A0H6S1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pal1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_206678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710251.1">
        <property type="nucleotide sequence ID" value="XM_018855000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S1"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S1"/>
    <dbReference type="GeneID" id="30027976"/>
    <dbReference type="OrthoDB" id="5352132at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013226">
        <property type="entry name" value="Pal1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28307">
        <property type="entry name" value="PROTEIN PAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28307:SF2">
        <property type="entry name" value="PROTEIN PAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08316">
        <property type="entry name" value="Pal1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="412" mass="44908" checksum="122F69CE974F7E41" modified="2016-10-05" version="1">MYHQNNLLYDSVGRRLSTNPFRQIGAQGAVLSTPLSNSAFDEWVQKNRGLMSDQSDEEEQQAFQRPSFPSQTRTGSDTNVNYSRTMSSNNPFASAMNGEYKHHRDPPPPPPAQATRLKSPSKDIKSPQKMVPPPPSYEEAAGPKAQSKTYPKEKESLKLSLRSSRPHRSHSDSAKHKERKSRHKSKESKKKAVAPHQPKNLDVIDKLDVSAFFGGRFHHDGPFDACAPHRNKNVKAAPVMAFPADGPNNSMKANAGPKDNIELAFGNIEDHNMIIGRSARLHSATKGSPQAKPFGTQGDIPRLNPSVVAFDAHEKAAPIHGFSTAGLGSTTFLNGAPAPKLDDSSSSPSANKGGLGRKKSLAQRLRKNSGSDVTARRGSNDSSTEYRSSSFEEEEGSSSLLLRVRSLKVGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H706</accession>
    <name>A0A1A0H706_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein Zds1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710265.1">
        <property type="nucleotide sequence ID" value="XM_018854266.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H706"/>
    <dbReference type="GeneID" id="30027242"/>
    <dbReference type="OrthoDB" id="5589766at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040206">
        <property type="entry name" value="Zds1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013941">
        <property type="entry name" value="ZDS1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28089">
        <property type="entry name" value="PROTEIN ZDS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28089:SF1">
        <property type="entry name" value="PROTEIN ZDS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08632">
        <property type="entry name" value="Zds_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01327">
        <property type="entry name" value="Zds_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein Zds1 C-terminal" evidence="2">
        <location>
            <begin position="1179"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="530"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="830"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="863"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="830"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="872"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="882"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="923"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="948"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="989"/>
            <end position="1002"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1114"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1293" mass="143166" checksum="95DC107DE3F2C9E2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H745</accession>
    <name>A0A1A0H745_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ras-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_32856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endomembrane system</location>
            <topology evidence="3">Lipid-anchor</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710310.1">
        <property type="nucleotide sequence ID" value="XM_018856146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H745"/>
    <dbReference type="STRING" id="869754.A0A1A0H745"/>
    <dbReference type="GeneID" id="30029122"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01861">
        <property type="entry name" value="Rab6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000823">
        <property type="entry name" value="Small GTPase RAB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="22817" checksum="70547295720B3CDB" modified="2016-10-05" version="1">MSDKTFLRKYKIVFLGDQSVGKTSLITRFMYDTFDDQYASTIGIDFLSKTMYLEDNKTVRLQLWDTAGQERFRSLIPSYIRDSHVAVVCYDITSKKSFTSLEKWISDVRMERGEDVIVVVVGNKSDLNAKRQVSSEEATKFASEAGAKFFIETSTKANHNVKLLFKKIASCLPEFKESAEPEAPAETVNISIESAAPLSSSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H764</accession>
    <name>A0A1A0H764_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">orotate phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_45042</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710268.1">
        <property type="nucleotide sequence ID" value="XM_018857865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H764"/>
    <dbReference type="STRING" id="869754.A0A1A0H764"/>
    <dbReference type="GeneID" id="30030841"/>
    <dbReference type="OrthoDB" id="5553476at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046132">
        <property type="term" value="P:pyrimidine ribonucleoside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000008">
        <property type="entry name" value="Orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46683:SF1">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="9">
        <location>
            <begin position="53"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="219" mass="23396" checksum="934B22000C642D41" modified="2016-10-05" version="1">MQSFQTSFLDLALESQALKFGQFTLKSGRQSPYFFNLGLFNTGKLLSTLASAYAHAIIASGIEFDILFGPAYKGIPLAAITVAKLAEIDPKKYGNTGYSFNRKEKKDHGEGGSIVGCDLTGKKVLIIDDVMTAGTAINEAFTIIGAEKGDVVGCIIALDRQETTADSHLSATQVVSVKYGIPVSSIVSLSDIISYLADKLSPENLAAIKEYRAQYAPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7A4</accession>
    <name>A0A1A0H7A4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710302.1">
        <property type="nucleotide sequence ID" value="XM_018856144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7A4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7A4"/>
    <dbReference type="GeneID" id="30029120"/>
    <dbReference type="OrthoDB" id="433955at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019410">
        <property type="entry name" value="Methyltransf_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14614:SF156">
        <property type="entry name" value="PROTEIN-LYSINE N-METHYLTRANSFERASE EFM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10294">
        <property type="entry name" value="Methyltransf_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="414" mass="46814" checksum="B4F40CFF90447EB2" modified="2016-10-05" version="1">MDFDPLELFTPTSSSFTTSISKIDTSSNHLNSARLVASSDKSRPDEKCGADEDEHIDEEDGFLPIHIHDLPLLRCLPPLKVLISFLRLLSPETSLNFAPISHTHREDTRRIDPLLVLAKKNVDMDVDAWLWLQKFCPRFGTETRLADLPLLADSLKKTFSSQYNAWLTQLISCEFAWISQDESTEIQTLAALRIAENCGRTAQPEMLRAIEIPMLPTKLLLKEPSLTSDNLGLKTWGSSFVLGSRLALQTHKEYLLDPVLELGAGTGLVGMVACCLGHTTMLTDLAEIVPNLQNNIELNGIENARVAELDWSNPKSFLQRFGNVKYSTIILSDPLYSSHHPQWIVNMLNLFLSQEKDSRVLLQVPVRRTFEKERASLWRLMEENGYLAAEECEELGYDDFGESSFIFKKYIRAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7C3</accession>
    <name>A0A1A0H7C3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetyl-coenzyme A synthetase</fullName>
            <ecNumber evidence="6">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_44561</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710322.1">
        <property type="nucleotide sequence ID" value="XM_018857807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7C3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7C3"/>
    <dbReference type="GeneID" id="30030783"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016880">
        <property type="term" value="F:acid-ammonia (or amide) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019654">
        <property type="term" value="P:acetate fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05966">
        <property type="entry name" value="ACS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000004">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011904">
        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="10">
        <location>
            <begin position="35"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="8">
        <location>
            <begin position="99"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="9">
        <location>
            <begin position="548"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="672" mass="74387" checksum="174B316DCD049962" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7G0</accession>
    <name>A0A1A0H7G0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710363.1">
        <property type="nucleotide sequence ID" value="XM_018857909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7G0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7G0"/>
    <dbReference type="GeneID" id="30030885"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015665">
        <property type="term" value="F:alcohol transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022890">
        <property type="term" value="F:inorganic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015850">
        <property type="term" value="P:organic hydroxy compound transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF34">
        <property type="entry name" value="PROTEIN HOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="405"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="61"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="587" mass="64668" checksum="FA2E10ACA03F2668" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H7</accession>
    <name>A0A1A0H7H7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Phosphatidylinositol transfer protein SFH5</fullName>
            <shortName evidence="16">PITP SFH5</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_44794</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Non-classical phosphatidylinositol (PtdIns) transfer protein (PITP), which exhibits PtdIns-binding/transfer activity in the absence of detectable PtdCho-binding/transfer activity. Regulates PtdIns(4,5)P2 homeostasis at the plasma membrane. Heme-binding protein that may play a role in organic oxidant-induced stress responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol)(out)</text>
            <dbReference type="Rhea" id="RHEA:38691"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:38692"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
            <topology evidence="2 16">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Microsome membrane</location>
            <topology evidence="16">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the SFH5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710372.1">
        <property type="nucleotide sequence ID" value="XM_018857840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7H7"/>
    <dbReference type="GeneID" id="30030816"/>
    <dbReference type="OrthoDB" id="75724at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046488">
        <property type="term" value="P:phosphatidylinositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017157">
        <property type="term" value="P:regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042938">
        <property type="entry name" value="Sfh5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47669:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SFH5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12 16" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0492">Microsome</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="18">
        <location>
            <begin position="165"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA19847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="38191" checksum="32AD360798609771" modified="2016-10-05" version="1">MSLVSSQELTLEQTLKLQELVEAVPQILAETDHPEYDEIYGYRIGIDGADHVDTAIRNEILLKFLIATDYDVAAAQQKVVNTLNWRREFQVLSAAYYEKFDAGLEKMGVITEYPGNADNFRVVTWNLYANLKNPKKLFAKYGVGVTDDGSAAAENDKNSVPGTMFLRWRVGLMERSLSLLDFTNKDNNKAAQVHDYYNVSMFRMDPGMKAATKEIIQVFGDNYPEVLSKKYFINVPLLMGWIFTFFKATGLMSAATIKKFEMQNHGDLSTSFGKDNLPKEYNGGHENKAVPDIFALATVDSKIVVPEYGAILLQKLKDKSSQEASSATRDTEVAEEITNAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7I5</accession>
    <name>A0A1A0H7I5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-splicing factor CLF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32908</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710388.1">
        <property type="nucleotide sequence ID" value="XM_018856159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7I5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7I5"/>
    <dbReference type="GeneID" id="30029135"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000354">
        <property type="term" value="P:cis assembly of pre-catalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="9">
        <location>
            <begin position="68"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="709" mass="81488" checksum="85AD18AD414B5319" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7P5</accession>
    <name>A0A1A0H7P5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DDHD-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_79355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710571.1">
        <property type="nucleotide sequence ID" value="XM_018859044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7P5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7P5"/>
    <dbReference type="GeneID" id="30032020"/>
    <dbReference type="OrthoDB" id="69269at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032048">
        <property type="term" value="P:cardiolipin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046337">
        <property type="term" value="P:phosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004177">
        <property type="entry name" value="DDHD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058055">
        <property type="entry name" value="PA-PLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055555">
        <property type="entry name" value="PA-PLA1_DUF7131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057826">
        <property type="entry name" value="WWE_C20G8.02"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509:SF10">
        <property type="entry name" value="LD21067P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23509">
        <property type="entry name" value="PA-PL1 PHOSPHOLIPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02862">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23465">
        <property type="entry name" value="DUF7131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23463">
        <property type="entry name" value="WWE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01127">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51043">
        <property type="entry name" value="DDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="652"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="DDHD" evidence="2">
        <location>
            <begin position="472"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="704" mass="80175" checksum="C113D11553A14638" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H864</accession>
    <name>A0A1A0H864_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710607.1">
        <property type="nucleotide sequence ID" value="XM_018857858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H864"/>
    <dbReference type="STRING" id="869754.A0A1A0H864"/>
    <dbReference type="GeneID" id="30030834"/>
    <dbReference type="OrthoDB" id="368909at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031417">
        <property type="term" value="C:NatC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06168">
        <property type="entry name" value="LSMD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034110">
        <property type="entry name" value="LSMD1_Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="84" mass="9395" checksum="BB88BCB14EC9E63D" modified="2016-10-05" version="1" fragment="single">TQMSVNKFVGTNLHIELVDGRLVEGVLAVVDPFGNLLLSNVYEIAQDKIKPSEIHRRELGLVSVPSDQLKDIFLDENQLHVLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H876</accession>
    <name>A0A1A0H876_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_44344</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710748.1">
        <property type="nucleotide sequence ID" value="XM_018857780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H876"/>
    <dbReference type="STRING" id="869754.A0A1A0H876"/>
    <dbReference type="GeneID" id="30030756"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="4">
        <location>
            <begin position="26"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="478" mass="53543" checksum="F56F9EDF7C65B03B" modified="2016-10-05" version="1" fragment="single">MGPESHVSIRSRPKTQKAKVSKPERKPKTSHNIIEKKYRTSINAKILELREVVPTLKFATGKARVLMADLDGLSPACKLNKASILTKANEYIKHMRKKNKTLEKQIRDLQEFVSRASAGQPIMDSSPVTPQPPMSTYTSYFEPVNTQTLFQRRVPQAQPIDEAIMNNYAPVSNMNPRTVASQHIQPQPYPNANMFFGGLATMMGSSYINDNNFQAMSALPVFPATLFGNFTTASLLLYTLRVFVAGLGLFMVVSSLVGNSLPKSRKSKSNNALENISRLFSFMSTHSREPLSEDEKCKITALLLGSNSAPITSWIRAYICLRTKSAGFETLLLQFLVGTVVIRRGSYLSQLVKLDLKLRKNALLAAEYTGSHQSLSKLARLIKNVDGLLMFDSESFSQRFYNLSHDLPLCRNVNNGENALLFVDSRLKNENNLYAIVFEWRVLEILHELTLVYLDLLVSPSGARDDGLEDLKQDSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8T7</accession>
    <name>A0A1A0H8T7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial 15S rRNA processing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32524</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Regulates mitochondrial small subunit maturation by controlling 15S rRNA 5'-end processing. Localizes to the 5' precursor of the 15S rRNA in a position that is subsequently occupied by mS47 in the mature yeast mtSSU. Uses structure and sequence-specific RNA recognition, binding to a single-stranded region of the precursor and specifically recognizing bases -6 to -1. The exchange of Ccm1 for mS47 is coupled to the irreversible removal of precursor rRNA that is accompanied by conformational changes of the mitoribosomal proteins uS5m and mS26. These conformational changes signal completion of 5'-end rRNA processing through protection of the mature 5'-end of the 15S rRNA and stabilization of mS47. The removal of the 5' precursor together with the dissociation of Ccm1 may be catalyzed by the 5'-3' exoribonuclease Pet127. Involved in the specific removal of group I introns in mitochondrial encoded transcripts.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Binds to mitochondrial small subunit 15S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711056.1">
        <property type="nucleotide sequence ID" value="XM_018856067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8T7"/>
    <dbReference type="STRING" id="869754.A0A1A0H8T7"/>
    <dbReference type="GeneID" id="30029043"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033443">
        <property type="entry name" value="PROP1-like_PPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447">
        <property type="entry name" value="OS03G0856100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47447:SF17">
        <property type="entry name" value="OS12G0638900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17177">
        <property type="entry name" value="PPR_long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51375">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PROP1-like PPR" evidence="10">
        <location>
            <begin position="250"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="255"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="repeat" description="PPR" evidence="8">
        <location>
            <begin position="328"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="753" mass="86057" checksum="1EC21EFDC8D90FE2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H956</accession>
    <name>A0A1A0H956_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_16170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710935.1">
        <property type="nucleotide sequence ID" value="XM_018854629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H956"/>
    <dbReference type="STRING" id="869754.A0A1A0H956"/>
    <dbReference type="GeneID" id="30027605"/>
    <dbReference type="OrthoDB" id="446113at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061158">
        <property type="term" value="P:3'-UTR-mediated mRNA destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050825">
        <property type="entry name" value="RBM42_RBP45_47-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640:SF10">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47640">
        <property type="entry name" value="TRNA SELENOCYSTEINE 1-ASSOCIATED PROTEIN 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="49"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="166"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="318"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="449" mass="49056" checksum="538553F1F7C1A079" modified="2016-10-05" version="1" fragment="single">SRTASHQHQHEQELLLRFAVPSPPVATKQLTLAAGHDAAGTGPAAETPRTLWMGDLDPWLDEAAIVALWWHVLGQHVGVKVIRPRSARQGGATSAPPAHAGYCFVEFPLFEAAQQALGLNGQMLPDMAMPSQREFPDNPDNQKKYFRLNWAAGATLLAPLVELPEYSLFVGDLSAATTEAHLLLFFQKLFPNSIRTVRVMTDPVSGKSRCFGFVRFSDEHERQRALVEMNGAWFGGRPLRVANATPRAAPLLRRMLPDPRLAYAVPGPYAGVPDLPQGELMYMPPAQQGASSPMQGYYAAPMMGNEGPHAPYVDSGNTTVFVGGLLAEVSDQTLYTLFKPFGAIVQIKLPPGKNCGFVRYSSREEAQNAINSMEGFVIGGRRVRLGWGRIGPSNKKFQQHQQHQQQMAQMQMQAAMSLAYDPSSVYAAAGYPPMPGVPVGPPGMYHYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9C9</accession>
    <name>A0A1A0H9C9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SART-1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_43268</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNU66/SART1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711014.1">
        <property type="nucleotide sequence ID" value="XM_018857637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9C9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9C9"/>
    <dbReference type="GeneID" id="30030613"/>
    <dbReference type="OrthoDB" id="5583at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000481">
        <property type="term" value="P:maturation of 5S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045347">
        <property type="entry name" value="HIND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005011">
        <property type="entry name" value="SNU66/SART1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152">
        <property type="entry name" value="SQUAMOUS CELL CARCINOMA ANTIGEN RECOGNISED BY CYTOTOXIC T LYMPHOCYTES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14152:SF5">
        <property type="entry name" value="U4_U6.U5 TRI-SNRNP-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19252">
        <property type="entry name" value="HIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03343">
        <property type="entry name" value="SART-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="190"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="588"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="588"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="598"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="611" mass="68028" checksum="71036ADE0825CDCE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9I5</accession>
    <name>A0A1A0H9I5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease subunit Sen54 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_43049</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711060.1">
        <property type="nucleotide sequence ID" value="XM_018857613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9I5"/>
    <dbReference type="STRING" id="869754.A0A1A0H9I5"/>
    <dbReference type="GeneID" id="30030589"/>
    <dbReference type="OrthoDB" id="408683at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024337">
        <property type="entry name" value="tRNA_splic_suSen54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024336">
        <property type="entry name" value="tRNA_splic_suSen54_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21027:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12928">
        <property type="entry name" value="tRNA_int_end_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen54 N-terminal" evidence="4">
        <location>
            <begin position="70"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="350"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="452" mass="52160" checksum="5EC428B1B29597A5" modified="2016-10-05" version="1">MSGGDGEFDLINDDCIEESINDWGLINQNQSSSKINDETLPKRSEKFFDQDGSDAQKLKLEYARDQMYLALLHIRGHHIKQLLVGVWIHSKRKAFIPHAKGSFFKDMGAPHVYKLKKKLQGVWLSEIEAIYLTERGSLVMYAANDQFLEFMENDNLLFNYETLTHLTLAHLQTTAFGLNCELVDKYKVFALLKRLGYSVMEHRALHILLDQCDLPSEPEGKKHLSFFSAIQSFGVSFGTTLRRLYTLLLEKQSHFLTYTLVYKSLALVPTSDFMSFGPPYALDPRYRIIFNVWKPSPHFSKKNPPAPDFQIGILNTSKVAFPDLGSIQGTWAYLDNKRDTECIRKPKETKISKTTDVSNKTKKEMKAQNKAERESRLDPKLRNRNNYLRNRDKLLKQGTTGRSTVFAVVDNGVVNFTTFNETDFRLRYCAKDLNEFETREDHGIVWNERLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9R9</accession>
    <name>A0A1A0H9R9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711384.1">
        <property type="nucleotide sequence ID" value="XM_018855878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9R9"/>
    <dbReference type="GeneID" id="30028854"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="8466" checksum="8B0DD5B8C56B1210" modified="2016-10-05" version="1">MERVTGPLAGLLFFFRPGGQLRPRPPVFRANAACLRLAVFPWAGPPAAQPGPLAPPPPRLLRGSPRANLHPKLAPLIFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA44</accession>
    <name>A0A1A0HA44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_205518</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711269.1">
        <property type="nucleotide sequence ID" value="XM_018854972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA44"/>
    <dbReference type="STRING" id="869754.A0A1A0HA44"/>
    <dbReference type="GeneID" id="30027948"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="353" mass="39858" checksum="BB0B73EA388BC531" modified="2016-10-05" version="1">MQKKIVLQADLAKWTDSASHGALLRFLERLQTAVVGVTNDAPVPGSKTIDSLLQILQQVERLVQAHPVVKDADVSRFGKPEFREFYDDLHARAPAWMAGLCLPETQRYAPEMLAYFAESWGNRTRIDYGSGHELNFVLFLYTLHETGILVEADYTAAIVKVFTQYMAIMRQLQKTYWLEPAGSHGVWGLDDYHFLPFLFGAAQLKTHPHMKPKLIHNKELVESLWRQYMYLECIQFINDIKTVPGVDKASASLRWHSPMLDDISLARLWAKISDGMIKMYRAEVLGKLPIVQHIMFGELFPAPDGLTGHAENVEDDCGHVHPKQGINTWGDCCGIKIPSAVAASQANQRVPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA60</accession>
    <name>A0A1A0HA60_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_205635</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711284.1">
        <property type="nucleotide sequence ID" value="XM_018854978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA60"/>
    <dbReference type="GeneID" id="30027954"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="194" mass="22325" checksum="B10EA470EE4A249E" modified="2016-10-05" version="1">MSRLGLTASIGPHIHISRVRTDQISLVMTAYISRSSYIHFKNSLHSFPEQPTFIPRTTYIQFQTTNIHFKKNLHLLPKYPTFISRQPTFISRKTYTYFQNILHSFPDNLHSFQEKPTLTPKISHIHSRSRPSIRRTHLVSPGTHRTPEPTAPRNSRATQSRPPESPPRACSIPPKKSAVSSPSPVKTKRIRQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA78</accession>
    <name>A0A1A0HA78_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_171439</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711544.1">
        <property type="nucleotide sequence ID" value="XM_018854696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA78"/>
    <dbReference type="STRING" id="869754.A0A1A0HA78"/>
    <dbReference type="GeneID" id="30027672"/>
    <dbReference type="OrthoDB" id="9974421at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004771">
        <property type="term" value="F:sterol ester esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000095">
        <property type="entry name" value="Triglyceride lipase-cholesterol esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11005">
        <property type="entry name" value="LYSOSOMAL ACID LIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="8">
        <location>
            <begin position="151"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="476" mass="54452" checksum="38897FE03F12ECB2" modified="2016-10-05" version="1">MYVPFLGRLSVIEWFLLLVSFWLSWMEYVSNIITKLLPTPVLTLISSTLRLMYKLMGQKINFTTKKLAILNDQDVPYKYVLAADGVDDNRYKLMTGLLNSRNIQDMCLLFGYAVENRIVKTNDGYLLTIQRLTGRKDEAGNFRQLPRNGKVVYMHHGLLMCSEIWVTMLDRENNLPFVLYDLGYDVWFGNNRGNKYSQKHMTRPLNSEAFWNFSIDEFALFDIPDSINFILEETGKQHLTYIGFSQGTAQAFASVSVNPELTKKIDKIIAISPATTPHGLYSKFLDILLKSSPKMLFLLFSRKALLPSVVFWGRIMYPPFFNTSIDLSNYLLFYWKAENIDKIQKLCSYAHLYSTTSVKTVVHWFQIMQLKNFQMYHDATSGMNSLNPISYPLSNISIPIHLIYGDSDSLVDIDVMKSQLPAETTHTHKIANHEHLDNLWGRDVSETVFPLVLAALGETPNGTIAKLAGLKKTVNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA95</accession>
    <name>A0A1A0HA95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5-hydroxyisourate hydrolase</fullName>
            <shortName evidence="7">HIU hydrolase</shortName>
            <shortName evidence="7">HIUHase</shortName>
            <ecNumber evidence="7">3.5.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_11529</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711435.1">
        <property type="nucleotide sequence ID" value="XM_018854014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA95"/>
    <dbReference type="STRING" id="869754.A0A1A0HA95"/>
    <dbReference type="GeneID" id="30026990"/>
    <dbReference type="OrthoDB" id="10265230at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395:SF7">
        <property type="entry name" value="5-HYDROXYISOURATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="URICASE AND TRANSTHYRETIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16681" checksum="D075B5E47C91C81B" modified="2016-10-05" version="1">MSVDPITCHILDTTLGKPAQNVTCSIFYLSPLITNKNDEAAYDLDPAVQPFAMSRTDNDGRIKKWVVNPGNGDGVNSKVGVENDEWSVLKPGVYKIKFLTGKYFHDIDASSRTFFPFVDITFQIDSPPDHHYHVPLLLSNHSYSTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG4</accession>
    <name>A0A1A0HAG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pyrophosphate phospho-hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31534</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711377.1">
        <property type="nucleotide sequence ID" value="XM_018855875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAG4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAG4"/>
    <dbReference type="GeneID" id="30028851"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000007">
        <property type="entry name" value="Inorganic pyrophosphatase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="333" mass="36801" checksum="F32FA6A398F19779" modified="2016-10-05" version="1">MAKPTPPVPLQARTRLAATLRHLSSAAPKSKGAQPALESPNAPKAHYVSVGVGSKYTKEYKNYCVEAPAKVMSYFHDVPLGLDLERKTATVVVEIPRWLNAKFEINIGEPGNPITQDVKKGRVRFVKNLFPYKGFPFNYGAFPQTWEDPTVMHDVAGLPGDGDPLDVCEIGARVLPTGTVKTVRILGSLALVDDGELDWKVIVVDEQDPLAGELRDIGDVLEKCPGLLEATREWFRNYKLPDGKLANKFAFDETYRSQQETMDLIMQCHGAWRRLVQGRSAAEGFAIENTTVENTPGHVARFAFSDAVLGSDVESDAEIPPDVSRSHFFKHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAL4</accession>
    <name>A0A1A0HAL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_229507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711428.1">
        <property type="nucleotide sequence ID" value="XM_018855228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAL4"/>
    <dbReference type="GeneID" id="30028204"/>
    <dbReference type="OrthoDB" id="4087050at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="224" mass="25233" checksum="29F1A1F1E5EC7745" modified="2016-10-05" version="1">MLAKKKANFIAYILFLSYAFAITVLGIRDVPERIVHTRTNPTTAKNDNVGSTASASSLHLMGVVPETRVKLKTDYIKEFLAFLSLNSFRTLAEPEKAPLKTKFTNLERILSDDTDLRTWTRFIVEDDRDEDFTPPMESSGAESIDLHIDDFIDYLVEHRGFSANDLQFLHDNNFDSDYDGIDDVLTTIKSKSAANDDSMSFLPSSGSVVPIKYLWVLPLIICLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAX0</accession>
    <name>A0A1A0HAX0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Alpha/beta-hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_78122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711537.1">
        <property type="nucleotide sequence ID" value="XM_018858825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAX0"/>
    <dbReference type="GeneID" id="30031801"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003386">
        <property type="entry name" value="LACT/PDAT_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11440">
        <property type="entry name" value="LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02450">
        <property type="entry name" value="LCAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="635" mass="70943" checksum="883BF0AEA688B681" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB23</accession>
    <name>A0A1A0HB23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable electron transfer flavoprotein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_11771</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 10">Binds 1 FAD per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711721.1">
        <property type="nucleotide sequence ID" value="XM_018854080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB23"/>
    <dbReference type="STRING" id="869754.A0A1A0HB23"/>
    <dbReference type="GeneID" id="30027056"/>
    <dbReference type="OrthoDB" id="1715808at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000041">
        <property type="entry name" value="Electron transfer flavoprotein alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000001">
        <property type="entry name" value="Electron transfer flavoprotein, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153:SF1">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 9 10" id="KW-0274">FAD</keyword>
    <keyword evidence="5 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Electron transfer flavoprotein alpha/beta-subunit N-terminal" evidence="11">
        <location>
            <begin position="20"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="248"/>
            <end position="249"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="262"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="279"/>
            <end position="286"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000089-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="330" mass="35097" checksum="B9417D460D0AC352" modified="2016-10-05" version="1">MLRSLIYKRSFSTTKISRQTLAFIEVSNGSITSLSLSAITAAQNLGKPITAILAGTQAEKVASSTSKIDGISKVLSAGNGQYDHYLAEELVPLLKEVIETRKFTHMITPASAVGKSFLPRLGALLDVQPISDVVEVLSEDTFVRPIYAGNALATVKSNDLTKLVSIRASAFQPAKFNTESVSIEEIDSLEKSDNRTKFLSEQLIESDRPELGSATKVVAGGRGLKNRETFESLIEPLASKLNAAIGASRAAVDSGFCDNSLQVGQTGKIVAPDLYIAVGVSGAIQHLAGMKDSKTIVAINKDPEAPIFNIADIGLVSDLHEAVPELSNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB98</accession>
    <name>A0A1A0HB98_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleolar protein 56</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_11830</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for 60S ribosomal subunit synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711796.1">
        <property type="nucleotide sequence ID" value="XM_018854108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB98"/>
    <dbReference type="STRING" id="869754.A0A1A0HB98"/>
    <dbReference type="GeneID" id="30027084"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000001">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000002">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="9">
        <location>
            <begin position="298"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="441"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="496" mass="55434" checksum="972EB8BA7EF506ED" modified="2016-10-05" version="1">MAGIDYLLFEEATGFAIFKVLIQQDDIAVRLKEVQEASLDLGKFSKMIELVSFAPFKGAAQALENANDISEGLVSDYLKSVLELNLPKPSKKERITLGVSDKNLGPSIKEHFPYVDCLSNEIAQDFLRGIRVHGPKLLKDLQEGDIERAQLGLGHAFSRAKVKFSVQKNDNHIIQAIALLDQLDKDINTFSMRVKEWYGWHFPELAKIASDNYVYAKLALYIKDKANLTDENVHDIAALFNDDLSIAQRIIDNAKISMGQDISELDMLNVSTFAERVVSISEYRARLYQYLTDKMHTVAPNLSTLIGEVVGARLISHAGSLTNLSKQAASTVQILGAEKALFRALKTKGNTPKYGLIYHSSFIGKAATKNKGRISRYLANKCSIASRIDNYSDEPTTAFGQILRKQVEDRLNFYDTGAAPMKNADAIKQALALGGADAMDVDAEEEEAVEEPKKEKKEKKEKKDKKDKKDKKDKKEKKRKADDDEAPKKKKKKSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBF6</accession>
    <name>A0A1A0HBF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Concanavalin A-like lectin/glucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711979.1">
        <property type="nucleotide sequence ID" value="XM_018855959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBF6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBF6"/>
    <dbReference type="GeneID" id="30028935"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07308">
        <property type="entry name" value="lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF45">
        <property type="entry name" value="RE50040P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51328">
        <property type="entry name" value="L_LECTIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="7">
        <location>
            <begin position="53"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="395" mass="45333" checksum="11AD3ABF81D37886" modified="2016-10-05" version="1">MSALRTRVLMALVSLASLVAVYWFVFMGKPEEFSPEEINDMFQNKKSEVSVKRVELPEHAISSPYLDQHTLKLANWDLEGDVVVQNGQHISLTSDHKHQASRMFLKAPVEAESFEMELTFHIHSEEKLLPDGMAVWFTADPLPLGDVFGVQNNFKGLGIFMDTFQNGRRGGFPLVNAQYSSSSLFYDKHTDGMDSMIASCLPKPIVNPALGKTRMRIVHTRNGYLSVDFNHDPDQSSEWKNCFSVMDVVLPEKFYIGISAETGELYENVDIIESKIYALYLPDFDHYIESIDELEHIMQQQAPVGNQGVSKERRRKSISRLRNAERRIKENERAHRLAKYGDPEATFVHRWVARIKTLFKYSLYMILFLLVLWIARIAIKSRKGRRSRKAVGLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBF7</accession>
    <name>A0A1A0HBF7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711830.1">
        <property type="nucleotide sequence ID" value="XM_018854114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBF7"/>
    <dbReference type="GeneID" id="30027090"/>
    <dbReference type="OrthoDB" id="4024574at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="56609" checksum="FADD902726A6CC14" modified="2016-10-05" version="1">MSLATRPAPRILQPSTGRKITRKAIATAHRAMKLLVHAIFMFCFVPLALAGICSSNTVVPFSGYKVTRRHKIRLRPLQSDSYLVLTKCHRGLPVFSRTVATPSAISEFSIPFTSADTELDFLDRWGRRGKKEQRNSDDRESPGDLSESEHRRGSNMRPGRGLAFRAWLSSFGWMHAHEPAQGYINGGIETSSIRSGTPGARTRRARGQQERIPRSRSLRTKTVGASILNSPLFETSTVRNQAQARVADTESLRMTEIKTRGLKLTGPARRWRNQPFPAPAPCLKLARRKRYARLDVVIFIPATYVGVLFTCNPQDSTHDEEQEWRWDAQAIEKRLPMATEARRYPRHRQGASLENNQQTSKLLQRASKSERQESSHRDISSPAHKFAMAIAAKDKFIESQPGTAAYETEFSGAPVCPLVADDYSAHWLSVQLHPPALVYHIPYLRSPSIWDPRFLSSETELPEWSEDVDINEAYIYSEADLAGINENLMKSTRPKRPRSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBL6</accession>
    <name>A0A1A0HBL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_41453</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SDA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712039.1">
        <property type="nucleotide sequence ID" value="XM_018857402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBL6"/>
    <dbReference type="GeneID" id="30030378"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027312">
        <property type="entry name" value="Sda1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048292">
        <property type="entry name" value="SDA1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007949">
        <property type="entry name" value="SDA1_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012977">
        <property type="entry name" value="SDA1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730">
        <property type="entry name" value="HSDA/SDA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12730:SF0">
        <property type="entry name" value="PROTEIN SDA1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21638">
        <property type="entry name" value="SDA1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05285">
        <property type="entry name" value="SDA1_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08158">
        <property type="entry name" value="SDA1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SDA1 N-terminal" evidence="9">
        <location>
            <begin position="64"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 middle" evidence="8">
        <location>
            <begin position="543"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="SDA1 C-terminal" evidence="10">
        <location>
            <begin position="666"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="552"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="643"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="554"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="643"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="715" mass="80489" checksum="3824A2625CCAFCA9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBN0</accession>
    <name>A0A1A0HBN0_9ASCO</name>
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        <recommendedName>
            <fullName evidence="6">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_179531</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018854756.1"/>
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    <dbReference type="OrthoDB" id="1893551at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="215"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="277"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="330"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
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        </location>
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            <end position="1307"/>
        </location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1448"/>
            <end position="1484"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="13"/>
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    </feature>
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        <location>
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        </location>
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            <end position="1464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="1472"/>
            <end position="1484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OBA21293.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1484" mass="163568" checksum="8D2CB15AA463C7AE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBP1</accession>
    <name>A0A1A0HBP1_9ASCO</name>
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        <recommendedName>
            <fullName evidence="7">SPX domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="8" type="ORF">METBIDRAFT_41159</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712064.1">
        <property type="nucleotide sequence ID" value="XM_018857364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBP1"/>
    <dbReference type="GeneID" id="30030340"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14474">
        <property type="entry name" value="SPX_YDR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="745"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="784"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="821"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="7">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="843" mass="95381" checksum="82CCABFF4DC7B134" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBR0</accession>
    <name>A0A1A0HBR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA decay factor PAT1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_69957</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">P-body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711930.1">
        <property type="nucleotide sequence ID" value="XM_018858384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBR0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBR0"/>
    <dbReference type="GeneID" id="30031360"/>
    <dbReference type="OrthoDB" id="74835at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039900">
        <property type="entry name" value="Pat1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019167">
        <property type="entry name" value="PAT1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551:SF0">
        <property type="entry name" value="PROTEIN ASSOCIATED WITH TOPO II RELATED-1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21551">
        <property type="entry name" value="TOPOISOMERASE II-ASSOCIATED PROTEIN PAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09770">
        <property type="entry name" value="PAT1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="92"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA decay factor PAT1" evidence="8">
        <location>
            <begin position="527"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="210"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="421"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="457"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="104"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="221"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="795" mass="86605" checksum="6804221707E99EB0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS7</accession>
    <name>A0A1A0HBS7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Regulatory protein SIR2 homolog 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_42409</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-glutaryl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-glutaryl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11875"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87828"/>
            <dbReference type="ChEBI" id="CHEBI:87829"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-malonyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-malonyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11878"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87831"/>
            <dbReference type="ChEBI" id="CHEBI:87833"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N(6)-succinyl-L-lysyl-[protein] + NAD(+) + H2O = 2''-O-succinyl-ADP-D-ribose + nicotinamide + L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-69 and Arg-72) that bind to malonylated and succinylated substrates and define the specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711853.1">
        <property type="nucleotide sequence ID" value="XM_018857540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS7"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS7"/>
    <dbReference type="GeneID" id="30030516"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061697">
        <property type="term" value="F:protein-glutaryllysine deglutarylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="146"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 5">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="104"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="243"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="304" mass="33142" checksum="E7493A5750EB38BF" modified="2016-10-05" version="1">MANPAHLAEFQECLKSSRRIIALVGAGLLASSGLPTFRGSQGLWKNFNLIDLATPDAFYIDPGLVWQFYLWRRHQALQAQPNAGHLALANLSKDPSKEFVTITQNVDGLSGRAGHERENLFEIHGSLFDLRCTSFTCTYVERGNEEDPLTPALDTFDEYESPPARKSDQVGGPSKDLTNVPGKSPQFTPTKELAVSELPLCPACKSLMRPGVVWFGELLPLRVLGAIDDFLETGPVDLILVIGTSGTVYPANGYVDLVRSRGGAVAVFNTDIEPEVLKGNVARTWGFEGDAAEWLPRALACLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBV1</accession>
    <name>A0A1A0HBV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyoxylate reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41472</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711970.1">
        <property type="nucleotide sequence ID" value="XM_018857407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBV1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBV1"/>
    <dbReference type="GeneID" id="30030383"/>
    <dbReference type="OrthoDB" id="9991913at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047964">
        <property type="term" value="F:glyoxylate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009436">
        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12168">
        <property type="entry name" value="Mand_dh_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF257">
        <property type="entry name" value="GLYOXYLATE REDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="44"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="122"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="37947" checksum="4B610E8B5B2C079B" modified="2016-10-05" version="1">MAPAKPKVLRLGSWALSGPKWHDLAAIADVVDCQSQSRQQFFDDLKTKYADVSCITRTFFSVSQTGRFDAELALHLPESVRSISHCGAGYDQIDVAPFTARNIQVSNVTSPVEAPTALTAVYLTLAALRNFQQGHDLLTQGWWPQAKCAGAHVGRDPEGLTVGIVGMGGIGRAIRDRLAPFGFARFVYYNRTRLDADAEKGAQYMPLDALVATADVVMLSVPLNAGTRHLVDAAVLSRMKKGVVIINTARGAVIDEAALTDALLRGHVGAFGADVFEHEPQVSPRLYGLPNVVSLPHMGTHSQQAYRNMENFVVENVRTYLATGTMLTNVPEQAGVDFQHDILVPPGLGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCC8</accession>
    <name>A0A1A0HCC8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_15140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712042.1">
        <property type="nucleotide sequence ID" value="XM_018854570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCC8"/>
    <dbReference type="GeneID" id="30027546"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="142" mass="15749" checksum="4C450B88C92B23D1" modified="2016-10-05" version="1" fragment="single">NDPVPGNLFWFREHFIIPVDLSEPRDMLQSERTLLGFVRFALTLSFTAIGLILGFHLKSRGAKNGSDVGPRWSLFNQAVPFFLAFLALATLATSIANYFQTVKRYSRHRIQNSGPKSALLVVCVTAVVVTLAGLNISLTVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCE6</accession>
    <name>A0A1A0HCE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U2 small nuclear ribonucleoprotein A'</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_38784</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712280.1">
        <property type="nucleotide sequence ID" value="XM_018857047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCE6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCE6"/>
    <dbReference type="GeneID" id="30030023"/>
    <dbReference type="OrthoDB" id="433501at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="238" mass="25869" checksum="70645275CA06FD9A" modified="2016-10-05" version="1">MLGLRLTPGAVQSALKVAAPQGLLTLVLRSRGLLYIENLEYTQDAFSAIDLTDNEITELSGLPELRKLEVLLLANNNIASVGRVAASALTSLLLMNNSLSSFAEVAKLRGLRRLEHLVLLGNRITEEHHYRGFVVWLFPTLKVLDCQKVKPAERRAAAELFGPAFDSRLPAADALLSGSQAAAPVPKDTRLMETTMRKLLAEEKALLAAELEGASSMAEIERISRALKRGYVGTAENP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD18</accession>
    <name>A0A1A0HD18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pkinase-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_40220</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712475.1">
        <property type="nucleotide sequence ID" value="XM_018857247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD18"/>
    <dbReference type="STRING" id="869754.A0A1A0HD18"/>
    <dbReference type="GeneID" id="30030223"/>
    <dbReference type="OrthoDB" id="10252354at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004708">
        <property type="term" value="F:MAP kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050850">
        <property type="term" value="P:positive regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000263">
        <property type="entry name" value="MAP kinase skh1/pek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050915">
        <property type="entry name" value="MAP_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448">
        <property type="entry name" value="DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE DSOR1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47448:SF5">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINAE MKK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="82"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="372" mass="41395" checksum="31572D199BA10F9F" modified="2016-10-05" version="1">MALEVSVLNAPVSLPYIPTSEPSPGRLRRKPVPLDFTKIDGLHSHVPEQGKPSLIVSSPGPAAQLDHVTPEEWNFLANSSQIEELSKLGEGNGGSVSKCVLKCGSPVFALKLINTDPNPDIQKQILRELQYNRLCHSPNIVKYYGTFMVEKQLMIGIAMEFMGGGTLDAIYKHVLELDPTNRVSEKVLGKVADSVLKGLDYLHLQKIIHRDIKPSNILFDTQGNVKICDFGVSGDVVNSLATTFVGTQYYMAPERIMGQPYTVSCDVWLLGVTLLEVAQGRFPFLAQNSNPLGPIELLLLILECEPKLEDNPAEQIHWLDSFRNFLGYCLKKVPEDRPGPRQILQHPWCIGQLRFNVNMAKFVRKVWGIQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDA1</accession>
    <name>A0A1A0HDA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Altered inheritance of mitochondria protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">AIM41</name>
        <name evidence="4" type="ORF">METBIDRAFT_39185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the AIM41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712446.1">
        <property type="nucleotide sequence ID" value="XM_018857108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDA1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDA1"/>
    <dbReference type="GeneID" id="30030084"/>
    <dbReference type="OrthoDB" id="538640at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016884">
        <property type="term" value="F:carbon-nitrogen ligase activity, with glutamine as amido-N-donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.10">
        <property type="entry name" value="Uncharacterised protein YqeY/AIM41 PF09424, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019004">
        <property type="entry name" value="YqeY/Aim41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042184">
        <property type="entry name" value="YqeY/Aim41_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28055:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 41, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09424">
        <property type="entry name" value="YqeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="171" mass="19843" checksum="636CB8572D33431B" modified="2016-10-05" version="1">MFASVRRLQASSAYTQLMALMKQDLKQAMLAKNKIEKNTIRSVLSSIKNSEIDGGKQTEFDLYRLFNKMIKQRDESHQLYVSQQRLDLAEVEALESQIIRRYLASLPVASEQEIAAKTEQWLVELREKNGTDLQMKDVMKQVTDEFAQNWNTFPAAIRSLVPLLYKRLWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDE8</accession>
    <name>A0A1A0HDE8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_39195</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712537.1">
        <property type="nucleotide sequence ID" value="XM_018857109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDE8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDE8"/>
    <dbReference type="GeneID" id="30030085"/>
    <dbReference type="OrthoDB" id="2155291at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF17">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN RGD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50186">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="5">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="3">
        <location>
            <begin position="214"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="4">
        <location>
            <begin position="475"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="704"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="704"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="800"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="852"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="871" mass="98895" checksum="C31600B6CC6805F5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDF6</accession>
    <name>A0A1A0HDF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ornithine decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_77596</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-ornithine + H(+) = putrescine + CO2</text>
            <dbReference type="Rhea" id="RHEA:22964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="4.1.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Only the dimer is catalytically active, as the active sites are constructed of residues from both monomers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Orn/Lys/Arg decarboxylase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.17" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712610.1">
        <property type="nucleotide sequence ID" value="XM_018858729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDF6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDF6"/>
    <dbReference type="GeneID" id="30031705"/>
    <dbReference type="OrthoDB" id="5034579at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004586">
        <property type="term" value="F:ornithine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000005">
        <property type="entry name" value="Ornithine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF6">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="11">
        <location>
            <begin position="83"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="397"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="450" mass="49709" checksum="E41CDA4CE7ED7DDD" modified="2016-10-05" version="1">MAPSVEVLNNAESYLIAEDAIKIPANPAGNVPEDAYYVEKQSSSGGSEEWPGLVKRALLAQVDQIDHDVCDAGDEDSFFVADLGQVAKAYRLWEQKLPQVHAHYAIKCNTDEEVVKLLGSMGANFDCASKNEIDTVLRLGFDSSRIVYANPCKTNSFIRHAKNSNVNLTTVDNKQELYKLKKFHPDCKVLIRIATDDESAQCRLSTKFGCTLETALEELLPECLHLGIDAVGVAFHVGSGAKDFDSIFQATKQARMIFDKAQLLGAPMTLLDIGGGFERETFEESSRMVRSSLKKFFPSEYVERNGVRIIAEPGRFMVANAFTLATHIIARRDLDRGADGMEAMLYINDGVYGNLNCILFDHQHPVARVLKSEGRVYNDFGEKHAEEYTFLIWGPTCDGLDCVAPRAVLPANVHVGDWLYFPNLGAYTSAATTSFNGFSGRSVVKYVSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDN6</accession>
    <name>A0A1A0HDN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712586.1">
        <property type="nucleotide sequence ID" value="XM_018855760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDN6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDN6"/>
    <dbReference type="GeneID" id="30028736"/>
    <dbReference type="OrthoDB" id="2135762at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013950">
        <property type="entry name" value="Mis14/Nsl1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08641">
        <property type="entry name" value="Mis14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="239" mass="27649" checksum="C9F5855A7C1278AF" modified="2016-10-05" version="1">MGANDHYTKILLSKSELAHVYRQVFYASQEKFKKLLPGCAPHDEFLMQVQNMMSANLETTFEKAKYALIVDGEDLADNDVHLNKLLTIEEREEVVPFDTELNQQLRDLIKQVENETTEVTQLRRELPQEARAAYEKLVLSIDQQVTDFIKEITENAETDENFGSLDSGMKELMSAMESSQVPIQNSDTIRARILESLQNLYSLKKELPQLRSELQSCDDTTAFMMNVYEKLKVERRLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDS6</accession>
    <name>A0A1A0HDS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Nitrogen permease regulator 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Required for meiotic nuclear division protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_38680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Mediates inactivation of the TORC1 complex in response to amino acid starvation. Required for meiotic nuclear division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Vacuole membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the NPR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712543.1">
        <property type="nucleotide sequence ID" value="XM_018857029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDS6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDS6"/>
    <dbReference type="GeneID" id="30030005"/>
    <dbReference type="OrthoDB" id="18648at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990130">
        <property type="term" value="C:GATOR1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056603">
        <property type="entry name" value="HTH_NPRL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005365">
        <property type="entry name" value="Npr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153">
        <property type="entry name" value="CGTHBA PROTEIN -14 GENE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13153:SF5">
        <property type="entry name" value="GATOR COMPLEX PROTEIN NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24064">
        <property type="entry name" value="HTH_NPRL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03666">
        <property type="entry name" value="NPR3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="GATOR1 complex protein NPRL3 C-terminal HTH" evidence="6">
        <location>
            <begin position="692"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="112"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="761" mass="85927" checksum="A26DF48D8A559A6E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDV5</accession>
    <name>A0A1A0HDV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PLP-dependent transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712770.1">
        <property type="nucleotide sequence ID" value="XM_018855806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDV5"/>
    <dbReference type="GeneID" id="30028782"/>
    <dbReference type="OrthoDB" id="7042322at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42858:SF1">
        <property type="entry name" value="LD15494P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="1">
        <location>
            <begin position="49"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="439" mass="48988" checksum="4FB9E9C2BECAE132" modified="2016-10-05" version="1">MTRPINFFKGHPTLTLLPNQELADAYRRVLVEQNFSHSDNDPSNRYPLEYGTDPGNLSTRETILQWSNSRYGRKTDDPDVVNLTAGSSCGAANILLACTRSNITKHVFLVSPTYFLINYAFIDAGFEGQMSAVMETAGEEYDIDIAGLEQKLQELDRKHGLEAVSAKEINVIHDPTQRGPRKFYRYAMYLVPTFSNPGGISYSTKTRSKLLEIARKHDVLLISDDVYDLLDFGLETPKLPKINHIDQDTLPDGWVYGNSVSNSSFSKLIAPGLRTGWQETATPCLAQQLATTGSNKSGGTPAQLNICVVQNMIQTGDLDRCISRLNLAFRARAKALVSALQRHLPAKYLKIGGGQGGYFVWCTIDAEDVDVGKTLEILRDEHQVLIAEGHHFEVEGDSKGWGKKCARLCVAYLDEEEIEEGISKWGEIMQREYPGLYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ2</accession>
    <name>A0A1A0HDZ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_15406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712613.1">
        <property type="nucleotide sequence ID" value="XM_018854597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDZ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDZ2"/>
    <dbReference type="GeneID" id="30027573"/>
    <dbReference type="OrthoDB" id="432953at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000138">
        <property type="term" value="C:Golgi trans cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026705">
        <property type="entry name" value="Hid-1/Ecm30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21575:SF12">
        <property type="entry name" value="PROTEIN HID1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12722">
        <property type="entry name" value="Hid1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="649"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="987"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="987" mass="108329" checksum="E22EC57AE8E021ED" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB2</accession>
    <name>A0A1A0HEB2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Plasma membrane iron permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712945.1">
        <property type="nucleotide sequence ID" value="XM_018858778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEB2"/>
    <dbReference type="GeneID" id="30031754"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="294"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="43116" checksum="83D0F3CD7E7DCE61" modified="2016-10-05" version="1">MVDVFNVEIFFVVFRESLEAVVVVSVLLAFLKQGLGGATEDPVVYKRLRKQVWLGSGLGLLICLCIGCAFIAVFYTLKDDVWSESEDLWEGIFCLIATIMITFMGLAMLRVNKMKEKWRVKLAQALVKTPEDSKKKWLGFGHYAKKYAMFILPFITVLREGLEAVVFVGGVGLSSSATSFPIPVICGLLAGLLVGVIMYYTGANFSLQIFLIVSTCVLYLISAGLFSRAIWFFETYVFNQLTGGDASENGSGPGTYNIKKSVWHVNCCNPELDNGWDVFNALLGWQNSATYGSVISYNIYWLFIIVTVFLMGFEEKYGHLPLLKNVTLRSLNPMYHIKGKKKNELSKAERDALFEKVKNQRIDDEERSNESLMNKAEKTSVSGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB5</accession>
    <name>A0A1A0HEB5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712826.1">
        <property type="nucleotide sequence ID" value="XM_018855818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEB5"/>
    <dbReference type="GeneID" id="30028794"/>
    <dbReference type="OrthoDB" id="94039at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050228">
        <property type="entry name" value="Carboxylesterase_BioH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194">
        <property type="entry name" value="HYDROLASE ALPHA/BETA FOLD FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43194:SF2">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN LPX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="50"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="369" mass="41323" checksum="8E34716AAE98D382" modified="2016-10-05" version="1">MVHLEKKQTQAYWPRGPGSYVSMTDKLDIVYLKYTSADPVPQGCTTVNFIFAHGTGMNKSLWKRHIEELYGKNKSCTGWKVGCVLSVDAVGHGDSALINKEKISWANDWRDGAKDLIETTGDFLPSAYTRNIVVGHSMGGFHATYASFLEPTLFDACVAIEPVIFIDQGANPLAVARMKKIGLVLRDQFASRADADAFFQKGSFYRVMDPRVLADFTSDELHWENGQLRTKATKDAQLAAYLSAVYSVRFGQNILLLLEVPYLLITGEQAAWNSPKSDRHIEKSVSSHLLETVKLRGDHLVHALNVGDTVDAIYSFAGRRAEFVASHRNEFPEVKLENSAGARVKAMFKHMWKGDMDKAFRWDRPEAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEC7</accession>
    <name>A0A1A0HEC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_86941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712836.1">
        <property type="nucleotide sequence ID" value="XM_018859270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEC7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEC7"/>
    <dbReference type="GeneID" id="30032245"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000260">
        <property type="entry name" value="Forkhead transcription factor (Sep1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="148"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="148"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="262"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="465"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="124"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="391"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="466"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="499" mass="54348" checksum="181202544AD64AEF" modified="2016-10-05" version="1">MSLLAHRSQVLVSHPRDLSDTPLTRVSGRISTPPHSIAGKRTHADLATPVRACDAPAKAVLLSPAFSSPQPPEQPVFDKADMMHAASRSFSDAISEPFSESLSETFSDGHVAEPVPVSQDASGHKRKKPKKVRREPRDACFSLASSEKPPYSYATLIGMAILSHPAKQLTLSQIYLWISETFKYYRREDVGWQNLIRHNLSLNKAFVKGEKSKDGKGHFWCIESNSEDLFLKAKNNKKSLYHEVMDQLALVNRQPHALIPLSPPSLADDDTQNPRSIGATHDDVDGDESNRFPSATMPCLSTPGRAEGASDLSEHSLLVGRSLGFTLSFSCSLNFELLPLPTLRTGPLLEPLSPGGNALLAQNLGHLLVHLPNISVSKSGAHLTQLQPPMTGTSTPRAAGTARTTPKSNMKTPLRLLRTPMSGSMIHKLGYSPSYLEEFNHSPFAAGRTMLNSYDDDDMLMRAFDSPAASKSSKPSLLGELTKATSEDVNTTDASQSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEJ3</accession>
    <name>A0A1A0HEJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Polyadenylate-binding protein</fullName>
            <shortName evidence="14">PABP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_31305</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the polyadenylate-binding protein type-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712901.1">
        <property type="nucleotide sequence ID" value="XM_018855827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEJ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEJ3"/>
    <dbReference type="GeneID" id="30028803"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008428">
        <property type="term" value="F:ribonuclease inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060211">
        <property type="term" value="P:regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12378">
        <property type="entry name" value="RRM1_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12380">
        <property type="entry name" value="RRM3_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12381">
        <property type="entry name" value="RRM4_I_PABPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1900.10:FF:000008">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000003">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000211">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000520">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000648">
        <property type="entry name" value="Polyadenylate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.10">
        <property type="entry name" value="c-terminal domain of poly(a) binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036053">
        <property type="entry name" value="PABP-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006515">
        <property type="entry name" value="PABP_1234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002004">
        <property type="entry name" value="PABP_HYD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034364">
        <property type="entry name" value="PABP_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01628">
        <property type="entry name" value="PABP-1234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00658">
        <property type="entry name" value="MLLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00517">
        <property type="entry name" value="PolyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63570">
        <property type="entry name" value="PABC (PABP) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51309">
        <property type="entry name" value="PABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10 13 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="49"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="137"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="230"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="15">
        <location>
            <begin position="333"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="PABC" evidence="16">
        <location>
            <begin position="529"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="616" mass="68795" checksum="C816C9E7A4D56E14" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEL6</accession>
    <name>A0A1A0HEL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">protein disulfide-isomerase</fullName>
            <ecNumber evidence="4">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_40085</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712911.1">
        <property type="nucleotide sequence ID" value="XM_018857228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEL6"/>
    <dbReference type="GeneID" id="30030204"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000107">
        <property type="entry name" value="Protein disulfide-isomerase 5-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF132">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13848">
        <property type="entry name" value="Thioredoxin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291920" description="protein disulfide-isomerase" evidence="13">
        <location>
            <begin position="24"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="9"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="380"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="512"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="521"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="11">
        <location>
            <begin position="426"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605792-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="543" mass="60447" checksum="2E3E9CD6181A0CB3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HER0</accession>
    <name>A0A1A0HER0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_39246</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2 11"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712956.1">
        <property type="nucleotide sequence ID" value="XM_018857118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HER0"/>
    <dbReference type="STRING" id="869754.A0A1A0HER0"/>
    <dbReference type="GeneID" id="30030094"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-79 specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="13">
        <location>
            <begin position="740"/>
            <end position="1061"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="398"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="490"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="398"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="491"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1061" mass="122489" checksum="D985B4E906AFE2D7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEV4</accession>
    <name>A0A1A0HEV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713001.1">
        <property type="nucleotide sequence ID" value="XM_018853962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEV4"/>
    <dbReference type="STRING" id="869754.A0A1A0HEV4"/>
    <dbReference type="GeneID" id="30026938"/>
    <dbReference type="OrthoDB" id="2147978at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="250" mass="29195" checksum="2ECE73BF6AA4558D" modified="2016-10-05" version="1">MPRFTFSVPFGSLFKLKPGKFNYALEQDIRAKLDTLPSVSKIYSNPDGTPRVPTDSEFKTIAELQNLFYRRNHSQWNFILPGIPREQLELFKDNSQDLQNFQFVGKDAGKVVDKIPYKDETTGELKWRVVRETEKAEGWELPYFYIILPVFFYVVYSKYTAQMKKRAEEGPEWARKELRLRAMEEYFQGDTEKAKEFLSNEGKSERELSDRDDLVVARILAGDYDKLASLKKKMPADLVPQPERNEKIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEX8</accession>
    <name>A0A1A0HEX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mediator of RNA polymerase II transcription subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mediator complex subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_38989</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the Mediator complex subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713026.1">
        <property type="nucleotide sequence ID" value="XM_018857077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEX8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEX8"/>
    <dbReference type="GeneID" id="30030053"/>
    <dbReference type="OrthoDB" id="5310959at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019680">
        <property type="entry name" value="Mediator_Med1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35041:SF4">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10744">
        <property type="entry name" value="Med1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0010">Activator</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Mediator complex subunit Med1" evidence="8">
        <location>
            <begin position="8"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="428" mass="47994" checksum="00E1711B5911DF1C" modified="2016-10-05" version="1" fragment="single">MPYESALQDSLRRLYSESSEFRISIEIIVKLAKSLSLDTFVDTEEFPGVTRLSIAGSLLLLEIDFEDDHTVSKVSLSLGNHPLETLAEENSSAKISGNIVSETATSVSSKNGAKTVVLSFLPDLRASFLRTLQTQLGLGQSSGSVAEEILFASLEGPKLGSFPRNLEYLADLDRVSPPEGDLIVYIENLAMYMSAIHHQECILNPEDWQIADGLTNSVGKVILNDKDQRHVGVFLQFWQDCRVLNHYLIQDQRPQMGRKYSALLAIEESKSPAVDYVLDAKSKPWHILTSSGEKLPYFFGGETEFAHLHNHQSVTANSNWKLVLRFAEPIFFPETLLQYLGITDYECAKPLELNNMWNEIAETGELRFKNTALEYVFAFDEFAPHVNLLAVSLGNLHILAELLPALRNQITFMKIFESVALDKNSEYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEY3</accession>
    <name>A0A1A0HEY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DOCKER domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_40289</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DOCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713031.1">
        <property type="nucleotide sequence ID" value="XM_018857254.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HEY3"/>
    <dbReference type="GeneID" id="30030230"/>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11684">
        <property type="entry name" value="DHR2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057500">
        <property type="entry name" value="C2_DCK1_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF10">
        <property type="entry name" value="MYOBLAST CITY, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25338">
        <property type="entry name" value="C2_DCK_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DOCKER" evidence="3">
        <location>
            <begin position="1253"/>
            <end position="1662"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1710"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1723"/>
            <end position="1733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1748" mass="193324" checksum="EE10D21482BC105C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFA1</accession>
    <name>A0A1A0HFA1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_31444</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713059.1">
        <property type="nucleotide sequence ID" value="XM_018855852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFA1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFA1"/>
    <dbReference type="GeneID" id="30028828"/>
    <dbReference type="OrthoDB" id="756370at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045227">
        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="139"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="190"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="471" mass="48891" checksum="2F736658ECCCC96E" modified="2016-10-05" version="1">MDEVVLYVTAGDPADKHAQESVAAVASVHKLQQHATFRHAQCPVNGAAVSGLGAGSRLFVAGTDRALISTYVWGKEAPDQRLAVPELMACLALAPQPAPARSADAAKTTHSVPWLLAAGLASGKLYVWEVALGDLVCVKDAHYQRVASLAFSPCGSFLVSGGHDTRVNVWRTADLVAPHTALRCKPHALFSDHALAVTGVAFVAAPLGAGSLVASASRDGTLRIYDVAARCLQTTLVFLAAVECFARDPAGRAYYAGLADGSIRRVDMYAVNPHSHEVEAVGGAGRIVTVAADGDPGAAFGHLQTGGGPHATVLAVTMDGMSLVSGDTQGRVFVADVATRQVVKAYSACKLAIAHLHVGTCSTAALAPGGHAEKTHRLLPPLKRVLAAGVLADHTVTVQLPAPRGRAVGFAAWVDAKAQQEFEFRRDTGDDAAPKDGPADVAAVQAKLNTVSAAYLALRETYGQLLQAHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFL3</accession>
    <name>A0A1A0HFL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_37067</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713272.1">
        <property type="nucleotide sequence ID" value="XM_018856816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFL3"/>
    <dbReference type="SMR" id="A0A1A0HFL3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFL3"/>
    <dbReference type="GeneID" id="30029792"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12 15" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291784" description="candidapepsin" evidence="13">
        <location>
            <begin position="17"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="14">
        <location>
            <begin position="75"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="40858" checksum="CAB602D054DB593A" modified="2016-10-05" version="1">MLPLSLLPLCLGVAHGLSLPRQEAENGPAPIALGFSVLKTVGNITAKEFWSQHSAERAKRDGYPIPIVDYRDISYRLDVYLGSDAQKSTVNLDTGSSDLWVYSSDYDPSTSSTSKDTGDAFSIGYLDGTSSAGEYYLDTLKFSTPNPVLSSFQFARSTSSSAYGVLGIADRNQERALSEYDNLPWALQAAGITPKASYSLFLGAEGGSGTVIFGGIDTEKYVGNLTKYSAQKSRGALAVNLQTMLVNGKTLSVDAPAILDSGTSLGLLPLEYMSELDSVFDTTVYTQGSIEYRLVSCDQPSDKYLDFDFGNNTIQIAYSDMVYHQGNDECLLGFGSYGSYMIFGDVFLRQAYVYYDLSDLTISLAQASYSSSSNIISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFL7</accession>
    <name>A0A1A0HFL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor SLU7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_35086</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SLU7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713261.1">
        <property type="nucleotide sequence ID" value="XM_018856569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFL7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFL7"/>
    <dbReference type="GeneID" id="30029545"/>
    <dbReference type="OrthoDB" id="249612at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030628">
        <property type="term" value="F:pre-mRNA 3'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021715">
        <property type="entry name" value="Slu7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039974">
        <property type="entry name" value="Splicing_factor_SLU7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942:SF2">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12942">
        <property type="entry name" value="STEP II SPLICING FACTOR SLU7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11708">
        <property type="entry name" value="Slu7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor SLU7" evidence="9">
        <location>
            <begin position="76"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="250"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="259"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="31734" checksum="21DF34F2D9657638" modified="2016-10-05" version="1">MALGSNNLYIPKYILDVPWYYKSGLKAEQAGAEDTLAHHRKTPGQAPVHHSEPQAGAGIFDEVTHVNGTKTRVADDYDAKRDRWHGHSADEWDEILTRWEAVKKTASRGQNMNEDSDDTDYELELEELGLSRSDLKTGHVEDPLEKSIRDRRDVPAYIRAINGNMGGKIRVGKDSTVGVVNEDSEFVKESADVAEFRQAQKFAWEKNKAFEAEQKKAHYDAQLASLKNPNVPIEEQPAADLNFSMEASPTMMMLQNRQNEQRRKDAGEAKRRRLMEMYGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFW7</accession>
    <name>A0A1A0HFW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_85264</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713377.1">
        <property type="nucleotide sequence ID" value="XM_018859245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFW7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFW7"/>
    <dbReference type="GeneID" id="30032220"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16982">
        <property type="entry name" value="CID_Pcf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045154">
        <property type="entry name" value="PCF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047415">
        <property type="entry name" value="Pcf11_CID"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921:SF3">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX 2 PROTEIN PCF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15921">
        <property type="entry name" value="PRE-MRNA CLEAVAGE COMPLEX II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="2">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="48441" checksum="98FACBBCF9DA0032" modified="2016-10-05" version="1">MNEEAFSAEYFQHLLSSLTLNSRALIAELTSLAERFVDNAQEIVELIDERIMKILPKYKLCSFYLMDSIIKNIGNPYNLLFATNLFKNFTESYLIIDDTPTRQNLINLFKTWVTGKTSAGLDIFPKETLLKVEKFIIQATSLTSSVQHESTRITRDMVLREGNYLLQYVISLDDEFEKAIDCTKKHEKNYEILRNCRLARNNLILEINLISECTMLESRDEFDRKKDHYVQRLREIRKVFDDQGKIQHGVLQIPKEVNSTPKDDLEAETILLNLTPKRIDVLSLLGETIDHAFESAVKLWGVHRFDRPILSVDLELVNNRTALNQEPEAVSGNLAGTLGISYDAVNFQGMGPEDQVKEIALDLSGFSYEDEGDGYDPELTFDDSDNKHSPPSSATNGVFVPGKSSLKRTNRSEERAVKRVRFEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGD6</accession>
    <name>A0A1A0HGD6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_102154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713710.1">
        <property type="nucleotide sequence ID" value="XM_018853714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGD6"/>
    <dbReference type="GeneID" id="30026690"/>
    <dbReference type="OrthoDB" id="10602250at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="32118" checksum="EE5607DF58FF7D84" modified="2016-10-05" version="1">MDQPVAFAPAPELLIFPDPYLGNPIIRDFLILRSQRNGGVESEVPTPIDNPSSCLSDSFLLSQDSVLSLGHTRPPPRDESPDSDPIVEPNGQALGGSLPSAEFAPILNTDSEELNFRTDRDATVFVPTRALSTLTRFGRLMEDLEVLQGIRVARFLDVADGESESESDENSFSELMLAVETQVTKAAPRNIVEWLAESFRQLRKTRPSSIDGIIMLHEASRAAHAANQKRQRMRWNSLRSQRACETSKSSNVFARLLKRWRRPKAPTPQPSFLINRLLKERFSNYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGQ0</accession>
    <name>A0A1A0HGQ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TATA element modulatory factor 1 TATA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_35598</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713538.1">
        <property type="nucleotide sequence ID" value="XM_018856631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGQ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGQ0"/>
    <dbReference type="GeneID" id="30029607"/>
    <dbReference type="OrthoDB" id="74178at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052602">
        <property type="entry name" value="Growth_transcription_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022092">
        <property type="entry name" value="TMF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022091">
        <property type="entry name" value="TMF_TATA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515:SF1">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46515">
        <property type="entry name" value="TATA ELEMENT MODULATORY FACTOR TMF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12329">
        <property type="entry name" value="TMF_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12325">
        <property type="entry name" value="TMF_TATA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA element modulatory factor 1 TATA binding" evidence="6">
        <location>
            <begin position="581"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="148"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="266"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="425"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="592"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="699" mass="77578" checksum="1A92CB0567E20E54" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGU8</accession>
    <name>A0A1A0HGU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Long chronological lifespan protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_76277</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713702.1">
        <property type="nucleotide sequence ID" value="XM_018858504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGU8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGU8"/>
    <dbReference type="GeneID" id="30031480"/>
    <dbReference type="OrthoDB" id="2234316at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23996">
        <property type="entry name" value="LCL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034543">
        <property type="entry name" value="LCL2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38425:SF1">
        <property type="entry name" value="LONG CHRONOLOGICAL LIFESPAN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="109" mass="12450" checksum="3B493F76BB932079" modified="2016-10-05" version="1">MFNFFNNQQQGQQQEPQFDFEGRVLESLCNEYLCPDTLACVALPKECPCKFPSSQLRCVLPNQEYICISKPAGNLEGKYDDPALNWKVDAKDDNVRDCGWVKRAWLGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGX1</accession>
    <name>A0A1A0HGX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_102616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713721.1">
        <property type="nucleotide sequence ID" value="XM_018853725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGX1"/>
    <dbReference type="GeneID" id="30026701"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="221" mass="24420" checksum="30966B62DCC956D7" modified="2016-10-05" version="1">MLLLMPTGGAESTMPSGGQWRDRSMVPLAKAAYMPATMSCQKAMLGDENSYYVISTKHPEEYGEKPLQSRATDLCNPGMDPGIIPMHRNRLSKSRATRHKMRVGLMENVLPLQDDHGWAARSEYCKAQSSQGSVGLGLASASCGVKSKTTKKNRRGARQNSGLTLSIYTDSTGITRLLFGECRRGLRLDTVAKMRVVIPMQRTCCWLVESRHGTRGPRCRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGY3</accession>
    <name>A0A1A0HGY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_29775</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713737.1">
        <property type="nucleotide sequence ID" value="XM_018855479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGY3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGY3"/>
    <dbReference type="GeneID" id="30028455"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000207">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000005">
        <property type="entry name" value="CTP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="315"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="575" mass="63530" checksum="A812667CC952C465" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHI8</accession>
    <name>A0A1A0HHI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADH:ubiquinone reductase (non-electrogenic)</fullName>
            <ecNumber evidence="3">1.6.5.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_34031</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a quinone + NADH + H(+) = a quinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:46160"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.6.5.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a ubiquinone + NADH + H(+) = a ubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:23152"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.9" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713952.1">
        <property type="nucleotide sequence ID" value="XM_018856432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHI8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHI8"/>
    <dbReference type="GeneID" id="30029408"/>
    <dbReference type="OrthoDB" id="3244603at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) (non-electrogenic) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015980">
        <property type="term" value="P:energy derivation by oxidation of organic compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.100:FF:000007">
        <property type="entry name" value="Rotenone-insensitive NADH-ubiquinone oxidoreductase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054585">
        <property type="entry name" value="NDH2-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706:SF47">
        <property type="entry name" value="EXTERNAL NADH-UBIQUINONE OXIDOREDUCTASE 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22366">
        <property type="entry name" value="NDH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="12">
        <location>
            <begin position="83"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="External alternative NADH-ubiquinone oxidoreductase-like C-terminal" evidence="13">
        <location>
            <begin position="482"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF22366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="60966" checksum="3103DB77DE10ACAE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHM1</accession>
    <name>A0A1A0HHM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_34666</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713858.1">
        <property type="nucleotide sequence ID" value="XM_018856508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHM1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHM1"/>
    <dbReference type="GeneID" id="30029484"/>
    <dbReference type="OrthoDB" id="369569at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045050">
        <property type="term" value="P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009445">
        <property type="entry name" value="TMEM85/Emc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19315">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06417">
        <property type="entry name" value="EMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017207">
        <property type="entry name" value="UCP017207_TM-p85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="160" mass="18175" checksum="E4CE618254B643A8" modified="2016-10-05" version="1">MTGDIPSPPGFEQEAPRLKKSDRTEKNEHTKKPTQKELDLLKVKKAWDIATMPAKNIPMNLVMSYMTGNSLQVIPIMMTLMLLWNPLKAILTETNSVFAGFTTNTNSLQMYLPKIVFVSCQLANMMIGLWKLNKLGLIPNKEADWLSWQVAGEYIEKLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHS6</accession>
    <name>A0A1A0HHS6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminopeptidase</fullName>
            <ecNumber evidence="11">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_76551</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9 11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714037.1">
        <property type="nucleotide sequence ID" value="XM_018858551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHS6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHS6"/>
    <dbReference type="GeneID" id="30031527"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF171">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="14">
        <location>
            <begin position="12"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="12">
        <location>
            <begin position="237"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="13">
        <location>
            <begin position="526"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="871" mass="98386" checksum="41936C9248B83AC8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHT4</accession>
    <name>A0A1A0HHT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_14895</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714051.1">
        <property type="nucleotide sequence ID" value="XM_018854555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHT4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHT4"/>
    <dbReference type="GeneID" id="30027531"/>
    <dbReference type="OrthoDB" id="271386at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00317">
        <property type="entry name" value="cyclophilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="35"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="234" mass="26035" checksum="24F78B8570DC8B37" modified="2016-10-05" version="1" fragment="single">LALAPEELKHLEGDPEVTHFAQFSIGLQKSETELEHLGDLKFALFGSVVPITVANFITSIEQEINGYTNSIIHRVVKGVIAQGGFLKKVAWKEYEPIAYERFPCENFQILHNKPGRISLASTQDDTNGMSFFISIPQSTLYFDNKYVVFGQLVLGFDVLQQISETPVNGETPIGEIVITKSVYMDPSSVPMVMDFGAVTGEYSLFIYGAIASAVLFAIYRLRNRRGLVDLKGFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHW4</accession>
    <name>A0A1A0HHW4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714081.1">
        <property type="nucleotide sequence ID" value="XM_018855538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHW4"/>
    <dbReference type="GeneID" id="30028514"/>
    <dbReference type="OrthoDB" id="4065753at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="544" mass="62248" checksum="94658C2865FB6C16" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHX4</accession>
    <name>A0A1A0HHX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_228569</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714091.1">
        <property type="nucleotide sequence ID" value="XM_018855208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHX4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHX4"/>
    <dbReference type="GeneID" id="30028184"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="423" mass="47406" checksum="CE2DD9351CE47B02" modified="2016-10-05" version="1">MIQEKFSEPTKRIYEESDVALFRSSLASQKLHHVISFLLEKVTAVEVPQGQLKSAIVTRNSNLEYPDVEDQKGGKAYTPKDNVANLVEIIDNLDKLVDETPPLKGPRRFGNMACRDWHTKVDSQSLSLLRKLSKDENYLIELNHYLVNAFGSKMRLDYGSGHELSFIAFIGGLFENGILNKDTTGEDVLAVFSRYYDLVRRLILDYSLEPAGSHGVWGLDDHFHFIYVLGAAEFNFRSGKNVKESFQYAPPVLQILSPHTISSYKLSNLYVNAIAFIFKLKLGPFHEHSPVLYDIHKSVTRWSKVQSGLLKMYEVEVFGKYPVVQHFWFGKGLYPWVDASTTKSLPVKSVDELEKSASKKDGKFPGMINGLQGMKTTSNNIAMTGAPWARNQPARGTPAARAVGNLGRSSSDIKGTFKGNNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI20</accession>
    <name>A0A1A0HI20_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714136.1">
        <property type="nucleotide sequence ID" value="XM_018855553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI20"/>
    <dbReference type="STRING" id="869754.A0A1A0HI20"/>
    <dbReference type="GeneID" id="30028529"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF7">
        <property type="entry name" value="DRUG_PROTON ANTIPORTER YHK8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="32"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="485" mass="54453" checksum="A2E48FFD023F46E6" modified="2016-10-05" version="1">MSLKALKEFEVSFDTLGDPEDALGLSHTRKWLIVIVVSMTSVCITSISSVWSLATEFIMSDYGVSHEVSTLGISLYIWFLGTGGIFLSPISEFHGRKIVYVSGLFVVFAFQFVPAFSSSFGFVLFSRAIAGFFASSFMSVASGTFSDLFTKAERTAHFKHDQDKELSKALILYSVSPFLGPGLGPLISGLICQHIDYRWQFKIMCIWSGLMTVLVLLFVPETYEPILLKKKAKRLRKSTGDQRYFAPIEMKNTSLYESIVVSSKRPLLLIFKDHMTMALCFYSGFILAIIYMFFVSFPYILSTVYQFNIQDQGLGFIGLILGLSLTALISPTLINKWHARLIAKNGGVHVTEFRFLPVMVGVFVAPIGLFIIAWTSYPDLHWIGPIFGSFVYGIGTILIFNGIFAYTVEAYRKYTASAMATNSFVRSIMSGIFPLFGLRMYEAMGIHWATTLLALFACILIPIPFLLFRYGARFRAKSPYTWSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI97</accession>
    <name>A0A1A0HI97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Fatty acid synthase subunit alpha</fullName>
            <ecNumber evidence="19">2.3.1.86</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="19">3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
                <ecNumber evidence="19">1.1.1.100</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">3-oxoacyl-[acyl-carrier-protein] synthase</fullName>
                <ecNumber evidence="19">2.3.1.41</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="27" type="ORF">METBIDRAFT_35283</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="27 28">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H(+) = a long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.86"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 19">Belongs to the thiolase-like superfamily. Fungal fatty acid synthetase subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.86" evidence="19"/>
    <dbReference type="EC" id="1.1.1.100" evidence="19"/>
    <dbReference type="EC" id="2.3.1.41" evidence="19"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714044.1">
        <property type="nucleotide sequence ID" value="XM_018856593.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HI97"/>
    <dbReference type="GeneID" id="30029569"/>
    <dbReference type="OrthoDB" id="4251012at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101026">
        <property type="term" value="P:mitotic nuclear membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900535">
        <property type="term" value="P:palmitic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00828">
        <property type="entry name" value="elong_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08950">
        <property type="entry name" value="KR_fFAS_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.20:FF:000005">
        <property type="entry name" value="Fatty acid synthase alpha subunit FasA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2490:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.25.70:FF:000001">
        <property type="entry name" value="Fatty acid synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040899">
        <property type="entry name" value="Fas_alpha_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047224">
        <property type="entry name" value="FAS_alpha_su_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026025">
        <property type="entry name" value="FAS_alpha_yeast"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041550">
        <property type="entry name" value="FASI_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050830">
        <property type="entry name" value="Fungal_FAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982:SF21">
        <property type="entry name" value="FATTY ACID SYNTHASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10982">
        <property type="entry name" value="MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18325">
        <property type="entry name" value="Fas_alpha_ACP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18314">
        <property type="entry name" value="FAS_I_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000454">
        <property type="entry name" value="FAS_yeast_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="KS3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52004">
        <property type="entry name" value="KS3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 22" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 19 23" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carrier" evidence="25">
        <location>
            <begin position="147"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Ketosynthase family 3 (KS3)" evidence="26">
        <location>
            <begin position="1117"/>
            <end position="1657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="596"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="603"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="active site" description="For beta-ketoacyl synthase activity" evidence="20">
        <location>
            <position position="1300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1767"/>
            <end position="1769"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1767"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1768"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1769"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1793"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="1803"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1812"/>
            <end position="1828"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1836"/>
            <end position="1839"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="1866"/>
            <end position="1868"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1867"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <position position="1868"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="23">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00060261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000454-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS52004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="OBA23563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1881" mass="204708" checksum="D92CBDD22CE1279A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIC5</accession>
    <name>A0A1A0HIC5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714118.1">
        <property type="nucleotide sequence ID" value="XM_018859478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIC5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIC5"/>
    <dbReference type="GeneID" id="30032453"/>
    <dbReference type="OrthoDB" id="4026708at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="406" mass="44341" checksum="F9FE5D626AE9255F" modified="2016-10-05" version="1">MSVLMSEPANQPDPPVRRKRGRPPKAEKPQDAFSTFTMQLESSPLAGGPNAELTSSQVLKGGEPDSYTPVMKVFPSPHCRKRRRSGNFSLPLDAPHILRIDELLAEESRSYTTSQGRNSLHTQVLEGLSNFVNDLSQSSSLQSISNDSALEPVIVLQHDSLSRISRRRVSVSPRDKPTVRSAGTCGENLDKTVKESPQATGPPFAVQPQYGAEEDLDLFGFELVVDETGQAALIDRLGMVFLSEVSKTSIATNTTHDNTGNLQSSHVIEDSGVGFHAAGRTHLLPTMSCAFDGESKGTNVSTDYLKSASCEALFGPGNLKHDRTELDSSISHLIELQAHDYGALNHVPEYFSGGLEETTHLYSERQGSNNGFSVDTQDLVIDRSRSDTPFESADARTALRRVFRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIG5</accession>
    <name>A0A1A0HIG5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_113252</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714281.1">
        <property type="nucleotide sequence ID" value="XM_018853959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIG5"/>
    <dbReference type="GeneID" id="30026935"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508836" evidence="2">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="125" mass="13744" checksum="9D6654A18B401316" modified="2016-10-05" version="1">MWVPIALGGCLFIVPGCPAFLISDSGFRFAVRKESHPCLRPWSRAAWPSAIPARALPGIPRQIPRGHCGRMERPSSGLALGPCIFCFGGWFFCLGGWCIFYLGGRCVFCLVGWFSCLVDWVFLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH5</accession>
    <name>A0A1A0HIH5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_113253</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714282.1">
        <property type="nucleotide sequence ID" value="XM_018853960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH5"/>
    <dbReference type="GeneID" id="30026936"/>
    <dbReference type="OrthoDB" id="10253709at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005371">
        <property type="term" value="F:tricarboxylate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015742">
        <property type="term" value="P:alpha-ketoglutarate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000053">
        <property type="entry name" value="Mitochondrial DNA replication protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000085">
        <property type="entry name" value="Tricarboxylate carrier, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053017">
        <property type="entry name" value="Mito_Cit/Oxoglu_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982">
        <property type="entry name" value="CITRATE/OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46982:SF1">
        <property type="entry name" value="CITRATE_OXOGLUTARATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="14"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="5">
        <location>
            <begin position="209"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="304" mass="32464" checksum="61D8604CF6D8B2CC" modified="2016-10-05" version="1">MAADAAQTIEKKPINFANILLGAGLNLAEVTTLGQPLEVVKTTMAANRSLSLVQAGKLVWSRGGVLGFYQGLIPWAWIEASTKGAVLLFVSAEAEYQYRSLGFSSFLAGMGGGVTGGVAQAYLTMGFCTCMKTVEITRAKQAAVAGAPQLTSFQVFKEIYRKEGIRGINKGVNAVAIRQMTNWGSRFGLSRLAEDGIKNLTGKGKDDRLNAVEKIAASCVGGGLSAWNQPIEVIRVEMQSKTADPNRPKNLGVFSAAKYIYSNNGVKGLYRGVAPRVGLGVWQTVFMVAFGDIFKKLLKTDGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIN2</accession>
    <name>A0A1A0HIN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_23456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714179.1">
        <property type="nucleotide sequence ID" value="XM_018855261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIN2"/>
    <dbReference type="GeneID" id="30028237"/>
    <dbReference type="OrthoDB" id="5580261at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018811">
        <property type="entry name" value="MRX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002:SF1">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10306">
        <property type="entry name" value="FLILHELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16324" checksum="53C7818C11156873" modified="2016-10-05" version="1" fragment="single">YDRHPILKRIPRFLRPYTTQFITAPVSHVTAFVVLHEITAVVPLIGIWYLLHQYHDTLMVSVLDLPSWATEKGTKIIDKALQDYDFGTFSIGEKLQLIKEGAYAYVIVKSLFPVRIAISILGMPWFAKWFVLPFTRIFSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIU8</accession>
    <name>A0A1A0HIU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Oxidoreductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_20092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714244.1">
        <property type="nucleotide sequence ID" value="XM_018854934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIU8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIU8"/>
    <dbReference type="GeneID" id="30027910"/>
    <dbReference type="OrthoDB" id="10064411at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039251">
        <property type="entry name" value="OXLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193:SF3">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase-like" evidence="1">
        <location>
            <begin position="85"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="196" mass="21943" checksum="74896EEE2BFBCA8A" modified="2016-10-05" version="1" fragment="single">RSKYLFYDLVKLSNRHPQHNSLPVSLLENIDGALAKEAKTARDEDASEARRKAAEAKIAKVFGGRIKGEDRKSSSRINVGKPRVIGGVKVPHRPLEPDNCCMSGCINCVWEMYNDDVKDWNTQRKKAAAALVAKGGVWPEDFHPPIELLKPENLPPSLALQARGNNPKGDEEPWDKVPVQIRVFAEMEKKIKAKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIZ7</accession>
    <name>A0A1A0HIZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_37232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714294.1">
        <property type="nucleotide sequence ID" value="XM_018856842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIZ7"/>
    <dbReference type="GeneID" id="30029818"/>
    <dbReference type="OrthoDB" id="441660at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF1">
        <property type="entry name" value="CYSTEINE RICH SECRETORY PROTEIN LCCL DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="512"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="669" mass="73612" checksum="71E1A82A99DF1CBC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ09</accession>
    <name>A0A1A0HJ09_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37089</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the THOC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714481.1">
        <property type="nucleotide sequence ID" value="XM_018856819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ09"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ09"/>
    <dbReference type="GeneID" id="30029795"/>
    <dbReference type="OrthoDB" id="340259at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040132">
        <property type="entry name" value="Tex1/THOC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22839">
        <property type="entry name" value="THO COMPLEX SUBUNIT 3 THO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19056">
        <property type="entry name" value="WD40_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="64"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="38006" checksum="4EBADE02E5F23301" modified="2016-10-05" version="1">MSIFTHLKHLSVKDFDRTGKILHSPILCVSVNAVGSRVINTRLDRSIRIWKFSSMALTDPVIIDNAHSNPVQSIAWLPTTETTFASAGGDLVVKIWRGNGSLERDVSVGGPGLGLKFTIVLFLPDGTLMLSATTNGLIYVYDVLDNYSEITHISITTHSGDIVNIQWTNRGHACFFVGLSTGPILIYTYDQGRLSKLHSLVGCRSVTCILVDPQGRYIAAGSKEGVVTFWSTADMVHSRALTSIDQEISSLSANKDGTFIFVGYGAGSNCRIFDTDSLTECCEIPTSAAGAESKLAVAWLASRNVLLYSQDLGRALGYARKEDRIEDDPQRKISHGYLKYGPRLALRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJG3</accession>
    <name>A0A1A0HJG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_35373</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714780.1">
        <property type="nucleotide sequence ID" value="XM_018856603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJG3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJG3"/>
    <dbReference type="GeneID" id="30029579"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="346" mass="41093" checksum="E3F9A1F3AC331ED0" modified="2016-10-05" version="1">MQGLSTSQDQQNLKPGDCPVPGERVKATFVSLARNLELYDLLPAIRNVEDRFNRKFHYDWIFLNDEPFSEEFKRVTGSIVSGTAKYGLVPKEHWGYPAWIDQEKAKKSRDDMEKARIIYGGSESYRHMCRYESGFFFRHPLMEEYDWYWRVEPGIKIHCDLDYDVFKFMEENGKDYGFTITIHEFEKTIPTLWQHTKDFIKLHPEHVAQDNLMKFVSNDNGETYNLCHFWSNFEVASLKFLRSKAYMDYFEYLDKQGGFFYERWGDAPVHSIAVSLFMPKNKIHFFEDVGYTHAPYTQCPLNDKFRADHKCDCKPESDFTFRGYSCGNQYYNAMGIEKPVEWEHFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJI8</accession>
    <name>A0A1A0HJI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent rRNA helicase RRP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent rRNA helicase rrp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_10235</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX47/RRP3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714533.1">
        <property type="nucleotide sequence ID" value="XM_018853719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJI8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJI8"/>
    <dbReference type="GeneID" id="30026695"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17954">
        <property type="entry name" value="DEADc_DDX47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044765">
        <property type="entry name" value="DDX47/Rrp3_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="96"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="294"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="453"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA24052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="480" mass="53096" checksum="58281AE4473DF92E" modified="2016-10-05" version="1">MAKNQPGKKSKLYSKSEETLALAEKIKQNALRAKLAQNKPATDGPSIAPEASEITVSDNGSKRFEHFSQLKLIPELLEAIQGMKFTKPTPIQAEAIPHALEGKDVIGLAVTGSGKTAAFAIPILQALWHAQLPYYGLVLAPTRELAYQIKETFDALGSGMGLRTCCIVGGMDMMDQARDLMRKPHVIVATPGRIVDHLENTKGFSLRNLKYLVMDEADRLLDLDFGPELDKILRVIPRERNTYLFSATMTNKIEKLQRASLQNPVRIAVSSKYATAENLVQLMMLVSDGYKNTYLIHLLNEFMGKSIIIFTRTCAHSQRTALLARILGFSAVPLHGHLSQSQRLGSLNKFKAGSANILVATDVAARGLDIPSVDVVINYDIPTDSKAYIHRVGRTARAGRSGKSISLVTQYDLEMYLRIESVLEKKLPKDPAPPKDVLDALYAHVDRATAEAIRQTKDFHDRGKGGRKQKSRDDADREEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJM5</accession>
    <name>A0A1A0HJM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714850.1">
        <property type="nucleotide sequence ID" value="XM_018858657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJM5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJM5"/>
    <dbReference type="GeneID" id="30031633"/>
    <dbReference type="OrthoDB" id="860at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF93">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN 25D9-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="212" mass="23686" checksum="BDD518CAB38A01E3" modified="2016-10-05" version="1">MTINARYLAALSKNPLLVKMATSGVLSSLNEISASIFSGQFQNKKLRLFGKEKTVSHPFSSKIGLMVIYGALMSTPVAHYYYGLLSKVFGGKQTSRVKLLQLVTTLCTLSPFLSGLYVSWLSIINSYQCSSEGLRTNLSRLVSVIKAGLKNNFWLVYRTSAITMLIALALAQNFVPPELWVVFTNFIFFVVGTVQNTKIKIRQRNERLKKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJP5</accession>
    <name>A0A1A0HJP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_36850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714702.1">
        <property type="nucleotide sequence ID" value="XM_018856788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJP5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJP5"/>
    <dbReference type="GeneID" id="30029764"/>
    <dbReference type="OrthoDB" id="6407164at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031138">
        <property type="term" value="P:negative regulation of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000047">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000064">
        <property type="entry name" value="Differentiation 1 negative regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000120">
        <property type="entry name" value="Negative regulator of differentiation 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF8">
        <property type="entry name" value="RNA-BINDING PROTEIN MRN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="110"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="248"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="339"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="460" mass="50890" checksum="E0FAAD44570F6F26" modified="2016-10-05" version="1">MAPQQQHSGASPDPSAPSRTVYLGNLPNDVEPNELLDYVRSGILENVKILPSKNCAFISFIDLQSALLFHSDCILKKLSIKGNDIRVGWGKNTPILPAIKDAIQKDGATRNVYLGNLHNPATDEMITEDELRADLSSYGVIDCIKIIHEKGIAFIHFLSILSAIRCVSNLPLEEKYLDKKCFYGKDRCAFITKTQQHNAAQYLGLAPGMEHVVTAADREFISSALVQQSAAAAQIATQAGGANNLGNRTVYLGNLHQESSVEEICNVVRGGLLQSIRFLKERHVCFITFIDPIAAAQFFAMCQLHGLTIHNRRIKVGWGKHSGPLSNALSLAVSNGASRNIYIGNINDFAYFNAQKLRDDFSTFGEIEQINYLEAKNCAFINFVNIANAIKAIDGIKSFKDYKTLKINFGKDRCGNLPRQFQNQAQNLVAESLDTRQFGNVNQAQFSRGTSASPDREYRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJR0</accession>
    <name>A0A1A0HJR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome maturation protein SDO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_36439</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714717.1">
        <property type="nucleotide sequence ID" value="XM_018856738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJR0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJR0"/>
    <dbReference type="GeneID" id="30029714"/>
    <dbReference type="OrthoDB" id="10253092at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990932">
        <property type="term" value="F:5.8S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1250.10:FF:000001">
        <property type="entry name" value="SBDS, ribosome maturation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018023">
        <property type="entry name" value="Ribosome_mat_SBDS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF1">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01267">
        <property type="entry name" value="UPF0023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="10">
        <location>
            <begin position="111"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="11">
        <location>
            <begin position="175"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="253" mass="28838" checksum="ABF7DA3B61571B45" modified="2016-10-05" version="1">MGMINQPSGQIRLTNVSLVKIKKGKKRFEIACYQNKVQDWRLRVEKDLDEVLQIPLVFTNVSKGQVANNEDLLNAFGTTNHDEILLEILDKGEIQLNEKERSANLQQKQNEFLNLILTKCINPRSKKRYPPSMIQKALGQLKYHLNPTKATKLQALDAIKLLISAQVIPIARAQMKVKITVLLRVRDAFQEHIRPMVHRIESENEGAKLFEAVAIIDPSHYKEIVDLLLGEDPDKMKRGEGSVEVLDMVALQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK29</accession>
    <name>A0A1A0HK29_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_121046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714723.1">
        <property type="nucleotide sequence ID" value="XM_018854169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK29"/>
    <dbReference type="GeneID" id="30027145"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="210" mass="23559" checksum="80AB11F2C53FE416" modified="2016-10-05" version="1">MLVFNSSYKLSCHGIHSTYLPSSHQTRGRSSERCTQLLPFSPAFTFLTVQVTTVLHSQHATQKHRSPPVHHPSQSPPLIIRLLSCLLSYQNISSACGCQTVFKFGQPCADCIWAEGNQSACHFFCTTQEIKILGREWHFSLRLISVSTSALPPHHPLPGIKTLCVIRSQFCSKISSVETYRKAQANARLLIFELLRTSHSRSLKAAWPFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKE4</accession>
    <name>A0A1A0HKE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleotide-diphospho-sugar transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714754.1">
        <property type="nucleotide sequence ID" value="XM_018856823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKE4"/>
    <dbReference type="STRING" id="869754.A0A1A0HKE4"/>
    <dbReference type="GeneID" id="30029799"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="566" mass="64343" checksum="8DC0A4000FC5FB53" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKI8</accession>
    <name>A0A1A0HKI8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_35633</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714888.1">
        <property type="nucleotide sequence ID" value="XM_018856636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKI8"/>
    <dbReference type="STRING" id="869754.A0A1A0HKI8"/>
    <dbReference type="GeneID" id="30029612"/>
    <dbReference type="OrthoDB" id="4310724at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17946">
        <property type="entry name" value="DEADc_DDX24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="208"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="459"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="92"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="11">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="100"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA24407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="756" mass="84051" checksum="96069A27E505D93F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKW5</accession>
    <name>A0A1A0HKW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CAP-Gly domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10587</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714929.1">
        <property type="nucleotide sequence ID" value="XM_018853808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKW5"/>
    <dbReference type="STRING" id="869754.A0A1A0HKW5"/>
    <dbReference type="GeneID" id="30026784"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="2">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="706"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="829" mass="93954" checksum="15BCF968C62CD11E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ55</accession>
    <name>A0A1A0GZ55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_14028</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HUS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709333.1">
        <property type="nucleotide sequence ID" value="XM_018854472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ55"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ55"/>
    <dbReference type="GeneID" id="30027448"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016580">
        <property type="entry name" value="HUS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011312">
        <property type="entry name" value="Cell_cycle_HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR011312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA17036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="338" mass="38313" checksum="9F613D2CA3740E3E" modified="2016-10-05" version="1">MAQILATRTGTCGTENENTASSESMKLKLETQSSEVLKNCFSLINTLRKRVILRFSPEQLLAILINDSSLNQEPQIWCKFPMRNIFLRIEIQSLRDNWILLEINIELFLQALRNFDKASSTELKIRLQKKEGQKSSGSAYLALQYSDITENSSTINHVFRIPVRILKEAAGLLKEPVLPRIDLMIKLPNEFFATYRRLERFKNSTAHEKMTISASQRNGGTLKFVLQEADSCNTTIKWNGRLDIQVPPSDSEYDSLRAAALQESPSKNHEDRSAIGVDVTVNLKEWCLTAKIVGTCQTVILFICDNNACAIHCLLDETDDAEIIYYISGIRQAEPYSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1P2</accession>
    <name>A0A1A0H1P2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DUF1977-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_225669</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709344.1">
        <property type="nucleotide sequence ID" value="XM_018855173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1P2"/>
    <dbReference type="STRING" id="869754.A0A1A0H1P2"/>
    <dbReference type="GeneID" id="30028149"/>
    <dbReference type="OrthoDB" id="1507364at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF3">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="81"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="92"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="168"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA17949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="319" mass="35659" checksum="78CA6734AAACF532" modified="2016-10-05" version="1">MSSHSAEQEKVVLRVLSCKPHQYYEILECEKSASDSTIKKSYRKLAIKLHPDKNSHPRASEAFKYLNKAWGVLSDPSKKRIFDQTGSDPDSRFAGSSSPGAGATSGFSRGFGNAGGAGAQGFEDDIFNMFFGGGAAPGHTTFTFGGNNGFTFQSFGNGFDPFAGNFAQQQRQRQSQRQSQQNRAEPTSWETLRQLAPVLLVLLATLLSSLFSGDSAPEYSFTKSSKFPVRRNTPTFEIPYFVSESFVKDKNGRALHNFDTKVENTYILDKRARCRREQTRRREMIQDAQGWFFTDEKRMNQARNMPMPACSELKGMGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1P6</accession>
    <name>A0A1A0H1P6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_47873</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709346.1">
        <property type="nucleotide sequence ID" value="XM_018858234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1P6"/>
    <dbReference type="STRING" id="869754.A0A1A0H1P6"/>
    <dbReference type="GeneID" id="30031210"/>
    <dbReference type="OrthoDB" id="10266508at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097308">
        <property type="term" value="P:cellular response to farnesol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd16922">
        <property type="entry name" value="HATPase_EvgS-ArcB-TorS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000001">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1530:FF:000002">
        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Two-component osmosensing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45339:SF1">
        <property type="entry name" value="HYBRID SIGNAL TRANSDUCTION HISTIDINE KINASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18947">
        <property type="entry name" value="HAMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="53"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="147"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="239"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="331"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="423"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="497"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="14">
        <location>
            <begin position="866"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1018"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1018"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1035"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="11">
        <location>
            <position position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA17951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1085" mass="118452" checksum="577C70F8DDD9FB48" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1R7</accession>
    <name>A0A1A0H1R7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_226333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709366.1">
        <property type="nucleotide sequence ID" value="XM_018855178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1R7"/>
    <dbReference type="STRING" id="869754.A0A1A0H1R7"/>
    <dbReference type="GeneID" id="30028154"/>
    <dbReference type="OrthoDB" id="5344687at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA17971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="132" mass="15177" checksum="3B146BF3F9AD73E3" modified="2016-10-05" version="1">MVRTSPFEQLIVRITYYFHMNETDTPDALLQQVPLIRNPAFQPPRPITLNTDYAKLLATIPGSVMPPKAAISREELQAWTTDLEAVERNDLNMEARDKDIAVYKEWLVKVQRKVAPGFDYEIMTPTKNGDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Z0</accession>
    <name>A0A1A0H1Z0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_13886</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709365.1">
        <property type="nucleotide sequence ID" value="XM_018854442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Z0"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Z0"/>
    <dbReference type="GeneID" id="30027418"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000191">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA17970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="148" mass="16799" checksum="AAADDBA138295761" modified="2016-10-05" version="1">MSGILFEDMFVIDSVDQARYNKVSRIEGQSSTSQEVKITLDINSELFPVKDNDSLTITLASSLGNESSMMTSNGSWRPPKRGDRSLADDYDYVMYGTVYKFEESSENDKMAVYISFGGLLMRLEGGYRSLSNLKQENAYILIRHYKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H236</accession>
    <name>A0A1A0H236_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_226805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709413.1">
        <property type="nucleotide sequence ID" value="XM_018855187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H236"/>
    <dbReference type="GeneID" id="30028163"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="204" mass="22103" checksum="820F5D8C1CB238BB" modified="2016-10-05" version="1">MSFFCPSCLYFVLHVSIFCPSCLYFRSLISPLSVFLVCLFSPNVAPLVLSSIDPPDTSPQPSLPHIIPARARGKHCALPPALFFPANIERLAPAKSLPLHTSTPPVKACLSEPQAQCRSSPCSRRHPLVPPFPNPRFLCTLPALFSGAPGAGLVLLRNLVWVPPTIYSPHSCHVLRAAGPSKRRLDRHPVPGLACLPVFCLDCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H291</accession>
    <name>A0A1A0H291_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleolar protein 58/56 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_230741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709465.1">
        <property type="nucleotide sequence ID" value="XM_018855257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H291"/>
    <dbReference type="STRING" id="869754.A0A1A0H291"/>
    <dbReference type="GeneID" id="30028233"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar protein 58/56 N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="128" mass="14612" checksum="425D0009CA43D4BD" modified="2016-10-05" version="1">MYYCGYIAVRLREIQDALLDLGKFCKMIELVLFAFFKGAVQALENAKDVSEMRELLWVFRSKTWVTPYVDSLFNNIAQGFSKYIRVHGLKLLKDSQEGVIERAKLGLGHAFSKVKVKFTVQKNGANIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H500</accession>
    <name>A0A1A0H500_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ARM repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709560.1">
        <property type="nucleotide sequence ID" value="XM_018858142.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H500"/>
    <dbReference type="GeneID" id="30031118"/>
    <dbReference type="OrthoDB" id="200660at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF0">
        <property type="entry name" value="PRECOCIOUS DISSOCIATION OF SISTERS 5, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1194"/>
            <end position="1283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1199"/>
            <end position="1219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1221"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1262"/>
            <end position="1283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1283" mass="145511" checksum="512964C4786FA2D9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H582</accession>
    <name>A0A1A0H582_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase MRP protein 1 RNA binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709602.1">
        <property type="nucleotide sequence ID" value="XM_018856377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H582"/>
    <dbReference type="STRING" id="869754.A0A1A0H582"/>
    <dbReference type="GeneID" id="30029353"/>
    <dbReference type="OrthoDB" id="5414547at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000294">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, RNase MRP-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047205">
        <property type="entry name" value="RMP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047204">
        <property type="entry name" value="RMP1_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37792:SF1">
        <property type="entry name" value="RIBONUCLEASE MRP PROTEIN SUBUNIT RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20945">
        <property type="entry name" value="RMP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="RNase MRP protein 1 RNA binding" evidence="2">
        <location>
            <begin position="24"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="14596" checksum="9ED85714A475B573" modified="2016-10-05" version="1">MTVKTSQQIFSPEIVQKLENEYGILHLICHRNRNQHRVAVWWKDLNQLHRSVRKILRAVYHFEEVTKYAIKEKLHAEALGVACSMIKKRLSQDAFILLMGLWHLGSSSVWGWHWWRVSVLSIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5A8</accession>
    <name>A0A1A0H5A8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_46583</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF2B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709667.1">
        <property type="nucleotide sequence ID" value="XM_018858056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5A8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5A8"/>
    <dbReference type="GeneID" id="30031032"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990856">
        <property type="term" value="F:methionyl-initiator methionine tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000002">
        <property type="entry name" value="Translation initiation factor 2, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="10">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="33893" checksum="1781DE707C8CBDB0" modified="2016-10-05" version="1">MSSSNCRFYENKYPEVDEVVMVNVQEIAEMGAYVKLLEYDNIEGMVLLSELSRRRIRSIQKLIRVGKNEVAVVLRVDKEKGYIDLSKRRVSAEDIVKCDERYNKLKAVHLILRHCAEKYMVLLETLYQTVGWPLLRKYGHAYDAFKLLITDPSIFADVEAPYPEILEELKLYISRRLTPQAIKVRADVEVSCFAYEGIDAIKTALKAAELELTEQMQVKAKLVAAPLYVISTQALDKNQGVALLEKAIEKVSASIEAAGGNLKITMAPKAVTATEDAELQALLERKEADEKDTSDEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5D2</accession>
    <name>A0A1A0H5D2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_80055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709652.1">
        <property type="nucleotide sequence ID" value="XM_018859165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5D2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5D2"/>
    <dbReference type="GeneID" id="30032141"/>
    <dbReference type="OrthoDB" id="272977at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090522">
        <property type="term" value="P:vesicle tethering involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039682">
        <property type="entry name" value="Sec8/EXOC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007191">
        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048630">
        <property type="entry name" value="Sec8_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14146:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20652">
        <property type="entry name" value="Sec8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04048">
        <property type="entry name" value="Sec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec8 N-terminal" evidence="5">
        <location>
            <begin position="31"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec8 middle helical bundle" evidence="6">
        <location>
            <begin position="321"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1092" mass="125354" checksum="605E7C1873A2B8D2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Q1</accession>
    <name>A0A1A0H5Q1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">AAA-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_79874</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709893.1">
        <property type="nucleotide sequence ID" value="XM_018859141.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H5Q1"/>
    <dbReference type="GeneID" id="30032117"/>
    <dbReference type="OrthoDB" id="5421at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140674">
        <property type="term" value="F:ATP-dependent histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000219">
        <property type="term" value="P:positive regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006418">
        <property type="term" value="P:tRNA aminoacylation for protein translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05491">
        <property type="entry name" value="Bromo_TBP7_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19517">
        <property type="entry name" value="RecA-like_Yta7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001218">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF0">
        <property type="entry name" value="TAT-BINDING HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="17">
        <location>
            <begin position="1249"/>
            <end position="1291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="234"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1363"/>
            <end position="1387"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1319"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="333"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="392"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="424"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA19361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1519" mass="171323" checksum="BB69E3F3620E9DBC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Y3</accession>
    <name>A0A1A0H5Y3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709897.1">
        <property type="nucleotide sequence ID" value="XM_018858059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Y3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Y3"/>
    <dbReference type="GeneID" id="30031035"/>
    <dbReference type="OrthoDB" id="25503at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="1">
        <location>
            <begin position="643"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="827" mass="94718" checksum="3610266428049088" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H638</accession>
    <name>A0A1A0H638_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome biogenesis protein RLP24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_16050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709904.1">
        <property type="nucleotide sequence ID" value="XM_018854622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H638"/>
    <dbReference type="STRING" id="869754.A0A1A0H638"/>
    <dbReference type="GeneID" id="30027598"/>
    <dbReference type="OrthoDB" id="10262490at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="5">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="147"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="189" mass="22380" checksum="C1BCA7194BA3553E" modified="2016-10-05" version="1" fragment="single">MRIYSCHFCSSPVYPSKGIMFVRNDAKEFRFCRSKCHKAFKQRRNPRKLRWTKAFRKAAGKELVVDSTLTFAAKRNVPVRYNRELVATTLKAMSRIEEIRLKRERAFYKNRMRGNKERQLASDRKLVESNPELLRMRDVELRKKAEKLAAAEAESDDEDEDMMDVESEEEESDEELKQKVVLKAKRKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H645</accession>
    <name>A0A1A0H645_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ML-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_79519</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor potential (TRP) ion channel family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710029.1">
        <property type="nucleotide sequence ID" value="XM_018859079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H645"/>
    <dbReference type="STRING" id="869754.A0A1A0H645"/>
    <dbReference type="GeneID" id="30032055"/>
    <dbReference type="OrthoDB" id="5212126at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032800">
        <property type="entry name" value="TRP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF2">
        <property type="entry name" value="FLAVIN CARRIER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14558">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01320">
        <property type="entry name" value="TRP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="420"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="568"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="ML-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="654"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="664"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="696"/>
            <end position="705"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="721" mass="79704" checksum="5396E3DBBE5B7E97" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L9</accession>
    <name>A0A1A0H6L9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710259.1">
        <property type="nucleotide sequence ID" value="XM_018854265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6L9"/>
    <dbReference type="GeneID" id="30027241"/>
    <dbReference type="OrthoDB" id="10457577at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="185" mass="20338" checksum="3460D49FC2187717" modified="2016-10-05" version="1">MAQTLGELGYVKSDKFTEEVQENLSANQLSDIDVVPEKHGNQRTCKIISHKDGDKKGHEIVLPSEMSDRIENDKNAAAKLDGKTEDNKGKFEGKTEGNVGKFEGKTEGITEGSVDKFEGKTQCKTEGKIEGKSEGKFEGKTEGKFEGKTEGKTEGTTEHTRRYAISDSEEHEVWLKATPAVVCEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6N4</accession>
    <name>A0A1A0H6N4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein mS33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45999</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710214.1">
        <property type="nucleotide sequence ID" value="XM_018857980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6N4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6N4"/>
    <dbReference type="GeneID" id="30030956"/>
    <dbReference type="OrthoDB" id="2257454at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013219">
        <property type="entry name" value="Ribosomal_mS33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13362:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08293">
        <property type="entry name" value="MRP-S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="101" mass="11645" checksum="E410BD9CAF20B857" modified="2016-10-05" version="1">MSAFRKINLNQLKQVQKLSAEIFGETYNPTGIRNGSEVLRAPLRGKEIASYYGVNESAPTFKDFKKWFPELNFIDPKETYRMKMVIDRKKRGKGAPKKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6T3</accession>
    <name>A0A1A0H6T3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase RBP11-like dimerisation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33302</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710193.1">
        <property type="nucleotide sequence ID" value="XM_018856264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6T3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6T3"/>
    <dbReference type="GeneID" id="30029240"/>
    <dbReference type="OrthoDB" id="10248581at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06926">
        <property type="entry name" value="RNAP_II_RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037685">
        <property type="entry name" value="RBP11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="120" mass="13783" checksum="25138382F12815C2" modified="2016-10-05" version="1">MNAPDRFELFILPDGVEKIKIIPDTKVPNAAIIKIEREDHTLANMLRAHLAKDDRVIFAAYKVEHPLFANFVLRIQTDDDYTPRDALKNACSSLISELDIMKTKFKDEWALKSLLNNNDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U3</accession>
    <name>A0A1A0H6U3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile)-lysidine synthetase</fullName>
            <ecNumber evidence="1">6.3.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_26581</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + L-lysine + ATP = lysidine(34) in tRNA(Ile2) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710156.1">
        <property type="nucleotide sequence ID" value="XM_018855321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U3"/>
    <dbReference type="GeneID" id="30028297"/>
    <dbReference type="OrthoDB" id="434144at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032267">
        <property type="term" value="F:tRNA(Ile)-lysidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033:SF1">
        <property type="entry name" value="TRNA(ILE)-LYSIDINE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="7">
        <location>
            <begin position="5"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="380" mass="44671" checksum="3D0D3E5DA7B042DA" modified="2016-10-05" version="1" fragment="single">IPDRVLLALSGGADSMCLAYLLAQYKKQSRLLMLISAITIDHGFRQESAAEAKKVGDIVLLWGIQHHVEKLRYLRDVHSITNFEEIARDLRYNVFRKKCADYGTNALLVAHNKDDQLETFLQRLQMNSTLFGLVGLRERSPLPTRDISPLKDEKVYIYRPLLHYKKAEIFQTCKSLGVPWFEDHSNQDIHLTKRNLLRYIINEYVPQALDARPELTCITRKSLTATTQEIESFVHGLEIQKLCFDLRVKSHQFQFDKVKAEVKFSIPFHEIMGVQQLVFSRWLYETIYPLSSSKNFHWSYAKLERQAIPRLWTWLQNPQNRLTMTYLNVKLDINPEGQDVNFLVSRQAPQRDVVHNYFVSVVPGDCMWHLFDRLWWLKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7A7</accession>
    <name>A0A1A0H7A7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Mitochondrial import inner membrane translocase subunit TIM50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_59048</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the TIM23 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TIM50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710504.1">
        <property type="nucleotide sequence ID" value="XM_018858304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7A7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7A7"/>
    <dbReference type="GeneID" id="30031280"/>
    <dbReference type="OrthoDB" id="287041at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046902">
        <property type="term" value="P:regulation of mitochondrial membrane permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000019">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FCP1 homology" evidence="15">
        <location>
            <begin position="188"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="469" mass="53550" checksum="63007266405DC6AC" modified="2016-10-05" version="1" fragment="single">MLPTLLRATKTIVSRSGVTVGASRSASVGVSFTSTVPISKRFYSDKKASEKADDKPQSILNEDMLSRAGFEEAEKEKSSEEETEGKKDDTDRSARRRRRAQTSKDRQREKYANWFYTATLFGGFAGLGFLCRDWDSEDEQKAVEGQGIENGFTPQLMYGRLNKRVSSLFTFFSEPAFENLLPPPPPEAYRRPLTLVLTLDDLLVHSSWDAKNGWRTAKRPGLDYFLGYLSQYYEIVIFGSEYQMYSEKTVAKLDPLRAYIQYALFREACRYKDGKLIKDLNLLNRDLANTIAIDVNEETLVLNPENAITLKPWDGKPDDTLVKLIPLLEYLATQPVKDVRPILSSFTDKSSIVEEYSAREAKLRQKWMEDNKHLLERAHKPNAGSFIASLMGIPQSSVNKQPKMPLDIIREHGQLQYESLQRYLKENEAKFLEEEKKMKEEYGKVTLNKLMTEGMPNPEDIAKAQAEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7G6</accession>
    <name>A0A1A0H7G6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="13">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_211671</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Oxidizes long-chain alcohols and, in the presence of glutathione, is able to oxidize formaldehyde. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>S-(hydroxymethyl)glutathione + NAD(+) = S-formylglutathione + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-(hydroxymethyl)glutathione + NADP(+) = S-formylglutathione + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>S-nitrosoglutathione + NADH + H(+) = S-(hydroxysulfenamide)glutathione + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:78371"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:145544"/>
            <dbReference type="ChEBI" id="CHEBI:229723"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10736"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15734"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a secondary alcohol + NAD(+) = a ketone + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17087"/>
            <dbReference type="ChEBI" id="CHEBI:35681"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710564.1">
        <property type="nucleotide sequence ID" value="XM_018855042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7G6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7G6"/>
    <dbReference type="GeneID" id="30028018"/>
    <dbReference type="OrthoDB" id="417550at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033833">
        <property type="term" value="F:hydroxymethylfurfural reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106322">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106321">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080007">
        <property type="term" value="F:S-nitrosoglutathione reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000947">
        <property type="term" value="P:amino acid catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033859">
        <property type="term" value="P:furaldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000001">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF12">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE CLASS-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0520">NAD</keyword>
    <keyword evidence="5 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="15">
        <location>
            <begin position="34"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="14">
        <location>
            <begin position="205"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="40127" checksum="77EC261AA6C837E9" modified="2016-10-05" version="1">MSTTGKTITCKAAVAWEAGKPLSIEEITVDPPKAHEVRIKITDTGVCHTDAYTLSGVDPEGAFPAILGHEGGGIVESVGEGVTNVKVGDHVIALYTAECKECKFCKSGKTNLCGAVRETQGKGVMPDGTSRFKCNGKELFHFMGCSTFSQYTVVADVSVVAINEKAPLESVCLLACGVTTGYGAATVTANVQEGDTVAVFGAGCVGLSVIQGAASRKAKKIIVVDINNSKKEWAEKFGATDFVNPKELPEGTTIVQKLIEMTDGGCDFTFDCTGIVEIMRQALEACHKGWGQSIIIGVAAAGKEISTRPFQLVTGRVWKGSAFGGVKGRTQLPGMVEDYMNGKLMVDEFITHRHTLDKINTAFDDMHDGNCVRAVINM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7S0</accession>
    <name>A0A1A0H7S0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_212107</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710596.1">
        <property type="nucleotide sequence ID" value="XM_018855054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7S0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7S0"/>
    <dbReference type="GeneID" id="30028030"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032440">
        <property type="term" value="F:2-alkenal reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:quinone reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05286">
        <property type="entry name" value="QOR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047618">
        <property type="entry name" value="QOR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE PIG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48106:SF13">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="17"/>
            <end position="330"/>
        </location>
    </feature>
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            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="333" mass="36343" checksum="73B8EDCC1B53F82C" modified="2016-10-05" version="1">MSIPTTQNVALARKQSDSPDGIEYAEVDTPQITDPHDMIIKNKYAGVNFIDAYFRKGIYPTEFPHIFGKEASGVIVAVGEAVTSYKVGDKVAYMAAKSFAQYTKVSDDAVQIKKLPDDSTDEDLKKWAAVLLQGLTAITLSIESYKVEKGDFILVWAAAGGVGQILTKLTSSLGARVIAIASTDDKLRTAKAKGAEFLIKVDENIPARVAEITNGQGVRASFDSVGKDSFEDSFASVGRKGTLVSYGNASGVVPPFSINRLSSKNMKITRSVLFNSVQTKEEWVYYTDILEDALRRDTVDVTIYKTYPLSEYSQAMKDLEGRKTSGKLVLEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7W1</accession>
    <name>A0A1A0H7W1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_45395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710636.1">
        <property type="nucleotide sequence ID" value="XM_018857913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7W1"/>
    <dbReference type="GeneID" id="30030889"/>
    <dbReference type="OrthoDB" id="4074802at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="59" mass="6666" checksum="36FE36DAA62C2E51" modified="2016-10-05" version="1">MAHSPPLNIYAITAMGTFVATYLWIKSPLKYRGASENRLSPKADLAWREGAQKWLDNKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H848</accession>
    <name>A0A1A0H848_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">WHIM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710592.1">
        <property type="nucleotide sequence ID" value="XM_018854304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H848"/>
    <dbReference type="STRING" id="869754.A0A1A0H848"/>
    <dbReference type="GeneID" id="30027280"/>
    <dbReference type="OrthoDB" id="349045at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="687"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="516"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="722"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="735"/>
            <end position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="761" mass="85185" checksum="596927282A9091BC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C2</accession>
    <name>A0A1A0H8C2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DDE-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_18009</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710662.1">
        <property type="nucleotide sequence ID" value="XM_018854765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8C2"/>
    <dbReference type="GeneID" id="30027741"/>
    <dbReference type="OrthoDB" id="125347at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050863">
        <property type="entry name" value="CenT-Element_Derived"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004875">
        <property type="entry name" value="DDE_SF_endonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006600">
        <property type="entry name" value="HTH_CenpB_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303:SF73">
        <property type="entry name" value="PROTEIN PDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19303">
        <property type="entry name" value="TRANSPOSON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03184">
        <property type="entry name" value="DDE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03221">
        <property type="entry name" value="HTH_Tnp_Tc5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00674">
        <property type="entry name" value="CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51253">
        <property type="entry name" value="HTH_CENPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH CENPB-type" evidence="2">
        <location>
            <begin position="64"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="537" mass="61356" checksum="3D78211C83C01FF6" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8K2</accession>
    <name>A0A1A0H8K2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Nucleotide-diphospho-sugar transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_20025</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710842.1">
        <property type="nucleotide sequence ID" value="XM_018854922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8K2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8K2"/>
    <dbReference type="GeneID" id="30027898"/>
    <dbReference type="OrthoDB" id="4484309at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31646:SF1">
        <property type="entry name" value="ALPHA-1,2-MANNOSYLTRANSFERASE MNN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="602" mass="69616" checksum="60886D2DD679C060" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8M2</accession>
    <name>A0A1A0H8M2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_32258</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710755.1">
        <property type="nucleotide sequence ID" value="XM_018856018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8M2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8M2"/>
    <dbReference type="GeneID" id="30028994"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="461"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="11">
        <location>
            <begin position="72"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="575" mass="62639" checksum="4C9DEFD3C8D43427" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8T6</accession>
    <name>A0A1A0H8T6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_195843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710820.1">
        <property type="nucleotide sequence ID" value="XM_018854873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8T6"/>
    <dbReference type="GeneID" id="30027849"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="349" mass="37530" checksum="4F03A7B014FBF351" modified="2016-10-05" version="1">MIAQVIFCFRLINCVSEIAGLCLTGYFLVGLLLELSVQAESTTMDLNVVQIKEYYSGPFGAEPEIQNHSGNIYADFIPVHIVAASAAPHPKYSKTTETDCSFVSSPSSDGSILELSDGDTGASSVLSFNSDSKLGSSAGSSMKPAVVVDYIETDEIKRTRGILIPDNNSIFNGESKISKVETTALLPAANPDIDMESGWQVQGRKRQNCHRRTGTKVETVSRTSGKKLASGPESPVSQSSLSKNPFLVLADIDENDYQEPRPSRPHKKVPGNLELSSLENVSPGISAAMDLAVDNYEALTKNSVTNSMDIACETIDEKALGLSQDLLEGSPPDAEEDPHMGSKKSRNYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H912</accession>
    <name>A0A1A0H912_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_43353</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711018.1">
        <property type="nucleotide sequence ID" value="XM_018857649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H912"/>
    <dbReference type="STRING" id="869754.A0A1A0H912"/>
    <dbReference type="GeneID" id="30030625"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="187" mass="20690" checksum="757559878CC5D166" modified="2016-10-05" version="1">MVRPVVISGPSGTGKSTLLKKLLAEFPDTFGFSVSNTTRQPRQGEVDGVDYHFSTVDEFKKLIAEEKFIEWAQFSGNYYGTSIKAVDDVAKLNRICILDIDMQGVKSVKKAQIDARFLFLSPPSIASLRDRLSGRGTETEDSIAKRIAAASAEMEYAKTGAHDKVIVNDDLERAYAEFKEFILAEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H933</accession>
    <name>A0A1A0H933_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_201841</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711156.1">
        <property type="nucleotide sequence ID" value="XM_018854942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H933"/>
    <dbReference type="GeneID" id="30027918"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="68" mass="6833" checksum="404F0FF749AF5936" modified="2016-10-05" version="1">MSLQGKLPERPGPTPRGHASSASLGAVGCPWSPIGGSEVSAGSSCLIQAGPGSPRVHHNRCASVRCVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H991</accession>
    <name>A0A1A0H991_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_78823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710965.1">
        <property type="nucleotide sequence ID" value="XM_018858948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H991"/>
    <dbReference type="GeneID" id="30031924"/>
    <dbReference type="OrthoDB" id="4005225at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="384" mass="44443" checksum="4B8C0B8E176755C4" modified="2016-10-05" version="1">MRLIFNKYKSTKTDSLSLAGALGLLAPHKDITKSTTYKPSLFRNLQKGSVSEEYAEFKHQQPISFEGPSAVHQKLIANLMSDLRHATEFVSWPKKLPAIKNLNVHVKSLTNEAELLDLLRSSYIEKQLSLPLLMRFLLNKHLKDLTKLPFDVANINRSEFTKHGWSNINFVELKILLLKKYHDLQQPLEIVKQLKLSFGTEFLPLIRKHQLLPFYERIVWKFFFEYIGMGEEKAIIEELNSLKSSFLVWEATTARNGEILTKILHQHQLNTPQMLFVKVASCDLVQRSIEAQLAHGRSALLSALKQTSWKFKVYKSMDTNEVMARAYVFSLIHNLEDILTTHFPDSAGFPELQKILMDLKAERERMIKPEAMELDYECEVLYAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9C2</accession>
    <name>A0A1A0H9C2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_43435</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711117.1">
        <property type="nucleotide sequence ID" value="XM_018857656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9C2"/>
    <dbReference type="GeneID" id="30030632"/>
    <dbReference type="OrthoDB" id="4023481at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008401">
        <property type="entry name" value="Apc13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05839">
        <property type="entry name" value="Apc13p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="169"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="211" mass="23876" checksum="FDABC0E49C0AF342" modified="2016-10-05" version="1">MSLDSQYSYVHFSHVNQADYMEDWKDYRIPYNDIDTTRLFEETANIFPQFLLEEEEDGAIPLSSSIFGAKVYKNRNLLKNKVWTDLNLDGLRTAPTLKRLPDASILNSISSSLVPADELALNEFLSNGYNADMSLNADALACNISHDELWLNTTSEYLGNHTFSHIKTRSNSGTHAAPLHQAPAPLQTPRVGKTRQPSQDKFQTPITRIMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q0</accession>
    <name>A0A1A0H9Q0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711364.1">
        <property type="nucleotide sequence ID" value="XM_018855870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Q0"/>
    <dbReference type="GeneID" id="30028846"/>
    <dbReference type="OrthoDB" id="160374at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052609">
        <property type="entry name" value="Ribosome_Biogenesis_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018849">
        <property type="entry name" value="Urb2/Npa2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15682:SF2">
        <property type="entry name" value="UNHEALTHY RIBOSOME BIOGENESIS PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10441">
        <property type="entry name" value="Urb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar 27S pre-rRNA processing Urb2/Npa2 C-terminal" evidence="1">
        <location>
            <begin position="937"/>
            <end position="1140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1140" mass="129241" checksum="42D78BD93FEFB167" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9R7</accession>
    <name>A0A1A0H9R7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711149.1">
        <property type="nucleotide sequence ID" value="XM_018858974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9R7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9R7"/>
    <dbReference type="GeneID" id="30031950"/>
    <dbReference type="OrthoDB" id="4153866at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF1">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="318" mass="36599" checksum="A0DAEC4A7CBE6C2F" modified="2016-10-05" version="1">MINVYRPVMANPPAAISVESLTRDVHLKPIHSHNDYWRKRPFLDALLYGCISIEADIWRFPKSYTVTDTTTESTAKFDRDDIYVGHNQVHLKPENTLRDLYLDPLYRFLESANNDHSEPILGAVSKKYGVFFDSPELTLNLWLDLKTNGSDTYLVLKSYLREFTEKQYLSHFNNSDLQRVEGPVAVTLTGDVPWALIELEDTIYDIRSVFLDCPLHQLVSASEQDRQRYGRLCLFASASLEQLIGTRDYEASLRHDFGEPQRAMLKEFFDAAHAVGLKARIWGGVDWPVHVRNMHWKSLWELGCDLINADDLESAAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W5</accession>
    <name>A0A1A0H9W5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamine-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8">6.3.5.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NAD(+) synthase [glutamine-hydrolyzing]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_44325</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>deamido-NAD(+) + L-glutamine + ATP + H2O = L-glutamate + AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24384"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.1" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711328.1">
        <property type="nucleotide sequence ID" value="XM_018857775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W5"/>
    <dbReference type="STRING" id="869754.A0A1A0H9W5"/>
    <dbReference type="GeneID" id="30030751"/>
    <dbReference type="OrthoDB" id="2020662at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00334"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07570">
        <property type="entry name" value="GAT_Gln-NAD-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000036">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.110.10:FF:000003">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02090">
        <property type="entry name" value="NadE_glutamine_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014445">
        <property type="entry name" value="Gln-dep_NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006630">
        <property type="entry name" value="NADS_GAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="Carbon-nitrogen hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="716" mass="79988" checksum="21116852F42DF9A8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA72</accession>
    <name>A0A1A0HA72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucosamine-6-phosphate isomerase</fullName>
            <ecNumber evidence="4">3.5.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Glucosamine-6-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79047</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-phosphate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:12172"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:75989"/>
            <dbReference type="EC" id="3.5.99.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.99.6" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711295.1">
        <property type="nucleotide sequence ID" value="XM_018858992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA72"/>
    <dbReference type="STRING" id="869754.A0A1A0HA72"/>
    <dbReference type="GeneID" id="30031968"/>
    <dbReference type="OrthoDB" id="7663298at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004342">
        <property type="term" value="F:glucosamine-6-phosphate deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006043">
        <property type="term" value="P:glucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006046">
        <property type="term" value="P:N-acetylglucosamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019262">
        <property type="term" value="P:N-acetylneuraminate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01399">
        <property type="entry name" value="GlcN6P_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004547">
        <property type="entry name" value="Glucosamine6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018321">
        <property type="entry name" value="Glucosamine6P_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00502">
        <property type="entry name" value="nagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11280:SF5">
        <property type="entry name" value="GLUCOSAMINE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01161">
        <property type="entry name" value="GLC_GALNAC_ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="5">
        <location>
            <begin position="14"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="252" mass="28325" checksum="DF123534FBB203B5" modified="2016-10-05" version="1">MRQAIFSSLQDASGFLARYIVDLINDFSPSADRPFVLGLPTGSSPEGIYSRLVEYYRSGRVSFKHVVTFNMDEYLGLYPTHPQLYLYFMYDKLFNHVDIQRENINILDGLAADPDQECLRYEEKIRLYGRIHFFLGGLGPEGHLAFNEAGSTRDSVTRRVDLVELTIAANARFFQNDPTKVPKQALSVGISTILDNTDEIAIIVLGAGKRYALEKTIHGRQNDPRFPSLFLSGHANVLLVCDQAAYGLTPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA77</accession>
    <name>A0A1A0HA77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Histone-fold-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_12718</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711300.1">
        <property type="nucleotide sequence ID" value="XM_018854260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA77"/>
    <dbReference type="STRING" id="869754.A0A1A0HA77"/>
    <dbReference type="GeneID" id="30027236"/>
    <dbReference type="OrthoDB" id="28335at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="6">
        <location>
            <begin position="82"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="233" mass="27166" checksum="A7ABA414F00B0078" modified="2016-10-05" version="1">MSDSESIHSDVSLDEEDEELIWRVFYSPLEEPAAAEEKPVDFDEDFSDFSDVEDQELVEKYRRLREQEEGEIEPDDDEKKRLLITSFSNDQMERFEAYRRMAVNKPGVKKICNSVLGHLIPQNIAVVMAGLSKLLLGDVITKAFEVQQNEFKTQLILDMEAKKKRKRETLQRLARGEDVLLMPEKNLEYLGDHKMPLTPEHIHEAWRLYRLENSGTFAAKWRTQGEGDGLMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA92</accession>
    <name>A0A1A0HA92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_44107</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711315.1">
        <property type="nucleotide sequence ID" value="XM_018857741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA92"/>
    <dbReference type="STRING" id="869754.A0A1A0HA92"/>
    <dbReference type="GeneID" id="30030717"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035361">
        <property type="term" value="C:Cul8-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990683">
        <property type="term" value="P:DNA double-strand break attachment to nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053036">
        <property type="entry name" value="CellCycle_DNARepair_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031906">
        <property type="entry name" value="RTT107_BRCT_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47667:SF1">
        <property type="entry name" value="REGULATOR OF TY1 TRANSPOSITION PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12738">
        <property type="entry name" value="PTCB-BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16771">
        <property type="entry name" value="RTT107_BRCT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="105"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="337"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="498"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="864" mass="94891" checksum="A7312589AC63B311" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAD4</accession>
    <name>A0A1A0HAD4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Formamidopyrimidine-DNA glycosylase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_41605</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.</text>
            <dbReference type="EC" id="3.2.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711347.1">
        <property type="nucleotide sequence ID" value="XM_018857434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAD4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAD4"/>
    <dbReference type="GeneID" id="30030410"/>
    <dbReference type="OrthoDB" id="444592at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008534">
        <property type="term" value="F:oxidized purine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.190.10">
        <property type="entry name" value="MutM-like, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012319">
        <property type="entry name" value="FPG_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035937">
        <property type="entry name" value="FPG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015886">
        <property type="entry name" value="H2TH_FPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22993:SF9">
        <property type="entry name" value="FORMAMIDOPYRIMIDINE-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01149">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06831">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00898">
        <property type="entry name" value="Fapy_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01232">
        <property type="entry name" value="H2TH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81624">
        <property type="entry name" value="N-terminal domain of MutM-like DNA repair proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51068">
        <property type="entry name" value="FPG_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Formamidopyrimidine-DNA glycosylase catalytic" evidence="11">
        <location>
            <begin position="2"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="206"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="378"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="434" mass="48919" checksum="950D646E0C2335D6" modified="2016-10-05" version="1">MPEVAEVAHACALLRRNVVGLTVRRAVLPLDNLLFPILKTAENPAAVIHQTENHLVGATVQSLGRHGKYFWLRFRTALGAPLVMLMHFGMTGRIRMRDVHSHMIFMENGGDRKVLEAKNSHIAGGDIEQEKDTEKAADSESLPEVWPPRFTKFELFFSGPSSAPGRSIDLAFLDPRRLARVRFLMGEEYSTDNKLLLMPPLRALGPDYSKQGTERSTLDGEPLPDSLADFSGDPDPNHHGRPRLSPEEFAPLVLLKKKPIKAFLLDQEFFAGIGNWVSDEILYHARIHPSEVISRKISSPEHPVLRRLYDAIVFVMEESVRVEGNARLFPEDWLMLHRWGKGRKEPRAVTTAGYTVDFLTVGGRTSCFIPELQKPLKTEDGSEMARDVKPKKDILLDKTPTPSPKSTRRSKAVEIAKIKEEENVGVKRRRRDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAM3</accession>
    <name>A0A1A0HAM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711438.1">
        <property type="nucleotide sequence ID" value="XM_018855882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAM3"/>
    <dbReference type="GeneID" id="30028858"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="278" mass="30897" checksum="20578CE3065F5534" modified="2016-10-05" version="1">MGAYATAWPCTVHQAVCSRAQSRAVSSTTWPCLLHHGRVFYNMAVYSMTFLSMTIYNSRVFGGMGAGDSSTLISSRIRFKILAPSRWQVQVRVVASLSPPESTVSGRAVSAPHMLGRPAGMLALADLWWVQMAGPLRKRAAVQKEPRLGHTFCPFCAFCPFRPFCPFCPFPLEALGWCARLLLPYYMRQHCSAHRRAPKKQATNSKRLVVRGPTLLGSEKMAFRKYQHIPGGFRKQPPALRTKIHLVLNTSSVIPAIRTSTLVCSSNIRPKLSPNPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAU7</accession>
    <name>A0A1A0HAU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711754.1">
        <property type="nucleotide sequence ID" value="XM_018854093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAU7"/>
    <dbReference type="GeneID" id="30027069"/>
    <dbReference type="OrthoDB" id="4089394at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033481">
        <property type="entry name" value="Dni1/Fig1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28092:SF1">
        <property type="entry name" value="FACTOR-INDUCED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12351">
        <property type="entry name" value="Fig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="20874" checksum="673FD09955CAF9DF" modified="2016-10-05" version="1">MKYNDTVIESAALSNSTSSVSELTIRANYLTLCASAAGDMVCTTSKNTTDLGTISSTDSSTDGVSLVVIASALSLVCTPYLLITALVIVLLLTIVVLWQLMPFVPGKVIARKICAAAALLASLVWGLGAMLQHQAIVSAEAFVEAATLNYISVERGSKAEALSWTAFLFLVITFFALLFRVFRDHRQLMALDEKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAW3</accession>
    <name>A0A1A0HAW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_78192</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711645.1">
        <property type="nucleotide sequence ID" value="XM_018858843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAW3"/>
    <dbReference type="GeneID" id="30031819"/>
    <dbReference type="OrthoDB" id="4076672at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="245" mass="27671" checksum="9D134EDA9545927A" modified="2016-10-05" version="1">MSLAARSALYFNYTICARLRLSPAPSCAKEVDFLLQNFRSIAPVDYFHVQQSSHSLSNPFRQIVTVVFNASEQPSQIDPFAPADDALDATPAQLEQRLNELLAYIRRICGIPRYSYIENDELYLEGRFMVPFQHKLLAEGQHLQGEYEISPSTSESPFFLLSGNHAAMDVLPHVRHNFQRLHKIEPLLVDSGRSALARLTGVAPDTQVQVNMNQVISTGALMDLDEELPWLAEKHNANGKFRGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB63</accession>
    <name>A0A1A0HB63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cell wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711761.1">
        <property type="nucleotide sequence ID" value="XM_018855925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB63"/>
    <dbReference type="STRING" id="869754.A0A1A0HB63"/>
    <dbReference type="GeneID" id="30028901"/>
    <dbReference type="OrthoDB" id="4096040at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291722" description="Cell wall protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="25036" checksum="E6FB0DDD814F16BA" modified="2016-10-05" version="1">MLAFTALLSLATYVFADITFSLTAKGDNINSNVGIDGSYLALNNQAATFILQTPSGYLTANGEDVVLTPKGIMLGSEDNRSKDFGIENGMLRSGDTNDMFDFYRCPDGTLANFHCTDGVAVTLYTGDVTESTSTAVEESTSTAAEESTSTVPTQETSSSSEEASWSSTQAWSSTEAATTSSVTQMPSYNSTVTDTHSTLVTITSCLDNACSLASASASTFEGGAAQAKYAAGAVALAGALLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB93</accession>
    <name>A0A1A0HB93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAM1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Outer kinetochore protein DAM1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_11827</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="15">Component of the DASH complex consisting of ASK1, DAD1, DAD2, DAD3, DAD4, DAM1, DUO1, HSK3, SPC19 and SPC34, with a stoichiometry of one copy of each subunit per complex. Multiple DASH complexes oligomerize to form a ring that encircles spindle microtubules and organizes the rod-like NDC80 complexes of the outer kinetochore. DASH complex oligomerization strengthens microtubule attachments. Within the complex, DAM1 and DUO1 may form the microtubule connections. On cytoplasmic microtubules, DASH complexes appear to form patches instead of rings. Interacts with the outer kinetochore component NDC80; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711791.1">
        <property type="nucleotide sequence ID" value="XM_018854106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB93"/>
    <dbReference type="STRING" id="869754.A0A1A0HB93"/>
    <dbReference type="GeneID" id="30027082"/>
    <dbReference type="OrthoDB" id="5586015at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990537">
        <property type="term" value="C:mitotic spindle polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013962">
        <property type="entry name" value="DASH_Dam1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28113:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08653">
        <property type="entry name" value="DASH_Dam1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="136"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="218"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="234"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA21281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="297" mass="32385" checksum="7215E6D46E367988" modified="2016-10-05" version="1">MHPHTPTGRLQALRRKLLRSSGVFRAIGPIAASPNVHYPKEIRPLEAPENRARLAAMADAFAALQQNMEGLAATHSAVNSFNESFASLFLGLQVHMFCNSFPGIPAREQYEAMEQKQSSEQKNAGLRAKVDAARRRNADLKRQASQQAAATRSRPHLGDLFSQRPLSRQRAAATAPKIPVARDDSFSATDTFIEAPGHSQPPPNLDQQPRYMRGLFEIPGAANTRRYDAARSGQKPSGKNTMNVPARARSRDAAAPPSTQKTASAAPHTSARQRASLAAQKAQKARAARMSSRPPFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBG5</accession>
    <name>A0A1A0HBG5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711738.1">
        <property type="nucleotide sequence ID" value="XM_018854089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBG5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBG5"/>
    <dbReference type="GeneID" id="30027065"/>
    <dbReference type="OrthoDB" id="4091299at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="625"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="636" mass="69227" checksum="E7C064877C3E5CA1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBU7</accession>
    <name>A0A1A0HBU7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711873.1">
        <property type="nucleotide sequence ID" value="XM_018854120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBU7"/>
    <dbReference type="GeneID" id="30027096"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013040186" evidence="1">
        <location>
            <begin position="16"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="114" mass="12698" checksum="881B5178EE6909D1" modified="2016-10-05" version="1">MILLLLFTLLATVLAIPTINNSEDHVRRCLGSTAQKYDISLKQEIFLAIKPKNAREFEFEGIDTRLVKCLGLVNPSDGVSTIITYNAIFARGKHKVSESALEKAVFEVIPDLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC77</accession>
    <name>A0A1A0HC77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32011</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712003.1">
        <property type="nucleotide sequence ID" value="XM_018855963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC77"/>
    <dbReference type="STRING" id="869754.A0A1A0HC77"/>
    <dbReference type="GeneID" id="30028939"/>
    <dbReference type="OrthoDB" id="996720at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002673">
        <property type="entry name" value="Ribosomal_eL29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12884:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01779">
        <property type="entry name" value="Ribosomal_L29e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="61" mass="7020" checksum="EF3950A77B58F752" modified="2016-10-05" version="1">MSKSKNHTNHNQNKKAHKNGIKKPKTYRYRTLKNVDAKFRRNHRYALQGTAKAVAEGRGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCC9</accession>
    <name>A0A1A0HCC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 6">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_32048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 6">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 6"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712047.1">
        <property type="nucleotide sequence ID" value="XM_018855972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCC9"/>
    <dbReference type="STRING" id="869754.A0A1A0HCC9"/>
    <dbReference type="GeneID" id="30028948"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF28">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleotide reductase large subunit" evidence="8">
        <location>
            <begin position="593"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="800"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="800"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="900" mass="100705" checksum="D975393BCC36FFC1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG9</accession>
    <name>A0A1A0HCG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_190779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712200.1">
        <property type="nucleotide sequence ID" value="XM_018854842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG9"/>
    <dbReference type="GeneID" id="30027818"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="140" mass="15992" checksum="CA3DC03025D6AF40" modified="2016-10-05" version="1">MHLEHWRKIQYYCLENSPCNCTFKSTKSAPTPLPCLPASSYCGSFVMLNLHSALLPWLLSPCTLYLLFVVSSIFLPFFDLFFLAFLVFFSQCNFQSHQSMARAFRLTTCKITIKLLMCRARVSGRGIFSNCRILPDAADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCJ3</accession>
    <name>A0A1A0HCJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41432</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NGG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712153.1">
        <property type="nucleotide sequence ID" value="XM_018857397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCJ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCJ3"/>
    <dbReference type="GeneID" id="30030373"/>
    <dbReference type="OrthoDB" id="1232at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019340">
        <property type="entry name" value="Histone_AcTrfase_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13556">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10198">
        <property type="entry name" value="Ada3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="78"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="564"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="571"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="588" mass="64660" checksum="93F1B038A2D614FD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS9</accession>
    <name>A0A1A0HCS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Mitochondrial escape protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_77378</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 12">Plays a role in maintaining the mitochondrial genome and in controlling the mtDNA escape. Involved in the regulation of mtDNA nucleotide structure and number. May have a dispensable role in early maturation of pre-rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the YME2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712307.1">
        <property type="nucleotide sequence ID" value="XM_018858685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS9"/>
    <dbReference type="STRING" id="869754.A0A1A0HCS9"/>
    <dbReference type="GeneID" id="30031661"/>
    <dbReference type="OrthoDB" id="10267654at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12433">
        <property type="entry name" value="RRM_Yme2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018850">
        <property type="entry name" value="Mt_escape_2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039627">
        <property type="entry name" value="Yme2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034260">
        <property type="entry name" value="Yme2_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32198:SF2">
        <property type="entry name" value="MITOCHONDRIAL ESCAPE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10443">
        <property type="entry name" value="RNA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4 12" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="156"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Mitochondrial escape protein 2 C-terminal" evidence="14">
        <location>
            <begin position="331"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF10443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="813" mass="91966" checksum="09B195BC3817D7C6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD13</accession>
    <name>A0A1A0HD13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein L35Ae</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30950</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712470.1">
        <property type="nucleotide sequence ID" value="XM_018855746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD13"/>
    <dbReference type="STRING" id="869754.A0A1A0HD13"/>
    <dbReference type="GeneID" id="30028722"/>
    <dbReference type="OrthoDB" id="1166329at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.190:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="18113" checksum="5ECAE8AF768F11B5" modified="2016-10-05" version="1" fragment="single">MALEYVGGSVLPPHHQTAPERACVFSAGCCFITAGVSGWARPVVVEIHGPYTHKIILDSTSQIPYTNIAVYVKGKHVSYQRSKSVTNPNVSLIQIEGVQTTKDAKFYIGKRIAYVYRAQKEVRGTKIRVIWGKVSRTHGNNGVVRANFKKNLPPKTFGASVRIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD26</accession>
    <name>A0A1A0HD26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_10716</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712283.1">
        <property type="nucleotide sequence ID" value="XM_018853832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD26"/>
    <dbReference type="STRING" id="869754.A0A1A0HD26"/>
    <dbReference type="GeneID" id="30026808"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508823" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="37317" checksum="19AB86CDF6B38674" modified="2016-10-05" version="1">MKLNTCVTTVVFYLLSLVFAVYIRSNTTKTEPNLLFDNLNIAERAYLSIVNCMDVDLGHYVLNEGDLYVTNTNGVSVRMIGQDFQNHGIISFNSFLSQGDHSTYHIAPKAYFSNSGEMFFGISSAPDDISPFIVTSEAGWRNQGMMVFRKEVVSTASLILGASGERPSTIVNDGSICLYNTFWQTSTSILGDGCIKVDHGSILDLNFAGGHSVAKNQTFLLESPGSVFRITGVDEDLPSVPVIKVGGLGDGNAIDFDFSLNATRSFVYSLEKVSIMGLSESPLIKIEIGPGYVLESLVLSTGPIGSRLSYELPVPHGHVHVCRCQSEFPDIPETPIVPLSSCMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD41</accession>
    <name>A0A1A0HD41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">tRNA (uracil-O(2)-)-methyltransferase</fullName>
            <ecNumber evidence="3 11">2.1.1.211</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_67056</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-methyltransferase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>uridine(44) in tRNA(Ser) + S-adenosyl-L-methionine = 2'-O-methyluridine(44) in tRNA(Ser) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10339"/>
            <dbReference type="Rhea" id="RHEA-COMP:10340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.211"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the TRM44 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.211" evidence="3 11"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712427.1">
        <property type="nucleotide sequence ID" value="XM_018858363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD41"/>
    <dbReference type="STRING" id="869754.A0A1A0HD41"/>
    <dbReference type="GeneID" id="30031339"/>
    <dbReference type="OrthoDB" id="10047021at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141101">
        <property type="term" value="F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011671">
        <property type="entry name" value="tRNA_uracil_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21210:SF0">
        <property type="entry name" value="TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07757">
        <property type="entry name" value="AdoMet_MTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 11" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="545" mass="61668" checksum="2F0F75D313EA7BB5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD51</accession>
    <name>A0A1A0HD51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_144839</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712505.1">
        <property type="nucleotide sequence ID" value="XM_018854508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD51"/>
    <dbReference type="STRING" id="869754.A0A1A0HD51"/>
    <dbReference type="GeneID" id="30027484"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="619" mass="70013" checksum="FCAA4207F60B9B8B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDG0</accession>
    <name>A0A1A0HDG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712428.1">
        <property type="nucleotide sequence ID" value="XM_018857293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDG0"/>
    <dbReference type="STRING" id="869754.A0A1A0HDG0"/>
    <dbReference type="GeneID" id="30030269"/>
    <dbReference type="OrthoDB" id="1933281at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF3">
        <property type="entry name" value="GH10162P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="1">
        <location>
            <begin position="207"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="537" mass="58366" checksum="BC8BF13C2298C627" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDL1</accession>
    <name>A0A1A0HDL1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein PXR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">PinX1-related protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein pxr1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_229172</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINX1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712509.1">
        <property type="nucleotide sequence ID" value="XM_018855221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDL1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDL1"/>
    <dbReference type="GeneID" id="30028197"/>
    <dbReference type="OrthoDB" id="29523at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010521">
        <property type="term" value="F:telomerase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032211">
        <property type="term" value="P:negative regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF31">
        <property type="entry name" value="PROTEIN PXR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="G-patch" evidence="10">
        <location>
            <begin position="25"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="150"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="184"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="290" mass="33261" checksum="E83568AFB6DE5B5C" modified="2016-10-05" version="1">MGLAGVKVKQRFGLDPRNTSWSNNTERFGHQYLEKMGWAPGKGLGLVDHATTSHVKVSIKTDNSGLGSKLAKKTKKDEFDSGECAGLDVFQRILGRLNGKEEQVSSELDRKRTDNIVNGKWGMHFIKGETLSSTWDSESKKLLLYSSKKRLSPEDDEPAGRKSKKSKSASLRDERKEKNEKKEKKEKKEKKEKKEKKEKKEKKEKKQKKEKKGKKEKKDKKEKKKDLRLEAQLSGNEQATEVTRESMLQPREGNEKQIATRLSARAKWIRQKRASVMDAKALNEIFMITN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDM3</accession>
    <name>A0A1A0HDM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein LOT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_39751</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LOT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712612.1">
        <property type="nucleotide sequence ID" value="XM_018857171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDM3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDM3"/>
    <dbReference type="GeneID" id="30030147"/>
    <dbReference type="OrthoDB" id="19714at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039924">
        <property type="entry name" value="ICln/Lot5/Saf5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03517">
        <property type="entry name" value="Voldacs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="209"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="277" mass="29831" checksum="D30584A6168A79FC" modified="2016-10-05" version="1">MGKSPRFIHECPNVENTLPLELFRAQSPARYSQGDGDKMVLYAGNGDCSLEILATSSIHVHEKLRAAGSSVAVFVLDTSVVLWFLGMQGSTGLGVELPYPLIALHALKDVEGESVLYLQVLSCELFQSVAVSEYTQTVELILRAGRPGQQTSLLFYSPLLVAPCAVPQLYEALGTCSALHYDSEPESETDGFGADHEWITADHEWITADSGPGPQLEMPASWVNSGDADDLGMADPSEENSDGEAGMNVAVGYVTVAGVVRRRSSVSREAQKQRRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDM5</accession>
    <name>A0A1A0HDM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal protein bL31m N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_86820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712680.1">
        <property type="nucleotide sequence ID" value="XM_018859267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDM5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDM5"/>
    <dbReference type="GeneID" id="30032242"/>
    <dbReference type="OrthoDB" id="5587740at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034600">
        <property type="entry name" value="Ribosomal_bL31m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048874">
        <property type="entry name" value="Ribosomal_bL31m_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28174">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L36, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28174:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL31M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21492">
        <property type="entry name" value="bL31_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein bL31m N-terminal" evidence="1">
        <location>
            <begin position="35"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="158" mass="17991" checksum="64EE2788C1341EDB" modified="2016-10-05" version="1">MFCSSRSTLQAGVQIQFLRNVSRKIAMVNDVHLSNRRVMKKIKMGAARPTIFHQFDTLVEFSDGSVIKRRSQAPKDEIRLISDQKNNALWNPNRSDLSNGDLSTTGRVGLFMKKFAQFQEEPAENKEQQTDGLVELLGNNVTEVQKGGRLREKRRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDV6</accession>
    <name>A0A1A0HDV6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zn-dependent exopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_145993</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712573.1">
        <property type="nucleotide sequence ID" value="XM_018854519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDV6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDV6"/>
    <dbReference type="GeneID" id="30027495"/>
    <dbReference type="OrthoDB" id="3064516at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="6">
        <location>
            <begin position="210"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="503" mass="55348" checksum="8EBAA8DFB28DC949" modified="2016-10-05" version="1">MSIMYDAGFRNQSAAKLAGAVRIQTHVTDSAPSVDQDPAYWHEKFAPFHDYLQATFPELWRFCQVEKVNSWGLVITWPGSDAGLRPLLLGAHQDVVPIQEASLDQWTYPPFEGVYDGTRLWGRGSADCKNLLIGLLEAAEALHAEGFRPRRTVVFAFGFDEEIGGDNGARNIGLFLLARYGPGGFYAVFDEGGQSLVKMGDVALALIGTSQKGSLNYVVSLNTPGGHSSVPPDHTAIGIVSELVTRLENAPFLPMFTARNPTFAQYQCLAAHSAEMEPGLKRAIRHLDSCPRARQLVAEKIYNSDLTSRYLVTTSQAVDIIHGGVKSNALPEYVEVLVNHRIAVESSVQEVLDRNMAIVGAVADRYGLGIDAGGSEIVPRTQNGHFSISYMDLLEPAPATPVYDEHWALLAGTLRHVYEEVCEGTMAEFDGTPILPVPGMAPGNTDSKHYWDLSKHIYRYRPGLVPNVQAHAHGADEHIVFDAHLQIIAFYYEYIQLADWARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE07</accession>
    <name>A0A1A0HE07_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712830.1">
        <property type="nucleotide sequence ID" value="XM_018855821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE07"/>
    <dbReference type="GeneID" id="30028797"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="11228" checksum="4376623FEBA4503F" modified="2016-10-05" version="1">MDLAGSLPDAEEDPHMGSKKVGTTVEMSDTLVSNITDDGWKVQGRKRRNSRKRPERSVIMATTTGSILTDQKGSLGSTPSMKNTSSNLNPFLVLAEISEEDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HED7</accession>
    <name>A0A1A0HED7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_151865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712846.1">
        <property type="nucleotide sequence ID" value="XM_018854573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HED7"/>
    <dbReference type="GeneID" id="30027549"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="94" mass="10335" checksum="7DDBF3D6323868ED" modified="2016-10-05" version="1">MYLVNGPHGGPSNGPYNGPHAGPYNPSIKDLILVLIKVPPSDFHDLYTKDHEISPIQTPTETIMWLAWDIVLIAMPSYTDQINHSAQAVAINTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEE7</accession>
    <name>A0A1A0HEE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Protein ARG5,6, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_40172</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2 18">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">In the C-terminal section; belongs to the NAGSA dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">In the N-terminal section; belongs to the acetylglutamate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712856.1">
        <property type="nucleotide sequence ID" value="XM_018857241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEE7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEE7"/>
    <dbReference type="GeneID" id="30030217"/>
    <dbReference type="OrthoDB" id="438291at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04252">
        <property type="entry name" value="AAK_NAGK-fArgBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23936">
        <property type="entry name" value="AGPR_C_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd24149">
        <property type="entry name" value="AGPR_N_ARG5_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04263">
        <property type="entry name" value="DUF619-NAGK-FABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000019">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000029">
        <property type="entry name" value="Bifunctional acetylglutamate kinase/N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000011">
        <property type="entry name" value="N-acetyl-gamma-glutamyl-phosphate reductase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041734">
        <property type="entry name" value="NAGK-fArgBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011241">
        <property type="entry name" value="NAGK/NAGSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036440">
        <property type="entry name" value="ARG5-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 18" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="15 18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16 18" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12 18" id="KW-0521">NADP</keyword>
    <keyword evidence="9 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="20">
        <location>
            <begin position="304"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00072977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR036440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA22360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="837" mass="91113" checksum="93CB4F82A409F65C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEM3</accession>
    <name>A0A1A0HEM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_153647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018854591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEM3"/>
    <dbReference type="GeneID" id="30027567"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="75" mass="8900" checksum="7611714B55CFC8FA" modified="2016-10-05" version="1">MGIAPGERSAGLARIPGHVPLLALEEDTTKRRTKRERKRRRNRETKKNRTKHQKQKQKQNKTKKKNTKDRQNTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEN6</accession>
    <name>A0A1A0HEN6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712931.1">
        <property type="nucleotide sequence ID" value="XM_018855829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEN6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEN6"/>
    <dbReference type="GeneID" id="30028805"/>
    <dbReference type="OrthoDB" id="4096987at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="32562" checksum="F11C7A4DD7000D3A" modified="2016-10-05" version="1">MLELETKDTVLKETVQTVNDHQDPVYAESREREDSKNEGRQREAPKNEGREREDSKNEDRQREDSKNDDIEREDSKNDDREKDAALTSASLKAVKKDQQQDEVVLGRKRAFEINDNEALQSIQESHAAQDGVSKLTEKSLVRSLELHVDHANRTRESINHLSFLDVQSSTPLKTQKEASSATKHFLPRISMDSALAEIKDLDDAIDYITQMAAQGHELHNDVDGALTNFISAMPEEEEEMTVEEWIRHNAASCRKIVEDLGEQIVRNYEAEFDKVINYVAGLATID</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HET9</accession>
    <name>A0A1A0HET9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA polymerase V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_31380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712986.1">
        <property type="nucleotide sequence ID" value="XM_018855840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HET9"/>
    <dbReference type="STRING" id="869754.A0A1A0HET9"/>
    <dbReference type="GeneID" id="30028816"/>
    <dbReference type="OrthoDB" id="342531at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007015">
        <property type="entry name" value="DNA_pol_V/MYBBP1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213:SF2">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13213">
        <property type="entry name" value="MYB-BINDING PROTEIN 1A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04931">
        <property type="entry name" value="DNA_pol_phi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="744"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="751"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="771"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1054" mass="117069" checksum="BF67DE04725E2082" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEW3</accession>
    <name>A0A1A0HEW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NDT80 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_11280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712944.1">
        <property type="nucleotide sequence ID" value="XM_018853952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEW3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEW3"/>
    <dbReference type="GeneID" id="30026928"/>
    <dbReference type="OrthoDB" id="4117572at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1390">
        <property type="entry name" value="NDT80 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052605">
        <property type="entry name" value="Fungal_trans_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024061">
        <property type="entry name" value="NDT80_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037141">
        <property type="entry name" value="NDT80_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144">
        <property type="entry name" value="MEIOSIS-SPECIFIC TRANSCRIPTION FACTOR NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35144:SF1">
        <property type="entry name" value="PROTEIN PACG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05224">
        <property type="entry name" value="NDT80_PhoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51517">
        <property type="entry name" value="NDT80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NDT80" evidence="4">
        <location>
            <begin position="325"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="NDT80" evidence="2">
        <location>
            <begin position="325"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="303"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="623"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="736"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="654"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="676"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="736"/>
            <end position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="771" mass="84730" checksum="3E88F262FC60E5E6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEY8</accession>
    <name>A0A1A0HEY8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Utp12-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_39798</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat WDR3/UTP12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713036.1">
        <property type="nucleotide sequence ID" value="XM_018857179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEY8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEY8"/>
    <dbReference type="GeneID" id="30030155"/>
    <dbReference type="OrthoDB" id="407922at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000157">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000178">
        <property type="entry name" value="WD repeat domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25173">
        <property type="entry name" value="Beta-prop_WDR3_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25172">
        <property type="entry name" value="Beta-prop_WDR3_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="405"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="499"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="598"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="640"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="682"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="7">
        <location>
            <begin position="830"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="971" mass="104785" checksum="A119C08ED60ABDCF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEZ3</accession>
    <name>A0A1A0HEZ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uracil-DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_19941</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712958.1">
        <property type="nucleotide sequence ID" value="XM_018854911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEZ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEZ3"/>
    <dbReference type="GeneID" id="30027887"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.470.10:FF:000007">
        <property type="entry name" value="Uracil-DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003589">
        <property type="entry name" value="PRK05254.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="8">
        <location>
            <begin position="79"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="32177" checksum="51E433CC6C2CD014" modified="2016-10-05" version="1" fragment="single">EYVNFCSRHNFDKNEWIAGLTAEERGLLQLEIEHLHVTWLALLHKELIKPYFICLKQFLHSQKGKTIFPPPKDIYSWSHYTPLPEVKCLVLGQDPYHNFNQAHGLAFLVLEPTRPPPSLLNIYKALKLDFPDFVAPDYKKLLKEGKPGGGNLTKWAKRGVLMLNACLTVEAHKANSHANRGWEQFTEKVIETAITYARENKKQGLVIMAWGTPAQKRVAKFSLQLSKKEVPFLVLKSVHPSPLSASRGFFDLHVFEKCNRWLKEQGREPINWGLVEMNNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF33</accession>
    <name>A0A1A0HF33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycerophosphocholine acyltransferase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_39455</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713107.1">
        <property type="nucleotide sequence ID" value="XM_018857144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF33"/>
    <dbReference type="GeneID" id="30030120"/>
    <dbReference type="OrthoDB" id="406287at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106158">
        <property type="term" value="F:glycero-3-phosphocholine acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036151">
        <property type="term" value="P:phosphatidylcholine acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090640">
        <property type="term" value="P:phosphatidylcholine biosynthesis from sn-glycero-3-phosphocholine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021261">
        <property type="entry name" value="GPCAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31201">
        <property type="entry name" value="OS01G0585100 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10998">
        <property type="entry name" value="DUF2838"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="428"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="437"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="53380" checksum="B588594B6B5639EE" modified="2016-10-05" version="1">MAEGLEAPSNAKTGEEIAEFPVDALESDDEALVHSVPMRRSDSSVSVSFLDLLKLLVMPDYDFGSGFDQVSDSTKRKFMEMKNRTREKLLRFKADKQVAGLRSKTKKFSPQDLAKFQEGLNKRLNRFDSAIQESLQSLATEKAFYAFAVTLIGAAGFIIGKYPVYFPVFHTVLFCVLMPIRFYTYFKLGFQYYLADLCYYVNVLLLLFLWVFPHSESLFVSVFLLALGTLSFAVITWRNSLVLHLIEKTTSSFIHVMPPLTMFILVHQTPADYVARRYPAVALVGKWNFVNGIIVTSIYYTVWQVAYHYFITVKRREQIAKGRVTSFTYLKKSQSKSPLGRFVNSLPYPWMQIAAFTLIQFGYQMLTMLPCPIWFRYKYACGAFVLFVFTWSAYNGATYYIEVFGKRFEKELKQLRIEIEELQLRLDRQEGERDSAKTSPDVSPVIQATSGEPLVVNQGDRDAEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF53</accession>
    <name>A0A1A0HF53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription initiation factor TFIID subunit 1 histone acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_164164</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713127.1">
        <property type="nucleotide sequence ID" value="XM_018854639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF53"/>
    <dbReference type="STRING" id="869754.A0A1A0HF53"/>
    <dbReference type="GeneID" id="30027615"/>
    <dbReference type="OrthoDB" id="5752at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040240">
        <property type="entry name" value="TAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022591">
        <property type="entry name" value="TAF1_HAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041670">
        <property type="entry name" value="Znf-CCHC_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12157">
        <property type="entry name" value="DUF3591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15288">
        <property type="entry name" value="zf-CCHC_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 1 histone acetyltransferase" evidence="7">
        <location>
            <begin position="425"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc knuckle" evidence="8">
        <location>
            <begin position="1072"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="776"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1115"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1000"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="167"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="787"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1146" mass="128478" checksum="88D159CE0153D0D8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF82</accession>
    <name>A0A1A0HF82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Redoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_29250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713137.1">
        <property type="nucleotide sequence ID" value="XM_018855370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF82"/>
    <dbReference type="STRING" id="869754.A0A1A0HF82"/>
    <dbReference type="GeneID" id="30028346"/>
    <dbReference type="OrthoDB" id="195498at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PEROXIREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430:SF16">
        <property type="entry name" value="PEROXIREDOXIN-5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Redoxin" evidence="7">
        <location>
            <begin position="48"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="6">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR637944-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="191" mass="20705" checksum="83A1D9022712CF5A" modified="2016-10-05" version="1">MTLSSGSSFPTGVELTYIPIDLATIKQANALGCAKPFTLKVDRLIEQNADSNILFVSVPVAFAPTCTEGHSQPFLAHLEKLKKEKDISVLIILSANDPFVMNAWGKLLLQNADFNPDSELPKVIFASDPNAEFSVANGMALDLTNEGLGIRTGRYAFVVRTGTRSVVYYGMDPGHDDVVASSYETIETAQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFH7</accession>
    <name>A0A1A0HFH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713221.1">
        <property type="nucleotide sequence ID" value="XM_018855383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFH7"/>
    <dbReference type="GeneID" id="30028359"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013040235" evidence="1">
        <location>
            <begin position="16"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="138" mass="15426" checksum="8AC4561DED8C3158" modified="2016-10-05" version="1">MKLFTALSFVALVLAAPRRVTVGRLAIRDVNSDPDIPDEYFVVLDENAIFLVQESPFNFKFLEGYVTAEVGKEQKYMGINEKNKLALVENKMGLKIVGGFLKMGDESVFQLCSDNTLALGSDCDVKQIIQIKFVPDSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFV2</accession>
    <name>A0A1A0HFV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cell division control 14, SIN component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713362.1">
        <property type="nucleotide sequence ID" value="XM_018855406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFV2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFV2"/>
    <dbReference type="GeneID" id="30028382"/>
    <dbReference type="OrthoDB" id="5357220at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012535">
        <property type="entry name" value="Cell_div_Cdc14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34065">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34065:SF1">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08045">
        <property type="entry name" value="CDC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="251" mass="28551" checksum="3828C043F5CFDCB7" modified="2016-10-05" version="1">MEKDLEGILDHLNSRIHQKISRGLDSLENFLKSFVPSIRKYHRSSFLDNKLASLIALQNNFQYNLASGFMLVYSTFSKSSEKIDDYVLIKTNYLLSGILLIHPESRALFGRGQSMALMLSFLNSKSSAILEDLSISFVSLFIHILMKNLKNVRLFEANNGCQLLIHHLSILPANSCETSTKESDTSRQRNLHFKVIEFLIFYLADETELLDLPQNVGAHSMSVREKADLLRPQFPGIDDLIKNLSDLTSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG77</accession>
    <name>A0A1A0HG77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF803-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_9232</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713363.1">
        <property type="nucleotide sequence ID" value="XM_018859339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG77"/>
    <dbReference type="GeneID" id="30032314"/>
    <dbReference type="OrthoDB" id="2504919at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF86">
        <property type="entry name" value="ADR321CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="46175" checksum="AA34AFC59687D1EB" modified="2016-10-05" version="1">MIGSSVMWGCVAAVMLSAIQSLGITLQRKSHIIPYDHELSENSVVNLRRDSSVSIDYESTHKTHNYRRNIWLLGFSMFIFANIFGSLIQLTALPLIILSPLQSIGLIFNSIFSCTLLPGEKFTKKLAGGTVIIALGAFFIAFNGNSIAQPPLDIGADEKFQRVLDKFARPGFLTWWSLTFIVMAILVRVNWLISVKIKFLQSKPHRRTRRLSRTSKDPLSRLVFTRGILYGLVSGTLTAHTFLFAKSIVDVIFEMILERVTPKCVSTYVIAVFLLIATLCIVVMQLAAFNLGLSNILTAILYPLCFLVYNLVNLFNDIFFNTLFSTGGMDISQFIYVLIGLSGVLYGVVLISWDSACGKPEVSSDDTQPLSVTKFPYEHKEGENRVFSYEESEIISQFSYINHDPNLDGFVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG86</accession>
    <name>A0A1A0HG86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioesterase/thiol ester dehydrase-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_34921</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713373.1">
        <property type="nucleotide sequence ID" value="XM_018856538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG86"/>
    <dbReference type="STRING" id="869754.A0A1A0HG86"/>
    <dbReference type="GeneID" id="30029514"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="3">
        <location>
            <begin position="30"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="4">
        <location>
            <begin position="215"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="38825" checksum="616E603FF5CCC40B" modified="2016-10-05" version="1">MSQFQDIFSVRKTGKNTYIANHPLTKAHPSAKGVYGGNIVGQAVLVAIKSAPKGFTPHSVHSYFAKIVSEKASIEWEVDEISNGNNFCGRCIRAVQKGETKFTATISLARKNSLQVAELAYQQYEEKERLKIENRKIARANGKLQESEEVEEEDDEPVIDKPFQFQVPYPEWLKEVNIDDLLVDRRTSLRMIYHKIPTKLVTLKGSEEEDNIPATDRRVAWFVRLGLGDVEITDSAYQFSALAVLSDSMFLTRIARVLRIPDIDLSDYAHYFSVSLDHTIFFHDTDFDATEWMGFAFKAIRYSNGRFLLEAQMYNSAKQHVATVFQEGLAHFNGIEREAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGH0</accession>
    <name>A0A1A0HGH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">W2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76307</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713745.1">
        <property type="nucleotide sequence ID" value="XM_018858509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGH0"/>
    <dbReference type="GeneID" id="30031485"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11561">
        <property type="entry name" value="W2_eIF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000031">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.350:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003307">
        <property type="entry name" value="W2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02020">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00515">
        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51363">
        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="W2" evidence="6">
        <location>
            <begin position="235"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="397" mass="44008" checksum="E378734C6DC84720" modified="2016-10-05" version="1">MSFINICRDNTDPFYRYKMPPIQAKVEGRGNGIKTAIPNLSEVARALGRPSPYVVKFFGYEMGAQTTFKEDDDRYLVNGSHMQSELQDCLDGFINKFVLCGSCKNPETQIQIKGKGNLQKDCKACGSITEVDPRHKLSSYILKNPPPASTGKKAATATANVVGGGKTISEIAQSAEGGDAQGQGDDDDDILTKKINAEAAALSRQTVEVADEDWAVDMSKEAIEARARELEGISLSDSQTKVSEFGEWVLEQASDSKDDLPSDVEIYKKIVELELLESPEVLLVLAQVLFDEDIVEQLEAHQGLLVKLINEREDYERAFLGGLERFLGLEKTELIPAVPKILHRLYDSEIISEEAIIEWGSKVSKKYVPKDVSKKVRKAGKPFVKWLQEADEESDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGJ9</accession>
    <name>A0A1A0HGJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">LuxS/MPP-like metallohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_76114</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713459.1">
        <property type="nucleotide sequence ID" value="XM_018858479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGJ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGJ9"/>
    <dbReference type="GeneID" id="30031455"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007323">
        <property type="term" value="P:peptide pheromone maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000004">
        <property type="entry name" value="Putative insulin-degrading enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="8">
        <location>
            <begin position="36"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="9">
        <location>
            <begin position="200"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="10">
        <location>
            <begin position="386"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="11">
        <location>
            <begin position="788"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1033"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1039"/>
            <end position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1069" mass="122316" checksum="112FA641663004C0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGN9</accession>
    <name>A0A1A0HGN9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713499.1">
        <property type="nucleotide sequence ID" value="XM_018855438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGN9"/>
    <dbReference type="GeneID" id="30028414"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="69" mass="7669" checksum="6B34E4B4073CE801" modified="2016-10-05" version="1">MIKSTEALAKFSFDSYSPPRQAEKDTSFSVQYNHNCDACLIHLKASKASAHSKENEAARETPIVILIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS8</accession>
    <name>A0A1A0HGS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_29597</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLM10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713539.1">
        <property type="nucleotide sequence ID" value="XM_018855444.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HGS8"/>
    <dbReference type="GeneID" id="30028420"/>
    <dbReference type="OrthoDB" id="17907at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032430">
        <property type="entry name" value="Blm10_mid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032372">
        <property type="entry name" value="Blm10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035309">
        <property type="entry name" value="PSME4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021843">
        <property type="entry name" value="PSME4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32170:SF3">
        <property type="entry name" value="PROTEASOME ACTIVATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16547">
        <property type="entry name" value="BLM10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16507">
        <property type="entry name" value="HEAT_PSME4_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11919">
        <property type="entry name" value="PSME4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Proteasome activator Blm10 N-terminal" evidence="8">
        <location>
            <begin position="74"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator Blm10 middle HEAT repeats region" evidence="7">
        <location>
            <begin position="518"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="Proteasome activator complex subunit 4 C-terminal" evidence="6">
        <location>
            <begin position="2110"/>
            <end position="2196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="211"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2196" mass="250153" checksum="AA4A0F55A5297000" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH16</accession>
    <name>A0A1A0HH16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein L16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_103633</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713772.1">
        <property type="nucleotide sequence ID" value="XM_018853751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH16"/>
    <dbReference type="STRING" id="869754.A0A1A0HH16"/>
    <dbReference type="GeneID" id="30026727"/>
    <dbReference type="OrthoDB" id="268521at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000010">
        <property type="entry name" value="60S ribosomal protein L16, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_uL16_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_uL16_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220:SF13">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL16M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00701">
        <property type="entry name" value="RIBOSOMAL_L16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="228" mass="25716" checksum="DF95BDD2245F0DAE" modified="2016-10-05" version="1">MFGMRLVSSWKAAQPFSRPLTPLPAVQSIFGTITKRYKHEYAPRFKQIRKAFKGRVTVRTGGSIKGNSLEFGLYGLRLKSNGIRMTATQLKEADKVIQREIRPSGATCVTRFACNIPVCVKGNQTRMGKGKGSFDHWACRVPTGKVLFEIKGDVHDRVAREAMRKAADKLPGLFEIIDANSKVRVSTTDLIDKPSAVNYVELIKQKPTKKWTNIEMSKLPEFRLFRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH24</accession>
    <name>A0A1A0HH24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Actin-like ATPase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_36180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713658.1">
        <property type="nucleotide sequence ID" value="XM_018856703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH24"/>
    <dbReference type="STRING" id="869754.A0A1A0HH24"/>
    <dbReference type="GeneID" id="30029679"/>
    <dbReference type="OrthoDB" id="29851at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0101031">
        <property type="term" value="C:protein folding chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006452">
        <property type="term" value="P:translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="469"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="476"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="549" mass="58724" checksum="1873D5FBEA901C8E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHD1</accession>
    <name>A0A1A0HHD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphomethylpyrimidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_102756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713729.1">
        <property type="nucleotide sequence ID" value="XM_018853727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHD1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHD1"/>
    <dbReference type="GeneID" id="30026703"/>
    <dbReference type="OrthoDB" id="10028886at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19367">
        <property type="entry name" value="TenA_C_ScTHI20-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004305">
        <property type="entry name" value="Thiaminase-2/PQQC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03070">
        <property type="entry name" value="TENA_THI-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="Heme oxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="2">
        <location>
            <begin position="29"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Thiaminase-2/PQQC" evidence="1">
        <location>
            <begin position="348"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="577" mass="63854" checksum="6CCB2274ED56868A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHK7</accession>
    <name>A0A1A0HHK7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_9602</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713809.1">
        <property type="nucleotide sequence ID" value="XM_018859416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHK7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHK7"/>
    <dbReference type="GeneID" id="30032391"/>
    <dbReference type="OrthoDB" id="61900at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="73"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16630" checksum="3058760B455870E3" modified="2016-10-05" version="1">MPTRFTKTRKHRGNVSAGKGRVGKHRKNPGGRGMAGGQHHHRTNLDKYHPGYFGKVGMRYFHKQRNHFWKPQINVERLWSLVEEEKRDQYLKSASASAAPVIDTLAHGYGIVLGKGSLPNVPVIVKARFVSKLAEEKIRAAGGVVELIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHU5</accession>
    <name>A0A1A0HHU5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldehyde dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_9715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713933.1">
        <property type="nucleotide sequence ID" value="XM_018859446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHU5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHU5"/>
    <dbReference type="GeneID" id="30032421"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019413">
        <property type="term" value="P:acetate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006067">
        <property type="term" value="P:ethanol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07091">
        <property type="entry name" value="ALDH_F1-2_Ald2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000001">
        <property type="entry name" value="Mitochondrial aldehyde dehydrogenase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000007">
        <property type="entry name" value="NAD/NADP-dependent betaine aldehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="35"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="499" mass="53861" checksum="2137EA3747C2E1A5" modified="2016-10-05" version="1">MARLYSNLPLSVPITLPNGLKYEQPTGLFINNEFVHPAQKKSFAVISPSTEEEVAHVYEALEEDVDSAVQAAQAAFDGGWSAADPSVRAKALNKIADIIEENAELFASIEAFDNGKSISNARGDVALAVGCFRSTAGWADKITGSVVETGESHFNFTRREPLGVCGQIIPWNFPLLMFTWKVGPALATNNTIVLKTSETTPLSALYLCKLLQEQQVLPAGVLNVVNGFGRTTGNAITEHPMIKKVAFTGSTATGKLIMKKCSSTLKKVTLELGGKSPHIIFDDANIDVAVQNVITGIFYNSGEVCCAGSRLYIQEGIYEEFVKKFVAAAEAVKVGNPFDEEVMQGAQNSQNQLDKILGYIKSGKEEGATLLSGGERSEGKGFFVKPTIFGDVTESMKIVTEEIFGPVITVHKFKTTDEVVKMANDSEYGLAAGIQSTNVNTCIDVSRRIRAGTIWVNTYNDFHPMVPFGGFGSSGIGREMGSAVLDNYTQVKAVRMALH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHU8</accession>
    <name>A0A1A0HHU8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pumilio homology domain family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29891</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PUF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713899.1">
        <property type="nucleotide sequence ID" value="XM_018855505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHU8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHU8"/>
    <dbReference type="GeneID" id="30028481"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032473">
        <property type="term" value="C:cytoplasmic side of mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051646">
        <property type="term" value="P:mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010795">
        <property type="term" value="P:regulation of ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000004">
        <property type="entry name" value="Pumilio homolog 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUM-HD" evidence="9">
        <location>
            <begin position="437"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="458"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="494"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="530"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="566"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="602"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="638"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="674"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="7">
        <location>
            <begin position="721"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="297"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="372"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="310"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="402"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00060736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00081811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="800" mass="88720" checksum="8E9612931A77FEB5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI08</accession>
    <name>A0A1A0HI08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mog1p/PsbP-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_76523</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MOG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713998.1">
        <property type="nucleotide sequence ID" value="XM_018858543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI08"/>
    <dbReference type="STRING" id="869754.A0A1A0HI08"/>
    <dbReference type="GeneID" id="30031519"/>
    <dbReference type="OrthoDB" id="10255285at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007681">
        <property type="entry name" value="Mog1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016123">
        <property type="entry name" value="Mog1/PsbP_a/b/a-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15837:SF0">
        <property type="entry name" value="RAN GUANINE NUCLEOTIDE RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04603">
        <property type="entry name" value="Mog1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55724">
        <property type="entry name" value="Mog1p/PsbP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="187" mass="20505" checksum="5C3C7487A82F19CA" modified="2016-10-05" version="1">MGFAKVSLYGGAISSMFPEGAIDVSDIREVPDTQEVFLLEQANKLDQALIVDLLETVEAPTLPEIISVHLGDILDEAPLYLAPLETFFNEDVQAHVHTFLVKPAPSKQETETTKLFMYITLLQVVKAQSDILITMTVPHESGEVSQDAFFQMAQNAVSGTEPVLGLAYNVVKTAASTFKICEWGLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI52</accession>
    <name>A0A1A0HI52_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SYM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_10059</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714325.1">
        <property type="nucleotide sequence ID" value="XM_018853676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI52"/>
    <dbReference type="STRING" id="869754.A0A1A0HI52"/>
    <dbReference type="GeneID" id="30026652"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="229" mass="26339" checksum="EF675B7049666C76" modified="2016-10-05" version="1">MTGPFKFYNCLLKSHPYKTNMIGSGVFFGAGDVLAQYLFPHHIDDNFENPTEYHLDRTYRAMVYGCFFFGPVSVKWHTKTLPYIMSPFLSAAKRAKMKPYSMHIHDTLFRLTLDALLMPGLIWIPMYNTVMSALAFHLDPLAVASEKLRNNWWGVLKANWTVWTPLQLFNLFCVPIHLRIVVSNFWSIGWNCFLSFVHNTSGHGKGSGKLIEELVDIESADEEQTMVYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIJ0</accession>
    <name>A0A1A0HIJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714311.1">
        <property type="nucleotide sequence ID" value="XM_018855576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIJ0"/>
    <dbReference type="GeneID" id="30028552"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="56" mass="5991" checksum="FE53441B4FFD42F6" modified="2016-10-05" version="1">MGPSISRAWGAGGLVISALWRNEALAADRTGLAGLLGMAFWVWLFCMSLLGMAFFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIJ7</accession>
    <name>A0A1A0HIJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_35699</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peroxisome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxin-22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714307.1">
        <property type="nucleotide sequence ID" value="XM_018856646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIJ7"/>
    <dbReference type="GeneID" id="30029622"/>
    <dbReference type="OrthoDB" id="4014227at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11730">
        <property type="entry name" value="Peroxisome assembly protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024359">
        <property type="entry name" value="Peroxin-22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038613">
        <property type="entry name" value="Peroxin-22_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12827">
        <property type="entry name" value="Peroxin-22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="187" mass="20853" checksum="498213F24ECE020E" modified="2016-10-05" version="1">MARKKSPRMLAAILVASSVAAISYKLYGVLKGQSDSKSPLDAKDGGVALSRRRQYRNKSITVTLLHSVLNLGLPLNEMLLSSENVTFILPPYLSVDDLACQIESSENGALYELPLTLIRNYKLLKCSNIDGYFHLLKDLRPDVLFVCTDDLGIDRVPPDLRRFVSEIVAIEQDKENVRSVLSDVFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIN1</accession>
    <name>A0A1A0HIN1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_116093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714495.1">
        <property type="nucleotide sequence ID" value="XM_018854033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIN1"/>
    <dbReference type="GeneID" id="30027009"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="588"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="687" mass="73118" checksum="D5C94315EBD4C7F3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIW2</accession>
    <name>A0A1A0HIW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_25918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714575.1">
        <property type="nucleotide sequence ID" value="XM_018855305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIW2"/>
    <dbReference type="GeneID" id="30028281"/>
    <dbReference type="OrthoDB" id="10512009at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="81" mass="8784" checksum="DF07681E55D0CA75" modified="2016-10-05" version="1" fragment="single">SVTDLRNSLASNVAFSVRPLRKSTKSPASEIDSTGVTSLITFSNANISSPFFFSVLYASRSSGYFSSYFTNFFNFARTTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIY0</accession>
    <name>A0A1A0HIY0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_30457</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714595.1">
        <property type="nucleotide sequence ID" value="XM_018855641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIY0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIY0"/>
    <dbReference type="GeneID" id="30028617"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291817" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="37209" checksum="8137810C6A98C537" modified="2016-10-05" version="1">MRLNICVTLPYFFTQILAVIISQNTITTNQRVEVIASLTINEGVFYSIIHNNFLTLLDKFENAGRLYVTASTGSQASLLLTGIHFKNSGVIVFESFPLGESTYHIYAENFFENNGVMLFGTLGESSGITRISVLARESWTNTGMMFFVESRGLPSHLLLGKSNSTRKNVTITNEGTICFKNLFWRSFTRIEGYGCIAIGFESTFEVDISKYSVSPLQIFSLDPTNSRLIVRGLKTPMDEIPVIKVVGLGEGNSIEVEVLYRQIAAWEFSSPGLFSLLIADTPRVTFDLGPEYSLRDFQVSASFYGCKITVHRPVPPLPLVCRCDVEFPSAPTALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ56</accession>
    <name>A0A1A0HJ56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30249</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714354.1">
        <property type="nucleotide sequence ID" value="XM_018855586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ56"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ56"/>
    <dbReference type="GeneID" id="30028562"/>
    <dbReference type="OrthoDB" id="413079at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000286">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000308">
        <property type="entry name" value="MFS efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051788">
        <property type="entry name" value="MFS_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514">
        <property type="entry name" value="BYPASS OF STOP CODON PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23514:SF6">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="410"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="96"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="540" mass="59259" checksum="63F02E087D978F91" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD1</accession>
    <name>A0A1A0HJD1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_117883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714587.1">
        <property type="nucleotide sequence ID" value="XM_018854090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJD1"/>
    <dbReference type="GeneID" id="30027066"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291854" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="78" mass="8561" checksum="41664F3C15EE3CE7" modified="2016-10-05" version="1">MSITLSVWLLLLPHEISTICNWPRPPERAVYISAVGPSVVTRTRYLLPVVAPSIASRTRCLLLVAGPSIAPRTRCLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF3</accession>
    <name>A0A1A0HJF3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37182</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714770.1">
        <property type="nucleotide sequence ID" value="XM_018856836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJF3"/>
    <dbReference type="GeneID" id="30029812"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="24"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="71"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="112"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="288"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="321" mass="34442" checksum="C326301514E217AC" modified="2016-10-05" version="1">MSLITNPVKNAESRSILFEKAVVLSGHTGAVFSIEFSADGHNIASAGMDRTIRLWNLPTTADNEAPNYGVIEGHKKAITSLSWILSSKILSASADSTVSVWDAETGQRLKTGKGHELTVNDCSTSSEGPYLSVGDDGTIRLWDEREKYEVSKIETPYPILACDLSSDGKIAYVAGIDPSVRAYDVSSGSLLWSCDGFTETTTGLALSSDESMLVLRAMDGSVYTINAKSFVPAGVSRKGQTYEGAVGGAQMLVSRAKFSHDDIYICLASDDHLATMWGTASQRMAAKLTGHEAAVVDIDFHPREKILASCALDGDIIVREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK46</accession>
    <name>A0A1A0HK46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="4">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">tRNA wybutosine-synthesizing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_30788</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; wybutosine-tRNA(Phe) biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715035.1">
        <property type="nucleotide sequence ID" value="XM_018855711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK46"/>
    <dbReference type="STRING" id="869754.A0A1A0HK46"/>
    <dbReference type="GeneID" id="30028687"/>
    <dbReference type="OrthoDB" id="271553at2759"/>
    <dbReference type="UniPathway" id="UPA00375"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000196">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="16">
        <location>
            <begin position="116"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="17">
        <location>
            <begin position="361"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA24554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="714" mass="80764" checksum="1E2F2FDD5EBCAC0D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK89</accession>
    <name>A0A1A0HK89_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_81530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714699.1">
        <property type="nucleotide sequence ID" value="XM_018859212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK89"/>
    <dbReference type="GeneID" id="30032188"/>
    <dbReference type="OrthoDB" id="10429073at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="110" mass="12093" checksum="339304743766FE42" modified="2016-10-05" version="1">MLEKPHELPLSFFDAEPPSHYCCQLCDFHCQWGHIVDYNMVVGAVSPNLLLLNLGLIAYALVFRTHAEPCAGPSHGHVRRILWARLGHLLLGLAILAVLVAGAVVWVMAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKA9</accession>
    <name>A0A1A0HKA9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_36560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714803.1">
        <property type="nucleotide sequence ID" value="XM_018856758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKA9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKA9"/>
    <dbReference type="GeneID" id="30029734"/>
    <dbReference type="OrthoDB" id="440760at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039771">
        <property type="entry name" value="Csl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048626">
        <property type="entry name" value="CSL4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019495">
        <property type="entry name" value="EXOSC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE CSL4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12686:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT CSL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21551">
        <property type="entry name" value="CSL4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10447">
        <property type="entry name" value="EXOSC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exosome complex component CSL4 N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Exosome complex component CSL4 C-terminal" evidence="5">
        <location>
            <begin position="128"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="303" mass="32505" checksum="D88BE5BB7346C585" modified="2016-10-05" version="1">MTTVVPGQYLVPEYKTEAGESGDVVLRYFPGKGAVLSSIDSGSRSIPVIVATVLGEQVIQEIKKNELETPDHETNDDKEEESISKKEDSNIRTFLVSVVLGKKSEYTQYAKEIDASASDSVSATATNLPREGDEVLVRITRLNLKQAFCEVLCVYGYGNVSPDGGLGSNGTSAHFSVPMGGGSQILSSYGAVASSQSFMSGAQPLDIGEAFKAVIRSQDVRSTDRDKVKIIDCFRPGDLVKAVVLSLGDGSNYYLTTARNDLGVIFAKSEGGAGDLMIAVDWETMVCEKTGVVEKRKCAKLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HL37</accession>
    <name>A0A1A0HL37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6PF2K-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_30767</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715004.1">
        <property type="nucleotide sequence ID" value="XM_018855700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HL37"/>
    <dbReference type="STRING" id="869754.A0A1A0HL37"/>
    <dbReference type="GeneID" id="30028676"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF32">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="4">
        <location>
            <begin position="384"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="218"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="150"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="267"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="974" mass="108476" checksum="C0CFF3D77A1A6F91" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYW1</accession>
    <name>A0A1A0GYW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_17791</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709258.1">
        <property type="nucleotide sequence ID" value="XM_018854735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYW1"/>
    <dbReference type="STRING" id="869754.A0A1A0GYW1"/>
    <dbReference type="GeneID" id="30027711"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46156">
        <property type="entry name" value="CCCH ZINGC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46156:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="62"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA16959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="221" mass="25099" checksum="8A06EC0D6FF716FD" modified="2016-10-05" version="1" fragment="single">NATETDACDRVRIGTDVFGVSSNGTRLLPISGKKRQEGSVVVWNHWRYLVCKSGLLRRTFGEPPRDYCRYFTCNGSCVRGVQCRFIHDMSRMALCRHYLAGRCLGHCILSHEASEYNTPVCRYHLEDRCSNARCNFSHELPAHARDSDFSIWTCRPFAVGGWCDRGRLCPFAHLYNCPDYEESGLCPRGNSCPLTHTITKRSFEPQNTIVSSYSVDPAVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ27</accession>
    <name>A0A1A0GZ27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_14059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000012">
        <property type="protein sequence ID" value="OBA16945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709247.1">
        <property type="nucleotide sequence ID" value="XM_018854475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ27"/>
    <dbReference type="GeneID" id="30027451"/>
    <dbReference type="OrthoDB" id="4085737at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA16945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="739" mass="85362" checksum="A177363A6B039DAA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ47</accession>
    <name>A0A1A0GZ47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_33869</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709264.1">
        <property type="nucleotide sequence ID" value="XM_018856411.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ47"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ47"/>
    <dbReference type="GeneID" id="30029387"/>
    <dbReference type="OrthoDB" id="269151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01856">
        <property type="entry name" value="YlqF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45782:SF4">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="3">
        <location>
            <begin position="127"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA16965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="368" mass="41555" checksum="8DD0BF8448C17702" modified="2016-10-05" version="1">MTFVPRTSFPDYRLTLASFKGHHQKALTKFGHLAPQIDLILEVRDSRAPVSTTNVLFDRVLARKQKIILYSKKDLSIMKPALLRKWHQNEPFLFVDTRNPKDARRILDAVGAFYSQMKPPPPLGLRAMIIGMPNVGKSTLVNTLRSVGYSSQLPTGISSKKNKKVARTGGQPGVTRNTSEIIRLSRDPDLLVYDTPGVFLPTVKDPETMLALSLVGCIQESFVDPVIQADYLLYVLNLQSKNGEGYAEFMDRPTNDIHELLHNIAKKKGRVSRDGSWDQLGMAQHWTNTWRQGKLRKFKGLFDVPAILEVSSTEMKQTIESELERLKDSGVHTRIINSLGEDGTGNSKARNRTAKDREFDIRNQLFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Y9</accession>
    <name>A0A1A0H1Y9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_80129</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RPAP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709441.1">
        <property type="nucleotide sequence ID" value="XM_018859175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Y9"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Y9"/>
    <dbReference type="GeneID" id="30032151"/>
    <dbReference type="OrthoDB" id="348201at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039913">
        <property type="entry name" value="RPAP1/Rba50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013929">
        <property type="entry name" value="RPAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013930">
        <property type="entry name" value="RPAP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21483:SF18">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08620">
        <property type="entry name" value="RPAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08621">
        <property type="entry name" value="RPAP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RPAP1 N-terminal" evidence="4">
        <location>
            <begin position="78"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="RPAP1 C-terminal" evidence="3">
        <location>
            <begin position="260"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA18046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="426" mass="47380" checksum="BF2DDE29B9B9B0C3" modified="2016-10-05" version="1">MDLIGDIVEHEASAPSNPVVDQHAAASGFPQLRKFEGLKPRNSRFRSGGPRLSPAESKMHAGARHSAPKDEESLTEAQRIHRENLEKISALTEDELTHEREELLQGLDPRLVQTLLRRTEARVADHDHDHAEGYGGWIGSGKNGASLPALNDQDVNRALGIKSVSFREDVEDKEHAEGPDEDAEVAPEGYQIVPETQPEVHFPKPRPAREDPDLDLDDPAFFSRLHEKYFPDLPKETSKLAWMTTPMPTHAVASYEAIGDMRFDFKGNLVQLDQKARDTPTHLGLHHHAENPQLAGYTLGELVHLSRSVVPTQRCLGIQMLGRVLHKLGLHKYNVMPVVGPGDAASGFEAAAHELMGQFEDMMWDLVESLRVVESLTEASDETRTRNLSVRSYAIEALWLWKQGGGRPPAAQKSEVDALAENLHRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Z8</accession>
    <name>A0A1A0H1Z8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">5-demethoxyubiquinone hydroxylase, mitochondrial</fullName>
            <shortName evidence="8">DMQ hydroxylase</shortName>
            <ecNumber evidence="8">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ubiquinone biosynthesis monooxygenase COQ7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">COQ7</name>
        <name evidence="9" type="ORF">METBIDRAFT_13960</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the hydroxylation of 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) during ubiquinone biosynthesis. Has also a structural role in the COQ enzyme complex, stabilizing other COQ polypeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + AH2 + O2 = a 3-demethylubiquinol + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="8">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion inner membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
            <orientation evidence="8">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the COQ7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709451.1">
        <property type="nucleotide sequence ID" value="XM_018854462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Z8"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Z8"/>
    <dbReference type="GeneID" id="30027438"/>
    <dbReference type="OrthoDB" id="275371at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160224">
        <property type="term" value="F:3-demethoxyubiquinone 3-hydroxylase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237:SF4">
        <property type="entry name" value="5-DEMETHOXYUBIQUINONE HYDROXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="COENZYME Q10 BIOSYNTHESIS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 8" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA18056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="203" mass="23035" checksum="9A5F9CD83116E867" modified="2016-10-05" version="1">MSKAQKAFLDRVIRVDQAGELGANYIYMGQYGVLSLKYPHLKPILKHMWEQEIYHHDNFNRLQTERRVRPSLFTPLWKIGAVAMGAGTALISKEAAMACTVAVETVIGGHYNQQLRVLMKQYNIDAFDKKTGELLLPEQLTEAARTSPELSKLKDMISTFRDDELEHLDTAVENDAEKAVPYILLTETIKLVCRGAIWTAERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4S3</accession>
    <name>A0A1A0H4S3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33589</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9 10">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709485.1">
        <property type="nucleotide sequence ID" value="XM_018856342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4S3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4S3"/>
    <dbReference type="GeneID" id="30029318"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.480:FF:000002">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000007">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 9 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="11">
        <location>
            <begin position="46"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA18950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="322" mass="36079" checksum="CB9DCA8DB4371674" modified="2016-10-05" version="1">MPKAPKKKFSTTASPAGATVSAEKHMNSVFKFNTNLGQHILKNPLVAQGIVDKAQIKPSDVVLEVGPGTGNLTVRILEQAKKVIAVEMDPRMAAELTKRVHGTPQQKKLDILLGDFMKTELPYFDVCISNTPYQISSPLVFKLLNQPKPPRVSILMFQREFALRLLARPGDSLYCRLSANVQMWANVTHIMKVGKNNFRPPPQVESSVVRIEIKQPRPLVDFEEWDGLLRIVFVRKNKTIAAGFKSNNVIEILTKNYTTYLAQQDADSMQVDSKGDLTAVVKQKIESVLTETGYSDQRAAKMDQTDFLKLLYAFHQVGIHFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H517</accession>
    <name>A0A1A0H517_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_47494</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709553.1">
        <property type="nucleotide sequence ID" value="XM_018858174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H517"/>
    <dbReference type="STRING" id="869754.A0A1A0H517"/>
    <dbReference type="GeneID" id="30031150"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03339">
        <property type="entry name" value="TCP1_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000003">
        <property type="entry name" value="T-complex protein 1 subunit epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="544" mass="59232" checksum="B42CEE06C50DAED6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H539</accession>
    <name>A0A1A0H539_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79996</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709573.1">
        <property type="nucleotide sequence ID" value="XM_018859156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H539"/>
    <dbReference type="STRING" id="869754.A0A1A0H539"/>
    <dbReference type="GeneID" id="30032132"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051299">
        <property type="entry name" value="AB_hydrolase_lip/est"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640:SF1">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="5">
        <location>
            <begin position="81"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="34961" checksum="829579E5580657B3" modified="2016-10-05" version="1">MKVYENLGTYAALIDISYCIDTYHQIEEPFQCDLCGSFESNMSLVSQWYFDDSVCGYISTTFWNIFDYDDSGNSVNRKTIIVSLRGTRSLHDTITDLKVDLVKYENIGQNLLLCGPDCRVHEGFWRYYKSTLEVMESKLKSEIEMSFLNYEIIFIGHSLGGSVALLLAIHFLDMGFSNLTLVTMGQPLIGNKEFTTWADEVLGSSFPVAHNSYARKYIRVIRKGDIVTTVPRSGLLFERYCSFENQIYINATADTVIPSPEEVVDCFSGENPTCIARDFALPFAPLTDFYHNHNTYFRHMGLCGIHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5I2</accession>
    <name>A0A1A0H5I2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">lactoylglutathione lyase</fullName>
            <ecNumber evidence="4">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Aldoketomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketone-aldehyde mutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Methylglyoxalase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">S-D-lactoylglutathione methylglyoxal lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_33372</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glyoxalase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709694.1">
        <property type="nucleotide sequence ID" value="XM_018856276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5I2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5I2"/>
    <dbReference type="GeneID" id="30029252"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="12">
        <location>
            <begin position="9"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="12">
        <location>
            <begin position="160"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="326" mass="37067" checksum="9E2B9FC5ED14BCD2" modified="2016-10-05" version="1">MAASDKSLLMNHTCLRVKDPKVSVPFYEKHFGMTLINHFIMDGFTLYMMGYKSATPKNWSARPGVLELCHNHGAENDATYKLNNGNGDKFRGFGHTCITVDNIEACEKQLLADGVQFKKKLSDGRQQNIAFVLDPDGYWIELVEHKNNHQAGATDKATYRFNHTMVRVKDPEKSLHFYREVLGMKLFSRKDFESAQFSLYFLGYDHDPSFKEGELGYLDQLGREGVVELTHNWGTENDDAFEGYHNGNSTENGALQGYGHICVSSATPGQFCKDIENKFGDSLNWSVKWNQGKMKDLAFIRDPDGYSIEIIGHDIFHDWVDGRPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5I5</accession>
    <name>A0A1A0H5I5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NCA2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_47058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709710.1">
        <property type="nucleotide sequence ID" value="XM_018858119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5I5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5I5"/>
    <dbReference type="GeneID" id="30031095"/>
    <dbReference type="OrthoDB" id="413313at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013946">
        <property type="entry name" value="NCA2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28234:SF1">
        <property type="entry name" value="NUCLEAR CONTROL OF ATPASE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08637">
        <property type="entry name" value="NCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="482" mass="52658" checksum="D7CD9C0E0FEB3B00" modified="2016-10-05" version="1">MDEYCVLLGLHHLANSILYKTIVAKTHLAYWAQVKLSLWGKLVYGVQTLPLRAWRVIGAGFCGAVAAAKSGPGPTWASCGVGPLVRALRRVLASTWRRVNADFVVRQSRLLFLKIPMKGLDAEIRGHTAALAAELDEHHRMLGVFVNSLPVSRGTLAQIVPQSGVPAGGAVGGQLDFIAQFVAGESRASLAAPPGAVARYWPLLLVAAQYGPALSLNAWQNRAAIAAWVRRTFVDTAAGFWRNWVVKPVGDMLSILRNDEAMALALKQSLRADLDSLERMVLAYVADTHVDVAPADVRAAVRLGDLTMVMARYEDELRTPYRALLGGLLVRALLIQVQKTKVDGDLAITGIDKLLKLQQLLLGVLSVLPSLFILYQAQRALFKDAALTQSFVDRRVDCLKSLNLISRLLNREQAADKYVADGKLFVEVVNLSLLAHQIIPAALRDEFVGDLNLLALACSDDSIDVRAAAGRVWNMYAPYFRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5R1</accession>
    <name>A0A1A0H5R1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import inner membrane translocase subunit TIM14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM18</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_79884</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, it is required to stimulate activity of mtHSP70 (SSC1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer with PAM16. Component of the PAM complex, at least composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TIM14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709903.1">
        <property type="nucleotide sequence ID" value="XM_018859143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5R1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5R1"/>
    <dbReference type="GeneID" id="30032119"/>
    <dbReference type="OrthoDB" id="240298at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF28">
        <property type="entry name" value="GEO10507P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="15">
        <location>
            <begin position="92"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="16422" checksum="449ADF15A9D3625A" modified="2016-10-05" version="1">MEVPTLPIPGETPSAQQQAFTGHLQKKKAAEGTAEWYFDKTSDWMGEHPWMTGLGVLGVVYFASGFVKSKQPGINGKAFVRGSFGAKMSAKEALQILNLKESTLSKAKLKEQHRKLMMANHPDKGGSSFLATKVNEAKDFLEKRGGLKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5W5</accession>
    <name>A0A1A0H5W5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">PIN domain-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_33493</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. GEN subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709840.1">
        <property type="nucleotide sequence ID" value="XM_018856308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5W5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5W5"/>
    <dbReference type="GeneID" id="30029284"/>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048256">
        <property type="term" value="F:flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09904">
        <property type="entry name" value="H3TH_XPG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09868">
        <property type="entry name" value="PIN_XPG_RAD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000030">
        <property type="entry name" value="Flap endonuclease GEN-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000061">
        <property type="entry name" value="Single-stranded DNA endonuclease (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001044">
        <property type="entry name" value="XPG/Rad2_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-G CELLS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16171:SF7">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00066">
        <property type="entry name" value="XRODRMPGMNTG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00842">
        <property type="entry name" value="XPG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="15">
        <location>
            <begin position="793"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="119"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="495"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="641"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="721"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA19308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1043" mass="118595" checksum="01C09C742B009E2A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5X5</accession>
    <name>A0A1A0H5X5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="2">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_46471</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709850.1">
        <property type="nucleotide sequence ID" value="XM_018858044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5X5"/>
    <dbReference type="STRING" id="869754.A0A1A0H5X5"/>
    <dbReference type="GeneID" id="30031020"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140836">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat S5 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905866">
        <property type="term" value="P:positive regulation of Atg1/ULK1 kinase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07841">
        <property type="entry name" value="STKc_CDK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000097">
        <property type="entry name" value="Putative cyclin-dependent kinase 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037770">
        <property type="entry name" value="CDK7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="9"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="15"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR637770-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="320" mass="36330" checksum="1D12B9691DD7AB1D" modified="2016-10-05" version="1">MPATTAQTYTKDQKVGEGTYAVVYVGKQVKTNRKIATKEIKTGLFKDGLDMSAIREVKYLKELQHPNVIELIDVFMSHDNNLNLVLEFLPCDLEVLIKDQSIVFKSSDIKAWLLMTLRGLHHCHRNFILHRDLKPNNLLLAPDGQLKIADFGLARAFGNANEDFTPKVVTRWYRAPELLFGAKHYTGAVDIWAVGIIFAELMLRTPYLPGKDDLDQLDVTFKALGTPTEQVWPNVSNLPLYNALRVYPLPSRQELRNRFSAATEKALDLMISMTQLDPSRRCDLTAALLSEYFTEMPSATPPLELPTKKSEKDEKRRKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Y2</accession>
    <name>A0A1A0H5Y2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_26468</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709969.1">
        <property type="nucleotide sequence ID" value="XM_018855316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Y2"/>
    <dbReference type="GeneID" id="30028292"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="11"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="112" mass="12189" checksum="1A55B53B724EF4E5" modified="2016-10-05" version="1" fragment="single">LTLDNHGAVARDHMANERTFLAWLRTSLSLVTIGVGTVQLLKLESESQNLSRFAVPLGASFIMVGMLTLVMGSFRYFRVQSMLLSSLYPASQFLVSVLAAAVFLLVVVTLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6H4</accession>
    <name>A0A1A0H6H4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc-finger protein ZPR1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_45835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a protein folding chaperone for elongation factor 1-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZPR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710212.1">
        <property type="nucleotide sequence ID" value="XM_018857965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6H4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6H4"/>
    <dbReference type="GeneID" id="30030941"/>
    <dbReference type="OrthoDB" id="308464at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.420:FF:000002">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000001">
        <property type="entry name" value="Zinc finger protein ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000003">
        <property type="entry name" value="Zinc finger protein zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="Zn_ribbon_ZPR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="10">
        <location>
            <begin position="45"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="10">
        <location>
            <begin position="289"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="242"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="482" mass="53794" checksum="34EB9B1E2C3E3952" modified="2016-10-05" version="1">MPDTPAQQQQENFSSVGDQAQGIDSSINDTGASDAQGHPVQEVESLCMNCHKNGTTRMLLTKIPFFREIIIMSFECPHCGLKNSEIQPAAQIAEKGSRYIFKIENKEDFNRQIVKSETATCMFTELELEIPPERGQLTNIEGLLLEMVLNLSADQPARKEAQPEVYEKLAAFIERVQACLNGDAGCLPLTFYVDDPAGNSWVEFTPGQPSHKWSMYEYNRTAEQNVFLGLISADDVAQHRKLESEQKKAATSTNVSSTLNNDNPKSSGFTVHDHNDIENLSSEVQTFHATCSSCFEPCETNMKSVDIPHFKEVIIMSTFCEHCGYKSNEVKTGGAIPDKGRKISLKITDPDDLARDILKSETCGLKIPELNLDLTPGTLGGRFTTIEGLLSQVYDELHSRVFTQTSDSMEEETKQRWTSFFAKLQDASEGKIGFTVVMEDPLAASYVQNVYAPDNDPNMTIEEYDRTEEQNEDLGLNDMKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6H6</accession>
    <name>A0A1A0H6H6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_19233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710093.1">
        <property type="nucleotide sequence ID" value="XM_018854849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6H6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6H6"/>
    <dbReference type="GeneID" id="30027825"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF1">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="28874" checksum="BE9BB284788F7A3E" modified="2016-10-05" version="1" fragment="single">PFWNWKTIVGFGAAGAYLAYSEWIFDAYARATEVDPSGLLPIQLEYRLKTLPVYQQLANTSAGDDWVMMKSYENLDRNALDNSAASGAYVRLGNTAAAAVKSEAAYAAQHLTSHTLAQPGGIAVPPVIFHNRSTNDTVTIVHMGYRLCGYPFIVHGGMLATLLNETFKRNASLRHDAAGFLKDDYKVESLAISYRRPAFADQFFVLRTEQVPAPGPDRPAVYKSTIEAENGAVLVEAHATLRHTGRASHRHSQEQASRWALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6K2</accession>
    <name>A0A1A0H6K2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SWIB-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_218087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710189.1">
        <property type="nucleotide sequence ID" value="XM_018855109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6K2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6K2"/>
    <dbReference type="GeneID" id="30028085"/>
    <dbReference type="OrthoDB" id="10251073at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd10567">
        <property type="entry name" value="SWIB-MDM2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014876">
        <property type="entry name" value="DEK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019835">
        <property type="entry name" value="SWIB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08766">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00151">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109715">
        <property type="entry name" value="DEK C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51998">
        <property type="entry name" value="DEK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEK-C" evidence="3">
        <location>
            <begin position="4"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="120"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="255" mass="29200" checksum="3B0267AB9FAF1B83" modified="2016-10-05" version="1">MTQTFDASDYQEMIDAILSAADLDKISIKRIRNAILELFDVDLMPHKEKVNELILNRYYKLADERANPQPNQEDLENQDHLMALRLQKEGQKHDTRRGRSAVSKVSKPRKTGARTAPNNGFNADLLLSPQLQEVIGSARMSRPQVVKQMWVYIRAHDLQNASDKRQILCDEKLTKIFKKNTVTMFEMNKLLSKHLYKEDELTSNCSLSIAGLIENEEKKPPKLSEENSRSLSPDTTVKREKQACDDASEISEVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Z2</accession>
    <name>A0A1A0H6Z2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45193</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710250.1">
        <property type="nucleotide sequence ID" value="XM_018857890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Z2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Z2"/>
    <dbReference type="GeneID" id="30030866"/>
    <dbReference type="OrthoDB" id="5352132at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00065">
        <property type="entry name" value="FYVE_like_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="6">
        <location>
            <begin position="295"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="76"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="50904" checksum="E510916D5493B449" modified="2016-10-05" version="1">MTEPSDPAQDADLPPFSFSKRPIISTMATSAIPHNIDRALLNVLKPADVPTFNNVNYDDRIRAPRMSLATHYTYKPELPRPESSEHNVDGSSQAGPTTPGCGNEDAQLEYRPATADLNQPTRRNLEPIKQKWGILVSTGNILSLDTVEYPEIYGTAAKFRSGSGALPGTTPLMQQKLVRAIENLDANTTLEERAAHAERANSFCKPASGRNRVYMPSSGPAIQQVYTQKKPLCLPAVLRPPQEIDKINEEDFLHDLSADEGSFEAKLGEFPFEMPQDATLQNSRAEPTHEHWAPNSSSDHCMNCFEVFRGFFNPQRRGRHHCRFCGLLYCHNCLYKSREAFQIAPPAADTQTQLRANSGSSNSSSKFKLCKVCKTCGNNSLRLLYLLNQRSRAKNDIDTPYVFIDNPYIGASHALQAHPLYDLKSRATRPGFKNEGGSERRASYNHVPSDWTWSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H760</accession>
    <name>A0A1A0H760_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_45390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710454.1">
        <property type="nucleotide sequence ID" value="XM_018857912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H760"/>
    <dbReference type="GeneID" id="30030888"/>
    <dbReference type="OrthoDB" id="4017494at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="55" mass="6361" checksum="D3670FCB2118963E" modified="2016-10-05" version="1">MNLWQKWISLPVKMRYYIGGSTAVFALIGDYATAQINEEITNRKKILNEIEEGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7A2</accession>
    <name>A0A1A0H7A2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710499.1">
        <property type="nucleotide sequence ID" value="XM_018856180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7A2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7A2"/>
    <dbReference type="GeneID" id="30029156"/>
    <dbReference type="OrthoDB" id="194139at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="358"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="611" mass="70768" checksum="6E98E20AF4623406" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7B5</accession>
    <name>A0A1A0H7B5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">P-type Ca(2+) transporter</fullName>
            <ecNumber evidence="2">7.2.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_12904</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.10" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710395.1">
        <property type="nucleotide sequence ID" value="XM_018854277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7B5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7B5"/>
    <dbReference type="GeneID" id="30027253"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005388">
        <property type="term" value="F:P-type calcium transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000002">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000028">
        <property type="entry name" value="Calcium-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000193">
        <property type="entry name" value="Plasma membrane calcium-transporting ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093:SF369">
        <property type="entry name" value="CALCIUM-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24093">
        <property type="entry name" value="CATION TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="366"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="857"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="884"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="927"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1022"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1054"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1079"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="44"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1105"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA19870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1138" mass="124556" checksum="B733A386BA25F8B6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7B7</accession>
    <name>A0A1A0H7B7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphoadenylyl-sulfate reductase (thioredoxin)</fullName>
            <ecNumber evidence="3">1.8.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3'-phosphoadenylylsulfate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">PAPS reductase, thioredoxin dependent</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">PAdoPS reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_45285</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[thioredoxin]-disulfide + sulfite + adenosine 3',5'-bisphosphate + 2 H(+) = [thioredoxin]-dithiol + 3'-phosphoadenylyl sulfate</text>
            <dbReference type="Rhea" id="RHEA:11724"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="EC" id="1.8.4.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAPS reductase family. CysH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.8" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710514.1">
        <property type="nucleotide sequence ID" value="XM_018857903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7B7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7B7"/>
    <dbReference type="GeneID" id="30030879"/>
    <dbReference type="OrthoDB" id="7869097at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004604">
        <property type="term" value="F:phosphoadenylyl-sulfate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019379">
        <property type="term" value="P:sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23945">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000151">
        <property type="entry name" value="Phosphoadenosine phosphosulfate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00063">
        <property type="entry name" value="CysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004511">
        <property type="entry name" value="PAPS/APS_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011800">
        <property type="entry name" value="PAPS_reductase_CysH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00434">
        <property type="entry name" value="cysH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02057">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002537">
        <property type="entry name" value="PRK02090.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46509">
        <property type="entry name" value="PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46509:SF1">
        <property type="entry name" value="PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000857">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="37"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="27739" checksum="6B7CF9E039E90B3F" modified="2016-10-05" version="1">MISLTQDHIDHLNKTLVKLSAPEILTWAAITFPGLHQTTAFGLTGLVILDMISKTNPVDLIFIDTLHHFPQTYDLVEKVKLKYNPNLHVFKPQGVTTDLEFTEKHGDSLWETNDSLYDFLVKVEPAQRAYAELGVQAVLTGRRKSQGGARAALPVIEVEKSSGIIKINPLWDWDFGQVKKYIDENNVPYNELLDMGYKSIGDWHSTEPVAEGEDERSGRWKGKAKTECGIHQTSRFAQYMEKGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7C8</accession>
    <name>A0A1A0H7C8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thioredoxin-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nuclear thiol peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_79159</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peroxiredoxin family. BCP/PrxQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710327.1">
        <property type="nucleotide sequence ID" value="XM_018859011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7C8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7C8"/>
    <dbReference type="GeneID" id="30031987"/>
    <dbReference type="OrthoDB" id="338622at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03017">
        <property type="entry name" value="PRX_BCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000157">
        <property type="entry name" value="DOT5p Nuclear thiol peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050924">
        <property type="entry name" value="Peroxiredoxin_BCP/PrxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801:SF23">
        <property type="entry name" value="PEROXIREDOXIN DOT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42801">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="15">
        <location>
            <begin position="35"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="22601" checksum="EC3C4B2ED553C22A" modified="2016-10-05" version="1">MPELRRSARASTVEATKRLGQPPSKKAKTENPKELEVGDEIPNLTLLNENEDEISLKEVAEKSKYVVIFAYPKASTPGCTKQVTGFQRNYSDLQALGAAVYGLSADLPKSQLNFVTKQGLKYPLLSDPSRKFISILGAKKSPSGIKRSHWIFVDGKLSIKKIQISPDVSVISALEDVRKLKKDSESGDAKSEVADEAKAENVDPRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7I0</accession>
    <name>A0A1A0H7I0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710383.1">
        <property type="nucleotide sequence ID" value="XM_018856158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7I0"/>
    <dbReference type="GeneID" id="30029134"/>
    <dbReference type="OrthoDB" id="10538600at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="175" mass="20034" checksum="89B84D6467BEC1F6" modified="2016-10-05" version="1">MGTETAKAHSAQLSKREKLRHFFLYRFKFPNEVFRRRSRRFFRKDFTDDGIDKPGAPIDPAQFQRSNESGGYRIIATTSNNDSPKRKCASKSAINGFRENYPAVQEDKLTAQSLADTSENRSLLKKIRLRWLQNPDFAPVVHPESGTRKLVPQSISFLKEMCPGPSTTEVSCSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7L0</accession>
    <name>A0A1A0H7L0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_209669</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710413.1">
        <property type="nucleotide sequence ID" value="XM_018855024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7L0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7L0"/>
    <dbReference type="GeneID" id="30028000"/>
    <dbReference type="OrthoDB" id="270584at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15898">
        <property type="entry name" value="EFh_PI-PLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="437"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="7"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="76"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="179"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="327"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="434"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="531" mass="59354" checksum="E25D765DA7398035" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8A9</accession>
    <name>A0A1A0H8A9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mitotic spindle checkpoint protein BUB3, WD repeat superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710737.1">
        <property type="nucleotide sequence ID" value="XM_018854199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8A9"/>
    <dbReference type="STRING" id="869754.A0A1A0H8A9"/>
    <dbReference type="GeneID" id="30027175"/>
    <dbReference type="OrthoDB" id="20669at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF12">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="376"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="535" mass="60413" checksum="16A75F52CD865AAB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8D1</accession>
    <name>A0A1A0H8D1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative GTP-binding protein engB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79434</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710673.1">
        <property type="nucleotide sequence ID" value="XM_018859066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8D1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8D1"/>
    <dbReference type="GeneID" id="30032042"/>
    <dbReference type="OrthoDB" id="391988at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052279">
        <property type="entry name" value="EngB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46498:SF1">
        <property type="entry name" value="GTP-BINDING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EngB-type G" evidence="5">
        <location>
            <begin position="128"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="349" mass="39630" checksum="D98F674DEB06E45C" modified="2016-10-05" version="1">MFLRRYASVSYLVGSRRSRPCSKNVPSERVSKYIYTPSDLTKYFRDDSYGHPLAAQITRADSLFYNARIKHEWTCASFEDIPDVKVARLSEERRQQVASVDPLRRTEYHENVAASRTSFGVDPQLLRPLPEVLLLGHTNAGKSTLINSLFVNKHQASTGNAATEYAYVSKRAGYTKCLNSYNVGNKLRVIDSPGYGRFGEPAQGQVVLDYIRRRQQLRRTFVIIDSLRGVCEEDAVLVEYLVDNGAPFEIIFTKVDVLMQKRFPKINMCPQKHDVDARAQAYERAKEGNSRVVQHFQHVIDKAGLRELATLPRLLFNNSHTSLLLARRHGFRDVRFAIAESCNLPTASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8D9</accession>
    <name>A0A1A0H8D9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">SWIRM-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_78779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMARCC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710901.1">
        <property type="nucleotide sequence ID" value="XM_018858938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8D9"/>
    <dbReference type="STRING" id="869754.A0A1A0H8D9"/>
    <dbReference type="GeneID" id="30031914"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048858">
        <property type="term" value="P:cell projection morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000020">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC2 isoform c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032451">
        <property type="entry name" value="SMARCC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381:SF1">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15381">
        <property type="entry name" value="CHONDROITIN SULFATE PROTEOGLYCAN 5 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16495">
        <property type="entry name" value="SWIRM-assoc_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="9">
        <location>
            <begin position="89"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="8">
        <location>
            <begin position="335"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="10">
        <location>
            <begin position="342"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="584" mass="65102" checksum="08C25A874D181A78" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8S8</accession>
    <name>A0A1A0H8S8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_195569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710809.1">
        <property type="nucleotide sequence ID" value="XM_018854867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8S8"/>
    <dbReference type="GeneID" id="30027843"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="179" mass="20263" checksum="902C47A570210488" modified="2016-10-05" version="1">MLGSWMLLGSWMLLSLGDIWLWGAWPWPYLDRLRADGIWGSWTYRGPWVSSEPVGFIRASGLHPSQWASSEPVGFIRACGLYASHGDLCFLGGRFLGVEILARSFFSALEIPVLSVMAMVCRVDGCSRSSWRYLVSVRWSQWTSLGSWALTIIPSVHGYWPIQPIHLAHRHGYWPIQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8T4</accession>
    <name>A0A1A0H8T4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78810</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710943.1">
        <property type="nucleotide sequence ID" value="XM_018858944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8T4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8T4"/>
    <dbReference type="GeneID" id="30031920"/>
    <dbReference type="OrthoDB" id="10249311at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990145">
        <property type="term" value="P:maintenance of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000019">
        <property type="entry name" value="40S ribosomal protein S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000530">
        <property type="entry name" value="Ribosomal_eS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047860">
        <property type="entry name" value="Ribosomal_eS12_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00972">
        <property type="entry name" value="RIBSOMALS12E"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01189">
        <property type="entry name" value="RIBOSOMAL_S12E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="27"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="15615" checksum="752C2310CC7D09EA" modified="2016-10-05" version="1">MSDIEEQQIVEEVAVEQQDASISIEDALKVVLRTSLVHDGLARGLREASKALSRKEAQLCVLCDSVTEASIVKLVEGLCNEGDEKIPLIKVSDAKLLGEWAGLCQLDREGNARKVVGASCVVVKNWGADSEERNILLEHFSQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9F3</accession>
    <name>A0A1A0H9F3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_200341</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711039.1">
        <property type="nucleotide sequence ID" value="XM_018854925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9F3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9F3"/>
    <dbReference type="GeneID" id="30027901"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09135">
        <property type="entry name" value="PLDc_PGS1_euk_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="192"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="562" mass="64481" checksum="21A914E4FE4F088C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9R6</accession>
    <name>A0A1A0H9R6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_201616</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711140.1">
        <property type="nucleotide sequence ID" value="XM_018854939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9R6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9R6"/>
    <dbReference type="GeneID" id="30027915"/>
    <dbReference type="OrthoDB" id="20729at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="350"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="482" mass="46556" checksum="A6FD23D4EA8B71BC" modified="2016-10-05" version="1">MQRQPCKFFQKGACRFGSLCKFLHADAGGDACDALQHGSVADFLRKTPDALGNSIKKDLAGLRRIQASPALTAYGLGPHAATNLIDGRDVSPEELRLQYMAAQAANSVDLYCRDLDLRRRDMDFCVAELTRNATLAARYQQMGVSNSGPVRPFIKKSLAESMRDLQAAGGSGAFGLAGFGLAGFGGSSGFGGSSGFGGPSNPGAFGGTAQPAPKSGLGAFGSASFGAGAAAPAQPAAGSGFGSAGFGGALGAGAFGSAGFGSGGASAGFGSSGFGSSGFGSAGFGSAGFGSAGSGSAGSGSSAAPAAPFGQASTLAFGAPSASAGTPASPFGAAAPAGGSFGASQNAFGASQPSSFGASQPSSFGASQPSSSGASNNAFGASQKAFGASNNAFGASNNAFGASPNSAFGAAPAPSAAFSQNMAPLGFGQPAPGFGAAPHHAAGLQPGGARLDNVDPMVREAFMASSFELGKIPDMAPPPEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9S0</accession>
    <name>A0A1A0H9S0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA pseudouridine synthase D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79039</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711283.1">
        <property type="nucleotide sequence ID" value="XM_018858991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9S0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9S0"/>
    <dbReference type="GeneID" id="30031967"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02576">
        <property type="entry name" value="PseudoU_synth_ScPUS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="5">
        <location>
            <begin position="370"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="103"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="694" mass="76252" checksum="F10C451E4969E3CB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9X6</accession>
    <name>A0A1A0H9X6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromatin modification-related protein EAF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_41411</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MRG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711444.1">
        <property type="nucleotide sequence ID" value="XM_018857396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9X6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9X6"/>
    <dbReference type="GeneID" id="30030372"/>
    <dbReference type="OrthoDB" id="124855at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990453">
        <property type="term" value="C:nucleosome disassembly/reassembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140003">
        <property type="term" value="F:histone H3K36me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060195">
        <property type="term" value="P:negative regulation of antisense RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043487">
        <property type="term" value="P:regulation of RNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.30">
        <property type="entry name" value="MRG domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008676">
        <property type="entry name" value="MRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038217">
        <property type="entry name" value="MRG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026541">
        <property type="entry name" value="MRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053820">
        <property type="entry name" value="MSL3_chromo-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880">
        <property type="entry name" value="MORTALITY FACTOR 4-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10880:SF15">
        <property type="entry name" value="MSL COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05712">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22732">
        <property type="entry name" value="MSL3_chromo-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038133">
        <property type="entry name" value="HAT_Nua4_EAF3/MRG15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51640">
        <property type="entry name" value="MRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MSL3 chromodomain-like" evidence="10">
        <location>
            <begin position="61"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="MRG" evidence="9">
        <location>
            <begin position="155"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF22732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="38182" checksum="0CD03872C4C8BD90" modified="2016-10-05" version="1">MVGGPQNFIPGSKVLAYHGPLVYEAKVLKYHKEGLELVDVEEEKSEPLDKNKIPGFLLDKNAYFLHYKGWNPKWDEWVSSERIIEFNDDNLGLSRELRNARKKTVERLDSGKEAKAEAAEQKSRRGRRKNAPTEASTNHSGPSKDQSKPNSNGRKRQKTEPHAGYEVMLPFSPRLKCVMVDDWELITKDRKLVNLETVTPVLTILKEYYEWKRAGQSIELTRTTEEALSGLKILFDQTFGLDLLYKFERLQYSELFRTRKNFEPSNVYGCEHLLRLIITLPGRVSQTVMDAMSVNIMMGQMSDLLEYIDANMATLGSSYMNVSSQYDRVARM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Z0</accession>
    <name>A0A1A0H9Z0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Synaptobrevin homolog YKT6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_43877</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711210.1">
        <property type="nucleotide sequence ID" value="XM_018857709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Z0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Z0"/>
    <dbReference type="GeneID" id="30030685"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033106">
        <property type="term" value="C:cis-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016409">
        <property type="term" value="F:palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061909">
        <property type="term" value="P:autophagosome-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15867">
        <property type="entry name" value="R-SNARE_YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000020">
        <property type="entry name" value="synaptobrevin homolog YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045848">
        <property type="entry name" value="R-SNARE_YKT6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Longin" evidence="11">
        <location>
            <begin position="7"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="139"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="199" mass="22534" checksum="E163E7B4A9990BEE" modified="2016-10-05" version="1">MKIYYIGILKIGDKAIELSSASDLSQFSFFERSSVAQFMTFFSETVSQRTPAGQRQSIEEGNYVGHTYTRSEGLSCVVITDKEYPVRPAYTLINKILEEYLSLHPASQWNAVTEPSASLQYDQLAVYLKKYQDPSQADSIMKVQQELDETKIVLHKTIESVLQRGEKLDSLVDKSEALSLSSRMFYKQAKKTNSCCVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Z3</accession>
    <name>A0A1A0H9Z3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_203192</name>
        <name evidence="2" type="ORF">METBIDRAFT_203238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711215.1">
        <property type="nucleotide sequence ID" value="XM_018854957.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711219.1">
        <property type="nucleotide sequence ID" value="XM_018854959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Z3"/>
    <dbReference type="GeneID" id="30027933"/>
    <dbReference type="GeneID" id="30027935"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="108" mass="12360" checksum="D14685AC65EEA0ED" modified="2016-10-05" version="1">MATLGPVSNAQPAAFGLAKITIQNIFASSTYRHKKSGVVKAEFVSHESLFGRTKQQLSFRLIGRRPVEHHFVRSYRKFSWAVRLLFHQHARTQKFKFMPSGPETETTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA51</accession>
    <name>A0A1A0HA51_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_20078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711390.1">
        <property type="nucleotide sequence ID" value="XM_018854933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA51"/>
    <dbReference type="GeneID" id="30027909"/>
    <dbReference type="OrthoDB" id="4026192at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="19160" checksum="8F4FA3312E5CD114" modified="2016-10-05" version="1" fragment="single">ATTPLPLPVVTLSVDLSSTKKNMASVEQSFFDNWGAGGADTASQALVATPHDADPDVPDKLAILSTPEDLMEYLVYLSLHKKSVELAYEVSKNRLVSSGWCSGHDIENLNLQRDSSLSQIDSKLLQIEARLNSEFNMSFLNSKHMQQVSPTQKCAPRAVMSPSLKDLENKCLYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA63</accession>
    <name>A0A1A0HA63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome-releasing factor 2, mitochondrial</fullName>
            <shortName evidence="5">RRF2mt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Elongation factor G 2, mitochondrial</fullName>
            <shortName evidence="5">EF-G2mt</shortName>
            <shortName evidence="5">mEF-G 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MEF2</name>
        <name evidence="7" type="ORF">METBIDRAFT_43891</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711289.1">
        <property type="nucleotide sequence ID" value="XM_018857712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA63"/>
    <dbReference type="STRING" id="869754.A0A1A0HA63"/>
    <dbReference type="GeneID" id="30030688"/>
    <dbReference type="OrthoDB" id="198619at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051881">
        <property type="term" value="P:regulation of mitochondrial membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000514">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03059">
        <property type="entry name" value="mEF_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030851">
        <property type="entry name" value="EFG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="38"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="111"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="165"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="845" mass="92340" checksum="A352D5A4B3023493" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAI7</accession>
    <name>A0A1A0HAI7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_31743</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711654.1">
        <property type="nucleotide sequence ID" value="XM_018855905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAI7"/>
    <dbReference type="STRING" id="869754.A0A1A0HAI7"/>
    <dbReference type="GeneID" id="30028881"/>
    <dbReference type="OrthoDB" id="2501249at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070124">
        <property type="term" value="P:mitochondrial translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052837">
        <property type="entry name" value="Mitoribosomal_bS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_bS21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN MRP21, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41237:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="175" mass="19875" checksum="EB24C09EF418676C" modified="2016-10-05" version="1">MFTNVTGRLPALRQCTPRNAFVRFISQKKEADPFSELKDTAASAPKNASTIFNFDALIANSPDLRDTQRSLGKYDEYAFGNSVKDPREAAKAINISGPSAGRTVDVTFQNVGTALRGVQSILRQNDIRSQWHNQKRYIRPAKFRKMKKSLWWKKHFALGFSDLMSQITDAKRRGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAK5</accession>
    <name>A0A1A0HAK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDENN FLCN/SMCR8-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_78035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711417.1">
        <property type="nucleotide sequence ID" value="XM_018858805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAK5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAK5"/>
    <dbReference type="GeneID" id="30031781"/>
    <dbReference type="OrthoDB" id="5599713at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990877">
        <property type="term" value="C:FNIP-folliculin RagC/D GAP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037521">
        <property type="entry name" value="FLCN/SMCR8_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021713">
        <property type="entry name" value="Folliculin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037520">
        <property type="entry name" value="Folliculin/SMCR8_longin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441:SF2">
        <property type="entry name" value="FOLLICULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31441">
        <property type="entry name" value="FOLLICULIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11704">
        <property type="entry name" value="Folliculin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51834">
        <property type="entry name" value="DENN_FLCN_SMCR8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN FLCN/SMCR8-type" evidence="1">
        <location>
            <begin position="43"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="220" mass="24876" checksum="866A13E849189B0D" modified="2016-10-05" version="1">MPSPRFVVCLAHFCEIHGPSTIICTQRQTSTGPMLTSTLSLCESCALILPKDATNIVTDVEYGASPQCYISTKYPQSPRLYTSLMKLVMKCLSVEATADPLKPLFYGDATNGFCLSKVFSIPDLHARGAERKYAFLVLCDSETRLLERWHFVSSYISEMIAFLQTKVEAVRDQAKIESLDNERYLRRLKNMPRSLVELAGDKELFVRLHLCGIELLRDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAM6</accession>
    <name>A0A1A0HAM6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_78044</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711442.1">
        <property type="nucleotide sequence ID" value="XM_018858807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAM6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAM6"/>
    <dbReference type="GeneID" id="30031783"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000160">
        <property type="entry name" value="Casein kinase I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="14"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="315"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="427" mass="48664" checksum="3CFBDB9745ED46B4" modified="2016-10-05" version="1">MAQSSGEHRVGVHYKVGKKIGEGSFGIIFEGINLLNNQQIAIKFEPRKCEAPQLRDEYRSYRILSEAAGVPKAYFFGQEGVHNILVIDLLGPSLEDLFDWCGRKFSTKTVAQVAKQMLQRMQSIHEHNLIYRDVKPDNFLIGKPSTPQANMVYVVDFGMAKQYRDPKTKTHIPYKEKKALSGTARYMSINTHLGREQLRRDDLESLGHVFMYFLRGHLPWQGLKAPTNKQKYEKIGIKKQTTSINELCYGFPIQFAQYLTYVRNLRFDEEPDYDYLLGLMDKALISIDEKADGHYDWMDLNGGKGWDAALNKKANLHGYGNPHPPQRQRKATNNTNSNAAVQSKRAPLVSASPNQPLMPLQQRLSRSQSMTHANYNSTMDSNGAKAGPLHHNRVLAPHEDAELHSDTSSFDARRNKKSWIRSVFCCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAW4</accession>
    <name>A0A1A0HAW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="11">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
            <ecNumber evidence="11">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">COQ6</name>
        <name evidence="13" type="ORF">METBIDRAFT_41774</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-polyprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoic acid. Also acts downstream of coq4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-polyprenyl)phenol to 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to coq6 from NADPH via a ferredoxin/ferredoxin reductase system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81183"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 4-hydroxy-3-(all-trans-polyprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = a 3,4-dihydroxy-5-(all-trans-polyprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81195"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10930"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:64694"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="11"/>
    <dbReference type="EC" id="1.14.15.46" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711532.1">
        <property type="nucleotide sequence ID" value="XM_018857450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAW4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAW4"/>
    <dbReference type="GeneID" id="30030426"/>
    <dbReference type="OrthoDB" id="683240at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120538">
        <property type="term" value="F:2-methoxy-6-polyprenolphenol 4-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106364">
        <property type="term" value="F:4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000021">
        <property type="entry name" value="Ubiquinone biosynthesis monooxygenase COQ6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03193">
        <property type="entry name" value="COQ6_monooxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051205">
        <property type="entry name" value="UbiH/COQ6_monooxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000689">
        <property type="entry name" value="UbQ_mOase_COQ6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43876:SF7">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0274">FAD</keyword>
    <keyword evidence="3 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4 11" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="FAD-binding" evidence="12">
        <location>
            <begin position="174"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="483" mass="52488" checksum="12FD7B33A4CA8AC3" modified="2016-10-05" version="1">MRSFLRLLSAAARPVLQDVVIVGGGPAGLSLMAALKNSPKTQHLLCTLVEGLDLLAARDFVALPPEAFSNRIVSLTPKSVQFMKEKSGSWPFLQEARVQFYDNMIAYDSQDAAARIHFDSSTLRGAGDADGGDLDMSSAIAAMCEVVHIQGSLLGKLDELKEEFGDEDDGLRIVDRAKVVDITQSAENAGLDWPVVTLDSGEQLQARLLVGADGQNSPVRKYADIASRGWSYERFGVVGTVKLQFEDYRAVAWQRFLTTGPLAILPLPGDNATFVWSTTPELAAILQQTDPEIFPHLLNAAMTLDEVDLNYLYGVLKADPADTRVIQDIAWRTLKMLEADLDENFPVPIRALVPGSRARFPLKMLQADAYMAPRVALVGDAAHTVHPLAGQGLNMGQLDVAALVQALETGVDRGLDIGSTLVLEKYNADAWPANHMLMGVCDKLHKVFSTDFGPLVFLRGTGLKTLNAFDSVKQLMIKSISGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB19</accession>
    <name>A0A1A0HB19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711695.1">
        <property type="nucleotide sequence ID" value="XM_018855911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB19"/>
    <dbReference type="STRING" id="869754.A0A1A0HB19"/>
    <dbReference type="GeneID" id="30028887"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="113"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="35643" checksum="E1DF6D18BAD635ED" modified="2016-10-05" version="1">MSSTLHLVDLVDTFPYETNEFYWTLTTCCEQPLGYITPDIAKRFTRNDHAHYFDVDMAIRRVKISNKLDTIEKRDAAFKEIALFWRRTDPSLEKGWRDELYTVYFPKATPYAHLERAFACLLGVVTYGVHITGYVPADKTRDNTLKIWVPKRSATKQTYPGKLDNTIAGGLAYPYSITENAIKECYEEGGLSESFVKQNLVGAGVLSYMCQPFGPKGHVQPEVEYIYDLIFDSETDNIPQPIDGEAEDFKLMLITEVHQRILNDEFKPNSALVIVDFMIRHGYLSWENEPHYLEIVSRIHRKLPFSTMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB58</accession>
    <name>A0A1A0HB58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">A Q resistance multidrug resistance transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78276</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. CAR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711756.1">
        <property type="nucleotide sequence ID" value="XM_018858853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB58"/>
    <dbReference type="STRING" id="869754.A0A1A0HB58"/>
    <dbReference type="GeneID" id="30031829"/>
    <dbReference type="OrthoDB" id="3936150at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015203">
        <property type="term" value="F:polyamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010509">
        <property type="term" value="P:intracellular polyamine homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000009">
        <property type="entry name" value="MFS multidrug transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF5">
        <property type="entry name" value="QUINIDINE RESISTANCE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="332"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="49"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="554" mass="61608" checksum="ED600AD58CBE37F2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB72</accession>
    <name>A0A1A0HB72_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_11693</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711637.1">
        <property type="nucleotide sequence ID" value="XM_018854063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB72"/>
    <dbReference type="STRING" id="869754.A0A1A0HB72"/>
    <dbReference type="GeneID" id="30027039"/>
    <dbReference type="OrthoDB" id="6021263at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024388">
        <property type="entry name" value="Ribosomal_mL58"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L20, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28266:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12824">
        <property type="entry name" value="MRP-L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="180" mass="20758" checksum="4E0BEFB11FFCD7B3" modified="2016-10-05" version="1">MLRTATRNISIKSLPHELQPKNKYNAVRSKFNMKPVPTQGLIYNPPASMPTVKETPRAFLPPNDPRLKLLAEKFKTYTPEELEEMPVIYATKKDNSLTPEIVQEIIALRSEDPDKWSIAKLAAKFSVDAKKVNVITGSSKERQQKVLAQLAEQQSQWTGKKRLARDDRVKRKQMWLRNEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBV7</accession>
    <name>A0A1A0HBV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase Rpb4/RPC9 core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78353</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711883.1">
        <property type="nucleotide sequence ID" value="XM_018858869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBV7"/>
    <dbReference type="STRING" id="869754.A0A1A0HBV7"/>
    <dbReference type="GeneID" id="30031845"/>
    <dbReference type="OrthoDB" id="2186918at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990328">
        <property type="term" value="C:RPB4-RPB7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045222">
        <property type="entry name" value="Rpb4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21297">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="6">
        <location>
            <begin position="59"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="61"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="192" mass="21692" checksum="B6DDF0CEE96E1B73" modified="2016-10-05" version="1">MNVSTSALGVRRRKAKGQNIDDEENASLLRLGPEFQRTQITNSGDEEQLIALNLSEARLLVRAALKERKLKSEKNQMLEDEDEEENEREDEIANIELAGPNSNEIVHKTLNYLSTFSRFKNPSSTETVEKLLNDFGTHASEPLHPFELAQLGNLECEDSEEAKSLIPSLTNKVSDVQLKTLLTELRKYHTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBX3</accession>
    <name>A0A1A0HBX3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2',3'-cyclic-nucleotide 3'-phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_12012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the metabolism of ADP-ribose 1',2'-cyclic phosphate which is produced as a consequence of tRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. CPD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.37" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712021.1">
        <property type="nucleotide sequence ID" value="XM_018854147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBX3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBX3"/>
    <dbReference type="GeneID" id="30027123"/>
    <dbReference type="OrthoDB" id="514292at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009187">
        <property type="term" value="P:cyclic nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012386">
        <property type="entry name" value="Cyclic-nucl_3Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28141:SF1">
        <property type="entry name" value="2',3'-CYCLIC-NUCLEOTIDE 3'-PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07823">
        <property type="entry name" value="CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="261" mass="29559" checksum="E62B31BDC990F08D" modified="2016-10-05" version="1">MGVALWLCPKKNTPLYDKLQVLMNSLSTVFPGLPPRFEPHITITSNVAVDLANAKDDVDRILLACCIALDSLPRDSSDPHWIHLGRVASQKRFFKKLYFQVLRDPTLVSFARIVRELFVVLPERTELEHKVKNPHLFHTDHLGNIVRRKPGLRARGHPHVDAALDHAKIQSELAHEAASWSAAEFDPHLSLVYSDLHPIDNALWHTIRSRVSDYLSLEDCDLEEWDVRGNGLSWEGGVLKLVLCEGDVNEWPVLGSVDLHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC14</accession>
    <name>A0A1A0HC14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA repair and recombination protein RAD52</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_25985</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711927.1">
        <property type="nucleotide sequence ID" value="XM_018855307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC14"/>
    <dbReference type="STRING" id="869754.A0A1A0HC14"/>
    <dbReference type="GeneID" id="30028283"/>
    <dbReference type="OrthoDB" id="206565at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="157" mass="17426" checksum="B6147C16DE34D72B" modified="2016-10-05" version="1" fragment="single">VLFPSLSLFEETSDCESGPEPHFLSPWAKTKIGTFQARLELVLRKRELRGVGKYGISSVSSALLINMVNESFGFNGWLSQILDCHIASQNFDDEKNEYSMVQTATVRIVLDDGTEVEAKGNGEVRGLPSKYQCLGTSRKMACTDGLRNAFLKFPDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC43</accession>
    <name>A0A1A0HC43_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Required for respiratory growth protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for respiratory activity and maintenance and expression of the mitochondrial genome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRG9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711957.1">
        <property type="nucleotide sequence ID" value="XM_018857347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC43"/>
    <dbReference type="STRING" id="869754.A0A1A0HC43"/>
    <dbReference type="GeneID" id="30030323"/>
    <dbReference type="OrthoDB" id="5578174at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF3">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="111"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="147"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="191" mass="22077" checksum="40F98D1DAD538471" modified="2016-10-05" version="1">MWNFLRQPCAPKARLWRAHGTLALTPSELRFKQKLEAAERERRQHAPEWEKREQTLRKRYGTWNPTHKLSRQQMNDIRDLKARAPGLKTVQLADFFKISPESIRRILKSSWVPSEAERAKQEARGEKRKTESSGKSYESVKSYGSGKSYGSGAPGHRGGPKYKHQKHGPASRDADRRWRKPFTEGVGDLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC76</accession>
    <name>A0A1A0HC76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L39</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712121.1">
        <property type="nucleotide sequence ID" value="XM_018857431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC76"/>
    <dbReference type="STRING" id="869754.A0A1A0HC76"/>
    <dbReference type="GeneID" id="30030407"/>
    <dbReference type="OrthoDB" id="6332053at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_eL39_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19970">
        <property type="entry name" value="RIBOSOMAL PROTEIN L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="59" mass="7146" checksum="9A3A7E731A70172A" modified="2016-10-05" version="1">MTEPHTNYLQANKSFKVKQKLAKASKQNRPLPQWIRLKTDNTIRYNAKRRHWRRTKLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCD6</accession>
    <name>A0A1A0HCD6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_12042</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712057.1">
        <property type="nucleotide sequence ID" value="XM_018854153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCD6"/>
    <dbReference type="GeneID" id="30027129"/>
    <dbReference type="OrthoDB" id="434783at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051752">
        <property type="entry name" value="Mito_2-oxodicarb_carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46356:SF1">
        <property type="entry name" value="MITOCHONDRIAL 2-OXODICARBOXYLATE CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="102"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="195"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="31581" checksum="94B2BADE15F27518" modified="2016-10-05" version="1">MSDPKPLPFMYQFLSGAIAGVSEIMVMYPLDVVKTRQQLATTADYNGTIRCLKKIVAEEGFSRLYKGISAPILMEAPKRAIKFAANDEWGKFYRNAFDVPKMTQPLAVLTGATAGATETFVVVPFELVKIQLQDRTSKFNSAGEVVKHIIKSDGILGLYKGLESTMWRHVAWNAGYFGLIHQVRTWMPQPKSSSEKTLIDLTCGTIGCTFGTMLNTPFDVVKSRIQAGSSKYIWTIPSVLTVAKEEGVGALYKGFIPKVLRLGPGGGILLVVFTTCMDFFRTLQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCJ8</accession>
    <name>A0A1A0HCJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Carboxypeptidase</fullName>
            <ecNumber evidence="13">3.4.16.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_32129</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Involved in degradation of small peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Release of a C-terminal amino acid with broad specificity.</text>
            <dbReference type="EC" id="3.4.16.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase S10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.-" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712158.1">
        <property type="nucleotide sequence ID" value="XM_018855986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCJ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HCJ8"/>
    <dbReference type="GeneID" id="30028962"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046938">
        <property type="term" value="P:phytochelatin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000001">
        <property type="entry name" value="Carboxypeptidase Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008442">
        <property type="entry name" value="Propeptide_carboxypepY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033124">
        <property type="entry name" value="Ser_caboxypep_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018202">
        <property type="entry name" value="Ser_caboxypep_ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF113">
        <property type="entry name" value="SERINE CARBOXYPEPTIDASE CTSA-4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05388">
        <property type="entry name" value="Carbpep_Y_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00560">
        <property type="entry name" value="CARBOXYPEPT_SER_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00131">
        <property type="entry name" value="CARBOXYPEPT_SER_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444432" description="Carboxypeptidase" evidence="13">
        <location>
            <begin position="19"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="Propeptide carboxypeptidase Y" evidence="14">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="545" mass="60917" checksum="888DE9CB2EE141EA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCK6</accession>
    <name>A0A1A0HCK6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RabGAP/TBC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_77400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712335.1">
        <property type="nucleotide sequence ID" value="XM_018858691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCK6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCK6"/>
    <dbReference type="GeneID" id="30031667"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="175"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="549" mass="62334" checksum="FFE9712BC4AD52A6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCM3</accession>
    <name>A0A1A0HCM3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_32200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712246.1">
        <property type="nucleotide sequence ID" value="XM_018856003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCM3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCM3"/>
    <dbReference type="GeneID" id="30028979"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15710:SF243">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PRAJA-2 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="112"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="187"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="201" mass="22891" checksum="5AECED0121CAB5A5" modified="2016-10-05" version="1">MSTYESDHNLNTSESINSQNDGRRQYHSLSSAIDAFLTTTAASNRERLSGREIFENALRMMGDEENSEVIQQFLQNLDSEGTEINTSVGVDDAFLDALERVDLDSLSETADCPICTNKFVHNKYPLIVKLPCFTNGKSSKQHIFDMDCIAPWLKVNSTCPMCRCDMHDAKKARREHLEAELRRAREQEEEDEEDDDRGLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCV5</accession>
    <name>A0A1A0HCV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30918</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712425.1">
        <property type="nucleotide sequence ID" value="XM_018855737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCV5"/>
    <dbReference type="GeneID" id="30028713"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="71" mass="8203" checksum="C04955933B166A4C" modified="2016-10-05" version="1" fragment="single">MPKHTYPTTKHIYPHPSISIHTQAYLSTPKHTIPILNLIYPRPIHTHTLRQANTVPNHIHPNRPTQDAHPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCX7</accession>
    <name>A0A1A0HCX7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712445.1">
        <property type="nucleotide sequence ID" value="XM_018853863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCX7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCX7"/>
    <dbReference type="GeneID" id="30026839"/>
    <dbReference type="OrthoDB" id="273181at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008138">
        <property type="term" value="F:protein tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053239">
        <property type="entry name" value="Dual_spec_PTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47550:SF1">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE PPS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="1">
        <location>
            <begin position="676"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="793" mass="91127" checksum="E4387D00A3B2F611" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD77</accession>
    <name>A0A1A0HD77_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_19111</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712348.1">
        <property type="nucleotide sequence ID" value="XM_018854845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD77"/>
    <dbReference type="STRING" id="869754.A0A1A0HD77"/>
    <dbReference type="GeneID" id="30027821"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="199" mass="21738" checksum="70F73D8B0D3EEB58" modified="2016-10-05" version="1" fragment="single">IDRNTIRNTSSEIRLANLTVNQEVYYSIVNCSLINVSGCVSNAGNIHVISLDGEQIWVRMTGEEFENSGIVAINALRSNAISNFCFNLSGSFLNTGNMYFGINGSISGSLPFSVTSVNSWSNTGKMIFHAAHGEKARLRIRRYVADDVTNSISNNGTICLNNTLWPVHTNIEGNGCIAVGMYGQIDLLFSKSTYHISET</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD83</accession>
    <name>A0A1A0HD83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DCG1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_138567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuE racemase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712353.1">
        <property type="nucleotide sequence ID" value="XM_018854436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD83"/>
    <dbReference type="STRING" id="869754.A0A1A0HD83"/>
    <dbReference type="GeneID" id="30027412"/>
    <dbReference type="OrthoDB" id="412018at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052186">
        <property type="entry name" value="Hydantoin_racemase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053714">
        <property type="entry name" value="Iso_Racemase_Enz_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28047:SF5">
        <property type="entry name" value="PROTEIN DCG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="24853" checksum="F9076518A3F339F5" modified="2016-10-05" version="1">MPSILVINPNSSTKVTDGLRAILNAPAGITLEFYTAPASSPREIDGAQSLVLSEQATLPDLVKKNLHTAHDGYLVCCYSDHPLVNSLAQITRLPVLGIMQATLLYSYANARLQKLFVLTSTSGWNPLLDEGITAFAGTGVFPLHKFQKTRALDVSVLSLADPAEFKKISDRLDFFLQEYKDDHIDCVLLGCAGMAGLDDKLSAMYPHIEFVDSCKAGVAFLVSLMSFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDG4</accession>
    <name>A0A1A0HDG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_10876</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712464.1">
        <property type="nucleotide sequence ID" value="XM_018853869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDG4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDG4"/>
    <dbReference type="GeneID" id="30026845"/>
    <dbReference type="OrthoDB" id="47059at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048682">
        <property type="entry name" value="COG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048684">
        <property type="entry name" value="COG4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013167">
        <property type="entry name" value="COG4_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048680">
        <property type="entry name" value="COG4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24016:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20662">
        <property type="entry name" value="COG4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08318">
        <property type="entry name" value="COG4_m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20663">
        <property type="entry name" value="COG4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00762">
        <property type="entry name" value="Cog4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="COG4 transport protein middle alpha-helical bundle" evidence="9">
        <location>
            <begin position="196"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="852" mass="94741" checksum="9D1EB749F16C6AEE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDL8</accession>
    <name>A0A1A0HDL8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Protein ROT1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_146502</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for normal levels of the cell wall 1,6-beta-glucan. Involved in a protein folding machinery chaperoning proteins acting in various physiological processes including cell wall synthesis and lysis of autophagic bodies.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ROT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712607.1">
        <property type="nucleotide sequence ID" value="XM_018854526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDL8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDL8"/>
    <dbReference type="GeneID" id="30027502"/>
    <dbReference type="OrthoDB" id="5327821at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019623">
        <property type="entry name" value="Rot1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28090:SF1">
        <property type="entry name" value="PROTEIN ROT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10681">
        <property type="entry name" value="Rot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017290">
        <property type="entry name" value="ROT1_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291756" description="Protein ROT1" evidence="11">
        <location>
            <begin position="20"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR017290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="27136" checksum="3E8B37DD95768500" modified="2016-10-05" version="1">MKSFSAFLGALCVLQLVAAEPNMEELEGTWTSKSNTVFTGPDYYDPVDELLIEPALPGISYSFTSDGYYEEALYRITSNGVDHSCPIASMTYQHGTYELLSNGSLVLTPIAVDGRQLLSDPCGSSSSNAVYSRYVQSTWFEKYQVSVSSYYGRYMLQIYQFDGSKMQPLYLAYKPPVMLPTYALNPTDAASETSSSLRRRIKRSLENLYRTSAVRDFSSPKLDMFWWAAVGVLGASSAAYVLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW0</accession>
    <name>A0A1A0HDW0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712578.1">
        <property type="nucleotide sequence ID" value="XM_018853892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW0"/>
    <dbReference type="GeneID" id="30026868"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="203" mass="22786" checksum="05DB1F7FB0E80C28" modified="2016-10-05" version="1">MEDLAEDNRNKATHCSTEKLPKTEILTPPLEENNSKDSAKKLSAITVDTTCDTNIYEKQARVSDPEQASGLPLDSSQEDNTATNHDDLYLSRLRKHLKHKRQTRTTLASVSVKNDCGMPTFAPAYTCQFDTETTEENEGWQVQRKRRLRGSRLLVNHGLHSSRATSSTLGPVEKVSLLDSYGGMEPIPENIVILYKRKRTDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDY1</accession>
    <name>A0A1A0HDY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="4">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Isopentenyl-diphosphate delta-isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_31159</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:23285"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712717.1">
        <property type="nucleotide sequence ID" value="XM_018855782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDY1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDY1"/>
    <dbReference type="GeneID" id="30028758"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000012">
        <property type="entry name" value="Isopentenyl-diphosphate Delta-isomerase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="16">
        <location>
            <begin position="89"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="280" mass="32331" checksum="4401F90F86FD119F" modified="2016-10-05" version="1">MSSYQELVKCLTRLNILDKWTDVTPVENISGVPIDAGSTSAHENVFEGHDEEQIRLMEELCIVLDYEDHPIGAGTKKLCHLMSNINRGLLHRAFSVFLFNEDGKLMLQQRADEKITFPNMWTNTCCSHPLCVPSELGIKDNKSNKNELETAVAGVKLAAQRKLYHELGISEQDCPVSSFQFLTRIHYKSASGDEASEWGEHEIDYILILRAKNNIKVEPSLNEVKDFMYVSVDELKVMFEDKNLQFTPWFKLICETFLFQWWADLKNIEQFEDKKIHRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB3</accession>
    <name>A0A1A0HEB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABC1 atypical kinase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712739.1">
        <property type="nucleotide sequence ID" value="XM_018855792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEB3"/>
    <dbReference type="GeneID" id="30028768"/>
    <dbReference type="OrthoDB" id="1290869at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044289">
        <property type="term" value="C:mitochondrial inner-outer membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055091">
        <property type="term" value="P:phospholipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052402">
        <property type="entry name" value="ADCK_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890:SF1">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45890">
        <property type="entry name" value="AARF DOMAIN CONTAINING KINASE 2 (PREDICTED)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="381" mass="44139" checksum="E8BD11239204ADC9" modified="2016-10-05" version="1">MKFFANLVDVIPTMEWLSLPDEVEQFSILMRLQLDLRIEALNLAKFRHNFRNRLDIHFPKPFLNFSTREVLVEEYIKAIPMEKLLSLSGNFGKNLSKEVSDKGLDSFLQMLILDNFVHADLHPGNMLIRFYRNEMYTHEREYKIVKSTNENETNRITEELLSLGNDSQAWCNKLESLYENGYHAEICFLDVGLITELNHDDRINFIDLFKALSEFDGYKAGDLMVERSRTPETVVNKEIFALKVEKLVDRMKERTFTLGNISIGDLLDQVLSMVRSHHVRMEGDFITVIVAILLLEGMGRQLDPQLDLFASSLPVLRKLGILKEGKELLQNEDSLNMMKVWLALEVRQLINASIQDVSFFLCFILSPVSKIFGKVTQQFSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HED1</accession>
    <name>A0A1A0HED1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712847.1">
        <property type="nucleotide sequence ID" value="XM_018855822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HED1"/>
    <dbReference type="STRING" id="869754.A0A1A0HED1"/>
    <dbReference type="GeneID" id="30028798"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="91" mass="10980" checksum="06FD216C938B1779" modified="2016-10-05" version="1">MLGFYQDLLDMESVRVENPHLETIWHEDVHKLAVHQNVEYGQQKAKFMRWFLFDLLSCEGKYTHTARFGLNMAYTRQMAHGYLITRPWFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEE0</accession>
    <name>A0A1A0HEE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribophorin II C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_39983</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712975.1">
        <property type="nucleotide sequence ID" value="XM_018857213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEE0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEE0"/>
    <dbReference type="GeneID" id="30030189"/>
    <dbReference type="OrthoDB" id="432292at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044236750" description="Ribophorin II C-terminal domain-containing protein" evidence="8">
        <location>
            <begin position="21"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="9">
        <location>
            <begin position="165"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="270" mass="28725" checksum="C284B3FAE53174E2" modified="2016-10-05" version="1">MKFSLTLALLALSLVSTAAAYAVTGGSVYVNRGSLLEIDPNSKDTHRITLNSAKDVVSFSLAVQGDHKPHQLMYMLSDNNGLDHAVFAVYKDGQAVADISANKVPSALKKSDRIFVSLIAADALEEEANVIFPVVELIPSDALKESVGSDGPVRLGAQPEIHHVFNTEEPTVNGVVPVVFSAFAVALFVFLVVTWTNVLGNELFAGSPGATWNAALLATLASFEYTFFKYYMGASIFTTAFNSMVLTAPALFFGSRALKSLGQARGDGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEF1</accession>
    <name>A0A1A0HEF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_77789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712867.1">
        <property type="nucleotide sequence ID" value="XM_018858765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEF1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEF1"/>
    <dbReference type="GeneID" id="30031741"/>
    <dbReference type="OrthoDB" id="4055624at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018843">
        <property type="entry name" value="Utp8_b-prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053881">
        <property type="entry name" value="Utp8_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10395">
        <property type="entry name" value="Utp8_b_propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22542">
        <property type="entry name" value="Utp8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Utp8 beta-propeller" evidence="1">
        <location>
            <begin position="6"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Utp8 C-terminal" evidence="2">
        <location>
            <begin position="406"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="736" mass="79232" checksum="AE9677A3FA9077DA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEG7</accession>
    <name>A0A1A0HEG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_152570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712876.1">
        <property type="nucleotide sequence ID" value="XM_018854578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEG7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEG7"/>
    <dbReference type="GeneID" id="30027554"/>
    <dbReference type="OrthoDB" id="408631at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050466">
        <property type="entry name" value="Carboxylest/Gibb_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024:SF24">
        <property type="entry name" value="ALPHA_BETA HYDROLASE FOLD-3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23024">
        <property type="entry name" value="ARYLACETAMIDE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha/beta hydrolase fold-3" evidence="1">
        <location>
            <begin position="99"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="346" mass="38717" checksum="C7306AAF8EE23E84" modified="2016-10-05" version="1">MASTHEALNPIHPAVLAKMDPVFVAMYNEHVAKIPNAPIDLHKLRANYSKIYSYGTGAYPHIENVSEISFLSFDGAPVKLRIYRPNDAAADDFLPVHIDFHGGGGGLGDLETEKHVLCHYVQLARVAVVDVDYRLVPDYPYPTYIQDCFWATKFVYENAASLNFSKDLISVGGVSVGGNVALIVSHLARDAQIPLVLCLAGTPQVDDVESYKSAQDSPYESVREFEFAPTLNWSRITYFDKLKWQTLPTDATERARQLEKIGWFKNVMLAPDFAVLPKTVVVTAGCDPMRDEGEAYAAKLIKTGNEVVFKRYPGVPHPFMHMDSYLWQASDYIQFTAQELKRAHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEZ1</accession>
    <name>A0A1A0HEZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Apses-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_14342</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFG1/PHD1/stuA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713170.1">
        <property type="nucleotide sequence ID" value="XM_018854497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEZ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEZ1"/>
    <dbReference type="GeneID" id="30027473"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000003">
        <property type="entry name" value="Ascospore maturation 1 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029790">
        <property type="entry name" value="EFG1/Phd1/StuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47792:SF1">
        <property type="entry name" value="PROTEIN SOK2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="112" mass="12975" checksum="9A8234D18EE0FBCF" modified="2016-10-05" version="1" fragment="single">YQPAGIRPRVTTTMWEDEKTLCYQVDANNVSVVRRADNNMINGTKLLNVAQMTRGRRDGILKLEKIRHVVKIGSMHLKGVWIPFDRALAMAQRERIVDLLYPLFVRDIKKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF60</accession>
    <name>A0A1A0HF60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ricin B lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31491</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713116.1">
        <property type="nucleotide sequence ID" value="XM_018855862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF60"/>
    <dbReference type="GeneID" id="30028838"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012678373" description="Ricin B lectin domain-containing protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16651" checksum="81A8CE824A8768E7" modified="2016-10-05" version="1">MKLLATLPLFSLVLAAPRLIPDIKPLAGILSVKNAKTPEQLGSGFISYNGSVSKKIEPHMFFAEKGHVYIQLLGEDGQNANRLFMGLDAEKKLVMKDYAETNVKIIEDIHCKDFFLSANNGGIFQVCEDGLIGHNSGCESTETIEIQWIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ6</accession>
    <name>A0A1A0HFQ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29535</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713470.1">
        <property type="nucleotide sequence ID" value="XM_018855429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFQ6"/>
    <dbReference type="GeneID" id="30028405"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008079">
        <property type="term" value="F:translation termination factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18626">
        <property type="entry name" value="CD_eEF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.990:FF:000002">
        <property type="entry name" value="mRNA export factor elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000193">
        <property type="entry name" value="Probable Elongation factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015688">
        <property type="entry name" value="eEF3_ABC2_chromodomain-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047038">
        <property type="entry name" value="eEF3_chromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056891">
        <property type="entry name" value="NEW1_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24988">
        <property type="entry name" value="NEW1_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="561"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="803"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="933"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1113"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1150"/>
            <end position="1160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1186" mass="130401" checksum="5119DBC181E348F6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ7</accession>
    <name>A0A1A0HFQ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713301.1">
        <property type="nucleotide sequence ID" value="XM_018855395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFQ7"/>
    <dbReference type="GeneID" id="30028371"/>
    <dbReference type="OrthoDB" id="6161264at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="115" mass="12899" checksum="C25A92735C72227E" modified="2016-10-05" version="1">MVGGRSSVRTRLRGAGRTLVNRDSWGKVTIVFRNGGYLERMSIVENKSTVKNKSRVENKSRVENKSTVKNKSRVENKSTVENKSTVKNKSRVENKSRVENRSSVQSKSSVDNGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFV3</accession>
    <name>A0A1A0HFV3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ARM repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_34511</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713520.1">
        <property type="nucleotide sequence ID" value="XM_018856479.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HFV3"/>
    <dbReference type="GeneID" id="30029455"/>
    <dbReference type="OrthoDB" id="294853at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032817">
        <property type="entry name" value="Mon2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16206">
        <property type="entry name" value="Mon2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mon2/Sec7/BIG1-like dimerisation and cyclophilin-binding" evidence="5">
        <location>
            <begin position="4"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2/Sec7/BIG1-like HUS" evidence="3">
        <location>
            <begin position="236"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Mon2 C-terminal" evidence="4">
        <location>
            <begin position="888"/>
            <end position="1031"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1554" mass="170644" checksum="DC0B69217269D759" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY0</accession>
    <name>A0A1A0HFY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioesterase/thiol ester dehydrase-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_35017</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713371.1">
        <property type="nucleotide sequence ID" value="XM_018856555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY0"/>
    <dbReference type="GeneID" id="30029531"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="4">
        <location>
            <begin position="38"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="5">
        <location>
            <begin position="172"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="39004" checksum="8A316F0B082E1975" modified="2016-10-05" version="1">MPGNAEPQNARDFEEATGVHQIDESTWEGNHPLRLPLTGARGVYGGHMCAQTLLVAMKTAPGYVPHSFHSHFIRAGNPKSKCIYKVSSLNDGKAFCQRQVTMLQDGKVTFTAMCSLKKKGVNFKSKVLGDYVHTESMFFKKYKDPSLLYKTYHTDFIVNAFSEEFLNYELCPDERNMQPSERWIHLWSKLHQPQKTKMSDPKLNYIGLAHLSDAAILTTMARALHLEWNPTADNPREEFDSEKDARKIMNVSLNAMHLFHYTAMSLDHHLYFHVDDTDELDVLNDWLFLTYQFMISRNSRTLVRGHFMNKDGVCVATFIQEGLTFMKPGVPGTTGKKFPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY4</accession>
    <name>A0A1A0HFY4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Topoisomerase 1-associated factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_34735</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the timeless family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713288.1">
        <property type="nucleotide sequence ID" value="XM_018856516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY4"/>
    <dbReference type="GeneID" id="30029492"/>
    <dbReference type="OrthoDB" id="310853at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044998">
        <property type="entry name" value="Timeless"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006906">
        <property type="entry name" value="Timeless_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940:SF4">
        <property type="entry name" value="PROTEIN TIMELESS HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22940">
        <property type="entry name" value="TIMEOUT/TIMELESS-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04821">
        <property type="entry name" value="TIMELESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Timeless N-terminal" evidence="11">
        <location>
            <begin position="102"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1072"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1227"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1264" mass="141910" checksum="A2DA6546276270A3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG33</accession>
    <name>A0A1A0HG33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP phosphoribosyltransferase</fullName>
            <ecNumber evidence="5">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_38195</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of the enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713426.1">
        <property type="nucleotide sequence ID" value="XM_018856969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG33"/>
    <dbReference type="STRING" id="869754.A0A1A0HG33"/>
    <dbReference type="GeneID" id="30029945"/>
    <dbReference type="OrthoDB" id="2574at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000003">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000123">
        <property type="entry name" value="HIS1p ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP-PRT_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF8">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP phosphoribosyltransferase catalytic" evidence="15">
        <location>
            <begin position="59"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine biosynthesis HisG C-terminal" evidence="16">
        <location>
            <begin position="224"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="304" mass="33020" checksum="606EBAB238FB9F79" modified="2016-10-05" version="1">MDLVNHLPDRLLFAVPKKGRLYEKCCNLLAGADIQFRRSNRLDIALSTNLPVALIFLPAADIPTFVGEGKCDLGITGLDQVREADMFESVSDLLDLEFGKCKLQVQVPADGDIEKPEQLVGKKIVSSFTRLSESFFKDLEVEAGVPVGSTKIKYVGGSVEASCALGVGDAIVDLVESGETMKAAGLKAISTVLDTSAHLISSKTPRFPQLVDTIHQRFEGIMAAQRFVLCNYNAPRSIVPEVLKITPGRRSATISALEKQPNDPEEWVAVSSMVEKKRIGNVMDNLKKAGASDILVFDISNCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG68</accession>
    <name>A0A1A0HG68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713334.1">
        <property type="nucleotide sequence ID" value="XM_018855400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG68"/>
    <dbReference type="STRING" id="869754.A0A1A0HG68"/>
    <dbReference type="GeneID" id="30028376"/>
    <dbReference type="OrthoDB" id="2538017at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="94" mass="10746" checksum="90BE21014AED94BF" modified="2016-10-05" version="1">MHLIIQLATPGTKLSSSFHLNEFFVKKIKKYRELLTVYENIVNESIHVTSGLEKTCNESNASIYSVVEVVKNQYSVFMELCEVVAQLHGEVERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGF1</accession>
    <name>A0A1A0HGF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PalH-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37156</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713725.1">
        <property type="nucleotide sequence ID" value="XM_018856831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGF1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGF1"/>
    <dbReference type="GeneID" id="30029807"/>
    <dbReference type="OrthoDB" id="4079240at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071467">
        <property type="term" value="P:cellular response to pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014844">
        <property type="entry name" value="PalH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALH/RIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35779:SF2">
        <property type="entry name" value="PROTEIN DFG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08733">
        <property type="entry name" value="PalH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="405"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="409"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="451"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="516"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="525" mass="58386" checksum="5365A5B1122EAEC7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGL7</accession>
    <name>A0A1A0HGL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vacuolar protein sorting 55</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OB-RGRP/VPS55 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713795.1">
        <property type="nucleotide sequence ID" value="XM_018858515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGL7"/>
    <dbReference type="GeneID" id="30031491"/>
    <dbReference type="OrthoDB" id="14246at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034424">
        <property type="term" value="C:Vps55/Vps68 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007262">
        <property type="entry name" value="Vps55/LEPROT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050">
        <property type="entry name" value="LEPTIN RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12050:SF0">
        <property type="entry name" value="RH04491P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04133">
        <property type="entry name" value="Vps55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="140" mass="15617" checksum="62332191A09A2FF5" modified="2016-10-05" version="1">MNPLNKIIFLLAFLAFGFLLILLSCALYNNWLPLWVIAIFLVAPLPNIVLSAIESNRDDFLTFSNDHGNAPTALEEFSKFFTGLLLVSGISLPLTFYHTNLIGLGSLVMSVLGGLIVYSDISVFIWFFSESEEANEDFNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGL9</accession>
    <name>A0A1A0HGL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_96140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713479.1">
        <property type="nucleotide sequence ID" value="XM_018859423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGL9"/>
    <dbReference type="GeneID" id="30032398"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="80" mass="9864" checksum="77D97D45529BB81B" modified="2016-10-05" version="1">MYACILCFFFIVSFDQFFPLMSRFIHSFYLFFVVYLFVRPIYFCNPVFRLIAWIYLQFDSDTLLSFFILLSFFLPLIFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGM7</accession>
    <name>A0A1A0HGM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phytanoyl-CoA dioxygenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29827</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713805.1">
        <property type="nucleotide sequence ID" value="XM_018855488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGM7"/>
    <dbReference type="STRING" id="869754.A0A1A0HGM7"/>
    <dbReference type="GeneID" id="30028464"/>
    <dbReference type="OrthoDB" id="2106152at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40470">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_2G15850)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40470:SF1">
        <property type="entry name" value="PHYTANOYL-COA DIOXYGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_2G15850)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32729" checksum="779323A9B922A559" modified="2016-10-05" version="1">MAVSHFHHDQIAEVGFTVIRGFLSQDEIGKYRAAAQRIVDHARAGKWKDVRTRGKQFPPWPENYSPDIWGVGGLMHPQLGDMSVPFQELYSDTKLLDVAGDILQTDNRGLALELFNMLINPLTDFELDWHRDSIKPEVSPQEEAEQLLADPYAGTQFNLALTDDKCLIVVPGSHKRLRTAEERDKTTDDSRKKPISGQITVDLRPGDIVFYNNNILHRAAYSSKAVRLTLHGSYGHRAHGKARARGVLQHGVGQWLDEFRPVNRNMGHLADGLKAVVEEFKGQDLGYAQDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGP0</accession>
    <name>A0A1A0HGP0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_29686</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713641.1">
        <property type="nucleotide sequence ID" value="XM_018855459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGP0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGP0"/>
    <dbReference type="GeneID" id="30028435"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="75"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="523" mass="58471" checksum="5F15DA308D9B2580" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS6</accession>
    <name>A0A1A0HGS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ARM repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_34334</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713676.1">
        <property type="nucleotide sequence ID" value="XM_018856465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS6"/>
    <dbReference type="GeneID" id="30029441"/>
    <dbReference type="OrthoDB" id="543373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041653">
        <property type="entry name" value="Importin_rep_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040928">
        <property type="entry name" value="Importin_rep_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041389">
        <property type="entry name" value="Importin_rep_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18808">
        <property type="entry name" value="Importin_rep_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18816">
        <property type="entry name" value="Importin_rep_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18829">
        <property type="entry name" value="Importin_rep_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291797" evidence="9">
        <location>
            <begin position="21"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="domain" description="IPO4/5-like TPR repeats" evidence="11">
        <location>
            <begin position="113"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="136"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Importin subunit beta-1/Transportin-1-like TPR repeats" evidence="10">
        <location>
            <begin position="462"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1084" mass="118171" checksum="36F9694C6DDAB89E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH47</accession>
    <name>A0A1A0HH47_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_104071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713801.1">
        <property type="nucleotide sequence ID" value="XM_018853762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH47"/>
    <dbReference type="STRING" id="869754.A0A1A0HH47"/>
    <dbReference type="GeneID" id="30026738"/>
    <dbReference type="OrthoDB" id="5584028at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852:SF1">
        <property type="entry name" value="HIG1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37852">
        <property type="entry name" value="YALI0B21208P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="177" mass="19437" checksum="7D359F5D25664C71" modified="2016-10-05" version="1">MEDLKPNQRLHLYPQERLQTVGAIAAMAGSAQGFFNGVKLSSLRYLTENAHRLPKTVGGWYFYHKKKNYIMLLAGFRQAATLAIKYSAGASAFLGLEAGLDYVRGTTDFLNTTAVGTVSSYIFGSANHMTRVQKWSFVKKGSLLALCYGMAQDALIYGRGGNVWYTKFGAGTSNIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHN2</accession>
    <name>A0A1A0HHN2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_29849</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. METL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713834.1">
        <property type="nucleotide sequence ID" value="XM_018855494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHN2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHN2"/>
    <dbReference type="GeneID" id="30028470"/>
    <dbReference type="OrthoDB" id="416496at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000371">
        <property type="entry name" value="Methyltransferase OMS1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF83">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransferase" evidence="11">
        <location>
            <begin position="271"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="464" mass="54750" checksum="35E57DAD0414B943" modified="2016-10-05" version="1">MLSRKILQNLRQCTFELTRGARGTAKINSILLRTPILSPFKRHYAQTPFPFDHKKAQEKRELEKLPKYKQWAKYFESMEFKRKMTKYYLATFGVMLVAFYFYMRDRYHEDKQMKHIKQKYAQDPASLSEYEYLKFKAFSTDKLKPREQKKFRLYQMMRKEFRRKNLFDASVMFEPTPQELDEWYSRQAKPTKKVALADDDKDVLFSNQLELEPEEPKVSNETNPNIQPAKDTTEFFDELASAYDDQVKWEERGIMMGSRRRWLMSQAQGDVLEVACGTGRNIPYLKTEQVDSITFMDSSSKMVEETQKKFRSKFPKYKNAAFAVGKAEDLVDLALDSFKYDTVIEAFGLCAHEDPVKALKNMATLLKPGGRIVLLEHGRSTWDFVNNHLDFRAEKRMHTWACRWNLDIGEMIDDAGLDITYEKRVHLGSTWLLVCKRPEDPFHIEEKPFINKLFGTEFTPVEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI14</accession>
    <name>A0A1A0HI14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 14">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="10 14">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_106293</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10 14">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10 14">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 14">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="10 14"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713964.1">
        <property type="nucleotide sequence ID" value="XM_018853814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI14"/>
    <dbReference type="STRING" id="869754.A0A1A0HI14"/>
    <dbReference type="GeneID" id="30026790"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.320:FF:000002">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000007">
        <property type="entry name" value="6-phosphogluconate dehydrogenase, decarboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006184">
        <property type="entry name" value="6PGdom_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00461">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="5 10 14" id="KW-0521">NADP</keyword>
    <keyword evidence="6 10 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10 14" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="15">
        <location>
            <begin position="195"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="26"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="12">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA23483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="507" mass="55523" checksum="FB18BF7B2305A461" modified="2016-10-05" version="1">MRAALRLAALRKHLTNNGSGDIGLIGLAVMGQNLILNAADKGFTVVAYNRTVAKVDEFLDNEAKGKSIIGAHSIEELVANLKRPRRIILLVKAGKPVDAFIEQLLPHLEKGDIIIDGGNSHYPDSNRRYEDLKEKGILFVGSGVSGGEEGARHGPSLMPGGHPDAWPHIKEIFQSIAAKTDGEPCCDWVGEAGAGHYVKMVHNGIEYGDMQLICEAYDLMKRVGKFSNKEIGDVFAKWNKGVLDSFLIEITRDIMYFNDPTDGQPLVEKILDTAGQKGTGKWTAINALDLGMPVTLIGEAVFSRCLSAIKGERERASAVLTGPTVEGESPITDKQKFVDDLEQALYASKIMSYAQGFMLIREAAKEYGWKLNNPAIALMWRGGCIIKSVFLGEVTAAYRETPDLENLLFHPFFQEAVTKAQQGWRSSIAKAIEFGVPVPAFTTALSFYDGYRSAQLPANLLQAQRDYFGAHTFKALPGKENDFLKGDWIHVNWTGRGGNISASTYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI29</accession>
    <name>A0A1A0HI29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial import receptor subunit TOM20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Mitochondrial 20 kDa outer membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial import receptor subunit tom20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Translocase of outer membrane 20 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_30213</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714300.1">
        <property type="nucleotide sequence ID" value="XM_018855574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI29"/>
    <dbReference type="STRING" id="869754.A0A1A0HI29"/>
    <dbReference type="GeneID" id="30028550"/>
    <dbReference type="OrthoDB" id="2154253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005742">
        <property type="term" value="C:mitochondrial outer membrane translocase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016031">
        <property type="term" value="P:tRNA import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.10:FF:000002">
        <property type="entry name" value="Mitochondrial import receptor subunit TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.10">
        <property type="entry name" value="Mitochondrial outer membrane translocase complex, subunit Tom20 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002056">
        <property type="entry name" value="MAS20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023392">
        <property type="entry name" value="Tom20_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12430:SF0">
        <property type="entry name" value="TRANSLOCASE OF OUTER MITOCHONDRIAL MEMBRANE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02064">
        <property type="entry name" value="MAS20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00351">
        <property type="entry name" value="OM20RECEPTOR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47157">
        <property type="entry name" value="Mitochondrial import receptor subunit Tom20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="223" mass="24335" checksum="1A276764C8469588" modified="2016-10-05" version="1">MRTRADIENFAGWSALLSAVANHCTSSEKRKTYHKMNRTLAFVSVAAAGVLAYAAYFDYNRRSSPEFRKNLKKRQVKQQKSAAKQQEELRKSLMLTVKKALTEDLAANPIPTDLSKKEEFFMEQVATGEQLANNPTTKVDAALRFYKALAVYPNPTDIMGIYQKTVPEDVYELLVMMIAIQPPATITNIIGGAAGVSGAAAAAEAAAAAVADEETKPSEVDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI63</accession>
    <name>A0A1A0HI63_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_36552</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714014.1">
        <property type="nucleotide sequence ID" value="XM_018856757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI63"/>
    <dbReference type="STRING" id="869754.A0A1A0HI63"/>
    <dbReference type="GeneID" id="30029733"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18577">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18578">
        <property type="entry name" value="ABC_6TM_Pgp_ABCB1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03228">
        <property type="entry name" value="ABCC_MRP_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394:SF1">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="661"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="705"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="783"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="809"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="886"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="924"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="23"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="356"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="665"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="986"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1231" mass="134019" checksum="DDB4D785E1776865" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI78</accession>
    <name>A0A1A0HI78_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_64822</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714191.1">
        <property type="nucleotide sequence ID" value="XM_018858349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI78"/>
    <dbReference type="GeneID" id="30031325"/>
    <dbReference type="OrthoDB" id="4077392at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="483" mass="55914" checksum="3AB5991A7BA600F8" modified="2016-10-05" version="1">MASIFHTIYESKDSTYSTRNFYAEYPSLEDIQIEPCQFKPTKLSESLATISNLCYDGFKDIDDMPQKLTKLRNTIMELMIYCIRIERSAYHEYIQALARKTNLFMERIVLKVLQACQDSESPASCFHLVKAIHDVVLLMYSFTNPLKIPDLLSGISSVSTEALVSAFKVEDIFEATKELHAFILCYPLLHDVDMEKPVPCSSSDNNTPKKLFKGEAAEILDYLAKINLVVINNTVPTASLFRYFESMLASDKFSLLELEYFEEFLSHFARVSKFCQLQQNTALFDRQKEELHSPFFQMIQLNPFNKLDWCTPLQAVNYLQIFVDLYNEIYNERLILERWYNNETGHEATVVRRIVDEQVAQACDCPQDHPSPATPEFVNFFSQSVIQQSESTPDTVAVESSVDYNGKLVHLRPPGGQLCGRLPNIQSPLPKSHKIFQLNEQVAYELRDFRSLIEERYRVNKVSFKEHKCLKCKYLPWHKHVYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HID1</accession>
    <name>A0A1A0HID1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SUN-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_9890</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714123.1">
        <property type="nucleotide sequence ID" value="XM_018859480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HID1"/>
    <dbReference type="STRING" id="869754.A0A1A0HID1"/>
    <dbReference type="GeneID" id="30032455"/>
    <dbReference type="OrthoDB" id="5339822at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051526">
        <property type="entry name" value="Beta-Glucosidase_SUN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005556">
        <property type="entry name" value="SUN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316">
        <property type="entry name" value="BETA-GLUCOSIDASE-LIKE PROTEIN NCA3, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31316:SF0">
        <property type="entry name" value="SECRETED BETA-GLUCOSIDASE SIM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03856">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0134">Cell wall</keyword>
    <keyword evidence="9" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291814" evidence="12">
        <location>
            <begin position="22"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="66"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="391" mass="40989" checksum="61134D164C434070" modified="2016-10-05" version="1">MRLEITTLYTLALAFASLAAAAPAKRDDEDCSTTQYHRHHQHRREVVIETEVITVTVDGELATTTSATTSKIPDAAETTQSPSTAKIDSESATLSTSTSTSVSSSSGSVSADDISGDLESYVDPTTKFVDGTIACSSFPGASGQGIINLDHLGFGGFSGIYNSDTSTGGSCEDGSYCSYACQLGMSKTQWPSEQPSNGVSVGGLLCKDGYLYRTNTDTDYLCEWGVDSAVVVSELDETVAICRTDYPGTENMVIPTVVDAGETSILTVVNQETYYTTNSGGKTSAQYYVNNAGVSWKDGCLWGTSGSGVGNWAPLNFGAGYDSGVSYLSLIKNPNNVDAANFNVKIEGESDATTVGSCSYEAGIYTSSSGDNTDGCTLSVTSGRGQFILYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIG9</accession>
    <name>A0A1A0HIG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Nucleoporin protein Ndc1-Nup</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_10026</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NDC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714286.1">
        <property type="nucleotide sequence ID" value="XM_018853673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIG9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIG9"/>
    <dbReference type="GeneID" id="30026649"/>
    <dbReference type="OrthoDB" id="67850at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070762">
        <property type="term" value="C:nuclear pore transmembrane ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106166">
        <property type="term" value="F:spindle pole body-nuclear membrane anchor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070631">
        <property type="term" value="P:spindle pole body localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019049">
        <property type="entry name" value="Nucleoporin_prot_Ndc1/Nup"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13269:SF6">
        <property type="entry name" value="NUCLEOPORIN NDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09531">
        <property type="entry name" value="Ndc1_Nup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="649" mass="72081" checksum="433139E97A4AAE98" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIJ9</accession>
    <name>A0A1A0HIJ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76649</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 8">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714149.1">
        <property type="nucleotide sequence ID" value="XM_018858566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIJ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIJ9"/>
    <dbReference type="GeneID" id="30031542"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="UniPathway" id="UPA00733"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036243">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006540">
        <property type="term" value="P:gamma-aminobutyrate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010102">
        <property type="entry name" value="Succ_semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01780">
        <property type="entry name" value="SSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="21"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="491" mass="53765" checksum="39527744D0611077" modified="2016-10-05" version="1">MAPVLKNKKLFEAKPFINGEWYNSKSERTFKVYDPATEEVIVELPDQTPEEIDEAINVTSKAFASYKNTSPTERSKWLRNLHNLMMENLDDLAAIITWENGKCLTDAIGEVKYAASYFEWFAEEAKRNYGHTIQPANFNNKVITYKQPVGPVGLLCPFNFPSAMGARKAAPAFAVGCTCVLKPDGQTPLSSLALAYLADQAGFPKGVFNVVLVSVESTPACGLKFCESPAIKKISFTGSTPVGKLLYKQCAPTLKKMSMELGGNAPVVVFNDARLDLAVDQAIASKFRSLGQTCVCANRLYVQSAVYDDFVSAFAEKVRQFKIGNGFEKGVTHGCLINTKTIDKVEEHVRDAVSKGAEIVVKGGTLQELGKHFYAPYVLKNITNDMKVVHEETFGPLAAIVKFETKEEVLKHCNDTPYGLAAYVFSESLNTIYTVSEALEVGMVSVNTGLFTDASMPFGGIKESGFGREGSLYGMDDYTVVKSITLGNVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIK5</accession>
    <name>A0A1A0HIK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein SYM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_37856</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714326.1">
        <property type="nucleotide sequence ID" value="XM_018856921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIK5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIK5"/>
    <dbReference type="GeneID" id="30029897"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="189" mass="20800" checksum="94B3596A7EF04255" modified="2016-10-05" version="1">MSFFFRKYNHLMNTRPLATNIATTGFLFGAGDFLAQQLEMRRPAPAGLAAKSAAYDMPRTARAVAYGSLVFGPLAGKWFGYLARVSVGSSAAANTLARVALDQCVFAPLVGIPLYFSCMTVMEAPADVRGAVTSKLHHAWWDTVRTNWMVWPAVQVANFGLVPPLFRLLVVNFLSIGWNCYLLMENSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIM5</accession>
    <name>A0A1A0HIM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Zf-U1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_35503</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714337.1">
        <property type="nucleotide sequence ID" value="XM_018856617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIM5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIM5"/>
    <dbReference type="GeneID" id="30029593"/>
    <dbReference type="OrthoDB" id="76567at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017340">
        <property type="entry name" value="U1_snRNP-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31148:SF1">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037969">
        <property type="entry name" value="U1_snRNP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="9">
        <location>
            <begin position="4"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="70"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="135" mass="15880" checksum="FB1DFA9A1DC837EF" modified="2016-10-05" version="1">MPKYYCDYCKSYLTHDKMSVRKSHLSGRNHIKFYCSYYEEKAKQLGIWDSSESKYQIDLEYLTSQEPSPDNFAREVVREREKSTVRRTDDMAEEVCLPPPPNLSKLAPPPPSVLRLTAEHSHLISVNMARQEYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIW1</accession>
    <name>A0A1A0HIW1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_10152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714431.1">
        <property type="nucleotide sequence ID" value="XM_018853696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIW1"/>
    <dbReference type="GeneID" id="30026672"/>
    <dbReference type="OrthoDB" id="4081078at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="277" mass="31167" checksum="13CA743996BC2319" modified="2016-10-05" version="1">MSATGSGGGNFRKRKLEKIKFCLTLLGNLVDDFQLRKSVPNNVVESSKVKQYLNRIEEGDSGQRPACIKMILCDSEADILTPAAQDLLRNDFNVESIYISEASSLSVDRIVTLHGRLSCVSRCALYISFMMNSGANNILKIEPFTLKSQNYQLCVLIEVDDQTIGAVFDDFTSGFIDWSQYERNSGLKIMKLRGDFLSLYKTLCSIFGRFLYQRYISDDRIVTLPVINLHDGDSLKVRSDKDNQKCDEENSNVLKLIYQTSYLKCSKPSVSNQGDGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ02</accession>
    <name>A0A1A0HJ02_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714299.1">
        <property type="nucleotide sequence ID" value="XM_018856701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ02"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ02"/>
    <dbReference type="GeneID" id="30029677"/>
    <dbReference type="OrthoDB" id="1913277at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="196" mass="21696" checksum="402A6CD1A7FBA810" modified="2016-10-05" version="1">MAESQPRVYPDSTNKWPTPFKNMLSSQTYPKWEPVDTAKQTKQVFKFGFFSVAGAYAWLYLWKRTTFKLELPMVVTGFATVAVAARGITANLREKNDGWNTFWAVAAGNAAVLTAGFKLVPLKHKLMTGISGACVSALAEQIMWAQSASSAGQHTRFAAANTDEELPKQEFWDVLKRRPMSQTVDALGVGRGIFKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJB7</accession>
    <name>A0A1A0HJB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DnaJ homolog 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76849</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714414.1">
        <property type="nucleotide sequence ID" value="XM_018858597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJB7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJB7"/>
    <dbReference type="GeneID" id="30031573"/>
    <dbReference type="OrthoDB" id="10256793at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006458">
        <property type="term" value="P:'de novo' protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000005">
        <property type="entry name" value="Chaperone protein dnaJ 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000002">
        <property type="entry name" value="Molecular chaperone DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01152">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012724">
        <property type="entry name" value="DnaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43096:SF52">
        <property type="entry name" value="DNAJ HOMOLOG 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="24"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="167"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="7">
        <location>
            <begin position="167"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="447" mass="48695" checksum="D1F711B5033BBF25" modified="2016-10-05" version="1">MLGTLKTQFTIAKASSQSRCIDFDPYDTLGVNKSASQSEIKKAYYQLVKKNHPDVNKEKNSEKKFHKIQESYELLSDKEKRAQYDQFGNAAFDANGNSNHFAGGNPFGGSQGGPFSGMGFDFEDLFKQAFNGGSQGRSGGRGQFVTEHVGDNIEVLKTISFKDAIFGTKVDVNYKAVDTCSSCSGSGLKKGKKKNTCRTCHGSGQSVHVMGGFQMSSTCNACSGSGVTIERKDQCSSCNGNGVEEIPKSSSVELPCGIADGSRLRIPGGGDAPFVTKDAYNQTRNGDLIVRVHVQKDLTFERVKNNIVLNHDILMTTAALGGEIVVPTIDGQKIKLKIRPGVQSGKKLTIPDKGVPINRNMANRGDMVVVLNVRTLTPETPVQTALLEALSDAFNDRNAKRMHGDLKNDIDELQHDEAIDENQLHPSKLKKMGQMLGKFFNLKDEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJC5</accession>
    <name>A0A1A0HJC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="3">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_18859</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714468.1">
        <property type="nucleotide sequence ID" value="XM_018854821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJC5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJC5"/>
    <dbReference type="GeneID" id="30027797"/>
    <dbReference type="OrthoDB" id="9995526at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01080">
        <property type="entry name" value="TruB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014780">
        <property type="entry name" value="tRNA_psdUridine_synth_TruB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767:SF2">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE TRUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13767">
        <property type="entry name" value="TRNA-PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase II N-terminal" evidence="6">
        <location>
            <begin position="66"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="38387" checksum="C993EE948A596EFF" modified="2016-10-05" version="1" fragment="single">MNGILAVEKPSGVSSSKVVLQIQSIFDGSDVFASDLAEMKQKVHDQLTLGTRWLEAKIANRVRRTKIKMGHGGTLDPLASGVLIIGVGSGTKQLSYYLGECTKTYETRAVLGQSTTTGDLEGEVVTQTDVAGVTLGRLQQAAAKFVGTIRQTPPVYSALKVNGKPLYEYAREGIPVPKAIEPRAVKVHLFVVHDDFGPHDKYGYLACPATADLMGKAVFNNPTLDDHELCYSAEFMARADVDDADKATNIRPQLVDPSHEPSGRPPVFHATASVGSGTYIRSLVSDLGRAVGLLAFMAELVRCKQANWELGKNVFQLSDFTERDQRVWGPVLKRVLDKGDSVVLADEFAAVEAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJE5</accession>
    <name>A0A1A0HJE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HIG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30473</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714616.1">
        <property type="nucleotide sequence ID" value="XM_018855645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJE5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJE5"/>
    <dbReference type="GeneID" id="30028621"/>
    <dbReference type="OrthoDB" id="1915122at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040153">
        <property type="entry name" value="Rcf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28018:SF3">
        <property type="entry name" value="RESPIRATORY SUPERCOMPLEX FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="6">
        <location>
            <begin position="88"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="207" mass="23545" checksum="2099DDFD3D555E6A" modified="2016-10-05" version="1">MKIIDREEKEAHAAHVMAEGFRGMFYGSIISAGLFAYLKTRHPARFAKFNTSIKACVLIMPTISLAAFYADDGSLEFDKKMYSAGHTNKQVLEQYREWKNMPASDRIVTLLSTHKYKIILASWAASMYGSWKLVNRDKIMTAPQKLVQARMYAQAITVVLLLATMVLAMKEEEVKSHQPAPLPEWRRVLIDREAAAMESQREEVTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJG6</accession>
    <name>A0A1A0HJG6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 15">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="11 15">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_10308</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11 15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 15">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11 15"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714622.1">
        <property type="nucleotide sequence ID" value="XM_018853733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJG6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJG6"/>
    <dbReference type="GeneID" id="30026709"/>
    <dbReference type="OrthoDB" id="361536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02658">
        <property type="entry name" value="Peptidase_C19B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14298">
        <property type="entry name" value="UBA2_scUBP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000086">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033864">
        <property type="entry name" value="UBA2_scUBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016652">
        <property type="entry name" value="Ubiquitinyl_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041432">
        <property type="entry name" value="UBP13_Znf-UBP_var"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF664">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17807">
        <property type="entry name" value="zf-UBP_var"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016308">
        <property type="entry name" value="UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11 15" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 11 15" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10 11 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="19">
        <location>
            <begin position="170"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="18">
        <location>
            <begin position="323"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="592"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="654"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="572"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="578"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="754"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016308-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA24141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="795" mass="86723" checksum="B7950029B32C9F3B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJR7</accession>
    <name>A0A1A0HJR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxin 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714539.1">
        <property type="nucleotide sequence ID" value="XM_018853721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJR7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJR7"/>
    <dbReference type="GeneID" id="30026697"/>
    <dbReference type="OrthoDB" id="5407351at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.230">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="384"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="48460" checksum="BA64781B79B3C717" modified="2016-10-05" version="1">MDASCGPANALSQLSKHTQRDNSLQHERQHLPGNLAPSAFRNGPGIDARLSQDFHRFNQGTSSDFQAHSLQNQNTYAPAALAPMGPRAHGGRNQEWVQDFRGLSLEEQSRAHPQSTGQKEGWHLQFMQNMQNMQNMQNMQNMPKTQNMQTMQSVSQNGWQNMQAHQPMMGLLGHQLHETQLGLQTHQHSDLHMSQIDDQALSRHFDVIEQELEQGENQAPQTHRAEQPGQAKQEHDNVQPVDDTEKQHFADAARQVHELMVTGHAQQSQETAHKFQQSDFLKLMSQILDRLVELSKEGDKLVQKSTGEDIRQHLSDPLRHERSHEPDYHVPPHGSVPADLANFPQTTNESQNIRSHLPDPLAHIRDGALDGDLTSFQAAQVVSGGQVQGGGWMEDDMWESEGRPAGRGRPSLMSQEDQEVYDDYRNDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GWL7</accession>
    <name>A0A1A0GWL7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_48173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000013">
        <property type="protein sequence ID" value="OBA16136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709242.1">
        <property type="nucleotide sequence ID" value="XM_018858274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GWL7"/>
    <dbReference type="STRING" id="869754.A0A1A0GWL7"/>
    <dbReference type="GeneID" id="30031250"/>
    <dbReference type="OrthoDB" id="3996251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34141:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="63" mass="6965" checksum="57F4CDC2CF2DC905" modified="2016-10-05" version="1" fragment="single">PTLRANPYPEVTDLFCRLPLSTLFYQLEAVNLGDLLRISVRLGMKVNPSCGFSRTVVGAPDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ49</accession>
    <name>A0A1A0GZ49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Class I glutamine amidotransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709300.1">
        <property type="nucleotide sequence ID" value="XM_018854467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ49"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ49"/>
    <dbReference type="GeneID" id="30027443"/>
    <dbReference type="OrthoDB" id="92161at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01741">
        <property type="entry name" value="GATase1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044992">
        <property type="entry name" value="ChyE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42695:SF5">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YLR126C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="1">
        <location>
            <begin position="112"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="292" mass="31907" checksum="52C02A87E8EAD14C" modified="2016-10-05" version="1">MEPHVAVLVLDTPIAGVAETFGDFGDNAISLLGRSKCGFVKYQVAFDVTMADRAEQNAQGCQRMLSALSEGIRDGAIKGAILTGSRTDSFLENHPWIAPLDKFIQTTLFNTPNFPMVGLCFGHQILAKNLGCKVNRNSPENGWEAGTTTISLNKSILDIPGSPFRSALKTEDGKILQHINLVEFHRDIVYGMPPASTHSLLSGTSFQNLGSTAKCSIQGLITESGPIKVLTFQGHPEFTTAESLNLLELMAQEQKIDKGVFERLTYNTRNLENQGPIVGKVIQEFIESHIQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H200</accession>
    <name>A0A1A0H200_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47842</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709375.1">
        <property type="nucleotide sequence ID" value="XM_018858229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H200"/>
    <dbReference type="STRING" id="869754.A0A1A0H200"/>
    <dbReference type="GeneID" id="30031205"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF7">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA17980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="405" mass="47484" checksum="4DDF52828858671F" modified="2016-10-05" version="1">MLSFLDGATAAILPGGSGSTVAPQNVDFKFVPRRNGDRNQILAHNQKVYDQVLAQEIHEPRDFDMDSIRPPQDPRDYSRENATIIALVRNNEVAGIKRTLRQFESNFNAKFQYPYTFLNDEPFTESFKERVRALTKADVQFVHIPPEVWNKPASIDLARERQAMDQMEKENVAYAKKGSYHNMCRFYSGLFHDLPELQKYKYYWRIEPNVRFYTDLNYDVFKYLAGTKKIYGFTISLYDIRQSVRTLLPETLRFLNQGDNYKYVDPNGAFQWITENQQNPHVTEATNGYSTCHFWSNFEIADMDFYRGDAYRNWFNHLEATGNFYYERWGDAPVHSLGLALFADKRKLHWFRDIGYYHMPYTNCPNNENTKGCTTGKFDDSGHNLDQNCMATWIEYEMESLGAIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4K7</accession>
    <name>A0A1A0H4K7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709544.1">
        <property type="nucleotide sequence ID" value="XM_018854423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4K7"/>
    <dbReference type="GeneID" id="30027399"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="108"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="310" mass="34057" checksum="78D3DA9DFA908D71" modified="2016-10-05" version="1">MKNVFGDSSDSEPELLAPPPKKFRPPTQNILCNESGLIGATKGQSIDEENASDSVSVKKVSDPSDLGSDSDFDNIDQELGLSRPGLGFQLKATPYNQKASLDHSIIDKTSIGYQIMEKMGYKQGQAIGMTNNPNALKEPLAIKSKLSRAGLGVSTFTPKRAADLSPESKSALQTASKNKHIERSNKAMILKLQKFCYAYSGEDMKLEDGTLFPEDVNPLWKDLAIRNSTPSCRTKLFGESFRARSSASEPNSPSSIKDVDTSQATEGQLMQLLQYTRVNFYYCPYCGTKFDDEEDLSSNCPGETEQLHQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4R3</accession>
    <name>A0A1A0H4R3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heat shock protein 70, Hsp70 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79921</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709475.1">
        <property type="nucleotide sequence ID" value="XM_018859149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4R3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4R3"/>
    <dbReference type="GeneID" id="30032125"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062040">
        <property type="term" value="C:fungal biofilm matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10233">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000001">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000028">
        <property type="entry name" value="heat shock 70 kDa protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000016">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="613"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="614"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="648" mass="70299" checksum="4D75F440BE6CD823" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X2</accession>
    <name>A0A1A0H4X2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA replication checkpoint mediator MRC1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33605</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709508.1">
        <property type="nucleotide sequence ID" value="XM_018856347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X2"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X2"/>
    <dbReference type="GeneID" id="30029323"/>
    <dbReference type="OrthoDB" id="2130597at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024146">
        <property type="entry name" value="Claspin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018564">
        <property type="entry name" value="Repl_chkpnt_MRC1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14396">
        <property type="entry name" value="CLASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14396:SF10">
        <property type="entry name" value="CLASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09444">
        <property type="entry name" value="MRC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication checkpoint mediator MRC1" evidence="6">
        <location>
            <begin position="711"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="279"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="413"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="656"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="715"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="856"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="954"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="375"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="681"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="435"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="738"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA18973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1071" mass="121495" checksum="3EC5684277E4FE8F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H547</accession>
    <name>A0A1A0H547_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_14156</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709610.1">
        <property type="nucleotide sequence ID" value="XM_018854483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H547"/>
    <dbReference type="STRING" id="869754.A0A1A0H547"/>
    <dbReference type="GeneID" id="30027459"/>
    <dbReference type="OrthoDB" id="9439903at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="118" mass="13559" checksum="992144146B0A551F" modified="2016-10-05" version="1" fragment="single">KDKARYTCSECNAAFKVKSYLTRHLRKHNNAKAFVCPFYREEDSEYCGTGKSGTKCHLTGGFSRKDTYKTHLKALHFIYPPRTKSSERGSQGGRCAGCFQYFESNSDWFKYHIEDGSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H549</accession>
    <name>A0A1A0H549_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="4">3.2.1.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Endo-1,3-beta-glucanase btgC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Laminarinase btgC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_47310</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation. Active on laminarin and lichenan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709583.1">
        <property type="nucleotide sequence ID" value="XM_018858147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H549"/>
    <dbReference type="STRING" id="869754.A0A1A0H549"/>
    <dbReference type="GeneID" id="30031123"/>
    <dbReference type="OrthoDB" id="68336at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF17">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BTGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00043078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="375" mass="43159" checksum="E03FF021208A4978" modified="2016-10-05" version="1">MSSRACTENEDKGESLRKISALSPTKFWIVILCLSLVISLYFAIFGSPISFRNFFNSLTDERKKELSNELRLKLVSTSESQLSLLNTPKKRKYLMGINYSPKGAYEPFCLFSKEDAIRDLSLISTVTLKIRTYGTQCNQMEHLLKAIDILRLDMKISMGIWLSNDEIRNKEQVDKAKEILLNSDTTIIQSIYIGNEALRRGDISEIAMISYIKDVKSYLDEKKIHIPVGISEFPSSVTQNLVDSCDIVGFTIQPFLAGIQSTESAKWSMNRFNALKTQFYNGKTHFAITEIGWPYQGGEFKRAKASPSDHKTFMHSWISNSAKSPTEDWFYYEAFNEPWKIRFNKEPDTWEAEWGLFDDQKRPRSDVQFVDGDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5T6</accession>
    <name>A0A1A0H5T6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMMECR1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709810.1">
        <property type="nucleotide sequence ID" value="XM_018858045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5T6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5T6"/>
    <dbReference type="GeneID" id="30031021"/>
    <dbReference type="OrthoDB" id="24630at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="208" mass="23179" checksum="76F2C478C5B98EF6" modified="2016-10-05" version="1">MSKSLALFAFESLVSELESSKRMTLAEVEKRTKSETALYPQNAPLFVTWNKDGNLRGCIGTFLNTPVEAGVAQYALISAFEDTRFAPIKFSELPSLSVGITLLDNFEEVLDPQDWVIGKHGLKVLFTDQGRRYLGTFLPLVAVEQEWTSNDTLWNLLRKSGSKSVSPAETTDFYASAIAAGTMRLVRYEGLKCSASYHEYKCAMSQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5W0</accession>
    <name>A0A1A0H5W0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">60S ribosomal protein L25</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_88614</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710002.1">
        <property type="nucleotide sequence ID" value="XM_018859293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5W0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5W0"/>
    <dbReference type="GeneID" id="30032268"/>
    <dbReference type="OrthoDB" id="1267328at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000035">
        <property type="entry name" value="60S ribosomal protein L23a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005633">
        <property type="entry name" value="Ribosomal_uL23_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03939">
        <property type="entry name" value="Ribosomal_L23eN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL23 N-terminal" evidence="9">
        <location>
            <begin position="13"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00075004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="16599" checksum="92FB756BCF3D37C7" modified="2016-10-05" version="1">MLITTSTLIATNTQAAAAKKSALKGTNSKKAVKVRTNTTFRLPKTLKLAKAPRYARKAVPHYNRMDAYKVIESPIASETAIKQVEDGNTLVFQVSLKSNKHQIKAAVKELYEVDVEKVNTMIRPNGTKKAYIRLTADHDALDVANRIGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6H5</accession>
    <name>A0A1A0H6H5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_45780</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710041.1">
        <property type="nucleotide sequence ID" value="XM_018857959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6H5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6H5"/>
    <dbReference type="GeneID" id="30030935"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032938">
        <property type="term" value="P:negative regulation of translation in response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="30"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="420" mass="45406" checksum="8F7560181205E241" modified="2016-10-05" version="1">MSSFFTQIAELPPDPLFGLKARFSADPRADKVDLGIGAYRDDAGKPWVLPAVRRAERKLIDSPAYNHEYLGISGHEPFLAEAAKVILGRLSRAIADGAVVSQQTLSGTGALHLAGAFLKQFYAGNRTVYLSQPTWANHHQIFSAVGLAVATYPYWDADAKALDMAGFMRTIETAPEGSVFVLHACAHNPTGLDPTPQQWAALLQALATHRHLPVFDSAYQGFASGNLDQDAAPIRQAIDLGVVAAPVLVCQSFAKNVGMYGERVGALHVVLPAGEPRAASDALKRAIKSQLNRYTRSEISNPPAYGAKVVATILADDALRAQWEQDLVTMSLRISKMRAALKARLLALGTPGNWDHITNQTGMFSFTGLTPDMVDRLEKVHGVYLVSSGRASVAGLNEGNVDRVAEAFHEVIQFHSKFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6J3</accession>
    <name>A0A1A0H6J3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Putative SWI/SNF family member, DNA-dependent ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_217110</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710108.1">
        <property type="nucleotide sequence ID" value="XM_018855096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6J3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6J3"/>
    <dbReference type="GeneID" id="30028072"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016817">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032392">
        <property type="term" value="P:DNA geometric change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000010">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000332">
        <property type="entry name" value="DNA repair and recombination protein RAD54-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013967">
        <property type="entry name" value="Rad54_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08658">
        <property type="entry name" value="Rad54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="284"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="614"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="853" mass="95805" checksum="35030CCDCCD24E41" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6T2</accession>
    <name>A0A1A0H6T2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP synthase subunit 5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_79161</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase delta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710329.1">
        <property type="nucleotide sequence ID" value="XM_018859012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6T2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6T2"/>
    <dbReference type="GeneID" id="30031988"/>
    <dbReference type="OrthoDB" id="1262810at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.520.20">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01416">
        <property type="entry name" value="ATP_synth_delta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026015">
        <property type="entry name" value="ATP_synth_OSCP/delta_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000711">
        <property type="entry name" value="ATPase_OSCP/dsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01145">
        <property type="entry name" value="ATP_synt_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11910">
        <property type="entry name" value="ATP SYNTHASE DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00213">
        <property type="entry name" value="OSCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00125">
        <property type="entry name" value="ATPASEDELTA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47928">
        <property type="entry name" value="N-terminal domain of the delta subunit of the F1F0-ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="22034" checksum="49DE6E4D3ED4E3A1" modified="2016-10-05" version="1">MMASRVFARSMASAAKSVKPPVQLFGVDGTYATALYSASVQESSVEASHKGLGKIAELVKQDPKVNEFLTNPALSKEDRKTVIDTLASSLKLDKTVTNFLTVLSENNRLGEFNNIYKNFGLLFDAHQGVVEATITSSKVLDSKILRKLQAAIQKSSFVGEGKTLKVSNTVNPEIMGGLIVDVGDKTVDLSIASKVAKLNSALSQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Z5</accession>
    <name>A0A1A0H6Z5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Defective in cullin neddylation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_13418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710205.1">
        <property type="nucleotide sequence ID" value="XM_018854358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Z5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Z5"/>
    <dbReference type="GeneID" id="30027334"/>
    <dbReference type="OrthoDB" id="27198at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031624">
        <property type="term" value="F:ubiquitin conjugating enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032182">
        <property type="term" value="F:ubiquitin-like protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14352">
        <property type="entry name" value="UBA_DCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.200">
        <property type="entry name" value="Cullin, PONY binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014764">
        <property type="entry name" value="DCN-prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042460">
        <property type="entry name" value="DCN1-like_PONY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005176">
        <property type="entry name" value="PONY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005637">
        <property type="entry name" value="TAP_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281:SF31">
        <property type="entry name" value="DCN1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12281">
        <property type="entry name" value="RP42 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03556">
        <property type="entry name" value="Cullin_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14555">
        <property type="entry name" value="UBA_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51229">
        <property type="entry name" value="DCUN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51281">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="TAP-C" evidence="4">
        <location>
            <begin position="68"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="DCUN1" evidence="3">
        <location>
            <begin position="117"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU410713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="328" mass="38055" checksum="FAFEA7D45318ED56" modified="2016-10-05" version="1">MCTGSVARSPALGPLNRKVVAGFNRYWTLIRTRKAYPGRLDHRSCLSIPYLKNPSIMIFHRTCTDHQPSRSELRKQFCNITRSSTEVATHFLENNQWDVSRAAHAFFDDASKTLDAIPSEALVHLFDKYKDPQNEAMLLIDGTLDYLNDLEIEPEDCRSLTLAFLLDSPQTGEFTRKSFLTFWALECITSLAEMRALILKCHSSIMKSPTEFEKFYQYCFNFIRGSDSRIKSITHEDAVSYWNLLFGECAYLEPCSERLKQWYEFVGSSKRPVSRDTWVMFYKFLIEVISKDPALLSGYDEMSSWPSMVDEFIEWLYEHGHLEAKSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H725</accession>
    <name>A0A1A0H725_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial phosphate carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_208587</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710361.1">
        <property type="nucleotide sequence ID" value="XM_018855017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H725"/>
    <dbReference type="STRING" id="869754.A0A1A0H725"/>
    <dbReference type="GeneID" id="30027993"/>
    <dbReference type="OrthoDB" id="427452at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990547">
        <property type="term" value="P:mitochondrial phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000024">
        <property type="entry name" value="MIR1p Mitochondrial phosphate carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044677">
        <property type="entry name" value="SLC25A3/Pic2/Mir1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671:SF12">
        <property type="entry name" value="MITOCHONDRIAL PHOSPHATE CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45671">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 (MITOCHONDRIAL CARRIER PHOSPHATE CARRIER), MEMBER 3, LIKE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="14"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="113"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="212"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="32162" checksum="D4B7F2D5DBE4C53A" modified="2016-10-05" version="1">MAEKARTIPSFTIGDYGAFALAGAMGCGITHGAMTPIDVIKTRIQLEPAVYNTGMVGSLRKIVSSEGAGALLTGLGPTILGYSMQGAFKFGGYELFKKTFIEQLGYDTAKNYKNSIYIGSAALAEFFADIALCPLEATRIRLVSQPNFANGLIGGFSRIMKEEGVGSFYNGFTPILFKQIPYNIAKFLVFERAAESIAAAVPGEKADFTSTTKTVVNLSAGIVAGCAAAIVSQPADTLLSKVNKTKKAPGQSTLGLLGQLAKQLGVRGSFAGLPTRLIMVGTLTSLQFTIYGSLKNALNCPPGVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H789</accession>
    <name>A0A1A0H789_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4484 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_44619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710287.1">
        <property type="nucleotide sequence ID" value="XM_018857816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H789"/>
    <dbReference type="GeneID" id="30030792"/>
    <dbReference type="OrthoDB" id="2152680at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028115">
        <property type="entry name" value="DUF4484"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018626">
        <property type="entry name" value="LCHN/Anr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053056">
        <property type="entry name" value="Lipid_Metab_Assoc_Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28153:SF1">
        <property type="entry name" value="DUF4484 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28153">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09804">
        <property type="entry name" value="DENND11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14831">
        <property type="entry name" value="DUF4484"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4484" evidence="2">
        <location>
            <begin position="468"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="521" mass="57739" checksum="4385FBEFAD18FA6A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D9</accession>
    <name>A0A1A0H7D9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_230387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710337.1">
        <property type="nucleotide sequence ID" value="XM_018855249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7D9"/>
    <dbReference type="GeneID" id="30028225"/>
    <dbReference type="OrthoDB" id="4096107at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013268">
        <property type="entry name" value="UTP16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08297">
        <property type="entry name" value="U3_snoRNA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="225" mass="25837" checksum="EC3A9437FF79A6FC" modified="2016-10-05" version="1">MVVTRSGKSVTTTGKKIKFLDDGGDPSDIEVVTVSSARESSQEPEQTGAASSGSDLDEDSDEAPEEESISQLKSTALQKKREQEKLEQSLRQQDREKRKQRDLQFKQQQEAKREKIKDLVLADQLPDLLPEEAFESEDEEDMLDNTRSGKHLRTEHFEQEDAAMRKRMKLEKLRRLKEMRNQALKKGPVYVQVHEGASKSRVPRAEQSVLDTKSQWLQRESLKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7E1</accession>
    <name>A0A1A0H7E1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Periplasmic binding protein-like II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_209746</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein SsuA/TauA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710420.1">
        <property type="nucleotide sequence ID" value="XM_018855025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7E1"/>
    <dbReference type="GeneID" id="30028001"/>
    <dbReference type="OrthoDB" id="1363at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13637">
        <property type="entry name" value="PBP2_Ca3427_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054364">
        <property type="entry name" value="Ca3427-like_PBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024:SF47">
        <property type="entry name" value="TAURINE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22384">
        <property type="entry name" value="PBP2_Ca3427_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Ca3427-like PBP 2" evidence="4">
        <location>
            <begin position="88"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="34668" checksum="E692ADEFEC78F7A1" modified="2016-10-05" version="1">MEKLVVAYIPEHFSTPMFLADEKGYYREEGLEIEFFPVIEGSGRLIKLLNDRTVDVAVGLTEAFVADISKGNKLYSIVGTYVESPLCWAISTGKNRDDIQSAADLAGSRIGVSRIGSGSYVMSFVLGLQNNFAAPYFSDFKVVHNFQNLRDSVNLKYRSDSGEQVDSEAFMWEHFTSKKYYDSGEIKRIGEIYTPWPSWVFTANQRVFETRKSNLSGFLRAVKKGVAYFLQNPEEAVDHISTHLNYSVDDAREWLTTVKFNEEIGVKPIDWHTVVENTAEVLKTAGVIPKDDIVGERLKMQVVSLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7V7</accession>
    <name>A0A1A0H7V7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_79297</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for 3'-end cleavage and polyadenylation of pre-mRNAs. Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710508.1">
        <property type="nucleotide sequence ID" value="XM_018859034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7V7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7V7"/>
    <dbReference type="GeneID" id="30032010"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180010">
        <property type="term" value="P:co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001039">
        <property type="entry name" value="Polyadenylation factor subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="124"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="165"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="207"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="351"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="462" mass="52220" checksum="5D6364B080784A90" modified="2016-10-05" version="1">MDQQLSLQEKKGQHRRTVDHGNNMGRWYIQRSIGQTKQHVGKVRPELSYLIDLLPSLAYSRDTLAGNPAILDIPSKFVHLSLNKARHSINTVKWTPEGRRLVVASHSGEFTIWNGMTFNFETIMQAHDLAVLALKYSHDDDWLLSGDQDGVIKYWQPNFNNVNILQGHTDAIRDIAFSPNDSKFLTCSDDHAIKIWNFNNGKEERTLLGHHWDVKCADWHPSLGLIVSGSKDNLIKLWDPRVATAINTLHGFKHTVTKTKFQRAGTNRLLASVSRDRSCKIFDLRTMTDFKVIRDHETDLSCVEWHPVHASLLTTAAYDGSMYHYILDRHIPETSSDKSQNSNVEIAQKVPYAHEKAINALEYHPLGHLLCSAGNDRSARFWSRARPNDPMAFKDPLYTNEKVGAWYYGVNNNVNAVLENPSLPTNSAQSAYGSRNIVPGLQNTYEDGSSYIPGLGSSGQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H817</accession>
    <name>A0A1A0H817_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase epsilon subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA polymerase II subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78689</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase epsilon subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710766.1">
        <property type="nucleotide sequence ID" value="XM_018858920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H817"/>
    <dbReference type="STRING" id="869754.A0A1A0H817"/>
    <dbReference type="GeneID" id="30031896"/>
    <dbReference type="OrthoDB" id="10254730at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045005">
        <property type="term" value="P:DNA-templated DNA replication maintenance of fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016266">
        <property type="entry name" value="POLE2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="8">
        <location>
            <begin position="331"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="618" mass="67572" checksum="83B3DE850E33B611" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8F8</accession>
    <name>A0A1A0H8F8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_197959</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710921.1">
        <property type="nucleotide sequence ID" value="XM_018854895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8F8"/>
    <dbReference type="STRING" id="869754.A0A1A0H8F8"/>
    <dbReference type="GeneID" id="30027871"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120112">
        <property type="term" value="P:UDP-glucose transmembrane transport into endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="289"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="38221" checksum="5F2B78705DBEEFA0" modified="2016-10-05" version="1">MKDSKFVTLVCCVLGLYALFLSWSLLQERLNTKPYAYVEEQAVFFKAPLAINATQALFAALVGLVYSRLAHAQNPFAVFASEPDAGGRAEIDYRYVRSFATIAITLSVSSPLGYLSLKHVDYLAFLLAKSCKLLPVMLVHLLLYKTKFPPHKYMVAALVTGGVVLFTLAKSGGKKAASFNDGNTLLGMAQLLALMFLDGFTNSTQDQLFRALGLKKKLTGATVMCVLNSFVFVLTTACLLLFKLDAEAKYVVSFVQQYPRALADMVAFALLGAVGQVFVFLILEKFDSLILVTATVTRKMISMILSVVLFGHHLVSWQWLGVLMVFGGIGYESFAKMQGRKTPKPKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8G5</accession>
    <name>A0A1A0H8G5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_45020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710702.1">
        <property type="nucleotide sequence ID" value="XM_018857859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8G5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8G5"/>
    <dbReference type="GeneID" id="30030835"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5016482906" description="Post-GPI attachment to proteins factor 3" evidence="7">
        <location>
            <begin position="24"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="396" mass="46935" checksum="34DEB30478B76816" modified="2016-10-05" version="1">MASSTSLASKALILLAISKLVISSPGDRLDEFEDCLFQCQQISCYHNPYHVMQEQYREPLQKRNYEFRRYEPSWKFDTELPQHLRFLLWDCKSNCDYQCQRIITRERKDNGNEVYQFHGKWPFVRVYGIQEFFSALFSVANFVPHFIGFRKVSQLRQRMPKERAALNPVLRNVQLLAFVTMCAWTCSTVFHIRDFLITERLDYYFAGLTVLTGLYAIGYRYLRLYLQSRQAYSWIWTALCMTAYVYHLRRLVTDWLYTYNMRANIVVGVLQNVVWGLTCFSLYSKFYKQEISGERAIDLTHLNYIQRGRIIFGSFFAKSAKLYSLYPLLLCGIVILGMSLEIFDFAPILGDLIDAHSLWHLVTVFPALLGWYDWMLWDIEENVWEELRTAQQKKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U4</accession>
    <name>A0A1A0H8U4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_43583</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710953.1">
        <property type="nucleotide sequence ID" value="XM_018857680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8U4"/>
    <dbReference type="GeneID" id="30030656"/>
    <dbReference type="OrthoDB" id="10254737at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10233:SF14">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="509" mass="57314" checksum="4EDC544A883DB68B" modified="2016-10-05" version="1">MSEEKTDKPEKQVKIEEPASEPGKISNKELKELKKKEKAAKRAAQKEAAGITPEQQEQMAQQKIEKKALQVTNTNLKKQLSQAVYKDDAKKIPVLFSHLETREQRNASSPTVSHIVHPAILTLSLNVASHKIVGSTARLREMLKVFKAVILDYKTPENTTLTRHLTGHLSHQIEYLKSARPLSLSMGNGIRWLKQEISLISIDTSEHDAKEILLQRIDDFIRERIELSDQLIIENSSHHITDGCTVLTFGHSEVLLQLFQHCVVEEGKSFNLVIVDLHPLFEGKKLLEGLVDTKIKAQEDEDPPTPHSRRTLPTPSITQEKLKITYVLINLLSSTVLEDVDLAFLGAHAMLSNGYLYSRVGTAMIAMMCNRRNISVLTCCESIKFSDRVQLDSVTTNELADPDDLLTNIDSKHPPQRKSMALEQFIKLKEELKKDDAPVRKQGNSDKKDAAADDSKLEPLKNWKSIAQLNILNIMYDLTPPHYINKVVTEFGALPPSSVPVILREYKNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Z5</accession>
    <name>A0A1A0H8Z5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ArfGap-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_12378</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710875.1">
        <property type="nucleotide sequence ID" value="XM_018854220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Z5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8Z5"/>
    <dbReference type="GeneID" id="30027196"/>
    <dbReference type="OrthoDB" id="983479at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030100">
        <property type="term" value="P:regulation of endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08830">
        <property type="entry name" value="ArfGap_ArfGap1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000014">
        <property type="entry name" value="ADP-ribosylation factor GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46395">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46395:SF1">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="8"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="315"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="190"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="349" mass="38261" checksum="A544D2744BB96270" modified="2016-10-05" version="1">MSIDPENRRKLLLLQKADDNKRCADCDAFNPQWALPKFGIFICLECAGIHRGLGVHISFVRSITMDQFKPEEVLRMEKGGNLNCRKYFEEHGLDAALPHRAKYDNPVAEDYKEYLSCLIEGRQFVPPDRSQQAAPPAQPNPGQNTLSQAPVVDKARTEAYFDRIRSQNETRPENVPPSQGGKYAGFGNTPAGQSRQGGASGASPGELSVNSLQQDPLGALTKGWSLFSSTVAKSVNEVHESVIKPGFTQLQGSELGSETKRAMAQFGQKMQQTGKYGQETFSTFTRDVQDKGFNESVGSFFGNIGGRTQAGSNVDRAFGFKKPDNEAKMPALGSDSLGGHKDEDEWDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H926</accession>
    <name>A0A1A0H926_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_199876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711027.1">
        <property type="nucleotide sequence ID" value="XM_018854919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H926"/>
    <dbReference type="GeneID" id="30027895"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="218" mass="23287" checksum="E1BE1C2825C28B92" modified="2016-10-05" version="1">MSIDTRGGARPVWAKVRQTKGRQKAGKWQANGRNMAQIWQKCQDYGKSTAGADMPLHNGDGWDNAGFCWSSQISSRHSATEPRKPQAGPAENLTAAYRPSSPGSVYRPPLVSSAGTRAGHVAVCLSGPATLGQRHWQATATKVEMVHVFNKRGHGHNVCILLPLPAGSHRCVTAPFQILASDSSAPIGPPSTASVGAVHVLEFGAPDFIWDHAAVRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9F2</accession>
    <name>A0A1A0H9F2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_43864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711271.1">
        <property type="nucleotide sequence ID" value="XM_018857707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9F2"/>
    <dbReference type="GeneID" id="30030683"/>
    <dbReference type="OrthoDB" id="3997968at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="271" mass="29885" checksum="B856A2CB8D059048" modified="2016-10-05" version="1">MVGHTTPRGVPQTPPSSTRKSFGNKPVPKFQLPDLQTPATTRKKPGLLTPDALAGPAHGTTLFPPSPDFTPRRSPRSKRKKLSFERILPPTESFNALLPNHGLVGSGRKHASTATAQPKLSGVFGLAEIFEINNNLDFEQDLPDSPSKRGRPRKQVPETPGKQLITEEKVHSWHGNAFRASFSSDEDESDAEATSIARLANPFLESKDAAADAPQLSRGHRQFNPFSSDKSAVDYSTHMELINHRTGERKVEVLLEEQRKFKPKKLNFSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9N2</accession>
    <name>A0A1A0H9N2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711222.1">
        <property type="nucleotide sequence ID" value="XM_018857767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9N2"/>
    <dbReference type="GeneID" id="30030743"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291747" evidence="1">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="67" mass="7260" checksum="0EDA8CDAFA51D484" modified="2016-10-05" version="1" fragment="single">KKIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPKVATGAKIIPMLMKYIVVPGTSRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9N7</accession>
    <name>A0A1A0H9N7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1711-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_43682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711227.1">
        <property type="nucleotide sequence ID" value="XM_018857686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9N7"/>
    <dbReference type="GeneID" id="30030662"/>
    <dbReference type="OrthoDB" id="4093188at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013175">
        <property type="entry name" value="INO80_su_Ies4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28061">
        <property type="entry name" value="INO EIGHTY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28061:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08193">
        <property type="entry name" value="INO80_Ies4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="15685" checksum="BEE83BA2C2560D52" modified="2016-10-05" version="1">MPSAKKYNVKVRLPTSYLETLPVFATPKPKSRAKKLSAEDKKNAGSPGSSPAPADDVPAARINTGPKELSTAGLTVNTVTQTLDKSGRPCRKWKKGPREFKTFTGFKVGLSVWKPEEDEAGVAHTEQTPVQESRAEESPSKHIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9P8</accession>
    <name>A0A1A0H9P8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sec1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_78939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711125.1">
        <property type="nucleotide sequence ID" value="XM_018858970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9P8"/>
    <dbReference type="STRING" id="869754.A0A1A0H9P8"/>
    <dbReference type="GeneID" id="30031946"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035543">
        <property type="term" value="P:positive regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="623" mass="69425" checksum="C5F3F25CB14EFC60" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA12</accession>
    <name>A0A1A0HA12_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711479.1">
        <property type="nucleotide sequence ID" value="XM_018857541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA12"/>
    <dbReference type="STRING" id="869754.A0A1A0HA12"/>
    <dbReference type="GeneID" id="30030517"/>
    <dbReference type="OrthoDB" id="5865767at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37283:SF1">
        <property type="entry name" value="PH DOMAIN-CONTAINING PROTEIN YHR131C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="443" mass="49902" checksum="F7697ED909756F0D" modified="2016-10-05" version="1">MQRVETPFVPGNPPIEGLPPYKDDIPAYTPSLQFYGIALLKMEFDTPWSDRSTLLKPVVVELNSNQLNMYEFAADKTTVGVVGSLFKHQNYADASQEPPAADYTELYFFDCDAYGDSSAPEAPKPMFSKLRNCLDKKKTQRVLSASLSPGMCLNKLLLEPTSDRAVYDQFAAIYRGKLIHSYTLAHLNVGEAPCLTLARYKEEKRHSANAMTLVNYRNTLRLRIEYTQLLLHFWSFHGMVHWFRNMSVGRDLASSIDDRKLTVLKSVPRNFSVANNALLEASAREALTFNPDTCKMVHRDSISSAEDRASCFSEYTMGSGESGASSVASACSESCKNLTVDIYGAKLTCFENMYTPIEKQYISKCIPVLNSFDKWMGAKITISNYEHFVPRLTRDNCNENENVFISHYKFSDLVKTYNRNPAATSGPRRCRDFYVDSAGLVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAA7</accession>
    <name>A0A1A0HAA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L domain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711330.1">
        <property type="nucleotide sequence ID" value="XM_018857720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAA7"/>
    <dbReference type="STRING" id="869754.A0A1A0HAA7"/>
    <dbReference type="GeneID" id="30030696"/>
    <dbReference type="OrthoDB" id="1394818at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019487">
        <property type="entry name" value="RAM_signalling_pathway_SOG2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366">
        <property type="entry name" value="IG(IMMUNOGLOBULIN) AND LRR(LEUCINE RICH REPEAT) DOMAINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24366:SF96">
        <property type="entry name" value="LEUCINE RICH REPEAT CONTAINING 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10428">
        <property type="entry name" value="SOG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="170"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="257"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="308"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="595"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="848"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="343"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="906" mass="97364" checksum="0B5670F4CAB36504" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAF5</accession>
    <name>A0A1A0HAF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Large ribosomal subunit protein mL59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711495.1">
        <property type="nucleotide sequence ID" value="XM_018857333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAF5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAF5"/>
    <dbReference type="GeneID" id="30030309"/>
    <dbReference type="OrthoDB" id="18529at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037507">
        <property type="entry name" value="Ribosomal_mL59"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040922">
        <property type="entry name" value="Ribosomal_mL59_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28041:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18126">
        <property type="entry name" value="Mitoc_mL59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein mL59" evidence="1">
        <location>
            <begin position="14"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="157" mass="18849" checksum="0301E050F4CCA5E2" modified="2016-10-05" version="1">MSLTPKEAFAKLPQKLHKFFIRYPPRPFASYLDKPSTIKDPALNPFLPNKNSETGRWHSAKVSLRRLADLYKMARKYGIEDLLPPVPHRKFHEDKYENKNWMRGVLFQKKQKWERELPAKLERRRVALENMDETILAARPSYKKQLAKREQNKRTWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG0</accession>
    <name>A0A1A0HAG0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_40756</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711500.1">
        <property type="nucleotide sequence ID" value="XM_018857307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAG0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAG0"/>
    <dbReference type="GeneID" id="30030283"/>
    <dbReference type="OrthoDB" id="1740265at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033934">
        <property type="term" value="F:glucan 1,4-alpha-maltotriohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000025">
        <property type="term" value="P:maltose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11333">
        <property type="entry name" value="AmyAc_SI_OligoGlu_DGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000064">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.400.10:FF:000004">
        <property type="entry name" value="Oligo-1,6-glucosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000087">
        <property type="entry name" value="Oligo-1,6-glucosidase IMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.400.10">
        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF179">
        <property type="entry name" value="NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0462">Maltose metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="5">
        <location>
            <begin position="15"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="563" mass="65263" checksum="AB612A7453A8939B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAH8</accession>
    <name>A0A1A0HAH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleoporin Nup54 alpha-helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_78191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711644.1">
        <property type="nucleotide sequence ID" value="XM_018858842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAH8"/>
    <dbReference type="STRING" id="869754.A0A1A0HAH8"/>
    <dbReference type="GeneID" id="30031818"/>
    <dbReference type="OrthoDB" id="6162375at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006607">
        <property type="term" value="P:NLS-bearing protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006999">
        <property type="term" value="P:nuclear pore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024864">
        <property type="entry name" value="Nup54/Nup57/Nup44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025712">
        <property type="entry name" value="Nup54_alpha-helical_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13000:SF0">
        <property type="entry name" value="NUCLEOPORIN P54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13874">
        <property type="entry name" value="Nup54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup54 alpha-helical" evidence="7">
        <location>
            <begin position="342"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="100"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="166"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="219"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="560" mass="57206" checksum="90FA764F3D70BE12" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAL5</accession>
    <name>A0A1A0HAL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_167125</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkylbase DNA glycosidase AlkA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711427.1">
        <property type="nucleotide sequence ID" value="XM_018854667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAL5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAL5"/>
    <dbReference type="GeneID" id="30027643"/>
    <dbReference type="OrthoDB" id="415889at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032993">
        <property type="term" value="C:protein-DNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032131">
        <property type="term" value="F:alkylated DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA alkylation repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051912">
        <property type="entry name" value="Alkylbase_DNA_Glycosylase/TA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000035">
        <property type="entry name" value="Alkylbase_DNA_glycsylse_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43003:SF5">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00516">
        <property type="entry name" value="ALKYLBASE_DNA_GLYCOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="5">
        <location>
            <begin position="163"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="42218" checksum="6C186E5FAEED5072" modified="2016-10-05" version="1">MRVTRSRSAGKPEDLGLQGLAKVGKPQRKTEQLRRFPATQESLVSSAVKSEGHPGLSDSAPVDQASLVQVPRLPSKTSAKPHIEQSFAHIQTPADPELPPNFVTYHTPEFVSAIKHILNIDSTLYPAIAHGNFAAFANKNEDSQLEGLELINSYWYSLISSVISQQVSGSAAKAIQTRFNDLFEGTPTPKATLDLQLDTLKAVGLLSMKLKYVVHISEVFSDPESRLTSVEFYKHTSTEELIAELTKLKGIGEWSANMFAVFTLKELDIFAYDDLGVARGVARYLQVRPKTLEETKNGVHAIEELKARLKRKSKFHSAKSKRDWTPLHDEYVKFLALKFEPYRTAFMLVMWRLSSTNIDVLEKDDELRDTGSTASRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAS2</accession>
    <name>A0A1A0HAS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41529</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PIR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711729.1">
        <property type="nucleotide sequence ID" value="XM_018857415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAS2"/>
    <dbReference type="GeneID" id="30030391"/>
    <dbReference type="OrthoDB" id="4073697at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="6">
        <location>
            <begin position="29"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="113" mass="12709" checksum="038FDFDA3D805A52" modified="2016-10-05" version="1">MKKKSRAGPSIIPLACLSESVLELDLSDGLLTSRQHNVASVDDTHQFQFEELYDSAKYTPRAWLVSAKGQLKYQDTELFYQCHSGESYKIYDAPVHSRCAPVLLDVVELVSCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAV2</accession>
    <name>A0A1A0HAV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_178628</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711759.1">
        <property type="nucleotide sequence ID" value="XM_018854740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAV2"/>
    <dbReference type="STRING" id="869754.A0A1A0HAV2"/>
    <dbReference type="GeneID" id="30027716"/>
    <dbReference type="OrthoDB" id="284718at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045048">
        <property type="term" value="P:protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005351">
        <property type="entry name" value="ASTER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28038">
        <property type="entry name" value="ADL329WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28038:SF1">
        <property type="entry name" value="ADL329WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03669">
        <property type="entry name" value="ASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="116" mass="12484" checksum="885579BB4F5FC108" modified="2016-10-05" version="1">MSSPKRADLVVPYTHKPAKPRSEASSMISQTLPMAAMFMRNKLLSWSSVFLALQSYLTEPVNRPAGSDGGSQPPLLRLAFAALSLATCYLEYFFPSTSPTLKRAAQAASLTATSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAV5</accession>
    <name>A0A1A0HAV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytosine deaminase</fullName>
            <ecNumber evidence="14">3.5.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Cytosine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_42736</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the hydrolytic deamination of cytosine to uracil or 5-methylcytosine to thymine. Is involved in the pyrimidine salvage pathway, which allows the cell to utilize cytosine for pyrimidine nucleotide synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>cytosine + H2O + H(+) = uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; uracil from cytosine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.1" evidence="14"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711764.1">
        <property type="nucleotide sequence ID" value="XM_018857579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAV5"/>
    <dbReference type="GeneID" id="30030555"/>
    <dbReference type="OrthoDB" id="408702at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004131">
        <property type="term" value="F:cytosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046087">
        <property type="term" value="P:cytidine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019858">
        <property type="term" value="P:cytosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000016">
        <property type="entry name" value="Cytosine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF190">
        <property type="entry name" value="CYTOSINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="17">
        <location>
            <begin position="3"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00084039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="16410" checksum="0A20117C22DC3C4B" modified="2016-10-05" version="1">MPFDDKLGMQIALEEARKSLAEGGIPIGGALIHEDGRVLGRGHNMRIQKNSATLHGEISTLENAGRLPGALYKDCTMYTTLSPCHMCSGACIMYGVKRVVLGENVNFVGAEELVKSKGIEVVNLDDPECKKIMSDFIKARPEDWFEDIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB01</accession>
    <name>A0A1A0HB01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-ubiquinone oxidoreductase 78 kDa subunit, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_78307</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711814.1">
        <property type="nucleotide sequence ID" value="XM_018858861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB01"/>
    <dbReference type="STRING" id="869754.A0A1A0HB01"/>
    <dbReference type="GeneID" id="30031837"/>
    <dbReference type="OrthoDB" id="10249365at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02773">
        <property type="entry name" value="MopB_Res-Cmplx1_Nad11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.740:FF:000016">
        <property type="entry name" value="NADH dehydrogenase (Quinone), G subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.740:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.210:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.20:FF:000002">
        <property type="entry name" value="NADH-ubiquinone oxidoreductase 75 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054351">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015405">
        <property type="entry name" value="NDUFS1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050123">
        <property type="entry name" value="Prok_molybdopt-oxidoreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105:SF13">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43105">
        <property type="entry name" value="RESPIRATORY NITRATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13510">
        <property type="entry name" value="Fer2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22151">
        <property type="entry name" value="Fer4_NDSU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22117">
        <property type="entry name" value="Fer4_Nqo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09326">
        <property type="entry name" value="NADH_dhqG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53706">
        <property type="entry name" value="Formate dehydrogenase/DMSO reductase, domains 1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="12">
        <location>
            <begin position="2"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="14">
        <location>
            <begin position="80"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="219"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="699" mass="75682" checksum="AC8C934059D4B542" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB73</accession>
    <name>A0A1A0HB73_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Mitochondrial DNA polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_78284</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711771.1">
        <property type="nucleotide sequence ID" value="XM_018858855.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HB73"/>
    <dbReference type="GeneID" id="30031831"/>
    <dbReference type="OrthoDB" id="5588663at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005760">
        <property type="term" value="C:gamma DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032043">
        <property type="term" value="P:mitochondrial DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002297">
        <property type="entry name" value="DNA-dir_DNA_pol_A_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041336">
        <property type="entry name" value="DNApol_Exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10267:SF0">
        <property type="entry name" value="DNA POLYMERASE SUBUNIT GAMMA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18136">
        <property type="entry name" value="DNApol_Exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00867">
        <property type="entry name" value="DNAPOLG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family A palm" evidence="7">
        <location>
            <begin position="687"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1247"/>
            <end position="1284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1284" mass="146435" checksum="E4EDBF60AF4FF9D3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBA3</accession>
    <name>A0A1A0HBA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78301</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711801.1">
        <property type="nucleotide sequence ID" value="XM_018858859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBA3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBA3"/>
    <dbReference type="GeneID" id="30031835"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000010">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-16 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="147" mass="16394" checksum="BDA07651BB616E50" modified="2016-10-05" version="1">MSLKRINKELLDLGRDPPSSCSAGPVGDDLYHWQASIMGPPDSPYAGGVFFLSIHFPTDYPFKPPKVNFTTKIYHPNINGSGSICLDILKDQWSPALTISKVLLSICSLLTDANPDDPLVPEIAHVYKVDRQKYETTAREWTKKYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBH3</accession>
    <name>A0A1A0HBH3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_42731</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711871.1">
        <property type="nucleotide sequence ID" value="XM_018857578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBH3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBH3"/>
    <dbReference type="GeneID" id="30030554"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF10">
        <property type="entry name" value="MANNOSYLTRANSFERASE KTR2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="378" mass="44898" checksum="B0562B16DC7F586B" modified="2016-10-05" version="1">MDAFVAKYWYNPALAGQQKLSSNSYFDVLSTQRDRRQIGRENATLVMLVRNSELKGALQSMRSLEDRFNRDYKYPWVFLNDVPFDEDFMDQTTAMASGETFYELVPPEDWNPPPHIDRRRLDDNIANSQHIIYGFSKSYRNMCHFNSGYFYRQKRLLDYEWYFRVEPDVEYMCDFMYDPFELLRTNNKTYGFTITIPEYQDTIPTLWDTVEDFISKFPQHLHPNNAYDFLTTNDSDVFFHTHAHSDSKYNMCHFWSNFEVGNLDFFRGEAYGAYFDHLDQAGGFYYERWGDAPVHSIGLALLLDRDAIHHFEDIGYYHAPYMACPQSREVQSVKRCICRKFDENGEHKGVDVMPPSCLPRWWRYGGGKKFLNENDYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBW3</accession>
    <name>A0A1A0HBW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_40795</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the histone H2A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712011.1">
        <property type="nucleotide sequence ID" value="XM_018857314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBW3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBW3"/>
    <dbReference type="GeneID" id="30030290"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000008">
        <property type="entry name" value="Histone H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="12 13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="15">
        <location>
            <begin position="9"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="16">
        <location>
            <begin position="92"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA21501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="130" mass="13805" checksum="7DEE39F31BC2A5C5" modified="2016-10-05" version="1">MSGGKGKAGSTEKASTSRSSKAGLTFPVGRVHRLLRKGNYAQRVGSGAPVYLTSVLEYLAAEILELAGNAARDNKKSRIIPRHLQLAIRNDEELNKLLGHVTIAQGGVLPNIHQSLLPAKKAAAKASQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBZ7</accession>
    <name>A0A1A0HBZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA polymerase I-specific transcription initiation factor RRN3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_41530</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711923.1">
        <property type="nucleotide sequence ID" value="XM_018857416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBZ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HBZ7"/>
    <dbReference type="GeneID" id="30030392"/>
    <dbReference type="OrthoDB" id="26970at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000182">
        <property type="term" value="F:rDNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001181">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007991">
        <property type="entry name" value="RNA_pol_I_trans_ini_fac_RRN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790:SF0">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12790">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IA RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05327">
        <property type="entry name" value="RRN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="586"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="605"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="653"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="666"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="678" mass="77493" checksum="CBC81BB1246E3CA5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC27</accession>
    <name>A0A1A0HC27_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_11958</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711953.1">
        <property type="nucleotide sequence ID" value="XM_018854131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC27"/>
    <dbReference type="GeneID" id="30027107"/>
    <dbReference type="OrthoDB" id="10266999at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07440">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RGS" evidence="2">
        <location>
            <begin position="30"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="273" mass="30958" checksum="A260DD3C6DC7B576" modified="2016-10-05" version="1">MPPVHFRLPGVVPSLDEIVCSEPCALQPGPYSKAAFVDFLRLSHCAENLDFVWDVDRYLERFCQQEAVPFLDDETILENLRLVRLWTLIYDTYICRTAPREVNVPGKLLARFLAHVLPDHKEVLVLRKAVYELLSANYNDFVTSAREHHAHHILCRRPLEAVAPEALLQDHDSPHDVLSPDTIFHIISPDLLVQWEKTLETYEMLKLASSCESAGLPTRNNSAGAASTRPSSRGSSLGSVFDNIREYSGWKKTVRKLRPRRSSSEKPADDMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC74</accession>
    <name>A0A1A0HC74_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein transport protein SEC61 alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_11984</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Appears to play a crucial role in the insertion of secretory and membrane polypeptides into the ER. It is required for assembly of membrane and secretory proteins and is essential for cell growth. It interacts with other membrane proteins required for protein translocation. Upon binding to SEC62/63 complex, secretory precursor polypeptides may engage SEC61 to begin membrane penetration event. A cycle of assembly and disassembly of SEC62/63 from SEC61 may govern the activity of the translocase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotrimeric complex composed of SEC61-alpha, SEC61-beta and SEC61-gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711987.1">
        <property type="nucleotide sequence ID" value="XM_018854135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC74"/>
    <dbReference type="STRING" id="869754.A0A1A0HC74"/>
    <dbReference type="GeneID" id="30027111"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070843">
        <property type="term" value="P:misfolded protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000002">
        <property type="entry name" value="Transport Sec61 subunit alpha isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 12 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="440"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="15">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="478" mass="52045" checksum="FA573B34B2E4A14B" modified="2016-10-05" version="1">MSFRVLDLVKPFEPLVPEVIAPERKVPFNQRVMWTGVTLLIFLVMSEVPLYGIASSDGSDPLLWLRMMLALNRGTLMELGITPIVSASMVFQLLLGTKLIHVDMTKKEDREQFQTAQKLFAIMLAVGQATVYVLTGMYGPPRTLGTGVCLLLVLQLVFAGIIVILLDELLQKGYGLGSGISLFTATNVCELVMWKAFAPTTSASGKGAEFDGAVVALFHLLGSRKDKKRALLEAFYRQNLPNMLQVLATVLVFFAVVYLQGVRVDLPVTSVRQRGAYIMYPIRLFYTSNTPIMLQSALSSNIFLISQMLFMRWPNNLFVKLLGSWGARGGSSQLYATSGLSYYIQPPTSFAEALVDPVKTVIYVTFVLGSCAVFSTTWIEISGTSPRDVAKQFKDQGLVIAGYRSTSAYKELKRIIPTAAALGGVAIGALSVISDMVGCLGSGTSILLSVTTIYGYYELAVKEGGFSKSVVSGYVDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCB1</accession>
    <name>A0A1A0HCB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Succinate dehydrogenase [ubiquinone] cytochrome b small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_42429</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CybS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712141.1">
        <property type="nucleotide sequence ID" value="XM_018857543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCB1"/>
    <dbReference type="STRING" id="869754.A0A1A0HCB1"/>
    <dbReference type="GeneID" id="30030519"/>
    <dbReference type="OrthoDB" id="18577at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03496">
        <property type="entry name" value="SQR_TypeC_CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase, transmembrane subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007992">
        <property type="entry name" value="CybS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13337:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] CYTOCHROME B SMALL SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05328">
        <property type="entry name" value="CybS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="Fumarate reductase respiratory complex transmembrane subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <note>ligand shared with SDHC</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <note>ligand shared with IP/SDHB</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR607992-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="146" mass="15620" checksum="A750B4706718D0E3" modified="2016-10-05" version="1">MPASAPFLKASLARGFKTIPQPPGNIVGTVNDAYVPPKAHKTHGSWHWTSERIVAAGLIPLVATSFTSGTSVMLDTTLSTLMLYHCYAGMQSCIIDYIPKRVYGALHSAAMYLLLLGTGVAGYGIYDIEQKEEGGVAGIIARVWHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG0</accession>
    <name>A0A1A0HCG0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_39216</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712295.1">
        <property type="nucleotide sequence ID" value="XM_018857116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCG0"/>
    <dbReference type="GeneID" id="30030092"/>
    <dbReference type="OrthoDB" id="5121955at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052073">
        <property type="entry name" value="Amide_Lactam_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47171:SF3">
        <property type="entry name" value="FARA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="30"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="66"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="646"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="702"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="671"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="710"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="843" mass="94122" checksum="503D10521AB06BB8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG5</accession>
    <name>A0A1A0HCG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL13m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_41201</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712196.1">
        <property type="nucleotide sequence ID" value="XM_018857368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCG5"/>
    <dbReference type="GeneID" id="30030344"/>
    <dbReference type="OrthoDB" id="274622at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1180.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="173" mass="19649" checksum="59A70CB27871965A" modified="2016-10-05" version="1">MSQRIGNAAAAFTRAWHHVDVAADSRTMGRLAEGIARTLQGKHKPTYSPNRDDGDYVVVTNCSHLKTTGRKLEQKMYWSHTTRPGSLKLVPMERMIANKGYGEVLMRAVKGMIPKNKLRLDRLDRLKIFDGDEHPYKENLIAFADEGPDVRAKKEALEQRTARIAELRTKFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCV6</accession>
    <name>A0A1A0HCV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="7 19">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_12231</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 20">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="7 19"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712268.1">
        <property type="nucleotide sequence ID" value="XM_018854195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCV6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCV6"/>
    <dbReference type="GeneID" id="30027171"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00318">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000019">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000031">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF0">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="12 19 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="372"/>
            <end position="375"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA21758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="417" mass="44690" checksum="632B943744A47BAC" modified="2016-10-05" version="1">MSLSNKLSVKDLELANKRVFIRVDFNVPLDGTTITNNQRIVAALPTIKYVLEQKPKAVVLASHLGRPNGERVEKYSLAPVAKELQSLLSDQKVTFLNDCVGTDVENAVNGASAGEVFLLENLRYHIEEEGSRKVDGNKEKASAEDVQKFRQGLTALADVYVNDAFGTAHRAHSSMVGLDLPQKAAGFLMAKELEYFAKALENPIRPFLAILGGAKVSDKIQLIDNLLDKVDILIVGGGMSFTFKKVLENMPIGTSLFDEAGAKNVEHLVAKAKKNNVEIVLPVDFVTADDFNKDANTGVATQEEGIPDGWMGLDAGPKSRKLFDEAVAKAKTIVWNGPPGVFEFEKFSQGTKSLLDAAVKSAEAGNTVIIGGGDTATVAKKFGVVEKLSHVSTGGGASLELLEGKELPGVVAISDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCZ2</accession>
    <name>A0A1A0HCZ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FSH1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_39670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712341.1">
        <property type="nucleotide sequence ID" value="XM_018857167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCZ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCZ2"/>
    <dbReference type="GeneID" id="30030143"/>
    <dbReference type="OrthoDB" id="2094269at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005645">
        <property type="entry name" value="FSH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050593">
        <property type="entry name" value="LovG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070">
        <property type="entry name" value="ESTERASE OVCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48070:SF9">
        <property type="entry name" value="FAMILY OF SERINE HYDROLASES 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03959">
        <property type="entry name" value="FSH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine hydrolase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="255" mass="28666" checksum="AF192D7D2A16F63C" modified="2016-10-05" version="1">MTKILCLPGFLQSGAVFAEKSSGLRKLLTKKLRYDLDYIDPPVVISSKEQLPFKLSEDPLEESEKWARILEKGLNRCWWLHTSDGEYEGFDEAMKFVTAYIRQNGPYDGIIGFSQGAAMAAVIANSIEKSVPQNGPLAIAVMFSPFAFTVPRKSRDVMARLNSEVTDVREYANLVSLNPLYREYFTQQLSGTKTVAVYGMVDTVVPPVRTEYLCSLYAQIDIMKHDGGHYMPNKKLFLDPIVELFKKACESTPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD07</accession>
    <name>A0A1A0HD07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_10783</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712356.1">
        <property type="nucleotide sequence ID" value="XM_018853855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD07"/>
    <dbReference type="STRING" id="869754.A0A1A0HD07"/>
    <dbReference type="GeneID" id="30026831"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF88">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="151"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="826" mass="91010" checksum="761E87BD45FF5012" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD25</accession>
    <name>A0A1A0HD25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_77354</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712278.1">
        <property type="nucleotide sequence ID" value="XM_018858680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD25"/>
    <dbReference type="STRING" id="869754.A0A1A0HD25"/>
    <dbReference type="GeneID" id="30031656"/>
    <dbReference type="OrthoDB" id="534912at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019740">
        <property type="term" value="P:nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900430">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3430.10:FF:000003">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43029:SF10">
        <property type="entry name" value="AMMONIUM TRANSPORTER MEP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="30"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="461"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="487"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="497" mass="53689" checksum="6D7BC134161A808B" modified="2016-10-05" version="1">MSTETFSGTGTGGDSLTQNLNLQFEGADMVWVGISSVLVWLMIPGVGLLYSGISRKKHALSLLWASVMAACVTTFQWFFWGYSLVFSHSNKSQFLGDLTNFCLMDVLGAPSVASSVPDILFCFFQGMFACVTAILMVGAGCERARLFPMMVFLFIWLTVVYCPIAYWVWGGNGWLINLGGLDFAGGGPVHENSGFAALAYSLVLGKRHDPLTNGKMPKYKPHSVTSIVFGTVFLWFGWFGFNGGSTGNATIRSWYACVNTNIAAASGGLTWMFVDYFRTGGKWSTVGLCMGAIAGLVGITPAAGYVPVYTAIIFGVVPAVACNFAVDLKNILRIDDGMDVFALHGVGGFLGSVMTGLFAADYVAATDGSTVIDGGWMNQHYIQLGYQLAGSVSIGAWSFVVTSIILLAMDRVPFLRLRLHEDEELMGVDLAEIGEYAYYFEEEGPDMMGPQSSPYVLEPIRSTDPKEKEAKTQEPVDGIASNGETFSPDESEEHSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD80</accession>
    <name>A0A1A0HD80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-rRNA-processing protein ESF2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">18S rRNA factor 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pre-rRNA-processing protein esf2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77436</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF2/ABP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712384.1">
        <property type="nucleotide sequence ID" value="XM_018858701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD80"/>
    <dbReference type="STRING" id="869754.A0A1A0HD80"/>
    <dbReference type="GeneID" id="30031677"/>
    <dbReference type="OrthoDB" id="287393at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12263">
        <property type="entry name" value="RRM_ABT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039119">
        <property type="entry name" value="ABT1/Esf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034353">
        <property type="entry name" value="ABT1/ESF2_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12311:SF7">
        <property type="entry name" value="ACTIVATOR OF BASAL TRANSCRIPTION 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="32"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="318" mass="36590" checksum="F567A6E4E383FB31" modified="2016-10-05" version="1">MTSYAIPSAHTTDTKITFIPVFTSERLVSLDDDFSSDDEEQSIFQTTKKKADHRFEAQQDSESDALDAEDEPESVSKTESGPKPKLDETSAMVEEQHSRANKLKRLTPEQLAKEQKKIKRTGVCYLSSIPPYMKPVKLRSVLSKFGKLDRIFLKPEDPTSYHRRVKYGGNKKKRFTEGWVEFTKKTDAKLCAETMNGNILGGKKSSYYHDDIINIKYLPGFKWFDLTQQISKENEIRQAKLALEISQQAKLDKSFVNNVERSKHYERKRKAQGDDVEEPKFKRDFSQHAIASTRADAKQKFKKEKNSDNLESVLSKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDB1</accession>
    <name>A0A1A0HDB1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HRQ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_77460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712414.1">
        <property type="nucleotide sequence ID" value="XM_018858705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDB1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDB1"/>
    <dbReference type="GeneID" id="30031681"/>
    <dbReference type="OrthoDB" id="497541at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="46073" checksum="D73B2A2D1DCB1A9A" modified="2016-10-05" version="1">MFVPLLDFLQLKHYVYLLVAVCLATAYYNKSKKTAKPTRVSSANKDRKFGTWVPDDFVTPVPLPFKNWDVNKTQPLPYRAFKHKYNVTMGIRNMEWDSWIELDNEWMKYHSEKIKRLDEKGTEVYGTLPGAEAAVLELLDEFWAYLPARYPSLFRQLDNGLENLLTGERFVFKNCTEYSEDPMVMAAKMVQDDLAIMIEGSDGNYVLKAGAIILPGFWRFKDKVGLPLNQIHTNGDVPKYQEKLQSGMTKFFTRLTCEKPVVRNNYFIQTDNALGWSTSIGDENSDVVGWYTAKPAETPDQIWYRSERQSLRRLPKTGAVVFTIRTYFCPMTEMCTEPYIPRRLLNGIESWTDDVREYRGYEKFKDVLLPYLEEKAQEQEKRGYVVENEPLVYPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDT8</accession>
    <name>A0A1A0HDT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase delta subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712667.1">
        <property type="nucleotide sequence ID" value="XM_018857138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDT8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDT8"/>
    <dbReference type="GeneID" id="30030114"/>
    <dbReference type="OrthoDB" id="514823at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.20">
        <property type="entry name" value="DNA polymerase delta, p66 (Cdc27) subunit, wHTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041913">
        <property type="entry name" value="POLD3_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="337"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="397" mass="44074" checksum="4A7D2098F6C83CE1" modified="2016-10-05" version="1">MDIAKEHVEYISSALKSHVVTYHTAARDLGIHVSKSKAMLLNYYQANKTRVAASFVATGTRLGNLAVRVFESEADLTENCDSFFDSVNTVHVYSLLANTNSFTLVAIAMEERIHCTNLEALDTYWDLGLIQGPTLERCLKARAAPSTIPKSHATAQLRPTESEETKSQSTPTDETEKPKPKLVYQSRKQQPKSSLLSSYVSRKSEKNSTTGKALGAPKRALLDRPLYEYKSRKLEQLQPKERVVVSTEGDENEVADELMEDAPPLTKPTVTESELHKMFLDDFTDDEKVDGALEMEVDQPIVVENTENEQSDAPMEEPAEPEIPQALLLPKNDIWKSMSSASPVPDSSTAKPASPAPETTVDDDGYFTLYKQSEPKPAPKTDGKKKQASVMSFFLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW9</accession>
    <name>A0A1A0HDW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_11046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712671.1">
        <property type="nucleotide sequence ID" value="XM_018853907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDW9"/>
    <dbReference type="GeneID" id="30026883"/>
    <dbReference type="OrthoDB" id="408954at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102772">
        <property type="term" value="F:sphingolipid C4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050307">
        <property type="entry name" value="Sterol_Desaturase_Related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11863">
        <property type="entry name" value="STEROL DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="5">
        <location>
            <begin position="150"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="342" mass="40204" checksum="43AD0FC53543BE73" modified="2016-10-05" version="1">MFAPPQNFSSMLASPHEPVSFAMKKPDLIPGISDGVLALLSPVIAYWTYATFFHIIDVYELAEKYRIHPSEEEQARNKASLKDVLYDVVIQHIIQTIVGYAVYRYDELPTTGHELHSMWNLRQTLFPTFVPDSFVWYGYMYGWSLLRLSIALCIIDTWQYWLHRTMHTNKVLYRRFHSRHHRLHVPYSYGALYNDPVEGFLLDTAGTGLAAIVLALSPRESLVLYTFATMKTVDDHCGYRFPFDPFQFLFPNNALYHDIHHQNWGFRSNYSQPFFTFWDKLTDTQYKFVSEYQEHQKQITLQKYKEFLEKRKQNKLSKDMAASDSTDSDILETQAKISKKMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ0</accession>
    <name>A0A1A0HDZ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Tricalbin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_31073</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712619.1">
        <property type="nucleotide sequence ID" value="XM_018855764.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HDZ0"/>
    <dbReference type="GeneID" id="30028740"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF1">
        <property type="entry name" value="TRICALBIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="13">
        <location>
            <begin position="192"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="390"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="540"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="677"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1009"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="12">
        <location>
            <begin position="1317"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1211"/>
            <end position="1316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="10"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="34"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1213"/>
            <end position="1237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1245"/>
            <end position="1260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1261"/>
            <end position="1271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1450" mass="160790" checksum="B0F18F3EF621D6C9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE09</accession>
    <name>A0A1A0HE09_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_148615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712711.1">
        <property type="nucleotide sequence ID" value="XM_018854551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE09"/>
    <dbReference type="STRING" id="869754.A0A1A0HE09"/>
    <dbReference type="GeneID" id="30027527"/>
    <dbReference type="OrthoDB" id="4091119at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291914" evidence="1">
        <location>
            <begin position="18"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="266" mass="27919" checksum="160BEDBD30B2EF32" modified="2016-10-05" version="1">MILPFAALTVLASVVSAIPFKRYDNETISATTAFSLPDTTITVDKYITTTLSGSSVSARSLSDLAANTQNIISVVTEYVTISTKGSAVVTPVTTFTTTMPESTTTSTLTSYVTITESDGTVSTEAIESGLIETAEACVCVPSTVTVTATNSVNDVEDQYSAITLISVTAIPVYADFTFSGLTTTITSYVEVTLTETTYSPIVFYSTTTGTSSTPDISSSMPTVTSSSAVTLSLTYSSPSMAPVYSSFNNQTVVSSGKSRRAFLYHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE22</accession>
    <name>A0A1A0HE22_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Snare-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_39128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712845.1">
        <property type="nucleotide sequence ID" value="XM_018857095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE22"/>
    <dbReference type="STRING" id="869754.A0A1A0HE22"/>
    <dbReference type="GeneID" id="30030071"/>
    <dbReference type="OrthoDB" id="18320at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="173" mass="19205" checksum="8D25D0C1679A61E3" modified="2016-10-05" version="1">MSTAPKTPTAAKTTAAKSASVLFVSLVSRDDKPLYIQSFEGAENAHALPQHAGQFLKYNFLSHMALDVFASPMSLHLREQQEDHDGVLLLFIQDDVYVYGMETNNGLKVVVGASNEIGARPALRLLFLRIHRAYLKTVCNPFTDVSSAGDCEQMLHTPAFERRMHALVGDWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE43</accession>
    <name>A0A1A0HE43_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA pol II transcription cofactor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_31122</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pirin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712669.1">
        <property type="nucleotide sequence ID" value="XM_018855772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE43"/>
    <dbReference type="STRING" id="869754.A0A1A0HE43"/>
    <dbReference type="GeneID" id="30028748"/>
    <dbReference type="OrthoDB" id="198735at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02247">
        <property type="entry name" value="cupin_pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02909">
        <property type="entry name" value="cupin_pirin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903:SF8">
        <property type="entry name" value="PIRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PIRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin N-terminal" evidence="4">
        <location>
            <begin position="23"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin C-terminal" evidence="5">
        <location>
            <begin position="176"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006232-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="334" mass="37998" checksum="05F7255F701F3540" modified="2016-10-05" version="1">MSTLRTIKTILPGIEQAEGVGATVRRSIGNIKMRSFSPFLLFDHFSSNGVGGFPEHPHLGQETITLMTKGAFAHEDFTGSKGVLREGDLQFMTAGRGTVHSEMPVIHPDGSFNSGLQLWVDLPEKLKEVEPRYRDLKTWEIPTVEQQEGKVKVRVISGKSYGVELLKELAYTPVQYYHYMMKPGSHFSQEIPNSFNFFLYVMKGNNLILQKNTKVSQYENVFFNPDGDHITGDYSETEKEDLEFVLIGGQILDQKVVQHGPFVATSRERIQQAFLDYQMARNGFERLRTWKSLISSGVTDEMVDGELRGSLEAREEERKKYISKWKEGVIDENC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE58</accession>
    <name>A0A1A0HE58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-splicing endonuclease subunit Sen15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_39931</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEN15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712890.1">
        <property type="nucleotide sequence ID" value="XM_018857206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE58"/>
    <dbReference type="STRING" id="869754.A0A1A0HE58"/>
    <dbReference type="GeneID" id="30030182"/>
    <dbReference type="OrthoDB" id="10002170at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042777">
        <property type="entry name" value="Sen15_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018593">
        <property type="entry name" value="tRNA-endonuc_su_Sen15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28518">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28518:SF1">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09631">
        <property type="entry name" value="Sen15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA-splicing endonuclease subunit Sen15" evidence="3">
        <location>
            <begin position="6"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="109" mass="12475" checksum="DF8225368DE45EF4" modified="2016-10-05" version="1">MDLAAQVEKNLVHYNLWTDVRVFRKDDRFAVLQGQPPSKLAENDAMQPEWVVPKSMNDVKCSMAEIAAWFDYVATISERPKRVTMAIVQDDGTVVYYFVHDGVTAPRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE68</accession>
    <name>A0A1A0HE68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_82362</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712900.1">
        <property type="nucleotide sequence ID" value="XM_018859220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE68"/>
    <dbReference type="GeneID" id="30032195"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508827" evidence="1">
        <location>
            <begin position="32"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="110" mass="12121" checksum="128EA16CB3AE8F01" modified="2016-10-05" version="1">MSSSQSFTLGPALLAIPLSLLLCLIASSGSAMCRKRPPAPISSIYKPARYLINPQKYSARIGGQGNRHFPVPNPVTLRFRTFRLHFTSMLNIDTLLTGMLSTDMIQTEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE95</accession>
    <name>A0A1A0HE95_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712718.1">
        <property type="nucleotide sequence ID" value="XM_018855783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE95"/>
    <dbReference type="GeneID" id="30028759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="59" mass="6694" checksum="381F66E12924D5C5" modified="2016-10-05" version="1">MVNLEGVRRICIRVFAPIYLSEAEDNYIPLLFPFERKLFAVVSKINVVALVLLVSIITS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEA5</accession>
    <name>A0A1A0HEA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="3 12">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_31166</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712728.1">
        <property type="nucleotide sequence ID" value="XM_018855785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEA5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEA5"/>
    <dbReference type="GeneID" id="30028761"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000040">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="344"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="392" mass="43553" checksum="EEA4B5C725B02EB8" modified="2016-10-05" version="1">MNVDEKFDLITKGLQEVLNPQILKDVLLKNERPVRIYWGTAPTGKPHCGYFVPMIKLAHFLKAGCEVKVLIADLHAYLDSMKAPLEVVQYRAKYYEHVVKTILKSINVPVEKLKFIVGSEYQLTPEYTMDLFKLSSKVSQNDAKRAGADVVKQNDNPLLSGLIYPLMQALDEQYLDVDVQFGGVDQRKIFVLAEENLPGIGYKKRAHLMNPMVPGLGQGAKMSASDPNSKIGVTDAPNVVKKKIASAFCAPGNIEENGLLSFLENIVQPIQELKSGKEGIFNFFIDRPEKYGGPIEYSSFKHLKSDFAAEKLSPVDLKSGVTDKINELLAPIIEAFENDKDFQDADKQGYPVEAPKEKKSKKVKNKGTFYPGNKAAPSNVEDATKKLEATSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEA8</accession>
    <name>A0A1A0HEA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_31171</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712734.1">
        <property type="nucleotide sequence ID" value="XM_018855788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEA8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEA8"/>
    <dbReference type="GeneID" id="30028764"/>
    <dbReference type="OrthoDB" id="10264505at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291766" evidence="3">
        <location>
            <begin position="23"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="310" mass="36557" checksum="8CBD9F9557974FBE" modified="2016-10-05" version="1">MIFTWVLLQFLLSSCLLHVIEVSSIEEIVTKPVSLIYFYAPDCQYCQLFDDDFQYLSHIYSENSKFQIIKINGREQKYFKELFDVKLYPTLKLYDHLNKQVITFKDGRLIENLQDFIKKHAEVEPDLTRVKQELNAILLKADVERLQKIGRSVLIAFVRRQDEYWEKYHYPDHFYQSIGRNHPSIEFTVNFFEDGEFELMELYHVSNIPALVLIEGSHIGVYNTLSTNPMSNYMLDANDVEAFLSDFHSKKEGTWFQDVHLLARYAESSQYLGHKQRKTGMNYVEGVVKGKMSELGIEEQYRILLDKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEC5</accession>
    <name>A0A1A0HEC5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77852</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712960.1">
        <property type="nucleotide sequence ID" value="XM_018858781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEC5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEC5"/>
    <dbReference type="GeneID" id="30031757"/>
    <dbReference type="OrthoDB" id="20295at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000055">
        <property type="entry name" value="Ubiquitin conjugation factor e4 a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="12">
        <location>
            <begin position="910"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="965" mass="109864" checksum="343C1A9080B86A97" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF28</accession>
    <name>A0A1A0HF28_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RabGAP/TBC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77940</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713081.1">
        <property type="nucleotide sequence ID" value="XM_018858792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF28"/>
    <dbReference type="STRING" id="869754.A0A1A0HF28"/>
    <dbReference type="GeneID" id="30031768"/>
    <dbReference type="OrthoDB" id="295078at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23436">
        <property type="entry name" value="RabGap-TBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="150"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="573" mass="64719" checksum="FFDBECC8E8D8B479" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF37</accession>
    <name>A0A1A0HF37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Phosphoglucomutase/phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_39418</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713091.1">
        <property type="nucleotide sequence ID" value="XM_018857137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF37"/>
    <dbReference type="STRING" id="869754.A0A1A0HF37"/>
    <dbReference type="GeneID" id="30030113"/>
    <dbReference type="OrthoDB" id="8300170at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="49"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="155"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="249"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="359"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="631" mass="68925" checksum="9BD22D9844F61AD8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF57</accession>
    <name>A0A1A0HF57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_163552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713111.1">
        <property type="nucleotide sequence ID" value="XM_018854637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF57"/>
    <dbReference type="GeneID" id="30027613"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="146" mass="15537" checksum="92D135FD60D27610" modified="2016-10-05" version="1">MSDIMSMVSSMTELTDMSTMTDMMSSMTDMSFMTDMSSMTDMMDSSMTDMMDSSMMMDSSMMMDSSMMMDSSMMMDSSMMMDSSMMTSMSDMMSSTMSSMMSDSSASGNSSSSSTASSGAEAVAASWKKGAAIAALVGFSFAVNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF93</accession>
    <name>A0A1A0HF93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713058.1">
        <property type="nucleotide sequence ID" value="XM_018855851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF93"/>
    <dbReference type="GeneID" id="30028827"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="93" mass="10959" checksum="1A16990170C1E3CF" modified="2016-10-05" version="1">MYMDFRFEVHCVNFRKSQRFAAYWTHTLASSNLVIFFLLFSQLRHFTSTRPVQTTDCFASRIPIEFNFASSIGRLIYIWPAKAIYIHPASSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFF3</accession>
    <name>A0A1A0HFF3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Histone deacetylase</fullName>
            <ecNumber evidence="2 9">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_36343</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713196.1">
        <property type="nucleotide sequence ID" value="XM_018856724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFF3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFF3"/>
    <dbReference type="GeneID" id="30029700"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034967">
        <property type="term" value="C:Set3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045835">
        <property type="term" value="P:negative regulation of meiotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032874">
        <property type="term" value="P:positive regulation of stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000007">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF36">
        <property type="entry name" value="HISTONE DEACETYLASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="14">
        <location>
            <begin position="46"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="415"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="448" mass="50545" checksum="FF7A3A34990AFF2A" modified="2016-10-05" version="1">MSATFSTDTKTTAQQPLYQMLRQLALKNVLYHYNNQAAHFHYGPHHPMKPFRLMLTDHLVLLYKLHEKMDLYEPRAATKDELMAFHSEDYVEFLQRITPELAAKMDTDKLAQFNIGDDCPIFDGMYAYSLVYSGATLDASRKLISGMSDIAINWSGGLHHAKKAEPSGFCYANDIVLAILNLLRVHARVMYIDIDLHHGDGVQEAFNLTDRVMTVSFHKFNGEFFPGTGAIDEVGIDKGLGYSVNVPLKDGIDDDSYLALYKLIVEPLITKFRPTCIVQQCGADSLGYDRLGCFNLNIRAHGECVKFTKSFGIPMLVLGGGGYTPRNVSRLWCYETSVLNDVTLEPKIPSSIPTYEWFGPDYSLHPPLDGRLDNKNLKKYLDCVRQHVLEQLRTIDAAPLVRMDEIPPDLSGLTEEEEEMLKELNEDTAQGRESVRAKEEARTGEVHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM0</accession>
    <name>A0A1A0HFM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PC4-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_90348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transcriptional coactivator PC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713173.1">
        <property type="nucleotide sequence ID" value="XM_018859301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFM0"/>
    <dbReference type="GeneID" id="30032276"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.31.10">
        <property type="entry name" value="Transcriptional Coactivator Pc4, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003173">
        <property type="entry name" value="PC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009044">
        <property type="entry name" value="ssDNA-bd_transcriptional_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045125">
        <property type="entry name" value="Sub1/Tcp4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13215">
        <property type="entry name" value="RNA POLYMERASE II TRANSCRIPTIONAL COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02229">
        <property type="entry name" value="PC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54447">
        <property type="entry name" value="ssDNA-binding transcriptional regulator domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcriptional coactivator p15 (PC4) C-terminal" evidence="8">
        <location>
            <begin position="22"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="83"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="120" mass="13282" checksum="B1EA0BA4B9E7E67D" modified="2016-10-05" version="1">MAYPTKAAKRTAPSEENVESVFELDAKKQVTVRKFNGINLVDIREFYIDKNTQEKKPGKKGISLTAALWRKLLDQKDEIDKALGALDGEPKKAKKDESSMPADGGNEAKAEESEAQGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFV6</accession>
    <name>A0A1A0HFV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hyaluronan/mRNA-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_48413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713219.1">
        <property type="nucleotide sequence ID" value="XM_018858280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFV6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFV6"/>
    <dbReference type="GeneID" id="30031256"/>
    <dbReference type="OrthoDB" id="2122308at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="8438" checksum="A573411A32F01CFF" modified="2016-10-05" version="1" fragment="single">MTRTKKWTVHEKASTPRFFTHNGHFNTDPNKVNKNGSGKFNWGKPGDELMDESEFPSTGRRNSNHMANEQDMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG11</accession>
    <name>A0A1A0HG11_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713274.1">
        <property type="nucleotide sequence ID" value="XM_018855390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG11"/>
    <dbReference type="GeneID" id="30028366"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="8623" checksum="9C887450AB12A621" modified="2016-10-05" version="1">MRVLIVGFGMRVLVCGFWIVGVWIVGVWIVGVWIVGVWIVGVWIVGVWIVGVIIVGVIIVGVIIVGILGFLAFRIPRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG48</accession>
    <name>A0A1A0HG48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37124</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713441.1">
        <property type="nucleotide sequence ID" value="XM_018856822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG48"/>
    <dbReference type="STRING" id="869754.A0A1A0HG48"/>
    <dbReference type="GeneID" id="30029798"/>
    <dbReference type="OrthoDB" id="10265467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18139">
        <property type="entry name" value="HLD_clamp_RarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3710.10:FF:000005">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000001">
        <property type="entry name" value="Putative AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000137">
        <property type="entry name" value="Replication-associated recombination protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3710.10">
        <property type="entry name" value="DNA polymerase III clamp loader subunits, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051314">
        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="198"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="95"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="706" mass="78790" checksum="11459B16F0367F12" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG50</accession>
    <name>A0A1A0HG50_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_18567</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713314.1">
        <property type="nucleotide sequence ID" value="XM_018854793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG50"/>
    <dbReference type="STRING" id="869754.A0A1A0HG50"/>
    <dbReference type="GeneID" id="30027769"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14131">
        <property type="entry name" value="PKc_Mps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000377">
        <property type="entry name" value="Checkpoint protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027084">
        <property type="entry name" value="Mps1_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974:SF21">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="37461" checksum="BF780A787A5EECDA" modified="2016-10-05" version="1" fragment="single">LNSKQYEKLEVLGKGGTSKVYKVRSLSDRKAYALKKVSFDQFDESCVRGFKGEIDLLTKLKHEPRVVSLIDHASSAGSLYLLMECGDLDLAHALQKRMYLDTKLDPNFVRFHASEVFQCIQAVHNAGVVHSDLKPANFLFVDGILKIIDFGIANAVPDHTVNIYRNSQIGTPNYMAPEALVEVNSTLSLLAKNAATTWRVGRPSDIWSCGCIIYQMIYGRPPYASYSGQQRIMAIMNPQVKVLYPATGLGGVRVPASAITLMKKCLERNPSDRWTVEECLHCDFLKPKAVDEHFVKDLLHNAVTFVQRNTHPGSIDEKTYTELVDTILGQIKDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGC7</accession>
    <name>A0A1A0HGC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Signal peptidase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_36169</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Essential for the SPC catalytic activity, possibly by stabilizing and positioning the active center of the complex close to the lumenal surface. Essential for viability.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SPCS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713526.1">
        <property type="nucleotide sequence ID" value="XM_018856700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGC7"/>
    <dbReference type="STRING" id="869754.A0A1A0HGC7"/>
    <dbReference type="GeneID" id="30029676"/>
    <dbReference type="OrthoDB" id="10261524at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 23 KD SUBUNIT SPC22/23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12804:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016089">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR016089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="189" mass="20927" checksum="E7A25768735A21FD" modified="2016-10-05" version="1">MFSLASRVQALSNTTLTAVSLISAIVAIISVAQLYFAGAWSLDGTNISHVKALALLKNLRSFGAEPGSAKENAKIKFDLDADLSPLFNWNTKQVFVYLTAEYDGANSGENKVTFWDKIITNKEDANLSLKSVSSKYSVWDVEPSFRERSARLKLEWNVQPWVGPLLYGQTEAEQSFQFAAAKKPKKTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGE2</accession>
    <name>A0A1A0HGE2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PRTase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_29600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713541.1">
        <property type="nucleotide sequence ID" value="XM_018855446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGE2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGE2"/>
    <dbReference type="GeneID" id="30028422"/>
    <dbReference type="OrthoDB" id="9973266at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000310">
        <property type="term" value="F:xanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046100">
        <property type="term" value="P:hypoxanthine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032265">
        <property type="term" value="P:XMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000033">
        <property type="entry name" value="Xanthine phosphoribosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="14"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="205" mass="23120" checksum="98785DB976893CA5" modified="2016-10-05" version="1">MPEKLYISYNIVHKLCQKAAQEIKASGMKVDLIIAIGGGGFIPARILRSFLKDGSKNIPIQAVGLSLYEQMGNTTEEMIGKEVVRTQWIDFGNLSQHLDTLIGKNIIIVDEVDDTRTTLHYAVSELQRDVARVEKELARDGETTNMSVFVLHNKKKPKRAELPQAMLDSGRYFVGENVDDVWIAYPWDAQDIDEHTEAAKRQGNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGF2</accession>
    <name>A0A1A0HGF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anaphase-promoting complex subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_36960</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713551.1">
        <property type="nucleotide sequence ID" value="XM_018856808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGF2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGF2"/>
    <dbReference type="GeneID" id="30029784"/>
    <dbReference type="OrthoDB" id="1681166at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16456">
        <property type="entry name" value="RING-H2_APC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051031">
        <property type="entry name" value="RING-box_E3_Ubiquitin_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024991">
        <property type="entry name" value="RING-H2_APC11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11210">
        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12861">
        <property type="entry name" value="zf-ANAPC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="38"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="126" mass="14151" checksum="D4B9556FDC69AE26" modified="2016-10-05" version="1">MKVKIKHWHGVATWHWHTQSSQNDELCGICRVPFDGTCPNCKYPGDTCPLILGATCSHNFHLHCILKWLEQSNSKGLCPMCRQIFTAKVIEGIGSPQELAELQALIESHQNGRDAELGTDFEYGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGX5</accession>
    <name>A0A1A0HGX5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">methylmalonate-semialdehyde dehydrogenase (CoA acylating)</fullName>
            <ecNumber evidence="2">1.2.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_105130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.27" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713910.1">
        <property type="nucleotide sequence ID" value="XM_018853787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGX5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGX5"/>
    <dbReference type="GeneID" id="30026763"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004491">
        <property type="term" value="F:methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006210">
        <property type="term" value="P:thymine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07085">
        <property type="entry name" value="ALDH_F6_MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000002">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase (Acylating)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000003">
        <property type="entry name" value="Methylmalonate-semialdehyde dehydrogenase [acylating]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010061">
        <property type="entry name" value="MeMal-semiAld_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01722">
        <property type="entry name" value="MMSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866">
        <property type="entry name" value="MALONATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43866:SF3">
        <property type="entry name" value="METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="5">
        <location>
            <begin position="55"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="543" mass="58391" checksum="87DF2611EB40231A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH18</accession>
    <name>A0A1A0HH18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_37960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BLOC1S2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713771.1">
        <property type="nucleotide sequence ID" value="XM_018856933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH18"/>
    <dbReference type="GeneID" id="30029909"/>
    <dbReference type="OrthoDB" id="244061at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019269">
        <property type="entry name" value="BLOC1_su2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10046">
        <property type="entry name" value="BLOC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="51"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="110" mass="12342" checksum="CE43F76318E74FC0" modified="2016-10-05" version="1">MALAPKDTGRLVAHALTLAQKVVDADTEVSTIDLQLLSNLNTNQFLNYINLDQQLSTLEQDTEKLAVYKQEVVLLTLDLDQIERQVDALMNVTNELLSWCNELAATKQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHG2</accession>
    <name>A0A1A0HHG2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_29822</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713798.1">
        <property type="nucleotide sequence ID" value="XM_018855487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHG2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHG2"/>
    <dbReference type="GeneID" id="30028463"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="13">
        <location>
            <begin position="70"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="14">
        <location>
            <begin position="432"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="562" mass="63645" checksum="E179513811326621" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHN4</accession>
    <name>A0A1A0HHN4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_64326</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713997.1">
        <property type="nucleotide sequence ID" value="XM_018858343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHN4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHN4"/>
    <dbReference type="GeneID" id="30031319"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048203">
        <property type="term" value="P:vesicle targeting, trans-Golgi to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016342">
        <property type="entry name" value="AP_complex_bsu_1_2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002291">
        <property type="entry name" value="AP_complex_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="8">
        <location>
            <begin position="17"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="715"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="735" mass="78651" checksum="67D1BE07C88E62F1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHQ1</accession>
    <name>A0A1A0HHQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76535</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714016.1">
        <property type="nucleotide sequence ID" value="XM_018858546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHQ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHQ1"/>
    <dbReference type="GeneID" id="30031522"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051063">
        <property type="entry name" value="PDI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE C17H9.14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45672:SF3">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291848" description="Thioredoxin domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="675"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="33"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="715" mass="81930" checksum="4F2575A6B52C3EA3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHW7</accession>
    <name>A0A1A0HHW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_64509</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714077.1">
        <property type="nucleotide sequence ID" value="XM_018858346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHW7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHW7"/>
    <dbReference type="GeneID" id="30031322"/>
    <dbReference type="OrthoDB" id="10264412at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07960">
        <property type="entry name" value="Anticodon_Ia_Ile_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="entry name" value="Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02002">
        <property type="entry name" value="Ile_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033708">
        <property type="entry name" value="Anticodon_Ile_BEm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023585">
        <property type="entry name" value="Ile-tRNA-ligase_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050081">
        <property type="entry name" value="Ile-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42765">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="10">
        <location>
            <begin position="53"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="11">
        <location>
            <begin position="718"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="969" mass="110177" checksum="336210A81A9030E8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIA6</accession>
    <name>A0A1A0HIA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_38032</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714098.1">
        <property type="nucleotide sequence ID" value="XM_018856947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIA6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIA6"/>
    <dbReference type="GeneID" id="30029923"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902413">
        <property type="term" value="P:negative regulation of mitotic cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905665">
        <property type="term" value="P:positive regulation of calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050850">
        <property type="term" value="P:positive regulation of calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032995">
        <property type="term" value="P:regulation of fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07857">
        <property type="entry name" value="STKc_MPK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000013">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008352">
        <property type="entry name" value="MAPK_HOG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01773">
        <property type="entry name" value="P38MAPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="22"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="384"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="485" mass="55684" checksum="CA57DA455931684A" modified="2016-10-05" version="1">MAQNGRLVNKVFNQDFVIDKRFKITKELGHGAYGIVCSAQYDDGSGSPGGNNVAIKKITNIFSKKILCKRALREVKLLQFFRGHKNITCLYDLDIVMDPVTGVFNDIYLYEELMECDMHQIIRLGQPLTDLHYQLFIYQILCGLKYIHSADVLHRDLKPGNLLVNVECDLKICDFGLARGFSEDVEQNSGFMTEYVATRWYRAPEIMLSFSNYTKAIDIWSVGCILGELLGGKPLFRGKDYVDQLNQILMVLGTPKESALTKIGSPRAQNYVRSLPIMRKIHYREILPNANPLALDLLERMLAFDPHERITVEEALNHPYLAVWHDPQDEPESKIKFDFKTFETVDDMESMKQLIIEEVKSFREFVRKPIQEQKQIQLQIQEKQQQQQQREADQEQLRKNSKSIPGGEFSDQFGGIMTSNASVLNNTGLLTDPSQLRNEDNLQHYDAMPKPQEFEEFSLLARNEDPNLLLEEELGFGMDGSAFHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIE6</accession>
    <name>A0A1A0HIE6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714261.1">
        <property type="nucleotide sequence ID" value="XM_018853669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIE6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIE6"/>
    <dbReference type="GeneID" id="30026645"/>
    <dbReference type="OrthoDB" id="4035953at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014805">
        <property type="entry name" value="SKG6/TOS2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08693">
        <property type="entry name" value="SKG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="603"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="611"/>
            <end position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="689" mass="72350" checksum="4914F37ABB9E9CCA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH1</accession>
    <name>A0A1A0HIH1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_9922</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714158.1">
        <property type="nucleotide sequence ID" value="XM_018859490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH1"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH1"/>
    <dbReference type="GeneID" id="30032465"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="43"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="35364" checksum="78D6292F073C30B9" modified="2016-10-05" version="1">MSTFSKSGFKSLNYNSFRPQYPPPFYQLLLDYTGKTRIPHAIDLGCGTGVASFPLLNFCDKVVGLDLSPLMISGANKLKQNRLQEMGITDESRINFEVAAVEEYKAPPESSDLIVAAECIHWFKDYDTFFTAAAKQLRPGATLAYWYYVDPLIVDFNGPSSLNLSKQDILINVMDAYDLMVYRDESLLGPHWEQPGRSILQGFLTEVDKHIPRELFTDIKIRKYMPGSLNTYRNDDLQLVRKDISLLDYTKYISTYSSYHNYDEATGKGEELLDNFLRKLENEFGWDRNNTTLTLEWYAGYTFMKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIZ4</accession>
    <name>A0A1A0HIZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein HIR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_65768</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Required for replication-independent chromatin assembly and for the periodic repression of histone gene transcription during the cell cycle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the WD repeat HIR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714610.1">
        <property type="nucleotide sequence ID" value="XM_018858356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIZ4"/>
    <dbReference type="GeneID" id="30031332"/>
    <dbReference type="OrthoDB" id="1741719at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905268">
        <property type="term" value="P:negative regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016480">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001073">
        <property type="entry name" value="Protein HIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055410">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011494">
        <property type="entry name" value="HIRA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019015">
        <property type="entry name" value="HIRA_B_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF0">
        <property type="entry name" value="PROTEIN HIRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24105">
        <property type="entry name" value="Beta-prop_CAF1B_HIR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07569">
        <property type="entry name" value="Hira"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09453">
        <property type="entry name" value="HIRA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 11 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="22"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="CAF1B/HIR1 beta-propeller" evidence="15">
        <location>
            <begin position="28"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="72"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="132"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HIRA-like C-terminal" evidence="14">
        <location>
            <begin position="750"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="644"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="644"/>
            <end position="654"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF07569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF24105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA24129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="949" mass="103832" checksum="E60510BEF2829E7A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ25</accession>
    <name>A0A1A0HJ25_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34590</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714368.1">
        <property type="nucleotide sequence ID" value="XM_018856494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ25"/>
    <dbReference type="GeneID" id="30029470"/>
    <dbReference type="OrthoDB" id="69177at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004656">
        <property type="term" value="F:procollagen-proline 4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045054">
        <property type="entry name" value="P4HA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10869:SF236">
        <property type="entry name" value="PROLYL 4-HYDROXYLASE ALPHA SUBUNIT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="131"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="241" mass="27818" checksum="485CCFC385BD0847" modified="2016-10-05" version="1">MAQRKQRSVSEELRVYDFPQNLYTLNANFKSSFFKPVPESVVENQIITIEHFFTDEFCNELITDFSNKLTLETTPLIKSREYAARHNDRVSVTSLPAADALWKYLRSVLMEVPQYDASELDLIRQIFKNAKSLNPQLRIYRYIKGHHFGKHYDESVSCPLAHDAKIKGKTKWTLLIYLTGDDEFSGGGTIFHPEIRDSMPLNIHPSKGMALLHKHGDDCLKHEAEIVRSGVKWVLRSDVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ36</accession>
    <name>A0A1A0HJ36_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_77020</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714650.1">
        <property type="nucleotide sequence ID" value="XM_018858623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ36"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ36"/>
    <dbReference type="GeneID" id="30031599"/>
    <dbReference type="OrthoDB" id="27911at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000400">
        <property type="entry name" value="Elongator acetyltransferase complex subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="54"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="193"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="620"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="668"/>
            <end position="702"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="811" mass="85950" checksum="91B7F36BD4091732" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ58</accession>
    <name>A0A1A0HJ58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_30523</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714670.1">
        <property type="nucleotide sequence ID" value="XM_018855653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ58"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ58"/>
    <dbReference type="GeneID" id="30028629"/>
    <dbReference type="OrthoDB" id="2020720at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01717">
        <property type="entry name" value="Sm_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="13569" checksum="D20920BFDDA6616E" modified="2016-10-05" version="1">MADLINYRLKVSILDGRTFSGQLLAFDKHMNVVLADTEEARVTKKAYRELAKTKVDGQVKIPEEKRFLGLVILRGEQVVNVTIISGPTADIKKRLTLLKQGKGVAKPLKIAGTSSKLRGPVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA6</accession>
    <name>A0A1A0HJA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714562.1">
        <property type="nucleotide sequence ID" value="XM_018853724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA6"/>
    <dbReference type="GeneID" id="30026700"/>
    <dbReference type="OrthoDB" id="4084610at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="1">
        <location>
            <begin position="293"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="821" mass="94113" checksum="3A5E881D2416CF0A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF2</accession>
    <name>A0A1A0HJF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF3074 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10169</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714449.1">
        <property type="nucleotide sequence ID" value="XM_018853699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJF2"/>
    <dbReference type="GeneID" id="30026675"/>
    <dbReference type="OrthoDB" id="6423603at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024500">
        <property type="entry name" value="DUF3074"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370:SF1">
        <property type="entry name" value="DUF3074 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40370">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11274">
        <property type="entry name" value="DUF3074"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3074" evidence="1">
        <location>
            <begin position="94"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="28261" checksum="94E8C8E282B1721D" modified="2016-10-05" version="1">MNSTPLKSLDTVTSSLLLENAAHIADLIQSWKAAGIYKYDVPQTQEKIEVGVRTKDHLNNDFWVARLNTFEELSPDGRKRLWEQLFKYSIGSTKDLQDCHTVHEQHYIEELYDFDISAYTLEDTPEGYESFSYLVETFYKLQWPLKRRRFSNLVHILKSTDERTAFVISIAVDPSLIPNATKDATLIDAQYTSVERLEYDGTNLLWLMTTCSDAKGNVPQWLARSSINSVVAKDVPNFLKWVGKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJM8</accession>
    <name>A0A1A0HJM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714682.1">
        <property type="nucleotide sequence ID" value="XM_018853749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJM8"/>
    <dbReference type="GeneID" id="30026725"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="241" mass="24317" checksum="C25516B92DFAD249" modified="2016-10-05" version="1">MGIKDKLKRSSRVFSSDCKESVSENGSSTAGAKEEVKTTTEPKAVPDAAEAAEAACTAKAATPEADAAIDAALDDVTGNEHVDENAAVSKGEGARDAAADAANPAPAAEAAAEVAAEDGKKPVKEGNAIKRSLSKRAKSKANTSTASGEARRGEIFHKFMKKFRRPVGVPAGKSAQKSADKTAKKVEVSGADKAETSTDNAAVATEAGAVDAVNAARDALLDPATAPEAVAETAGTVEPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJW2</accession>
    <name>A0A1A0HJW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">AP-3 complex subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_77132</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Adaptor protein complex 3 (AP-3) is a heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714791.1">
        <property type="nucleotide sequence ID" value="XM_018858648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJW2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJW2"/>
    <dbReference type="GeneID" id="30031624"/>
    <dbReference type="OrthoDB" id="10264595at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017105">
        <property type="entry name" value="AP3_complex_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781:SF12">
        <property type="entry name" value="AP-3 COMPLEX SUBUNIT DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22781">
        <property type="entry name" value="DELTA ADAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037092">
        <property type="entry name" value="AP3_complex_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="9">
        <location>
            <begin position="51"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="693"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="823"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="851"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="983"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="702"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="724"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="823"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="851"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="901"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="992"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1004"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1031"/>
            <end position="1043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1043" mass="117889" checksum="068D82F8DB4FB069" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKQ4</accession>
    <name>A0A1A0HKQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_30736</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the cullin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714953.1">
        <property type="nucleotide sequence ID" value="XM_018855693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HKQ4"/>
    <dbReference type="GeneID" id="30028669"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="5">
        <location>
            <begin position="415"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="760" mass="87007" checksum="1E29B34984BFD7CA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKT1</accession>
    <name>A0A1A0HKT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_134544</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714983.1">
        <property type="nucleotide sequence ID" value="XM_018854366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKT1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKT1"/>
    <dbReference type="GeneID" id="30027342"/>
    <dbReference type="OrthoDB" id="2687452at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051061">
        <property type="entry name" value="Zinc_finger_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179:SF13">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46179">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="181"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="371" mass="40875" checksum="3C431E4B04AA7B97" modified="2016-10-05" version="1">MSSTPNSSISNDGKSPTYALDQAHTSLQPPYGLRLGTILTLPPPQKAPLVTGTAASQIYPHPLNYPSQNLGAQLLPNIKAPPLKFGYPAVDSFNYPSLNQPQGPATPKLPQLPQVSLQNLPQTSFPLSTPKFPAVNDKALLQVFQPPQGIFQAFAPQNHLLPPQFPTQSLEVKSLVANGNTTLNTSSEPAYSKQIRYSSSDSENEKSPQQDGYNHTPKFIHSKHSKASKDAKLNRLKFTSALTPEYKPFVCASEGCNWSFARKSDLRRHAKSHIAPGFHCPYWKNDPTCHRNGGSFSRLDVLKRHLRLVHYIKDKDKAHSAADPGWCRACQKLFLTSKSFISHCEDCASQVAPAEWKANTSPKEQTSVADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKV5</accession>
    <name>A0A1A0HKV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPP transmembrane region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_35885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714919.1">
        <property type="nucleotide sequence ID" value="XM_018856665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HKV5"/>
    <dbReference type="GeneID" id="30029641"/>
    <dbReference type="OrthoDB" id="2016548at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007065">
        <property type="entry name" value="HPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058581">
        <property type="entry name" value="TM_HPP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33741:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN DDB_G0269096-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="TM_HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="HPP transmembrane region" evidence="2">
        <location>
            <begin position="42"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="282" mass="30911" checksum="0E451206B80A379B" modified="2016-10-05" version="1">MFKFTIDDAINRYLWPSQVSRLPSPVARFLGGSTPRPVSDLWLWLEILVSTFCGIAVIEAIFKTHTVFSDHNAPLIIASYGASAILCFNANGSPLAQPRNVIMGQFLSSLIGLCVQKLFLLSSAGRDNYWAGGALSVAILSVAMSIFNCVHPPGGASALLPCTSEEIQRMSWWYLPAQLVSSVVMVAVACIFGNILRSYPSYWWSGGIMGKPLEDTEKDKEDVLLLPSTVRSEKSHALRYVDGLPRIEIDGGFINLPAGLELSNLEKDWLTSLQKLMAKLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYW9</accession>
    <name>A0A1A0GYW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_80211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709268.1">
        <property type="nucleotide sequence ID" value="XM_018859195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYW9"/>
    <dbReference type="STRING" id="869754.A0A1A0GYW9"/>
    <dbReference type="GeneID" id="30032171"/>
    <dbReference type="OrthoDB" id="4087429at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="383" mass="44147" checksum="62D8253DC730FFB5" modified="2016-10-05" version="1">MILDSMLCLSSLISQRKDPLSVDALCPYDYYQKCVYSVGKNVALDKAQDPDGKILARCLISTNLLCIYEMFFVAVDSVYVKGAGGILATIMARTDKSGGILQQSPFHYTCFWATVVCDLILSLKLECPCTFSIEKMWRSLDPGYDEYDSYEAFLHEKTSIKDAVEDCSSFVVTNEKTTWWLHKVTFLFILVNDFYNQYDVITQEDFMTNKRFHRWLVLKEKIEEFERRMPIYLKPLIFIPAAEEKDFPKIFFKDEKTVVTAINFRLAKLLLHAALEEKLQVLDVGLTLKERASYPHKYSENLAREVAGIMKTYDADMRIWPINIHSLRQASRFIEPSSPCFETLKNLTARVISVCQTRLNILDLMEDKAENSRPASLHSFVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ74</accession>
    <name>A0A1A0GZ74_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_136602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709325.1">
        <property type="nucleotide sequence ID" value="XM_018854404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ74"/>
    <dbReference type="GeneID" id="30027380"/>
    <dbReference type="OrthoDB" id="4089867at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="597"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="772"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="946"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="995" mass="110427" checksum="46C488BE4429A0C9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1W1</accession>
    <name>A0A1A0H1W1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Checkpoint protein RAD24-like helical bundle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_47732</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad17/RAD24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709411.1">
        <property type="nucleotide sequence ID" value="XM_018858211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1W1"/>
    <dbReference type="STRING" id="869754.A0A1A0H1W1"/>
    <dbReference type="GeneID" id="30031187"/>
    <dbReference type="OrthoDB" id="10265971at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004582">
        <property type="entry name" value="Checkpoint_prot_Rad17_Rad24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057927">
        <property type="entry name" value="RAD24-like_helical"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12172:SF0">
        <property type="entry name" value="CELL CYCLE CHECKPOINT PROTEIN RAD17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03215">
        <property type="entry name" value="Rad17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25812">
        <property type="entry name" value="RAD24_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Checkpoint protein RAD24-like helical bundle" evidence="9">
        <location>
            <begin position="304"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="527"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="567"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="536"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="567"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA18016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="604" mass="68000" checksum="43405845B3D558C5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H205</accession>
    <name>A0A1A0H205_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">20S-pre-rRNA D-site endonuclease NOB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33745</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the synthesis of 40S ribosome subunits. Has a role in processing 20S pre-rRNA into the mature 18S rRNA, where it is required for cleavage at the 3' end of the mature 18S rRNA (D-site). Accompanies the 20S pre-rRNA from the nucleus to the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
            <location evidence="7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NOB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709380.1">
        <property type="nucleotide sequence ID" value="XM_018856391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H205"/>
    <dbReference type="STRING" id="869754.A0A1A0H205"/>
    <dbReference type="GeneID" id="30029367"/>
    <dbReference type="OrthoDB" id="446759at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017117">
        <property type="entry name" value="Nob1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037125">
        <property type="entry name" value="D-site_20S_pre-rRNA_nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="11">
        <location>
            <begin position="10"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="10">
        <location>
            <begin position="307"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="379"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="443"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="162"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="197"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="225"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="396"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="453"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR037125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR037125-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA17985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="477" mass="53752" checksum="4FB90063A4B31285" modified="2016-10-05" version="1">MDTEKHVELLVLDATPLITQSASALQQYAVRHFTTPGVKAELRDDNARNQLLLWGDKLEVRQPKQVFIDKVSAFAKLTGDYSVLSMNDIHIIALAYELEHEKNGDATLRKFPGEKLGGDDEKEKLHKRKPRFQKNEAEDSKQDAGEAQEDVEQDDDGFQVVTRKHRKTSWKQRRRPQNSHVEAKAPVTEQVNQAVQKSEHSISEPSEENKDIESSNETPQPQDFDNVEAADETGDGDSSEEDDDGDWITPGNLQETIMKDKNETVRDAPIEKDAQIAVAITTGDFACQNVSMQIGLKLMNHLSGKQIKSVKNFMYRCHACFKMTPMHKLGERKHFCPKCGGDTMIRCAVSIDNVTGKITPHLKKNFKWITRGQVYSMASPLSKNTQKKQGNGGHQHNKENRHKDLQEPVILREDQREYADAIKEDAWRRRKSEKMLQEFVGGGSADNIISPFGASNRSSGVRVGRGRNANAAKGKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H212</accession>
    <name>A0A1A0H212_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Myosin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_80073</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709357.1">
        <property type="nucleotide sequence ID" value="XM_018859167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H212"/>
    <dbReference type="STRING" id="869754.A0A1A0H212"/>
    <dbReference type="GeneID" id="30032143"/>
    <dbReference type="OrthoDB" id="4089780at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026859">
        <property type="entry name" value="Myosin-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12632">
        <property type="entry name" value="Vezatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Myosin-binding" evidence="6">
        <location>
            <begin position="187"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="584"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="584"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA17962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="768" mass="86729" checksum="9E5FB0740E291D92" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H241</accession>
    <name>A0A1A0H241_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709418.1">
        <property type="nucleotide sequence ID" value="XM_018858236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H241"/>
    <dbReference type="GeneID" id="30031212"/>
    <dbReference type="OrthoDB" id="4076141at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291652" evidence="1">
        <location>
            <begin position="19"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="247" mass="28303" checksum="AFF85EAED42F6C39" modified="2016-10-05" version="1">MLWIILFLVSLAHGLLVAQKPIHKVSTKTHDAKLVSTMLYGFYSEAYNLRDNANQHYILNFGSIALQYTSDPYCVEKSNIRYFIKSFSLVHHEYPASPCSLNLNTRFAEVVEPSPSFHVSVHGTLNRLSSELLQKSETYIKDLPDFDFHFYDFTDLNYDLQCESGYHQLMQVIFQEKVLQVNLQVLVTVPEQCTFNKLNPEFLTVFEAAKAVVHIPLSGIFYCQSGKTASCKKTVANTKNPRLKLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4F7</accession>
    <name>A0A1A0H4F7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13723</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709494.1">
        <property type="nucleotide sequence ID" value="XM_018854417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4F7"/>
    <dbReference type="GeneID" id="30027393"/>
    <dbReference type="OrthoDB" id="4084909at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="231" mass="26275" checksum="A40E50C689EBECF7" modified="2016-10-05" version="1">MGLTSDALLNRIAALELHVGIGEHWTKSAKPPLAEDKSLVSRLANLQAKVDRIFIENPELHGLQEIIQTYREDPDNYHPSVLNTTESHQNSDTVINEPRDPEDSVQVRGGFSLDETTMVNAEKQELILISYPTIIECYNLLSQFHLMDIPTLSTELTEKLSLTRLSSERLTCQTIAWQFHLLVVKSIIVIEEFAAMIKHQTLISQSIAKRMQILASKISSLERKRQLENKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4H7</accession>
    <name>A0A1A0H4H7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_47576</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709514.1">
        <property type="nucleotide sequence ID" value="XM_018858190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4H7"/>
    <dbReference type="STRING" id="869754.A0A1A0H4H7"/>
    <dbReference type="GeneID" id="30031166"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050295">
        <property type="term" value="F:steryl-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904462">
        <property type="term" value="P:ergosteryl 3-beta-D-glucoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000174">
        <property type="entry name" value="YIR007W-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041036">
        <property type="entry name" value="GH5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052066">
        <property type="entry name" value="Glycosphingolipid_Hydrolases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31308:SF5">
        <property type="entry name" value="ERGOSTERYL-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18564">
        <property type="entry name" value="Glyco_hydro_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="4">
        <location>
            <begin position="122"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5 C-terminal" evidence="5">
        <location>
            <begin position="798"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA18979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="922" mass="104998" checksum="7CA55CC10A13D548" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4J3</accession>
    <name>A0A1A0H4J3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_33621</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709529.1">
        <property type="nucleotide sequence ID" value="XM_018856354.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H4J3"/>
    <dbReference type="GeneID" id="30029330"/>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056843">
        <property type="entry name" value="THADA-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056842">
        <property type="entry name" value="THADA-like_TPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25150">
        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="3">
        <location>
            <begin position="223"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="2">
        <location>
            <begin position="626"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="4">
        <location>
            <begin position="820"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA18994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1367" mass="156593" checksum="4978EB4005D7D635" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4R5</accession>
    <name>A0A1A0H4R5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor CWC15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_47358</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709604.1">
        <property type="nucleotide sequence ID" value="XM_018858155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4R5"/>
    <dbReference type="STRING" id="869754.A0A1A0H4R5"/>
    <dbReference type="GeneID" id="30031131"/>
    <dbReference type="OrthoDB" id="30179at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006973">
        <property type="entry name" value="Cwf_Cwc_15"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12718:SF2">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04889">
        <property type="entry name" value="Cwf_Cwc_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="193" mass="21684" checksum="CC2928A4F1556195" modified="2016-10-05" version="1">MTTNHRPTLESKRGRDVAIKNTIQHARSGPGQKSLKLRLDISGAQIDHVRGRKALDELERETKRHKTLDEFLNTGSFSQVEGDGYESFDQSSSEELLSVAKCSDDDDSDEDDTNALMEELRKVREEKKSCAKQNTALISNPLIASDGSDTVNKKTSWRSSAIFSKAKKVPDQSFTTDTLNSDTHKQFLAKYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H505</accession>
    <name>A0A1A0H505_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="7">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_47469</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PPP phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709565.1">
        <property type="nucleotide sequence ID" value="XM_018858170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H505"/>
    <dbReference type="STRING" id="869754.A0A1A0H505"/>
    <dbReference type="GeneID" id="30031146"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090029">
        <property type="term" value="P:negative regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF423">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE PPQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="369"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="556" mass="61797" checksum="3C7B9DB0CCBA4042" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H587</accession>
    <name>A0A1A0H587_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709607.1">
        <property type="nucleotide sequence ID" value="XM_018858178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H587"/>
    <dbReference type="GeneID" id="30031154"/>
    <dbReference type="OrthoDB" id="3999982at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="114" mass="12502" checksum="21C13F8956E8D07A" modified="2016-10-05" version="1" fragment="single">ESNMETRKGLLTKLAGFSAVAIGLTVVGKKHIASAKEKRHSKESDASLDSDLDSEFLDNGVRPGFPKNNPNLNYESAQRESQYVGAGVAYLTRTKGDRLSLWNVIKSKMSDNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B4</accession>
    <name>A0A1A0H5B4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709806.1">
        <property type="nucleotide sequence ID" value="XM_018858093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5B4"/>
    <dbReference type="GeneID" id="30031069"/>
    <dbReference type="OrthoDB" id="2415936at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="1">
        <location>
            <begin position="122"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="403" mass="45459" checksum="0EAA789B45909280" modified="2016-10-05" version="1">MQQDTPCKSHGIPNRYNLFRFSQSEPEKKVAKKTRLKEKINNVILEYLVQEGYQSAALKLAEEASISKPNSELSSGEGYELLELDYPQIPSDVSKGLSNPGQSFTAFQGKGPEIGYVRGFSSIEIRRQLKFLILRGDISQAIRVISACYPTILDANNLLLFKLLRLNLVEMIRSHKSTSDRPLSENHLSIDEDSEKKFLNDILTFVRENLLNKVTHSMDLLRQLEVTMSLLCFDFDHKKPSNQITELPEELRKLLDLSHRNECYRAVNKVILDLDRTVQSDVLYRGVSLEAVSLSLTQVDGLAQNYEDVDDLDSSDIELNPGLDVVSSIQSMISSVPETSTQAEPVVAATDEDCKSSSEEAYSHSKLEEIAKLWLATELILFLDNLIPKKRYLQDSTGGNQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5C4</accession>
    <name>A0A1A0H5C4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_13539</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709768.1">
        <property type="nucleotide sequence ID" value="XM_018854386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5C4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5C4"/>
    <dbReference type="GeneID" id="30027362"/>
    <dbReference type="OrthoDB" id="206700at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045913">
        <property type="entry name" value="TBC20/Gyp8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913:SF7">
        <property type="entry name" value="RE60063P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20913">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 20/GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="538"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="63"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="635" mass="70936" checksum="3C68990A83182A69" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F6</accession>
    <name>A0A1A0H5F6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_46439</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM170 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709707.1">
        <property type="nucleotide sequence ID" value="XM_018858039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F6"/>
    <dbReference type="GeneID" id="30031015"/>
    <dbReference type="OrthoDB" id="2131401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071464">
        <property type="term" value="P:cellular response to hydrostatic pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019334">
        <property type="entry name" value="TMEM170A/B/YPR153W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22779:SF6">
        <property type="entry name" value="SD17342P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10190">
        <property type="entry name" value="Tmemb_170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="153" mass="16662" checksum="C35DF9D94CC68187" modified="2016-10-05" version="1">MVFISSLNVPLGYETPLFPGLYWALSGAPAKYLYYPHDIWVFTIYWALIFFLAAYIIVGCGCAASMALRRSRMPKLVVRTGVFAGLEPLLVAALYALMGAVQGAIAGTIIGVILAILYRAGSLTMNVWVPFCWGLAAILYHISSSYSTSLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5L6</accession>
    <name>A0A1A0H5L6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_13631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in ribosomal function.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709868.1">
        <property type="nucleotide sequence ID" value="XM_018854400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5L6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5L6"/>
    <dbReference type="GeneID" id="30027376"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001436">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="65"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="292"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="368" mass="41190" checksum="DB629BD8A3B84C80" modified="2016-10-05" version="1">MGILEKIAQIQEELSRTQKNKATEYHIGLLKGKLARYRRELLEPQPGQGSSGGGQGFEIAKAGDARVSLIGFPSVGKSSFLLKVTNTKSEAANYEFTTLTSVGGILEYNGAEVQIVDLPGIIKAASQGKGRGRQVIAVSRTSDLILMVLDATKSGEQREILENELESMGIRLNKEKPNILLKIKKTGGVKLNATTPPKYLDEKIVSALLKDYKIHNADVLVRDEDCTIEDFIDVINEQHMSYIKCLYVYNKADVVSLEECDRLAREPNTMVISCELELGIDELKEEIWRQLGLIRLYTKRRGIMPKFDDPMVVRRDSTILQVCDSIHREMKNQFKYALVWGSSAKHSPQKCGLQHPVNDEDVVQIVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Q4</accession>
    <name>A0A1A0H5Q4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_79767</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709769.1">
        <property type="nucleotide sequence ID" value="XM_018859123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Q4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Q4"/>
    <dbReference type="GeneID" id="30032099"/>
    <dbReference type="OrthoDB" id="10260946at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057544">
        <property type="entry name" value="Beta-prop_SPT8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23798">
        <property type="entry name" value="Beta-prop_SPT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Transcription factor spt8 beta-propeller" evidence="3">
        <location>
            <begin position="141"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="300"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="367"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="602" mass="67019" checksum="16D3D86FEB0332BB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5V2</accession>
    <name>A0A1A0H5V2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_33575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709943.1">
        <property type="nucleotide sequence ID" value="XM_018856336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5V2"/>
    <dbReference type="GeneID" id="30029312"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="52" mass="5925" checksum="8B86BF5D57D13CF4" modified="2016-10-05" version="1">MEMAPRSNPYRSIPFGLLQTSTEGEALILMKASFEKVCPGTQNFGFFHYPQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Z2</accession>
    <name>A0A1A0H5Z2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE affecting a late Golgi compartment protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_46068</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709979.1">
        <property type="nucleotide sequence ID" value="XM_018857988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Z2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Z2"/>
    <dbReference type="GeneID" id="30030964"/>
    <dbReference type="OrthoDB" id="546861at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21444">
        <property type="entry name" value="SNARE_NTD_Tlg1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048036">
        <property type="entry name" value="Tlg1p-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF224">
        <property type="entry name" value="HL02043P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="13">
        <location>
            <begin position="137"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="229" mass="26373" checksum="40170ACB47397E49" modified="2016-10-05" version="1">MDPFNEVEEDCWAQIHALQSLVSSTKQITGERKLDFQNDYQELAETLEDLRQAVHISELNPAQFSLTSSDIADRKQILAHLQKKIHDLNDTWNELVTNPHRPREVTTMSNRISQEGSENPFADDLRLDREFDLFQQQEVIQSQDLQLDLIHKTMQNLNQQAALMGNELEDQGYMLDDLDNEMDAVGNKLQRGLRRVNHVIERNRETASNWCIGILVVVLCILLVVLIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H631</accession>
    <name>A0A1A0H631_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Centractin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Actin-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Actin-related protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_216535</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the actin family. ARP1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710072.1">
        <property type="nucleotide sequence ID" value="XM_018855090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H631"/>
    <dbReference type="STRING" id="869754.A0A1A0H631"/>
    <dbReference type="GeneID" id="30028066"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10216">
        <property type="entry name" value="ASKHA_NBD_Arp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000007">
        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000018">
        <property type="entry name" value="Actin-like protein (Centractin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00083222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="47291" checksum="41D3AD867A3B03CB" modified="2016-10-05" version="1">MEEGLLYNQPIVIDNGSGNIKSGFAGEEKPRSFAPSFVGRPKYQKIMPGSLLSAIEENALKYNNTHTYIGDVAQQHRGLLRLSYPLEHGVVTNWDDMERVWHYTYSRHLKAAPEDHPLLITEAPLNPRANRDQMCQVLFETFNVPCVYVSLQAVLSLYASGRTTGVVVDSGDGVTHIVPVYDGFSLPSSIKRMDVAGRDITENLAFNIRRMSGVSLQSSSELDIARIIKEKCCYVSKDPQRDEKMYHGVPYSHYLSPGVAGGISHEGSDLFTSYKLPDGHSLNLGVEQFRAPEILFNPQLIGSESPGIHELTALAISKTDLDLRPELYQNIILSGGTTLLKNLGDRLISELKNQQSILTNTIWGSRVGTAKDSRMKIKIYAPPERKYSTWIGGSILAGLSTFRKMWVTAKQYEEDPDVVHRKCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6B4</accession>
    <name>A0A1A0H6B4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">ER membrane protein complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_33141</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The EMC seems to be required for efficient folding of proteins in the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EMC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709975.1">
        <property type="nucleotide sequence ID" value="XM_018856236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6B4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6B4"/>
    <dbReference type="GeneID" id="30029212"/>
    <dbReference type="OrthoDB" id="6745403at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045050">
        <property type="term" value="P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008568">
        <property type="entry name" value="EMC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13116:SF5">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010045">
        <property type="entry name" value="DUF850_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR010045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="257" mass="29005" checksum="CC0C49E9BE59D1A4" modified="2016-10-05" version="1">MGTETLLLDPQLKWWMLLPISVAMVLVGILRSNITFLLTLKRKALPVKAAREQDFLKRAIAFKTNAGILTSSEFETRQQYLIKSLRSTDFWEKVEEPNKLANPLVDPTTNDALMNMAKGNIMSYVPQTLIMGWVNYFFVGSVVMKLPFPLTDGFKSMLQNGVNTPNLDPRYVSAISWYFVNLFGLRPIYSLLMADPKAARDIVAQQQQQQQVMPNLGGAGGPKVEKVFKAEAESIQILDHEYVLAGITQRILEKYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H701</accession>
    <name>A0A1A0H701_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WKF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_58436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710260.1">
        <property type="nucleotide sequence ID" value="XM_018858302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H701"/>
    <dbReference type="STRING" id="869754.A0A1A0H701"/>
    <dbReference type="GeneID" id="30031278"/>
    <dbReference type="OrthoDB" id="10261563at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019327">
        <property type="entry name" value="WKF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22306:SF2">
        <property type="entry name" value="CHROMOSOME 7 OPEN READING FRAME 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10180">
        <property type="entry name" value="WKF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WKF" evidence="2">
        <location>
            <begin position="94"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="218"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="312" mass="35221" checksum="C8C68D73DE73693F" modified="2016-10-05" version="1">MGSPTILFNPTANMSKVPAWKRLGLKVKSEVTDDTFSATTHFDGSAITNKVAKKLNKKRQLEEDKNDKTKKPPKRVKKPKTERGPPPEKDQLLYLRQFVLDKENWKFSKQKQNWLLKNIESVPDSYENDLIAYFEEIQGGARTRVETDLRAVIDKWNDYSKRLEEKINAELYGGKAENAESKSEEGAKENVSKTETEAAPSKEYALRCKKLLSAMLDEPVHVEGEDQDLAAPDTPEPSNTESKEPSLASDSKIEPEAEDNLIIEDVESLGAGQEPSADNHQDVMEADKQGSKTETKQNKKDKKSKKEKKTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H743</accession>
    <name>A0A1A0H743_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_45051</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710243.1">
        <property type="nucleotide sequence ID" value="XM_018857870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H743"/>
    <dbReference type="STRING" id="869754.A0A1A0H743"/>
    <dbReference type="GeneID" id="30030846"/>
    <dbReference type="OrthoDB" id="184583at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006404">
        <property type="term" value="P:RNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="416" mass="45558" checksum="C783EDCCF24CA55B" modified="2016-10-05" version="1">MAALNKDVDVGVTESTTFSIAGKQIKFNSAEDIAPYVQQLAAQDGVRKIDISGNTIGIDASEVFAAAILKHKTTLVEVDFSDIYTGRLNTEIPQSLQHLLPALLQCADLKVVNLSDNAFGLQTIDPIEEYLAKAVSVEHLILSNNGMGPFAGARIGKSLFQLSLAKKQQKYPSLKTFICGRNRLENGSINYLAIGLRAHEHLHTVRLYQNGIRPAGIEKLILQGLARNSHLQVLDLQDNTLTTRAAIALAELLSAWDSLVELNLNDCLLKPKGSLALADHLANGSVKSKFETLKLQYNELEADALKRLVRAVSEKLPHLKTLELNGNRFDEEAEEIDAFNSVFEARGHGELDELDELEELDSDEENEDDDEDDEDTFDGAAADLNQLEAELAGFSVEDKDSTVDSIADELAGTHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H780</accession>
    <name>A0A1A0H780_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">OLA1</name>
        <name evidence="6" type="ORF">METBIDRAFT_12976</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710474.1">
        <property type="nucleotide sequence ID" value="XM_018854288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H780"/>
    <dbReference type="STRING" id="869754.A0A1A0H780"/>
    <dbReference type="GeneID" id="30027264"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="22"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="31"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="396" mass="44340" checksum="BBF53304CC05B4F4" modified="2016-10-05" version="1">MPPKKKGVVEKPILLGRPGNNLKSGIVGLANVGKSTFFQAITKSPLGNPANYPFATIEPEEARVIVPSARFDKLCEMYKPKSEVPAFMTIYDIAGLTKGAHAGEGLGNNFLANIRAVDAIYQVVRCFEDADIIHINDEVNPINDLEIIKDELRLKDIEFAQKHLEGIEKITKRGGQSLEVKQKKEEAELVKSIIELLETGGRVANQKWTQKEVEVINSMFLLTAKPCIYLLNLSERDYVRKKNKWLLKIKEWVDANSPGDVVIPLSVSMESHLATLETDEEREAYIKEVGAGSAFPKIITTMRNKLDLISFFTGGPDEVREWTIRRWYTAPQAAGTIHTDLERTFILANVMKYDDFIEYGDEVAVKSAGKLMQKGKDYFVEDGDILHVKAADGKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7F5</accession>
    <name>A0A1A0H7F5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-2,6-bisphosphate 2-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_12942</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the phosphoglycerate mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.46" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710435.1">
        <property type="nucleotide sequence ID" value="XM_018854284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7F5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7F5"/>
    <dbReference type="GeneID" id="30027260"/>
    <dbReference type="OrthoDB" id="267323at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003873">
        <property type="term" value="F:6-phosphofructo-2-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006003">
        <property type="term" value="P:fructose 2,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000005">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000644">
        <property type="entry name" value="GpmB, Fructose-2,6-bisphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003094">
        <property type="entry name" value="6Pfruct_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013079">
        <property type="entry name" value="6Phosfructo_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606:SF44">
        <property type="entry name" value="6-PHOSPHOFRUCTO 2-KINASE_FRUCTOSE 2,6-BISPHOSPHATASE LONG FORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10606">
        <property type="entry name" value="6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01591">
        <property type="entry name" value="6PF2K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00991">
        <property type="entry name" value="6PFRUCTKNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-phosphofructo-2-kinase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="231"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="458" mass="52698" checksum="23FB387B9461377C" modified="2016-10-05" version="1">MPVYSVSHVENVRICVIMVGLPARGKSLIAQKIVRYLLWLSIAAKCFSVGSYRRALAGTSPISAEFFSPDNEDGIRTRGKAIDMATADMMRWFTEEHGVVGIIDATNLTRERRARLLKLCRENLIEPIFLESWCDDPDVILYNSADVKTTSPDYVDVDPNIATKDFLLRIQHYEKTYETMNVDDDKDLTFIKLVNVGSQIIVNRIESYLESRIVYYVMNLHIKTRSIWLLRHGESEYNLSGKIGGDCNLSERGWKYAKKLPEIVLKSLGEDNEHTNLTVWTSTLKRTQQTASFLPYEKKLEWKALDELDAGECDGMTYEEIEEQFPEDFKARDDNKYEYRYRGGESYRDIVIRLEPIIMELERQENILIITHQAVLRCLYAYFMNVPQEESPWMSIPLHTLIKLEPRAYLTLVTRIKADIPAVSTYKERGTSQLGELEVNTSVSRDLISNSSNETPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7P9</accession>
    <name>A0A1A0H7P9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710649.1">
        <property type="nucleotide sequence ID" value="XM_018856213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7P9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7P9"/>
    <dbReference type="GeneID" id="30029189"/>
    <dbReference type="OrthoDB" id="46189at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08700">
        <property type="entry name" value="VPS51_Exo84_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="812"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="829"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="859"/>
            <end position="869"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="953" mass="109635" checksum="95CC2505AB74FF52" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7T5</accession>
    <name>A0A1A0H7T5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lysophospholipase</fullName>
            <ecNumber evidence="6">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_13106</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the lysophospholipase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710611.1">
        <property type="nucleotide sequence ID" value="XM_018854306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7T5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7T5"/>
    <dbReference type="GeneID" id="30027282"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF56">
        <property type="entry name" value="MEIOTIC PHOSPHOLIPASE SPO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444428" description="Lysophospholipase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="7">
        <location>
            <begin position="62"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="726" mass="80912" checksum="E09AF68584D9ABE3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U8</accession>
    <name>A0A1A0H7U8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">37S ribosomal protein MRP2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_79286</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710492.1">
        <property type="nucleotide sequence ID" value="XM_018859032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7U8"/>
    <dbReference type="GeneID" id="30032008"/>
    <dbReference type="OrthoDB" id="413436at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1480:FF:000001">
        <property type="entry name" value="30S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006477">
        <property type="entry name" value="PRK08881.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836:SF19">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00076896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="115" mass="13337" checksum="AE33149707BBA39A" modified="2016-10-05" version="1">MPFRFPVKFQLPQHCYLNGRVIKDHFKRLQYAEHEVTRKALKYIARNTELPAKARLEAQLQLTAMPHYTSITQIKDRCIASGHSRAVISDFKLNRTAFRERARAGQIPGVTKASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Y7</accession>
    <name>A0A1A0H7Y7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="2">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_79320</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710532.1">
        <property type="nucleotide sequence ID" value="XM_018859037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Y7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Y7"/>
    <dbReference type="GeneID" id="30032013"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032093">
        <property type="term" value="F:SAM domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000196">
        <property type="term" value="P:cell integrity MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071507">
        <property type="term" value="P:pheromone response MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001402">
        <property type="term" value="P:signal transduction involved in filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09534">
        <property type="entry name" value="SAM_Ste11_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000334">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029458">
        <property type="entry name" value="Ras-bd_By2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14847">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00536">
        <property type="entry name" value="SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01304">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM" evidence="12">
        <location>
            <begin position="2"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="355"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="644" mass="72286" checksum="8EF04984223C1102" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H813</accession>
    <name>A0A1A0H813_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710650.1">
        <property type="nucleotide sequence ID" value="XM_018856214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H813"/>
    <dbReference type="STRING" id="869754.A0A1A0H813"/>
    <dbReference type="GeneID" id="30029190"/>
    <dbReference type="OrthoDB" id="415590at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF46">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="12"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="169" mass="19104" checksum="579876E95010C751" modified="2016-10-05" version="1">MASRPQTRSKNGCFTCRRRKKKCDESSYPSCKNCISNKLECSWPAHVVNAHKSEQDEDISTSDIVSPQKALNLSASGELKMTSLPWNSLVPDKISKPGKRDKNYILQRISMQQDCVEEQDEIPDIMDSTKSVAKLYKLTPISLIRDRIANQLDIPGDEIKENFQGNTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C0</accession>
    <name>A0A1A0H8C0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_12315</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710793.1">
        <property type="nucleotide sequence ID" value="XM_018854210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8C0"/>
    <dbReference type="GeneID" id="30027186"/>
    <dbReference type="OrthoDB" id="3598904at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052360">
        <property type="entry name" value="Transcr_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206">
        <property type="entry name" value="ASPERCRYPTIN BIOSYNTHESIS CLUSTER-SPECIFIC TRANSCRIPTION REGULATOR ATNN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36206:SF13">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN MOC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="8">
        <location>
            <begin position="348"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="385"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="403"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="429" mass="46536" checksum="14C70B52B904373F" modified="2016-10-05" version="1">MPQLPSVSELLIGAHNPAGPGVRPESQLPQSYKSVTAAQKRLSETSPRQKEVFLPSGPVKPARVLQLIHAYPGMAHGHMDGHVATAHLAKESHVGPSAMSSGRNSVLSIGLDSHVPMDCFNYRESHRPRHVLVHLQLLHLQLHLLLLLPQLHYRPRQESSAGLVTRATLYQSFGSQESRLPLGESPTFAARPRPASFSYAPGPVPAGVPGPAYNPQMTQAFHVAGPATGPAPGAPGMVIPMAFYNGATAGSGEAANYGQPVPMVGYPGYYNTVPYNAQVSPGAAVAYEHYPHAQFKAGPPYLQMEGIPAGHSSAHYQKHGVSMEEMTSDPNHALINKRCIIKRRTRTGCLTCRKRRIKCDERKPHCFNCERLKKLCLGYEALNGKDSSNENTEKERLKGGLRSDPSSPTSPSGPLPSKERHRSSVHDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C4</accession>
    <name>A0A1A0H8C4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_195093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710798.1">
        <property type="nucleotide sequence ID" value="XM_018854865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C4"/>
    <dbReference type="GeneID" id="30027841"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="187" mass="22195" checksum="FBCCE8FE61459A6A" modified="2016-10-05" version="1">MDKYRGEICHPSQFSFLLKKHRIISCLWRDARPPQTKKYKEKTHDRSQHWLRWKARAPIHEISQRRCDSLKKTSAHNQYKTLNKTTKCYCPMRHVFLLSFGPGPVDLFSLILAQVWTAVLCWRHPRFETQGNPLSAKVVLIILRLFRNTSQLFIVLPQPHEQSPNFSQKHYLCFIHQKQENETFGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8D4</accession>
    <name>A0A1A0H8D4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_197389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710896.1">
        <property type="nucleotide sequence ID" value="XM_018854887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8D4"/>
    <dbReference type="GeneID" id="30027863"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="63" mass="6933" checksum="ED710FAF30D569DC" modified="2016-10-05" version="1">MGGLKVNCQQSCIASQNRHCALLFIILCVLPFLDVMCLDVFSLVSVFHGSIFDLHRPHLTSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8N2</accession>
    <name>A0A1A0H8N2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 11">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_43211</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 11"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710765.1">
        <property type="nucleotide sequence ID" value="XM_018857628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8N2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8N2"/>
    <dbReference type="GeneID" id="30030604"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000012">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="12">
        <location>
            <begin position="4"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="317" mass="35378" checksum="9AEA397D39717208" modified="2016-10-05" version="1">MKSLLKDLHVHYVRELDSKITKQTYEYWLLEHLRLNGLYWGSMALATLGSLDTLPKDQVEQFVMSCYDEQAGAFAAFPGHDAHVLTTLSALQILLIYDRFDALSPQKKDRIAAFVLRLRLPDGSFMGDSFGEIDTRFVFVSVFILTLLDRLSPAVADSAAHFILSCRNFDGGFGMYPGAESHAAQVYTCIGALALCDALDNVEEKTAAWLSERQVVGSGGFNGRPEKLPDVCYSWWVLLSLAALDKAHWINFVLDCQDLQAGGFADRPDNQTDVYHTCFALTGLSLMFAEKYGLAEVDPVMCLPKRCVGQINYYKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8P9</accession>
    <name>A0A1A0H8P9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711016.1">
        <property type="nucleotide sequence ID" value="XM_018856059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8P9"/>
    <dbReference type="GeneID" id="30029035"/>
    <dbReference type="OrthoDB" id="4093454at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291739" evidence="1">
        <location>
            <begin position="19"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="7595" checksum="68D68CFA26DBECB2" modified="2016-10-05" version="1">MQFSTLATFAAAAASVSAFANSTVTDVTSTLITITSCESAVTNCPYTTANVSTYHGAGNKQYAAGAVALAAGALLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8R3</accession>
    <name>A0A1A0H8R3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_198031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710923.1">
        <property type="nucleotide sequence ID" value="XM_018854896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8R3"/>
    <dbReference type="GeneID" id="30027872"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="263" mass="28616" checksum="213E1408C62CA57A" modified="2016-10-05" version="1">MAAGPSAVSCRATCQKPNVLGHVSSAKSAGNLEPIPGSLSRLIVPAYCPGTGLSEYSPFAPPHPARKRPQKIGTPETRHAPERPPDDFPWRASLDPAQGRCFNSHSLHHIQPGSGHKKLEPRKPDTRRNARLTIFHGVPRWTQLRAAVSIVPMVHCQLQNVPSAWPKQARPTAKSTGGLYCRAKPQCQPAKPRPGVPSPDMPNACLRVPSISAGASCDLRILPAPPAIYEFCRIHPICRICRIYQMLLESADPADPANRSRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Z7</accession>
    <name>A0A1A0H8Z7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_197023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710879.1">
        <property type="nucleotide sequence ID" value="XM_018854879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Z7"/>
    <dbReference type="GeneID" id="30027855"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="76" mass="9209" checksum="7D3B549B45BDAC6C" modified="2016-10-05" version="1">MGFWRAPVGLYSWGVGDFLYLWWACIFRDLYLWWGSLWWGSLWWVCMYGGFVCMVGLYVWWVCMYGGFVFLVVLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9J4</accession>
    <name>A0A1A0H9J4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphodiesterase</fullName>
            <ecNumber evidence="3">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_44204</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="3">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711208.1">
        <property type="nucleotide sequence ID" value="XM_018857759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9J4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9J4"/>
    <dbReference type="GeneID" id="30030735"/>
    <dbReference type="OrthoDB" id="546632at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="4">
        <location>
            <begin position="188"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="606" mass="67589" checksum="1EAB09DA491C77D3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9R5</accession>
    <name>A0A1A0H9R5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Nucleoporin NUP188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_31535</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Nup188 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711379.1">
        <property type="nucleotide sequence ID" value="XM_018855876.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H9R5"/>
    <dbReference type="GeneID" id="30028852"/>
    <dbReference type="OrthoDB" id="102511at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018864">
        <property type="entry name" value="Nucleoporin_Nup188_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044840">
        <property type="entry name" value="Nup188"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041634">
        <property type="entry name" value="Nup188_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048883">
        <property type="entry name" value="Nup188_N-subdom_III"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31431">
        <property type="entry name" value="NUCLEOPORIN NUP188 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18378">
        <property type="entry name" value="Nup188_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10487">
        <property type="entry name" value="Nup188_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21093">
        <property type="entry name" value="Nup188_N-subdom_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal" evidence="10">
        <location>
            <begin position="39"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup188 N-terminal subdomain III" evidence="12">
        <location>
            <begin position="552"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear pore protein Nup188 C-terminal" evidence="11">
        <location>
            <begin position="1288"/>
            <end position="1606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1627" mass="180474" checksum="7AE5563759325946" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9U4</accession>
    <name>A0A1A0H9U4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TOM13-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_21877</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711409.1">
        <property type="nucleotide sequence ID" value="XM_018855112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9U4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9U4"/>
    <dbReference type="GeneID" id="30028088"/>
    <dbReference type="OrthoDB" id="5529571at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013262">
        <property type="entry name" value="OMP_MIM1/TOM13_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28241:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08219">
        <property type="entry name" value="TOM13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="58" mass="6553" checksum="991B06D6BF9CAEE2" modified="2016-10-05" version="1" fragment="single">TVVTLNIWDIVRRGAINLVLPFINGIMLGFGEILAHEIGFHYGFFGARVQPARRAEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W0</accession>
    <name>A0A1A0H9W0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Transcription elongation factor SPT5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32760</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711323.1">
        <property type="nucleotide sequence ID" value="XM_018856126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9W0"/>
    <dbReference type="GeneID" id="30029102"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001042">
        <property type="term" value="F:RNA polymerase I core binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001208">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001209">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000232">
        <property type="term" value="P:regulation of rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090262">
        <property type="term" value="P:regulation of transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06082">
        <property type="entry name" value="KOW_Spt5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06083">
        <property type="entry name" value="KOW_Spt5_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06084">
        <property type="entry name" value="KOW_Spt5_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06085">
        <property type="entry name" value="KOW_Spt5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.940:FF:000005">
        <property type="entry name" value="Transcription elongation factor SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041975">
        <property type="entry name" value="KOW_Spt5_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041976">
        <property type="entry name" value="KOW_Spt5_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041977">
        <property type="entry name" value="KOW_Spt5_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041978">
        <property type="entry name" value="KOW_Spt5_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057936">
        <property type="entry name" value="KOWx_Spt5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039385">
        <property type="entry name" value="NGN_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022581">
        <property type="entry name" value="Spt5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017071">
        <property type="entry name" value="TF_Spt5_eukaryote"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23042">
        <property type="entry name" value="KOW1_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23284">
        <property type="entry name" value="KOW2_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23291">
        <property type="entry name" value="KOW4_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23290">
        <property type="entry name" value="KOW5_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23037">
        <property type="entry name" value="KOWx_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11942">
        <property type="entry name" value="Spt5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036945">
        <property type="entry name" value="Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="9">
        <location>
            <begin position="318"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="624"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="749"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="841"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="937"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="951"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="976"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="989"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1014"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1040"/>
            <end position="1059"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1089" mass="115977" checksum="606527713A57DB38" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAE1</accession>
    <name>A0A1A0HAE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78085</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711491.1">
        <property type="nucleotide sequence ID" value="XM_018858816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAE1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAE1"/>
    <dbReference type="GeneID" id="30031792"/>
    <dbReference type="OrthoDB" id="10071381at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="6">
        <location>
            <begin position="2"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="5">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="171"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="389"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="254"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="412"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="57264" checksum="C5040E16102BEA84" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG6</accession>
    <name>A0A1A0HAG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_165171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711378.1">
        <property type="nucleotide sequence ID" value="XM_018854653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAG6"/>
    <dbReference type="GeneID" id="30027629"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="161" mass="18273" checksum="3739997B9AA723CD" modified="2016-10-05" version="1">MMVGNFWWFSSMSGACLSISIMGLGREMMSSVIGFQRAVFLQGRDLRMFWQLYSLIWLQVFFLENSKAPRKRAVLLVACLGTSPKWPGNISIMNQIRTHQLASHCFSEEENGLPAVEKLLPRNGPISSTIWERPDTAPSGDTQNMIIKLQLSIYIQRICCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAY5</accession>
    <name>A0A1A0HAY5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_41365</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711552.1">
        <property type="nucleotide sequence ID" value="XM_018857388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAY5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAY5"/>
    <dbReference type="GeneID" id="30030364"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008361">
        <property type="term" value="P:regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900101">
        <property type="term" value="P:regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000304">
        <property type="term" value="P:response to singlet oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014402">
        <property type="entry name" value="Sig_transdc_resp-reg_Skn7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF361">
        <property type="entry name" value="TRANSCRIPTION FACTOR SKN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002595">
        <property type="entry name" value="RR_SKN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="10">
        <location>
            <begin position="284"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="204"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="439"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="7">
        <location>
            <position position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="464" mass="52315" checksum="4A42B8CBAF49826A" modified="2016-10-05" version="1">MAAPKDDNPGLNGLNDFVKKLYQMLLQDRYLDVVRWTPHGTSFVVLNTNEFTKCILPTHFKHSNFASFVRQLNKYDFHKVKISSEQRQAYQYGDDAWEFQHPDFKKDDTDALENIRRKAPTKKEPPQDAGQLQAQVLQLRTDVAKLQNDLRVLNEKYRAMSDSVVAHHTFNERQYRSMGMLVHCLTQAGIKVPPLDLPNPSLLSMVQPPLARDKPPAHHQHQTIQTHQQRQQREQQKLQAPAAPGPASRLEPAHLRQHSTDGAAVPLAKYEPVSTTLMPNAKFHVLLVEDDNVCIQLCRKFLMKYGCQVTVVTDGLNAISTVEHTKYDLVLMDIVMPNLDGATATNIIRSFDTQTPIGPKLEFLDLPPDNDLVNYLRNGMSDILAKPFTKDDLYLILSKHLLGPKLEFLDLPPVPDFAGLTPKGPLQVAMPQAIPNITPTPPLGQGIIDPMDTLEPSAKKPRMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB60</accession>
    <name>A0A1A0HB60_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_11893</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711874.1">
        <property type="nucleotide sequence ID" value="XM_018854121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB60"/>
    <dbReference type="STRING" id="869754.A0A1A0HB60"/>
    <dbReference type="GeneID" id="30027097"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="215" mass="24148" checksum="A9D4B19DD1CBFB94" modified="2016-10-05" version="1">MQKFRKRTFTAVSAQIWQQLTGMNIMMYYIVCIFQIAGYNGNTNLVPSLIQYILNMAVTIPALFTSDLLGRRTILLAGAALMMAFQFGVAGILATYSEPVFISDTVRITIPEENKSATKGVIACCYSFVPLHLSGALESGFIAGRYIGGMGRLAIQKTWSFTFHLCKLDLQFCHFHVHVFIIRNITWKAYCIYATFCAFAELIQKLEISHEGNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBB3</accession>
    <name>A0A1A0HBB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_69404</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711677.1">
        <property type="nucleotide sequence ID" value="XM_018858377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBB3"/>
    <dbReference type="GeneID" id="30031353"/>
    <dbReference type="OrthoDB" id="3361892at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032931">
        <property type="term" value="F:histone H3K56 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051236">
        <property type="entry name" value="HAT_RTT109-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016849">
        <property type="entry name" value="Rtt109"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31571:SF2">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE RTT109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51728">
        <property type="entry name" value="RTT109_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="440" mass="50413" checksum="F4581FF462CDF466" modified="2016-10-05" version="1">MDLSSVLPEGLTYTALCFRTNAYQCKPLLISREKPLTLKVKHMFLLLEKESNVIIFGIEVYVYLTFHGKKLERHVFILKADTTGLGNHRISMASVTKCFLRYLISINPKVYLDKATYRSSNNNHNALHSNELLSTINMIIQDLRAWSRKLKQNPHLITDFLRKDDDNGIHPSSDLPVIMPVSMSSKISLFTRSADAYLFPESQNNKGKHNINGNTLFKWWISILKDTLDQSWVCKVDIPGSDHVSVRKFLPEASNWDIGNIHVSDRLKDRAVYTIPLLPDDPKGRFLEHLIVENRYKSLTTKQFWNELGFRQEFRLGNVVGIIGCFKECESLEGTIEDPRTSLLRPRQYKKIREVIKGENYNQINDVLNLWSSGIPELAARLKLSLRFVSILGTLSSVDNVDHGKENLRKVLSLVNVLNVKKKSAPVNNLNTLVKRKKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBH8</accession>
    <name>A0A1A0HBH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YAE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein yae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_27045</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711742.1">
        <property type="nucleotide sequence ID" value="XM_018855329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBH8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBH8"/>
    <dbReference type="GeneID" id="30028305"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062092">
        <property type="term" value="C:Yae1-Lto1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038881">
        <property type="entry name" value="Yae1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829:SF0">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="8">
        <location>
            <begin position="48"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="128" mass="14221" checksum="0E14914324766013" modified="2016-10-05" version="1" fragment="single">CTGKCHCKAKKSEKTSDKDLQLTDDVWGSDEESISIHADLQRAHVNQGYLDGITKAQELGLQDGFDKGFPTGASLGIRVGKVLATLHGKSQFKEAQGALNITQVLDKKYFDNQLDLHNSQHLLLQRWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBP3</accession>
    <name>A0A1A0HBP3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711807.1">
        <property type="nucleotide sequence ID" value="XM_018855930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBP3"/>
    <dbReference type="GeneID" id="30028906"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8580" checksum="2F71A4D8FDF0533C" modified="2016-10-05" version="1" fragment="single">MSEILVSNISDDGWKVQGRKRRNSRKRPERSVIMATTTGSVLTDQKGSLGSNPSMRQPSSNLNPFLVLAEISEEDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBP5</accession>
    <name>A0A1A0HBP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_182818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711915.1">
        <property type="nucleotide sequence ID" value="XM_018854786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBP5"/>
    <dbReference type="GeneID" id="30027762"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="112" mass="12711" checksum="680584673B82EC91" modified="2016-10-05" version="1">MVNLLGLLAAVYSVVLCRCVLRWSYAEVYFGDLLILVCFDLFPMVYLGTLVVICHQWSAVVERNHRCTGHGLGSPFILPDMGARSISMQNRKKIPGRPKADVPWCRNVHIMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS2</accession>
    <name>A0A1A0HBS2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Protein transport protein BOS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_11871</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">SNARE required for protein transport between the ER and the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 12">Belongs to the BOS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711848.1">
        <property type="nucleotide sequence ID" value="XM_018854116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS2"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS2"/>
    <dbReference type="GeneID" id="30027092"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027027">
        <property type="entry name" value="GOSR2/Membrin/Bos1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230:SF1">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21230">
        <property type="entry name" value="VESICLE TRANSPORT V-SNARE PROTEIN VTI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028865">
        <property type="entry name" value="Membrin-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR028865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="225" mass="26159" checksum="22851885055BAAE3" modified="2016-10-05" version="1">MNSIYNHGVRQTQMVTRDLAAFEKNLSTSPMSLQGTIITSLTAFKKTIKEYGDLMNQNNFTEEKHKIRLEKFRADLEQFQLKFDSLRKQREVLLQAESRQELMGRRTVNSDNPYGQTTDPQSGMAQQAQMGYSEGLYKEKQSLGRGSQQLDMILEMGQSTLGDLVDQNETLKRLGSKFEESLMTLGVSQGTIRRVERRAKQDKWIFWSLVVLFFVLCYFIMKWFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBU0</accession>
    <name>A0A1A0HBU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_83176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712114.1">
        <property type="nucleotide sequence ID" value="XM_018859228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBU0"/>
    <dbReference type="GeneID" id="30032203"/>
    <dbReference type="OrthoDB" id="4096151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508819" evidence="2">
        <location>
            <begin position="29"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="460"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="492"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="550" mass="59911" checksum="2510269412419329" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC29</accession>
    <name>A0A1A0HC29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histone transcription regulator 3 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_83144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712076.1">
        <property type="nucleotide sequence ID" value="XM_018859227.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HC29"/>
    <dbReference type="GeneID" id="30032202"/>
    <dbReference type="OrthoDB" id="77564at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22249">
        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033053">
        <property type="entry name" value="Hir3/CABIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15502:SF7">
        <property type="entry name" value="CALCINEURIN-BINDING PROTEIN CABIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1732"/>
            <end position="1761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1943"/>
            <end position="1978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1978" mass="227892" checksum="D2D90542931850AB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG7</accession>
    <name>A0A1A0HCG7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32097</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712113.1">
        <property type="nucleotide sequence ID" value="XM_018855984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCG7"/>
    <dbReference type="GeneID" id="30028960"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004711">
        <property type="term" value="F:ribosomal protein S6 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="109"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="9">
        <location>
            <begin position="376"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="51712" checksum="674DBA0396774952" modified="2016-10-05" version="1">MTEIFPMDGYEAELVAGVSSLNYRLLEDKHADLEEPVSCPKTDSEPSDQDSNDSDVVTELLARPVPAQSRSRRRSSVSKYSSSYELDIHELFVSLTLVASGHRPKLADFEPIKVLGHGSYGKVLLVREKSSGRLFAQKQLKKALLVINETNEVHQKNYERTINEKAILEKVNHPNVVKLYYAFQDNNKVYLILEYLDGGEIFHHLAQQRYLSEKDASFYIAQIVLALRYLHSSLKVIYRDLKPENCMLNSAGHLVLTDFGLSKFSSDDADANHLMIGTAQYMAPEVIKGEDYDFRVDWWSLGCVAFDMLTGGPPFTGHTNDKIMDKIVHSKKHLKFPFYLSHDAKDLLRKLLQPSAAKRFDVDVDYEEFKQHRFFRHIDWKVIEQGCAGDVLPPILPVITDPILAENFDEEFTTMQFTPPAHDPGVSRLPDEVLHVKGFTFTNESFLASVLGAGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCL2</accession>
    <name>A0A1A0HCL2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SWIRM-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32189</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712231.1">
        <property type="nucleotide sequence ID" value="XM_018855999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCL2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCL2"/>
    <dbReference type="GeneID" id="30028975"/>
    <dbReference type="OrthoDB" id="5598695at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000087">
        <property type="entry name" value="Transcriptional adapter 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF21">
        <property type="entry name" value="SWIRM DOMAIN-CONTAINING PROTEIN FUN19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="2">
        <location>
            <begin position="240"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="38562" checksum="93160DE21F508340" modified="2016-10-05" version="1">MTLLYQPISRTFGQRDGCLTPVNNQDDKRSSLKSIQNSAISNTLSSDFSSTHKIISPPLSPYQRESSSHDANDDIDAASDTEHYLASNNPFKIENFKSYSMTVDPWTKLDSRYKQRQLDFLSQYSFEAPRVVYLHVLRLSERRVERRKRVAQPTSSDSESDSIGVRTRKHAKESHAEVLTKEVDADVHSSPPQKKRKVRKEASYAGSPLAAQQAALIDESIPDYSPDAHATLPKGNTRALKIEWKGQPMDLRNDPNLDKLHPAEVVLASTLRLRCNVFLDSKRRFFYEKVNRLRNNMPFRRTDAQKACRIDVNKASRLFAAFEKAGWLDDKHFEKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCP7</accession>
    <name>A0A1A0HCP7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41916</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712198.1">
        <property type="nucleotide sequence ID" value="XM_018857465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCP7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCP7"/>
    <dbReference type="GeneID" id="30030441"/>
    <dbReference type="OrthoDB" id="275582at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000614">
        <property type="entry name" value="Mrp20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="287" mass="32702" checksum="60D6324FEAB499FA" modified="2016-10-05" version="1">MDAFRSSVLRLVIALRSIRHKPKASAYPKVNVQDVVLDRPRYGFRKVLPPLLSQSVQNEVFPAEAIRAKYLAAGKPVPLKFHNKSDAIARRNYDLYKELVALGLPHFRVGEKKVYLPKARVCLLRPNAKHTPYQAKFLVPRNFNKMDLRDYLWHVYGLRALNVTVQLLHARFMRNPSDLARYRGPQYKKMTIDMEEPFVWPALPDGLETGARQTMADQMSVTKMLMPVGSEKNLVNDSFDGLYAKPQLPDIFVSKKMQKALRPAVALALDTAQAQSDRAKVAHLLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCW8</accession>
    <name>A0A1A0HCW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_39619</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712357.1">
        <property type="nucleotide sequence ID" value="XM_018857158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCW8"/>
    <dbReference type="STRING" id="869754.A0A1A0HCW8"/>
    <dbReference type="GeneID" id="30030134"/>
    <dbReference type="OrthoDB" id="415015at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="10">
        <location>
            <begin position="168"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="564"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="604"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="636" mass="72884" checksum="69629ED187DC9463" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD42</accession>
    <name>A0A1A0HD42_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_77519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712495.1">
        <property type="nucleotide sequence ID" value="XM_018858714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD42"/>
    <dbReference type="STRING" id="869754.A0A1A0HD42"/>
    <dbReference type="GeneID" id="30031690"/>
    <dbReference type="OrthoDB" id="5563539at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24816" checksum="E20101310F0D1EB7" modified="2016-10-05" version="1">MDDCFIHRRSLFSENVDYMSPNNISNFDLHLCWKENNSVSVKSSQDDISSKIPSSVSRRLENVSWRRMYKQIWNLSEALPATINWSKSLDITWLFGPKYGVDSPLADVPLTSSNLSKLTRDDILECAADEVSSMSSTHLMSFDDRSLMELLEDIGEEYDECCSGLKLVLKLECRRFVRQEKNTKKRVKFNYIINSREIMNGISFDYHFLDPLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD62</accession>
    <name>A0A1A0HD62_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CinA C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_77451</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712406.1">
        <property type="nucleotide sequence ID" value="XM_018858704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD62"/>
    <dbReference type="STRING" id="869754.A0A1A0HD62"/>
    <dbReference type="GeneID" id="30031680"/>
    <dbReference type="OrthoDB" id="2350783at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="CinA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA C-terminal" evidence="1">
        <location>
            <begin position="12"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="175" mass="18708" checksum="6C2795D3026FA96E" modified="2016-10-05" version="1">MSAFPPASIKLLVEEIATILRDRGQTLAVSEAACGGLLLAYIVSVNGASNFYIGGKLVYLVKQRLKLSGWTDDEIHSYVGPLEQVALKLARTTKYELGSTYVLSETGYAGPTTDLHLAGHGDHGQVGTVFLGLSGPCGDISIRHETGLGDRSANMELFARLGLEFLLDQLKSLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE12</accession>
    <name>A0A1A0HE12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="2 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_39899</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-formylglutathione + H2O = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the esterase D family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="2 7"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712835.1">
        <property type="nucleotide sequence ID" value="XM_018857199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE12"/>
    <dbReference type="STRING" id="869754.A0A1A0HE12"/>
    <dbReference type="GeneID" id="30030175"/>
    <dbReference type="OrthoDB" id="420518at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000002">
        <property type="entry name" value="S-formylglutathione hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061:SF0">
        <property type="entry name" value="S-FORMYLGLUTATHIONE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="287" mass="31925" checksum="B79B700D38E57742" modified="2016-10-05" version="1">MSFTTNAEIGQFGGKLYKLSHDSAATNCKMEVNVYIPGPEPRRVPVLLYLSGLTCTPDNATEKSFLAYFAAKFGFALVFPDTSPRNTGIPGEDDEHDLGSGSGHYLNATQQPWARHYNMFSYVHEELLDKLASRFPQLDMARISLTGHSMGGGGAISGFLKNPGKYRSVSAFAPLSNPLNAPLGTKNFEAYLGRDTTLWRQYDPVYLIQEYTHEHQPDILIHQGAQDEFWAEEGTLQPENLVKAAEDSSYRGKVDLRIVEGYDHLYFFVSSFAEEHAVHHAKALGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE59</accession>
    <name>A0A1A0HE59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_86898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712771.1">
        <property type="nucleotide sequence ID" value="XM_018859269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE59"/>
    <dbReference type="GeneID" id="30032244"/>
    <dbReference type="OrthoDB" id="4012145at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="141" mass="16285" checksum="CE9F2E308A1D91B3" modified="2016-10-05" version="1">MVYGSRVPNYRRSKTFAIMSFLPHIKLTMPSASGFILSTVLGLAARRLQIQIVGKPTVASWNSVQGYALSVSAFTTGYLICEHFVERNRQLLTRRLLQLREQRSQLNSFQDFDLDAEHRLTAQMRTSRFFELFEKMGEAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE78</accession>
    <name>A0A1A0HE78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pep3/Vps18 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_40315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712704.1">
        <property type="nucleotide sequence ID" value="XM_018857257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE78"/>
    <dbReference type="STRING" id="869754.A0A1A0HE78"/>
    <dbReference type="GeneID" id="30030233"/>
    <dbReference type="OrthoDB" id="1845386at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048284">
        <property type="term" value="P:organelle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006904">
        <property type="term" value="P:vesicle docking involved in exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007810">
        <property type="entry name" value="Pep3/Vps18_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23323:SF26">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 18 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05131">
        <property type="entry name" value="Pep3_Vps18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pep3/Vps18 beta-propeller" evidence="4">
        <location>
            <begin position="19"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1062" mass="121121" checksum="2A826F1A46DF3E90" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE90</accession>
    <name>A0A1A0HE90_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription initiation factor IIA large subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_39319</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712713.1">
        <property type="nucleotide sequence ID" value="XM_018857128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE90"/>
    <dbReference type="STRING" id="869754.A0A1A0HE90"/>
    <dbReference type="GeneID" id="30030104"/>
    <dbReference type="OrthoDB" id="6275927at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
        <property type="entry name" value="TFIIA_alpha_beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.100:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01371">
        <property type="entry name" value="TFIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="164"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="172"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="253" mass="28544" checksum="14F6162CC897E9FC" modified="2016-10-05" version="1">MSNFEAGKLYEAVVEEVIADSRQDFEDSGIDESTLQELKKLWCDKLSQFKVCRFSWDDENDERHHIQEGVIDESTMKSEPLQLNANLDLPIIPNFQPSTSIPSLDLTNGSYSMGIELPSVKQEIDEGGLVLPANQSDGPFEMTISLPQNRAQSYLSRLRENMKAQADGPGSLDDDDDDDVFNDSDDINSDLDDDLDSEKSDEEDGDQEGQIMLCLYDKVQRVKNKWKCNLKEGVANIDGRDYVFQRAAGESEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEM5</accession>
    <name>A0A1A0HEM5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_152444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712859.1">
        <property type="nucleotide sequence ID" value="XM_018854576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEM5"/>
    <dbReference type="GeneID" id="30027552"/>
    <dbReference type="OrthoDB" id="4084534at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="739" mass="83657" checksum="4E337FE1D3557AD8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEP5</accession>
    <name>A0A1A0HEP5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Formate dehydrogenase</fullName>
            <shortName evidence="5">FDH</shortName>
            <ecNumber evidence="2 5">1.17.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NAD-dependent formate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_31336</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the NAD(+)-dependent oxidation of formate to carbon dioxide. Formate oxidation is the final step in the methanol oxidation pathway in methylotrophic microorganisms. Has a role in the detoxification of exogenous formate in non-methylotrophic organisms.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>formate + NAD(+) = CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:15985"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.9" evidence="2 5"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712941.1">
        <property type="nucleotide sequence ID" value="XM_018855831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEP5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEP5"/>
    <dbReference type="GeneID" id="30028807"/>
    <dbReference type="OrthoDB" id="418179at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042183">
        <property type="term" value="P:formate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000057">
        <property type="entry name" value="Formate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03210">
        <property type="entry name" value="Formate_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033689">
        <property type="entry name" value="FDH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005750">
        <property type="entry name" value="PRK07574.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938">
        <property type="entry name" value="FORMATE DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42938:SF9">
        <property type="entry name" value="FORMATE DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="7">
        <location>
            <begin position="40"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="130"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="176"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="244"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="325"/>
            <end position="328"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="5">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="5">
        <location>
            <position position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="41320" checksum="57AA55B68975200E" modified="2016-10-05" version="1">MPKPTVLLVLYEGAHHARQEPKLLGCIENELGIRDFVELNGYDLVCTTSKDPIGGSEVDRLLASAEIVITTPFYPAYISRERIEQAPNLRLCITAGVGSDHVDLAAANEHKLTVAEVTGSNVQSVAEHVMMTILDLVRNFVPAHEQAMQGGWDIAAVAKDTYDLEGKVVATVGAGRIGYRVLERLVAFNPKKLLYFDYQDLPRAAVDKLNAASELFNGHGDIVQRVEKLEDMLAQADVVTINCPLHEGSKGLFNKELISHMKDGAWLVNTARGAICVEQDVADAVSLGKLRGYGGDVWYPQPAPDSHPWRSFRNKYGGGNAMTPHVSGTSIDAQARYAAGTRDILESYFSKKFDYRAQDLIVIDGKYATRAYGAENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HES4</accession>
    <name>A0A1A0HES4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein S8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_87048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712997.1">
        <property type="nucleotide sequence ID" value="XM_018859273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HES4"/>
    <dbReference type="STRING" id="869754.A0A1A0HES4"/>
    <dbReference type="GeneID" id="30032248"/>
    <dbReference type="OrthoDB" id="409928at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000006">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="156" mass="17529" checksum="FBD580D18351CFCA" modified="2016-10-05" version="1">MSLVHLSNFCAHIKNCTNVRLSTTSVPFSRLHLQVALGLYKEGFLSSIQKGSTSGPDQLPVDVTPDNISTRRLWLGLKYRKNMPVIRDMSMVLKPGRKVNMTTTEVKALASGLPVRFIKPLQPAEVMFLRTPNKEIFEIQEAAKKDMEGMVLCRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEX0</accession>
    <name>A0A1A0HEX0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_229405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713042.1">
        <property type="nucleotide sequence ID" value="XM_018855225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEX0"/>
    <dbReference type="GeneID" id="30028201"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="152" mass="15834" checksum="D5C22028A2C911C9" modified="2016-10-05" version="1">MRRAAELSLLPVPAACFACSRWQTILNKITGKTERNECSGTPVTPVLYCGVCWAVVSSEEFGGGIDEMENSAGTGDTDDTGDTGSPDETHTDSTDITDCNEKHGITDSPGNPGMTDQSDSTGNPGMTDQSDSTGNPGMTDQSDSTGNTDITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEX2</accession>
    <name>A0A1A0HEX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_153691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712954.1">
        <property type="nucleotide sequence ID" value="XM_018854593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEX2"/>
    <dbReference type="GeneID" id="30027569"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291816" evidence="2">
        <location>
            <begin position="18"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="194" mass="21455" checksum="7995194150E7840A" modified="2016-10-05" version="1">MQLRWAIMALAAGSVTAHGSHPSESSSILSTTSTFLTHDNPEDHSEDHPEDYYNTWYYVSILGTSFFPGQHDQHDQHDQHHHESDGNHHSEYHSLWKSPGSPINPSSTSDIPQIQNSRSRNFRGLSTMSSTSQRDMETTGCVSASHTLAKSDSVFATTAFSSSTDSRNGGDFNWAKLNVKAYRAVFIVGLSILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF23</accession>
    <name>A0A1A0HF23_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_161611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713076.1">
        <property type="nucleotide sequence ID" value="XM_018854626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF23"/>
    <dbReference type="GeneID" id="30027602"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="20435" checksum="8D516E0511247CC3" modified="2016-10-05" version="1">MFVALYRDFLSWKCSHCSSATHLPGVYHICMELPNLTALFKAHESVGAAISCMPLCLSVPQYSFTAKQVVKDKCEMKDMLVSRAVLYSPKGLALEHPIMIWQILLIISYFMLFEATPFYLGAQKNITCFFNFGVFPKSHTLLANLKGIEMGRHLYSNSVMQNPLVQASQGLCRHSLHNIKGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFJ8</accession>
    <name>A0A1A0HFJ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase expression protein 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_38187</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for translation of the mitochondrial OLI1 transcript coding for the mitochondrial ATP synthase subunit 9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Binds to the 5'UTR of the OLI1 mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AEP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713257.1">
        <property type="nucleotide sequence ID" value="XM_018856967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFJ8"/>
    <dbReference type="GeneID" id="30029943"/>
    <dbReference type="OrthoDB" id="4077974at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024319">
        <property type="entry name" value="ATPase_expression_mit"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12921">
        <property type="entry name" value="ATP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="23"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="644" mass="71508" checksum="36437B076B7204B9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFV9</accession>
    <name>A0A1A0HFV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate dehydrogenase assembly factor 2, mitochondrial</fullName>
            <shortName evidence="4">SDH assembly factor 2</shortName>
            <shortName evidence="4">SDHAF2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37193</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit of the SDH catalytic dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with the flavoprotein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SDHAF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713263.1">
        <property type="nucleotide sequence ID" value="XM_018856838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFV9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFV9"/>
    <dbReference type="GeneID" id="30029814"/>
    <dbReference type="OrthoDB" id="284292at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.250:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase assembly factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.250">
        <property type="entry name" value="Flavinator of succinate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03057">
        <property type="entry name" value="SDHAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005631">
        <property type="entry name" value="SDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036714">
        <property type="entry name" value="SDH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028882">
        <property type="entry name" value="SDHAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469">
        <property type="entry name" value="PROTEIN EMI5 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12469:SF2">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03937">
        <property type="entry name" value="Sdh5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109910">
        <property type="entry name" value="YgfY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="139" mass="16552" checksum="469BEFE0DC07DF85" modified="2016-10-05" version="1">MFSKSVRQTRFVRCLNHGKPQNPSYNELAARIDPIVRTDELIDVKRARLIYQSRKRGILESDLLLSRFAKKHLLSFSMEQLNEYDQLLDEPDWDIYYWATKNYDVTPLPQKWRDSEILALLQKDTENNDGEILRMPDLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG80</accession>
    <name>A0A1A0HG80_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_35728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713492.1">
        <property type="nucleotide sequence ID" value="XM_018856648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG80"/>
    <dbReference type="STRING" id="869754.A0A1A0HG80"/>
    <dbReference type="GeneID" id="30029624"/>
    <dbReference type="OrthoDB" id="4082726at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11957">
        <property type="entry name" value="efThoc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="478" mass="52018" checksum="211E67CB253FF66A" modified="2016-10-05" version="1" fragment="single">MALAGDGGGCAGAKPAFYAVWQQACDELQRAAPPAVLGVFWAYVEARAQRMRATVFRPDSTPDRIAVLGLCNAVTDRPYVRRGGGGGGPLDSYAKDTFRDALHARVRVFAASMLRFDDVTGLNKAFRAARRVPREPPLRATGSADDHLLEDAMRLRGLLRDPGALLQDPRRLAEHAAALAKLHAYLLGEETKYRAIHPVRDGRAVPAAGHAAGPAAAARQHADPAFFPEHYWLSVFAAEQSGARFDELCAEDQRRAQARFDSSKFRRLLLLQIYLASCFFVEIAAGRKAHTLARAGAPAAARHPVEAATPPELVPRFAKLRRDTVRVVRAWDPAWAATLEGLARAEGLWWAWLLSGAPAGAAPVDGAALRATEQRLRAAAPEKTRRYFHTHATPQLSRRMREPTGLLLLRGARRGGRDYAAELARVAARIDAEHDAAARAALVEERTVLLWQQTRALRGAAWLSLDGHLEAAALDAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGK1</accession>
    <name>A0A1A0HGK1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713488.1">
        <property type="nucleotide sequence ID" value="XM_018855433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGK1"/>
    <dbReference type="GeneID" id="30028409"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6411" checksum="0E338CBAA022F451" modified="2016-10-05" version="1" fragment="single">ESAQAVWTSLLRLVQTHQSVARKCDLGLEVPAVCGIHLAGTISDDRVSGLCWQILPSLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGP3</accession>
    <name>A0A1A0HGP3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Mitochondrial glyco protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_96647</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713504.1">
        <property type="nucleotide sequence ID" value="XM_018859432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGP3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGP3"/>
    <dbReference type="GeneID" id="30032407"/>
    <dbReference type="OrthoDB" id="278212at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.280.10">
        <property type="entry name" value="Mitochondrial glycoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003428">
        <property type="entry name" value="MAM33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036561">
        <property type="entry name" value="MAM33_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826">
        <property type="entry name" value="COMPLEMENT COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10826:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT 1 Q SUBCOMPONENT-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02330">
        <property type="entry name" value="MAM33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54529">
        <property type="entry name" value="Mitochondrial glycoprotein MAM33-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="256" mass="29246" checksum="DFFE04F17F08DB63" modified="2016-10-05" version="1">MSRFISRVAFSGVCRSSLPQLARRAVLPISTRSFSSAVVRHQKLADIVKSEYKVAMAVDNELEPEQEKFLEKNGFSVIESEYDSNVELRKTLPSGEEIRVFFDINEVSDVELRAPEFEGEEGAEAEQFEEELDQFDNTFANVKVLVSKPESNEGIFFNLLLQNSEEDLMVDYFNYKPDVTKFLTQIKEQGTFLAATEYQGPQFSNLDESLQLGMEKYLENKGINEELADFIFAFAEVKEETAYRKLLSNVSKFLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS0</accession>
    <name>A0A1A0HGS0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713558.1">
        <property type="nucleotide sequence ID" value="XM_018855448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS0"/>
    <dbReference type="GeneID" id="30028424"/>
    <dbReference type="OrthoDB" id="10649636at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="51715" checksum="BF918D5F89354176" modified="2016-10-05" version="1">MNEPGTSTTPVLTSAPEIGTLYGSEVQAAFEMNYTSNIMIAVSMTEPAKDIETMVESVINYSLSLPKPIEKEIPTEITEAKRYILKELEESERFKVLLAASSMSLMNKYFPKITEYRDRFRNGNRKIAHAMLKNLPYLVTENGFSWQKKRDAGHVLAALIESANLNEKNIRNDAFDELIVILTLIRNWTGENVRVIKEIVKLAGKYDEKYEKNDINHPPARTLLPLLLTGYPTAGTIDIDQWYHGEANYKKCKVSYEKMVTDFADNVWPKMHSQAKKREAGVLNTKQRILLQTTIESIGCTEADFRSEQLGIAHQGGIHAIQTAGNKQEKTRVNKYATNPEKVLSILMRILNEQPEICPNCLRIHKLEDCNALADSPRENMAAYLMPNYSFTAKNLIPKSTVYTTTFIRRRRGYMTPSPQTMNASDNILTLSPNRSATVSTMRFRLTKTWSGDTPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHB5</accession>
    <name>A0A1A0HHB5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_103253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713748.1">
        <property type="nucleotide sequence ID" value="XM_018853738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHB5"/>
    <dbReference type="GeneID" id="30026714"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="161" mass="17911" checksum="CA5102F8877578E9" modified="2016-10-05" version="1">MCCWARRMYDAILYFRTNKINEPGPATISITALLPRVVFTGCETARVPEPAETTTSWTSSVQIILTPAPVQPSVIVFEKQVHSDRAHSRKCFLLCSHSGNLRRKNPILGHVADKTLNWLGNALQFSLYTCCNHYPGALSRASTRVLGHQKKSRKACLVLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHC9</accession>
    <name>A0A1A0HHC9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">cystathionine gamma-synthase</fullName>
            <ecNumber evidence="10">2.5.1.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">O-succinylhomoserine (thiol)-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_35553</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-succinyl-L-homoserine + L-cysteine = L,L-cystathionine + succinate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20397"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="EC" id="2.5.1.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-cystathionine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the trans-sulfuration enzymes family. MET7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.48" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713886.1">
        <property type="nucleotide sequence ID" value="XM_018856623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHC9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHC9"/>
    <dbReference type="GeneID" id="30029599"/>
    <dbReference type="OrthoDB" id="10047078at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003962">
        <property type="term" value="F:cystathionine gamma-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000111">
        <property type="entry name" value="Cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000063">
        <property type="entry name" value="Probable cystathionine gamma-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051750">
        <property type="entry name" value="Trans-sulfuration_enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42699:SF1">
        <property type="entry name" value="CYSTATHIONINE GAMMA-SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="170"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="592" mass="66619" checksum="1BF104B3E8AE4A21" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHD3</accession>
    <name>A0A1A0HHD3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_105094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713892.1">
        <property type="nucleotide sequence ID" value="XM_018853786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHD3"/>
    <dbReference type="GeneID" id="30026762"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="57" mass="6725" checksum="1C3FDB14B082CD62" modified="2016-10-05" version="1">MSQIVKFTFLINFYFGSCPDVEFDLISNPNTRAHDSTRFFHFAFDLEKSIQIKSMNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHG4</accession>
    <name>A0A1A0HHG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_30042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714085.1">
        <property type="nucleotide sequence ID" value="XM_018855540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHG4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHG4"/>
    <dbReference type="GeneID" id="30028516"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="2">
        <location>
            <begin position="1058"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="972"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1240" mass="139961" checksum="CF2E9477C7F05943" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHK1</accession>
    <name>A0A1A0HHK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF3445 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29852</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713838.1">
        <property type="nucleotide sequence ID" value="XM_018855496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHK1"/>
    <dbReference type="GeneID" id="30028472"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="44652" checksum="21E6CA60DE9D5A1F" modified="2016-10-05" version="1">MLQFSFLVENVLLATAILILTIVFAKGLRYSLKPKTNLENSKANLFTVAPVSLDFKWDEEAPLKSYPFKDKEYKLTMGIRNIDISEWLLIENTYLKRIEEKTKITTNSHPAYPASRDLKKSTVFSSPEAEAAVREFYDIAITYMCEKYPMYFEHVDDQIKNRITNELIPAKSSCVKDHKDLLPYLIRTIEEDFIILLKDSSKKDQPYGEEYYFKAGVFAFAAGFDPADKFNQPLTMIHKPIPGYESKLKLSMNRFFDKIKTGALVSRSNFSIQTHAKFFVDDENKGYHLTEEELNTPIPFENLNFEKQVHYRSERQVLTKLPESEAIVFTIRTYLHPMSDFAENMEAANRLRGTLDKFPDDMARYKNITQPRPAVMRYIDELLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHM8</accession>
    <name>A0A1A0HHM8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Presequence translocated-associated motor subunit PAM17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_29957</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the PAM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Mitochondrion inner membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the PAM17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713991.1">
        <property type="nucleotide sequence ID" value="XM_018855522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHM8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHM8"/>
    <dbReference type="GeneID" id="30028498"/>
    <dbReference type="OrthoDB" id="5970083at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013875">
        <property type="entry name" value="Pam17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28021:SF1">
        <property type="entry name" value="PRESEQUENCE TRANSLOCATED-ASSOCIATED MOTOR SUBUNIT PAM17, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08566">
        <property type="entry name" value="Pam17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="6 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16972" checksum="D4813AA90E4FE488" modified="2016-10-05" version="1">MNWVDFFKLRKESKRISIVASSLTSVAGAFATLTYLGNVEIDVEKPIMGIDPFMVMGGVVIIGGVAGYLAGPSIGVRVFNMKNSKKLPEFMVKEQNFLQRVQRNRVDPSSQSFSNPVPDYYGERIYSLDNYKQWLRDCNAFRRKTQEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI07</accession>
    <name>A0A1A0HI07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Delta(14)-sterol reductase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Sterol C14-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_37375</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714117.1">
        <property type="nucleotide sequence ID" value="XM_018856866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI07"/>
    <dbReference type="STRING" id="869754.A0A1A0HI07"/>
    <dbReference type="GeneID" id="30029842"/>
    <dbReference type="OrthoDB" id="10262235at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 13" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="441" mass="50489" checksum="B9D4470AFA891A61" modified="2016-10-05" version="1">MSKLNPTTTHREFSGVPGALAIATGLPVILVLFYYLCNETYSVQGISVSVEQVRAQLPQSLHEFAQLCFDKQCWAAYTAWFSILAVLDIYAPGKDIPGVTLRDGTRLMYRINGTSMSGLLFALLLARLLSLENYFMPELQFLYDNQLKLTLTTIIASFAMSFFVYAMSFIPLRRPNGADTKERILAVPGNTGNPIYDWFIGRELNPRIGAWDIKLFCELRPGMLLLFLINLSCAHNQYHTTGKVTDAMMLVNFLEAFYIFDGVLNEEGCLTMIDIATDGFGFMLAFGDLAWLPWSYSLQCRYLALPQNYYELGPTRTVLILVTSFVGYYIFLSSNQQKSDFKNGKLDHMDSISTPTGSKLLVDGWWKLSQHINYFGDWIIGWSWCLPTGSMTVFTYFYVIYFATLLIHRQTRDEAKCRDKYGKAWEEYERRVPYKIVPYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI81</accession>
    <name>A0A1A0HI81_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_76577</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714073.1">
        <property type="nucleotide sequence ID" value="XM_018858555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI81"/>
    <dbReference type="STRING" id="869754.A0A1A0HI81"/>
    <dbReference type="GeneID" id="30031531"/>
    <dbReference type="OrthoDB" id="6339427at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015798">
        <property type="term" value="P:myo-inositol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015791">
        <property type="term" value="P:polyol transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050814">
        <property type="entry name" value="Myo-inositol_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020:SF9">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48020">
        <property type="entry name" value="PROTON MYO-INOSITOL COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="487"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="84"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="548" mass="61245" checksum="4A13F77493B7E536" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIB7</accession>
    <name>A0A1A0HIB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_30018</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714064.1">
        <property type="nucleotide sequence ID" value="XM_018855534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIB7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIB7"/>
    <dbReference type="GeneID" id="30028510"/>
    <dbReference type="OrthoDB" id="329563at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007091">
        <property type="term" value="P:metaphase/anaphase transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="171"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="487"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="521"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="555"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="657"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="252"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="703" mass="80459" checksum="E6CB164F2B8F6BCB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HID9</accession>
    <name>A0A1A0HID9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_115009</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714415.1">
        <property type="nucleotide sequence ID" value="XM_018854007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HID9"/>
    <dbReference type="STRING" id="869754.A0A1A0HID9"/>
    <dbReference type="GeneID" id="30026983"/>
    <dbReference type="OrthoDB" id="185618at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015007">
        <property type="entry name" value="NUP2/50/61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF141">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08911">
        <property type="entry name" value="NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RanBD1" evidence="11">
        <location>
            <begin position="328"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="446" mass="48829" checksum="E687C98AD17BFD38" modified="2016-10-05" version="1">MAKRSADEQITREGLGLSHNSNEDVCDERASADIISKRKILQPKGRNFQFSRRTTESSTKNTNSTTPHTEKAEKLKALGNNFSKAVLSVKLDQCVPDYRALAKKYLMYYEEIEIDAQSTGKVNASKDISGSKDEVAEMRKGNDVVNPFAGVSTSNKKSSDVKEISELGNISDESELQSDGEKIDVKIHGPKFSLSAIPTTKSTTFSFGPKKSTKKANSDSDSEPEVEVKGPSFTFNKEIKDPVFKLPANQAISSGFVAQRVTISKPNEAIHSKFEALPVAQSSGFTSLNVSHGSELPNNDVFSQKPIQDEKASTEENINSNNEIKPILENDDDGESVLFEVKAKLMLLRKDNAKDPYQSLGVGELKLLKSLKRSTLRVLLRAEGGLRLLLNALLLKDVQYSTLGNGSFVRLPALDDQHQIVTYVLKVGSAEKASKLCDALNAAKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIE7</accession>
    <name>A0A1A0HIE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase DBP9</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent RNA helicase dbp9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_30092</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding RNA helicase involved in the biogenesis of 60S ribosomal subunits and is required for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714138.1">
        <property type="nucleotide sequence ID" value="XM_018855555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIE7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIE7"/>
    <dbReference type="GeneID" id="30028531"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033677">
        <property type="term" value="F:DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="46"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="238"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="331"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="15">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA23657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="592" mass="67156" checksum="977109717D56D6FB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIL5</accession>
    <name>A0A1A0HIL5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_10061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018853677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIL5"/>
    <dbReference type="GeneID" id="30026653"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="255" mass="27967" checksum="64678F40E3DACAD9" modified="2016-10-05" version="1">MTWPVRLVWRRLSLPGPWPQLVFEGPAKFRFNGQYSVQRGGARARSQKMDSSPPMGCFVGPSSQSAVAPAWVQNYVMASPPLCSSELGNTAIRPRRPQLGSGLGRFIGAVPPGVSAGNSDISFHCRSEYYSCVATQEAPAKATQRPAHMPKKPGQYTHETPTTQDRLHRQRKATRHAAPLLWGRFPPRYKAPVWTLLALVGALLRGHTRLRRPVYGAARHTQTHNKTAPNSRGRLAEKAQEKGRSPIGARPAAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIQ9</accession>
    <name>A0A1A0HIQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zf-CHY-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_112675</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714253.1">
        <property type="nucleotide sequence ID" value="XM_018853950.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HIQ9"/>
    <dbReference type="GeneID" id="30026926"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16464">
        <property type="entry name" value="RING-H2_Pirh2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039512">
        <property type="entry name" value="RCHY1_zinc-ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14599">
        <property type="entry name" value="zinc_ribbon_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="7">
        <location>
            <begin position="516"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="8">
        <location>
            <begin position="587"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="654"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="128"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="459"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="151"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="887" mass="95482" checksum="AACF8D4AFAA334E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIR9</accession>
    <name>A0A1A0HIR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U3 small nucleolar ribonucleoprotein protein IMP3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">U3 small nucleolar ribonucleoprotein protein imp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_38363</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714263.1">
        <property type="nucleotide sequence ID" value="XM_018856990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIR9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIR9"/>
    <dbReference type="GeneID" id="30029966"/>
    <dbReference type="OrthoDB" id="10248812at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140691">
        <property type="term" value="F:RNA folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000006">
        <property type="entry name" value="U3 small nucleolar ribonucleoprotein IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="183" mass="21658" checksum="C02A209A1938D6BA" modified="2016-10-05" version="1">MVRQLKHHEQKLLKKVDFLNWKQDQGHRDTQVMSTYHIQNREDYHKYNKICGEIRQLAHKLSLLQPTDPFRIKHEQLLLEKLYNMGILATKSKISDLENKISVSAFCRRRIGVVMCRMRMAQNIKDANTFVEQGHVRVGPNVITDSAYLVTRNLEDYLTWVDSSKIKRNVLKYKNKIDDYDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIS6</accession>
    <name>A0A1A0HIS6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_35262</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714387.1">
        <property type="nucleotide sequence ID" value="XM_018856588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIS6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIS6"/>
    <dbReference type="GeneID" id="30029564"/>
    <dbReference type="OrthoDB" id="3437016at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF191">
        <property type="entry name" value="VACUOLAR BASIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="371"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="540"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="66"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="570" mass="61410" checksum="46630DD3031D4FBD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIU7</accession>
    <name>A0A1A0HIU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_76850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714416.1">
        <property type="nucleotide sequence ID" value="XM_018858598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIU7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIU7"/>
    <dbReference type="GeneID" id="30031574"/>
    <dbReference type="OrthoDB" id="10263513at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="104" mass="11394" checksum="046764704637E151" modified="2016-10-05" version="1">MAPKIQQTKAAKAAAALAGGKKGKKKWNKGKVKDKAQHVVILDQDKYDRIMKEVPGFKFVSVSVLVDRLKIGGSLARIALRQLESDGVIKPVVKHSKQAIYTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ72</accession>
    <name>A0A1A0HJ72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">tRNA pseudouridine synthase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">tRNA pseudouridylate synthase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">tRNA-uridine isomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_34192</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr). Catalyzes pseudouridylation at position 44 in U2 snRNA. Also catalyzes pseudouridylation of mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a uridine in tRNA = a pseudouridine in tRNA</text>
            <dbReference type="Rhea" id="RHEA:54572"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13934"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714685.1">
        <property type="nucleotide sequence ID" value="XM_018856449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ72"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ72"/>
    <dbReference type="GeneID" id="30029425"/>
    <dbReference type="OrthoDB" id="10256309at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="17">
        <location>
            <begin position="267"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="15">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00053072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00073968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA24204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="471" mass="53135" checksum="566F8DF1AA2BF0F3" modified="2016-10-05" version="1">MVLTSSEWKPLVDADGNPVEKSERKPKKKVACMIGYCGTGYNGMQIQNNPDVKTIEAELFRALIAAGAVSKENSTDLKKNGFMRAARTDKGVHAAGNVVSCKLILEDDTLAKVNAALPSQIRMWGIERVNRSFDCRKMCSSRIYEYLLPTHSLLPPRPKSSLFNLIEASRAEHPGVLRDDADQAWWQATRAAILASGVAQHELEQVFEKTAETVFDQDKAELRKEYYDAEGEVSEWGRLVRSVRRVENSCRRGFRISDAKLQLFRDAMKQYEGSHNFHNFTLGKTYGDPSAQRFMKGTSVLAPFIIEGSEWVSIKIHGQLFMLHQIRKMVCMASLVVRTGCPITRISDCFGPAKINIPKAPALGLLLECPVYETYNERLAEMGYNHLEFSGYETEMNAFKMKNIYDKIYAEETKENVYHGFFEFLDTFKGSGATKEGRHIFDFLGAVFNNQMENKETKKSSAPPKAPDNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJC4</accession>
    <name>A0A1A0HJC4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prohibitin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_77092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Prohibitin probably acts as a holdase/unfoldase for the stabilization of newly synthesized mitochondrial proteins. Involved in mitophagy; may act as an adapter for ATG8 that supports mitophagosome assembly. Negatively regulates the proteolytic processing of ATG32 via the i-AAA protease. Acts as a negative regulator of the m-AAA protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">The mitochondrial prohibitin complex consists of two subunits (PHB1 and PHB2). The subunits assemble into a membrane-associated ring-shaped supercomplex of approximately 1 mDa. The mitochondrial prohibitin complex interacts with the m-AAA protease, a heterohexamer composed of YTA12/RCA1 and YTA10/AFG3. The mitochondrial prohibitin complex interacts with ATG8 and the interaction may support mitophagosome assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the prohibitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714740.1">
        <property type="nucleotide sequence ID" value="XM_018858637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJC4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJC4"/>
    <dbReference type="GeneID" id="30031613"/>
    <dbReference type="OrthoDB" id="275637at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035632">
        <property type="term" value="C:mitochondrial prohibitin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140580">
        <property type="term" value="F:mitochondrion autophagosome adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03401">
        <property type="entry name" value="SPFH_prohibitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000001">
        <property type="entry name" value="Prohibitin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000163">
        <property type="entry name" value="Prohibitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222">
        <property type="entry name" value="PROHIBITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23222:SF1">
        <property type="entry name" value="PROHIBITIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00679">
        <property type="entry name" value="PROHIBITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Band 7" evidence="9">
        <location>
            <begin position="54"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="302" mass="33750" checksum="A1749AE33A431CF4" modified="2016-10-05" version="1">MNDPQWQKVTQELRRRMAQNGTRGGSPPKSPLGMFAGLGGLLLLGGASMLALNALFNVDGGQRAIIYSRLHGIQNQIYPEGTHFVIPWFQRPIVFDVRAKPRNVSSLTGTKDLQMVNITCRVLFKPDLYQLPNIYRTLGQDYDEKVLPSIVNEVLKSVIAQFNASQLITQREKVSRLVKENLVRRASKFNIQLDDVSLTFMTFSPEFSAAVEAKQIAQQDAQRAAFVVDKAIQEKQQLVVRASGEAKSAELIGDAIKKSKDYVELKRLDTAREIALILATSPNRILLDNDTLLLNTVVDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD8</accession>
    <name>A0A1A0HJD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_77103</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Has a physiological role leading to 18S rRNA stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714755.1">
        <property type="nucleotide sequence ID" value="XM_018858640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJD8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJD8"/>
    <dbReference type="GeneID" id="30031616"/>
    <dbReference type="OrthoDB" id="278325at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000461">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1230.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1230.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001931">
        <property type="entry name" value="Ribosomal_eS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018279">
        <property type="entry name" value="Ribosomal_eS21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038579">
        <property type="entry name" value="Ribosomal_eS21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10442">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01249">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002148">
        <property type="entry name" value="Ribosomal_S21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00996">
        <property type="entry name" value="RIBOSOMAL_S21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="87" mass="9638" checksum="D1D3DFE28C3ACA2A" modified="2016-10-05" version="1">MENDKGQLVELYVPRKCSASNRIIKAKDHASVQINIANVDAEGRAIPGDNTTYALSGYIRSRGEADDSLNRLAQQDGLLKNVWSYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJJ0</accession>
    <name>A0A1A0HJJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">CCT-beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_52436</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714810.1">
        <property type="nucleotide sequence ID" value="XM_018858289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJJ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJJ0"/>
    <dbReference type="GeneID" id="30031265"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="526" mass="56727" checksum="8B109CBD991CED13" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJQ4</accession>
    <name>A0A1A0HJQ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PLP-dependent transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_37726</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714885.1">
        <property type="nucleotide sequence ID" value="XM_018856907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJQ4"/>
    <dbReference type="GeneID" id="30029883"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000066">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="43"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="47361" checksum="D88EE50FD4DB59F0" modified="2016-10-05" version="1">MLRPSTRSFSVAARLAKWKEVPLAPPDKILGISEAYQKDSNSKKINLGVGAYRDNSGKPIVFPSVKKAETVLLEKETDKEYTGIIGSKNYQNAVRNFIFNNSDKDANGAKLIGDNRIVTAQTISGTGSLRVIADFLKRFYSVEKIIVPKPTWANHVAVFTDAGISADYYAYYDKSNNSLDYANLKQSISAAENESVILLHACCHNPTGMDLTAEQWEEVLAIIQEKKLFPLIDMAYQGFASGSPYKDISLIRRMNELVVEGKIPTYALCQSFAKNMGLYGERTGSISIVTESAAQSSAIESQLKKLIRPMYSNPPIHGSRIVETIFSDQNLLNEWLADLDSVVGRLNVVRDKLYQKLDKTNYNWDHLLKQRGMFIYTGLSAKQVIELREKYSVYATEDGRFSISGVNENNVDYLADAINKVISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJT0</accession>
    <name>A0A1A0HJT0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA (uracil(54)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77104</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>uridine(54) in tRNA + S-adenosyl-L-methionine = 5-methyluridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42712"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714756.1">
        <property type="nucleotide sequence ID" value="XM_018858641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJT0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJT0"/>
    <dbReference type="GeneID" id="30031617"/>
    <dbReference type="OrthoDB" id="10250660at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051908">
        <property type="term" value="F:double-stranded DNA 5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030697">
        <property type="term" value="F:tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000201">
        <property type="entry name" value="TRM2p tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000174">
        <property type="entry name" value="TRM2p tRNA methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030390">
        <property type="entry name" value="MeTrfase_TrmA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025795">
        <property type="entry name" value="tRNA_(uracil-5-)_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="RNA M5U METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061:SF30">
        <property type="entry name" value="TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51622">
        <property type="entry name" value="SAM_MT_RNA_M5U_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01230">
        <property type="entry name" value="TRMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 9 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="64"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="530" mass="59941" checksum="26205B105AA52F94" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKE8</accession>
    <name>A0A1A0HKE8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_81581</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PGA52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714759.1">
        <property type="nucleotide sequence ID" value="XM_018859213.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HKE8"/>
    <dbReference type="GeneID" id="30032189"/>
    <dbReference type="OrthoDB" id="118256at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018805">
        <property type="entry name" value="YJL171C/Tos1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018807">
        <property type="entry name" value="YJL171C/Tos1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31737:SF2">
        <property type="entry name" value="PROTEIN TOS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10287">
        <property type="entry name" value="YJL171C_Tos1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10290">
        <property type="entry name" value="YJL171C_Tos1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 N-terminal" evidence="11">
        <location>
            <begin position="87"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="Cell wall protein YJL171C/Tos1 C-terminal" evidence="10">
        <location>
            <begin position="237"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="9">
        <location>
            <begin position="1024"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="199"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="600"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="648"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="854"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="207"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="605"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="668"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="689"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="861"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="889"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1299" mass="138348" checksum="D71A56FE4022DB30" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKH6</accession>
    <name>A0A1A0HKH6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Carbamoyl phosphate synthase arginine-specific large chain</fullName>
            <ecNumber evidence="14">6.3.4.16</ecNumber>
            <ecNumber evidence="5">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Ammonium-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Arginine-specific carbamoyl phosphate synthetase, ammonia chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Carbamoyl phosphate synthase arginine-specific large chain, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Glutamine-dependent carbamoyl phosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">METBIDRAFT_30681</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20">Large subunit of the arginine-specific carbamoyl phosphate synthase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, hydrogencarbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer composed of 2 chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="14"/>
    <dbReference type="EC" id="6.3.5.5" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714878.1">
        <property type="nucleotide sequence ID" value="XM_018855681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKH6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKH6"/>
    <dbReference type="GeneID" id="30028657"/>
    <dbReference type="OrthoDB" id="1924069at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000004">
        <property type="entry name" value="Carbamoyl-phosphate synthase (glutamine-hydrolyzing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000015">
        <property type="entry name" value="Carbamoyl-phosphate synthase arginine-specific large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1030.10:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000002">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006275">
        <property type="entry name" value="CPSase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01369">
        <property type="entry name" value="CPSaseII_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009455">
        <property type="entry name" value="PRK12815.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="24">
        <location>
            <begin position="182"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="24">
        <location>
            <begin position="718"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="25">
        <location>
            <begin position="986"/>
            <end position="1142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00068891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00074189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="OBA24397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1142" mass="125172" checksum="09C3B010999BD841" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKJ1</accession>
    <name>A0A1A0HKJ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715026.1">
        <property type="nucleotide sequence ID" value="XM_018855707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKJ1"/>
    <dbReference type="GeneID" id="30028683"/>
    <dbReference type="OrthoDB" id="4072912at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="245" mass="27753" checksum="8B9C4B0F4180D209" modified="2016-10-05" version="1">MFDVSPVSSNLGASQNPKPDFRQDQSSGSIAPSVESLRKKNTSLRVLHPNTPLPGRWWGSQLQEKNPSGSSRIPKEKKNPFAIKNASLTHLDDESSMDEFFETVNELSLLQQADRFSEYSPLSSALEDFDPEIRPHMTEPLLPDGSPLNFSAIKLCRGETNIYSATPLARVWEFNSDTDKHEYSILNILADEFENLDEDASEPKQSLWSQFRKLTVGLNEDEYNECYPWAKYCIKSLLDGEEDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKW7</accession>
    <name>A0A1A0HKW7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715018.1">
        <property type="nucleotide sequence ID" value="XM_018855706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKW7"/>
    <dbReference type="STRING" id="869754.A0A1A0HKW7"/>
    <dbReference type="GeneID" id="30028682"/>
    <dbReference type="OrthoDB" id="3996692at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="506"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="534" mass="60675" checksum="EA7FE9CDA250C08A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ24</accession>
    <name>A0A1A0GZ24_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_48120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709277.1">
        <property type="nucleotide sequence ID" value="XM_018858264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ24"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ24"/>
    <dbReference type="GeneID" id="30031240"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="16"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA16978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="37580" checksum="D798812CEF03E85D" modified="2016-10-05" version="1">MKLSYSEAAFKISICLGIASSFIVRSNTVEITTGEKLLEDLLVKRVNYSIVNSPRVHFVGHVYILGSLLVSSTNNLEASVRINSDEFTNYGTVAFNTIQSDFPSTYYVNTHDSFINTGSMFFGISGATSGTIPFRVTSVKSWNNTGMMIFWTASGESAQVLLAQDVGHNDSSIIKNSGSICLYNTMWQATTSIAENGCITIGTGSAVILNLALNSHCFSISKMQTFYLEGPDSVLTISGLNSSCTFPMIKVAGFGNENVIEFDIWHHDVSSYEYLTTRGELIVKVVKESKVVFHIGTGYLEQSFRLRLSTTGCKISYSPHAPNIPPYECSCQSVFPEVSGATCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H2B0</accession>
    <name>A0A1A0H2B0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AMPKBI-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_47653</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 5'-AMP-activated protein kinase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709457.1">
        <property type="nucleotide sequence ID" value="XM_018858203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H2B0"/>
    <dbReference type="STRING" id="869754.A0A1A0H2B0"/>
    <dbReference type="GeneID" id="30031179"/>
    <dbReference type="OrthoDB" id="531008at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000562">
        <property type="entry name" value="Snf1 kinase complex beta-subunit Gal83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006828">
        <property type="entry name" value="ASC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037256">
        <property type="entry name" value="ASC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF84">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04739">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01010">
        <property type="entry name" value="AMPKBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160219">
        <property type="entry name" value="AMPKBI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Association with the SNF1 complex (ASC)" evidence="5">
        <location>
            <begin position="244"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="19"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA18062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="359" mass="39834" checksum="229715649BFE2E86" modified="2016-10-05" version="1">MSDQNDISMLDVSNALDSIGAEAAPPAPARRKSTLILTDDGEPENTNTGRATDPAQSGAMAASAAEPTAPQTQPARPELPGPAPDASATVPVDISWLQGGDKVYVTGSFTGWRKMIGLQSQPDGTHVVTLGLPVGTHRFRFVIDNELRFSDFLPTATDQMGNFVNYVEVTPEHVQMHMRQQRSLEEQQQQKQAREQAAQQLRQGLRQRALAGRPRSDLIFKFISDDDDMGNGYLRYYDDDADAAADRRYNYISDIPPIFVEPKVMEQYYMAVDEQAKAQNGQQAWLHPPQLPPQLDHVILNNFNSMDRHNNSGALPIPNHVVLNHLATTSIKHNTLAVASVVRYKHKYVTQVLYAPLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V2</accession>
    <name>A0A1A0H4V2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_223386</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709472.1">
        <property type="nucleotide sequence ID" value="XM_018855159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V2"/>
    <dbReference type="GeneID" id="30028135"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA18937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="21101" checksum="FDE7B9F6A0EC66D0" modified="2016-10-05" version="1">MANVTFRPASISPPAKNSRLSIGRDYEGKWLHLFIGIPGFPIEKACFCRRWSSWDTANRSTGACWSWCALAVFHFRYFEDTVIWRSGWWPQFGHLSTAARGQLWTLTREISISKYLCLRSKQAVFWNTTLGMNRAFAPHPSKNRSSKFLCSLFCSKSATVCFWWAVAFTLQARAYRQLHDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Z6</accession>
    <name>A0A1A0H4Z6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LicD/FKTN/FKRP nucleotidyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709517.1">
        <property type="nucleotide sequence ID" value="XM_018858146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Z6"/>
    <dbReference type="GeneID" id="30031122"/>
    <dbReference type="OrthoDB" id="444255at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009644">
        <property type="entry name" value="FKTN/MNN4/W02B3.4-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007074">
        <property type="entry name" value="LicD/FKTN/FKRP_NTP_transf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407">
        <property type="entry name" value="FUKUTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15407:SF28">
        <property type="entry name" value="RIBITOL-5-PHOSPHATE TRANSFERASE FKTN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04991">
        <property type="entry name" value="LicD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="LicD/FKTN/FKRP nucleotidyltransferase" evidence="6">
        <location>
            <begin position="396"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="727"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA18982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="749" mass="87083" checksum="DFA943163DD77EAF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5E8</accession>
    <name>A0A1A0H5E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arginase/deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_46562</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the arginase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709664.1">
        <property type="nucleotide sequence ID" value="XM_018858053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5E8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5E8"/>
    <dbReference type="GeneID" id="30031029"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF28">
        <property type="entry name" value="HYPOTHETICAL ARGINASE FAMILY PROTEIN (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="385" mass="42065" checksum="D295C5591BC1F1F6" modified="2016-10-05" version="1">MQRLSEIKSSHEQSARNSAHSGYLRLRAQVNLPHDDDTDDLSHGKTMFGGIVTFGRFDTFDCYDPALYDSPIDIAIVGAPFDTGTSYRPGARFGPEAIRLGGRRLKEGVTPVRTNGPGSKLGRVDPYNPKAHNLSIIDCGDVPMTPFDNRIALNQLYRGQRAIHNHTTSGKPLIPHTKILTMGGDHTITLMALRSAYEKYGRLAVLHFDSHIDTWDPKKLGGGITDYMSLNHGTFLHYAAENGYLEPHKCFHVGLRAPYIDPSYDQKHDEECGFNTITARDIDKMGTAGILSKLKETLGDIPVYISVDIDVLDPAYAPGTGTMEVGGWTTRELLTVIDGLEGLNVVGVDVVEVSPPFDTNSEVTSLAATAVMDSILGLLIVQTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5S6</accession>
    <name>A0A1A0H5S6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">JmjC domain-containing histone demethylation protein 1</fullName>
            <ecNumber evidence="5">1.14.11.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">[Histone-H3]-lysine-36 demethylase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_13568</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N(6),N(6)-dimethyl-L-lysyl(36)-[histone H3] + 2 2-oxoglutarate + 2 O2 = L-lysyl(36)-[histone H3] + 2 formaldehyde + 2 succinate + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:42032"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:9787"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="1.14.11.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the JHDM1 histone demethylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.27" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709800.1">
        <property type="nucleotide sequence ID" value="XM_018854391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5S6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5S6"/>
    <dbReference type="GeneID" id="30027367"/>
    <dbReference type="OrthoDB" id="5876800at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140680">
        <property type="term" value="F:histone H3K36me/H3K36me2 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15517">
        <property type="entry name" value="PHD_TCF19_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041070">
        <property type="entry name" value="JHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050690">
        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17811">
        <property type="entry name" value="JHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="20">
        <location>
            <begin position="54"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="21">
        <location>
            <begin position="273"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA19268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="616" mass="70914" checksum="B493474A38847D4B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5U7</accession>
    <name>A0A1A0H5U7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Protein transport protein SEC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_79817</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709820.1">
        <property type="nucleotide sequence ID" value="XM_018859131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5U7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5U7"/>
    <dbReference type="GeneID" id="30032107"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990753">
        <property type="term" value="C:equatorial cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01479">
        <property type="entry name" value="Sec24-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041742">
        <property type="entry name" value="Sec24-like_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF39">
        <property type="entry name" value="SECRETORY 24AB, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="14">
        <location>
            <begin position="254"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="15">
        <location>
            <begin position="328"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="17">
        <location>
            <begin position="575"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="16">
        <location>
            <begin position="668"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="13">
        <location>
            <begin position="814"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA19288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="20">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="958" mass="105844" checksum="4846AD50D78B19BC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Y8</accession>
    <name>A0A1A0H5Y8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ANTH-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_47140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in endocytosis and clathrin cage assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with PAN1 and the clathrin heavy and light chains CHC1 and CLC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AP180 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709902.1">
        <property type="nucleotide sequence ID" value="XM_018858130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Y8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Y8"/>
    <dbReference type="GeneID" id="30031106"/>
    <dbReference type="OrthoDB" id="44015at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005545">
        <property type="term" value="F:1-phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16988">
        <property type="entry name" value="ANTH_N_YAP180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.150:FF:000004">
        <property type="entry name" value="ENTH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.150">
        <property type="entry name" value="ANTH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011417">
        <property type="entry name" value="ANTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014712">
        <property type="entry name" value="ANTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045192">
        <property type="entry name" value="AP180-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951">
        <property type="entry name" value="CLATHRIN ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22951:SF5">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-BINDING CLATHRIN ASSEMBLY PROTEIN LAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07651">
        <property type="entry name" value="ANTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00273">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENTH" evidence="8">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="293"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="447"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="410"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="456"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00058079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="637" mass="71604" checksum="AEEDD829BE8C6D95" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H600</accession>
    <name>A0A1A0H600_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_221883</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709875.1">
        <property type="nucleotide sequence ID" value="XM_018855147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H600"/>
    <dbReference type="STRING" id="869754.A0A1A0H600"/>
    <dbReference type="GeneID" id="30028123"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="361"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="434"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="500"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="527"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="611" mass="66400" checksum="F0CC77D8BB683125" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6H7</accession>
    <name>A0A1A0H6H7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_33285</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRP1/MDG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710164.1">
        <property type="nucleotide sequence ID" value="XM_018856260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6H7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6H7"/>
    <dbReference type="GeneID" id="30029236"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032640">
        <property type="entry name" value="AMPK1_CBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050827">
        <property type="entry name" value="CRP1_MDG1_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343">
        <property type="entry name" value="5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10343:SF81">
        <property type="entry name" value="CRUCIFORM DNA-RECOGNIZING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16561">
        <property type="entry name" value="AMPK1_CBM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-activated protein kinase glycogen-binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="235"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="468"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="192"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="497"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="511" mass="53665" checksum="638C77B7777F3126" modified="2016-10-05" version="1">MSSTYTFKWPSGPKDVIVTGTFDEWSKSLPLVKQADGSFELTVPFADDVRVLYKYVVDGEWLTNSAQSIDTDSNGIENNVLTADVLTAANPNSQIPEAGGLEVKSGLLSTTVMPSTEGEQTTLGEPGIVIPTDKDQLAAFTTFENTERDLNTKAAEAPEAAAVLTPEEKKKQKKKLKRTKYKLNKKAKVAQSTETSSSTESSPVPEEEVAGIAAAAAVVEETVIVPAEAITTADEPAAEAADVVQKSVSGESETAVEEKEPAPYSLAEKDITAPAVVPVEAESDTQAPAEADKEPAPYALAEDDIAQPTVVPADEKAAEEKKEEETRETLDPGVTRAVGTGAVAGALAGVVASEISQQKAVEPTIQPAIGEPIESPKELAAPVAAAPDAASSENGSAAPASTKEVTALPVNGAKHESEEEIIIAQGGHDLKAIEKQIAAGEQGPVSVEQLQPTVSEAKQLVEEAHIPLEEVQAEPVPAKQKNSASSGGKVPKTDKEKKKRGFFSKLKKMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U5</accession>
    <name>A0A1A0H6U5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_33281</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710160.1">
        <property type="nucleotide sequence ID" value="XM_018856259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U5"/>
    <dbReference type="GeneID" id="30029235"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF20">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291876" description="glucan endo-1,3-beta-D-glucosidase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="11">
        <location>
            <begin position="376"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="12">
        <location>
            <begin position="712"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="64"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="267"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="269"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="281"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="301"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1087" mass="121489" checksum="390669FCBFBB32A0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H791</accession>
    <name>A0A1A0H791_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">GPI ethanolamine phosphate transferase 1</fullName>
            <ecNumber evidence="14">2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_79189</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the PIGG/PIGN/PIGO family. PIGN subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-" evidence="14"/>
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        <property type="protein sequence ID" value="OBA19845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710370.1">
        <property type="nucleotide sequence ID" value="XM_018859016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H791"/>
    <dbReference type="STRING" id="869754.A0A1A0H791"/>
    <dbReference type="GeneID" id="30031992"/>
    <dbReference type="OrthoDB" id="2748310at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015867">
        <property type="term" value="P:ATP transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16020">
        <property type="entry name" value="GPI_EPT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000015">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007070">
        <property type="entry name" value="GPI_EtnP_transferase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017852">
        <property type="entry name" value="GPI_EtnP_transferase_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037671">
        <property type="entry name" value="PIGN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250:SF0">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12250">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04987">
        <property type="entry name" value="PigN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 14" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="528"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="672"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="705"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="737"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="795"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="841"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="874"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="907"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="GPI ethanolamine phosphate transferase 1 C-terminal" evidence="15">
        <location>
            <begin position="457"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA19845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="969" mass="110251" checksum="17513AD534874CDC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7N8</accession>
    <name>A0A1A0H7N8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein AFG2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_79240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710432.1">
        <property type="nucleotide sequence ID" value="XM_018859023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7N8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7N8"/>
    <dbReference type="GeneID" id="30031999"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19503">
        <property type="entry name" value="RecA-like_CDC48_NLV2_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001721">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="4">
        <location>
            <begin position="26"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="256"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="542"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="773" mass="83704" checksum="7D5D581F79528E7F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H835</accession>
    <name>A0A1A0H835_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710703.1">
        <property type="nucleotide sequence ID" value="XM_018857609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H835"/>
    <dbReference type="GeneID" id="30030585"/>
    <dbReference type="OrthoDB" id="4081634at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="126" mass="14551" checksum="0CE802BC9D8EDE1D" modified="2016-10-05" version="1">MPKKGRSVATDPSSFTHRDLLLVTQLLHTLGLITLEQVQASDRLDDLAEDWYVHKSTLLSRRQGQFPLENPPTGQQLRKLYENMLEDNEPCATTTDLANKFYFIRVGELESRISEYKTEFHSLLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H838</accession>
    <name>A0A1A0H838_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ClpS-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79362</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710582.1">
        <property type="nucleotide sequence ID" value="XM_018859047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H838"/>
    <dbReference type="STRING" id="869754.A0A1A0H838"/>
    <dbReference type="GeneID" id="30032023"/>
    <dbReference type="OrthoDB" id="250175at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L7/L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.710">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00368">
        <property type="entry name" value="Ribosomal_bL12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000206">
        <property type="entry name" value="Ribosomal_bL12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013823">
        <property type="entry name" value="Ribosomal_bL12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_bL12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008932">
        <property type="entry name" value="Ribosomal_bL12_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036235">
        <property type="entry name" value="Ribosomal_bL12_oligo_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45987:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL12M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00542">
        <property type="entry name" value="Ribosomal_L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16320">
        <property type="entry name" value="Ribosomal_L12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="ClpS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48300">
        <property type="entry name" value="Ribosomal protein L7/12, oligomerisation (N-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL12 oligomerization" evidence="5">
        <location>
            <begin position="39"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein bL12 C-terminal" evidence="4">
        <location>
            <begin position="101"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="168" mass="17970" checksum="10E1998E2D85DD42" modified="2016-10-05" version="1">MLRQIARPASRAFARTSTRFSVRFNSAAAEATSAPVDPKISQIVDQISTLTLLETSSLVSELKTRLNISDIALPSAGTAAPAPAAPVEEEVKEVVEEQTIFSIKLESFDAKAKPKIIKEVKGLLGLSLVESKKFVEAAPKVLKENVAKEDADKIKATLEGLGAKVVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H872</accession>
    <name>A0A1A0H872_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_44945</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710618.1">
        <property type="nucleotide sequence ID" value="XM_018857853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H872"/>
    <dbReference type="STRING" id="869754.A0A1A0H872"/>
    <dbReference type="GeneID" id="30030829"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="338"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="380"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA20093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="443" mass="51220" checksum="1D40F89E4FE0611C" modified="2016-10-05" version="1">MEYYSSLDASKLDQETPTLFELISANQLEALLSPSLRYVLVHYAQRYPGYLLKITNKFDEINLLLRTLVEWYFFKYWRGSFTENFYGIKRVSQTPLSSKKYNASKLTQLTPSMIEERRDLSRVQSLVSIFEVTGLSYLREKLNYQYELLYSKLITNQLNATEATSTGEARYIKLQLLFVRFYPFVQFACKFSNLITTLLYLSGSCKSPSLLTFLFKVNYARLNQYDYSRNEPSLSSDKSSKLDRVNPPSNLERFANILKDYVAKPSLKLSKIILGTIFPIAIFSLKFLEWWNNSNFAQTVAKSQRNDLEEPIPPPSLLKALFIYEQNPAKFFRANSSCPLCKDEIHNPAIIETGHVFCYTCIFNYLRDSHKLVRMKKAASENGLSSEEEPEQEKDDTQEKRIYSKSEPGTSELFFIDKGGRCPITGQKLLGCKWNEVTGEFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8A5</accession>
    <name>A0A1A0H8A5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Dipeptidyl aminopeptidase B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_78699</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710778.1">
        <property type="nucleotide sequence ID" value="XM_018858922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8A5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8A5"/>
    <dbReference type="GeneID" id="30031898"/>
    <dbReference type="OrthoDB" id="16520at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF200">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 10, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="15">
        <location>
            <begin position="148"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="14">
        <location>
            <begin position="620"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="838" mass="94626" checksum="4E2B47A6AE923140" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C7</accession>
    <name>A0A1A0H8C7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Homoserine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_79430</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710668.1">
        <property type="nucleotide sequence ID" value="XM_018859065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C7"/>
    <dbReference type="STRING" id="869754.A0A1A0H8C7"/>
    <dbReference type="GeneID" id="30032041"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009092">
        <property type="term" value="P:homoserine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001209">
        <property type="entry name" value="PRK00175.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF16">
        <property type="entry name" value="SERINE O-SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="122"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="500" mass="55896" checksum="1F4D4562B7D64FFB" modified="2016-10-05" version="1">MFLRARKDLSPERFSSCLMELHGSNPQLAFPCLDRLEQKTKELADAAGTSESSSVSVASSESCITEVSSLESGPEPAYSRVSSGFGLYRNKNPTFFDNGGYLPEYQIAYESWGTLSPNKDNLVLIHTGLSASSHAKSQPKNTKNGWWENFIGSGKYIDTDKYFVVCTNVLGGCYGSTGPSSKDPANNDYYATRFPIVTVNDMVRAQRELVRDVFGVKKLHASVGASMGGMQSLAYAWEFPDEVENVISISGCARSHPYSIALRHTQRQVLMSDPNWNRGFYYEKVPPHIGMKLAREIATVTYRSGPEWESRFGRSRADDSKPVAFCPDFLVETYLDHAGEKFCLEYDANSLLYVSKAMDLFDLGLANRARAQASRVQAERAFKEKQDDESAGKVDKTNVPDEPYQEKNTANISEEESLKDLEDGLKKISHKNILVVGVESDILFPVWQQREIASLLRKTSPHKNNIEYFEMGTDVSNYGHDTFLLSLDDFGPHVKNFLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8K0</accession>
    <name>A0A1A0H8K0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_78666</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710734.1">
        <property type="nucleotide sequence ID" value="XM_018858912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8K0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8K0"/>
    <dbReference type="GeneID" id="30031888"/>
    <dbReference type="OrthoDB" id="365981at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020796">
        <property type="entry name" value="ORC5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047088">
        <property type="entry name" value="ORC5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048866">
        <property type="entry name" value="ORC5_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12705:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14630">
        <property type="entry name" value="ORC5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21639">
        <property type="entry name" value="ORC5_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="8">
        <location>
            <begin position="10"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="ORC5 lid" evidence="10">
        <location>
            <begin position="219"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 5 C-terminal" evidence="9">
        <location>
            <begin position="303"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="479" mass="55047" checksum="F6B15D4BBC9DCAD7" modified="2016-10-05" version="1">MEQPAKYVKFRENEQDLLAAFITRNAEALAANVIVLGYKSVGKTHVVLRHLENLGIRRTVLNCDEFITQKILLQNCLHKIKADSSVDLSKYTQRYMYKGLEVARLSALCETFAHFLMALDQFVQETGYSEPHVLVLDRFDQCLEATDDLFRSFVLMRENSAIRNISVVYVISNQAPHQVSTLDVPCIPFMPYSLEQVTEIVAQNPFTEVREGKVNYTFWAQFAKLVVDLHFDYTGSDLSLVWDICLNLWPLFVAYAQEEQDFLAIYRKLKPELVKDEVISNSAVTMYETGFLEDDTSAPYADLPHHSKFILIAAYLASYNDQKMDVQLFSKVKSPKKRSPRKLPEKSHDSPLKNAAIDSRLLSASYFDLERMKAILSVIYRNESHTLSKDNQEYFNLYHDLTERDLAKKETEFNTFTLNKNVDLNVQIATLAGLGLISSTYSKDPLSNKIRWRCNVGWDVIDSIAQDMKFPLLNYTVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8R5</accession>
    <name>A0A1A0H8R5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_12406</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710912.1">
        <property type="nucleotide sequence ID" value="XM_018854225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8R5"/>
    <dbReference type="GeneID" id="30027201"/>
    <dbReference type="OrthoDB" id="1750432at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18027" checksum="F4EF90F0E02F45D7" modified="2016-10-05" version="1">MSEGPVFLYTPYGDKEIETVPSDKIQPDTRIVRAPSIWLKPTGPTPDTVPLLDVLINSDYAAAALISTGSNADFISDKLADQIGTKKSRLPYECVCQLAIAFAVPITHSVELQFEANGKRFVRTCLVFPDMRIELIFGAQFVIDFPTVDVVKKTFNGKPMYNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8S2</accession>
    <name>A0A1A0H8S2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein IFH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42973</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710804.1">
        <property type="nucleotide sequence ID" value="XM_018857605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8S2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8S2"/>
    <dbReference type="GeneID" id="30030581"/>
    <dbReference type="OrthoDB" id="4047468at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018837">
        <property type="entry name" value="TF_CRF1/IFH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28057:SF1">
        <property type="entry name" value="PROTEIN IFH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10380">
        <property type="entry name" value="CRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="604"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="332"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="623"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="870" mass="97531" checksum="4BA606CF105FB234" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8V4</accession>
    <name>A0A1A0H8V4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="20">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_43309</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="20"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710952.1">
        <property type="nucleotide sequence ID" value="XM_018857644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8V4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8V4"/>
    <dbReference type="GeneID" id="30030620"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990530">
        <property type="term" value="C:Cdc50p-Drs2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090555">
        <property type="term" value="F:phosphatidylethanolamine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="ATPase_P-type"/>
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    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
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        <property type="entry name" value="CATATPASE"/>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
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    <proteinExistence type="inferred from homology"/>
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            <end position="334"/>
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        <location>
            <begin position="362"/>
            <end position="383"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="883"/>
            <end position="903"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="923"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="966"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1007"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1040"/>
            <end position="1059"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="1071"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="21">
        <location>
            <begin position="47"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="22">
        <location>
            <begin position="852"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="17">
        <location>
            <position position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
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        <location>
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        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
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        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>ATP</name>
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        <location>
            <position position="565"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
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        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="623"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="703"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        <location>
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            <name>ATP</name>
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        <location>
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        <location>
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            <name>ATP</name>
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        <ligand>
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        <ligand>
            <name>ATP</name>
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        <ligand>
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        <ligand>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00004166"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00023034"/>
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    </evidence>
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    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00034036"/>
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            <dbReference type="ARBA" id="ARBA00049128"/>
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            <dbReference type="ARBA" id="ARBA00051303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
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    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA20430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1181" mass="132278" checksum="C5FDEBB9774758AF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8W2</accession>
    <name>A0A1A0H8W2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">UPF0057-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78742</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710844.1">
        <property type="nucleotide sequence ID" value="XM_018858931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8W2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8W2"/>
    <dbReference type="GeneID" id="30031907"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01309">
        <property type="entry name" value="UPF0057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="55" mass="5939" checksum="D09533FEC3233815" modified="2016-10-05" version="1">MVCGDILKIIIAILLPPLGVFLEVGCSGAFFLNILLTLLGYIPGIIHALYIILTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H919</accession>
    <name>A0A1A0H919_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-5-taurinomethyluridine 2-sulfurtransferase</fullName>
            <ecNumber evidence="3">2.8.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_44277</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of mitochondrial tRNA(Lys), tRNA(Glu) and tRNA(Gln). Required for the formation of 5-taurinomethyl-2-thiouridine (tm5s2U) of mitochondrial tRNA(Lys), tRNA(Glu), and tRNA(Gln) at the wobble position. ATP is required to activate the C2 atom of the wobble base.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-taurinomethyluridine(34) in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 5-taurinomethyl-2-thiouridine(34) in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11732"/>
            <dbReference type="Rhea" id="RHEA-COMP:11733"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:87171"/>
            <dbReference type="ChEBI" id="CHEBI:87172"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.14" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710900.1">
        <property type="nucleotide sequence ID" value="XM_018857770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H919"/>
    <dbReference type="STRING" id="869754.A0A1A0H919"/>
    <dbReference type="GeneID" id="30030746"/>
    <dbReference type="OrthoDB" id="3685at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103016">
        <property type="term" value="F:tRNA-uridine 2-sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990799">
        <property type="term" value="P:mitochondrial tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="MnmA_TRMU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.280:FF:000001">
        <property type="entry name" value="tRNA-specific 2-thiouridylase MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="MnmA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046885">
        <property type="entry name" value="MnmA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046884">
        <property type="entry name" value="MnmA-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="MnmA-like_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001138">
        <property type="entry name" value="PRK00143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933:SF5">
        <property type="entry name" value="MITOCHONDRIAL TRNA-SPECIFIC 2-THIOURIDYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11933">
        <property type="entry name" value="TRNA 5-METHYLAMINOMETHYL-2-THIOURIDYLATE -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03054">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20258">
        <property type="entry name" value="tRNA_Me_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20259">
        <property type="entry name" value="tRNA_Me_trans_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like central" evidence="13">
        <location>
            <begin position="200"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-specific 2-thiouridylase MnmA-like C-terminal" evidence="12">
        <location>
            <begin position="278"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF20259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="360" mass="39799" checksum="30B276E9785E087F" modified="2016-10-05" version="1">MSGGVDSSVTAALYASQYANVRGIYMANWSQTAKCTEADWVDVKRVCAQLGVPCERVNFEKDYWNDVFSPMVDMYQQGLTPNPDLGCNRHIKFGRMAAHLGLGEPRAGAKRWLATGHYARVVPEASGVCSLAQASEPTKDQLYYLASVPQAVLAHVLMPLGHYRKSDVRRLARDRFHLHTAAKPDSVGLCFVEPGHTKFRDFLNEYIAPNPGNIVTEDGRVWGRHQGLWHATIGQKLGISMPQGDPAYKGVWFVSEKRVHSNELVIVKGRDHPALYKQGVRVCDFEWFGLRPATLPMDTLCMRYQSLHEPVAVQALEEGLGGTATIVLHRGQRAMAPGQNVVLYAGQKVVASGRITDTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H954</accession>
    <name>A0A1A0H954_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine(9)-N1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA methyltransferase 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1G9)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_43974</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(9) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43156"/>
            <dbReference type="Rhea" id="RHEA-COMP:10367"/>
            <dbReference type="Rhea" id="RHEA-COMP:10368"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.221"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.221" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711063.1">
        <property type="nucleotide sequence ID" value="XM_018857727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H954"/>
    <dbReference type="STRING" id="869754.A0A1A0H954"/>
    <dbReference type="GeneID" id="30030703"/>
    <dbReference type="OrthoDB" id="278300at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052905">
        <property type="term" value="F:tRNA (guanosine(9)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18089">
        <property type="entry name" value="SPOUT_Trm10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028564">
        <property type="entry name" value="MT_TRM10-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038459">
        <property type="entry name" value="MT_TRM10-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016653">
        <property type="entry name" value="TRM10/TRM10A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007356">
        <property type="entry name" value="tRNA_m1G_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563">
        <property type="entry name" value="TRNA (GUANINE-9-) METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13563:SF13">
        <property type="entry name" value="TRNA METHYLTRANSFERASE 10 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016323">
        <property type="entry name" value="tRNA_m1G_mtfrase_met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51675">
        <property type="entry name" value="SAM_MT_TRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase TRM10-type" evidence="12">
        <location>
            <begin position="88"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="29"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="281"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="297"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR016323-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="36217" checksum="3939D1040510A616" modified="2016-10-05" version="1">MSSTNSPPNETANLEHAIVGEFKRQKVELPEGMSKNAWKRLQKQQRWEEQKDDFRLKRREKKRVARQRKSERKMKGLDDEESNYHQEAKKELPQKQEPSGVNIIMDCEFDELMNDKEIVSMSNQITRCYSAKRHSKYEVGLTISSFNKRLRQRFDKSIQNYAKWKNIQFEENETLEDLLPADEKERAKYVYLTADTDEELLQLEEGHTYILGGIVDKNRHKELCFNKAKKLGLRVGRLPIGKYIQMNSRHVLATSHVYEIMCMWLENKDWEKSFNSVLPPRKLKKDNSEGVSDELVTKTQEANTDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H961</accession>
    <name>A0A1A0H961_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab proteins geranylgeranyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710944.1">
        <property type="nucleotide sequence ID" value="XM_018858945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H961"/>
    <dbReference type="STRING" id="869754.A0A1A0H961"/>
    <dbReference type="GeneID" id="30031921"/>
    <dbReference type="OrthoDB" id="1923006at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017230">
        <property type="entry name" value="Mrs6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF4">
        <property type="entry name" value="CHM, RAB ESCORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037514">
        <property type="entry name" value="Rab_ger_ger_transf_A_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00894">
        <property type="entry name" value="YEASTMRS6P"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR037514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="609" mass="66563" checksum="0DF24F360D4141F3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H975</accession>
    <name>A0A1A0H975_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710950.1">
        <property type="nucleotide sequence ID" value="XM_018856048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H975"/>
    <dbReference type="GeneID" id="30029024"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16568" checksum="3C87E14ECFBE1D9B" modified="2016-10-05" version="1">MSKDFDVVEYLLFTCYQKVICPPVGWGYLIVGNITSLVVVVLSGKKDQIKQRKQKHNQNQNKNKNNKTKTQQSQHKNKTKSTKTSECVGSLESGYAQWKISRCDAAYPDANHNTMGSATCEPIIFGYSSKSLQKASHGLTQCSGICCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H980</accession>
    <name>A0A1A0H980_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative family 17 glucosidase SCW4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78918</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711098.1">
        <property type="nucleotide sequence ID" value="XM_018858966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H980"/>
    <dbReference type="STRING" id="869754.A0A1A0H980"/>
    <dbReference type="GeneID" id="30031942"/>
    <dbReference type="OrthoDB" id="941679at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050732">
        <property type="entry name" value="Beta-glucan_modifiers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631:SF14">
        <property type="entry name" value="FAMILY 17 GLUCOSIDASE SCW10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16631">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291694" evidence="5">
        <location>
            <begin position="18"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="36647" checksum="13A97F08412E90BA" modified="2016-10-05" version="1">MLFNTLVKVALVATAFAQPVQHQHHQHEKKDVVTHTVIVTVNGDAADSAETLIPEVPDVVTSLIASSVTPSAASATPASSTSSTSVSSSSSSFSADVKGITYSPYTDAGGCKTKEQIAAEFEALTGYEIIRLYGVDCSQVEYVLAAMSSSQKLFAGIYDMTSISDDVATLAAAVEAANGWDQVYTVSIGNELVNSGEATASQVKEYVAEGRTALTNAGYTGPVVSVDTFIAIYNNIDLCDISDYVAANAHAYFDGGYAAADAGTWVYNQYTGLQKFCSGKEVFIVESGWPTAGDTNGDAIAGTSEQAAALSDIADKVGDHVLYFNAYNDGWKDPGYLSCEQYWGIFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9B4</accession>
    <name>A0A1A0H9B4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Exo-1,3-beta-glucanase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_78997</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711231.1">
        <property type="nucleotide sequence ID" value="XM_018858981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9B4"/>
    <dbReference type="STRING" id="869754.A0A1A0H9B4"/>
    <dbReference type="GeneID" id="30031957"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 12 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="13">
        <location>
            <begin position="59"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="389" mass="44433" checksum="A3B46DC32B055162" modified="2016-10-05" version="1">MRLAYSLLSLTTVLGVNLGGWFVLEPYMTPSFFEPYNDEIVDEYHLTQKLGQKDAQKLLEKHWKSWYTEKDFREIAAAGLNTVRIPIGYWAFVLLQDDPYVTGQQKYLDKAIDWARKHNLKIWIDLHGAPGSQNGFDNSGLRDQIEYQTDGNIKATLTALQSIFNKYGDEKYLDVISGIEMLNEPFGPLSDMNQLKNFYGWANANARSVTKNTLVFHDAFQSFNYWDDFFTTDAYSNVIVDHHHYQVFSQLELEMTIDEHILVACGWGTSALTESVTNVCGEFSAALTDCAPWLNGVGRGARWSGDYDSSSLSNLCTAYTHLSDWTAEHTENVRKYIEAQLDAFELTGGWIFWNWKTEDAIDWDMARLIAAGVFPQPLSDRKYPNQCGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9H2</accession>
    <name>A0A1A0H9H2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711162.1">
        <property type="nucleotide sequence ID" value="XM_018857722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9H2"/>
    <dbReference type="STRING" id="869754.A0A1A0H9H2"/>
    <dbReference type="GeneID" id="30030698"/>
    <dbReference type="OrthoDB" id="203724at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024420">
        <property type="entry name" value="TRAPP_III_complex_Trs85"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975:SF6">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12975">
        <property type="entry name" value="TRANSPORT PROTEIN TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12739">
        <property type="entry name" value="TRAPPC-Trs85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="636" mass="70233" checksum="9C65DD350E24C64F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9K8</accession>
    <name>A0A1A0H9K8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_43993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711223.1">
        <property type="nucleotide sequence ID" value="XM_018857732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9K8"/>
    <dbReference type="GeneID" id="30030708"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291697" evidence="1">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="66" mass="7119" checksum="0ECF35D9AA32E7D4" modified="2016-10-05" version="1" fragment="single">KIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPKVATGAKTIPMLMKYIVVPGTSRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9L5</accession>
    <name>A0A1A0H9L5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-binding protein RAP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_42965</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the regulation of telomere length, clustering and has a specific role in telomere position effect (TPE).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Chromosome</location>
            <location evidence="8">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the RAP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711099.1">
        <property type="nucleotide sequence ID" value="XM_018857603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9L5"/>
    <dbReference type="STRING" id="869754.A0A1A0H9L5"/>
    <dbReference type="GeneID" id="30030579"/>
    <dbReference type="OrthoDB" id="435460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070187">
        <property type="term" value="C:shelterin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031848">
        <property type="term" value="P:protection from non-homologous end joining at telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11655">
        <property type="entry name" value="rap1_myb-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021661">
        <property type="entry name" value="Rap1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015280">
        <property type="entry name" value="Rap1_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039595">
        <property type="entry name" value="TE2IP/Rap1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466">
        <property type="entry name" value="TELOMERE REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16466:SF6">
        <property type="entry name" value="TELOMERIC REPEAT-BINDING FACTOR 2-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09197">
        <property type="entry name" value="Rap1-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11626">
        <property type="entry name" value="Rap1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2 8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0779">Telomere</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Rap1 DNA-binding" evidence="10">
        <location>
            <begin position="228"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="TRF2-interacting telomeric protein/Rap1 C-terminal" evidence="11">
        <location>
            <begin position="469"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="303"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="303"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF09197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF11626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="560" mass="64694" checksum="36FC132CF3CB54CC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q6</accession>
    <name>A0A1A0H9Q6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Biosynthesis of nicotinic acid protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BNA5</name>
        <name evidence="6" type="ORF">METBIDRAFT_79012</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>L-kynurenine + H2O = anthranilate + L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711247.1">
        <property type="nucleotide sequence ID" value="XM_018858985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Q6"/>
    <dbReference type="GeneID" id="30031961"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000031">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084:SF0">
        <property type="entry name" value="KYNURENINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22580">
        <property type="entry name" value="KYNU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="51529" checksum="07398FC288904D39" modified="2016-10-05" version="1">MAQTNHHPDTSKDAALDTQFGCHRHEFVVPTFLSLGVSSPAFGAADPAIYFCGNSLGLMPKSTRPAIEAELQAWGERAVEAHFNHPDGNKTMWMDIDLPLVDLLAPIVGAKPTEVAAMGSLTANLNALLVSFYKPGGRRTKILMEKNAFPSDYYALLNMVKMHGHDESHLIQLDVPAGETYLQTQRVLDAIEQHQDELALVLLPGIQYYTGQFFDIGTITQYAQQRGVPVGWDLAHAVGNVVLRLHEWNVDFAAWCSYKYLNSGPGAIGGIYVHERYTQNNCSDSFAPRLAGWWGNDAAQRFQMLEHFDPIQSALSYRQLNPSVIDCVALQSSLEIFKKAGGMGELRKKSEALTGHLEALLVRSRYCLSAGAPGAAFGFRILTPASPAERGCQLSLLFQPHADDPAQNVMEQVHRYLHEHAIICDERRPDVLRLAPVPLYNTFREVSIVVARLEEALGQIEKRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9T1</accession>
    <name>A0A1A0H9T1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_78960</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase g subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711155.1">
        <property type="nucleotide sequence ID" value="XM_018858977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9T1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9T1"/>
    <dbReference type="GeneID" id="30031953"/>
    <dbReference type="OrthoDB" id="437at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006808">
        <property type="entry name" value="ATP_synth_F0_gsu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04718">
        <property type="entry name" value="ATP-synt_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="125" mass="13438" checksum="3BDF0771212954FC" modified="2016-10-05" version="1">MSAIINKATGLVNTAVAKSTQLANCAVYWGKVGAEIGKTVYKAEGLAPPSLAQFKQAYEASLQFLKTPAQQKALQQKVMSLPMNKDTLTKAAVYGIHGLAFFSVGEIIGRRNFFGYPALGHAEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA64</accession>
    <name>A0A1A0HA64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_171217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711529.1">
        <property type="nucleotide sequence ID" value="XM_018854694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA64"/>
    <dbReference type="GeneID" id="30027670"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="166" mass="18866" checksum="3D86639E0DE666B8" modified="2016-10-05" version="1">MPRGPPEKQNNQVCHNHAHNDVTTVFTILSTIMTLPCLQPCPQLSHNHIPKLVYKYVYHRKYVYQKLACKNTLSARQCKSFDWVFSPHGGKTNIHNRNQKPERLFVQKIGISAVPGKTPFSKAQLCQIHRLIVLQNTSWAGHLKVGHTTHMTGLCSHAVVQHQTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAF0</accession>
    <name>A0A1A0HAF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_170239</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711490.1">
        <property type="nucleotide sequence ID" value="XM_018854682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAF0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAF0"/>
    <dbReference type="GeneID" id="30027658"/>
    <dbReference type="OrthoDB" id="366230at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045503">
        <property type="term" value="F:dynein light chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010970">
        <property type="term" value="P:transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050687">
        <property type="entry name" value="Dynein_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442:SF22">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 INTERMEDIATE CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12442">
        <property type="entry name" value="DYNEIN INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="444"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="597" mass="65785" checksum="3725ECCBC78FB64D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAH2</accession>
    <name>A0A1A0HAH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_175701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711639.1">
        <property type="nucleotide sequence ID" value="XM_018854722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAH2"/>
    <dbReference type="GeneID" id="30027698"/>
    <dbReference type="OrthoDB" id="4019224at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="390" mass="41703" checksum="67EA21E108F91B31" modified="2016-10-05" version="1">MSTQNTTPTPSPNPLKPTRAATPSTPPAAKTRKRLLVEFSTPDPSQDVPFSPGLKRSSAGLLRSLRRRLNDAASPYSAASLLKTPGNPDSDSDGRDTPRQAKLMKTPQYFSPGRRLFAEDHSPNKKELGEISSQLKSRLSLAFGSLQQKEKAGSGISPVKLDFANGPYASSHDSPTRMADAFLPPARPAAAALNRTNINLQTLQASPRIGTADLASQRSHVPGDDTQPRHPVSMPSPDEESSAHNALMVALSRAKERRKSQNSEFRRPSFGLGLADQRRALHHMPHAPGPLPPKLPPISVISPPRSDGASEQEAVYSLMSLSSPQANKQDLHALQAAAQMQAHSRAEGSEGLSRSSSVAVSAPPSLAVRNYDDDETDVEDGDGSDSDMTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAL6</accession>
    <name>A0A1A0HAL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_41831</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the BRE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711555.1">
        <property type="nucleotide sequence ID" value="XM_018857456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAL6"/>
    <dbReference type="GeneID" id="30030432"/>
    <dbReference type="OrthoDB" id="654191at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16499">
        <property type="entry name" value="RING-HC_Bre1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000414">
        <property type="entry name" value="E3 ubiquitin protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08647">
        <property type="entry name" value="BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 15 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="627"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="184"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="327"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="522"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA21045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="679" mass="77380" checksum="81B8075253EA19F1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAP4</accession>
    <name>A0A1A0HAP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar sorting protein Vps3844 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_42229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711462.1">
        <property type="nucleotide sequence ID" value="XM_018857515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAP4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAP4"/>
    <dbReference type="GeneID" id="30030491"/>
    <dbReference type="OrthoDB" id="5583277at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053065">
        <property type="entry name" value="Archenteron_Induction-Rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024382">
        <property type="entry name" value="Vps3844_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853:SF1">
        <property type="entry name" value="DUF3844 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36853">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12955">
        <property type="entry name" value="Vps3844_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291720" description="Vacuolar sorting protein Vps3844 C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar sorting protein Vps3844 C-terminal" evidence="2">
        <location>
            <begin position="312"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="414" mass="44331" checksum="7FE2EFF6FB0C2670" modified="2016-10-05" version="1">MKIASFSSAVLLAASAVSAASNIAVYEFPGKHNAEVPRVSLKSSLLYLADKFGAGEYYSIGHKDKAVEFLDLVHGKEAGKPDLLVILKGVDTPERILDDWAPTFEIETGTNKKVAPGLVKVLFKKLPKQVAGAQSALRVSHLTEELKYVSSGFAGGDDMPQQFKHFNLELPQAWAKLVRHSADEAENTQHVMGALSSGLKLVNDKLYINEIMQMVKLGSSENHTDAFAVANLDSLLSIGHRIGYNSRTYNVAKGALLQSIVALDSKFDITLVAVGPDHKIASDHLQLLKRASELDGVFSTFSKRGTSGANSCFANEAECDASTKSCSGHGSCTNVQEGCWQCLCSATQDKKTSKTTKWAGYDCSKKDIAAQAHLLLWTSVVLLATLVGGVKLLFGVGSDALPGVLQAATIDTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB39</accession>
    <name>A0A1A0HB39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aminopeptidase P N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41923</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711715.1">
        <property type="nucleotide sequence ID" value="XM_018857466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB39"/>
    <dbReference type="STRING" id="869754.A0A1A0HB39"/>
    <dbReference type="GeneID" id="30030442"/>
    <dbReference type="OrthoDB" id="10261878at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01087">
        <property type="entry name" value="Prolidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052433">
        <property type="entry name" value="X-Pro_dipept-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43226:SF1">
        <property type="entry name" value="XAA-PRO DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase P N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="473" mass="54554" checksum="62A44C53B269FE62" modified="2016-10-05" version="1">MPFELGSISSQKYPSREHARKVYSHFKSIKINNPVGFFLSGENLDYYDYCDQTKPFRQNRYFYYLSGCEIPGSHVLYLADADELTLFLPDVNAEDIMWSGPPLLREEALEKYDVDNVQYSQNLKKELASLKQKEVAVFTMTFNKWSSPFAEFLTEDKNTLKFALDESRLIKDSFELSLMRRAASITDKCHLAVMSATPIETNETHIHAEFMYHALRQGSKYQLYDPICCAGPNCSTLHYVKNDDEINSEKRSILIDAGAEWSCYASDVTRCFPINGDWTKEHLEIYNVVLKMQQVTMSMIKPGALWDDIHLEAHRVLIREFIRLGIFTNADEKTIFDSDISARFFPHGLGHLLGMDTHDVGGNPRYDDSNQKLKYLRLRRELQKNMVLTDEPGVYFSPFLLDDILSDPTKLKFIDTKILDKYWYVGGVRIEDDLIITEDGFENMTKITSDPTEISEIIKSSSEKGAQRFHNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB92</accession>
    <name>A0A1A0HB92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_42404</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711909.1">
        <property type="nucleotide sequence ID" value="XM_018857539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB92"/>
    <dbReference type="STRING" id="869754.A0A1A0HB92"/>
    <dbReference type="GeneID" id="30030515"/>
    <dbReference type="OrthoDB" id="207120at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46026:SF1">
        <property type="entry name" value="RHO-TYPE GUANINE NUCLEOTIDE EXCHANGE FACTOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00499">
        <property type="entry name" value="P67PHOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="3">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="56" mass="6283" checksum="30A3355C1F04219E" modified="2016-10-05" version="1" fragment="single">MSVPFTVKALHDYKSDYDDDLQFAAGQVITVTDIEDDEWYSGTYGGQSGLFPKNFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBV2</accession>
    <name>A0A1A0HBV2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_181920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711878.1">
        <property type="nucleotide sequence ID" value="XM_018854776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBV2"/>
    <dbReference type="GeneID" id="30027752"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="101" mass="11708" checksum="A43E5559090FB23D" modified="2016-10-05" version="1">MAKLGRTGSETWDGGSGRPRNRREEEQVEKHEEEQEEKHEEKHEENHKNPKKKKNQKKGQRKAISDSISLLRHQRLRPGNGPRASHRLGAICRTGNVRDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBW9</accession>
    <name>A0A1A0HBW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711882.1">
        <property type="nucleotide sequence ID" value="XM_018857409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBW9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBW9"/>
    <dbReference type="GeneID" id="30030385"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1430.10">
        <property type="entry name" value="Yeast translation eEF2 (G' domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="148"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="989" mass="106327" checksum="33C023EC71F7A3A9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC53</accession>
    <name>A0A1A0HC53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome B pre-mRNA-processing protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31989</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711978.1">
        <property type="nucleotide sequence ID" value="XM_018855958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC53"/>
    <dbReference type="STRING" id="869754.A0A1A0HC53"/>
    <dbReference type="GeneID" id="30028934"/>
    <dbReference type="OrthoDB" id="2107880at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061671">
        <property type="term" value="C:Cbp3p-Cbp6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037653">
        <property type="entry name" value="Cbp6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28250:SF1">
        <property type="entry name" value="CYTOCHROME B PRE-MRNA-PROCESSING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20180">
        <property type="entry name" value="UQCC2_CBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13237" checksum="3EE9D45B56DD6E9E" modified="2016-10-05" version="1">MSSRAQLAEKIVSLLKTLPKDRIKHYASFKDLQLERFQKPDVVKLIPEQDLKLQYISLCDLVNDKYRNYYKLDDKLLKPKGNPQYYERILSEIKGEGKETWLSAMRTVVFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC58</accession>
    <name>A0A1A0HC58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_32084</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712101.1">
        <property type="nucleotide sequence ID" value="XM_018855982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC58"/>
    <dbReference type="STRING" id="869754.A0A1A0HC58"/>
    <dbReference type="GeneID" id="30028958"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF15">
        <property type="entry name" value="TRANSPORTER SEO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="569" mass="66010" checksum="71FCADC7E04611D1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD14</accession>
    <name>A0A1A0HD14_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_39773</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712329.1">
        <property type="nucleotide sequence ID" value="XM_018857174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD14"/>
    <dbReference type="STRING" id="869754.A0A1A0HD14"/>
    <dbReference type="GeneID" id="30030150"/>
    <dbReference type="OrthoDB" id="2123952at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050987">
        <property type="entry name" value="AtrR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910">
        <property type="entry name" value="TRANSCRIPTION FACTOR PDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46910:SF37">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="6">
        <location>
            <begin position="322"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="753" mass="84366" checksum="5012163D34010EAC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD20</accession>
    <name>A0A1A0HD20_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_139215</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712371.1">
        <property type="nucleotide sequence ID" value="XM_018854453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD20"/>
    <dbReference type="STRING" id="869754.A0A1A0HD20"/>
    <dbReference type="GeneID" id="30027429"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF678">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="398"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="3"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="468" mass="49958" checksum="7CD097EFDD80E6A8" modified="2016-10-05" version="1">MGTATVRSGSINLLNTIIGAGILAMPYGLRRNGLLFGTGLIVWSALTSAFGLYLQNKVAKYTQQQGAVSYFSLCQLTYPQLLVIFDGAIAVKCFGVGVLYLVVIGDLMPKIAESAGAPAGSVLLARNFWISVFMAAVVAPLSFLKKLDSLKYTSVVALVSVVYLICLVVEHYFADGGAADAREIDWVGPVSWRLTLTSFSIFVFAYTCHQNMFAIINELQPSEKLGLPTRQLNLIIRNAISVACLSYLVVGVLGYLTFGNAVNANIISMYPRDSVASLVGRLAIVVMVLLSFPLQCHPCRGSVNHVIHFLRHGVSSGWKLRAPVAAAEYTPIPGDTDSADVLFDPSFVSTTPMEGAQDTDGHAPIVVPLTAFRFYVITAVIVVAAYLVAISVQSLALVLAFVGATGSTLISFILPGLFSFSLIKPLDGAAVLTPLERFCKYGGLALSIWGAVVMAVCLSATLFLAPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDB3</accession>
    <name>A0A1A0HDB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">12 kDa heat shock protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712383.1">
        <property type="nucleotide sequence ID" value="XM_018857079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDB3"/>
    <dbReference type="GeneID" id="30030055"/>
    <dbReference type="OrthoDB" id="2348401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="12510" checksum="F582AA618ED775E1" modified="2016-10-05" version="1">MSDTGRKSFTDKAQEAMKPESEKSYLEKAKEGVTNAVDGFAAKNVPDSEKSFGQTVADNAKQGKDDAKDSVDQQGATIGETAQEYVAVAKEQVANAANYVSGVVTGATEGAKQVDSEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDE3</accession>
    <name>A0A1A0HDE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrofolate reductase</fullName>
            <ecNumber evidence="2">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77481</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712444.1">
        <property type="nucleotide sequence ID" value="XM_018858707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDE3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDE3"/>
    <dbReference type="GeneID" id="30031683"/>
    <dbReference type="OrthoDB" id="414698at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF3">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="8">
        <location>
            <begin position="6"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="202" mass="22916" checksum="A40E66D7F69FFE04" modified="2016-10-05" version="1">MKSTPKVSLIVAALQPEFGIGAKGKLPWRLKQEIKYFRDVTSMSREGHVNAVIMGRKTWESIPAKFRPLPGRLNVVLSRSCETSAENGVFLTNSMDSALRTLQEPDFMHENNKIDKIFVIGGAQIYNAFVADPRVDNLLITEVTYNGNPAETPVLDTFLDWDLSAWEKKTEAELLEFAGVDYTKGLVTEGDYKYEYTMWERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDL0</accession>
    <name>A0A1A0HDL0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_143617</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712478.1">
        <property type="nucleotide sequence ID" value="XM_018854501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDL0"/>
    <dbReference type="GeneID" id="30027477"/>
    <dbReference type="OrthoDB" id="3987408at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018854">
        <property type="entry name" value="Psome_chaperone_3/4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10448">
        <property type="entry name" value="POC3_POC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12397" checksum="6FD8E3AD385B7B07" modified="2016-10-05" version="1">MKTSEISVGNNDYDLAVHVTTPVSAKTPVLVYLTQRGQGKIGSYVYTIGRGTETYSSILQQGEDAGVDDLATNLGRVILKRFGCPSYVCMSGCFMPYEYGELSRQVVAACNEAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDM7</accession>
    <name>A0A1A0HDM7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pyruvate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_86753</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712576.1">
        <property type="nucleotide sequence ID" value="XM_018859265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDM7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDM7"/>
    <dbReference type="GeneID" id="30032240"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF3">
        <property type="entry name" value="TRANSAMINATED AMINO ACID DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="10">
        <location>
            <begin position="37"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="465"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="533"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="535"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="617" mass="68565" checksum="DE7A932148D33B96" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDR9</accession>
    <name>A0A1A0HDR9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_40496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712730.1">
        <property type="nucleotide sequence ID" value="XM_018857281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDR9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDR9"/>
    <dbReference type="GeneID" id="30030257"/>
    <dbReference type="OrthoDB" id="4093311at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="634"/>
            <end position="653"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="653" mass="73803" checksum="66CEEBFC126595C7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDY2</accession>
    <name>A0A1A0HDY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-8 sterol isomerase</fullName>
            <ecNumber evidence="8">5.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Delta-8--delta-7 sterol isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_38770</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reaction which results in unsaturation at C-7 in the B ring of sterols.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7 8">Steroid metabolism; ergosterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ERG2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.-.-.-" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712603.1">
        <property type="nucleotide sequence ID" value="XM_018857043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDY2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDY2"/>
    <dbReference type="GeneID" id="30030019"/>
    <dbReference type="OrthoDB" id="347124at2759"/>
    <dbReference type="UniPathway" id="UPA00768"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000247">
        <property type="term" value="F:C-8 sterol isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006716">
        <property type="entry name" value="ERG2_sigma1_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868">
        <property type="entry name" value="SIGMA 1-TYPE OPIOID RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10868:SF1">
        <property type="entry name" value="SIGMA NON-OPIOID INTRACELLULAR RECEPTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04622">
        <property type="entry name" value="ERG2_Sigma1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291758" description="C-8 sterol isomerase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="216" mass="24167" checksum="AB5FFD874F7F10C1" modified="2016-10-05" version="1">MWIKLVTILVAVYAVLEASFQTWLPAHFRFDKQVLQDLVQETLQAHPNGNATQIMTDLAPRIQRAYPGLINDLNFDDWVYNNAGGAMGTMFILHASISEYLIFFGTAVGTEGHTGVHYADDYFTILTGHQLAADPCSLTPEIFYPGDQHHLKKGHVKQYAMPSGGFALELAQGWIPAMLPFGFVSVASLTLDFHSFYRTVYFTARDMLKNLAQGKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDZ1</accession>
    <name>A0A1A0HDZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">E3 ubiquitin-protein ligase</fullName>
            <ecNumber evidence="10">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_148750</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Ubiquitin ligase protein which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the E3 ubiquitin-protein ligase UBR1-like family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712727.1">
        <property type="nucleotide sequence ID" value="XM_018854553.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HDZ1"/>
    <dbReference type="GeneID" id="30027529"/>
    <dbReference type="OrthoDB" id="26387at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19673">
        <property type="entry name" value="UBR-box_UBR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.30:FF:000002">
        <property type="entry name" value="Putative e3 ubiquitin-protein ligase ubr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044046">
        <property type="entry name" value="E3_ligase_UBR-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039164">
        <property type="entry name" value="UBR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055194">
        <property type="entry name" value="UBR1-like_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003126">
        <property type="entry name" value="Znf_UBR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497:SF26">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21497">
        <property type="entry name" value="UBIQUITIN LIGASE E3 ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18995">
        <property type="entry name" value="PRT6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22960">
        <property type="entry name" value="WHD_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02207">
        <property type="entry name" value="zf-UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00396">
        <property type="entry name" value="ZnF_UBR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51157">
        <property type="entry name" value="ZF_UBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBR-type" evidence="11">
        <location>
            <begin position="83"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="UBR-type" evidence="9">
        <location>
            <begin position="83"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1740" mass="198293" checksum="B07890E02E8C9D87" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE21</accession>
    <name>A0A1A0HE21_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin C-terminal-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_27733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712757.1">
        <property type="nucleotide sequence ID" value="XM_018855341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE21"/>
    <dbReference type="STRING" id="869754.A0A1A0HE21"/>
    <dbReference type="GeneID" id="30028317"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF17">
        <property type="entry name" value="FI21816P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="2">
        <location>
            <begin position="244"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="521"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="676"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="700" mass="76206" checksum="3F63E5B0627C9C6F" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE51</accession>
    <name>A0A1A0HE51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-terminal acetyltransferase A, auxiliary subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39532</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MDM20/NAA25 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712761.1">
        <property type="nucleotide sequence ID" value="XM_018857151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE51"/>
    <dbReference type="STRING" id="869754.A0A1A0HE51"/>
    <dbReference type="GeneID" id="30030127"/>
    <dbReference type="OrthoDB" id="1874341at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019183">
        <property type="entry name" value="NAA25_NatB_aux_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767:SF3">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 25, NATB AUXILIARY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22767">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09797">
        <property type="entry name" value="NatB_MDM20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="760" mass="88701" checksum="95A7292D9A85073A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HED9</accession>
    <name>A0A1A0HED9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoglycerate mutase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_31193</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712763.1">
        <property type="nucleotide sequence ID" value="XM_018855801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HED9"/>
    <dbReference type="STRING" id="869754.A0A1A0HED9"/>
    <dbReference type="GeneID" id="30028777"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004331">
        <property type="term" value="F:fructose-2,6-bisphosphate 2-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
        <property type="term" value="P:negative regulation of glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043456">
        <property type="term" value="P:regulation of pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051695">
        <property type="entry name" value="Phosphoglycerate_Mutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46517:SF1">
        <property type="entry name" value="FRUCTOSE-2,6-BISPHOSPHATASE TIGAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="226" mass="25267" checksum="0DE70F05E5FE0380" modified="2016-10-05" version="1">MTREIKPNEDPQVTRVFVVRHGRTDWNAKKILQGHIDIDINEEGAQQAQKVASHLQGVRIDDLVSSDLCRCVNTSKPILSYQHTATYSETPKLRERDMGKVQGMPLIDALQTYGENFRSLGEQESALIERVSEVYDSALKRAASNKHKNILLCTHGGVITVFTNHLHRDRKYCLGEGITADDLRVPYNTSITVIDVEKATGSGTIVNCGVTEHLGGDFRVKNQLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEF6</accession>
    <name>A0A1A0HEF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_86961</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712872.1">
        <property type="nucleotide sequence ID" value="XM_018859271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEF6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEF6"/>
    <dbReference type="GeneID" id="30032246"/>
    <dbReference type="OrthoDB" id="2735536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05227">
        <property type="entry name" value="AR_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050425">
        <property type="entry name" value="NAD(P)_dehydrat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366:SF579">
        <property type="entry name" value="3-BETA HYDROXYSTEROID DEHYDROGENASE_ISOMERASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_3G02250)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10366">
        <property type="entry name" value="NAD DEPENDENT EPIMERASE/DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="7"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="342" mass="37005" checksum="98780AE8B3AF5730" modified="2016-10-05" version="1">MTVDETVFVTGATGFIGAHCVGLLLLQNYRVKAYCRSKEKFDRLARCVPETRRSFLEPAFGSDITNALELATLMAECTGVLHLASPFTFEVASFVDDLLLPALQGTKAVLKAALANASVNRVVITSSFAAVYDASKGDQPGVVLDETDFSPLTWQDGATTKNPAVAYRASKTVAENAAWKYMEQHKPNFSLTVLCPTMVFGPLFSPSLISGTQELNFSNQVVWGLLKGSGQVPPTKNPVWVDVRDLAEYHVKALSAPNAANQRFLISAGDYDNQEIADILRENLPLKITKTVPVGNPGSRIKGSHYTTNSSKGVKMLGVSYRALETCVLDLAKQLVAIDSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEI2</accession>
    <name>A0A1A0HEI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Defect at low temperature protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DLT1</name>
        <name evidence="8" type="ORF">METBIDRAFT_16974</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Required for growth under high-pressure and low-temperature conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DLT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712891.1">
        <property type="nucleotide sequence ID" value="XM_018854675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEI2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEI2"/>
    <dbReference type="GeneID" id="30027651"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038869">
        <property type="entry name" value="DLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021:SF1">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="27501" checksum="7D35DBBCBF63D23B" modified="2016-10-05" version="1" fragment="single">RVLEWLYSFSLAILLLFTLALVVVTPVDVIIQTQGALSLGLKLFIIIGAHALFLFLALVYYFSRLFNTRVLINQIPAKSVYLPLEPHDLPEPTRAHITANLRRCLGDIRVRAGPLNNPTERFDYAGRAPPLYIQQRNVRLGFANEISGLPENCVFQDVVNSIGLKIKADGLFAGSFTIPRHYTFREIILAMVQEIEDEGHLDDDLATTARLVVAAYERFKFGRAPIRHADLTAFLARLEQLMNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEJ0</accession>
    <name>A0A1A0HEJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial</fullName>
            <ecNumber evidence="4">2.1.1.201</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ubiquinone biosynthesis methyltransferase COQ5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">COQ5</name>
        <name evidence="6" type="ORF">METBIDRAFT_38943</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Methyltransferase required for the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2-methoxy-6-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-methionine = a 5-methoxy-2-methyl-3-(all-trans-polyprenyl)benzene-1,4-diol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28286"/>
            <dbReference type="Rhea" id="RHEA-COMP:10858"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84166"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="EC" id="2.1.1.201"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion inner membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
            <orientation evidence="4">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily. MenG/UbiE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.201" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713025.1">
        <property type="nucleotide sequence ID" value="XM_018857072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEJ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEJ0"/>
    <dbReference type="GeneID" id="30030048"/>
    <dbReference type="OrthoDB" id="6329284at2759"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031314">
        <property type="term" value="C:extrinsic component of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000133">
        <property type="entry name" value="2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01813">
        <property type="entry name" value="MenG_UbiE_methyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004033">
        <property type="entry name" value="UbiE/COQ5_MeTrFase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023576">
        <property type="entry name" value="UbiE/COQ5_MeTrFase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01934">
        <property type="entry name" value="MenG_MenH_UbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01209">
        <property type="entry name" value="Ubie_methyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51608">
        <property type="entry name" value="SAM_MT_UBIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01183">
        <property type="entry name" value="UBIE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01184">
        <property type="entry name" value="UBIE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="4" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="162"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="293" mass="32563" checksum="009D675DBDC9B0FE" modified="2016-10-05" version="1">MLARTYAKPTGSPQTAGEASATEPAKKNTDLATTHFGFRTVNKEDKEKMVRGVFSSVASKYDVMNDAMSFGVHRLWKHHFINRLDAGMRASSSKPLDFLDVAGGTGDIAFGLLEHAEQKFGDTKSTMTVADINPDMLAEGRVRYEKSKWNDGSNRVSFLEQNGETMESIPDESKDVYTIAFGIRNFTNIQAGLNTAYRVLKPGGIFACLEFSHVENPVVDYVYQAYSFGALPLMGQLIADDRDSYQYLVESIQRFPKQEAFRGMIEKAGFYVPEQGYENLTFGVASIHIGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HET7</accession>
    <name>A0A1A0HET7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glucan endo-1,3-beta-D-glucosidase</fullName>
            <ecNumber evidence="3">3.2.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_77810</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;3)-beta-D-glucosidic linkages in (1-&gt;3)-beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 81 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.39" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712898.1">
        <property type="nucleotide sequence ID" value="XM_018858772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HET7"/>
    <dbReference type="STRING" id="869754.A0A1A0HET7"/>
    <dbReference type="GeneID" id="30031748"/>
    <dbReference type="OrthoDB" id="4473401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052861">
        <property type="term" value="F:endo-1,3(4)-beta-glucanase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042973">
        <property type="term" value="F:glucan endo-1,3-beta-D-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1170">
        <property type="entry name" value="glycoside hydrolase family 81 endo-[beta] glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005200">
        <property type="entry name" value="Endo-beta-glucanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040720">
        <property type="entry name" value="GH81_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040451">
        <property type="entry name" value="GH81_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983">
        <property type="entry name" value="ENDO-1,3(4)-BETA-GLUCANASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31983:SF20">
        <property type="entry name" value="GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17652">
        <property type="entry name" value="Glyco_hydro81C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03639">
        <property type="entry name" value="Glyco_hydro_81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52008">
        <property type="entry name" value="GH81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 81 N-terminal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 81 C-terminal" evidence="10">
        <location>
            <begin position="208"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="582" mass="66085" checksum="00960C1580D2F490" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEZ6</accession>
    <name>A0A1A0HEZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sorting nexin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_39203</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Prevacuolar compartment membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713046.1">
        <property type="nucleotide sequence ID" value="XM_018857111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEZ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEZ6"/>
    <dbReference type="GeneID" id="30030087"/>
    <dbReference type="OrthoDB" id="5227681at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030904">
        <property type="term" value="C:retromer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034499">
        <property type="term" value="P:late endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051074">
        <property type="entry name" value="Sorting_Nexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45963:SF2">
        <property type="entry name" value="RE52028P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="13">
        <location>
            <begin position="29"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="154" mass="18025" checksum="DD9F95D3F3738E48" modified="2016-10-05" version="1">MSDSFRPVSEVLSNSHKKQSFNDIYGEPENFLEIEVCNPQTHLGREQYTDYEIVCRTNIPAFKKRQSRVRRRYSDFVTLKKILEQELTRVIIPPLPGKILLSLNKFNDLNIESRRQGLEKFLAVVSGHPLLQTGSSTLVEFIQNDRWEPRYHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFB7</accession>
    <name>A0A1A0HFB7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cora-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_16442</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713172.1">
        <property type="nucleotide sequence ID" value="XM_018854643.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFB7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFB7"/>
    <dbReference type="GeneID" id="30027619"/>
    <dbReference type="OrthoDB" id="29879at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990816">
        <property type="term" value="C:vacuole-mitochondrion membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010961">
        <property type="term" value="P:intracellular magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12829">
        <property type="entry name" value="Alr1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000008">
        <property type="entry name" value="CorA family metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044089">
        <property type="entry name" value="Alr1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORT PROTEIN/MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21535:SF51">
        <property type="entry name" value="MANGANESE RESISTANCE PROTEIN MNR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="723"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="758"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="252"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="522"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="785" mass="88424" checksum="4D2A2354DB15E182" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFD8</accession>
    <name>A0A1A0HFD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS5m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_39428</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713108.1">
        <property type="nucleotide sequence ID" value="XM_018857139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFD8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFD8"/>
    <dbReference type="GeneID" id="30030115"/>
    <dbReference type="OrthoDB" id="309483at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000022">
        <property type="entry name" value="28S ribosomal protein S5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000041">
        <property type="entry name" value="37S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48277:SF1">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOMAL PROTEIN S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="10">
        <location>
            <begin position="173"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="37897" checksum="55E4212705532E80" modified="2016-10-05" version="1">MFKSGFSRVLLKTPAHRFLTCGRPVLQAPLPTKASNAHKAAEHMEFLLKFYSPELLQSVRIAESLVSPDEYLALRKKGPKALSRVGLTNESQDFSHNDPEWEEPIIYPNQATDHTPYARIPQIRAPDRSDLQLTFSLEDIKKSRPNTMGASRDKFFQELHQLTGMEERYMRSLYVRPIVMKRVSCQTSKGKIPNFYALTVVGDKNGMIGLGEGKSRDGMRVSLRKAHWNAIKNLQPIPRYENRTIIGDVDYKFHAVKLFLKSAPAGFGLRVNPNIFEVCQAAGIKDLRGKVYKSRNPMNVVKGFVEALTKQTSLEDLAAGRGKKLVDLRKVYYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFD9</accession>
    <name>A0A1A0HFD9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H3-like centromeric protein CSE4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CENP-A homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">CENPA homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_9072</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes. Replaces conventional H3 in the nucleosome core of centromeric chromatin that serves as an assembly site for the inner kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of centromeric nucleosomes, where DNA is wrapped around a histone octamer core. The octamer contains two molecules each of H2A, H2B, CSE4/CENPA and H4 assembled in one CSE4-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with the inner kinetochore.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713181.1">
        <property type="nucleotide sequence ID" value="XM_018859309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFD9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFD9"/>
    <dbReference type="GeneID" id="30032284"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005729">
        <property type="term" value="C:2-micrometer circle DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043505">
        <property type="term" value="C:CENP-A containing nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030543">
        <property type="term" value="P:2-micrometer plasmid partitioning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061644">
        <property type="term" value="P:protein localization to CENP-A containing chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000087">
        <property type="entry name" value="Probable histone 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="16">
        <location>
            <begin position="47"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="141" mass="15945" checksum="801732A0502AF937" modified="2016-10-05" version="1">MARLSTNPNRLAAGPRTPRPTGETGFYRNLPGDPPLNAGARKRRYRPGTNALREIRKFQRGTDLLLRKLPFARLVKEIADIYIGSGYGIRWQSNAILALQEASEAFLIHLLEDTNLCAIHAKRVTIMQKDIQLARRIRGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFJ2</accession>
    <name>A0A1A0HFJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">dihydroorotase</fullName>
            <ecNumber evidence="4">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_34966</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class II DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713236.1">
        <property type="nucleotide sequence ID" value="XM_018856548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFJ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFJ2"/>
    <dbReference type="GeneID" id="30029524"/>
    <dbReference type="OrthoDB" id="1670005at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01294">
        <property type="entry name" value="DHOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000041">
        <property type="entry name" value="Dihydroorotase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00219">
        <property type="entry name" value="PyrC_classII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004721">
        <property type="entry name" value="DHOdimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00856">
        <property type="entry name" value="pyrC_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43137:SF1">
        <property type="entry name" value="DIHYDROOROTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001237">
        <property type="entry name" value="DHOdimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="9">
        <location>
            <begin position="83"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="359" mass="39160" checksum="52B3A82104CCA6AE" modified="2016-10-05" version="1">MTEIDLGITADMHVHLRDGKMMELVTPTVRQGGVSVAYVMPNLAPPVTTLERVQRYRKDLQALAPKTTFLMSLYLCKDLTPELIHAAAKAGAIHGVKCYPAGVTTNSSAGVDPNDFTPFYPVFEALQAEDLVLNLHGEKPPVKDGDIHVLNAEPEFLPALVKLARDFPKLKIILEHCTTKAAVDTVRKLHQENPACRVAATITAHHLSLTIDSWAGNPINFCKPVAKLPADMRALVDAATSGELYFFFGSDLAPHPIESKAKHVGVCAGVYTQLHAIAYLAEVFESAGKLEHLVKFVSENGKAFYGIKDADLVSKDRAVLVARENQVPEEIGNSSLTVVPFKAGDNLKYAVEWRECGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFP4</accession>
    <name>A0A1A0HFP4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 18">Delta(24(24(1)))-sterol reductase</fullName>
            <ecNumber evidence="15 18">1.3.1.71</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">C-24(28) sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sterol Delta(24(28))-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_75910</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ergosterol + NADP(+) = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16933"/>
            <dbReference type="ChEBI" id="CHEBI:18249"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.71"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:18503"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="17">Steroid metabolism; ergosterol biosynthesis; ergosterol from zymosterol: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.71" evidence="15 18"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713198.1">
        <property type="nucleotide sequence ID" value="XM_018858444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFP4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFP4"/>
    <dbReference type="GeneID" id="30031420"/>
    <dbReference type="OrthoDB" id="5326588at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000246">
        <property type="term" value="F:Delta24(24-1) sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050614">
        <property type="term" value="F:Delta24-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000003">
        <property type="entry name" value="C-24(28) sterol reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF31">
        <property type="entry name" value="DELTA(24(24(1)))-STEROL REDUCTASE ERG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="9 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="10 18" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="13 18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="403"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA22717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="460" mass="53506" checksum="6426D40C52507924" modified="2016-10-05" version="1">MSQSEAAPLVDASEGKYLPKDQIEWEFGGPAGALGMMIGFPLLMVYMWISAQFYGGQPAWPADEQTWTDFGEQLWQYFVQHGVPSLTVVAVFTVFFVVQGVFYLVLPGVWTKGQPLTHLGGKQLPYYCNAVWSFYTTNVVLIGMHFAGYKMYFIIDHFGEIMVTAIVYGLVFSIGLYVYTLTVSKDYHRMTGNHIYDCFMGAPLNPRIGKYLDIKMFFEVRIPWFTLYFLSLATVLKQFDTYGHASPQAWFLLYAHWLYANACSKGEELIVPTWDMAYEKFGFMLCFWNIAGVPYTYCHCTLFVAYHDPSEYRWSTMYNVSLFAVLTVAYFFFDTGNRQKNSFRRIMSGNTNLRKTFPYLPCSDLVNPSYIRCENGSLLLTDGWYKYARKAHYTADYVQSLTWGLICGFASPFPWFFPVFFLIVLVHRAYRDQRKCARKYGKDWDRYLEACPYMFIPYVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG01</accession>
    <name>A0A1A0HG01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcium-binding protein NCS-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_75962</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713264.1">
        <property type="nucleotide sequence ID" value="XM_018858455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG01"/>
    <dbReference type="STRING" id="869754.A0A1A0HG01"/>
    <dbReference type="GeneID" id="30031431"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000009">
        <property type="entry name" value="Visinin-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="191" mass="22215" checksum="5E9F7FD3FDCEE726" modified="2016-10-05" version="1">MGKAVSKLSKEEIKQLRDRTLFDKRELQQWYKGFLRDCPSGHLSEEEFAKVFKQFFPFGDPTDYCHYLFRIFDTDGSKYIDFKEFIVALSLTARGDLNQKLDFTFKLYDLDRDGKIYYQDVLAVVTAIYKMVGPMVALPEDERTPDLRAAKLFLAIDKDKDSDFLDFDDLKRVVKADPSLINSLNSYEGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG64</accession>
    <name>A0A1A0HG64_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_34545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713461.1">
        <property type="nucleotide sequence ID" value="XM_018856484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG64"/>
    <dbReference type="STRING" id="869754.A0A1A0HG64"/>
    <dbReference type="GeneID" id="30029460"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053051">
        <property type="entry name" value="HDAC_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47793:SF1">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT CTI6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20826">
        <property type="entry name" value="PHD_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="5">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="139"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="221"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="355" mass="40527" checksum="0AA59C1B350F7E10" modified="2016-10-05" version="1">MSRRSGRAKQSATESSRTPEIDNAPELDEQAEVTRCICGNDEIASINTSLQRLLLLEYSIKIDNGLFIQCDKCLVWQHGYCVGLFIDNDVPDKYWCEQCKPSLHMLVTDNEDRTSRTLYRPVNDGRDALLKFLTEKESGAAKPADSDSTLSHTLSNRQTRKERRHVEDSFDEQLQRALRESAASTERKRNAQTDSAQSNKRSHSEDLENDVNDSNLDDDTDLKTAKRDSRVKSKLRAKQKARSQSSRGLASQPKSEADSLVVTKEELLSQPLQPRFVNEKSTIYELRKRTGAILEWLGRSQMELEEERDVKTLQFKEANVEGANLLLLVEENSKLMENLTEKILAWEQRFGKYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGA8</accession>
    <name>A0A1A0HGA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sec1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713374.1">
        <property type="nucleotide sequence ID" value="XM_018855409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGA8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGA8"/>
    <dbReference type="GeneID" id="30028385"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="692" mass="78944" checksum="1A128A8C5A79E570" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGB3</accession>
    <name>A0A1A0HGB3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HSP20-like chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_29727</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the p23/wos2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713685.1">
        <property type="nucleotide sequence ID" value="XM_018855467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGB3"/>
    <dbReference type="GeneID" id="30028443"/>
    <dbReference type="OrthoDB" id="1564555at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06465">
        <property type="entry name" value="p23_hB-ind1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000013">
        <property type="entry name" value="Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045250">
        <property type="entry name" value="p23-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932:SF1">
        <property type="entry name" value="CO-CHAPERONE PROTEIN DAF-41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22932">
        <property type="entry name" value="TELOMERASE-BINDING PROTEIN P23 HSP90 CO-CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="221" mass="23516" checksum="C6D52F2BA1747265" modified="2016-10-05" version="1">MAVHPVVLWAQRSSESEPAKNVVYLTIQVQDPVDVKLDLSATSLKFTAQSSDKATDYELNLDFFDEIDPENSYKHESGSHILYVLRKKTAQAEFWPRLLKEKLKLHYIKTDFDKWVDEDEQDEQAEEEDPMAAMGGMGGMPGMEGMGGMPGMGGMPGMGGMPGMGGPGGMDFSQLLAGAGGAGGAGGDFDISALASQLGQNAAAGDDDGDDEGDEGVEETK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGB9</accession>
    <name>A0A1A0HGB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Actin-binding FH2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_35441</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell septum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the formin homology family. BNI1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713532.1">
        <property type="nucleotide sequence ID" value="XM_018856608.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HGB9"/>
    <dbReference type="GeneID" id="30029584"/>
    <dbReference type="OrthoDB" id="1104827at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000142">
        <property type="term" value="C:cellular bud neck contractile ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001411">
        <property type="term" value="C:hyphal tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903475">
        <property type="term" value="P:mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000920">
        <property type="term" value="P:septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000006">
        <property type="entry name" value="Cytokinesis protein sepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.30.50:FF:000001">
        <property type="entry name" value="Cytokinesis sepA protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000898">
        <property type="entry name" value="Formin BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.150">
        <property type="entry name" value="Formin, FH3 diaphanous domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051661">
        <property type="entry name" value="Actin_filament_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47102:SF2">
        <property type="entry name" value="PROTEIN BNI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="8">
        <location>
            <begin position="4"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="9">
        <location>
            <begin position="994"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="7">
        <location>
            <begin position="1425"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="645"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="796"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="869"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1410"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1463"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1514"/>
            <end position="1565"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="473"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="873"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="890"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="910"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="931"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="964"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1416"/>
            <end position="1430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1554"/>
            <end position="1565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1565" mass="178223" checksum="B8F989480C310117" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGD4</accession>
    <name>A0A1A0HGD4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SGNH hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713547.1">
        <property type="nucleotide sequence ID" value="XM_018856507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGD4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGD4"/>
    <dbReference type="GeneID" id="30029483"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF19">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="254" mass="28790" checksum="F3C8794273F2F96C" modified="2016-10-05" version="1">MPLFFDKFVLFGDSITEYSNKQDGFALAPGLQDLYSRKMDIVTRGFSGYNTDQGLVVLREVLKAENAIPNSVKLMYIFMGTNDAASTFQGVPVDRYRLNLSRMVQLAASQGIKVIVVGPALHDQALVSEMREERGEPEQAHFSSNENTRKYADTALEVAAENGVPFVDLWSEFQKYGEWSTEDLLANKPDLAELLVDGIHYTPKAYEVFYDALVETIVKHYPDLARLSLKLRLPYYRDVDYGNIEESLMKSMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGR4</accession>
    <name>A0A1A0HGR4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_34526</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713661.1">
        <property type="nucleotide sequence ID" value="XM_018856481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGR4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGR4"/>
    <dbReference type="GeneID" id="30029457"/>
    <dbReference type="OrthoDB" id="5566198at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000578">
        <property type="entry name" value="Nucleoporin seh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF3">
        <property type="entry name" value="NUCLEOPORIN SEH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="5"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="277"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="349" mass="39510" checksum="71C9C1750EF4DBC8" modified="2016-10-05" version="1">MRAFQTGHEELVHDIKYDFYGRHVATASSDQHVKVFDLDLATQTWVLNDSWKAHDSLVVRLLWAHPEFSSFKILALCSYDRTVKIWQEMPDELQGLGRRWYKLATLAVELFGPIYDVQFAPPHLGLKLACVGSDGIVRVYQLVEPADLLVWELCAEIPILDLQLPAKSLQSSFSIEWCPSKFAKTEKFAVIALDQGFAARSSDLLDEPSGPSERTPRYVKVCSLPEHNGLIRLVSWAPSMGRSHHLLATGCKDGYIRIFKAFETASGDLRVETVAKLNDHRQEVWRVEWNATGTILSSAGDDGKVRLWKCNYMNEWQCMSEINTSNRQDARAVLSADDPMYLLRPPASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGV8</accession>
    <name>A0A1A0HGV8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ARM repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_76283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713712.1">
        <property type="nucleotide sequence ID" value="XM_018858505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGV8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGV8"/>
    <dbReference type="GeneID" id="30031481"/>
    <dbReference type="OrthoDB" id="27832at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="2">
        <location>
            <begin position="357"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G" evidence="2">
        <location>
            <begin position="562"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="806"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="886"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1005" mass="112219" checksum="781CD079A2088322" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGV9</accession>
    <name>A0A1A0HGV9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP synthase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_36046</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713598.1">
        <property type="nucleotide sequence ID" value="XM_018856684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGV9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGV9"/>
    <dbReference type="GeneID" id="30029660"/>
    <dbReference type="OrthoDB" id="9805536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18116">
        <property type="entry name" value="ATP-synt_F1_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1-ATPase_alpha_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.150.20:FF:000001">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000001">
        <property type="entry name" value="ATP synthase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004039">
        <property type="entry name" value="ATP synthase subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="entry name" value="ATP synthase alpha/beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a_nt-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009884">
        <property type="entry name" value="PRK13343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48082:SF2">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 12" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="15">
        <location>
            <begin position="61"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="13">
        <location>
            <begin position="183"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase alpha subunit C-terminal" evidence="14">
        <location>
            <begin position="413"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA23117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="542" mass="58240" checksum="F78FF37B7EA78283" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH17</accession>
    <name>A0A1A0HH17_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_9445</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713624.1">
        <property type="nucleotide sequence ID" value="XM_018859392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH17"/>
    <dbReference type="STRING" id="869754.A0A1A0HH17"/>
    <dbReference type="GeneID" id="30032367"/>
    <dbReference type="OrthoDB" id="298012at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030267">
        <property type="term" value="F:glyoxylate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050223">
        <property type="entry name" value="D-isomer_2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996:SF178">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE YGL185C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10996">
        <property type="entry name" value="2-HYDROXYACID DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase catalytic" evidence="4">
        <location>
            <begin position="60"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding" evidence="5">
        <location>
            <begin position="185"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="364" mass="39449" checksum="7DAD215B2442E603" modified="2016-10-05" version="1">MTRPKVLILGDLNTSLPHYKRFLEKFECIHHTLASTREEVIADFHTKFHDISAIYGAWLGFVLVGGFHSDIIEALPPTLKVVSICSVGHDNYNGAQMKEKGVVLTNVPSDGAAEPVADLVLYNALTAFRNFNMSLAFLRPENDHTVAVRAELESCKFDFQTGALVGGLPQGYSFGEYIGGRPCLSPKGHNAVIVGFGNIGQTIGRKLSLIGMNIHYVKRRQLSGAEERSLGFVATYHRSIGATCLFADLVVIACPATPETHHLLNSAVLGSFAKPIRVINIGRGSVIDEQALVDSLKSGKVVFAGLDVYENEPSVHPELYGRQDVFLTPHVGASTTENFDHTAVVAMENIEAVLGGRPALTQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHN7</accession>
    <name>A0A1A0HHN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_107159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714001.1">
        <property type="nucleotide sequence ID" value="XM_018853831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHN7"/>
    <dbReference type="GeneID" id="30026807"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="111" mass="12562" checksum="54607991D0522713" modified="2016-10-05" version="1">MPVYRLLERGVSIRGRAIWRALMPNTQGRLASGVFAMRSGICSTECFFPLLHHVWVLLCNSVPRYICEDFAAPGGYTALYLADRPVVLRTLLEDKRLAPKLVNNTAENWLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHQ3</accession>
    <name>A0A1A0HHQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RecQ mediated genome instability protein 1 OB-fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_76668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714170.1">
        <property type="nucleotide sequence ID" value="XM_018858568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HHQ3"/>
    <dbReference type="GeneID" id="30031544"/>
    <dbReference type="OrthoDB" id="341511at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.770">
        <property type="entry name" value="RecQ-mediated genome instability protein Rmi1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042470">
        <property type="entry name" value="RMI1_N_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013894">
        <property type="entry name" value="RMI1_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08585">
        <property type="entry name" value="RMI1_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecQ mediated genome instability protein 1 OB-fold" evidence="1">
        <location>
            <begin position="21"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="189" mass="21581" checksum="37874F1402B4AB4F" modified="2016-10-05" version="1">MNQEKSADVASGITQYFGLPEAELKEPLVCHVMQILNISESKEKALEEWRYYQNPNTAPFERMEHVYRPIIYGIDLETPEPEQKARSVKATYKLLLRDCFGNYFYAIELEELPFLRPGTNTTKTPLPIPLGGCITLGKGTLIADGFVLMKKHLCTYQEPDPFSELTKSLNENLVGKNIEMIEHLLNDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHQ4</accession>
    <name>A0A1A0HHQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Dynactin subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_107315</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the dynactin subunits 5/6 family. Dynactin subunit 5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714017.1">
        <property type="nucleotide sequence ID" value="XM_018853838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHQ4"/>
    <dbReference type="GeneID" id="30026814"/>
    <dbReference type="OrthoDB" id="417208at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005869">
        <property type="term" value="C:dynactin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047125">
        <property type="entry name" value="DCTN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46126:SF1">
        <property type="entry name" value="DYNACTIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21711">
        <property type="entry name" value="DCTN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="177" mass="18947" checksum="3A2A8CE07E62A0A7" modified="2016-10-05" version="1">MDDWIETGTGNRISRLAIIKGANRISILENCTISENCTLNGNVATLSPSLPSIVLGKYTYLGCRCLIDPPRANPSPDASVFTDVKVGNYTTLGADSVVRLIQVGNRVLIGARCVLGELSVINDCCVIEDDTVVPANSVVPPYSRVSGVPGIDYSVVPISASYRKVLEADSRVRHVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHT2</accession>
    <name>A0A1A0HHT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_37785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713884.1">
        <property type="nucleotide sequence ID" value="XM_018856914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHT2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHT2"/>
    <dbReference type="GeneID" id="30029890"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF10">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="25"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="807" mass="90995" checksum="BA7E5B50B3A860FD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHW0</accession>
    <name>A0A1A0HHW0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tyrosine specific protein phosphatases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_85689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713948.1">
        <property type="nucleotide sequence ID" value="XM_018859251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHW0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHW0"/>
    <dbReference type="GeneID" id="30032226"/>
    <dbReference type="OrthoDB" id="449382at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF1">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="1">
        <location>
            <begin position="478"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="648" mass="72798" checksum="DF0F8CD771BFD67B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHW5</accession>
    <name>A0A1A0HHW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">VPS37 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_35497</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713953.1">
        <property type="nucleotide sequence ID" value="XM_018856615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHW5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHW5"/>
    <dbReference type="GeneID" id="30029591"/>
    <dbReference type="OrthoDB" id="10260857at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000813">
        <property type="term" value="C:ESCRT I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072666">
        <property type="term" value="P:establishment of protein localization to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037202">
        <property type="entry name" value="ESCRT_assembly_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009851">
        <property type="entry name" value="Mod_r"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07200">
        <property type="entry name" value="Mod_r"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140111">
        <property type="entry name" value="Endosomal sorting complex assembly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51314">
        <property type="entry name" value="VPS37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0967">Endosome</keyword>
    <keyword evidence="5 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="VPS37 C-terminal" evidence="7">
        <location>
            <begin position="97"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="21298" checksum="E4EDCD1447341831" modified="2016-10-05" version="1">MNEEPQAFTLEALQPFPLEKSLQSLPQQFRDEFASLYELVKGYVRNLKLYQDLEKQLRDAVNDTISTINSIIRLLEEYESHAAIISEKAERLDRLYKDFLTLETLQYQLLSSNFDQTFLKAKFRNLVASSDMKSGEIIGSYKENGGDMTQFLLDFKNSRKLYHARREKLHRWDEERVSGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHZ5</accession>
    <name>A0A1A0HHZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S ribosomal protein L18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76619</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714111.1">
        <property type="nucleotide sequence ID" value="XM_018858561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHZ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHZ5"/>
    <dbReference type="GeneID" id="30031537"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="3"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="330" mass="36400" checksum="6881CD8C72B9E21A" modified="2016-10-05" version="1">MGKDHTKKQHVRVSHRTAPKSDNVYLNLLVKLYTYLARRTDAPFNKVVLKSLFLSKVNRPPVSLTKINSVVSQKGAAEKTVVVVGTVTDDLRLLEVPKVTVAALRFTAGAKARIVKAGGEAITLDQLALRAPKGQNTVLVRGPRNARQAVRHFGFGPHKGKAPKVISKGRKFERARGKRNSRGFKMPGVTVKDVPAQDFINAYAQFLQRQGKLEVPGYVELVKTSAGNELPPQESETWFYKRAASAARHVYMRKNVGVGKLNKLYGGAINRGFRPQKHADASGSVNRRVVQALEKIGVLEISPKGGRRISENGQRDLDRIAAQTLEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI09</accession>
    <name>A0A1A0HI09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_76492</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713959.1">
        <property type="nucleotide sequence ID" value="XM_018858538.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HI09"/>
    <dbReference type="GeneID" id="30031514"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000130">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000001">
        <property type="entry name" value="Phospholipid-transporting ATPase IC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
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        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
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    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF180">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE DNF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="150"/>
            <end position="170"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="439"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1068"/>
            <end position="1087"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1099"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1157"/>
            <end position="1176"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1188"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1215"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1255"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="19">
        <location>
            <begin position="64"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="18">
        <location>
            <begin position="275"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="20">
        <location>
            <begin position="1036"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="14">
        <location>
            <position position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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            <position position="553"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <position position="554"/>
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            <name>ATP</name>
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            <name>ATP</name>
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        <location>
            <position position="689"/>
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            <name>ATP</name>
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        <location>
            <position position="730"/>
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            <name>ATP</name>
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        <location>
            <position position="753"/>
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        <ligand>
            <name>ATP</name>
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        <location>
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            <name>ATP</name>
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            <name>ATP</name>
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            <dbReference type="ARBA" id="ARBA00008109"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA23478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1408" mass="159774" checksum="7820CCB107E47B7B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI15</accession>
    <name>A0A1A0HI15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Adenylyl cyclase-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30087</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The N-terminal domain binds to adenylyl cyclase, thereby enabling adenylyl cyclase to be activated by upstream regulatory signals, such as Ras. The C-terminal domain is required for normal cellular morphology and growth control.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the CAP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018855551.1"/>
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    <dbReference type="OrthoDB" id="77251at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008179">
        <property type="term" value="F:adenylate cyclase binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010619">
        <property type="term" value="P:adenylate cyclase-activating glucose-activated G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:cAMP-mediated signaling"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of cell growth"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of conjugation with cellular fusion"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090141">
        <property type="term" value="P:positive regulation of mitochondrial fission"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
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        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenylate cyclase-associated CAP, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001837">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013912">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013992">
        <property type="entry name" value="Adenylate_cyclase-assoc_CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018106">
        <property type="entry name" value="CAP_CS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053950">
        <property type="entry name" value="CAP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036222">
        <property type="entry name" value="CAP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10652:SF0">
        <property type="entry name" value="ADENYLYL CYCLASE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08603">
        <property type="entry name" value="CAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21938">
        <property type="entry name" value="CAP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01213">
        <property type="entry name" value="CAP_N-CM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101278">
        <property type="entry name" value="N-terminal domain of adenylylcyclase associated protein, CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01088">
        <property type="entry name" value="CAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="6">
        <location>
            <begin position="363"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="255"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="320"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="320"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00072052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="523" mass="56910" checksum="A8490F36EF480E48" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI40</accession>
    <name>A0A1A0HI40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_228644</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714310.1">
        <property type="nucleotide sequence ID" value="XM_018855209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI40"/>
    <dbReference type="STRING" id="869754.A0A1A0HI40"/>
    <dbReference type="GeneID" id="30028185"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="7">
        <location>
            <begin position="11"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="21296" checksum="F04C8442E0F294ED" modified="2016-10-05" version="1">MYFQDYGAGSGLGYLAVQYAKAIDYRVLAIDGGDDKSDFAKSLGAEAFVDFFKSLDVVKDVQEITNGGPHGVINGSVSEKAMQQFAEYVRATGTVILVGLPAGAKVVAPVFKSVVKSVNIKGSCVGNRADSAEAIDFLTRGLIKCPVKIVSLSELPSVYQLMARSWVVTWLTRRSRLAAKSWSGYRAGYEQKKKNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIF9</accession>
    <name>A0A1A0HIF9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NADH dehydrogenase (Ubiquinone) complex I, assembly factor 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_35621</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NDUFAF6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714435.1">
        <property type="nucleotide sequence ID" value="XM_018856634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIF9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIF9"/>
    <dbReference type="GeneID" id="30029610"/>
    <dbReference type="OrthoDB" id="270318at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21181:SF13">
        <property type="entry name" value="NADH DEHYDROGENASE (UBIQUINONE) COMPLEX I, ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="382" mass="43739" checksum="89E7DE84CA6D53B6" modified="2016-10-05" version="1">MLRGPNRVPYAVRYIVHSARTASYQLQLEKSVENIYALLEANDRLSYLLAQYIPEPARNAFIAIRAFALEINKITDGGSMDGRAAKALNQLSKSTGMTTADMKFKFWSELLVKSFSDKDDIGEPVAFLLRDALRNGLNLDIVYFHQFLQTRRHFLKSQQFSTVSDICSYGEGTYSQLNYATQSILLSPQISPSVIHMLELSTTLQSKVSDIAAHIGQATAVSSMILGMNFYASTRNQVTLPVNIMTKHELSQEALLRLSQGHTKNEGEIKDTRDRLKNVIFETAITANDHLLSARQKLKQVKEDIAEILEQRPRDTLLITNSKNWRKGLPDVIFVPFMLAIPTSLYLQRLEKHDFDVYSKKLQHKEWRLAWNSFRNYYQRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJA2</accession>
    <name>A0A1A0HJA2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_38298</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714399.1">
        <property type="nucleotide sequence ID" value="XM_018856986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJA2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJA2"/>
    <dbReference type="GeneID" id="30029962"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="84" mass="9111" checksum="ABA20F5C5AD974F2" modified="2016-10-05" version="1">MKPVVSTGKAYFCTILSAFAIVILSVIGYLFKIGHESMMGSINDPEDGEAVAKTVFGAVFVYLALFAFCGSQVCLIQKQNRIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF5</accession>
    <name>A0A1A0HJF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">L-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="15">1.1.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_77000</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19909"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19910"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the C-terminal section; belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">In the N-terminal section; belongs to the cytochrome b5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.3" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714626.1">
        <property type="nucleotide sequence ID" value="XM_018858619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJF5"/>
    <dbReference type="GeneID" id="30031595"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004460">
        <property type="term" value="F:L-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02922">
        <property type="entry name" value="FCB2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000062">
        <property type="entry name" value="Cytochrome b2, mitochondrial, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037396">
        <property type="entry name" value="FMN_HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008259">
        <property type="entry name" value="FMN_hydac_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037458">
        <property type="entry name" value="L-MDH/L-LDH_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578:SF148">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE (CYTOCHROME)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10578">
        <property type="entry name" value="S -2-HYDROXY-ACID OXIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00557">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51349">
        <property type="entry name" value="FMN_HYDROXY_ACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="18">
        <location>
            <begin position="26"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="FMN hydroxy acid dehydrogenase" evidence="19">
        <location>
            <begin position="130"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00066458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00068515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA24145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="543" mass="59848" checksum="06E2329190113E1F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJQ3</accession>
    <name>A0A1A0HJQ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_118153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714603.1">
        <property type="nucleotide sequence ID" value="XM_018854100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJQ3"/>
    <dbReference type="GeneID" id="30027076"/>
    <dbReference type="OrthoDB" id="540662at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846:SF0">
        <property type="entry name" value="PRE-MRNA PROCESSING FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="185"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="235"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="278"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="320"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="481"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="384"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="400"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="517" mass="55274" checksum="A445246F3475D060" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK48</accession>
    <name>A0A1A0HK48_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_38474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714743.1">
        <property type="nucleotide sequence ID" value="XM_018857007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK48"/>
    <dbReference type="STRING" id="869754.A0A1A0HK48"/>
    <dbReference type="GeneID" id="30029983"/>
    <dbReference type="OrthoDB" id="4356994at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053181">
        <property type="entry name" value="EcdB-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785:SF5">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47785">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="816"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="82"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="907" mass="101571" checksum="D8B15BF7E1FC9CBE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK69</accession>
    <name>A0A1A0HK69_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Pyruvate decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_38271</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>pyruvate + H(+) = acetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:45484"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714679.1">
        <property type="nucleotide sequence ID" value="XM_018856980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK69"/>
    <dbReference type="STRING" id="869754.A0A1A0HK69"/>
    <dbReference type="GeneID" id="30029956"/>
    <dbReference type="OrthoDB" id="3970464at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004737">
        <property type="term" value="F:pyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000949">
        <property type="term" value="P:aromatic amino acid family catabolic process to alcohol via Ehrlich pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02005">
        <property type="entry name" value="TPP_PDC_IPDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07038">
        <property type="entry name" value="TPP_PYR_PDC_IPDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1220:FF:000018">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000019">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000024">
        <property type="entry name" value="Pyruvate decarboxylase isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012110">
        <property type="entry name" value="PDC/IPDC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047214">
        <property type="entry name" value="TPP_PDC_IPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047213">
        <property type="entry name" value="TPP_PYR_PDC_IPDC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43452:SF30">
        <property type="entry name" value="PYRUVATE DECARBOXYLASE ISOZYME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036565">
        <property type="entry name" value="Pyruvt_ip_decrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="15">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="13">
        <location>
            <begin position="201"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="14">
        <location>
            <begin position="400"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>2</label>
            <note>allosteric activator</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <label>1</label>
            <note>substrate; ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036565-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA24198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="562" mass="61535" checksum="A74DB13A4848541C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKI1</accession>
    <name>A0A1A0HKI1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit CBF5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">H/ACA ribonucleoprotein complex subunit cbf5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_134835</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715016.1">
        <property type="nucleotide sequence ID" value="XM_018854373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKI1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKI1"/>
    <dbReference type="GeneID" id="30027349"/>
    <dbReference type="OrthoDB" id="10250002at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000454">
        <property type="term" value="P:snoRNA guided rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="10">
        <location>
            <begin position="17"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="9">
        <location>
            <begin position="266"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="356"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="408"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="473" mass="53710" checksum="49636BA7B8B253DA" modified="2016-10-05" version="1">MSEEHMIKPESVAPSKDTSEWPLLLKNYDKLLVRSGHYTPIPAGSSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRVEKTGHSGTLDPKVTGCLIVCIDRATRLVKSQQGAGKEYVCIVRLHEELEDAKEMGRALENLTGALFQRPPLISAVKRQLRVRTIYDSNLIEFDNKRGLGVFWASCEAGTYMRTLCVHLGMLLSVGGHMQELRRVRSGAMGENDNMVTLHDVMDAQYVYDNTRDESYLRKIIQPLETLLVGYKRIVVKDSAVNSVCYGAKFMIPGLLRYEEGIELYDEVVLMTTKGEAIAIGIAQMTTVDLQSCDHGVVAKVRRCIMERDTYPRRWGLGPVAQKKKQMKADGKLDKFGRANENTPETWKQEYKDHADEAAPAVPESKLVAPEVQLPKKSEPEVKSQETIVEKEEKEEKKEKKEKKDKKEKKDKKEKKEKKEKKRKADDDEEPEKKKKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKJ6</accession>
    <name>A0A1A0HKJ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RhoGAP-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_34249</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715031.1">
        <property type="nucleotide sequence ID" value="XM_018856453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKJ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKJ6"/>
    <dbReference type="GeneID" id="30029429"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060237">
        <property type="term" value="P:regulation of fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF25">
        <property type="entry name" value="F-BAR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="3">
        <location>
            <begin position="130"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="428"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="315"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="61317" checksum="F2A71AB6BDF70B45" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKK8</accession>
    <name>A0A1A0HKK8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_35575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715007.1">
        <property type="nucleotide sequence ID" value="XM_018856628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKK8"/>
    <dbReference type="STRING" id="869754.A0A1A0HKK8"/>
    <dbReference type="GeneID" id="30029604"/>
    <dbReference type="OrthoDB" id="76038at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38663:SF1">
        <property type="entry name" value="L-ORNITHINE N(5)-MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="1">
        <location>
            <begin position="14"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="561" mass="63336" checksum="6C74E2AED5E6C25E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKN0</accession>
    <name>A0A1A0HKN0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714844.1">
        <property type="nucleotide sequence ID" value="XM_018856448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKN0"/>
    <dbReference type="STRING" id="869754.A0A1A0HKN0"/>
    <dbReference type="GeneID" id="30029424"/>
    <dbReference type="OrthoDB" id="442460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019783">
        <property type="term" value="F:ubiquitin-like protein peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915:SF2">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46915">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="320"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="599"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="765"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="865"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="816"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="833"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="907"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="953"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="982"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1011" mass="116012" checksum="D21FAA15928F7083" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKP5</accession>
    <name>A0A1A0HKP5_9ASCO</name>
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        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_30663</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018855679.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HKP5"/>
    <dbReference type="GeneID" id="30028655"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1114"/>
            <end position="1213"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="1231"/>
            <end position="1384"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="1626"/>
            <end position="2126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="1062"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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        <location>
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            <end position="2469"/>
        </location>
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        </location>
    </feature>
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        <location>
            <begin position="2671"/>
            <end position="2694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="1090"/>
        </location>
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        <location>
            <begin position="2488"/>
            <end position="2497"/>
        </location>
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            <end position="2513"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2677"/>
            <end position="2694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2717" mass="310250" checksum="8BC8AEB9A5D544E6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKQ3</accession>
    <name>A0A1A0HKQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sphingolipid long chain base-responsive protein LSP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_10531</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018853793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKQ3"/>
    <dbReference type="GeneID" id="30026769"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="34204" checksum="249F318128C31573" modified="2016-10-05" version="1">MHRTYSMRSQKAPTASQMQSPPPPTSSTKSKFFGKGGLTSSFKKSFAGVSSPELSKKLTQYIKMEKNVMRAIELTARERRETAKQLSLWGEENDEDVSDVTDKLGVLIYEIGELEDQFIDKYDQYRITLKTIRNIEASVQPSRDRKQKITDEIAHLKYKDPQSPKISVLEQELVRAEAESLVAEAQLSNITREKLKAAFNYQFDAIRELAEKYALIAGYGKALLELLDESAVTPGETRAAYDGYEASKQIIIDAENALGSWTLDSAAVKPTLSLHQEDAEEPEEHELYEAEEEFAKHDETNGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HL33</accession>
    <name>A0A1A0HL33_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-terminal of MaoC-like dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_10654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714999.1">
        <property type="nucleotide sequence ID" value="XM_018853821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HL33"/>
    <dbReference type="STRING" id="869754.A0A1A0HL33"/>
    <dbReference type="GeneID" id="30026797"/>
    <dbReference type="OrthoDB" id="3257538at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019171">
        <property type="term" value="F:(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052741">
        <property type="entry name" value="Mitochondrial_HTD2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28152:SF1">
        <property type="entry name" value="HYDROXYACYL-THIOESTER DEHYDRATASE TYPE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="32147" checksum="A70FE1A9348BED06" modified="2016-10-05" version="1">MCRIKAQTRRWADIIAPRTWIWEDTYSIGGHNHLNKLLNEVISTRSQVSGPLYGSLFIFNTQSNTQLGLDGYDNYQAPLDVENNELYKRRMWVGGSIEYLKLPPVCGDVVVCEENVSSVRCLGPNAFVNISRNFRKDESMVMKEYRTLLYTNEPFRENASRFSETKGFLHSQNIKFSLSQLMRFNALSYNLHKIHYDAEFCHQEELKGIVVSGPFLVLAMLHFFSSLHPNTTIQDFRYKNSEPCYVDEGLIMGINETSRGYTIQVIGDGRLRCGGSVSIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GWJ9</accession>
    <name>A0A1A0GWJ9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_80228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000013">
        <property type="protein sequence ID" value="OBA16134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709240.1">
        <property type="nucleotide sequence ID" value="XM_018859196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GWJ9"/>
    <dbReference type="STRING" id="869754.A0A1A0GWJ9"/>
    <dbReference type="GeneID" id="30032172"/>
    <dbReference type="OrthoDB" id="3444765at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="83" mass="9205" checksum="1C6A1AE8113510E1" modified="2016-10-05" version="1">MPLDVLGRTRATLTEPARIPSKRKSSACVDYVPALCTHRPSLLPIEWLKTGQTWSFRGSKSRNKVSVGEPAEGSLKKNIIHSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ35</accession>
    <name>A0A1A0GZ35_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_80183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709313.1">
        <property type="nucleotide sequence ID" value="XM_018859189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ35"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ35"/>
    <dbReference type="GeneID" id="30032165"/>
    <dbReference type="OrthoDB" id="2441642at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013927">
        <property type="entry name" value="TF_Opi1_Ccg-8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38406:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR OPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08618">
        <property type="entry name" value="Opi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="397" mass="44104" checksum="747E551F7224D621" modified="2016-10-05" version="1">MPHPKQIKRKLSETSNAEPPRYADIVSPSWNSGSQQEPRDEDLVSAAEALTRLTNSATPPPSFHETHCTLALQTASPSSSTPVTEAGTQIHPIVKRVNTISKLPLVTNAVKYYETSKKNYATFNYAAGIVEKAAMPVFSKIEVNLNNKHQAKLEERARIKKKRRINKKHEKHEIKKRIKFCLHILKLANENISSKVVDLQEKMKETEALKTVKSESPTPVCQLTNESQTPGLSPEELTPISTNSPEKALETNNEIVATVKKIVHVISNFRPSSLNVGDDTNGGPNDDVKLKKTIREIILSLPKQVSQTVPGAQKAPHQAISFARESLDMIGRLTTVFNEQLEKAEHWVDRSTDDMSIDSLVSESPAPSELTKIDRPGVVRLKSEESFHSLYSQNDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H256</accession>
    <name>A0A1A0H256_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="14">dUTPase</shortName>
            <ecNumber evidence="5 14">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 14">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_13919</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:10249"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the dUTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="5 14"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709402.1">
        <property type="nucleotide sequence ID" value="XM_018854450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H256"/>
    <dbReference type="STRING" id="869754.A0A1A0H256"/>
    <dbReference type="GeneID" id="30027426"/>
    <dbReference type="OrthoDB" id="419889at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035863">
        <property type="term" value="P:dITP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.40.10:FF:000007">
        <property type="entry name" value="dUTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="15">
        <location>
            <begin position="21"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA18007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="153" mass="16117" checksum="BC8810F637DAB733" modified="2016-10-05" version="1">MSAEKKIKASDQLKVFLRSEKATVPTKGSALAAGYDLYASEDAVIPAQGQGMVATDVTIIVPIGTYGRVAPRSGLAVKHGISTGAGVIDADYRGEVKVVLFNHGKKDFEIKAGDRIAQLILEQIINADIQVITQEDLDATDRGEGGFGSTGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4R8</accession>
    <name>A0A1A0H4R8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UBC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_84741</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709480.1">
        <property type="nucleotide sequence ID" value="XM_018859240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4R8"/>
    <dbReference type="STRING" id="869754.A0A1A0H4R8"/>
    <dbReference type="GeneID" id="30032215"/>
    <dbReference type="OrthoDB" id="6508832at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031371">
        <property type="term" value="C:ubiquitin conjugating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010994">
        <property type="term" value="P:free ubiquitin chain polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044395">
        <property type="term" value="P:protein targeting to vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000026">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA18945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="137" mass="15295" checksum="FFB56DAEDE03FC36" modified="2016-10-05" version="1">MSKIPRNFKLLEELEKGEKGLGAQSISYGLTSQDDITMTNWNGTILGPPHSTHENRIYSLSIVCGKDYPEKPPTVTFVSKINLQCVDSKGNVVVSEFDTLKNWKRSNTMEIILLELRKTMASASNKKLSQPPEGSCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H544</accession>
    <name>A0A1A0H544_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL5m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33648</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709562.1">
        <property type="nucleotide sequence ID" value="XM_018856364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H544"/>
    <dbReference type="STRING" id="869754.A0A1A0H544"/>
    <dbReference type="GeneID" id="30029340"/>
    <dbReference type="OrthoDB" id="539541at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="5">
        <location>
            <begin position="187"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="290" mass="33311" checksum="F4CE2D31D293A450" modified="2016-10-05" version="1">MNKQWVRLFSSTGAKTKKYSTVEPVHHLVKVRKAALSPRYQPLLIPKDDIESTGFKPTEIDQDRLGEHYKNTLQSDLMLHFFDHANETIVGAKKRDWGTDSPYGLYRSWKKPSGTPRPTRDIKPITYKNVPELTGIAINLYSKEALEHAWLNISSKLQIAQITNVKPKQAYNKANVLQWKVREGRQCGCKVELTGRDMHQFFSTLTEMVLPRIRYYKGISNSSGDKGGNITFGLDPEHVKYFPEIENFQELFPNLFGFHITFKTSARTDEAARTLLSSMGIPFYTRPRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F7</accession>
    <name>A0A1A0H5F7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein-tyrosine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_79798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709803.1">
        <property type="nucleotide sequence ID" value="XM_018859129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F7"/>
    <dbReference type="GeneID" id="30032105"/>
    <dbReference type="OrthoDB" id="6375174at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052840">
        <property type="term" value="F:inositol diphosphate tetrakisphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000152">
        <property type="entry name" value="Tyrosine phosphatase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020428">
        <property type="entry name" value="PFA-DSPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004861">
        <property type="entry name" value="Siw14-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31126:SF74">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE-LIKE PROTEIN OCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03162">
        <property type="entry name" value="Y_phosphatase2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01911">
        <property type="entry name" value="PFDSPHPHTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="234" mass="26893" checksum="D1FBF4C810B089BE" modified="2016-10-05" version="1">MALNIDSSNATLWNCSHQRQNDPLNISSPRANYVPPLNFSLVEDEIYRSGFPMPINYPFLQQLKIKTIIYLGDLGEEKPKKKAKDDKKPKGGDEGTKEKKPKKDKHGTTEILENYKAWIATTDINFHHLVMHSSQEPFILNTQEGAQTALIGALSLMLDKRNFPILIHSNKGKHRIGVLVGLMRKIFQGWCMSGIFEEYEKFAMGKSEYDLEFIELWQPELAYEEAHLPEFVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5R6</accession>
    <name>A0A1A0H5R6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-nitrophenylphosphatase</fullName>
            <ecNumber evidence="3">3.1.3.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33546</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-nitrophenyl phosphate + H2O = 4-nitrophenol + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21664"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57917"/>
            <dbReference type="ChEBI" id="CHEBI:61146"/>
            <dbReference type="EC" id="3.1.3.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Divalent metal ions. Mg(2+) is the most effective.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.41" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709908.1">
        <property type="nucleotide sequence ID" value="XM_018856326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5R6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5R6"/>
    <dbReference type="GeneID" id="30029302"/>
    <dbReference type="OrthoDB" id="413953at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000039">
        <property type="entry name" value="Phosphoglycolate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006357">
        <property type="entry name" value="HAD-SF_hydro_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288">
        <property type="entry name" value="4-NITROPHENYLPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19288:SF46">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13344">
        <property type="entry name" value="Hydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000915">
        <property type="entry name" value="PGP-type_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00050247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00066659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00069197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000915-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="255" mass="28080" checksum="0F4E081166AD3B67" modified="2016-10-05" version="1">MAFVTNNSSKSRNTYVRKFSDLGFEHIEKSAIYPTCYSAALVIKEQLKIPPQSKVWVFGDEGIEQELKECGYIPCGGSDPSLDSEWDPDSPILKIDQEVKAVVVGSTKKLNYMRLATTVQYLLVDEKSIPFIGANIDTTYPGPKGMILPAGGPMVNLMSYTCGRDFINVGKPSGLLLNTILQDQKFDRERTLMVGDTMYTDIKFGNDGKLGGKNGGTLLVLSGGSKLGDLKNIEDKSTEPQYYIESLGHLFDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5S7</accession>
    <name>A0A1A0H5S7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_222245</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709918.1">
        <property type="nucleotide sequence ID" value="XM_018855153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5S7"/>
    <dbReference type="GeneID" id="30028129"/>
    <dbReference type="OrthoDB" id="415315at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990616">
        <property type="term" value="P:magnesium ion export from mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000095">
        <property type="entry name" value="Mitochondrial carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45667">
        <property type="entry name" value="S-ADENOSYLMETHIONINE MITOCHONDRIAL CARRIER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="45"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="140"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="246"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="363" mass="39194" checksum="083BB1E83C37C60F" modified="2016-10-05" version="1">MSQSYTTSAIPTPFQPHREAHHSTHNDPIGSTGAAASDDEIESASPLISCMLAGGFGGAIGDSAMHSLDTVKTRQQGLLFNPKYNSMIPAYTTILKEEGFLRGLYGGYSPAILGSFPSTAAFFGTYEFTKRTLINDLHINDTVAYLAAGILGDLALSVFYVPSEVLKTRLQLQGRHNNPYTKGCGYNYKGLRDAVRSIARTEGVSALTFGYKETLFRDLPFSALQLAFYEKFRLWAINYNGRSADLSVPAELLTGAAAGGLAGTLTTPLDVIKTRIQTATLDTAGLHQESTQHRTLGLGASILKRFSTLNALVAIHRNEGVLGLFSGVGPRFIWTGVQSSIMLLLYQVALKKLNGVFGEKVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6C6</accession>
    <name>A0A1A0H6C6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710109.1">
        <property type="nucleotide sequence ID" value="XM_018859095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6C6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6C6"/>
    <dbReference type="GeneID" id="30032071"/>
    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015233">
        <property type="term" value="F:pantothenate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098717">
        <property type="term" value="P:pantothenate import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000065">
        <property type="entry name" value="Putative MFS pantothenate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF4">
        <property type="entry name" value="PANTOTHENATE TRANSPORTER FEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="349"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="384"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="472" mass="54238" checksum="343FF511D399EB9A" modified="2016-10-05" version="1">MSKLKDVLLGKSSSDPAEVKLVRKIDWFVLSFCCLLYWVNYLDRLNLSNAYVSGMKEDLNMEGNQFNLINTCFNIGYIVALIPHNLILLKIRPRYWLSFCCVSWGLLTLSLYKATGYKFLCVVRFFQAMFEALTFTGCHLLLGSWYTEEELAKRAAIFTSSGLLGNIFSSTMQSAIYTNMDMKNGLAGWRWLFIIDFLITVPIAIYGLIFFPDTPDTCKAFYFKDEEIKLALQRVKPRPHTKLDWTVVKRVLGRWHWWMFSFLWVLGGENESFASNSLFSLWLQYFGYTVPQRNHYPMGVFAMGILANFVLCYYIDITHGKYHYRAGLVIAAFVLLSAILLTARPLDKSFVFASHYLSAWSFAGQPVFFSWANLVTYNDVQERAVVLASMNMMSNAVNAWWSLLFYAADTAPEFKKGCYAMIATAIASAVTVVFIRYLQLRDKREKPQDTILEDAVDGKSEDIVLEQTCEMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6D4</accession>
    <name>A0A1A0H6D4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33291</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710177.1">
        <property type="nucleotide sequence ID" value="XM_018856261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6D4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6D4"/>
    <dbReference type="GeneID" id="30029237"/>
    <dbReference type="OrthoDB" id="2020758at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="118"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="653"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="616"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="668"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="697" mass="78970" checksum="87F9B485F4F73D14" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6L7</accession>
    <name>A0A1A0H6L7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_216639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710080.1">
        <property type="nucleotide sequence ID" value="XM_018855091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6L7"/>
    <dbReference type="GeneID" id="30028067"/>
    <dbReference type="OrthoDB" id="4095887at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="688" mass="79473" checksum="8C079E9EBEECD818" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Q2</accession>
    <name>A0A1A0H6Q2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710115.1">
        <property type="nucleotide sequence ID" value="XM_018857996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Q2"/>
    <dbReference type="GeneID" id="30030972"/>
    <dbReference type="OrthoDB" id="4078936at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="249" mass="28827" checksum="A66E2400D60D82B9" modified="2016-10-05" version="1">MILLRFLRSLRTLSRVSFRHKGSFKNNDTTVETDEVVSDNNPWSPTLYQDKVYVRQGWKNVALPSKFRLSYEPLYESPGAKYVAILKRLTLSFAVLGVYGLKLLYELVQFDDIFALGVILGTWTPVAAVQYKTKDYVTRIFRLYDKEKPHTLENLVNDEQLIVEKLNATGGKTYNTLVTVLGNESLKLCTGEEDALKPYRTWQETVDGRKKFYYIMDNLGGMKMDRLWGIVEHNLGVDNGRYMEQKRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Q8</accession>
    <name>A0A1A0H6Q8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_206499</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710236.1">
        <property type="nucleotide sequence ID" value="XM_018854995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Q8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Q8"/>
    <dbReference type="GeneID" id="30027971"/>
    <dbReference type="OrthoDB" id="294295at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF12">
        <property type="entry name" value="OXIDOREDUCTASE, SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY (AFU_ORTHOLOGUE AFUA_1G14540)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="270" mass="28446" checksum="492F8C6BB6C7D244" modified="2016-10-05" version="1">MVDFNVNGKTAVVTGGTRGLGLYTAEAYVLGGAKTVVITSRNVKACQEAKAYLEKVAGENGKMTEIISIPADLAKEKECEQFFKSVMEKVATINILVANAGASWGAPLESHPVSAVKKILDLNVVAVFHTIQLFAPILEEAGTAEDPLRILIMSSVASLVASDPVGVYGYLASKSGVSHMGKNLALQLGPRNITVNSLAPGFFPTKMSQGVLDMAGDIMAELNPRKRLGVKEDIMSAVLFLSARQSNYINGIVLPIDGGSHVGGGFTPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6X5</accession>
    <name>A0A1A0H6X5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bola-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_79654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710186.1">
        <property type="nucleotide sequence ID" value="XM_018859106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6X5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6X5"/>
    <dbReference type="GeneID" id="30032082"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="76" mass="8772" checksum="923165AE2FEE3748" modified="2016-10-05" version="1">MEAYEQKIYDILHEKFTPKKLDVRDVSGGCGSMFAILVESEQFKGIPMIKQHRLVNEVLKDEIAKWHGLQLRTKGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H783</accession>
    <name>A0A1A0H783_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710288.1">
        <property type="nucleotide sequence ID" value="XM_018856139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H783"/>
    <dbReference type="STRING" id="869754.A0A1A0H783"/>
    <dbReference type="GeneID" id="30029115"/>
    <dbReference type="OrthoDB" id="2275718at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="34942" checksum="884B8EAB4BA61A60" modified="2016-10-05" version="1" fragment="single">MDDKNGAKADLVSSGAPSKAWGSANSPVSSGSAALSGALGAKKVGTKFKLNPRAAAFTPSRPPQMTPVPPKASPNKSDNSSPRTHGQRPYSNSSSSGSTGRRHYQVSPADFFGGVDKIPTAQSQKEKAKLFKNSFNMFAAAVKEHEGKSTALILEKAYKTPPTWDSTVDERYDKIVVKQSSGKVLPVSPVPHAPFMTSPVMAVPTGVPPMMAAGYMGSAGNGGKFPVSPHMQQPMGGQFQQQFHPSMMYSQFQGGVPPGQLQVMYAPPGMDPLLFSPGGFMMPGYMGSPVPMNGGASFQGVAHSHIKLARARATITVEDASIRTRGQEAMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7J5</accession>
    <name>A0A1A0H7J5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oleate-induced peroxisomal protein POX18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Lipid-transfer protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_208962</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Is involved in beta-oxidation of long-chain fatty acids. Its exact function is unknown, but possesses a nonspecific lipid-transfer activity, despite the absence of a cysteine residue thought to be essential for the activity of its mammalian counterparts.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710398.1">
        <property type="nucleotide sequence ID" value="XM_018855022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7J5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7J5"/>
    <dbReference type="GeneID" id="30027998"/>
    <dbReference type="OrthoDB" id="10265837at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000001">
        <property type="entry name" value="Putative Non-specific lipid-transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094:SF25">
        <property type="entry name" value="SCP2 STEROL-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10094">
        <property type="entry name" value="STEROL CARRIER PROTEIN 2 SCP-2 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP2" evidence="8">
        <location>
            <begin position="16"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00078512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="127" mass="13822" checksum="572A9EB02F7409EA" modified="2016-10-05" version="1">MSDLKADGFNASSLFQELNDGLKDEAAKKKAIAGVKAVIVITLKNKEGKDQSWVLNLKNEGSIKKTDSVPKNDVQLLMKDADFVKLASGKANGQKLFMNGKLKVKGNMMKATAIDSVFKSIRPKPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7P0</accession>
    <name>A0A1A0H7P0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">GAR transformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_79350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10-formyltetrahydrofolate = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GART family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710566.1">
        <property type="nucleotide sequence ID" value="XM_018859043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7P0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7P0"/>
    <dbReference type="GeneID" id="30032019"/>
    <dbReference type="OrthoDB" id="5575075at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000009">
        <property type="entry name" value="Phosphoribosylglycinamide formyltransferase (Eurofung)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43369:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl transferase N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="235" mass="25711" checksum="2DE23B999C12A205" modified="2016-10-05" version="1">MEITVLISGSGSNLQALLEAQKNSLLKGKITQVISSSEDAYGLQRAAKFSVPSKTHVLKKYYKGTTKDDKEVRREKREQFNKDLANLLIHGNIEGTKDSTYSKPGLIVCAGWMLILSPSVLTPLAKAGISIINLHPALPGAFEGTHAIDRAWKAGQDGEITKGGVMIHKVIAEVDRGEPVLVKELDFIKGETLEEYEGRVHKVEHEAIVEGTNIVLEELSADELVEKCEKLLVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7R0</accession>
    <name>A0A1A0H7R0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HAD-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710586.1">
        <property type="nucleotide sequence ID" value="XM_018859049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7R0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7R0"/>
    <dbReference type="GeneID" id="30032025"/>
    <dbReference type="OrthoDB" id="10014216at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110130">
        <property type="term" value="F:ribitol-5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000121">
        <property type="term" value="F:sn-glycerol 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043136">
        <property type="term" value="F:sn-glycerol 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050286">
        <property type="term" value="F:sorbitol-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052646">
        <property type="term" value="P:alditol phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050849">
        <property type="entry name" value="HAD-like_hydrolase_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006384">
        <property type="entry name" value="HAD_hydro_PyrdxlP_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01489">
        <property type="entry name" value="DKMTPPase-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181:SF2">
        <property type="entry name" value="PHOSPHORIC MONOESTER HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181">
        <property type="entry name" value="UPF0655 PROTEIN YCR015C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="27295" checksum="7768C4A66882FBCA" modified="2016-10-05" version="1">MSLPAIIFTDWDGTVTLQDSNDYLTENLGFGRPRRLEINDEILNESKSFKDGFSEMLSSIPTPFPECIEYLLNNIQLDPGFKDFYTWCESKGIPVIVVSSGMTPIIHALLQSLVGEEAVKNIQIISNDVDIDPATGKWKIVYRDPDTGFGHDKSRSIREYLKANGYTAENMPPLFYCGDGVSDLSAAKETNLLFAKHGKDLITYCRRENIPYTEFNDFADIFSKIRSIVEGGKRYEDYIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7S6</accession>
    <name>A0A1A0H7S6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cytochrome c oxidase, subunit VIb</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_211731</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase subunit 6B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710560.1">
        <property type="nucleotide sequence ID" value="XM_018855043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7S6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7S6"/>
    <dbReference type="GeneID" id="30028019"/>
    <dbReference type="OrthoDB" id="5545577at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:intracellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.140">
        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048281">
        <property type="entry name" value="COA6_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048280">
        <property type="entry name" value="COX6B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47677:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02297">
        <property type="entry name" value="COX6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47694">
        <property type="entry name" value="Cytochrome c oxidase subunit h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="12315" checksum="292E4F6822E8CA8E" modified="2016-10-05" version="1">MGLFTKDVAVVDPPNKTKRKICWDSRDKFFDCLESNKIENSLDPKKSEQVESSCGGERAEFQKNCVASWFKYFQEKRYNDIKRQKYIAQLEAEGAKPLPFKLDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U3</accession>
    <name>A0A1A0H7U3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="6">HMG-CoA synthase</shortName>
            <ecNumber evidence="6">2.3.3.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-hydroxy-3-methylglutaryl coenzyme A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_79283</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710487.1">
        <property type="nucleotide sequence ID" value="XM_018859031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7U3"/>
    <dbReference type="GeneID" id="30032007"/>
    <dbReference type="OrthoDB" id="1269963at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000008">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl coenzyme A synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000590">
        <property type="entry name" value="HMG_CoA_synt_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010122">
        <property type="entry name" value="HMG_CoA_synthase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01833">
        <property type="entry name" value="HMG-CoA-S_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01226">
        <property type="entry name" value="HMG_COA_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="8">
        <location>
            <begin position="174"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00073381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR610122-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="447" mass="49370" checksum="E112E87689EC8BFB" modified="2016-10-05" version="1">MAPQNVGIKAIEVYIPTNYVNQSDLETFDGIPAGKYTIGLGQTNMAFVSDREDIYSLSLTVTSRLLKNYNIDPSSIGRLEVGTETLLDKSKSVKSVLMQLFQDNDDIEGVDTVNACYGGTSAVINAVNWVESSSWDGRDAIVVAGDIAIYSQGAARPTGGVGAVALLIGPDAPVVFDSARGSHMEHAYDFYKPDFTSEYPVVDGHFSLTCYTKSVDRAYKNYSKKVTKDTNKTVGFLDYFDYSAFHVPTCKLVTKSFARLLYNDFLSNPEAFRGKLDQDTFDQVSAVSYEQSLTDRNIEKVFLGLAKEESNKRLASALKVPTNTGNMYTASCWASLASVLYFAGNEELQNKRISIFSYGSGLASTLLSVRVVGDISAITKVLNLDYKLGEGRQKKTPEEYVEHIALREKAHLQKNFKPQGSVEDIAPGAYYLVNIDDKFQRTYEIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Y6</accession>
    <name>A0A1A0H7Y6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Mitochondrial cytochrome c oxidase assembly factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_79425</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PET100 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710661.1">
        <property type="nucleotide sequence ID" value="XM_018859063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Y6"/>
    <dbReference type="GeneID" id="30032039"/>
    <dbReference type="OrthoDB" id="18175at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018625">
        <property type="entry name" value="Pet100"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33968:SF1">
        <property type="entry name" value="PROTEIN PET100 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09803">
        <property type="entry name" value="Pet100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11432" checksum="C17AB53014911D06" modified="2016-10-05" version="1">MEFYQKFIGNITRTQLETFKFGFYLLTPILVMYYVGIDTDKKFNLPGFWPDPVTLNQIPKEPHEIQAEVARIRRARLEKRERLEAKARELGILEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H802</accession>
    <name>A0A1A0H802_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32253</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for pre-18S rRNA processing. May bind microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710751.1">
        <property type="nucleotide sequence ID" value="XM_018856016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H802"/>
    <dbReference type="STRING" id="869754.A0A1A0H802"/>
    <dbReference type="GeneID" id="30028992"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902570">
        <property type="term" value="P:protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000003">
        <property type="entry name" value="Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000001">
        <property type="entry name" value="Probable Nucleolar protein 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Nop" evidence="10">
        <location>
            <begin position="283"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="432"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="432"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="444"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="471"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="496"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="521" mass="57598" checksum="210480634EF29938" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H889</accession>
    <name>A0A1A0H889_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_43956</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710717.1">
        <property type="nucleotide sequence ID" value="XM_018857725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H889"/>
    <dbReference type="STRING" id="869754.A0A1A0H889"/>
    <dbReference type="GeneID" id="30030701"/>
    <dbReference type="OrthoDB" id="1747252at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_uL1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291688" description="Ribosomal protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="277" mass="30543" checksum="6DD62CD23F745A8B" modified="2016-10-05" version="1">MFAQPLKRLAAAPLIRLFSLYAFASQEIAIRHPSPKLTATKDPKKIAAREAKLRARQQVKPQDHLLYMDIPKAMRFMRASEVGKSPEMTTITLQITVIPEKGSHPLLGQIIFPKPVRPNNTLIFTENAEQIQEIKRSKELTLVGGADLIAKIKEGFPVASYTQCLADSNMVLQLGQIAKILGPKNLMPSAKKGTVSDNVVDLLKNTASAYTFKQKKNQLYFPVGRCDFSDEEIIKNVQAASKAVYSCQPPGTKHPNLIGLCSMSSTLGPSVIIDFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8H1</accession>
    <name>A0A1A0H8H1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Dihydrolipoyl dehydrogenase</fullName>
            <ecNumber evidence="11">1.8.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_196167</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + NAD(+) = N(6)-[(R)-lipoyl]-L-lysyl-[protein] + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15045"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="1.8.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9 11">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710843.1">
        <property type="nucleotide sequence ID" value="XM_018854875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8H1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8H1"/>
    <dbReference type="GeneID" id="30027851"/>
    <dbReference type="OrthoDB" id="361797at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004375">
        <property type="term" value="F:glycine dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006546">
        <property type="term" value="P:glycine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042743">
        <property type="term" value="P:hydrogen peroxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006550">
        <property type="term" value="P:L-isoleucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006552">
        <property type="term" value="P:L-leucine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.30:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000001">
        <property type="entry name" value="Dihydrolipoyl dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050151">
        <property type="entry name" value="Class-I_Pyr_Nuc-Dis_Oxidored"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006258">
        <property type="entry name" value="Lipoamide_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01350">
        <property type="entry name" value="lipoamide_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912:SF151">
        <property type="entry name" value="DIHYDROLIPOYL DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22912">
        <property type="entry name" value="DISULFIDE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9 11" id="KW-0274">FAD</keyword>
    <keyword evidence="2 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5 9 11" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="13">
        <location>
            <begin position="20"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="12">
        <location>
            <begin position="358"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="198"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="330"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="10">
        <location>
            <begin position="58"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="477" mass="51222" checksum="B88A06F41C3E329E" modified="2016-10-05" version="1">MLRSNIRQFVRFNSSKAGHYDAVVIGGGPGGYVHAIKLAQLNYKTACIESRGSLGGTCLNVGCIPSKALLNNSHLYHQIKHDSANRGIEIAGDVSVNVSKLQAAKDKAVKGLTSGVEMLFKKNGVTYLKGYGSFVDDKTVSVKGLDGDEYKVTADNITIATGSEPTPFPGIEIDEERIVSSTGALTLKEVPKKMAIIGGGIIGLEMASVWSRLGSEVTIIEFQDSIGAGMDAEVAKTTQKLLAKQGLKFKLNTKVTKGVREGENVKSGKTEEMDADVLLVAIGRRPFTKGLNHEVLGLEQDDKGRLVIDSQFRTAKPHIRVIGDVTFGPMLAHKAEEEGIAAAEYIKHGHGHVNYGNIPSVMYTHPEVAWVGANEQQLKADGIKYKVGKFPFIANSRAKTNLDTDGFVKFLADAETQRVLGVHIIGPNAGEMIAEGGLAMEYGASTEDIARTCHAHPTLSEAFKEAALATFDKPINF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8J2</accession>
    <name>A0A1A0H8J2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710832.1">
        <property type="nucleotide sequence ID" value="XM_018857763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8J2"/>
    <dbReference type="GeneID" id="30030739"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291693" description="Secreted protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7247" checksum="1980EB5962904C15" modified="2016-10-05" version="1">MIPKKIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPKVHSVCQIRSIELLYCFASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8K3</accession>
    <name>A0A1A0H8K3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_23699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710735.1">
        <property type="nucleotide sequence ID" value="XM_018855266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8K3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8K3"/>
    <dbReference type="GeneID" id="30028242"/>
    <dbReference type="OrthoDB" id="331341at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039905">
        <property type="entry name" value="CD2BP2/Lin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003169">
        <property type="entry name" value="GYF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138:SF3">
        <property type="entry name" value="CD2 ANTIGEN CYTOPLASMIC TAIL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138">
        <property type="entry name" value="PROTEIN LIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02213">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00444">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50829">
        <property type="entry name" value="GYF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GYF" evidence="2">
        <location>
            <begin position="256"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="35587" checksum="720FC13610A6D684" modified="2016-10-05" version="1" fragment="single">ENGNDKEDDMFASDKEESEPQRTEPKGRAVIDIAQFEKDQGLEDYEFNAGMDKDEIKPHLLHLEVQLEAFNLREEAESGNFDKDMNYIQHDKSDSEDDEPWMAGVSVADIEKARKAQQKSSRAGSQKYSVSSKSVPEILPDLILVLEPAETPMEALSRLRPEKKKLKQKTADLNETERKNAVFKLTDCCEQLVNDKGMTGAYDMSREELMRAYFQETGVNFQPRGVKRDVEQMEKDDGQHGTIENQHATGENQYGKAIWEFRWVDDTEVHGPYSSYEMDHWKKTYFENRVEVRRIGDPVFCPVAEVLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8P6</accession>
    <name>A0A1A0H8P6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_78700</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710779.1">
        <property type="nucleotide sequence ID" value="XM_018858923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8P6"/>
    <dbReference type="STRING" id="869754.A0A1A0H8P6"/>
    <dbReference type="GeneID" id="30031899"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009927">
        <property type="term" value="F:histidine phosphotransfer kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036180">
        <property type="term" value="P:filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007234">
        <property type="term" value="P:osmosensory signaling via phosphorelay pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900445">
        <property type="term" value="P:positive regulation of filamentous growth of a population of unicellular organisms in response to biotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.130:FF:000004">
        <property type="entry name" value="Ethylene receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000289">
        <property type="entry name" value="Osmosensing histidine protein kinase SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047:SF72">
        <property type="entry name" value="OSMOSENSING HISTIDINE PROTEIN KINASE SLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43047">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="19">
        <location>
            <begin position="472"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="17">
        <location>
            <begin position="539"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="18">
        <location>
            <begin position="1035"/>
            <end position="1156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="403"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="707"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="780"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="967"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="735"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="790"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="969"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="980"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="14">
        <location>
            <position position="1090"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA20257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1193" mass="132479" checksum="BAECA0F974585745" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8S3</accession>
    <name>A0A1A0H8S3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein bL27m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">54S ribosomal protein L2, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_57681</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711041.1">
        <property type="nucleotide sequence ID" value="XM_018858300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8S3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8S3"/>
    <dbReference type="GeneID" id="30031276"/>
    <dbReference type="OrthoDB" id="1867012at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000042">
        <property type="entry name" value="50S ribosomal protein L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_bL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_bL27_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041244">
        <property type="entry name" value="Ribosomal_bL27m_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL27M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18471">
        <property type="entry name" value="Ribosomal_L27_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein bL27m C-terminal" evidence="10">
        <location>
            <begin position="136"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="376" mass="42152" checksum="413D8D852211F58A" modified="2016-10-05" version="1">MSFLSQGLIAQVGTSIKAPSGALVHIRTATKKVSGSKTNKNDSAGRRLGPKAYENHFVKTGQIIMRQRGTKIHPGENVGLGRDHTIFALEPGYVRFYLDPFHPLRKYVGVALRKDIQLPKPHFDPRIRRFGYKPLDPKRAEAEMARMSRKELLAQPELKKRAEEFAKETSLKISAYSACIAELLPYTGENDQISGATRLLFIARKLALNETYAYASEQATFNVVHELVLLNKRGEISKEELNEKTASYIAFAKDFDSKISVDFTGAVHPYYNDESRAAKAHEIREKLKEFKEKLIGPTEKSQISDLISEAGIFDKEERQTLTSQYLPKVLPESVPGTVIEGVSSKKIPKNAVAVRTFDSKNRTVATVLRTKDAFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U1</accession>
    <name>A0A1A0H8U1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_32317</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion inner membrane</location>
            <topology evidence="1 13">Peripheral membrane protein</topology>
            <orientation evidence="1 13">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small Tim family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710825.1">
        <property type="nucleotide sequence ID" value="XM_018856027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8U1"/>
    <dbReference type="GeneID" id="30029003"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140318">
        <property type="term" value="F:protein transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000008">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit TIM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 13" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="14">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA20303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="83" mass="9944" checksum="304786B0B3E90777" modified="2016-10-05" version="1">MDQLNVKEQQEFQQVVEQKQMKDFMRLYSNLVSRCFDDCVNDFTSANLTSKESSCIFKCSEKFLKHSERVGQRFQEQKYVDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H911</accession>
    <name>A0A1A0H911_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_43498</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711012.1">
        <property type="nucleotide sequence ID" value="XM_018857665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H911"/>
    <dbReference type="STRING" id="869754.A0A1A0H911"/>
    <dbReference type="GeneID" id="30030641"/>
    <dbReference type="OrthoDB" id="1701437at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025654">
        <property type="entry name" value="PEX2/10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23350:SF4">
        <property type="entry name" value="PEROXISOME BIOGENESIS FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="264"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="348"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="348"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="398"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA20490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="48229" checksum="4B58134C39617DF9" modified="2016-10-05" version="1">MPVPRISYPSHRVSQLDASILDDELFSLLKQQLSDSFLLISSQPWSYNLHPELWSLILKLVIFKLTTLKTGSSYGFKLQNLKLSQKDSGRVIGNHMRYILLATILGEYIVKKVQSYIFSLEKLSYRPQTLIERVKNALYPYKTNIIKASGDTIQVLELLNFVSFLVFGRYPNLIYRALGITVTPIVADLLKFNGSNVNFEFQNRQLIWNVMTEFLVFILPLLQFRKLMRMLRSFLPKKGNGHRFHGYSESPITTNFTSLPISQCAMCIETTEARGIKAASTVITNPCVTNCNHIFCYICLATRLNAIENGNERAEGCPRCRLRITSFKQYGVELGNVDKSAIMVEYDEAGSEGSEAEEDYQDAVQLNTSADYLDAHTEQSSESEQPFESHSPDYSEHEDLEEEGEYSDDFDSGAKDDWEDGVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H941</accession>
    <name>A0A1A0H941_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein 60S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_88020</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711048.1">
        <property type="nucleotide sequence ID" value="XM_018859285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H941"/>
    <dbReference type="STRING" id="869754.A0A1A0H941"/>
    <dbReference type="GeneID" id="30032260"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF33">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="106" mass="10507" checksum="0CC5BE92A9715F05" modified="2016-10-05" version="1">MSAEASVSYAALILADAGVEISSDNLLSVTKAAGASVDNVWADVFAKALEGKDLKELLFSFAASAPAASSGSAAAAAGGATEAAAEEKVEEAAEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H987</accession>
    <name>A0A1A0H987_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">low-specificity L-threonine aldolase</fullName>
            <ecNumber evidence="8">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_43507</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid degradation; L-threonine degradation via aldolase pathway; acetaldehyde and glycine from L-threonine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710960.1">
        <property type="nucleotide sequence ID" value="XM_018857666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H987"/>
    <dbReference type="STRING" id="869754.A0A1A0H987"/>
    <dbReference type="GeneID" id="30030642"/>
    <dbReference type="OrthoDB" id="10261951at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000030">
        <property type="entry name" value="Low-specificity L-threonine aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023603">
        <property type="entry name" value="Low_specificity_L-TA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041359">
        <property type="entry name" value="GntG_guanitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097:SF9">
        <property type="entry name" value="L-THREONINE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48097">
        <property type="entry name" value="L-THREONINE ALDOLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017617">
        <property type="entry name" value="Thr_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aromatic amino acid beta-eliminating lyase/threonine aldolase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00050410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR017617-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="383" mass="42113" checksum="604029E89650AC13" modified="2016-10-05" version="1">MTVPAFEYSTHNEFRSDTFTVPTASMNNAVVQGLSDGSLLVGDSVYKEDPLTLQLEAKMSALTGKPAALFCASGTLSNQVALRAHLHQPPYSILCDHRAHVFLHEAGGLASLSQAMVHPVIPQNGNYLTLEDIEDNFTPSDGDIHGAPTKVISLENTLHGIITPIGEIRRISQFARENNLRLHLDGARLFNALVETGVSLKEYCSYFDSVSICLSKSVGAPMGSVLVGEQPLIDTANHFKKQCGGGIRQVGIMALMAMAAIDENVSLIHRSHEYVKEVAQFCAEHDIALESPPDSNFIFLDMKKNKMDDKKLVAFGNKHHVRLMGKRIAFHFQLSRESVHNLKQALLESKQDAIQNPYVGRSLAKNMYNVDVVKLIQEMALHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9A1</accession>
    <name>A0A1A0H9A1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710975.1">
        <property type="nucleotide sequence ID" value="XM_018857697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9A1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9A1"/>
    <dbReference type="GeneID" id="30030673"/>
    <dbReference type="OrthoDB" id="531564at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="52" mass="6148" checksum="F0DBD315B5F16507" modified="2016-10-05" version="1">MAAHHIAKYVRHAAPAKPHVDPRLKWSSKFLGAAMWFYIFYRVKNDFIEGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q1</accession>
    <name>A0A1A0H9Q1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711134.1">
        <property type="nucleotide sequence ID" value="XM_018856093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q1"/>
    <dbReference type="GeneID" id="30029069"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="142" mass="15782" checksum="43E67E37910BD98F" modified="2016-10-05" version="1">MASFFWGFLVCFGVFWCVLVWWYFGAGWGCFALVSGVLVSWCWCLVVVFWLLVFGVLVFGVLVFGVLVFGVLVFGVLVFGVLVFGVLAFDGIVFDGGDVLLSLFEKNARYGEARLMIRAPTRKAEFAYRSGASGRWTRDVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W8</accession>
    <name>A0A1A0H9W8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_205899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711317.1">
        <property type="nucleotide sequence ID" value="XM_018854985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W8"/>
    <dbReference type="GeneID" id="30027961"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="178" mass="18851" checksum="C6FDE22FEC5AA254" modified="2016-10-05" version="1">MWPRASAPVTPRSLLSLHSGFLTGPAVFSFRSSPSCLGTRAATAAALLTGPACSFIFACVPAPQARPQKPRPASSLTATPSPTRFSFPAARLPAAQHPSYFPCGFSLLLFFCSLPPAALALASLAICAPTIGRPSAPVPLPPSHTYPAPGFYFFFPRPARKLSTRFARQISTEPRTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA13</accession>
    <name>A0A1A0HA13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_32662</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711235.1">
        <property type="nucleotide sequence ID" value="XM_018856109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA13"/>
    <dbReference type="STRING" id="869754.A0A1A0HA13"/>
    <dbReference type="GeneID" id="30029085"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000003">
        <property type="entry name" value="60S ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018256">
        <property type="entry name" value="Ribosomal_eL13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01104">
        <property type="entry name" value="RIBOSOMAL_L13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="198" mass="22423" checksum="2CEFF85A0984B72D" modified="2016-10-05" version="1">MAISKNQPLLKNHFRKHWQERVRVHFDQAGKKNSRRQARLQKAAKVAPAPVDALRPIVRCPTIKYNRKVRAGRGFTLAEIKAAGLTAKYARSVGIAVDHRRQNKSQENFDANVARLNEYKSKLVIFDKKSKAEGEQISTAAAFPIKQASTETTTAAVEVPAQSAYTTLRVARNEKKYWGIREKRAREKAEAEAEKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA34</accession>
    <name>A0A1A0HA34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_11486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711386.1">
        <property type="nucleotide sequence ID" value="XM_018854002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA34"/>
    <dbReference type="STRING" id="869754.A0A1A0HA34"/>
    <dbReference type="GeneID" id="30026978"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291708" evidence="2">
        <location>
            <begin position="21"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="131" mass="14830" checksum="5A52D6AA9C88C343" modified="2016-10-05" version="1">MVVLNVLLVIKWLEITLSKAFVEYLLSESVGSHFLEAIIKNDGARMKYIERLHKLSMKARVLKLAKRATTGACINKASMVKLKPGEISGLVSVSENQNIELCKSVIEASMSRFNYRRDNLVQQLFKKFALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA99</accession>
    <name>A0A1A0HA99_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">General transcription and DNA repair factor IIH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_44351</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GTF2H2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711324.1">
        <property type="nucleotide sequence ID" value="XM_018857781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA99"/>
    <dbReference type="STRING" id="869754.A0A1A0HA99"/>
    <dbReference type="GeneID" id="30030757"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000015">
        <property type="entry name" value="General transcription factor IIH subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004595">
        <property type="entry name" value="TFIIH_C1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012170">
        <property type="entry name" value="TFIIH_SSL1/p44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00622">
        <property type="entry name" value="ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07975">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015919">
        <property type="entry name" value="TFIIH_SSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01047">
        <property type="entry name" value="C1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="12">
        <location>
            <begin position="364"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR015919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR015919-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="473" mass="51050" checksum="B2901A3DDAA44168" modified="2016-10-05" version="1">MARRTRLKAPTSTLKSASGAYAWEDQYQRPWDLVAADGDAGASLELLVQQAIERRRNKILKHPSAPFQRGILRTLVVVVDGLRAMLDKDLRPTRLACTLALLQDFVREFFDQNPVAQMAVVWMRNGVASVVLALSGLPQQHIDRLRAVRARQHNRYEPQGDPLLQNALELARLLLVLALGAAPGVAPPAQKASREILVVFGALFTLDPGDIHGTITALAREHIRAKVIGLLAQVAVCQELVRRTNLPHAGRPGLRADARLARFYGVIINEHHFRELLMDCVEPLAVPRGAGPAVPGDALGVPVIRMGFPLRLPAPALPAANLPRLCACHPTRGSAALREPVPVQPVPPPGTSAAVPDPPAAYTCPQCRSRVCSVPTVCPACGLMLILSTHLARLYHHLVPLAAYTTVAAAPAYPSSHCYGCLLEFPPGSPEAPGPAASSLRFRCGKCAHDFCIDCDVFVHEVLHNCPGCENRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAF1</accession>
    <name>A0A1A0HAF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_170482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711501.1">
        <property type="nucleotide sequence ID" value="XM_018854686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAF1"/>
    <dbReference type="GeneID" id="30027662"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="100" mass="11144" checksum="291477F7BF2A4105" modified="2016-10-05" version="1">MCKTRDIVQCSRGRWGLTNKQLVQAKLARGSDANTKEQGGRAAIASGWTREIFGFFLGFFGGFEGSWYMWVKVSSKLKRKLNCATANLDEQATGDEMTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAI6</accession>
    <name>A0A1A0HAI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">pH-response regulator protein palF/RIM8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_19789</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the arrestin family. PalF/RIM8 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711525.1">
        <property type="nucleotide sequence ID" value="XM_018854894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAI6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAI6"/>
    <dbReference type="GeneID" id="30027870"/>
    <dbReference type="OrthoDB" id="7785529at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070086">
        <property type="term" value="P:ubiquitin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF161">
        <property type="entry name" value="PH-RESPONSE REGULATOR PROTEIN PALF_RIM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="4">
        <location>
            <begin position="275"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="479"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="487"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="550"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="578"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="578" mass="63270" checksum="A2420A8FD0A76850" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAK2</accession>
    <name>A0A1A0HAK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component SEC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41326</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the exocyst complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SEC5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711669.1">
        <property type="nucleotide sequence ID" value="XM_018857384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAK2"/>
    <dbReference type="STRING" id="869754.A0A1A0HAK2"/>
    <dbReference type="GeneID" id="30030360"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029175">
        <property type="entry name" value="EXOC2/Sec5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13043">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="5">
        <location>
            <begin position="75"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="966" mass="111415" checksum="8DE2044B7464FEE0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ9</accession>
    <name>A0A1A0HAQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_11563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711477.1">
        <property type="nucleotide sequence ID" value="XM_018854018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAQ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAQ9"/>
    <dbReference type="GeneID" id="30026994"/>
    <dbReference type="OrthoDB" id="5600212at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052783">
        <property type="entry name" value="Metabolic/Drug-Res_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655">
        <property type="entry name" value="QUINIC ACID UTILIZATION ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47655:SF3">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="457" mass="49110" checksum="483E1701828C0E22" modified="2016-10-05" version="1">MSVSSPDEQHHHRKKRVGKACDLCRIKKTKCDGKKPCTRCVADNQLCAFSEKRKPKEKAHPAGYIELLEARIDFLTRSLQKVITMAEPHLPFLQQIMEQARLEHSQSAPLCSDDSPLPSAIPINEVVNFLISDPDFMPGQLPETMHRADAAANTPSAKLAPGPAIKDEFPGNSPRNEVLFLDPTSDGVDNLGQQYDLVSALNSGTPAAHLMRLPGGSSALDSRSLFHRHGSLPPSRSSSSHLLLSSANEPFSGLPASSASLISGRGLHHEAVPNGGAPLNSPPPETLASELASLDSIDHFSQYALRSSLLFLNNGGTVCTSPLSVSSLSIKLESQNFNDLARHSLRRLHSGKAHVSAASIKKKNSGHVHKPQSVSHSPQIGSANILPSAASRQEPGPAFPALVYPYLQTALPDLGLFGFENDHLFDEPPSKVIADGLPSFLALDDLGMTNPFMSPYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAR0</accession>
    <name>A0A1A0HAR0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_42022</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711590.1">
        <property type="nucleotide sequence ID" value="XM_018857481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAR0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAR0"/>
    <dbReference type="GeneID" id="30030457"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="201" mass="22883" checksum="F95BF8A7B62B2A9C" modified="2016-10-05" version="1">MLSLYLKTYNVLSALAWAVILFKDIIDRIPGQLYHVGYSAFPHKLLTEVQTANAIFEIAHALVGIVPSPLGSLLLQFFARLVITLGISYYVPASPGNYSMAYSALVAAWSITEIIRYSFYAAKQNRHVPRVLLWLRYLSFIVLYPLGLLSEPVVVYKTLGHVSGGYYYFLALGMLMYVPGFVFLYLYMWKQRKKYLVAKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAX4</accession>
    <name>A0A1A0HAX4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_82809</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711655.1">
        <property type="nucleotide sequence ID" value="XM_018859222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAX4"/>
    <dbReference type="STRING" id="869754.A0A1A0HAX4"/>
    <dbReference type="GeneID" id="30032197"/>
    <dbReference type="OrthoDB" id="339151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990861">
        <property type="term" value="C:Ubp3-Bre5 deubiquitination complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039539">
        <property type="entry name" value="Ras_GTPase_bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693:SF20">
        <property type="entry name" value="AT27578P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10693">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="NTF2" evidence="5">
        <location>
            <begin position="43"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="409"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="224"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="198"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="231"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="243"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="292"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="501" mass="54273" checksum="B9950A58E39B19BD" modified="2016-10-05" version="1">MLQATKPPAEPAATKPEPPKPEPVKSVAPAEAVPQPDLSSEQIGWMFLKKYYGFYTADIRKLYAFYDSAAALLHDVFPGNGRKTVHVARGSDAVRSFFDGQAAQASAADKNKIVVESATFQASVAGAILIVVAGGWKRGVSPFWPFVQTFVLREKSKTVYDVANDVLTFVDLAGEPAEAAAAVAERVAEPSAKPVQASEPQTADQNGSSEPAACTALLQKEAVLETRSEEPVPETTEESSPETSKEPAPEAKEKAAPETKEKSAPETKEQPASPEDASSSAAETAASAETVKPADQEKPDQKQTDQEKPGQEKTDLEKPGQEKTDQEKPDQEQSAEPEKPSVPPQKPTWANLAAIGPKTNAKTAAVASPAAIKIPPPPVPAFAAKKAASPSQAQGPSPVNGKYKKEEWYPIFIRNVDVDEDELKAALVENFGEMKYFKKTQKTALCDFRRKEDQQKALEQKEMVIKSNVISLEVRVHKAYNGKPDPKKDKKHLKKIPFKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB97</accession>
    <name>A0A1A0HB97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_42245</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711914.1">
        <property type="nucleotide sequence ID" value="XM_018857517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB97"/>
    <dbReference type="STRING" id="869754.A0A1A0HB97"/>
    <dbReference type="GeneID" id="30030493"/>
    <dbReference type="OrthoDB" id="336885at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016233">
        <property type="term" value="P:telomere capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054300">
        <property type="entry name" value="OB_DPOA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22062">
        <property type="entry name" value="OB_DPOA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018300">
        <property type="entry name" value="DNA_pol_alph_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha subunit B OB" evidence="9">
        <location>
            <begin position="212"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="8">
        <location>
            <begin position="348"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="666" mass="72710" checksum="643D5839F8039185" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBA1</accession>
    <name>A0A1A0HBA1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711678.1">
        <property type="nucleotide sequence ID" value="XM_018857511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBA1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBA1"/>
    <dbReference type="GeneID" id="30030487"/>
    <dbReference type="OrthoDB" id="4023585at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="178" mass="19149" checksum="93E39C9C489C14AD" modified="2016-10-05" version="1" fragment="single">ISSNMFRQIASRTIAPLSARVSPNARYLSYSRVNLSVVDTAKDILHKANKKTGEVLANTIDTTEKVTPSGEQVKDAATNAASAANQKAGDVLADGIEKAEDISHKAKQTATDANLGEKAKDAAQSAQQFSHKAKETTIDMKDHANAKKKVLENARGYENLQDKGSKVETEQNRPDDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBD8</accession>
    <name>A0A1A0HBD8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">INO80 complex subunit B-like conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711959.1">
        <property type="nucleotide sequence ID" value="XM_018858878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBD8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBD8"/>
    <dbReference type="GeneID" id="30031854"/>
    <dbReference type="OrthoDB" id="2021186at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029523">
        <property type="entry name" value="INO80B/Ies2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006880">
        <property type="entry name" value="INO80B_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21561:SF12">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04795">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01406">
        <property type="entry name" value="PAPA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INO80 complex subunit B-like conserved region" evidence="2">
        <location>
            <begin position="189"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="271" mass="31541" checksum="7CA05F25FF20FF6F" modified="2016-10-05" version="1">MSARRSRQKIAYSDDDDFDAAPPQNASDSDDASEPEFDEDPEDFDDFEDEDHQPQEAEDSAEEAYEAEPKVSARPLRGGRPVRETKKRQLTFYEEDDLLDSDEEAPAPKKRVSVKLKIPRRAAPKKEEDFLEPEPEYRPDVLRMTERQRARLAEDEPNEKYEELMFARMDEQLLALNRKTQKKKETAEEIAVRKAENARKRADYKIKQLEEEKRDTLNKLLKRRANKTREKEDDDESDSKQTLKPRRPVVGHAALMRWVSLSAGSVLAFTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBJ1</accession>
    <name>A0A1A0HBJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="14">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 14">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_31815</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the TFB4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711763.1">
        <property type="nucleotide sequence ID" value="XM_018855926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBJ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBJ1"/>
    <dbReference type="GeneID" id="30028902"/>
    <dbReference type="OrthoDB" id="17307at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 14" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="355"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="361"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="394" mass="42732" checksum="8A10E42A55E0DEF5" modified="2016-10-05" version="1">MDKISESAFAELPVTEDVSENCSLLTVILDLSPTAWHAIRAQVSIQETVKSLLVFLNAHLALNNSNQVAVIASLPFGSRFLHPSTGRRYEDVDENSEGGSEKPKATLINEGMYRQFRLVDTAVLLELNEELRRLATPGSGDLKFLNVSTLSGALLMALTYTNRMLHFDQSILTTTASAINSTTSAVNSTGSAEPSVHAGSVLPTSLDSRILIVSASDSHDTHYIAIMNSIFAAQKMKVPIDVAKLGRLDSPYLQQAADATKGVYLHITEPKGLIQTLSTAYFIEPCLRSVVILPTNSNVDYKASCFILGKPVDIGYVCSVCLCIMSIIPELGICPTCKSKFDEHLLTKLRRGPVVARKKRKLEDAESKGEEEAGNGAEKEMGTGAENRLDEAKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBQ6</accession>
    <name>A0A1A0HBQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_78493</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712079.1">
        <property type="nucleotide sequence ID" value="XM_018858888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBQ6"/>
    <dbReference type="GeneID" id="30031864"/>
    <dbReference type="OrthoDB" id="4017072at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="665" mass="76279" checksum="6FCC53D38D727182" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBW8</accession>
    <name>A0A1A0HBW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41596</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712016.1">
        <property type="nucleotide sequence ID" value="XM_018857432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBW8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBW8"/>
    <dbReference type="GeneID" id="30030408"/>
    <dbReference type="OrthoDB" id="447842at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18886">
        <property type="entry name" value="NUDIX_MutT_Nudt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43758">
        <property type="entry name" value="7,8-DIHYDRO-8-OXOGUANINE TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43758:SF2">
        <property type="entry name" value="OXIDIZED PURINE NUCLEOSIDE TRIPHOSPHATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="6">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="171" mass="19553" checksum="50DBB29C951813A9" modified="2016-10-05" version="1">MSGAQRQKYTLGFIRSDTNHVLLLNRQKAPWMGRWNGVGGKLDAGESPYECIVRETMEETGLMLPQYEDRGVMRWVRDGKECGGVHVFTAEVLEEEMQQYPTPRRHCHEGILDWKAMEWVLHEDNSGVVDNVQRMLVDLFGAGPHARWVSRYEGLQLVSCTYNGAGRRGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBX2</accession>
    <name>A0A1A0HBX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711898.1">
        <property type="nucleotide sequence ID" value="XM_018855946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBX2"/>
    <dbReference type="GeneID" id="30028922"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="100" mass="11207" checksum="05B5E98234365E40" modified="2016-10-05" version="1">MLNNPAEGKSPHLKASTPSLIPDLMSSNPYGAMHPIEETAAWTLYHDQHTKPPPYRKYDFGVAYRKIGRVYLVLGCLIVFGICAWKLVYFLARQISGGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC07</accession>
    <name>A0A1A0HC07_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Ubiquitin-like modifier-activating enzyme ATG7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_42304</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Autophagy is essential for maintenance of amino acid levels and protein synthesis under nitrogen starvation. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Preautophagosomal structure</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the ATG7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712025.1">
        <property type="nucleotide sequence ID" value="XM_018857527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC07"/>
    <dbReference type="STRING" id="869754.A0A1A0HC07"/>
    <dbReference type="GeneID" id="30030503"/>
    <dbReference type="OrthoDB" id="338614at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097632">
        <property type="term" value="C:extrinsic component of phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019778">
        <property type="term" value="F:Atg12 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019779">
        <property type="term" value="F:Atg8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032446">
        <property type="term" value="P:protein modification by small protein conjugation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000243">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.100">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.70">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme ATG7 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006285">
        <property type="entry name" value="Atg7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032197">
        <property type="entry name" value="Atg7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042522">
        <property type="entry name" value="Atg7_N_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042523">
        <property type="entry name" value="Atg7_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01381">
        <property type="entry name" value="E1_like_apg7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF3">
        <property type="entry name" value="UBIQUITIN-LIKE MODIFIER-ACTIVATING ENZYME ATG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16420">
        <property type="entry name" value="ATG7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like modifier-activating enzyme Atg7 N-terminal" evidence="11">
        <location>
            <begin position="6"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="10">
        <location>
            <begin position="296"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606285-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="629" mass="69993" checksum="800C60F93E05B88C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC18</accession>
    <name>A0A1A0HC18_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_42762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712066.1">
        <property type="nucleotide sequence ID" value="XM_018857583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC18"/>
    <dbReference type="GeneID" id="30030559"/>
    <dbReference type="OrthoDB" id="4010849at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="83" mass="9360" checksum="3D863DAF9DD888F1" modified="2016-10-05" version="1">MAPKLTKQQKADLVLRLCALKEAQARHLRTAVHKAAVGAERKVHRRVKKVSMTVRSVPILEVLNYERRASPTIAEIGKLARRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC66</accession>
    <name>A0A1A0HC66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoglycerate mutase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_41810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711977.1">
        <property type="nucleotide sequence ID" value="XM_018857454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC66"/>
    <dbReference type="STRING" id="869754.A0A1A0HC66"/>
    <dbReference type="GeneID" id="30030430"/>
    <dbReference type="OrthoDB" id="4818801at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050278">
        <property type="term" value="F:sedoheptulose-bisphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046390">
        <property type="term" value="P:ribose phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF15">
        <property type="entry name" value="SEDOHEPTULOSE 1,7-BISPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000709">
        <property type="entry name" value="6PFK_2-Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="238" mass="26812" checksum="AB6306BD34CD0CF0" modified="2016-10-05" version="1">MPTPRLIFVRHGQTEWSKSGQYTSVTDLELTKFGVSQMRNTGKHLIGNTPFQMIKPADLKLIITSPRKRAKQTADLLLESLDDEAKANISISEDNDLREWEYGDYEGLTTPQILDLRKQRGLDQDGVRWTIFQKGCENGEDHLQVTERIDRLIARIRTVHAKAIEDNAACDVLIVAHGHILRCFAARWVGRPLNVNPDMVLDAGGVGVLSYQHCNINEPAFYLSGAFTVPVDQEESEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCC0</accession>
    <name>A0A1A0HCC0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_22107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712150.1">
        <property type="nucleotide sequence ID" value="XM_018855139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCC0"/>
    <dbReference type="GeneID" id="30028115"/>
    <dbReference type="OrthoDB" id="4088185at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="94" mass="10877" checksum="6A51AA39CE15BE3D" modified="2016-10-05" version="1" fragment="single">TATAAEPVQPNMETVAILKRSMASSSRLIGTFTALKTTYIKLCKEFNFLLGKFNENERIKIELIHENNELRKLLYETIKEKELDRKQYKAELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCH4</accession>
    <name>A0A1A0HCH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Coatomer subunit zeta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_38676</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Golgi apparatus membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasmic vesicle</location>
            <location evidence="12">COPI-coated vesicle membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712310.1">
        <property type="nucleotide sequence ID" value="XM_018857028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCH4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCH4"/>
    <dbReference type="GeneID" id="30030004"/>
    <dbReference type="OrthoDB" id="10249988at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000013">
        <property type="entry name" value="Coatomer subunit zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039652">
        <property type="entry name" value="Coatomer_zeta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043:SF0">
        <property type="entry name" value="COATOMER SUBUNIT ZETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11043">
        <property type="entry name" value="ZETA-COAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="13">
        <location>
            <begin position="10"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="191" mass="21129" checksum="0F16DD01606FAE2B" modified="2016-10-05" version="1">MSLNISLYTVSAALILGPEGQRLYAKYYQISDAAQAYKTPQQQATFEKAIYSKINKQHQDLILYDNHLVTYKQTNDVILVIVSALNENESMLYSLTNNINEALTILLGNSLDKSVVLLKYDMVCLCMDETIEDGIIIEIDPAIIVSRVTNPPSGSAVASDMNMNIDLSEKGLFGALSFASKKISERLQQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCQ3</accession>
    <name>A0A1A0HCQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Respiratory supercomplex factor 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32150</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712182.1">
        <property type="nucleotide sequence ID" value="XM_018855991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCQ3"/>
    <dbReference type="GeneID" id="30028967"/>
    <dbReference type="OrthoDB" id="6604018at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098803">
        <property type="term" value="C:respiratory chain complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010155">
        <property type="term" value="P:regulation of proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007667">
        <property type="entry name" value="Hypoxia_induced_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050355">
        <property type="entry name" value="RCF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297:SF3">
        <property type="entry name" value="HIG1 DOMAIN FAMILY MEMBER 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12297">
        <property type="entry name" value="HYPOXIA-INDUCBILE GENE 1 HIG1 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04588">
        <property type="entry name" value="HIG_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51503">
        <property type="entry name" value="HIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="HIG1" evidence="10">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="16493" checksum="A86EE83DDF1AD44A" modified="2016-10-05" version="1">MDTIQKLVSKSKQQPLVPIGTLLTTAAVILAAKALKQGRKLDTQKYFRYRVGFQAFTLVALVVGGYYYQKETAEQKKSREDLAREKAKARELLWIEELERRDALIQSRKQRLEESKKELLKVALEGFEQERSQAESEDKSEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCT0</accession>
    <name>A0A1A0HCT0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar ATP synthase subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_12203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712238.1">
        <property type="nucleotide sequence ID" value="XM_018854191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCT0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCT0"/>
    <dbReference type="GeneID" id="30027167"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="226" mass="25165" checksum="199E1DC09E6D1ACB" modified="2016-10-05" version="1">MSLTDDQVNAELRKMKAFIEKEAQEKAKEIRLKADEEYEIEKASIVRSETAAIDSAYEQKLKKASLAQQITKSTIGNKTRLKVLAEKERILEDIFEEAGKSLVKLTSNKEEYKPILEGLIEEGLYAILEDTVTLIVREADLDLAKEAAETAAKTFAEKAKFEVTVKIDDKNFLDSALAGGVVVVNKSGKIEVDNTLDERLKLLSEEALPGVRLELLGPSASRKFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCV9</accession>
    <name>A0A1A0HCV9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Initiator tRNA phosphoribosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10771</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712342.1">
        <property type="nucleotide sequence ID" value="XM_018853850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCV9"/>
    <dbReference type="GeneID" id="30026826"/>
    <dbReference type="OrthoDB" id="45256at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043399">
        <property type="term" value="F:tRNA adenosine(64)-2'-O-ribosylphosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019988">
        <property type="term" value="P:charged-tRNA amino acid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007306">
        <property type="entry name" value="Rit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033421">
        <property type="entry name" value="Rit1_DUSP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033449">
        <property type="entry name" value="Rit1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31811:SF0">
        <property type="entry name" value="TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04179">
        <property type="entry name" value="Init_tRNA_PT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17184">
        <property type="entry name" value="Rit1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007747">
        <property type="entry name" value="Ribosyl_Ptfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rit1 N-terminal" evidence="2">
        <location>
            <begin position="10"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Rit1 DUSP-like" evidence="1">
        <location>
            <begin position="367"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="469" mass="53100" checksum="D843FD2B5697FFC5" modified="2016-10-05" version="1">MEYSQILKDLRRESATVRNRIQSIIFDAKYVAQFSEFPIVANERCGLWYVEPKNTAASVYFKSTDGHTGQWKFSLRRLNFHMLDLFQLSQTIVLVDSTRKGKIMPDALLKTVPMWCAVLNYIMFEKEDVDQWDELAKDNWLCTPPEMVSASEHSAMVKLIPEHAREVQKLGLVVKQELIQKLGAHKPLVPHWLYPGKKSRVSASDNQFSICCLTASRKTSSGTNVPGWHYTQPYVQGAADDHELWASPEICEGKLDHNLLWNEIYYEPADDLRVVDMATGDICLWLGEDEFLSRIEKIYTNSKSCKDTSAMDVSPLKNTGISIGAITQDLSFSEITKINDLISHIVVLSSEFAVTNVPKDSSVKIILHRVESSKKGSKQLREILPKIFETISTVSMQDQVIILCDSGKDLCVGLALAILAKYFDRDWNLVSSLPHVNKDVIKQHLCMVLSCRQVNPSRNTLQSVNTILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCW4</accession>
    <name>A0A1A0HCW4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_191368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712232.1">
        <property type="nucleotide sequence ID" value="XM_018854846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCW4"/>
    <dbReference type="GeneID" id="30027822"/>
    <dbReference type="OrthoDB" id="4087931at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="398"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="410" mass="45794" checksum="C6810974196EFBE9" modified="2016-10-05" version="1">MNRGTTSCHSLSSLSYAMPRGRSSPMNGPVFAPKPGFDTASTTLENSPFFRETGALYDPINNLHAELPVQKPMVANSNSTLVSEPSFNHPFESPVSPQNFPTLSLFHEPDIKDDIFQDQEQLWSPEVNFETRSSSTTASQLLPNEPIRPAFEHLDKANSIMPFGLKEKSVSCYTNSNEDGISSTIGAMSSEIPDIPVFHTFSTPHQYCNISGDENSQCDFEMFLGRNSFKLEGMKEIVPDCKNIDKALANNGENSKKNVKLGESSPTFRMQTFSAIDEHAHSRHHAFQRVGSYGSGYKASSKLSLDNCLHEFHMKEGKFMFADETAMILKLLKTPRKLVTSRMSPSKVSNTLQRSKNPRMISKPSPWAGLRRRREVSKDMKSRLGSFQVQLKNDHNLSSTSDESFSTESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD39</accession>
    <name>A0A1A0HD39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_39643</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DOP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712386.1">
        <property type="nucleotide sequence ID" value="XM_018857162.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HD39"/>
    <dbReference type="GeneID" id="30030138"/>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056458">
        <property type="entry name" value="TPR_DOP1_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24597">
        <property type="entry name" value="TPR_DOP1_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="7">
        <location>
            <begin position="28"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like middle TPR" evidence="8">
        <location>
            <begin position="353"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="9">
        <location>
            <begin position="1226"/>
            <end position="1664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1686" mass="190251" checksum="AE1599563B4DD3D4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDA2</accession>
    <name>A0A1A0HDA2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_77563</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712555.1">
        <property type="nucleotide sequence ID" value="XM_018858723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDA2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDA2"/>
    <dbReference type="GeneID" id="30031699"/>
    <dbReference type="OrthoDB" id="5368863at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21077">
        <property type="entry name" value="DBD_Rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022652">
        <property type="entry name" value="Znf_XPA_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00598">
        <property type="entry name" value="rad14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01286">
        <property type="entry name" value="XPA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="11">
        <location>
            <begin position="261"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="186"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="405" mass="46215" checksum="E1A78188E2AE0D30" modified="2016-10-05" version="1">MAGLTDEQRKRIDENRRKALERLQQRKAGTLGARGSPAPSAKTSSMSNSAQHNSSSGAQDAMSRSGQDAIVGSGQQAIARGAHDAMQAATGPGQQHVSIPELPRVELTREQKARIEQNRERALQIQKEQLRTLRDASGPLGPVAVVTQQDRKRLKPEFNPPAIQKKDYIEYDFATMKDSRGGFLDESQKHGLKGPDEEPQSLKDWKEKQKQDLAVKDLAPPLDLSSAPRCVECESLEIDANLKTNFNVRVCRRCMREKPDKYALLTKTECREDYLLTEPELQDTGLLRRIEKPNPHGFLRMQLFLRLQVEEYAWKKWGSAEGLDAEWERREKARLDRREKRYQAKMREMRKKTRAEEYTRKLRNGQGLGDRHTHDWSAPLAVGGNDRLVRRRCVDCGIETEEIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDE7</accession>
    <name>A0A1A0HDE7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_28259</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712449.1">
        <property type="nucleotide sequence ID" value="XM_018855351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDE7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDE7"/>
    <dbReference type="GeneID" id="30028327"/>
    <dbReference type="OrthoDB" id="3366990at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043035">
        <property type="term" value="F:chromatin insulator sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032211">
        <property type="term" value="P:negative regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001015">
        <property type="term" value="P:snoRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010833">
        <property type="term" value="P:telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000137">
        <property type="entry name" value="MYB DNA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013867">
        <property type="entry name" value="Telomere_rpt-bd_fac_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052833">
        <property type="entry name" value="Telomeric_DNA-bd_trans-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47807:SF1">
        <property type="entry name" value="PROTEIN TBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08558">
        <property type="entry name" value="TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="408"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="412"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="325"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="500"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="332"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="520" mass="57537" checksum="053C5F4D9ADD8544" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE04</accession>
    <name>A0A1A0HE04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712825.1">
        <property type="nucleotide sequence ID" value="XM_018857037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE04"/>
    <dbReference type="STRING" id="869754.A0A1A0HE04"/>
    <dbReference type="GeneID" id="30030013"/>
    <dbReference type="OrthoDB" id="4018688at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291760" description="Survival protein SurE-like phosphatase/nucleotidase domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="447" mass="49129" checksum="4DECFDC365A3CAD3" modified="2016-10-05" version="1">MRLSIISSFCTLLGALPLAASLNILISSTDSWVEKNARYLHPALVEAGHTVVFVGPLHPNLVNSAGGLFQAQKPGQRRKASELPDYEDAYSAGDFGHLKEAHQKYYTYTRKLNALLRGAKGVIQKKESVEFDAEFVKETSELVRNPTYGQDPLNPDFWYVNGSPLEALLVAFDVILPTHVSGFSPDLVLLGPNEGLHLTCSDSPDVSGIPITDLLALKDELEAMRLLAQVKNHRVISVSTEDSHHIYYEDEGFFNVEETSYDKLFKANFVTQNIQFVNLRLISLVDKVVLHMETGQALNVNFPSMNKRLSTCSTSRGVGPQFSQVASSTGKRNMMGKIFAIPQLSLGDDCVEMAQKNLFKVSEALDGAAELSDLEFTRLQHLLAPSDGITVGPDARQSVSGSDETQMRACNADEYKALELCQIAVSVNDLARGSSLSEEILDVVRYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEK2</accession>
    <name>A0A1A0HEK2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712829.1">
        <property type="nucleotide sequence ID" value="XM_018855820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEK2"/>
    <dbReference type="GeneID" id="30028796"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="14109" checksum="1EA90BA78A708170" modified="2016-10-05" version="1" fragment="single">FCESTLGTLSIHTLELISFHGLRSIFDAFFQAPHMKDNTPKKILNQLHIFLLSGKLAMKDFHSVYPPFIAAPQFRIWSPTTLLSVALFSLTNVDRTGNSAFCMVWPQPKDYYETEFSCLLNYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEL2</accession>
    <name>A0A1A0HEL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_11379</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713055.1">
        <property type="nucleotide sequence ID" value="XM_018853970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEL2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEL2"/>
    <dbReference type="GeneID" id="30026946"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000919">
        <property type="entry name" value="NPR1p Protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF113">
        <property type="entry name" value="NITROGEN PERMEASE REACTIVATOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="428"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="369"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="369"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="787" mass="83889" checksum="8B1F644B508C8F0A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HER2</accession>
    <name>A0A1A0HER2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">FAD/NAD(P)-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_77793</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the paxM FAD-dependent monooxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712873.1">
        <property type="nucleotide sequence ID" value="XM_018858767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HER2"/>
    <dbReference type="STRING" id="869754.A0A1A0HER2"/>
    <dbReference type="GeneID" id="30031743"/>
    <dbReference type="OrthoDB" id="9993796at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050493">
        <property type="entry name" value="FAD-dep_Monooxygenase_BioMet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789:SF172">
        <property type="entry name" value="HYDROXYLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13789">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding" evidence="6">
        <location>
            <begin position="10"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="417" mass="46242" checksum="BEA3A35B0BA89A93" modified="2016-10-05" version="1">MTSDLWKPLTVAVLGGGLGGLSAAIAMRRAGHNVSIYERNDYAGEVGASLSCASNGGRWLHDWGVDPYEANPVILKNLIRHDWKSGKITGKYSLGDYQAKFGFPYYNFHRIDIHTVLHKYAVSEDGVGTPCELHVNHKAIKVDYEAGHVEFENGSQCTADLIIAADGIRSLTKPQLGISPKFRHQDSSCIRVLFDTKKVQELGLADFLENEAIEFWGGDDKNKIVLSPCADKRVVSCYCFYAASPEDVKDGWHNEILVQQLLDTVPHLDPRLVELFTTCGYDIKQWRLYVHEPLPFFYKSSANGSRGVALLGDAAHAMMPDQSQGACAAFEDAGALGYIFSEKFHLSVAEGLRVYEQERKPRVTRIQKASLRARENLNERIGWSSGASDIETAQKLTIEEVCGYDMKGHIDDLVAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEY2</accession>
    <name>A0A1A0HEY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31361</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712964.1">
        <property type="nucleotide sequence ID" value="XM_018855835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEY2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEY2"/>
    <dbReference type="GeneID" id="30028811"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="13"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="345" mass="37268" checksum="A49148D776F4CBD8" modified="2016-10-05" version="1">MTLGIRAAAISYLLSLVFVMIIRNDTVEIDPGAKSLEGLLICTRVSYSIINSPSLTLQSNVYNGGRFSVSSTDELGASVLMTGRRFDNYGLIVFNSLCVQADCLYDIDAKEAFLNNGTMFFGFFGASTKSSNMYVTSVVSWENTGTMIFMQSPGLSTSLLIGDFQRLTRDFSITNKGVICFRNLVWLVTTPIGGDGCINVGSGSILEMHFPDFSPKPNQIINLDSSDSVLRALGLEELLDVIPVIKITGLGGGNSIEIDLPYEESISTGYCTTTGRLSLGIAHKNRVFFEVGPGYNVSDFKTQKTLVGNKITVDDPSPHEPPLACKCDTDFYSAPAANSSQVPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF47</accession>
    <name>A0A1A0HF47_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_11420</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713101.1">
        <property type="nucleotide sequence ID" value="XM_018853980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF47"/>
    <dbReference type="STRING" id="869754.A0A1A0HF47"/>
    <dbReference type="GeneID" id="30026956"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="292" mass="32764" checksum="0331E007FD24503E" modified="2016-10-05" version="1">MPGLQDNTKTYTLNDGHEIPAVGLGTWRATSEDNIADVVESALRNGYRHIDTAAIYGNEEDVGKGIKASGVPREEIFVTTKLWNSDHKNVRKAFEESLRKLQLDYVDLYLIHWPLDGDFDDESANISDCFSTYIELQKLKQEGKAKSIGVSNFSKGKIESLLAHPEVTIKPAVNQIEAHPLLPQYELYDYLKKNDIYIEAYSPLGSADSPLFKEKNILRIAQKNDVEPAQVLISWAIQRDTVVLPKSVKASRLVSNIKTFELSDEDFAVLNELSEVEGVVRTNNPLPALFKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF73</accession>
    <name>A0A1A0HF73_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">LMWPc-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_31430</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713038.1">
        <property type="nucleotide sequence ID" value="XM_018855849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF73"/>
    <dbReference type="STRING" id="869754.A0A1A0HF73"/>
    <dbReference type="GeneID" id="30028825"/>
    <dbReference type="OrthoDB" id="3388at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16343">
        <property type="entry name" value="LMWPTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000105">
        <property type="entry name" value="Low molecular weight phosphotyrosine protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF7">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="9">
        <location>
            <begin position="7"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="162" mass="18331" checksum="C14ECA05D2ACED99" modified="2016-10-05" version="1">MAQDKKISVAFVCLGNICRSPMAEAVFTHKVHELGYTNQVELIDSFGTSGWHIGESPDSRSASTCRKHGVAVKHAAQQITPKDFERFDYVIGMDEQNKSDLLHMRPRDSKAKVAIFGKWQTSPEFAEIVLDPYYGGRDGFETNFKQISHFSEEFLKQELGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFF6</accession>
    <name>A0A1A0HFF6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_39895</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713094.1">
        <property type="nucleotide sequence ID" value="XM_018857196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFF6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFF6"/>
    <dbReference type="GeneID" id="30030172"/>
    <dbReference type="OrthoDB" id="411251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF1">
        <property type="entry name" value="PROTEIN YIPF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="8">
        <location>
            <begin position="150"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="30368" checksum="10790440B06EC8F8" modified="2016-10-05" version="1">MSWLDPENNDYIIPDEPAPEPAAPAGARGRGAHNHSPGTETAAPGFPWTPAVDLLAAFTPFFAPKTGPAAPRVSERRFAGGDTLDEPILETLKRDMLQIGRRVAAVVWPAQLQTRLAAVAAAGGPVAAAAAAEAPGHVTHEAPGGLLPLDWDLWGPLVFLLAYAVTMGAALPAAQTNMVFSGTFLLMWMFYLVVGFNVQLLGGTVSFLAAISAAGYLMFPLVAGALVSTAAVPWRMARLVVMAFCAVWSIYAASVCLRCLGVLPGRLFLAIYPVTLMYAVLAWLVVIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFI6</accession>
    <name>A0A1A0HFI6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_38153</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
            <location evidence="1 9">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713143.1">
        <property type="nucleotide sequence ID" value="XM_018856962.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HFI6"/>
    <dbReference type="GeneID" id="30029938"/>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033553">
        <property type="term" value="C:rDNA heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 9" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BP28 C-terminal" evidence="10">
        <location>
            <begin position="1583"/>
            <end position="1733"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="1826"/>
            <end position="1864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1866" mass="207915" checksum="295F33735A9433F4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFP5</accession>
    <name>A0A1A0HFP5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_35846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713159.1">
        <property type="nucleotide sequence ID" value="XM_018856660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFP5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFP5"/>
    <dbReference type="GeneID" id="30029636"/>
    <dbReference type="OrthoDB" id="4092564at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="76" mass="8330" checksum="395D4FAEFAD7897C" modified="2016-10-05" version="1">MDFGNLINKGKEALAGEDGKIDYSGMQKDAQEAYKTFSTTEGSYTDKASAAYSEYQKDHASQKSSDETKSDSTEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFT3</accession>
    <name>A0A1A0HFT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37337</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713342.1">
        <property type="nucleotide sequence ID" value="XM_018856859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFT3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFT3"/>
    <dbReference type="GeneID" id="30029835"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.70">
        <property type="entry name" value="Transcription termination and cleavage factor, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026896">
        <property type="entry name" value="CSTF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038192">
        <property type="entry name" value="CSTF_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14304">
        <property type="entry name" value="CSTF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="12"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="278"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="366" mass="39622" checksum="F3AE0D2247638153" modified="2016-10-05" version="1">MRRNESQQPNLSTIYVGLIPFDWSEDNFKAVVCGLGNVYDVRLGFDHIGKNKGFAFVEFETPQQAVNAAQLLTKVQIVMPSLGQVRRLRTEVKETFRSNDQRAIIPLKPEHLPAGVQFPPEVVVKKPQLLWASQAKYASFSGPDIPSNTPNSRGASHTPPLPGNMGNMGSIAPPNSSNTLPGELLKATHYLALPKKLPFSTPDKINETLGQISPPQLIELIANLKNIMNSSDSARAAEVFRLSPNLAASATQALLLMGFIDEEVIQEAMKAEIGNATPAAPITPQQQNIHPGPSHQNLPLLPPAPTGPSTPSRWPNLSLLAQMKLSALPSDQAELIAQVLALSPEQISALPSDRQSMVAGIRQQYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFT9</accession>
    <name>A0A1A0HFT9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Multiple RNA-binding domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_9348</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM MRD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713505.1">
        <property type="nucleotide sequence ID" value="XM_018859363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFT9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFT9"/>
    <dbReference type="GeneID" id="30032338"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034462">
        <property type="term" value="P:small-subunit processome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12565">
        <property type="entry name" value="RRM1_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000247">
        <property type="entry name" value="Multiple RNA-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000459">
        <property type="entry name" value="Multiple RNA-binding domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="2"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="322"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="505"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="627"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="728"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="197"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="853" mass="95242" checksum="DFB1B103F760443F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFU1</accession>
    <name>A0A1A0HFU1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Chitin synthase</fullName>
            <ecNumber evidence="2 10">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_34831</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the chitin synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713204.1">
        <property type="nucleotide sequence ID" value="XM_018856529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFU1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFU1"/>
    <dbReference type="GeneID" id="30029505"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="551"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="578"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="630"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="751"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="797"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="11">
        <location>
            <begin position="69"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="836" mass="94757" checksum="1F848BE5763293A5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFZ5</accession>
    <name>A0A1A0HFZ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallo-hydrolase/oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713386.1">
        <property type="nucleotide sequence ID" value="XM_018856526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFZ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFZ5"/>
    <dbReference type="GeneID" id="30029502"/>
    <dbReference type="OrthoDB" id="332863at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070290">
        <property type="term" value="F:N-acylphosphatidylethanolamine-specific phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070291">
        <property type="term" value="P:N-acylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070292">
        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024884">
        <property type="entry name" value="NAPE-PLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15032:SF4">
        <property type="entry name" value="N-ACYL-PHOSPHATIDYLETHANOLAMINE-HYDROLYZING PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038896">
        <property type="entry name" value="NAPE-PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="179"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="448" mass="50972" checksum="719203E6261DECA1" modified="2016-10-05" version="1">MTSFFRFTRGTRIGIGALVSYTSFESYMYIKTAHLIQERKDAIDSSKSDENDGRLRFQSATINGIYVNPFEEYRPQTAFEFVYVRILEVLESIYGNKVEMHDPHVTSTGEIAEVEQFLQTFKPNYELLRYNSKILENCLHSGSYEALLNTESKSVPINQQLLFTWLGQSCSLLQISGINILTDPIFSDYLFANNVGPKRLVNSPMSLEDVNYATNDNLNLVLVSHNHPDHLEMSSAQKIGNSALWVVPLGLKLVLQRKGIDNVIEMDWWDVLPLNDHLFGERGSDFPDNYELVFLPAMHWSGRYVVDANTSLWGSFMIRKNGTSLVYHAGDTGFSKELFDKIGQNYGPVTLSLLPIGQYCPSWHQKPRHVSPEECFKIASQIKTSNVMGVHFGTFKLSSEPILEPKELFMKLAELRNKTPSYSVPEFGFTYEYDINRSGQVAENTSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG88</accession>
    <name>A0A1A0HG88_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_96313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713486.1">
        <property type="nucleotide sequence ID" value="XM_018859428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG88"/>
    <dbReference type="GeneID" id="30032403"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="193" mass="21694" checksum="8944C76110BF48DA" modified="2016-10-05" version="1">MEAVLISMSRARHRSRKSRNPLSSICLRFIKDLALSSPCCTCLDFHSAALVTVAATIRFFFKLRPTNPSTASAVSPMTFARGLGLSNCAIFAVVPFIPLRKVSTERWLGIWTTALESWPSLPRARANKFLISRIVLIAFQSPKRNSEAKYGEEEPSERIFVAQKWMRKSAPDTAWPDPVRPIGCFTVMDYLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGF0</accession>
    <name>A0A1A0HGF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713438.1">
        <property type="nucleotide sequence ID" value="XM_018855423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGF0"/>
    <dbReference type="GeneID" id="30028399"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="472" mass="53560" checksum="E68B8D7A89A968F4" modified="2016-10-05" version="1">MPLTINSISTDDIKVTAFPLVRKYRPGRTSHGLFNLTRKRIFPFLESPVLPSAGSSLLMGAHRGGQWSDGNRYSLSSQNDIASEEKVNIAVTTALKYNLEKLISKTNKKIEKFSHTHSNAFKNKESHTKVWKKCRKLIDRMINGRKELILTYSVITPGSDTTETRIWDGEWVDFTGERTTVSSHASVKFGTLSLKGFFVNFVETIRNLIEKRAYFGKIFGEDSVFRLNLLNIRIPPLLELIKRFAKWWTMDEGVKTYPTYHCDNSIKRNTRVDNLTLLLEALDRMDAEEAALAAANGTSSQTGPILESEDIPSLYLPLPDIPSALSVLDFEQPFEEHFQDSFENMGATLGYDVEDYQSLYTHATPEYICSGNVQETNECFEDDLLANFKAHDLYESSYSPISVSDIPLSASSNLEWRGRQANNPIDQLFSPMYHVETDSLNLKEENSGGFLKKLFRAMLLAERQPKSTLSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGN1</accession>
    <name>A0A1A0HGN1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Exoribonuclease phosphorolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_37274</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713518.1">
        <property type="nucleotide sequence ID" value="XM_018856849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGN1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGN1"/>
    <dbReference type="GeneID" id="30029825"/>
    <dbReference type="OrthoDB" id="2504340at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF2">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT MTR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="37"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="272" mass="29433" checksum="A1C65D7EA3820D0C" modified="2016-10-05" version="1">MSTDRRRILGPADAVRPVAAPPVSAENTARHHPDEPVRKFFIKTGLSKNANGLAYLEIDDTIIEVGIFGPRPIKGLFVNRASFTVETKFLPHVTQPSEVLFNGSQAAANGRLLLTNIEQKLSTFVETAFLPAVLLEKYPKSTIDVFVNVLAFNANTSSMLNLAAWIINCTSLAMVDSGIEVRDMVTCGHVKTCSGLAEMDALIATDSDAEKGTECVASFMAMHNNDMVAVWVQGSDGDGVSESVMLQLLEGCQEMSTIVRKNLNGFLLDLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGQ4</accession>
    <name>A0A1A0HGQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29698</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713651.1">
        <property type="nucleotide sequence ID" value="XM_018855461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGQ4"/>
    <dbReference type="GeneID" id="30028437"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="493"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="428"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="434"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="877" mass="98182" checksum="9C9970D77CD379AE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGT2</accession>
    <name>A0A1A0HGT2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_29868</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713865.1">
        <property type="nucleotide sequence ID" value="XM_018855502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGT2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGT2"/>
    <dbReference type="GeneID" id="30028478"/>
    <dbReference type="OrthoDB" id="265044at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="344"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="344"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="45651" checksum="DD49AF51AABAA9E7" modified="2016-10-05" version="1">MLTIILQSQEFATSMRHTLRLTFDSSIPTYRLLFVACSDQRCALKVLSRWLCSIVFTPLNNLLSAINFIFSPFKPSVYSKSQQFTDTKRPQNASKLFIPTYVMYTIVDGITTMNSPFDILVIGQRGVGKTTLLRKYVMGARDVPEFELSECLVYKSVERPCHCLNSSSVTSETTINKHISILNSSAGVDTLLTQNSYQVNNAQTMVFAYAAASSESFEALEYTINCIESVRDKLPPCVIVSLKPDLNQMDQVLASQGEALAKSCGALLFVEADLYDDRPVDNVFAPLLEVLFLKDEHQNCLPTNLGNTEALSNDLKDFLVDEVDNLKEDSFVLKRMAEITLKRTSPTSSRSSAAKSENPSEVSVHQQPTEKLNEFRTASLKSLESSFRTSRLSQAPGSKVDKATSSCCNIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGV6</accession>
    <name>A0A1A0HGV6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Golgi vesicle protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_76434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713890.1">
        <property type="nucleotide sequence ID" value="XM_018858528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGV6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGV6"/>
    <dbReference type="GeneID" id="30031504"/>
    <dbReference type="OrthoDB" id="5549748at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07600">
        <property type="entry name" value="BAR_Gvp36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="36033" checksum="5FF3C7D5C0A1665A" modified="2016-10-05" version="1">MDFNFSAFKNNLKSFGDKVSIEFNNEVLPFAQRTTRLVQERMGNVNVDEISQLPSEYVELANRCNNVEKLYKNILKVTQNYENESYDYPINLQESFTEFGKNISDRFSNLSKATTPAEAQAALVTPNGEFKPPKTMYHALARATDGSILTASADANQDPLIKALDLYSSNLSKIANARLGQDQLIKTKFNAPLSTTLRSLLGQSSSIQKRVEEKRINYDVSRQALASCSNPSKEPQLRVSMENAEDDFANTVEDALSVMQNVLEQAKPIEEILELVKAQLAYHKLASELLGGMIGDIEGLADENSKLQGSQLSQRNSESGDFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH87</accession>
    <name>A0A1A0HH87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UNC-45/Cro1/She4 central domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_15475</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713723.1">
        <property type="nucleotide sequence ID" value="XM_018854600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH87"/>
    <dbReference type="STRING" id="869754.A0A1A0HH87"/>
    <dbReference type="GeneID" id="30027576"/>
    <dbReference type="OrthoDB" id="5574718at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF1">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UNC-45/Cro1/She4 central" evidence="3">
        <location>
            <begin position="3"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="629" mass="69331" checksum="4A9D1DB046D42C7E" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHA5</accession>
    <name>A0A1A0HHA5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyoverdine/dityrosine biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713862.1">
        <property type="nucleotide sequence ID" value="XM_018856719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHA5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHA5"/>
    <dbReference type="GeneID" id="30029695"/>
    <dbReference type="OrthoDB" id="429813at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007817">
        <property type="entry name" value="Isocyanide_synthase_DIT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37285">
        <property type="entry name" value="SPORE WALL MATURATION PROTEIN DIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37285:SF5">
        <property type="entry name" value="SPORE WALL MATURATION PROTEIN DIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05141">
        <property type="entry name" value="DIT1_PvcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="54009" checksum="DFC1DD1B5517D37F" modified="2016-10-05" version="1">MTVQDKTVYERVHYVYCRNEDSFLNCRIQKEATKSEAEAWSCLQKAEALDPKISSELLDMTIKGCSELMFFEKRAGNIVVGVATEVFEPTVFDAWFLQTILENGDFSFNRKVSGKFKELAVEFTEIFENSIKNAARVDRWEEGRESFIENVRFFTSRQIPIEAVLPAFPCKTQNRDKAQEGKPDMGEELALRRIIDFVQAVNKVYAPGMKFYIVSDGHVFSDCIGVDDNVVDTYTAQLIEMYERIKPEDFNGIFFKGLNDCFKSSSKNVIATHLEDIKIDHHLDTMVDEETDVNRKILMFGCDDNDNLLREQIKTPGHARLFLYRGFNKFMSEDLGRTTAAQKMSGKKFKKMVSLVSYEMIRRNDAYSNLVELVFPFHLRLSIHAHPNCGPKFGIRLLNPEICSTGCHNQDEEDRLLHIPTPWHNAIFKVSDAPKMIISPAKLAREYEENEGYTGGWDEAQRCFVFTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHC2</accession>
    <name>A0A1A0HHC2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calcofluor white hypersensitive protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_30016</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714060.1">
        <property type="nucleotide sequence ID" value="XM_018855533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHC2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHC2"/>
    <dbReference type="GeneID" id="30028509"/>
    <dbReference type="OrthoDB" id="68581at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000031">
        <property type="entry name" value="Calcofluor white hypersensitive protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057315">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051916">
        <property type="entry name" value="GPI-anchor_lipid_remodeler"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053912">
        <property type="entry name" value="PGAP2IP_TM_1nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053911">
        <property type="entry name" value="PGAP2IP_TM_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859">
        <property type="entry name" value="CALCOFLUOR WHITE HYPERSENSITIVE PROTEIN PRECURSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14859:SF1">
        <property type="entry name" value="PGAP2-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23022">
        <property type="entry name" value="6TM_1st_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23021">
        <property type="entry name" value="6TM_2nd_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23226">
        <property type="entry name" value="Exo_endo_phos_PGAP2IP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="416"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="564"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="600"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="639"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP first transmembrane" evidence="4">
        <location>
            <begin position="270"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP second transmembrane" evidence="3">
        <location>
            <begin position="442"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="PGAP2IP C-terminal nuclease-like" evidence="5">
        <location>
            <begin position="671"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="951" mass="106525" checksum="44EEB5A48D3217F6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI67</accession>
    <name>A0A1A0HI67_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pro-apoptotic serine protease NMA111</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Pro-apoptotic serine protease nma111</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_38135</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714058.1">
        <property type="nucleotide sequence ID" value="XM_018856959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI67"/>
    <dbReference type="STRING" id="869754.A0A1A0HI67"/>
    <dbReference type="GeneID" id="30029935"/>
    <dbReference type="OrthoDB" id="4217619at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120174">
        <property type="term" value="P:stress-induced homeostatically regulated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06786">
        <property type="entry name" value="cpPDZ1_ScNma111-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06719">
        <property type="entry name" value="PDZ2-4_Nma111p-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.120">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025926">
        <property type="entry name" value="PDZ-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366">
        <property type="entry name" value="PRO-APOPTOTIC SERINE PROTEASE NMA111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46366:SF8">
        <property type="entry name" value="PRO-APOPTOTIC SERINE PROTEASE NMA111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12812">
        <property type="entry name" value="PDZ_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13365">
        <property type="entry name" value="Trypsin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="278"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ-like" evidence="9">
        <location>
            <begin position="372"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ-like" evidence="9">
        <location>
            <begin position="851"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="975" mass="109158" checksum="E13CAAC65690894B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI89</accession>
    <name>A0A1A0HI89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor IIIC 90kDa subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_114661</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714365.1">
        <property type="nucleotide sequence ID" value="XM_018853998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI89"/>
    <dbReference type="GeneID" id="30026974"/>
    <dbReference type="OrthoDB" id="6021743at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024761">
        <property type="entry name" value="TFIIIC_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024764">
        <property type="entry name" value="TFIIIC_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12657">
        <property type="entry name" value="TFIIIC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12660">
        <property type="entry name" value="zf-TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor IIIC 90kDa subunit N-terminal" evidence="1">
        <location>
            <begin position="21"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC putative zinc-finger" evidence="2">
        <location>
            <begin position="658"/>
            <end position="724"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="732" mass="82350" checksum="3D035970749EDE25" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HII6</accession>
    <name>A0A1A0HII6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)</fullName>
            <ecNumber evidence="3">4.3.3.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_76771</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by a SNO isoform. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714306.1">
        <property type="nucleotide sequence ID" value="XM_018858582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HII6"/>
    <dbReference type="STRING" id="869754.A0A1A0HII6"/>
    <dbReference type="GeneID" id="30031558"/>
    <dbReference type="OrthoDB" id="1660966at2759"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000001">
        <property type="entry name" value="Pyridoxine biosynthesis protein PDX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="9">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR029271-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="292" mass="31311" checksum="3C36CFAEC6820B1D" modified="2016-10-05" version="1">MSDFKIKAGLAQMLKGGVIMDVVNVEQAVIAERAGACAVMALERIPADMRASGQVCRMSDPHMIKAIMAAVSIPVMAKVRIGHFVEAQVLQELGVDYIDESEVLTPADWEHHIEKDTFKVPFVCGAKDLGEALRRVNEGAAMLRTKGEAGTGDISEAIKHVRKVKAQIAQLLAHKDNAAFIEDTAKTYRVPVSLLQDLIDNEGKLPVVNFAAGGVATPGDAAICMQLGCDGVFVGSGIFKSDNPEKLAKAVVDAVTHYDDPKKILEVSTDLGQLMFGTSIQSISEDQRLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIJ4</accession>
    <name>A0A1A0HIJ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">SUR7-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_35793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714144.1">
        <property type="nucleotide sequence ID" value="XM_018856653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIJ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIJ4"/>
    <dbReference type="GeneID" id="30029629"/>
    <dbReference type="OrthoDB" id="5419460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36414:SF1">
        <property type="entry name" value="PROTEIN SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="30465" checksum="E9B2D64B3E0695EC" modified="2016-10-05" version="1">MKLIGTLLNLVLLSGSTLLLTFLVLSGSVNHFPFDTFYWVRGDTSAITDAYNESAWTFWGVCDYNDFSTCQSGPAYPISPVDNFGTTSGVPSDFVDNRDTFFYLSRFGFAFFLLALAFSGFALLTCFLGFCFSFIDKVMVVLAMIALFFVCGAAAFQTAVVVLARNAFANDDLLSLLGVKLLAMTWAAVACLLLVFFITCSSNIANSYRKHIERINEQNVYPNDDYAPEARNSQSALGDDSSFTRANVPEKNDADSGGIRFFRIKRNQKPSDDESV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIR7</accession>
    <name>A0A1A0HIR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76827</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714386.1">
        <property type="nucleotide sequence ID" value="XM_018858593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIR7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIR7"/>
    <dbReference type="GeneID" id="30031569"/>
    <dbReference type="OrthoDB" id="1854502at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000742">
        <property type="term" value="P:karyogamy involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13289">
        <property type="entry name" value="PH_Osh3p_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000001">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.680">
        <property type="entry name" value="GOLD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036598">
        <property type="entry name" value="GOLD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041680">
        <property type="entry name" value="PH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF203">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15409">
        <property type="entry name" value="PH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101576">
        <property type="entry name" value="Supernatant protein factor (SPF), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="6">
        <location>
            <begin position="207"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="443"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="853" mass="95855" checksum="A99D29B6F192349D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIS5</accession>
    <name>A0A1A0HIS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_76943</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714540.1">
        <property type="nucleotide sequence ID" value="XM_018858611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIS5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIS5"/>
    <dbReference type="GeneID" id="30031587"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="200"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="237"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="356" mass="38679" checksum="F33E9556C12F703F" modified="2016-10-05" version="1">MLAGKSQKELLKLARLIVQPLQLNFHKGQAGKVGIFGGCEDYTGAPFFASHSAALVGADLSHVICEKLAAPVIKGYSPDLMVHPYLYELLNPEVARFAPAETWAALLKKTPTEALKDELLGKIVDTHILPKVMALVDRIDVFVVGPGFGRDPLMLKTLQRILQEIMVANKPVVLDADSLFLILLDPLLIRGYKKAVITPNVVEFGRICSALGIENTDSVETAQKVSELLGGVVVVKKGAQEVIVRGQSCLLNDMEGSVRRVGGQGDTLTGAMATMLVWASHYQEGAWETPRYEHIENDDLALVACYAACALVRAASNKAFGRYKRSMQTSNIHEFLGEAYGELFEASGEQTIGKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIT1</accession>
    <name>A0A1A0HIT1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRM5</name>
        <name evidence="12" type="ORF">METBIDRAFT_30278</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714392.1">
        <property type="nucleotide sequence ID" value="XM_018855597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIT1"/>
    <dbReference type="STRING" id="869754.A0A1A0HIT1"/>
    <dbReference type="GeneID" id="30028573"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="134"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="261"/>
            <end position="262"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="289"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="461" mass="53007" checksum="66E4F23BD7C2EDD7" modified="2016-10-05" version="1">MNVLRKFSPPVNRQMRVLDKSFFQKEVSLLVAAFPNPKFLGDFVKSCKGDFLLMSGVKHIVPINGTKGVLLREDIDSLESYRDLLSSTALAKIAEYDVEILPYTLKLDYSFWKADDILHAVLPEHLTNDIPTGFAQAGHIAHFNLRSEFKEYGPLIGQVILDKNSKIETVVDKVDSIDTKFRTFKMKVLAGKDDFVVEQNESGCKFRFDFSSVYWNLRLSTEHDRLISQFQPNEVVGDVFAGVGPFAVPAGRKNALVLANDLNPESYKYLKENIRLNNVEDFVRPSNLDGRDFIRNSPSMILNWAKSESVIEKRKTVKRRKTDSNTQLATTEKSVEITQIQIPQFISNYVMNLPDSALTFLDEFVGLYTHDEEVERIAKSDPNFRLPIINVHCFEKYSSDEPEPTMDDLHRRVHSRIIEYIQYSAPFEAFNFHLVRKVAPTKFMFCVTFELPWDVAFKKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIY6</accession>
    <name>A0A1A0HIY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_34546</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatase 2A regulatory subunit.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714328.1">
        <property type="nucleotide sequence ID" value="XM_018856485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIY6"/>
    <dbReference type="STRING" id="869754.A0A1A0HIY6"/>
    <dbReference type="GeneID" id="30029461"/>
    <dbReference type="OrthoDB" id="10264446at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032186">
        <property type="term" value="P:cellular bud neck septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070199">
        <property type="term" value="P:establishment of protein localization to chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051754">
        <property type="term" value="P:meiotic sister chromatid cohesion, centromeric"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031107">
        <property type="term" value="P:septin ring disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031134">
        <property type="term" value="P:sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000372">
        <property type="entry name" value="Serine/threonine protein phosphatase 2A regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE 2A PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10257:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A 56 KDA REGULATORY SUBUNIT GAMMA ISOFORM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028043">
        <property type="entry name" value="PP2A_B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="39"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR028043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="745" mass="84137" checksum="6406ED0739C90F65" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ38</accession>
    <name>A0A1A0HJ38_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714497.1">
        <property type="nucleotide sequence ID" value="XM_018855625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ38"/>
    <dbReference type="GeneID" id="30028601"/>
    <dbReference type="OrthoDB" id="4083656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="43157" checksum="3B959459DC4EC07B" modified="2016-10-05" version="1">MISHMMVFSRVAGLGPGPLVARTLPRPSTMRAFSTVLPRRNSKLPESFDPKKNLPSQGEWKHLKQHIPGEAEQTNTVRDRIPMFPLGKENVPTLLPRPGVPRVGPNMPFSRVIQILKSKVNPELIYESEPHRLYFLGCFCCAVMFTIYGCVLLEYAWFNANKKWDENEQELALPFRRRQFGIDLLTYGSLGAVALFAAYHFATFPTRLMRRIWYLPGPVEHIKFTSYPLIPGRPTPMYTVPLEDLARRDKARVWTGKGFYGTADKSLFFFVLKEKSTNKNWIVDRKGFFWSDGRVFDLLFGKETLAEAEAGIPYDEQIGIIHREVKKKKKELRAKHGIFYQFKLGAQEAQKDLKSATSYAKTLAASKKPKGLPKDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ40</accession>
    <name>A0A1A0HJ40_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Cysteine dioxygenase</fullName>
            <ecNumber evidence="2 9">1.13.11.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_76926</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-cysteine + O2 = 3-sulfino-L-alanine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20441"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:61085"/>
            <dbReference type="EC" id="1.13.11.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="9">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="2 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714511.1">
        <property type="nucleotide sequence ID" value="XM_018858607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ40"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ40"/>
    <dbReference type="GeneID" id="30031583"/>
    <dbReference type="OrthoDB" id="543511at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019448">
        <property type="term" value="P:L-cysteine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10548">
        <property type="entry name" value="cupin_CDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="CYSTEINE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918:SF1">
        <property type="entry name" value="CYSTEINE DIOXYGENASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0883">Thioether bond</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="7">
        <location>
            <begin position="138"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="26827" checksum="93B2D5C5BC802C0A" modified="2016-10-05" version="1">MLSVNSAGSSDMKSSMMCTAPSAPQSREATVPTFGCLEDETNNDLKLDNKFGQLIDSLRAVLGTKGLACADVDVGEIRRLMERYQSDETEWGKFALSDASKGYTRNGVVNINGNANLLVLVWSPGKGSAIHDHANAHCCMKVLKGLLTESLYDVPETAGHAMVPRQITELRRDEVGYMSDDIGLHKISNTSAEDFAVSLHLYTPPYASLYGCSMYESGTLRKHHVNMSKYYSWQGTVLNQNGGSSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJJ7</accession>
    <name>A0A1A0HJJ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="12">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_10335</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714652.1">
        <property type="nucleotide sequence ID" value="XM_018853742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJJ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJJ7"/>
    <dbReference type="GeneID" id="30026718"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="16">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="74"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="262"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="596"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="10">
        <location>
            <begin position="43"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="675"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="705"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="725"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA24171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="762" mass="85972" checksum="3724AC17571EBF10" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK74</accession>
    <name>A0A1A0HK74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_34241</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714684.1">
        <property type="nucleotide sequence ID" value="XM_018856452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK74"/>
    <dbReference type="STRING" id="869754.A0A1A0HK74"/>
    <dbReference type="GeneID" id="30029428"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033551">
        <property type="term" value="C:monopolin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000370">
        <property type="term" value="P:positive regulation of clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000785">
        <property type="term" value="P:regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001159">
        <property type="term" value="P:regulation of protein localization by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106214">
        <property type="term" value="P:regulation of vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051455">
        <property type="term" value="P:spindle attachment to meiosis I kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14125">
        <property type="entry name" value="STKc_CK1_delta_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000159">
        <property type="entry name" value="Casein kinase I hhp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="9"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="301"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="310"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="417" mass="48033" checksum="A2AC988AE0685B02" modified="2016-10-05" version="1">MDLRVGKKYRLGKKIGSGSFGDIYLGTNVISGEEIAIKLENVKAKHPQLEYEAKVYKALSGGVGIPFVRWYGTECDYNAMVIDLLGPSLEDLFNYCNRKFTYKTVLLLADQLICRIEYIHARCFIHRDIKPDNFLMGTGRRGSQVNVIDFGLAKKYRDPRTHAHIPYRENKNLTGTARYASVNTHLGIEQSRRDDLESLGYVLIYFCRGSLPWQGLKAATKRQKYDKIMEKKMTIPNDVLTKGLPDKFLDYMNYVKNLRFEDKPNYAYLRNLFRDLFKRENYRYDYVFDWTLYKFQQDKQKEEGKGGEGQAEQSQGHQQIQQQQIQQQQIQQMQLQHQQQSQIHASPPKMSSEMNSAVQQMTVLAGPGHPDKAGVHLASQVAQLGSGRFADATFHQSYDHEGSPQRGHRPSNNQAWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKK1</accession>
    <name>A0A1A0HKK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">Alpha-1,3/1,6-mannosyltransferase ALG2</fullName>
            <ecNumber evidence="6 16">2.4.1.132</ecNumber>
            <ecNumber evidence="5 16">2.4.1.257</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_34126</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 16">Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>a beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29515"/>
            <dbReference type="Rhea" id="RHEA-COMP:19511"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58472"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="EC" id="2.4.1.132"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14 16">
            <dbReference type="Rhea" id="RHEA:29516"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>an alpha-D-Man-(1-&gt;3)-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP-alpha-D-mannose = an alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29519"/>
            <dbReference type="Rhea" id="RHEA-COMP:19513"/>
            <dbReference type="Rhea" id="RHEA-COMP:19515"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:132510"/>
            <dbReference type="ChEBI" id="CHEBI:132511"/>
            <dbReference type="EC" id="2.4.1.257"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15 16">
            <dbReference type="Rhea" id="RHEA:29520"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 16">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.132" evidence="6 16"/>
    <dbReference type="EC" id="2.4.1.257" evidence="5 16"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715036.1">
        <property type="nucleotide sequence ID" value="XM_018856443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HKK1"/>
    <dbReference type="GeneID" id="30029419"/>
    <dbReference type="OrthoDB" id="448893at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004378">
        <property type="term" value="F:GDP-Man:Man(1)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102704">
        <property type="term" value="F:GDP-Man:Man(2)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033164">
        <property type="term" value="F:initiation-specific glycolipid 1,6-alpha-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03805">
        <property type="entry name" value="GT4_ALG2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027054">
        <property type="entry name" value="ALG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918">
        <property type="entry name" value="ALPHA-1,3/1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45918:SF1">
        <property type="entry name" value="ALPHA-1,3_1,6-MANNOSYLTRANSFERASE ALG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 16 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="18">
        <location>
            <begin position="13"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="17">
        <location>
            <begin position="213"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA24555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="462" mass="51972" checksum="D43A028039BBE047" modified="2016-10-05" version="1">MSKIAFIHPDLGIGGAERLVVDAAVGLQDLGHTIKVYTSHCDLSHCFEEVNDGTLDVSVYGDFLPSSILSKFHILFAILRQLYLVLWLIISGEIGQYDYFIVDQLSMGVPLLSIFSKSSARVLFYCHFPDQLLSKKGGFLKRLYRMPFDYLEEWSTGVSDTIVVNSTFTKSIFHETFTNLSSTDPGVIYPCVNVQDISTSNTNGEAELLSFMNGNKYFLSINRFERLKNIGLAIKSFAKLLKVVPPSQAPKLILAGGFDSRVKENVEYLSELQTMCDSLELTHYTFRGKLVLMPRSTQVIFLPSVNSAIKNAALKHAELLLYTPTFEHFGIVPVESMLNKTPVLAINKGGPLESIVNFNDKNLNVATGFNRPNDIDEWASVLVDYYTEFEQQTKTRLGENGAKRAISMFSREEMSRAFETRLNASKDTVSRKGHLYNALTLWKFWLLAMFFASCAIFFQSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKW2</accession>
    <name>A0A1A0HKW2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_30775</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715013.1">
        <property type="nucleotide sequence ID" value="XM_018855704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKW2"/>
    <dbReference type="STRING" id="869754.A0A1A0HKW2"/>
    <dbReference type="GeneID" id="30028680"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000001">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF26">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="436"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="8">
        <location>
            <begin position="46"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="513" mass="56394" checksum="122E2F4A3F562CA3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ32</accession>
    <name>A0A1A0GZ32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Translation factor GUF1, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_80196</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Mitochondrion inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GTP-binding elongation factor family. LepA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709331.1">
        <property type="nucleotide sequence ID" value="XM_018859192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ32"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ32"/>
    <dbReference type="GeneID" id="30032168"/>
    <dbReference type="OrthoDB" id="1074at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16260">
        <property type="entry name" value="EF4_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01890">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000078">
        <property type="entry name" value="Elongation factor 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000015">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000007">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2570:FF:000001">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000004">
        <property type="entry name" value="Translation factor GUF1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="entry name" value="Elongation factor 4, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512:SF7">
        <property type="entry name" value="TRANSLATION FACTOR GUF1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="TRANSLATION FACTOR GUF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="57"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="66"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="133"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="187"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA17034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="658" mass="73694" checksum="50183085DFFF582F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Q6</accession>
    <name>A0A1A0H1Q6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">LisH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709356.1">
        <property type="nucleotide sequence ID" value="XM_018854441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Q6"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Q6"/>
    <dbReference type="GeneID" id="30027417"/>
    <dbReference type="OrthoDB" id="4097090at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012791545" description="LisH domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="330"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA17961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="626" mass="67595" checksum="8F390E67AE9B8056" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1Z7</accession>
    <name>A0A1A0H1Z7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metacaspase-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_47781</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in cell death (apoptosis).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C14B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709342.1">
        <property type="nucleotide sequence ID" value="XM_018858219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1Z7"/>
    <dbReference type="STRING" id="869754.A0A1A0H1Z7"/>
    <dbReference type="GeneID" id="30031195"/>
    <dbReference type="OrthoDB" id="3223806at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050452">
        <property type="entry name" value="Metacaspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104:SF30">
        <property type="entry name" value="METACASPASE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48104">
        <property type="entry name" value="METACASPASE-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Peptidase C14 caspase" evidence="8">
        <location>
            <begin position="121"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="82"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA17947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="416" mass="45062" checksum="54E81620145B05D9" modified="2016-10-05" version="1">MFPGSGGHSYNNSQGHYQGGYPNQQSNYGYQGPPPSNPNGPPPGNYYGGPPGPPQNGYYGRPPPGPPHGPPPHSQWIPQGGNLNSSLNNSSHAPTSAPPSAPQSFGQSSNMTFQYSNCTGKKKALLIGINYYGSKNELRGCVNDVKNMSKFLNQYYGYSYDDMVILTDDQREIARLPTRDNIIRAMQWLTKNAAPNDSYFFHISSHGGLVPDQNGDEESGFDSCVYPLDFETAGPIIDDEMHDLMVKPLPPGCRLVALYDACHSGTALDLPFVYSTKGVVKEPNLWKDAGSGALKAFMNYEAGNISGAMSAVSGIFKKVTNYQSTDRDKIVQSKMSSADVISLSGCKDDQTSADAVEAGQSTGAMSWAFISVLTQSRNQSYLSLLNNMRSILSAKYSQKPQLSASHPQDMNLAFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H202</accession>
    <name>A0A1A0H202_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">METBIDRAFT_47645</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709347.1">
        <property type="nucleotide sequence ID" value="XM_018858201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H202"/>
    <dbReference type="STRING" id="869754.A0A1A0H202"/>
    <dbReference type="GeneID" id="30031177"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904161">
        <property type="term" value="P:DNA synthesis involved in UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903459">
        <property type="term" value="P:mitotic DNA replication lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006278">
        <property type="term" value="P:RNA-templated DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.730:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000351">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.730">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025687">
        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 20" id="KW-0408">Iron</keyword>
    <keyword evidence="16 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="22">
        <location>
            <begin position="209"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="21">
        <location>
            <begin position="506"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="23">
        <location>
            <begin position="973"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OBA17952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1067" mass="121291" checksum="1891DC9D347B571D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4G2</accession>
    <name>A0A1A0H4G2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709499.1">
        <property type="nucleotide sequence ID" value="XM_018858193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4G2"/>
    <dbReference type="STRING" id="869754.A0A1A0H4G2"/>
    <dbReference type="GeneID" id="30031169"/>
    <dbReference type="OrthoDB" id="110024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="272" mass="31717" checksum="EFB870D82F824534" modified="2016-10-05" version="1">MTMEFDLYKTLCVSTTASPVEIKKAYKKLSLQYHPDKIQQLETNEDKDYFPKIQLAYSVLSDPQKRKIYDTTGSYGNSSDFSAGDFDWKEYFQNMTEKITIDMIDEDREKYQKSLEEKDDILHNFVYYEGDFLRLFEVIPHLEFDEALENRVFDMIDKAVSNDEIAMDKTIQLTWNKYKKSRKTKVKQVLKKMAKEAQEAAELAKIIGQKKASGENDLKAMIQKNSSGRMDDLILSLEAKYGRSKGKKRSVPDDEEFAKIQAQLTKGKKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4S9</accession>
    <name>A0A1A0H4S9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_28566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709490.1">
        <property type="nucleotide sequence ID" value="XM_018855358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4S9"/>
    <dbReference type="GeneID" id="30028334"/>
    <dbReference type="OrthoDB" id="3784821at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="114" mass="13271" checksum="1988AF11A9D331A2" modified="2016-10-05" version="1" fragment="single">AKKSAFFEEMVETGRPNWKKAPPEVKARSRGVFLILLSIPVILVPGWEIYRRLSGRSTKKVQVGELGDDKSIRKFDEIEKWETERDSILYKIFGRDFYLDGFTAKTMKQDEPTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H532</accession>
    <name>A0A1A0H532_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709678.1">
        <property type="nucleotide sequence ID" value="XM_018854367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H532"/>
    <dbReference type="GeneID" id="30027343"/>
    <dbReference type="OrthoDB" id="4087712at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="432"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="593"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="660"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="688"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="730" mass="78432" checksum="145A57FE51873E5E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H561</accession>
    <name>A0A1A0H561_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="12">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_47452</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709625.1">
        <property type="nucleotide sequence ID" value="XM_018858166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H561"/>
    <dbReference type="STRING" id="869754.A0A1A0H561"/>
    <dbReference type="GeneID" id="30031142"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF134">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="325" mass="37121" checksum="61B517CB0DFEC773" modified="2016-10-05" version="1">MYSVETPFLIELWPLFDAAVTKASGGRFVPSQFEFVPHQTAFSSFPVVIGAIAVYYTVIFGGDYIFKKFKIKPFVLNGLFQIHNLFLTTLSLTLLVLMCEELIPMIYKYGLFYAICNRNAWTQKLVTLYYLNYLTKFCEFIDTVFLVVKQKKLTFLHTYHHGATALLCYTQLIGLTPISWVPITLNLFVHVVMYWYYFLAARGIRVWWKEWVTRLQIIQFVLDLGFVYFATYQKIAHVYLSKLGDFPYCGDCAGTMLAAFSGCGILSSYLVLFIAFYIHVYKRKASKKAKEVKAVPGGVAAQVNAYVHASGKSNSPAPEGLRKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5A4</accession>
    <name>A0A1A0H5A4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_18137</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709796.1">
        <property type="nucleotide sequence ID" value="XM_018854771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5A4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5A4"/>
    <dbReference type="GeneID" id="30027747"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000312">
        <property type="entry name" value="Ran binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="51"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="21184" checksum="5F37171746BDBF7D" modified="2016-10-05" version="1" fragment="single">PKPPTSNVFLMFGAKKEKKEDKEDKEEGKENDETEKKDNAEGDAEEEVDVHFEPLVHLEKVDVKTNEEDEEVMFKVRAKLFRFHADTKEWKERGTGDVKFLKHKETKKTRVLMRRDKTLKICANHLVQGDYELKPNIGSDRSWVYTVTADISEGEPEAQTLAIRFGNKENADKFKEHFDEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5D4</accession>
    <name>A0A1A0H5D4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7 11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_79823</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709826.1">
        <property type="nucleotide sequence ID" value="XM_018859133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5D4"/>
    <dbReference type="STRING" id="869754.A0A1A0H5D4"/>
    <dbReference type="GeneID" id="30032109"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="12">
        <location>
            <begin position="4"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 10">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="9 10">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="306" mass="34955" checksum="55DD248021591945" modified="2016-10-05" version="1">MSSGWNTIVSDAGVFTELVSRLGVKDVQIEELYSIDSASLKALAPVHAVVFLFKYGKVDRDSTMHDVPLDGVYDEDYQDKGIFFARQTIQNACATQAVLNSLLNKTSELDIGTELSNIHGFVAGFDPDMCGDTISNSEVIREVHNSFSAPKFIELDDSARPEVDEKNNGLFHFITYLNINNTIYELDGLKRSPIKHDNLLSPDEFYDKLPEVLHRRISKYSDEIRFSLLAITNDKLQQYQALGDELSTAQELDKRQRWARDNQWRRHDYTRLTVELLKDISSSLPDNEWEEVLRKAKLKTMNKSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5L0</accession>
    <name>A0A1A0H5L0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Trimethyllysine dioxygenase</fullName>
            <ecNumber evidence="5">1.14.11.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Epsilon-trimethyllysine 2-oxoglutarate dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">TML hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">TML-alpha-ketoglutarate dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_46766</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6),N(6),N(6)-trimethyl-L-lysine + 2-oxoglutarate + O2 = (3S)-3-hydroxy-N(6),N(6),N(6)-trimethyl-L-lysine + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:14181"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:58100"/>
            <dbReference type="ChEBI" id="CHEBI:141499"/>
            <dbReference type="EC" id="1.14.11.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; carnitine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-BBH/TMLD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.8" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709767.1">
        <property type="nucleotide sequence ID" value="XM_018858073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5L0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5L0"/>
    <dbReference type="GeneID" id="30031049"/>
    <dbReference type="OrthoDB" id="408743at2759"/>
    <dbReference type="UniPathway" id="UPA00118"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050353">
        <property type="term" value="F:trimethyllysine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045329">
        <property type="term" value="P:carnitine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00250">
        <property type="entry name" value="CAS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2020.30:FF:000002">
        <property type="entry name" value="Putative gamma-butyrobetaine dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.130.10:FF:000001">
        <property type="entry name" value="Trimethyllysine dioxygenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050411">
        <property type="entry name" value="AlphaKG_dependent_hydroxylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012776">
        <property type="entry name" value="Trimethyllysine_dOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02410">
        <property type="entry name" value="carnitine_TMLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696">
        <property type="entry name" value="GAMMA-BUTYROBETAINE HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10696:SF51">
        <property type="entry name" value="TRIMETHYLLYSINE DIOXYGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0124">Carnitine biosynthesis</keyword>
    <keyword evidence="8 19" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-butyrobetaine hydroxylase-like N-terminal" evidence="18">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="TauD/TfdA-like" evidence="17">
        <location>
            <begin position="136"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA19235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="388" mass="44860" checksum="7BBBB9628883D193" modified="2016-10-05" version="1">MSSTELSIEIDGVPTLFHFIWLRDHCHCSACFFDTTKQRLLNSATIDENIHPAKQEYDGKNLSIVWSQDNHLSEYPVDWLRVHAYNPPLIAGDLKVAGEKEILERTLWTTSEIKDVMPVVDFPKIMASSDETDGEEAIKEWCLKLWKYGFTLIDNVPVTPEDTEKLCEKLMYIRPTHYGGFWDFTSDLGKKDTAYTNFDISSHTDGTYWSDTPGLQLFHLLYHDGTGGTTSLVDAFQCAEKMKKLHPESYDLLTKIPIRAHSAGEEKVCIQPDIPQPIFKLDLEGNLIQVRWNQSDRSCMDIWENPADVPKFYQAIRQWYKIISSPENEIFYQLKPGQCLIFDNWRCFHLRTEFTGKRRLCGAYINRDDFVSRLKLLNLGRKAVLDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5M3</accession>
    <name>A0A1A0H5M3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709750.1">
        <property type="nucleotide sequence ID" value="XM_018856293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5M3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5M3"/>
    <dbReference type="GeneID" id="30029269"/>
    <dbReference type="OrthoDB" id="3034343at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="37346" checksum="B76B9F47549D1515" modified="2016-10-05" version="1">MKVTKKILQTEGNYVRPYRSRSERPCDFCRTRKTCCIIENLIPCMACTKFNDGQCTFLQKPLKRNRENTIAIRKPPKQIRSKAGDNIDVLQHTPLLRFTSSLPADSSTDSQLGFDEIHELLSTKSLLWEKAERCARSSSTGNAHSSFYNILQNLLDILPQHPPDASLAPNSYKHLPGITKEDIFCAIQESNDIVTLPETRLHYQSTQSQPRDYKYLENPSSNNPQHCNPHQLEQSEFSSETPTLLDNSVFSLLALGSNSGSRAWIPQAFNPTYALVDSGNTESYGARCFSVNQLQDLLVAASYPTQFHQEYCERFTDGAGDVSGISTLNSFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H607</accession>
    <name>A0A1A0H607_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_33557</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709922.1">
        <property type="nucleotide sequence ID" value="XM_018856330.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H607"/>
    <dbReference type="GeneID" id="30029306"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03369">
        <property type="entry name" value="ABCC_NFT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000825">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF353">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN_PERMEASE VMR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="182"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="361"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="462"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="568"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="988"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1157"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1180"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1264"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="329"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="666"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="1060"/>
            <end position="1319"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1356"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="913"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="425"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="913"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1629" mass="182549" checksum="3358CF87B4A9915F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H663</accession>
    <name>A0A1A0H663_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of fatty acids protein</fullName>
            <ecNumber evidence="10">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_33562</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>an acyl-CoA + malonyl-CoA + H(+) = a 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:50252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:50253"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709929.1">
        <property type="nucleotide sequence ID" value="XM_018856331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H663"/>
    <dbReference type="STRING" id="869754.A0A1A0H663"/>
    <dbReference type="GeneID" id="30029307"/>
    <dbReference type="OrthoDB" id="10259681at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF169">
        <property type="entry name" value="ELONGATION OF FATTY ACIDS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="330" mass="37470" checksum="D6D0043DA1AEC0EE" modified="2016-10-05" version="1">MSFSFGLPDASLWALPKFDTQVPPSPFQCEKLTKIFAASMKVSTPLTIAIVYFTSVHFINPIVVRRQLKKANVSQKDGAKLTEKEMKRLKPAPYKIATYGMFKLFVLLHNIFLCAYSVWTFVGMLNSFSRTVSQIQLKTNTDSTLVNFVHAVCDLENGIFQQTTEDHNLALFGWWFYISKFYEVIDTIIILLKGRPSSLLQSYHHAGAMMCMWSGIRYQSPPIWIFVVFNSFIHSLMYFYFTLACLKIRVPTIVKRALTSMQISQFVLGGSLAVFHAFIWYSDVTGLEPSGCINTSAKALPLLINVAYLTPLTALFAAFYIESYIKRKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H670</accession>
    <name>A0A1A0H670_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_13283</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710054.1">
        <property type="nucleotide sequence ID" value="XM_018854333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H670"/>
    <dbReference type="STRING" id="869754.A0A1A0H670"/>
    <dbReference type="GeneID" id="30027309"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF54">
        <property type="entry name" value="PROTEIN ESCARGOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="222"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="250"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="149"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="360" mass="40804" checksum="7503AED0B0734B71" modified="2016-10-05" version="1">MSRSYQPMPEVPYQSHMPVYYPQTYQQAPPRMPAMEYHYNPELSYPQVAPAPVNWQVFPRQPSQRNDELVSPLANTMKFQHPVQGQTMPMQQARIQYSAVPYMMQPSSTGIQVPRSADAPNRGELRADQGPPRAGMVVNMMKPLNAAALDASSSESSGTSKSLPVSLSSSISPNSAAHKNGKGAHSHTSDPSANVCEVCGKVFQKPYNLKSHMKTHSTERPFKCLFCPKTFARSHDRKRHENLHGGQKNFKCEGYLRNGVTRWGCGKKFARSDALSRHFRTETGYLCIKQFMDEEKEREACEEHRQALQYPHMHPAPPFYHQQQPQALTNQTYSVAPGMRPYDQMSHMQPQILLPPLLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6B2</accession>
    <name>A0A1A0H6B2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATPase AAA-type core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709981.1">
        <property type="nucleotide sequence ID" value="XM_018857944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6B2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6B2"/>
    <dbReference type="GeneID" id="30030920"/>
    <dbReference type="OrthoDB" id="10064318at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="724"/>
            <end position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="816" mass="92341" checksum="31D5AA0C8CD6267B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Y0</accession>
    <name>A0A1A0H6Y0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histone deacetylase interacting domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_13404</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710191.1">
        <property type="nucleotide sequence ID" value="XM_018854354.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H6Y0"/>
    <dbReference type="GeneID" id="30027330"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000001">
        <property type="entry name" value="Paired amphipathic helix protein Sin3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000002">
        <property type="entry name" value="Paired amphipathic helix protein SIN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1160.11:FF:000003">
        <property type="entry name" value="Paired amphipathic helix SIN3-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346:SF0">
        <property type="entry name" value="SIN3A, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="9">
        <location>
            <begin position="640"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="106"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="421"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="499"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="934"/>
            <end position="1007"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="1418"/>
            <end position="1443"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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            <begin position="116"/>
            <end position="129"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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            <begin position="303"/>
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            <begin position="434"/>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1443" mass="163453" checksum="C84772A031EA1829" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H774</accession>
    <name>A0A1A0H774_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">rRNA biogenesis protein RRP5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Ribosomal RNA-processing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_32824</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of rRNA. Required for the formation of 18S and 5.8S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018856135.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034512">
        <property type="term" value="F:box C/D sno(s)RNA binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:box H/ACA snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0008266">
        <property type="term" value="F:poly(U) RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034463">
        <property type="term" value="P:90S preribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000464">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="protein RRP5 homolog"/>
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        <property type="entry name" value="rRNA biogenesis protein RRP5"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="9"/>
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        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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    <dbReference type="InterPro" id="IPR045209">
        <property type="entry name" value="Rrp5"/>
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        <property type="entry name" value="Rrp5_S1_rpt_hs1_sc1"/>
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        <property type="entry name" value="S1_domain"/>
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        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 11 PRE-RRNA PROCESSING PROTEIN RRP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23270:SF10">
        <property type="entry name" value="PROTEIN RRP5 HOMOLOG"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="13"/>
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        <property type="match status" value="12"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
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        <location>
            <begin position="99"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="305"/>
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    <feature type="domain" description="S1 motif" evidence="11">
        <location>
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            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="574"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="663"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="758"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="857"/>
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        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
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        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
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    <feature type="domain" description="S1 motif" evidence="11">
        <location>
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        <location>
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        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
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            <end position="1398"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1684" mass="185973" checksum="6B157EB8320D8613" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7A3</accession>
    <name>A0A1A0H7A3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_26547</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710431.1">
        <property type="nucleotide sequence ID" value="XM_018855319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7A3"/>
    <dbReference type="GeneID" id="30028295"/>
    <dbReference type="OrthoDB" id="4014468at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="196" mass="22057" checksum="72A2FA25443AFE2F" modified="2016-10-05" version="1" fragment="single">VLEELCDSPEFEAVLRVRTERVQAFLGSKLPLTFHELLSILCGLSNMNIKTSPLPTGSLFGDVFTQQDALRQLGARFFDFHCANTLVFGDLHYLSCTAEELEQSISIFQDKESLIMKLMNASDLSECVIPYRGVHSAGLIKNRATGFRQRIKSRTCVASFYSMLGILVRKFGSEAVVRDFWNPKMLHPIHGLLRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D0</accession>
    <name>A0A1A0H7D0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_12919</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DEAH subfamily. DDX15/PRP43 sub-subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710410.1">
        <property type="nucleotide sequence ID" value="XM_018854280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7D0"/>
    <dbReference type="GeneID" id="30027256"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022613">
        <property type="term" value="P:ribonucleoprotein complex biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17973">
        <property type="entry name" value="DEXHc_DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000007">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000324">
        <property type="entry name" value="pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000003">
        <property type="entry name" value="Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044756">
        <property type="entry name" value="DHX15_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF109">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="103"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="293"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="757" mass="86089" checksum="3034DAAF66491381" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7F7</accession>
    <name>A0A1A0H7F7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_33018</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710554.1">
        <property type="nucleotide sequence ID" value="XM_018856192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7F7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7F7"/>
    <dbReference type="GeneID" id="30029168"/>
    <dbReference type="OrthoDB" id="4151048at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052202">
        <property type="entry name" value="Yeast_MetPath_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782:SF1">
        <property type="entry name" value="PYRIMIDINE PATHWAY REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47782">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="83"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1024" mass="117017" checksum="D4CC147E5BD28A29" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Q3</accession>
    <name>A0A1A0H7Q3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">triacylglycerol lipase</fullName>
            <ecNumber evidence="1">3.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_32946</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710447.1">
        <property type="nucleotide sequence ID" value="XM_018856170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Q3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Q3"/>
    <dbReference type="GeneID" id="30029146"/>
    <dbReference type="OrthoDB" id="406844at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00519">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051299">
        <property type="entry name" value="AB_hydrolase_lip/est"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640:SF3">
        <property type="entry name" value="LIPASE LIH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46640">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G06510)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="3">
        <location>
            <begin position="2"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="158" mass="17962" checksum="8E6356901AFCDB90" modified="2016-10-05" version="1">MDKYPNYRLSVTGHSLGAALAILAGIELRLMGLDVLVVTLATPRIGDKNFASYVDDLFNTKDVTQVIDKEKSFDNIGTALIRMIHLHDVVPYLPPTRYFRHSGYEYFLAAEGVSQTPQTIKRQGTTYLEDEPQNYLEVLPNFRRLDHANYFLPVSHCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7V5</accession>
    <name>A0A1A0H7V5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_44872</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSP2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710631.1">
        <property type="nucleotide sequence ID" value="XM_018857846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7V5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7V5"/>
    <dbReference type="GeneID" id="30030822"/>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF36">
        <property type="entry name" value="MEMBRANE-ANCHORED LIPID-BINDING PROTEIN LAM4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="665"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="436"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="600"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="307"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="778" mass="87047" checksum="FD237E6DCD5A8752" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8B3</accession>
    <name>A0A1A0H8B3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CIA30-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_43637</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CIA30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710742.1">
        <property type="nucleotide sequence ID" value="XM_018857682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8B3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8B3"/>
    <dbReference type="GeneID" id="30030658"/>
    <dbReference type="OrthoDB" id="42561at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010257">
        <property type="term" value="P:NADH dehydrogenase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039131">
        <property type="entry name" value="NDUFAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194:SF18">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="6">
        <location>
            <begin position="37"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="229"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="266" mass="30222" checksum="8F6B415648C77EA1" modified="2016-10-05" version="1">MSMRSLGLNSALGRMFTKSTDQTKALQTVLNFRKHPETSLEKIMTRSDKEMGGYSEVHMDIDPQTGAGHFHGYLNLDLPKDNPEITRSGYAMFRTKDQRDSWISGDSHWDWSHYALMVLRVKGDRRKYLVNIQANTPLVTDLFQHRLFLHHPGEWETVVIPLDDFVMTNWGVIQDGSELNKAEVKTVGIGLLDKQYGPFSLKIDWIKVMTGTEVEKVAKKSRAERLALEHGAKHDGHKDVESNMPTGDGCSALNTHKIDEPKETIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8B8</accession>
    <name>A0A1A0H8B8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_193002</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710747.1">
        <property type="nucleotide sequence ID" value="XM_018854856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8B8"/>
    <dbReference type="GeneID" id="30027832"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16913" checksum="0D752C14100B22B1" modified="2016-10-05" version="1">MPPKKCFWNFPEGIPESGSQLAHSSRRVTAARSRRSTGTRFRPSYHWETRDEDVYESSEQGTSRGTIAFCSRNRVTDTASIFTRNPGIVSHFSRQRKLIVRLRRPCGGLPARVRLQYHTRNSESTHCGSHHAIASGSIYNFPMCGRAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8H2</accession>
    <name>A0A1A0H8H2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_198579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710936.1">
        <property type="nucleotide sequence ID" value="XM_018854901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8H2"/>
    <dbReference type="GeneID" id="30027877"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8953" checksum="6C01772A68A332EB" modified="2016-10-05" version="1">MHNIAALPLIIYCIFKNNKQTDDIRKENKSINETAKSHNRLHATVIFLQLCRPRDRNLASHREKMPDNTTSQSEPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U2</accession>
    <name>A0A1A0H8U2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SAGA-associated factor 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_43898</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions as component of the transcription regulatory histone acetylation (HAT) complex SAGA. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction and promoter selectivity, interaction with transcription activators, and chromatin modification through histone acetylation and deubiquitination. SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). Involved in transcriptional regulation of a subset of SAGA-regulated genes. Within the SAGA complex, participates in a subcomplex, that specifically deubiquitinates histones H2B.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 1.8 MDa SAGA transcription coactivator-HAT complex. SAGA is built of 5 distinct domains with specialized functions. Within the SAGA complex, SUS1, SGF11, SGF73 and UBP8 form an additional subcomplex of SAGA called the DUB module (deubiquitination module). Interacts directly with SGF73, SUS1 and UBP8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the SGF11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711061.1">
        <property type="nucleotide sequence ID" value="XM_018857714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8U2"/>
    <dbReference type="GeneID" id="30030690"/>
    <dbReference type="OrthoDB" id="21557at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070461">
        <property type="term" value="C:SAGA-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013246">
        <property type="entry name" value="SAGA_su_Sgf11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08209">
        <property type="entry name" value="Sgf11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU261113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="88" mass="10083" checksum="689DE7C61F0943D5" modified="2016-10-05" version="1" fragment="single">DIVTGIIRQISVNCISRHRALQQTIGKENQPFTSLYLESCTKDIFGQEKSKLKGLETSRYFECGNCGRKISGGRFALHINKCFDRDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H905</accession>
    <name>A0A1A0H905_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_43529</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710885.1">
        <property type="nucleotide sequence ID" value="XM_018857670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H905"/>
    <dbReference type="STRING" id="869754.A0A1A0H905"/>
    <dbReference type="GeneID" id="30030646"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="187" mass="20696" checksum="0601EB6C54DA6A31" modified="2016-10-05" version="1">MIIYSLYILNKAGGLIYQNDLNPGLARLNANDYLVLAGTLHGVHAIATRIRPVDNTIPQDEVPSANTSILTSGKAKLPNTNSLGLQHMDTDLFSLYVFQTLTGLKFVIITSPGKSGQQNNQTRGGSSSTSKGDLTRQFQSVGAVYKEIYQFYCDYVMKNPFYALYMPIKCAMFEQKVRAMANIRITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H968</accession>
    <name>A0A1A0H968_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_198619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710945.1">
        <property type="nucleotide sequence ID" value="XM_018854902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H968"/>
    <dbReference type="GeneID" id="30027878"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="138" mass="14840" checksum="857A1715F0FF038D" modified="2016-10-05" version="1">MHVSSVPRRSLLVSPWPALAQYQLLELSGLWLGTPLFFVSSDLSASLVTSARPCGSSFLLCRSSLFFFFLGLNIVIPFPSAWLCLFAGLVVVVQASGAARPEHAAPPNLNFGGRMNLWVPRTQGTNGAPKPSPGMGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9C1</accession>
    <name>A0A1A0H9C1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_22916</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710995.1">
        <property type="nucleotide sequence ID" value="XM_018855220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9C1"/>
    <dbReference type="GeneID" id="30028196"/>
    <dbReference type="OrthoDB" id="4008151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024460">
        <property type="entry name" value="Protamine-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06382">
        <property type="entry name" value="Protamine_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="233" mass="27678" checksum="3557B3DED6026884" modified="2016-10-05" version="1" fragment="single">SSSVIELKTRPDLYNGVFTKFGWVLVKKTHVVRGWTQLQLNQVLEEKLGEPLDQHDWDTVQQYADEWVRTANANQVPKLKRRTMADKSREEDMKLYLQQLRYDETEEISADDEKRRKRRLDGTKEIKQHKEINKNAVSFTNAWNYYMAVTYKTHENLSPKERRVKVGTDWRQMSMDQKEVYRQDYTRLLNLGKDVLHGEIVDQEVKLKATQKLWEAKERARLRKLGVLSPANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9K3</accession>
    <name>A0A1A0H9K3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_25094</name>
        <name evidence="2" type="ORF">METBIDRAFT_28223</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711214.1">
        <property type="nucleotide sequence ID" value="XM_018855350.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711218.1">
        <property type="nucleotide sequence ID" value="XM_018855286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9K3"/>
    <dbReference type="GeneID" id="30028262"/>
    <dbReference type="GeneID" id="30028326"/>
    <dbReference type="OrthoDB" id="4089884at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8421" checksum="862E9F98EE3B0143" modified="2016-10-05" version="1" fragment="single">SHRAYSVKSKAKDFNKLLDQIGDEKTLASQNTLKYVRRVGAGSIIIVICSLFWASSLTAPVKESPVDVGNLAQDVRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9M1</accession>
    <name>A0A1A0H9M1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein 60S</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_203440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711233.1">
        <property type="nucleotide sequence ID" value="XM_018854963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9M1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9M1"/>
    <dbReference type="GeneID" id="30027939"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05831">
        <property type="entry name" value="Ribosomal_P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="105" mass="10975" checksum="06F6613A59CE550B" modified="2016-10-05" version="1">MSTESALSYAALILADADVEITSDKLSTLVTEAKVEFEPIWADLFAKALEGKNLKDFFFNFSAAPAASAGGAAAVSGGAEEAAAEEKVEEAKEESDDDMGFGLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA08</accession>
    <name>A0A1A0HA08_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PLP-dependent transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711230.1">
        <property type="nucleotide sequence ID" value="XM_018856108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA08"/>
    <dbReference type="STRING" id="869754.A0A1A0HA08"/>
    <dbReference type="GeneID" id="30029084"/>
    <dbReference type="OrthoDB" id="5978656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092:SF2">
        <property type="entry name" value="HERCYNYLCYSTEINE SULFOXIDE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43092">
        <property type="entry name" value="L-CYSTEINE DESULFHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="2">
        <location>
            <begin position="31"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="422" mass="45593" checksum="01CFA485B7692264" modified="2016-10-05" version="1">MTFGHAFKKKHFPASDNGFVPVNHGSFGLPPQCVLDKYIEALMDDVASPDTYIRLTQPAQYAAATRAVAQVLNCAPQNVALVANATSGVNTVLRSLPFKKGDKIAYASTTYGACANTVQFLARAVGIVPVAVPLDFPMLAAAVTAKFEHTFRTHDIKLALFDAVVSMPGVRMPFEAVARACAAHGVLSLVDGAHLIGLIPVDLGGAAFRPDFYVLNLHKWLSLPRGCAVLYVAPQHHRAVQTLPISHSYVDPDAPLSPEAEANLLALKFTFVGSDTFATLACIPTAIAFRARECGGEAAIRTYCEALARQAGALAVRRWPGAWLVENREQSLVTAMVSVVVPVEQYSRAFDAADAAQMKRLVDFVSVHLLENYRTFVPFAAHNGKVVMRLSAQVYNELSDYEYAVAAAQKALRAFFARKSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA24</accession>
    <name>A0A1A0HA24_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DUF221-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_31533</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CSC1 (TC 1.A.17) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711376.1">
        <property type="nucleotide sequence ID" value="XM_018855874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA24"/>
    <dbReference type="STRING" id="869754.A0A1A0HA24"/>
    <dbReference type="GeneID" id="30028850"/>
    <dbReference type="OrthoDB" id="1076608at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022257">
        <property type="entry name" value="PHM7_ext"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF26">
        <property type="entry name" value="DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G10920)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12621">
        <property type="entry name" value="PHM7_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="512"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="586"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="652"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="10">
        <location>
            <begin position="10"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="11">
        <location>
            <begin position="181"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="8">
        <location>
            <begin position="370"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="10TM putative phosphate transporter extracellular tail" evidence="9">
        <location>
            <begin position="763"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="852" mass="96743" checksum="01ACAF95F0BF29FD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA90</accession>
    <name>A0A1A0HA90_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">OPT-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_41894</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711430.1">
        <property type="nucleotide sequence ID" value="XM_018857463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA90"/>
    <dbReference type="GeneID" id="30030439"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="367"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="606"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="634"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="694"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <end position="745"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="757"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="807"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="835"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="901" mass="102408" checksum="B23FA36030A2BD00" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG2</accession>
    <name>A0A1A0HAG2_9ASCO</name>
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        <recommendedName>
            <fullName evidence="5">DUF1531-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018858839.1"/>
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    <dbReference type="STRING" id="869754.A0A1A0HAG2"/>
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    <dbReference type="OrthoDB" id="4227028at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Trafficking_Pga2"/>
    </dbReference>
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        <property type="entry name" value="PROCESSING OF GAS1 AND ALP PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28199:SF1">
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    </dbReference>
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            <end position="40"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="15609" checksum="83BC3B08B035C718" modified="2016-10-05" version="1">METPAAPLGFVYDYVEKQSLQKYLRLVIIVCIYIFARGYYSNWAKQRHIRTQLAIDKREKELDAQRKERDESEKFEELDREATSFGWGKATRRNVKRQEQVLQDAAQQLRERHQGEYDAAEDHDIDDLLDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAJ3</accession>
    <name>A0A1A0HAJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_166181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <consortium name="DOE Joint Genome Institute"/>
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                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
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    </dbReference>
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    <dbReference type="GeneID" id="30027638"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="138" mass="15762" checksum="598112C26830318A" modified="2016-10-05" version="1">MGGVMAGLWVVLWLVYGCCLYVTGLGRRTLKHTHETSHCIAMRIPAIRNNHKNTGSMTACMSAYHMVLVHVYTGYLGTKHWHTNAIHALSTKVQLCKYRQDRAEVLPPEVSGISFAETERLPFVPCSHFWDRWIVDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAW5</accession>
    <name>A0A1A0HAW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_178734</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
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                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="869754.A0A1A0HAW5"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="684" mass="76571" checksum="D9FE1BA070140E8F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB17</accession>
    <name>A0A1A0HB17_9ASCO</name>
    <protein>
        <recommendedName>
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            <ecNumber evidence="3">4.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP pyrophosphate-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Adenylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_78157</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adenylyl cyclase class-3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711593.1">
        <property type="nucleotide sequence ID" value="XM_018858834.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HB17"/>
    <dbReference type="GeneID" id="30031810"/>
    <dbReference type="OrthoDB" id="2021138at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046579">
        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17214">
        <property type="entry name" value="RA_CYR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000220">
        <property type="entry name" value="Adenylate cyclase AcyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048580">
        <property type="entry name" value="CYAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055071">
        <property type="entry name" value="RA_PHLPP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21187">
        <property type="entry name" value="CYAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23010">
        <property type="entry name" value="RA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0115">cAMP biosynthesis</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ras-associating" evidence="15">
        <location>
            <begin position="447"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="PPM-type phosphatase" evidence="16">
        <location>
            <begin position="1158"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="14">
        <location>
            <begin position="1490"/>
            <end position="1627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="190"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="307"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="354"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="84"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="231"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="252"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="366"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA21083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1857" mass="205661" checksum="F4A6831ED3108FC4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB49</accession>
    <name>A0A1A0HB49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF1748-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_41083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711725.1">
        <property type="nucleotide sequence ID" value="XM_018857352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB49"/>
    <dbReference type="STRING" id="869754.A0A1A0HB49"/>
    <dbReference type="GeneID" id="30030328"/>
    <dbReference type="OrthoDB" id="16824at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013726">
        <property type="entry name" value="Mitofissin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28075:SF1">
        <property type="entry name" value="DUF1748-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08520">
        <property type="entry name" value="Mitofissin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="90" mass="10169" checksum="8CF01A3032FF59A5" modified="2016-10-05" version="1" fragment="single">VGKLAHYGFDLVLVSIILAGIHRNTGLVFDTSHFSSVDFRKWFGQYLAFGERCFDRTVSLLRFSGYFKQKNITSESAHQKTCADIDNSMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBD9</accession>
    <name>A0A1A0HBD9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">LSM-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31779</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711713.1">
        <property type="nucleotide sequence ID" value="XM_018855915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBD9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBD9"/>
    <dbReference type="GeneID" id="30028891"/>
    <dbReference type="OrthoDB" id="429711at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033871">
        <property type="entry name" value="LSm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20971">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20971:SF0">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="98" mass="10407" checksum="11C35A7C67888346" modified="2016-10-05" version="1">MSEGLVKPSSDCGNGADTSNLRNDAGMLPLEVIDNAVGTQIKILLSNNKEFQGTLIGFDDFVNVVLQNVTEVDANGEKGRLIEKMLLNGAQIAMLCPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBP6</accession>
    <name>A0A1A0HBP6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chromosome segregation in meiosis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_23211</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in the control of DNA replication and the maintenance of replication fork stability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the fork protection complex (FPC) consisting of TOF1 and CSM3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the CSM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712069.1">
        <property type="nucleotide sequence ID" value="XM_018855258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBP6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBP6"/>
    <dbReference type="GeneID" id="30028234"/>
    <dbReference type="OrthoDB" id="437078at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031298">
        <property type="term" value="C:replication fork protection complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000076">
        <property type="term" value="P:DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012923">
        <property type="entry name" value="Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040038">
        <property type="entry name" value="TIPIN/Csm3/Swi3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220">
        <property type="entry name" value="TIMELESS INTERACTING-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13220:SF11">
        <property type="entry name" value="TIMELESS-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07962">
        <property type="entry name" value="Swi3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0236">DNA replication inhibitor</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation in meiosis protein 3" evidence="10">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="141"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="161" mass="18493" checksum="8769A1D005394658" modified="2016-10-05" version="1" fragment="single">SEKNDLGLDQPLKLGRVSRIAKLTDELLFETHRGLPQVRNNYPKLVRAMRKNDLAYASKVLKKQVSKSAAHQLKVSSEVENLQKVLLFYQLWCHGLFPKATFQDCLQMLRGHKSVAMREYRRGLLNNEIRRLKVEKGIIVEDPENEPGDSDDEFYDPASNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS5</accession>
    <name>A0A1A0HBS5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HotDog ACOT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711945.1">
        <property type="nucleotide sequence ID" value="XM_018858876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS5"/>
    <dbReference type="GeneID" id="30031852"/>
    <dbReference type="OrthoDB" id="331699at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03442">
        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000032">
        <property type="entry name" value="Acyl-CoA thioester hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="98"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="296"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="481" mass="53657" checksum="1C09B98F4A2740D3" modified="2016-10-05" version="1">MHTPLLAYQALETPAQHLGPEKPRGPIAQSHPAHLACATVNAEQRDNLVMAPVATWLDALTERSKQLKDGKTLDSYSYMNPKTTKVSERTRSESFSYVALPFKDDKWLCDSYINAFGRLRVGQMFQDLDALAGRIAYKHCAPAEPVNVTASVDRILMLRKVDEIQNYNFVLAGAVSWTGRSSMEITVKGYAFEDLVPTEFTEADLPSDKVFLTANFTFVARNPLTHKSFAINRLLPVSEQEWIDYRRAESYNAKKKLAAKNSSIIEPSDEETRLVHDMWKASKQLGAPSENLKLMKNSKFTSTLFMQPQYRNRHSYMIFGGYLLRQAFELAYCAAAAFTSAGPRFVSLDSTTFKAPVPVGSVLSMDASVSYTEHLLDSTSASKLKSPFNLPPTNKISKNPEAFLLEPGTLVQVKVDTHITNLDSSESKECGCFIYSFFVPGSSSKHPLASVVPQTYSEMMDYVEGRRRAHDTANYVDAISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS9</accession>
    <name>A0A1A0HBS9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein BFR2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_31874</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AATF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711842.1">
        <property type="nucleotide sequence ID" value="XM_018855936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS9"/>
    <dbReference type="GeneID" id="30028912"/>
    <dbReference type="OrthoDB" id="5783963at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025160">
        <property type="entry name" value="AATF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039223">
        <property type="entry name" value="AATF/Bfr2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565">
        <property type="entry name" value="AATF PROTEIN APOPTOSIS ANTAGONIZING TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15565:SF0">
        <property type="entry name" value="PROTEIN AATF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13339">
        <property type="entry name" value="AATF-Che1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AATF leucine zipper-containing" evidence="5">
        <location>
            <begin position="179"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="4">
        <location>
            <begin position="400"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="487"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="513" mass="55936" checksum="6A69B8A745CAF2EF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBX7</accession>
    <name>A0A1A0HBX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_11914</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711903.1">
        <property type="nucleotide sequence ID" value="XM_018854126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBX7"/>
    <dbReference type="GeneID" id="30027102"/>
    <dbReference type="OrthoDB" id="4095767at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="27426" checksum="1DCBC456184CB539" modified="2016-10-05" version="1">MRSCEIAAGCTPSRTAIPLEPGLTLQFLDLPNARLALPFRDAGEPDKNPVLRTQSAVVLFENVRLKLEFYHALRLVNPNTFQGERARVLIIECGQGALQQGAPGLPGALLVNCQTPEALLLVLRNIPTTNDPRIGACVLNTVIIENLSAFYWAKRSVTKALASKWYMDLNARARVLMETYQCNVLVTMWDKNYERGFNARPAQEPAPLRLVDLTYTPAEFFEMPAYVFASRAPGSLQYIGTQWRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC10</accession>
    <name>A0A1A0HC10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cation efflux protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711938.1">
        <property type="nucleotide sequence ID" value="XM_018857433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC10"/>
    <dbReference type="STRING" id="869754.A0A1A0HC10"/>
    <dbReference type="GeneID" id="30030409"/>
    <dbReference type="OrthoDB" id="5382797at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="608" mass="66994" checksum="F86CAA8DBAED8707" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS3</accession>
    <name>A0A1A0HCS3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 15">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 15">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_30897</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 15"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712395.1">
        <property type="nucleotide sequence ID" value="XM_018855734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCS3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCS3"/>
    <dbReference type="GeneID" id="30028710"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23283">
        <property type="entry name" value="beta-trefoil_MIR_PMT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF50">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="593"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="635"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="664"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="689"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="333"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="395"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="17">
        <location>
            <begin position="463"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="760"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="821"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="861"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="900"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="947" mass="106529" checksum="117DEA6B8581D55F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCZ8</accession>
    <name>A0A1A0HCZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">AAA-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_40694</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712382.1">
        <property type="nucleotide sequence ID" value="XM_018857301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCZ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HCZ8"/>
    <dbReference type="GeneID" id="30030277"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000110">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="233"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="555"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="152"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="825" mass="89668" checksum="A5EB62AEF8C4D0AB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDA3</accession>
    <name>A0A1A0HDA3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_139213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712373.1">
        <property type="nucleotide sequence ID" value="XM_018854452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDA3"/>
    <dbReference type="GeneID" id="30027428"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="158" mass="17392" checksum="018C4E25EDF23C99" modified="2016-10-05" version="1">MPPTSVSRRAPSGAEPRNRLAGGYRPPLVLNTPLPEKYGDLGVVMMLPRHILKDAGKIPQLAAQTILGEGRKKGSGRSCAAQMAHGWNRDGRRRTPVHLRPGLNLRNIGAARRNMLVLESCGQPHAINAFNFFFLSFFIDAIRCVFARCWPGSSLHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDG2</accession>
    <name>A0A1A0HDG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_39700</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712552.1">
        <property type="nucleotide sequence ID" value="XM_018857168.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HDG2"/>
    <dbReference type="GeneID" id="30030144"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18579">
        <property type="entry name" value="ABC_6TM_ABCC_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18603">
        <property type="entry name" value="ABC_6TM_MRP1_2_3_6_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000565">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000020">
        <property type="entry name" value="ABC metal ion transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000013">
        <property type="entry name" value="ABC transporter C family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000997">
        <property type="entry name" value="Multidrug resistance-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044746">
        <property type="entry name" value="ABCC_6TM_D1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056227">
        <property type="entry name" value="TMD0_ABC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF443">
        <property type="entry name" value="MULTIDRUG-RESISTANCE LIKE PROTEIN 1, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24357">
        <property type="entry name" value="TMD0_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="429"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="934"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="973"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1073"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1157"/>
            <end position="1178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1184"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="295"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="604"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="14">
        <location>
            <begin position="938"/>
            <end position="1217"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="1256"/>
            <end position="1488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="855"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="855"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="866"/>
            <end position="875"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1493" mass="167022" checksum="01C04E600F3045A3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE44</accession>
    <name>A0A1A0HE44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_40263</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712875.1">
        <property type="nucleotide sequence ID" value="XM_018857249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE44"/>
    <dbReference type="STRING" id="869754.A0A1A0HE44"/>
    <dbReference type="GeneID" id="30030225"/>
    <dbReference type="OrthoDB" id="47494at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178:SF1">
        <property type="entry name" value="FAD-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47178">
        <property type="entry name" value="MONOOXYGENASE, FAD-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="402" mass="44816" checksum="9617177B1E6FFAC0" modified="2016-10-05" version="1">MTLGNKQILISGAGIVGLLAAQALKARGVDFVIFDRDRDVHFREDAGWAITLHWALDTFLSLLPENLASEIYEAQVRKDFHLCDTGTFRYINASDGSTILSIPPSQRLRVRREQIRRILLKGIDVHWNCKLTRVAHLKCGRVVAQCESGREFQGDVLLGCDGANSQIRRLFCGDAGRLEVLPVRFCGAKVKISRAEEAHLARQFDPLLFQGTVPENETFFWFSMLSTPDYTLEEDGYFAQVNLSWKIRGQSDEPFGSATEKADAMLAHARGLHPQLHQLVARAAKSPENLVEIKLADWPEVRWDTHQGSVVLLGDVAHAMTMYRGEAANHGIADVADFIGQTDRYLKGDIPWADAVSNYCEQVYLRAQEAVLLSRQACLDAHDMSKITAGSASPLLTKRKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEA0</accession>
    <name>A0A1A0HEA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SPRY-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_77681</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712723.1">
        <property type="nucleotide sequence ID" value="XM_018858744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEA0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEA0"/>
    <dbReference type="GeneID" id="30031720"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12910">
        <property type="entry name" value="SPRY_SSH4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035780">
        <property type="entry name" value="SPRY_Ssh4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050618">
        <property type="entry name" value="Ubq-SigPath_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12864">
        <property type="entry name" value="RAN BINDING PROTEIN 9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="81"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="379" mass="43089" checksum="CB4FE99B19372EA9" modified="2016-10-05" version="1">MNLLLNTECMDWLPLREDRLVLQSSSHSITESSLLLFSVLRLKKIRSIQEDNRVYLNLNLDEQELYFQAKDFLTANPYLEGDLTLSQNLSIQEKGVRAWEFVRDSMLTNNDILIIQKYEINFFKRLECSVLTNLPIPKANEVYYFESKIFSLPNPEETILSIGISVKPYPWFRLPGRHAHSISYDSDGHTRHNEPFPISNEAPFPKLIEGDVVGIGYKVRSGTVFFTRNGKKLSELRINGHSKKFKPQANGQIFPIIGANNLCSIHVNIGQLGFVFIEGNVKKWGFAPLQGNGPAPPAYNPFNDDILLERSDVDELNGLTDRENDFPPDFLDIDILSGEHNENGLDTLGNDLITMHSLLPDLPPCYDQSSAHAEYYVQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HED3</accession>
    <name>A0A1A0HED3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712759.1">
        <property type="nucleotide sequence ID" value="XM_018853919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HED3"/>
    <dbReference type="GeneID" id="30026895"/>
    <dbReference type="OrthoDB" id="4076003at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="425" mass="47914" checksum="D3D40F76C682B4CD" modified="2016-10-05" version="1">MSIDSYKSSIIAMLINELPIQVNKRHLDIDIAEESRGLNNILLKRLQAKLDGAQKNSLVLKTKSLSCVNRPRRPGLRTHTSKLHLIDDTTLTSLPFPPPAPSKRFKPKFLSASHITQNSEFLRDESQLLYSVDLARATLDGTLFAESIDLSKQDTTMNGTICDYENDTLEETFDDSQDPIVLVEDYMTEAQSKETVFRKKSLANIKKRLREKSLRPSLTPNSASPGSFKVDFGRCNLPRVSTPGNENLEAIFGKIPGSEKLKYCTLCDKPLYEISSVLSSTEAMNTSHAANLSHAYQEFVCWGCIDTYEEVFNELYEAETKHSEEVARQSNHAIHQTSNSILDIDYMSLMTSEEQHEKTQNRGDSTQPQKKRKFSTDLISRLQRLSAVSQPKNGSAEWVAGWQEKLRRSLNNVLTNAFASDDQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEE4</accession>
    <name>A0A1A0HEE4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_39790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712768.1">
        <property type="nucleotide sequence ID" value="XM_018857176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEE4"/>
    <dbReference type="STRING" id="869754.A0A1A0HEE4"/>
    <dbReference type="GeneID" id="30030152"/>
    <dbReference type="OrthoDB" id="164921at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019431">
        <property type="entry name" value="DUF2417"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052370">
        <property type="entry name" value="Meta-cleavage_hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43139:SF52">
        <property type="entry name" value="SI:DKEY-122A22.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10329">
        <property type="entry name" value="DUF2417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="284"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="561" mass="63835" checksum="62F669C558282FDA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEM1</accession>
    <name>A0A1A0HEM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_160885</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713065.1">
        <property type="nucleotide sequence ID" value="XM_018854623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEM1"/>
    <dbReference type="GeneID" id="30027599"/>
    <dbReference type="OrthoDB" id="8964589at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009970">
        <property type="entry name" value="HC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07382">
        <property type="entry name" value="HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291811" evidence="2">
        <location>
            <begin position="29"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="166" mass="18785" checksum="29388DA397B3F53C" modified="2016-10-05" version="1">MAALPHRHGLSTLFFCTTLHHLSRLLFSLRTASISNPHPPHKAVFHKAVFHKAAFHKAVSHKLYPINQYPHKPVSHKPESHKAVFHKAVFHKPYPHKPVSHKPVSHKPVSHKPESHKPVSHKPVSHKPVFHKAVSHEPVSHKPVSHKPVSHKPVSHKPVFHKPVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEQ4</accession>
    <name>A0A1A0HEQ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_39944</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713100.1">
        <property type="nucleotide sequence ID" value="XM_018857209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEQ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HEQ4"/>
    <dbReference type="GeneID" id="30030185"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="292" mass="32533" checksum="5CD2DE1B48F413EC" modified="2016-10-05" version="1">MSGLQENTKIYTLNDGNKIPAVGLGTWRATSEDNIADVVASAIKNGYRHIDTAAIYGNEEDVGKGIKASGVPREEIFVTTKLWNSDHKNVRKAFEESLRKLQLDYVDLYLIHWPIDGSFGDKNAKISDSVETYLELQKLKQEGKAKSIGVSNFSKGKIESLLAHPEVTIKPAVNQIEAHPLLPQYELYDYLKKNDIYIEAYSPLGSADSPLLKEKNIVKIAEKNNVEPAQVLISWAIQRGTVVLPKSVKAARLLSNKKTFQLSDEDFEVLNQLAAVEGVQRLIDPLPVLFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEU3</accession>
    <name>A0A1A0HEU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HBS1-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_54351</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712924.1">
        <property type="nucleotide sequence ID" value="XM_018858291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEU3"/>
    <dbReference type="GeneID" id="30031267"/>
    <dbReference type="OrthoDB" id="4024467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015033">
        <property type="entry name" value="HBS1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08938">
        <property type="entry name" value="HBS1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HBS1-like protein N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="95" mass="10994" checksum="E08A4D7AE5BEBB48" modified="2016-10-05" version="1" fragment="single">MDDDYENYSGEDEFNEDALNNADYDALYAALPEAKAQLQSYNPQIQDLDIKEALYYNYFEIPAALEELKSKFPRKKEELPPPRLSKLAMLAKLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF10</accession>
    <name>A0A1A0HF10_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712978.1">
        <property type="nucleotide sequence ID" value="XM_018857175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF10"/>
    <dbReference type="GeneID" id="30030151"/>
    <dbReference type="OrthoDB" id="5299849at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018815">
        <property type="entry name" value="Incr_loss_mito_DNA_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28029:SF1">
        <property type="entry name" value="PROTEIN ILM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10311">
        <property type="entry name" value="Ilm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="167" mass="18800" checksum="0902EDD414241F92" modified="2016-10-05" version="1">MQFLTAKTVLYVRLAGLTCVCFFLLKKPQILVNANITVLLGRAMNLPIVQATPSNPLFGVLAVAIALLVVGDLVPVLADNVVYFETLVPTRLLFFFVLASFCLVSDYAVVANNLVFSYLFLEIWLNFLVFNNLRDEKYYRAKEYLETHGAELREMADAQVMPIGEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF41</accession>
    <name>A0A1A0HF41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_9113</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713230.1">
        <property type="nucleotide sequence ID" value="XM_018859318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF41"/>
    <dbReference type="STRING" id="869754.A0A1A0HF41"/>
    <dbReference type="GeneID" id="30032293"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF52">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="644"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="679"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="714"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="740"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="435"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="506"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA22749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="785" mass="90066" checksum="0E38FB67C6288E08" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF68</accession>
    <name>A0A1A0HF68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nucleolar</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_29364</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713260.1">
        <property type="nucleotide sequence ID" value="XM_018855389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF68"/>
    <dbReference type="STRING" id="869754.A0A1A0HF68"/>
    <dbReference type="GeneID" id="30028365"/>
    <dbReference type="OrthoDB" id="2019504at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010301">
        <property type="entry name" value="RRP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026">
        <property type="entry name" value="NNP-1 PROTEIN NOVEL NUCLEAR PROTEIN 1 NOP52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13026:SF0">
        <property type="entry name" value="RIBOSOMAL RNA PROCESSING 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05997">
        <property type="entry name" value="Nop52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="32930" checksum="31F9F4A1BFE35815" modified="2016-10-05" version="1">MAKETSAFVRKLAHNDPAIRKSAFAALTKYLQSPSGQKLRFHDLEKLWKGLYYSMWFCDKPIPQQNLAGDLGELFAKTVPQNKLADFHRAFWAVLMREWHSIDKWRLDKFLMLVRRAVRNSFFRLAEHQWPQLEVSQFLAVLEEYPLSNNVKFPQSLAYHVCDIYLDEIEYVIFKDFRDYSEENDDDEESESEDESGDDAELPAKSTPKAPKPSLGEKETEARKADIIAQTPVSLLLGPFEKLASGAKNKAMRSKVKEEILEDSRVLKWTGAEPASSDSEDEWTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF96</accession>
    <name>A0A1A0HF96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL11m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_40067</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713054.1">
        <property type="nucleotide sequence ID" value="XM_018857225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF96"/>
    <dbReference type="STRING" id="869754.A0A1A0HF96"/>
    <dbReference type="GeneID" id="30030201"/>
    <dbReference type="OrthoDB" id="1091498at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000005">
        <property type="entry name" value="Mitochondrial 54S ribosomal protein YmL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000004">
        <property type="entry name" value="Mitochondrial 54S ribosomal protein YmL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006519">
        <property type="entry name" value="Ribosomal_uL11_bac-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01632">
        <property type="entry name" value="L11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8 11" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="10">
        <location>
            <begin position="8"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="9">
        <location>
            <begin position="71"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="143" mass="15182" checksum="619CFA63616DD336" modified="2016-10-05" version="1">MSAKNVMVKLIVGAGQAAPAPPVGPALGSKGVKAIDFCKEFNARTAHYNPGTPIPVLLTIKPDRSFNFEMKSPPTSWLLLKAANAKKGSDKPTQNIVGTISLKHVYEIAKIKKTDERHKNVDLKAICGAVILTAKAVGVLVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFE6</accession>
    <name>A0A1A0HFE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Peptidase S59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_35048</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713350.1">
        <property type="nucleotide sequence ID" value="XM_018856561.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HFE6"/>
    <dbReference type="GeneID" id="30029537"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="11">
        <location>
            <begin position="476"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1401" mass="152716" checksum="CA15A75C9F8DDA1E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFE7</accession>
    <name>A0A1A0HFE7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="8">ATase</shortName>
            <ecNumber evidence="3 8">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_31493</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="3 8"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713118.1">
        <property type="nucleotide sequence ID" value="XM_018855864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFE7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFE7"/>
    <dbReference type="GeneID" id="30028840"/>
    <dbReference type="OrthoDB" id="191723at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11907">
        <property type="entry name" value="AMIDOPHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000485-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="537" mass="59510" checksum="1B7C0241AA71AB11" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFK0</accession>
    <name>A0A1A0HFK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_15328</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713153.1">
        <property type="nucleotide sequence ID" value="XM_018854584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFK0"/>
    <dbReference type="GeneID" id="30027560"/>
    <dbReference type="OrthoDB" id="1612078at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="2">
        <location>
            <begin position="121"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="678" mass="76365" checksum="218D4AECEFB9553C" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM2</accession>
    <name>A0A1A0HFM2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_9143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SF3A2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713266.1">
        <property type="nucleotide sequence ID" value="XM_018859325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFM2"/>
    <dbReference type="GeneID" id="30032300"/>
    <dbReference type="OrthoDB" id="10250970at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
        <property type="entry name" value="SF3A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031781">
        <property type="entry name" value="SF3A2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23205:SF0">
        <property type="entry name" value="SPLICING FACTOR 3A SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16835">
        <property type="entry name" value="SF3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="U1-type" evidence="10">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="250" mass="29141" checksum="5E03FB4DB4959CDF" modified="2016-10-05" version="1">MDYSDRVNSKKGAGALADKHESNVHTKRRIKELLTTQVLDLDRDPYVFRNHLGVLECRLCLTTHTNEASYISHLGGKKHGLNLERRRILDEKYNKAPGQNTGVSMTNVEKRHWKPIGRPSVKVTKIRLPKLLRMGMLVQAQFPQISVKEPFFRFMSYFEMSQKNQGASKDFLSKEKTDYEEDEDVDPSKWQYLVLSAEPYENVAISFPGAKQVDTPPGDEMSQNYWWHWDPDSREFFLQVLFKDEKLDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ2</accession>
    <name>A0A1A0HFQ2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_75984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713296.1">
        <property type="nucleotide sequence ID" value="XM_018858458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ2"/>
    <dbReference type="GeneID" id="30031434"/>
    <dbReference type="OrthoDB" id="4096984at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="402" mass="45656" checksum="5F3677FEE3479DD4" modified="2016-10-05" version="1">MSRYNGLSFFARAPLTYETLAERCRVFGRQNKHPPAQLVDYILANRKLQKINRGLGTGLRFGTLGSSLGLPLNVSSARLEQDLWLFVCKYWAPCVASDHVVTTYVCTNKNGNVSTLFRLVRENFASKLSGNVWPSTGPVTALTHVLLRENDFHGCFRLIDDTVNSAESMDLRRHQLLRQAAKTGVAAFTCGLGLSVWYTLLATSDILGLLDGVADLWPVLALSLASGLGTFLGFQWLKAASLPRILWRPHTSMWHRVLHFEEALFVNKIVTYYEEYNEVNVKNFHLSEVREQARLDVLDQSDYELHLPDPASGADDHDKIHATEALTRYFRQELYLRKMVWRPFKEQKMFLDFWLSHGDSYEWVEPDQDPAEMAFLRPLEDAAKPVLSLGSGVQARGSNTRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFQ5</accession>
    <name>A0A1A0HFQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Altered inheritance of mitochondria protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_75893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIM32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713169.1">
        <property type="nucleotide sequence ID" value="XM_018858440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFQ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFQ5"/>
    <dbReference type="GeneID" id="30031416"/>
    <dbReference type="OrthoDB" id="10253744at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03062">
        <property type="entry name" value="TRX_Fd_Sucrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009737">
        <property type="entry name" value="Aim32/Apd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902">
        <property type="entry name" value="ACTIN PATCHES DISTAL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31902:SF7">
        <property type="entry name" value="ALTERED INHERITANCE OF MITOCHONDRIA PROTEIN 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06999">
        <property type="entry name" value="Suc_Fer-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00040895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="290" mass="32699" checksum="BFF65DA2DFDAF146" modified="2016-10-05" version="1">MLSLSRQALCTYRTYATKFIQSCPKPTYDTGCTHCQIPEFPQDKQINFEHSLNGTSPIPWKHVLVLLHGVGDFADMASKINLIPGSMAQDFEILKRNHLSALHPVTLSNALISGEPQDATKRHRVRIYPDNVEVDFLPHHVQQFMEHYLLPEEAPQEVYNPFVKDQSTSKTRKLHPGLFKETSPQKDLVLICGHAKRDIRCGVLAPLLEQEFLKVLEREGIAGNVDVGLISHIGGHAYAGNVIFFPANPSRMPVTWYERVFPENVQGIVQATIKEGKIIKDLYRGQPSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFT5</accession>
    <name>A0A1A0HFT5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_75939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713238.1">
        <property type="nucleotide sequence ID" value="XM_018858452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFT5"/>
    <dbReference type="GeneID" id="30031428"/>
    <dbReference type="OrthoDB" id="496981at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="37719" checksum="EDA7F669A25978FF" modified="2016-10-05" version="1">MSLLVPSECDFEDAHSIPGLESAYERKLVAAREGHPEAYRWEFETVPGFFQQAAPETDDLLFRYTESNMGRTKPWPQIEAELAHLNETAPENVLYKLLICARHGQGYHNYIVDKYGLEAWDDKWYCMGTDGEVEYGPDPMLTDLGIAQAQENNRAWTREVRHHQAPIPSKFYVSPLQRSCWTCVYTWDGLRPADRKPVVVEKMRETLGRNLCDKRSLKTVIELRFGKHGFETEPGFAEEDPLFTPEREAADDLAMRINSVCQDLFEEDWDCVNGVVDKSKAAQNSVISTTTHAGTIRLFIVVLGHRRFTLSTGGMVPIVVKATRTGAERCAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFU3</accession>
    <name>A0A1A0HFU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ARM repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_34776</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713336.1">
        <property type="nucleotide sequence ID" value="XM_018856525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFU3"/>
    <dbReference type="GeneID" id="30029501"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF80">
        <property type="entry name" value="SUPPRESSOR PROTEIN MPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="3">
        <location>
            <begin position="99"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="121"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="164"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="200"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="237"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="393"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="504" mass="57393" checksum="D48DB6966DFE54BD" modified="2016-10-05" version="1" fragment="single">MSLTNLSLTSSRSKLTLGHSHNLSINSASSITEPTTPPSVGSTPKLYFWDSNKSNGNLILPSFNLESIPTYNNLHSRKISVESSGSDKENRPVFGYQQSRREGGKIDREYLISILKTPLSELSGEILRLAKDQHGCRFLQKRLDESYVPDVPSRKANYEIIFEQVKMSLYELIIDPFGNYLVQKLIDYVQTPDLDFCLEALNSNMFLISINQHGTRAFQKIIDKINTETQFNMLVRGLKPYIIELIKDLNGNHVIQKVLNKYRPEKCQFIYDSILADLLTVATHKHGCCVLQKCLNHVTAAQHMAFAEIILNYDNFSDLVNDQFGNYVLQYLISIDSIDVNFKLYQNIYEYGLTDLCVLKFSSNVIEKLMMNCFKNETKLIAFSNLKFTIIGHLLNGDLNKLINNAYGNYVVQTLTDILINTKAQYFIELPRGGQNILPSLRKLLDNDFQSSPDTLQVQIVKKWFQDCKIDSTFGKKIQSKINTILSGSVRTYHRKSHDASSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFW5</accession>
    <name>A0A1A0HFW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA methyltransferase 1, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_93982</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713356.1">
        <property type="nucleotide sequence ID" value="XM_018859378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFW5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFW5"/>
    <dbReference type="GeneID" id="30032353"/>
    <dbReference type="OrthoDB" id="270651at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18105">
        <property type="entry name" value="SpoU-like_MRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047182">
        <property type="entry name" value="MRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047261">
        <property type="entry name" value="MRM1_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004441">
        <property type="entry name" value="rRNA_MeTrfase_TrmH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013123">
        <property type="entry name" value="SpoU_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00186">
        <property type="entry name" value="rRNA_methyl_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46103:SF1">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08032">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00967">
        <property type="entry name" value="SpoU_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RNA 2-O ribose methyltransferase substrate binding" evidence="10">
        <location>
            <begin position="110"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="404" mass="44903" checksum="1CC9773039887F83" modified="2016-10-05" version="1">MRNYHTSSILKAIRPAFKSRDSRGGVRSFDKNFDASGAKKEIKIWDQKGISKHEFFIRKYGNISPEERKRLDEKVEKQRFLREQRKRHERGDSYDRPRRETISLNPLCEYIFGTHSVISALTAGKREAFSTLYIHNIKEHTEKILGLAKKFGVRVVEKKSKGELNTLSSNGVHNGVVLETKPLILPEIASLTNNFDGISGSYEVSLIDDATNGEIKRMNDIVRSAQSSSGNFPLGIYVDGVTDPQNLGNIVRSAYYLGADFMVVPESETARLGPVTAKAAAGALDLFTIYKTNSSLELIDSAKKNGWSIVSTSSREREDDLGDMKSNHRRHVAGKYIDQNELPRIMSAAPMLIVLGSEGAGIRTNIKLRSDFLVGWKKGRLGQSSIDSMNVAVAAGLIIAKILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGC3</accession>
    <name>A0A1A0HGC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WDR59/RTC1-like RING zinc finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_76273</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713695.1">
        <property type="nucleotide sequence ID" value="XM_018858502.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HGC3"/>
    <dbReference type="GeneID" id="30031478"/>
    <dbReference type="OrthoDB" id="311712at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035859">
        <property type="term" value="C:Seh1-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049567">
        <property type="entry name" value="WDR59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049566">
        <property type="entry name" value="WDR59_RTC1-like_RING_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46170:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17120">
        <property type="entry name" value="zf-RING_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <keyword evidence="3 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="105"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="193"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="WDR59/RTC1-like RING zinc finger" evidence="7">
        <location>
            <begin position="1304"/>
            <end position="1360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="483"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="708"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="804"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="809"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="825"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1081"/>
            <end position="1092"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1364" mass="153456" checksum="B3ADC44E8F7CE9C4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGG7</accession>
    <name>A0A1A0HGG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-prolyl cis-trans isomerase D</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rotamase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_9397</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713566.1">
        <property type="nucleotide sequence ID" value="XM_018859377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGG7"/>
    <dbReference type="STRING" id="869754.A0A1A0HGG7"/>
    <dbReference type="GeneID" id="30032352"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000022">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000029">
        <property type="entry name" value="peptidyl-prolyl cis-trans isomerase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4 9" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="9">
        <location>
            <begin position="302"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00076602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="365" mass="39568" checksum="CA9C2C09BF93677C" modified="2016-10-05" version="1">MSKVFFDLTANGIPKGRVVFKLYNDVVPKTTENFRALCTGEKGNSAESGKPLHYKGSTFHRVIKSFMCQGGDFTHGTGIGGESIYGNKFEDENFKLTHDKPFLLSMANAGPNTNGSQFFITTVPTPHLNGKHVVFGEVIQGKSVVRELERCEKGDGDKPTEDWVIADCGELPADYVPEASAVSDDGTGDIYEAVLADDDKIDQSQPQSVLDAAKFLKDLGTKLLKEGKLEKALEKYTKGSGFLNDFTPESLSEELTNEKTSLSVSLALNAALVALKLKDGKKAVRAADEALAFSQADEKTTAKAWYRKGSGYLLLKDEDNAEAAFKSALALSPSDAAVLKGLRDVKELAQVRTAKQKKAMSKFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS3</accession>
    <name>A0A1A0HGS3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_97221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713534.1">
        <property type="nucleotide sequence ID" value="XM_018859447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS3"/>
    <dbReference type="GeneID" id="30032422"/>
    <dbReference type="OrthoDB" id="4079944at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="523" mass="59872" checksum="00C3EF9F31AE10A6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGU4</accession>
    <name>A0A1A0HGU4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">FAD/NAD(P)-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_29636</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713583.1">
        <property type="nucleotide sequence ID" value="XM_018855451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGU4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGU4"/>
    <dbReference type="GeneID" id="30028427"/>
    <dbReference type="OrthoDB" id="202203at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="5">
        <location>
            <begin position="3"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="377" mass="39538" checksum="9F8BD0A0B5234A09" modified="2016-10-05" version="1">MPKIVIIGGSFAGLNALATIFKTAGSTSIDATLVAPNSHAYFNVASPRLLSEPDKFSETTVPVSEAVQKHSRGKATFLRGLATAVDFDKREVSVQLMAGGHKTLPYDILVVASGTEAKWAGYKVNTNHEDAHKAIVAANKALKNALSVAVVGGGPTGTEVAGEIAHVFKKAKVTLYTGGLGPLAGAAAKLSGKALKKLGALGVELVNGVQVQSVSGTAGGKSTVALANGQSREHDLVLESYIVRPYSEFLPSAVKDGKGYVVTDDHLLVKGQRSVVALGDIVSGSSKSLVDLKMRQAGLFRATIQNLLRDVQNPEFLPGAKTAKEGLEYQPVTHTMMVPISRDGGVGMVFGISLPNFAVHYGKAKTFMLEKARSEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGV1</accession>
    <name>A0A1A0HGV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytochrome b5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_105077</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome b5 family. MAPR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713885.1">
        <property type="nucleotide sequence ID" value="XM_018853785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGV1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGV1"/>
    <dbReference type="GeneID" id="30026761"/>
    <dbReference type="OrthoDB" id="10257697at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050577">
        <property type="entry name" value="MAPR/NEUFC/NENF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281:SF76">
        <property type="entry name" value="CALCUTTA CUP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10281">
        <property type="entry name" value="MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="3">
        <location>
            <begin position="62"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="20990" checksum="2911520A20838D38" modified="2016-10-05" version="1">MNPSESRTRFNVLDIVRVFAGILLINALASWWFTSSPTWGYTGKYIDQRYLKHRLFGRQVTLTPQELRLRDGTDTSLPIYIGIGGRVYDVTASRHIYGPKGPYAFFSGRDAARAFVTGCFQKEDEFTHDIRGLDPEEARADIQSWQDYFERSSKYWYVGTVIHEPLTGDPPAPCDHQKYPHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH55</accession>
    <name>A0A1A0HH55_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713812.1">
        <property type="nucleotide sequence ID" value="XM_018855490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH55"/>
    <dbReference type="GeneID" id="30028466"/>
    <dbReference type="OrthoDB" id="19690at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18834" checksum="34169C4467A1E02F" modified="2016-10-05" version="1">MLLVFRLRPMYIACHSVIPSRTLFGWFTKYNKRLIYPPSPNQITTKNRLFPTYIERPTELGTVLLGKEPLLLNFTFPGDEKCNQVTLTLFDVLSDSKKYPLDESKSVALANIACDSPGGRELQQTYAVSKIPTIVLLKKQMEADRFIPTSSKRVGDELVGFIKGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH57</accession>
    <name>A0A1A0HH57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="8">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_76364</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713811.1">
        <property type="nucleotide sequence ID" value="XM_018858517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH57"/>
    <dbReference type="STRING" id="869754.A0A1A0HH57"/>
    <dbReference type="GeneID" id="30031493"/>
    <dbReference type="OrthoDB" id="17199at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160203">
        <property type="term" value="P:mitochondrial disulfide relay system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000003">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF0">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 8" id="KW-0274">FAD</keyword>
    <keyword evidence="3 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="10">
        <location>
            <begin position="73"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="178" mass="20495" checksum="0AA6351C1CDEE509" modified="2016-10-05" version="1">MTQEITESKDETPKPVFGASGRQIVYDKDGKPCRTCNTLLDFQMVSGKSKVPSKTEEKIKKTENPASTYKQDDPPDVEKLGRSSWTLLHLIAATYPEHPTTKEQADMKQFVKLFGNFYPCWFCADDFKKYMVSNEPETETQDSLGKWLCGAHNEVNKKLGKPQFDCTLWKKRWKDGWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH91</accession>
    <name>A0A1A0HH91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_107537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714030.1">
        <property type="nucleotide sequence ID" value="XM_018853846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH91"/>
    <dbReference type="GeneID" id="30026822"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="89" mass="9956" checksum="AA6B01FCFE4765F9" modified="2016-10-05" version="1">MAVSCRHSVFYSLERVKIIIRLSFVLGFSYGCPSKRLAVLREMRRGLFPVRGSNRMSYACIPSCGVAKGATPDLWCPFRLVGWASPRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHE1</accession>
    <name>A0A1A0HHE1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein L37ae</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_85515</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713739.1">
        <property type="nucleotide sequence ID" value="XM_018859250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHE1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHE1"/>
    <dbReference type="GeneID" id="30032225"/>
    <dbReference type="OrthoDB" id="10258345at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000002">
        <property type="entry name" value="60S ribosomal protein L37a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="100" mass="10918" checksum="5597B36E99FF2F6E" modified="2016-10-05" version="1">MSKSSKRLATKRTKKVGITGKFGVRYGSSLRRQTKKLEVQQHAKYDCSFCGKRTVKRGAAGIWTCKACKKTVAGGAYTVSTAAAATVRSTIRRLRDMAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHG5</accession>
    <name>A0A1A0HHG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_36141</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BUB2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713926.1">
        <property type="nucleotide sequence ID" value="XM_018856693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHG5"/>
    <dbReference type="STRING" id="869754.A0A1A0HHG5"/>
    <dbReference type="GeneID" id="30029669"/>
    <dbReference type="OrthoDB" id="10263206at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902543">
        <property type="term" value="P:negative regulation of protein localization to mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031030">
        <property type="term" value="P:negative regulation of septation initiation signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000035">
        <property type="entry name" value="Cell cycle arrest protein BUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000026">
        <property type="entry name" value="Mitotic check point protein (Bub2)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF263">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="6">
        <location>
            <begin position="44"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="315" mass="35968" checksum="AD8EB44AC1FF8D6C" modified="2016-10-05" version="1">MDVKGRKKDSIEQFIELPQILVDNGLSQLRYMILIEGLSIPDGYEQCPYRSYVWSILCKVPVYPAYKYQQTISKISRNLSPEVYQKIKNDTFRTLMNDKAFHSRVSEDSLMRILAAIATSIPENKVGYVQGLNVLLAPIAYTCYKSEPQAFAILHHLITKQIPLYITPNLDGVHTALSLVDLVLKIIDPVLSEFLDSKFLKAEIYAFPSVLTLCACTPPLKSLIKMWDFLFAYGTHMNLLFVVAQLIRNRSTLLKSLQPMKELRNFPALDENNIIKLSLSFIPELPKDLYDMITRHGFDPSVPKELAIFIKAHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHI2</accession>
    <name>A0A1A0HHI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Exocyst complex component Sec3 PIP2-binding N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76369</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713818.1">
        <property type="nucleotide sequence ID" value="XM_018858518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHI2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHI2"/>
    <dbReference type="GeneID" id="30031494"/>
    <dbReference type="OrthoDB" id="27109at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028258">
        <property type="entry name" value="Sec3-PIP2_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048628">
        <property type="entry name" value="Sec3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20654">
        <property type="entry name" value="Sec3_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01313">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="6">
        <location>
            <begin position="87"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="192"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="511"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="825"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="833"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1153" mass="130045" checksum="7A89D85BD8DAC1BF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHT7</accession>
    <name>A0A1A0HHT7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PLP-dependent transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_76433</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713889.1">
        <property type="nucleotide sequence ID" value="XM_018858527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHT7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHT7"/>
    <dbReference type="GeneID" id="30031503"/>
    <dbReference type="OrthoDB" id="691673at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008793">
        <property type="term" value="F:aromatic-amino-acid transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009074">
        <property type="term" value="P:aromatic amino acid family catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050859">
        <property type="entry name" value="Class-I_PLP-dep_aminotransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42790:SF2">
        <property type="entry name" value="AROMATIC AMINO ACID AMINOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="6">
        <location>
            <begin position="289"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="478" mass="53382" checksum="A4B4B93F047C9323" modified="2016-10-05" version="1">MSHLISKRASSRKISHFANDTLQEAPKGFEPHPSLLHLDYGTPNDGFFPVKSIGISVKNTAFEVVDAIFDGSKSVGDVTSLQESKVLVSNRASDPRIIDIATGLQYSEIKGIPQLQKFTREVVVKAHPPIYSEWKTIISGGAGEGLNKAIDVFLDEGEVVLLEEFTFTPILQNIQNAGAIPIPIKLDLNTSSDQANGIDLEYLTNLLENWDDAKPELKGIKPNALYTISSGQNPTGLHSKLGKHDFAIIEDDPYGYLTLPPYEESNVAKRPICLEVEDYFQNHLIPSYLTIDTSGRVLRIETFSKLFAPGLRLGYMVGHEKVIDAIARYSAVVTRSSSGVSQLLVNNVIEQNFGGVDGWFKWILKLRGAYAKRRNVLLYNLFELKAYKKGYLDLIDPRAGMFVCVVINFPVNTDIKKKLALLQWKFRIKMAVDPEFSLERGNFFRLTFAPVETETEIAEGACRFSAAVYDFFEKGLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHX6</accession>
    <name>A0A1A0HHX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol-phosphate mannosyltransferase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_36565</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit to the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714245.1">
        <property type="nucleotide sequence ID" value="XM_018856759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHX6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHX6"/>
    <dbReference type="GeneID" id="30029735"/>
    <dbReference type="OrthoDB" id="2014333at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013174">
        <property type="entry name" value="DPM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433:SF0">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08285">
        <property type="entry name" value="DPM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="93" mass="10318" checksum="6602DAE5E66DD1C6" modified="2016-10-05" version="1">MTKATETFVALFAVGAVYFALYAELIPSGKLVHDEILPYLPFWAVVTFGAYALGTLGWGVLTFKNKEGSYKELMQQIDEAKAFYKEKGVDLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHY1</accession>
    <name>A0A1A0HHY1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PLP-dependent transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_34283</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714250.1">
        <property type="nucleotide sequence ID" value="XM_018856460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHY1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHY1"/>
    <dbReference type="GeneID" id="30029436"/>
    <dbReference type="OrthoDB" id="6752799at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF55">
        <property type="entry name" value="GLUTAMATE OXALOACETATE TRANSAMINASE 1, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="32"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="402" mass="44139" checksum="3FC380BBF98BE3E5" modified="2016-10-05" version="1">MAPHTFFALPDASEDPIVALMNAYAADQHPAKIDVSIGVYKSEEGDPNYVFPSVKAAKSILAADDPGHCYTQMSGIPAFVAQAQKTIFGDSHADVASVQSVAGSGALHLAFALLVELGLTDFHVGEPCWGNYQGMVEHAGGLYIAYSYYDAATHTVDFDAIVHALSSAREGAVFVLQAVCHNPTGLDLTQAQWLRVFGLIRARKLFALFDIAYQGFASGDVDQDAWAIREAWRQGLEFMVCQSYSKNMGLYSERVGCTHVRARDEASAQRLRSALTRLVRTEFSFAPAFGARVAAILQENPQVRPIWLQDVRDVTARLRDVRQKMLETFEAMGTPGTWDHVTRATGMFWFTGLSRVQTQKLQEEHHIYGTSNGRVNVAGLNNSNIAQYCAAVDAVVRKYPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH0</accession>
    <name>A0A1A0HIH0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Actin-related protein 2/3 complex subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34287</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the Arp2/3 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ARPC3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714277.1">
        <property type="nucleotide sequence ID" value="XM_018856462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH0"/>
    <dbReference type="GeneID" id="30029438"/>
    <dbReference type="OrthoDB" id="200404at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005885">
        <property type="term" value="C:Arp2/3 protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034314">
        <property type="term" value="P:Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051654">
        <property type="term" value="P:establishment of mitochondrion localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1760.10:FF:000002">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.10">
        <property type="entry name" value="Actin-related protein 2/3 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007204">
        <property type="entry name" value="ARPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036753">
        <property type="entry name" value="ARPC3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12391">
        <property type="entry name" value="ARP2/3 COMPLEX 21 KD SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04062">
        <property type="entry name" value="P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016315">
        <property type="entry name" value="ARP2/3_P21-Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69060">
        <property type="entry name" value="Arp2/3 complex 21 kDa subunit ARPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="19921" checksum="FB9C8B6533C70A0F" modified="2016-10-05" version="1">MPAYHSTFLGEASERVVGNIVLLPIHTQYRGPLYPPSSEYDIIEETLDLFRANSFFKNFDIKGPADRLLIYGILFVSDCLSKLNARVSYKEAVRALNNLALDSFALPGDIGFPMNEVYQAPATKNDAELLRSYLQQFRQELADRLLKRIYADSDAVPSKFWLAFTRKRFMNRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIS1</accession>
    <name>A0A1A0HIS1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714382.1">
        <property type="nucleotide sequence ID" value="XM_018856685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIS1"/>
    <dbReference type="GeneID" id="30029661"/>
    <dbReference type="OrthoDB" id="4093324at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="451" mass="51236" checksum="C4EB332DE51B5102" modified="2016-10-05" version="1">MEKTKSSLIFQHVVDSAPLGILPELVETYLLSLMRSDNMNAVVSLVHILLHSNFKHYRFSNQFWSLLTSKSASLGHHGAASLAYHEIINPFVNFSGDGPISEENEHILFLLLPTTIESLAIVFAQNGNHVAVEGLRQYFKRFYSNFGHRDVYQTLYITKVEFLAAAGLFSEALNAFTALAIKYKGHIGYRDPKDLVYSLKYASHMHYKKRKMNIAQNISHIVQEDSDKFNNSFCPDIKFNDYTFERSLYWAILDGVLSVQDLPKFKSLLSKKLKELMARHDSVVDRLLAFIFNNHSSMGKFVIACLCETGHIPEAIAVINKLPSLFPRVKETACITEIDFQRIFKALEIQFQAQESHDCKALQSQLMTSYELCQRLVRNSSMSRACFRSFLQCYFSSPLANIDEINQISESWKSRGGKPIGLGSSAYKNALSCGLSSLDLRNYITENPIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIY2</accession>
    <name>A0A1A0HIY2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">rRNA methyltransferase 2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_30321</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714442.1">
        <property type="nucleotide sequence ID" value="XM_018855617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIY2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIY2"/>
    <dbReference type="GeneID" id="30028593"/>
    <dbReference type="OrthoDB" id="20105at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF18">
        <property type="entry name" value="RRNA METHYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="8">
        <location>
            <begin position="18"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="23347" checksum="57656E39AAC8D74F" modified="2016-10-05" version="1">MDRQSADPYTKKSLADGYRSRAAYKLIEINQKHKIFKKSTKNIVDLGFAPGAWTQVALAEFAKLGLSPKILGVDLINCAPPPGSHFIEGDIFSRKTHQEIDSFFSEEGIDVVLSDMMCNSTGNGELDHYGSMDICEGVLILTNRLLRQKGTLVMKYFSGSQDREMRQKLEKMFDRVFTMKPHACRQSLREMYMIGLGKRIQKSNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ16</accession>
    <name>A0A1A0HJ16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nramp-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714358.1">
        <property type="nucleotide sequence ID" value="XM_018855588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ16"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ16"/>
    <dbReference type="GeneID" id="30028564"/>
    <dbReference type="OrthoDB" id="409173at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015086">
        <property type="term" value="F:cadmium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001046">
        <property type="entry name" value="NRAMP_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01197">
        <property type="entry name" value="nramp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037982">
        <property type="entry name" value="Nramp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706:SF50">
        <property type="entry name" value="MANGANESE TRANSPORTER SMF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11706">
        <property type="entry name" value="SOLUTE CARRIER PROTEIN FAMILY 11 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01566">
        <property type="entry name" value="Nramp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00447">
        <property type="entry name" value="NATRESASSCMP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="416"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="568"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="304"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="600" mass="65180" checksum="513FE1634697E157" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ46</accession>
    <name>A0A1A0HJ46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_30240</name>
        <name evidence="2" type="ORF">METBIDRAFT_33894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000017">
        <property type="protein sequence ID" value="OBA16119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709231.1">
        <property type="nucleotide sequence ID" value="XM_018856417.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714344.1">
        <property type="nucleotide sequence ID" value="XM_018855581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ46"/>
    <dbReference type="GeneID" id="30028557"/>
    <dbReference type="GeneID" id="30029393"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA16119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8581" checksum="187380EAB0C20B9D" modified="2016-10-05" version="1" fragment="single">MSDILVSNISDDGWKVQGRKRRNSRKRPERSVTMATAPGLILTDQKGSLGSYPSMKQPSSNLNPFLVLAEISEEDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJB3</accession>
    <name>A0A1A0HJB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="3 7">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_30450</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="3 7"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714586.1">
        <property type="nucleotide sequence ID" value="XM_018855638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJB3"/>
    <dbReference type="GeneID" id="30028614"/>
    <dbReference type="OrthoDB" id="60315at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000042">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015892">
        <property type="entry name" value="N-myristl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00976">
        <property type="entry name" value="NMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="9">
        <location>
            <begin position="46"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="10">
        <location>
            <begin position="221"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="432" mass="47793" checksum="40D762A2064ABEE3" modified="2016-10-05" version="1">MADKQPRLIEELLQLLAPQQKTMADHKFWNTQPVPALGEEVQSEGPIDRAKTAQDVPATPLPLLAGFEWATVDIDVPAQLDEVYRLLHDHYVEDQDATFRFKYSQAFFRWALQPPGWRRDWLVGVRVQGRGTLVAFVAAVPVTLKLAGPGATVPAVEINFLCVHKKLRSKRLAPVLIREITRRVNRHGVWHALYTAGVVLPSPVATCRYTHRPLDWAKLHDVGFLPLPPGQTKASMAARYTLAPAPRTPGFRPMEAADLDQVFDLLHAYQQRFQMVQVFGKAELAHWLLGGECGGAVVKAYVVERAGRICDFVSYYLLPFSVLDHAVHTELGVAYLFYYATDSHTAAPPVYQARLTGLVTDALITARAFGVDVFNALTCQDNTLFIDAAKFGPGDGLLHYYLFNYRAKPIDGGLDGRQQVGSAGSGVGVVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJJ4</accession>
    <name>A0A1A0HJJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="4 12">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">5-proFAR isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_34285</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="4 12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714489.1">
        <property type="nucleotide sequence ID" value="XM_018856461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJJ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJJ4"/>
    <dbReference type="GeneID" id="30029437"/>
    <dbReference type="OrthoDB" id="446074at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011858">
        <property type="entry name" value="His6/HISN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02129">
        <property type="entry name" value="hisA_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="8 12 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="273" mass="29526" checksum="527822521AA22024" modified="2016-10-05" version="1">MTRFRGCIDIHAGQVKQIVGGSLTADDTHHSADTKENFVSTKPSSHYARLYQARGVEGCHVIKLGSNPANDEAARLACATWPGHLQVGGGITDDNALTWLDTYHASHVIVTSWLFSKTAAGQTVLDWQKLARLSERVGKTRLIVDLSCREVAGAQQKKWVVSINKWQTLTDSELGPDFLAAVADYCDEFLIHAADVEGLCKGIDEDLVASLGRWCPPGFAGKIVYAGGAKLVSDLALVDRLSAGKVDLTFGSALDIFGGDLVKFEDVVRWGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJL8</accession>
    <name>A0A1A0HJL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714563.1">
        <property type="nucleotide sequence ID" value="XM_018855634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJL8"/>
    <dbReference type="GeneID" id="30028610"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="82" mass="9125" checksum="51E221BFE95C06DE" modified="2016-10-05" version="1">MGAYLIVYTPLLVATCTCYSSIFLYSKRNVAISLEKAPCTLGSLGVSYAQYEDLLPQCNDLPANPKTNISVLVDLMRQRRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJM2</accession>
    <name>A0A1A0HJM2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sec7-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_76961</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714568.1">
        <property type="nucleotide sequence ID" value="XM_018858613.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HJM2"/>
    <dbReference type="GeneID" id="30031589"/>
    <dbReference type="OrthoDB" id="10258608at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056604">
        <property type="entry name" value="GBF1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23325">
        <property type="entry name" value="TPR_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="533"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1424" mass="160095" checksum="9595F07E8112ADC1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJM9</accession>
    <name>A0A1A0HJM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">GTP-binding protein RHO3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_36544</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714855.1">
        <property type="nucleotide sequence ID" value="XM_018856755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJM9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJM9"/>
    <dbReference type="GeneID" id="30029731"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030950">
        <property type="term" value="P:establishment or maintenance of actin cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090338">
        <property type="term" value="P:positive regulation of formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010590">
        <property type="term" value="P:regulation of septum digestion after cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000780">
        <property type="entry name" value="Rho GTPase Rho3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="222" mass="24592" checksum="08C70DF8E3037565" modified="2016-10-05" version="1">MPLCAGGNKTIQRKIVILGDGACGKTSLLNVFTRGYFPQVYEPTVFENYVHDIFIDGQAVQLSLWDTAGQEEFDRLRALSYSDTHCIMLCFSIDSPDSLENVQLKWVGEIADHCVGVKLVLVALKCDLRNQEEDPLSVHEDESFNPYSNQSSGMPGSHRRLVPYEDGLSVAKKVGALRYLECSAKKNRGVNEAFSEAARCALNAKPKGLNDNEPEKKSCVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJQ5</accession>
    <name>A0A1A0HJQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_36734</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714731.1">
        <property type="nucleotide sequence ID" value="XM_018856772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJQ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJQ5"/>
    <dbReference type="GeneID" id="30029748"/>
    <dbReference type="OrthoDB" id="6612291at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF16">
        <property type="entry name" value="HIGH GLUCOSE SENSOR RGT2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="39"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="536"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="672" mass="73697" checksum="00998308B27ACCCC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJT8</accession>
    <name>A0A1A0HJT8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA repair protein RAD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_34398</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714638.1">
        <property type="nucleotide sequence ID" value="XM_018856469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJT8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJT8"/>
    <dbReference type="GeneID" id="30029445"/>
    <dbReference type="OrthoDB" id="2801544at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18008">
        <property type="entry name" value="DEXDc_SHPRH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014905">
        <property type="entry name" value="HIRAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08797">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00910">
        <property type="entry name" value="HIRAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="443"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="803"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA24157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="872" mass="100705" checksum="B1A75F239BC47DF6" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJU9</accession>
    <name>A0A1A0HJU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_30607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714776.1">
        <property type="nucleotide sequence ID" value="XM_018855667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJU9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJU9"/>
    <dbReference type="GeneID" id="30028643"/>
    <dbReference type="OrthoDB" id="2420608at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018465">
        <property type="entry name" value="Scm3/HJURP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10384">
        <property type="entry name" value="Scm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="416"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="555" mass="63139" checksum="E470B954624E486D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK33</accession>
    <name>A0A1A0HK33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Heat shock protein 9/12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714842.1">
        <property type="nucleotide sequence ID" value="XM_018855678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK33"/>
    <dbReference type="STRING" id="869754.A0A1A0HK33"/>
    <dbReference type="GeneID" id="30028654"/>
    <dbReference type="OrthoDB" id="2348401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007250">
        <property type="entry name" value="HSP9_HSP12"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04119">
        <property type="entry name" value="HSP9_HSP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002590">
        <property type="entry name" value="HSP9/HSP12_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0346">Stress response</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="65" mass="6890" checksum="412EA1EA3937FD7E" modified="2016-10-05" version="1">MSDTGRKSVGDKISSAVKPDSQKGTGELLKDNVTDKVDNTVGDNTRSGDKSFLQQASDAVFGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK58</accession>
    <name>A0A1A0HK58_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase eIF4A</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Eukaryotic initiation factor 4A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_10425</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family. eIF4A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714753.1">
        <property type="nucleotide sequence ID" value="XM_018853767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK58"/>
    <dbReference type="STRING" id="869754.A0A1A0HK58"/>
    <dbReference type="GeneID" id="30026743"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000089">
        <property type="entry name" value="Eukaryotic initiation factor 4A-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000031">
        <property type="entry name" value="Eukaryotic initiation factor 4A-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 19" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="18">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="53"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="234"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="14">
        <location>
            <begin position="22"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA24272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="396" mass="44386" checksum="8A9F9FD275EA3EF6" modified="2016-10-05" version="1">MSNGITEVDANLIESNYDNVVYNFDDLNLKPEIVRGIFGYGYEAPSAIQQRAILPITEKRDVLAQAQSGTGKTATFTISALQNIDENEKATQALILAPTRELALQIQNVISQIGMYLNVSVHASIGGTSMADDIEAFRSGVQIVVGTPGRVYDMIDRRYFKTHKIRMFILDEADEMLSSGFKEQIYNIFKLLPETTQVVLLSATMPQDVLEVTTKFMNNPVRILVKKDELTLEGIKQYYINVEEEAYKFDCLCDLYDSISVTQAVIFCNTRSKVETLTAQLKDNNFTVSSIHADLPQSDRDTIMQEFRSGSSRILISTDLLARGIDVQQVSLVINYDLPANKENYIHRIGRGGRFGRKGVAINFVTSQDVGMMREIEKFYSTQIDEMPADIGALFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKY0</accession>
    <name>A0A1A0HKY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SCA7-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30786</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715033.1">
        <property type="nucleotide sequence ID" value="XM_018855709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKY0"/>
    <dbReference type="STRING" id="869754.A0A1A0HKY0"/>
    <dbReference type="GeneID" id="30028685"/>
    <dbReference type="OrthoDB" id="21678at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904802">
        <property type="term" value="P:RITS complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037804">
        <property type="entry name" value="SGF73"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47805:SF1">
        <property type="entry name" value="SAGA-ASSOCIATED FACTOR 73"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="3">
        <location>
            <begin position="217"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="110"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="449"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="146"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="482" mass="52315" checksum="86A872FF868E869E" modified="2016-10-05" version="1">MSRRSDQGSQSRTVTLGQVRALRASHSESSSLAKKQISWKDFGVYLDKKLDRTLDPSFVGSTATSSALVNILSRSSLLSQVIKFQICTSCNRPIGEAILATHHQKCVEMKHKRPESEAPSGSAVSKRKRGRSVNPLLDIEYSSVDSSRGASPTPATPSSSQNTQGRPKKKYKKSQMQKEKEAANAAAAAAAAAEAANNRASTAAHAKKKKQAKVKSATKAKGPVDVEKQCGVPLPLGGFCARSLTCKTHSMGAKRAVPGRSAPYDVLLQQYQKRNQAKLAASNALAAQRRENAQLTGMSVGDSENGDGLGNLHIVLDSDTETQLVLEGLSKNCPAPLERKIMLPVRHRHKFLYMREAYANALITNGSASSDSLAHSADVTLANQAISGSIGGLQGRCALINVDNKSSNGDTQTYASIPGEIYQVRAPLKAILMTAQYNHQQQQLFQQQIMKLQQQQLLAKQRQQQLQRQQQQQQQQQHQNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H227</accession>
    <name>A0A1A0H227_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47846</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709372.1">
        <property type="nucleotide sequence ID" value="XM_018858230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H227"/>
    <dbReference type="STRING" id="869754.A0A1A0H227"/>
    <dbReference type="GeneID" id="30031206"/>
    <dbReference type="OrthoDB" id="1704689at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005761">
        <property type="term" value="C:mitochondrial ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.370.10:FF:000001">
        <property type="entry name" value="COX11 cytochrome c oxidase assembly homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00155">
        <property type="entry name" value="CtaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003465">
        <property type="entry name" value="PRK05089.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320:SF3">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21320">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="Ctag/Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA17977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="257" mass="29305" checksum="B50EF04593E67C92" modified="2016-10-05" version="1">MLGLLRLIKNNLATVKHDLRYKSTKKFGTYRPLRQDSSAKKAAQDSLQRMHWEQLERMKFRNRSAAYYSGSVIMLFAGLSCVAVPFYRYLCKRTGWGGNPITDTRLFTPDKVIPVETNKRIRVSFTCQASGILPWKFTPLQKEVYIVPGETALVFYRAKNLSKEDIVGMATYSVSPDHVAPYFNKIQCFCFEEQRLAAGEEVDMPVFFFIDPDFAKDSAMRNIDDVVLNYSFFKASHQDGSLSPVPAGYMELKAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H275</accession>
    <name>A0A1A0H275_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709422.1">
        <property type="nucleotide sequence ID" value="XM_018854455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H275"/>
    <dbReference type="GeneID" id="30027431"/>
    <dbReference type="OrthoDB" id="4084707at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="337" mass="37807" checksum="19E7EB57D0F9B89A" modified="2016-10-05" version="1">MVVRAAISTAVNLVPFSDRLPQRVHNLMGSGASRLADLELSPDEVRPLATPSQKIRKKMREKRPATERSSLALGRNSSLDAEDDGNDTARDTDTQSLRTERVELYLCEQHDEFTSLINRNLKEVVRQQQQRQAEEAGWRRLVLEIMMARAGDDATQKLVLLVARAQDSIYASVPFIHSERRLQTSAGEYGSSALKVDSAELLDQLDYAQKVALLRHLMVDLGMRADADDIAEFTRGSLLDDDTSIADKLELLVLLLVRLWFIGLRCLVPVLQHIYTRFKENDMLLVNNRNFSRLLTVLARAMVTVEERLNTFSTGLSRLNSQASSRPSSQTLATSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4L7</accession>
    <name>A0A1A0H4L7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="10">DOHH</shortName>
            <ecNumber evidence="10">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LIA1</name>
        <name evidence="12" type="ORF">METBIDRAFT_13778</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="10">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709554.1">
        <property type="nucleotide sequence ID" value="XM_018854426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4L7"/>
    <dbReference type="STRING" id="869754.A0A1A0H4L7"/>
    <dbReference type="GeneID" id="30027402"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000099">
        <property type="entry name" value="Deoxyhypusine hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="HEAT" evidence="11">
        <location>
            <begin position="242"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="317" mass="35509" checksum="AE2D1CEB574ED649" modified="2016-10-05" version="1">MSEIDVDSATIDQLRDVLCNKSGDVALANRFRALFNLKCLGADSEDEETVHKAIDYIAECFADSYELLKHEVAYVLGQIHNVYANKHLKNVLADTNQQVMVRHEAAEAMGAIGDSECLEMLEDYFKNDPSIEIRQTCELAIERINWEKSAAAKTETLEKSAFTSIDPAPPMATDKESKVDKLQKILNDQEVPLFERYRAMFRLRDLATDEACLALATGLDDPSALFKHEIAYVFGQLCNPVTVPSLIKILKDDREAGMVRHEAAEALGSIATDECLPVLKSFLNDEEEVVRDSAIVALDMYEYENSNELEYATVKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4R2</accession>
    <name>A0A1A0H4R2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">mRNA export factor GLE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Nucleoporin GLE1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33647</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709561.1">
        <property type="nucleotide sequence ID" value="XM_018856363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4R2"/>
    <dbReference type="STRING" id="869754.A0A1A0H4R2"/>
    <dbReference type="GeneID" id="30029339"/>
    <dbReference type="OrthoDB" id="420884at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006409">
        <property type="term" value="P:tRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.510">
        <property type="entry name" value="GLE1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012476">
        <property type="entry name" value="GLE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038506">
        <property type="entry name" value="GLE1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960">
        <property type="entry name" value="GLE-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12960:SF0">
        <property type="entry name" value="MRNA EXPORT FACTOR GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07817">
        <property type="entry name" value="GLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="137"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00026227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="501" mass="57746" checksum="C13B2A13B1B9A3A2" modified="2016-10-05" version="1">MRFGIPADHDVAALPRPHLSVPEKTTSPSPSSSFSDMTSTQSASQILNESLIQLDTLHIRNLSSRIDEKFQKWEIFINESKIAVNSKLSYIDNEFNQKIQALQLDVQKIIDEQIRLREEEKKRLMNLEIEKQRKIKEEADRKRREEEEEERKRRDHQKKLAEEKRLADEKRLAEEHKLKKEKEENEKLERKKEELLKARAEKEAAAKKASAFANPADIEKALLKYRKDIEDIKTNIVGEVDKDKELKKYIGAIKRKVNTKLGQLSNSLRQLDLVSKEITSLIQPLASQPLAYKWILNFVLKAIVSQAETEVTVKPAAALPLGRLAYNLLCNLNGLEFYLSARFVKKCCLVIGYTGTIDTEEGRVRMGWRRSDGKWESEVKYEERMGGILSVWAVIGWVNQTTSLLLFNMKAEWMFLARLLNTKPELLGDVHFVAVCNWWEAAGEKFFKTFGKQAQKLLTVSITDWVPLGVSRSFPAATRLQILGEDLFTKNNFNTLKEMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4W3</accession>
    <name>A0A1A0H4W3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_47549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709611.1">
        <property type="nucleotide sequence ID" value="XM_018858188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4W3"/>
    <dbReference type="STRING" id="869754.A0A1A0H4W3"/>
    <dbReference type="GeneID" id="30031164"/>
    <dbReference type="OrthoDB" id="5584247at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052246">
        <property type="entry name" value="Cell_Polariz_PKAAnc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155:SF1">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13155">
        <property type="entry name" value="A-KINASE ANCHOR PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="435"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="461" mass="53402" checksum="CA3842B7491A8FDE" modified="2016-10-05" version="1">MTEKYQLDNPNAHYLANLSNLNLTNNNIQLGRLPTLGEILSNKTKSPISLYNFYLYMKNVENNADYLDFWFDLINHLNLCKHYVQGLRESLVRQSVHSNNFGSNNPFNSSPNHNRDSFPLSEKSKYKSLSLSVLLELILNDQILEDNDSNRLSQFLRGEINLDTVDPKLKELIEAYNAEESIAQPSPTMDRNSFYTSSDRQFSSQSRLLDEPVESPQISPIQQGDFRPPDVLSQNDRIRKPRRHSSLNPSLLENLIKDSPVSNLFISRQNLRESSHNLLLKYFVEDSEKSLEISPHLNDKIVKAIQIDGRDDPSVFDGVKLYVFDRLENEHLPNFLNFIAIKNINRSVNARVIFGFFMSFISFWIAFSLIFLDEKKRYRCVIIFTFFCGFYGLMTSIYRIDPLLAFSARSESYIPSHTFIRVEDTFIHKLLMKRATWVLFLVLLLTAIFSVPFCLVPGKHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Y0</accession>
    <name>A0A1A0H4Y0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Helicase SWR1</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Helicase swr1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_61237</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SWR1 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709540.1">
        <property type="nucleotide sequence ID" value="XM_018858315.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H4Y0"/>
    <dbReference type="GeneID" id="30031291"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140849">
        <property type="term" value="F:ATP-dependent H2AZ histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18003">
        <property type="entry name" value="DEXQc_SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000655">
        <property type="entry name" value="Protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.850:FF:000012">
        <property type="entry name" value="protein PHOTOPERIOD-INDEPENDENT EARLY FLOWERING 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.850">
        <property type="entry name" value="SWI2/SNF2 ATPases, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014012">
        <property type="entry name" value="HSA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF1">
        <property type="entry name" value="HELICASE SRCAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07529">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51204">
        <property type="entry name" value="HSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSA" evidence="22">
        <location>
            <begin position="406"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="776"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="21">
        <location>
            <begin position="1314"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="182"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="530"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="663"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1510"/>
            <end position="1552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="21"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="541"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="577"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="665"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1511"/>
            <end position="1523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA19005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1576" mass="179906" checksum="3FDC1ECE9409843A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H584</accession>
    <name>A0A1A0H584_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CUE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_13504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709728.1">
        <property type="nucleotide sequence ID" value="XM_018854377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H584"/>
    <dbReference type="STRING" id="869754.A0A1A0H584"/>
    <dbReference type="GeneID" id="30027353"/>
    <dbReference type="OrthoDB" id="4080456at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CUE" evidence="3">
        <location>
            <begin position="85"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="37640" checksum="6477D5B7B34AEF31" modified="2016-10-05" version="1">MSCPERRRRANNPPVFSAPPLFSTPPSMADLPELEFLVDAFPHYPRLELASRLLCAASPEVLFNELAVENSSTSKNSQPSEAARGFPPEVTALAELFPSAHKDSLAAALQQNGGCMDKTIEALLEHQPVHRLAAVSGLPEPELRPYVARHKHDVLRALADVIAQYRRRTRVRTSRVQARAGTALADRDTYVLNERSAAFVLLLESVWAEPALRGINFDFLRKLLVFFQGDVSRVLDVARLYIEAGSQAVTAYHSTANFSTVDVSTVYLSTVGFFHAVPLLQAWNSFLFMCPGNWLVALEHAENSPGPPHHASQLSCPLAFKTVAAKASTGPSVRKRWNYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5C9</accession>
    <name>A0A1A0H5C9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nuclear cap-binding protein complex subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">80 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_33442</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NCBP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709773.1">
        <property type="nucleotide sequence ID" value="XM_018856297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5C9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5C9"/>
    <dbReference type="GeneID" id="30029273"/>
    <dbReference type="OrthoDB" id="10252707at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000056">
        <property type="entry name" value="Sto1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIF4G-like type 1" evidence="11">
        <location>
            <begin position="380"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="MIF4G-like type 2" evidence="12">
        <location>
            <begin position="659"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF09088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="958" mass="110179" checksum="35DE1AAEC1FC8603" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5G8</accession>
    <name>A0A1A0H5G8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sec63-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_46915</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709866.1">
        <property type="nucleotide sequence ID" value="XM_018858101.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H5G8"/>
    <dbReference type="GeneID" id="30031077"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18020">
        <property type="entry name" value="DEXHc_ASCC3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000198">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000231">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000241">
        <property type="entry name" value="Antiviral helicase SLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF13">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="276"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="500"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="1132"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="1361"/>
            <end position="1531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1923" mass="217611" checksum="9717BD73BDE3672B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5H2</accession>
    <name>A0A1A0H5H2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Negative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709700.1">
        <property type="nucleotide sequence ID" value="XM_018858098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5H2"/>
    <dbReference type="GeneID" id="30031074"/>
    <dbReference type="OrthoDB" id="2101473at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007396">
        <property type="entry name" value="TR_PAI2-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35802">
        <property type="entry name" value="PROTEASE SYNTHASE AND SPORULATION PROTEIN PAI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35802:SF1">
        <property type="entry name" value="PROTEASE SYNTHASE AND SPORULATION PROTEIN PAI 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04299">
        <property type="entry name" value="FMN_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010372">
        <property type="entry name" value="PaiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="212" mass="24684" checksum="A25CC08D43C39812" modified="2016-10-05" version="1">MYIPQHYKEESWDKKRKLIQDYPLATVITIDADGTPVANHFPFYLHEDEETGKKFLQTHLAKKNHQLPSLQDNNKVLVIFQSANSYISPSYYPEKERTHKFVPTWDFASLHIKGKLRVIDDFDFVRSQLNHFTDQQEAGRECPWKVDDAPENYTRLMQKAITGLEIEVVESDCKFKFEQKMTRENVDGTINGLACDGLHQISNFVKECNLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H671</accession>
    <name>A0A1A0H671_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arginase/deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33232</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the arginase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710112.1">
        <property type="nucleotide sequence ID" value="XM_018856247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H671"/>
    <dbReference type="STRING" id="869754.A0A1A0H671"/>
    <dbReference type="GeneID" id="30029223"/>
    <dbReference type="OrthoDB" id="288726at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11592">
        <property type="entry name" value="Agmatinase_PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.10:FF:000014">
        <property type="entry name" value="Arginase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291872" evidence="5">
        <location>
            <begin position="19"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="400"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="443" mass="49623" checksum="DF84EC36971A6FAC" modified="2016-10-05" version="1">MRSVDLLLLALAICGVNAQYKVSEKHVINTENEAPQLQDIWGEAWPFQGINTFAHLPYKQCLVDRELTYDIALIGVPFDTATSYRLGARFGPRAIRAASQRQTSFRGYNPRADFNPYTSWAKILDCGDIPVTPMDNYLAFKQMTMAFEELILEHSASAEDSKAPRYVALGGDHSVILPHLRSLHKIYGPINVIHFDAHLDTWKPDKYPSFWLTPQSEINHGSMLWKAFEEGLISTNNLHAGIRTKLSGPADLQDDDAQHWTRISADDIWIDGVQSVIDRILATVPENSPTYISVDIDVLDPGFGSGTGTQEPGGWLPRELLYVLRGIDLLSVVGADIVEVNPDFDHAEITSTNGAQVAYELITSMVKKGPLAFEQKANLERTAVHPVDGLSLLAEDHGLSFELKEKAEKLQQRLEDVEKLKVELEREIAQVKKLRMTVQRQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6W4</accession>
    <name>A0A1A0H6W4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710296.1">
        <property type="nucleotide sequence ID" value="XM_018856140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6W4"/>
    <dbReference type="GeneID" id="30029116"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="66" mass="7773" checksum="71DA502BE5A69A44" modified="2016-10-05" version="1">MSVLSFSLPWATTWLPSYVLRPERRIQYSQFRLLSRSVIKHYSICAISIAFRGSYLYSLTDQLLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Y2</accession>
    <name>A0A1A0H6Y2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA topoisomerase I</fullName>
            <ecNumber evidence="7">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">DNA topoisomerase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_13408</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6 7">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the type IB topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710195.1">
        <property type="nucleotide sequence ID" value="XM_018854356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Y2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Y2"/>
    <dbReference type="GeneID" id="30027332"/>
    <dbReference type="OrthoDB" id="47179at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000019">
        <property type="term" value="P:regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009303">
        <property type="term" value="P:rRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00659">
        <property type="entry name" value="Topo_IB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03488">
        <property type="entry name" value="Topoisomer_IB_N_htopoI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.41:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.10:FF:000003">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.11.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.15.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.11.10">
        <property type="entry name" value="DNA Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.15.10">
        <property type="entry name" value="Topoisomerase I, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.41">
        <property type="entry name" value="Yeast DNA topoisomerase - domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013034">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013030">
        <property type="entry name" value="DNA_topo_DNA_db_N_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001631">
        <property type="entry name" value="TopoI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025834">
        <property type="entry name" value="TopoI_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014711">
        <property type="entry name" value="TopoI_cat_a-hlx-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014727">
        <property type="entry name" value="TopoI_cat_a/b-sub_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013500">
        <property type="entry name" value="TopoI_cat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008336">
        <property type="entry name" value="TopoI_DNA-bd_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036202">
        <property type="entry name" value="TopoI_DNA-bd_euk_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013499">
        <property type="entry name" value="TopoI_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018521">
        <property type="entry name" value="TopoIB_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048045">
        <property type="entry name" value="Topoisomer_I_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051062">
        <property type="entry name" value="Topoisomerase_IB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290:SF3">
        <property type="entry name" value="DNA TOPOISOMERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10290">
        <property type="entry name" value="DNA TOPOISOMERASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14370">
        <property type="entry name" value="Topo_C_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01028">
        <property type="entry name" value="Topoisom_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02919">
        <property type="entry name" value="Topoisom_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00416">
        <property type="entry name" value="EUTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00435">
        <property type="entry name" value="TOPEUc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56741">
        <property type="entry name" value="Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00176">
        <property type="entry name" value="TOPO_IB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52038">
        <property type="entry name" value="TOPO_IB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="DNA topoisomerase I eukaryotic-type" evidence="9">
        <location>
            <begin position="320"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="126"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="6">
        <location>
            <position position="753"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="795" mass="91900" checksum="E120F17E0B3D7E4A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H708</accession>
    <name>A0A1A0H708_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SCF complex F-box protein MET30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33315</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710211.1">
        <property type="nucleotide sequence ID" value="XM_018856268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H708"/>
    <dbReference type="STRING" id="869754.A0A1A0H708"/>
    <dbReference type="GeneID" id="30029244"/>
    <dbReference type="OrthoDB" id="5580488at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043224">
        <property type="term" value="C:nuclear SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140299">
        <property type="term" value="F:molecular sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046685">
        <property type="term" value="P:response to arsenic-containing substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22147">
        <property type="entry name" value="F-box_SpPof1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000016">
        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit sconB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000715">
        <property type="entry name" value="F-box protein MET30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051075">
        <property type="entry name" value="SCF_subunit_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872">
        <property type="entry name" value="UBIQUITIN LIGASE SPECIFICITY FACTOR/HREP PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19872:SF9">
        <property type="entry name" value="UBIQUITIN-BINDING SDF UBIQUITIN LIGASE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="178"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="299"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="339"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="379"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="418"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="544"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="567"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="638" mass="72630" checksum="DF30B9902821F95C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H729</accession>
    <name>A0A1A0H729_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CNH-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_22587</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710237.1">
        <property type="nucleotide sequence ID" value="XM_018855176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H729"/>
    <dbReference type="STRING" id="869754.A0A1A0H729"/>
    <dbReference type="GeneID" id="30028152"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041675">
        <property type="entry name" value="PH_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057283">
        <property type="entry name" value="RGF3_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF1">
        <property type="entry name" value="RHO1 GUANINE NUCLEOTIDE EXCHANGE FACTOR TUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15405">
        <property type="entry name" value="PH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23582">
        <property type="entry name" value="WHD_RGF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="5">
        <location>
            <begin position="303"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="535"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="726"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="902"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1053"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1053" mass="120309" checksum="8318260A8DEC89F8" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H785</accession>
    <name>A0A1A0H785_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_32966</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MNN1/MNT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710479.1">
        <property type="nucleotide sequence ID" value="XM_018856176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H785"/>
    <dbReference type="STRING" id="869754.A0A1A0H785"/>
    <dbReference type="GeneID" id="30029152"/>
    <dbReference type="OrthoDB" id="430354at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000033">
        <property type="term" value="F:alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046354">
        <property type="term" value="P:mannan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022751">
        <property type="entry name" value="Alpha_mannosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31392:SF1">
        <property type="entry name" value="ALPHA-1,3-MANNOSYLTRANSFERASE MNN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11051">
        <property type="entry name" value="Mannosyl_trans3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="765"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="765"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="784"/>
            <end position="820"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="836" mass="95849" checksum="649EC8BBA40161C3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H794</accession>
    <name>A0A1A0H794_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Receptor/non-receptor type protein-tyrosine phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_45339</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710421.1">
        <property type="nucleotide sequence ID" value="XM_018857908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H794"/>
    <dbReference type="STRING" id="869754.A0A1A0H794"/>
    <dbReference type="GeneID" id="30030884"/>
    <dbReference type="OrthoDB" id="10253954at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001403">
        <property type="term" value="P:invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18533">
        <property type="entry name" value="PTP_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="50"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="236"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="306" mass="34963" checksum="F0658E4FFFB710A3" modified="2016-10-05" version="1">MIVKPRFLNISELEQRAGFRKISAYCDSHLSEALEPSSRSQWNVNAAFDPVNRNRNRYSNVLPWDKTRVSLSPTLGGSDYINASYVQLAGSKYIAAQGPLRNTVHHFWAMCFAQAQESGSLSIFIAMVTPLVELNREKCFQYWPTREEGAWDMGKMLDSDRLAPNDLRLSWVSEKVTDLFVLTRFRLQSGDVSKTVYHYYYLGWRDTLTPNSMQPLLLLSAEISALKQTDPNLVPVVHCSAGVGRTGTFIAIDHFIRADLAKDPETDIVFETVKTLRDHRMMMVQTVQQYLFLYQAAKRIHSIKQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7G7</accession>
    <name>A0A1A0H7G7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNase III domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710362.1">
        <property type="nucleotide sequence ID" value="XM_018857869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7G7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7G7"/>
    <dbReference type="GeneID" id="30030845"/>
    <dbReference type="OrthoDB" id="4087411at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040540">
        <property type="entry name" value="RNase_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18497">
        <property type="entry name" value="RNase_3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase III" evidence="2">
        <location>
            <begin position="148"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="410"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="422"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="508"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="543" mass="61495" checksum="EE1AACAF1AE157FE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7P8</accession>
    <name>A0A1A0H7P8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_45511</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710442.1">
        <property type="nucleotide sequence ID" value="XM_018857925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7P8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7P8"/>
    <dbReference type="GeneID" id="30030901"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="6"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="8">
        <location>
            <begin position="187"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="5">
        <location>
            <begin position="187"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="476"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="480"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="535" mass="59731" checksum="2492FF85855941DA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7S9</accession>
    <name>A0A1A0H7S9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_45237</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL43 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710679.1">
        <property type="nucleotide sequence ID" value="XM_018857897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7S9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7S9"/>
    <dbReference type="GeneID" id="30030873"/>
    <dbReference type="OrthoDB" id="88at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039927">
        <property type="entry name" value="Ribosomal_mL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007741">
        <property type="entry name" value="Ribosomal_mL43/mS25/NADH_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21396:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05047">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00916">
        <property type="entry name" value="L51_S25_CI-B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein/NADH dehydrogenase" evidence="7">
        <location>
            <begin position="32"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="142" mass="16183" checksum="DAB83EEA5DF5CF80" modified="2016-10-05" version="1">MPVKAIQKLSLARNGIGAFVKPCHKITVQFCNWGGSSQGVRDLLVDPEKRFQKFTTSNKDTMFEIVKKSGHPMLTFHYSTGKNSVVDVKNLVPTDIVAKLDEYIKRGGNNLFKFNHKVMSENESVRGVWSPFHEAKEHRHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7T6</accession>
    <name>A0A1A0H7T6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF431-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_212014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710570.1">
        <property type="nucleotide sequence ID" value="XM_018855050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7T6"/>
    <dbReference type="GeneID" id="30028026"/>
    <dbReference type="OrthoDB" id="373498at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18090">
        <property type="entry name" value="Arginine_MT_Sfm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007364">
        <property type="entry name" value="SFM1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35517:SF1">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE SFM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04252">
        <property type="entry name" value="SFM1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="23673" checksum="DC761CB91B459143" modified="2016-10-05" version="1">MKYVIEHMEEGFSEWVILEYSQIIRDVGKENLILTSLPKETTEKDIPDRLLSLGLQWTTDECVTLDGGLKSDRVCLLDPAATEDLLPEDASRFDYFVFGGILGSHPRIDRTGILRKKYGFAGRRLGALQMSTDTAIRTTQLIIEKQQKFEDIKFIDYPEIRYNKYESTEMPFRYVLDSEGIPILPEGMLKLIEKDADQSIDDLLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7V9</accession>
    <name>A0A1A0H7V9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_44598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710595.1">
        <property type="nucleotide sequence ID" value="XM_018857814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7V9"/>
    <dbReference type="GeneID" id="30030790"/>
    <dbReference type="OrthoDB" id="2536440at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42115">
        <property type="entry name" value="BETA-SYNTHASE (BETA-THIONASE), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42115:SF1">
        <property type="entry name" value="BETA-SYNTHASE (BETA-THIONASE), PUTATIVE (AFU_ORTHOLOGUE AFUA_3G08420)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="16"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="2">
        <location>
            <begin position="126"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="176" mass="19930" checksum="3896DE6F695BE104" modified="2016-10-05" version="1">MNPLLFHITDYRGATVEDLDIRPAISVNPSTPLKRAFEVAFENEFTFLPVIHEKNKRLLGVVKLLDPKPLEKTSSSTEPLTIEFMHWFSQKAKERYAKEDDKSNSKTPLNSKILRPKAAGGKHYQVLTPLTPLEELGKFFNSGNYFAIVTNGEGNLVYGVVTPEDLGKYEHSRPRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Z0</accession>
    <name>A0A1A0H7Z0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710538.1">
        <property type="nucleotide sequence ID" value="XM_018856187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Z0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Z0"/>
    <dbReference type="GeneID" id="30029163"/>
    <dbReference type="OrthoDB" id="8883818at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="672" mass="75092" checksum="884870713674CD77" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Z4</accession>
    <name>A0A1A0H7Z4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sure-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33064</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710630.1">
        <property type="nucleotide sequence ID" value="XM_018856209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Z4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Z4"/>
    <dbReference type="GeneID" id="30029185"/>
    <dbReference type="OrthoDB" id="4018688at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008252">
        <property type="term" value="F:nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291877" evidence="4">
        <location>
            <begin position="19"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="5">
        <location>
            <begin position="33"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="334" mass="36187" checksum="7926823FB372DA8C" modified="2016-10-05" version="1">MRLSSIFTTLATALSVSSAPVSNESECSSNKTILLTNDDGWASTNIRAAYRELKSAGHEVILVAPVQQRSGYGGQFGLPTSKNLTAGGDFNYPPAGAPSWGHEDNDMNIWYFNGTPSSCVAFGLNYIIPEYFGNKTIDLVVGGPNEGTNLSPGLYTLSGTVGAIYSAVNRGIPGIAFSGSMTNNSFFQDDISYQNDTNFGANILAKKVVDLVDTLFESFQNRPNMIPVTTGINVNFPAVGEDCTDPEYVYTRMTGIDSEIPDLTFNETSGMPVWKYGLYEATTKCVFGDCDLPSESWLLAEEKCKSSLTLFSIDYDASYKQSQAVKSFLAPIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H811</accession>
    <name>A0A1A0H811_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_213743</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710686.1">
        <property type="nucleotide sequence ID" value="XM_018855064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H811"/>
    <dbReference type="GeneID" id="30028040"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="198" mass="22175" checksum="DFCDDC94F4015504" modified="2016-10-05" version="1">MGRDLQPHRTCSAGAAWPISRHLGRHHSGSLSRHHSGSISRHLSKTLVEILVKVLVDFFVRILVDIIVEVLVDIIVEVSVDILVEVLVDTSVEVLVDILKNLSRHLSKSISRLLCKNLSRHHSGSISRHLSKTLVEILVKVLVVDFFVRILVDILVKVLVDFFVRILVDIIVEVLVDIIVEVLVDIIVEVLADILVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8B1</accession>
    <name>A0A1A0H8B1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Prefoldin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_43908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710871.1">
        <property type="nucleotide sequence ID" value="XM_018857715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8B1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8B1"/>
    <dbReference type="GeneID" id="30030691"/>
    <dbReference type="OrthoDB" id="2015447at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13525" checksum="61BFC9B9D2C620EA" modified="2016-10-05" version="1">MSLDPQALQKLLIEMDNQLNQSRAELSMCTVQLDRVDTNLRIVDTTCSRLAKLTSPGDSVWRGVGRAFVENDVSDYVGRIQKDKADFLETQKLLNTKKHYLETTLENTVKHMTDIVGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8B2</accession>
    <name>A0A1A0H8B2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cse1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_44913</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPO2/CSE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710652.1">
        <property type="nucleotide sequence ID" value="XM_018857851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8B2"/>
    <dbReference type="STRING" id="869754.A0A1A0H8B2"/>
    <dbReference type="GeneID" id="30030827"/>
    <dbReference type="OrthoDB" id="3268246at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061015">
        <property type="term" value="P:snRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005043">
        <property type="entry name" value="XPO2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013713">
        <property type="entry name" value="XPO2_central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF8">
        <property type="entry name" value="EXPORTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03378">
        <property type="entry name" value="CAS_CSE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08506">
        <property type="entry name" value="Cse1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="8">
        <location>
            <begin position="25"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="986" mass="110906" checksum="182DC6A7C68B441D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8E1</accession>
    <name>A0A1A0H8E1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710683.1">
        <property type="nucleotide sequence ID" value="XM_018854314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8E1"/>
    <dbReference type="GeneID" id="30027290"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="19661" checksum="807980BF576396DF" modified="2016-10-05" version="1">MDSMGPVVIASARGTPHEQHCAVGQTEPPWSGNSARTIPPVSMLIIGPPDFPATISTPGWRRWKSGCTSVGSPSSRGTHRNGIVGADDPAGTEIHSLWKRYAVILETSAGLGGMASLLWIWRGRAYGPWVMSRSPFQGMPACGLEGQIKLVGGILVCLTKEFVFPQARKSGSVWWKTWDYLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8I9</accession>
    <name>A0A1A0H8I9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_43791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710720.1">
        <property type="nucleotide sequence ID" value="XM_018857699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8I9"/>
    <dbReference type="STRING" id="869754.A0A1A0H8I9"/>
    <dbReference type="GeneID" id="30030675"/>
    <dbReference type="OrthoDB" id="199771at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11608">
        <property type="entry name" value="eIF2D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21156">
        <property type="entry name" value="PUA_eIF2d-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039757">
        <property type="entry name" value="EIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039759">
        <property type="entry name" value="eIF2D_SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048248">
        <property type="entry name" value="PUA_eIF2d-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058886">
        <property type="entry name" value="SWIB_eIF2D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057429">
        <property type="entry name" value="WH_eIF2D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12217:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26292">
        <property type="entry name" value="PUA_elF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26291">
        <property type="entry name" value="SWIB_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25304">
        <property type="entry name" value="WHD_eIF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="1">
        <location>
            <begin position="470"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="561" mass="62773" checksum="8C3DCCE14399291F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8M1</accession>
    <name>A0A1A0H8M1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ornithine carbamoyltransferase</fullName>
            <ecNumber evidence="3">2.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Ornithine transcarbamylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_78837</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710986.1">
        <property type="nucleotide sequence ID" value="XM_018858954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8M1"/>
    <dbReference type="STRING" id="869754.A0A1A0H8M1"/>
    <dbReference type="GeneID" id="30031930"/>
    <dbReference type="OrthoDB" id="10252326at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903269">
        <property type="term" value="C:ornithine carbamoyltransferase inhibitor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000017">
        <property type="entry name" value="Ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000009">
        <property type="entry name" value="Ornithine carbamoyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001986">
        <property type="entry name" value="PRK00779.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF3">
        <property type="entry name" value="ORNITHINE TRANSCARBAMYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="10">
        <location>
            <begin position="23"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="9">
        <location>
            <begin position="175"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="342" mass="37690" checksum="633E733B772079EF" modified="2016-10-05" version="1">MFKRYFSSSMAMKSTTPINTTPRHLVSISQLSAEEFSALVTKAQHFKNVVRSGDSAEIKKNHDKLLGKLVALLFSKRSTRTRISSEGAAAHFGAQPMFLGKDDIQLGVNESMYDTTKVISLMTSCVFARVNKHLEIQELCKHSSVPIINSLCDMYHPLQAIADVLSIKEAFGKTQGLKLAWIGDANNVINDLAIAALKVGISVSVLVPASVTFEPSIVGEAEKIVSESSGAVSFEVVNEPLKALKDANVIVTDTWISMGQESEKAEKLKQFAGYQITQQMLHDGQVNENWKFMHCLPRHAEEVADDVFYCDNSIVFEEAENRLYAAMAVIEAFVVNKGDLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8M7</accession>
    <name>A0A1A0H8M7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710760.1">
        <property type="nucleotide sequence ID" value="XM_018854203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8M7"/>
    <dbReference type="GeneID" id="30027179"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="16767" checksum="DF74BA2F97B97C57" modified="2016-10-05" version="1">MEWKISVKQQMHKIFGFKTGPHGVKMYAIPKLIHHCKDSIDLLTLKINLREAGDEVEGDVGPTTGRWIRGLKKALRFGAVILHGLTFVTALDVIFHDCLHVGKIVKGAEPLQPAAYTRVTSRGSTVSLKKEIGEDAVRNAQAPFEIQQSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8W9</accession>
    <name>A0A1A0H8W9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleoporin Nup159/Nup146 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32341</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710850.1">
        <property type="nucleotide sequence ID" value="XM_018856032.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H8W9"/>
    <dbReference type="GeneID" id="30029008"/>
    <dbReference type="OrthoDB" id="248320at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039462">
        <property type="entry name" value="Nup159/Nup146_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16755">
        <property type="entry name" value="Beta-prop_NUP159_NUP214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117289">
        <property type="entry name" value="Nucleoporin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup159/Nup146 N-terminal" evidence="5">
        <location>
            <begin position="216"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="822"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1010"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="678"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="826"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="904"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="917"/>
            <end position="928"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1321" mass="139035" checksum="DA88786919250CA0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H936</accession>
    <name>A0A1A0H936_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_43785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711043.1">
        <property type="nucleotide sequence ID" value="XM_018857698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H936"/>
    <dbReference type="STRING" id="869754.A0A1A0H936"/>
    <dbReference type="GeneID" id="30030674"/>
    <dbReference type="OrthoDB" id="10258858at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="72" mass="8522" checksum="D5090C7D63F652DC" modified="2016-10-05" version="1">MIIPIRCFSCGKVVGDKWETYLTLLQDETLTEGDALDQLKLKRYCCRRMVLTHVDLIEKFLRYNPLEKKEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H997</accession>
    <name>A0A1A0H997_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_199039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710970.1">
        <property type="nucleotide sequence ID" value="XM_018854907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H997"/>
    <dbReference type="GeneID" id="30027883"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="178" mass="20363" checksum="CA0004602F811358" modified="2016-10-05" version="1">MARAGVHIYSRDQDSVEICGVSAGRMDKGKVTLTIRELSIWTGRIRRGLPKACGKLPVEVLQYTSSDGMIRHRCRTNRWKNLVKAIGARQGLPQRPKTKQRNRRRCKGVMKEKENKGDGKKRDLVAGKQLRRKANEAGKKASGQTNGTKARRVRPKRDETRQTRTDEYTQKRSQQHQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA11</accession>
    <name>A0A1A0HA11_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_68681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711350.1">
        <property type="nucleotide sequence ID" value="XM_018858372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA11"/>
    <dbReference type="STRING" id="869754.A0A1A0HA11"/>
    <dbReference type="GeneID" id="30031348"/>
    <dbReference type="OrthoDB" id="2152029at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010424">
        <property type="entry name" value="EutQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019436">
        <property type="entry name" value="Say1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169:SF1">
        <property type="entry name" value="ACETATE KINASE EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36169">
        <property type="entry name" value="ETHANOLAMINE UTILIZATION PROTEIN EUTQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10340">
        <property type="entry name" value="Say1_Mug180"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="412" mass="47381" checksum="C67E522E3844A712" modified="2016-10-05" version="1">MSLRSICVLLKLPLDLLIVVLRFYIFGGLRFRKYSRQLINCLRLRVYRAALTVDIPDGKLIGPHTNSFLIRKVIPFILSTLVKNCSAYGERFDAQSFWLVKQKNRKPSDPVIIFCHGGGYYIQTVPSQIQSVISVYQLLDPEVQRRTSILFLDYKLVSDGYPFPTQLHQLDDTYNKLIQEGNHNITLMGDSAGGHLAIAYTIFLRSLEKLVVYPSKLVLISPWVKLSPLPSDAQEGTSWHDNEDRDLITYHRFSTVSDLKHMIGFEDPFSLLYSPGGKTPRTRDDWSSIPNYSDPNHDVFLILGEDESFRDDIVQWASYALNYPQGEEYGAFSRYKDEAKFTFTRHGQPGNANLTAYVEPMGVHDAMLYFEHDIAKKIEKVLRMKKKPNVKDFDLNQFFGVTRLTKFLNETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA39</accession>
    <name>A0A1A0HA39_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_21022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711264.1">
        <property type="nucleotide sequence ID" value="XM_018855031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA39"/>
    <dbReference type="GeneID" id="30028007"/>
    <dbReference type="OrthoDB" id="5358702at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="223" mass="25102" checksum="49C1F323A3AAE3BE" modified="2016-10-05" version="1" fragment="single">LTRSLYKALTGTSCFYTTVQLLPLGSAVQAVEDRENNTLEIGVPKRVYLQIFAEGHAYFQGSYPRAPDTRRMPRGRLENTYFACLALLATTNDHSTVWRVHELVLAELCRLHHGSWGAADFRFCTALATSRLDRINKSSLLWHWLRKNAVLHVLAARGPAPLYAFIRQILRAMDAHLANCAAGFSLVWLVLVARASGPAFCEEHVALLLRDKCRRTLGDVLLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG9</accession>
    <name>A0A1A0HAG9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4'-phosphopantetheinyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41392</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711382.1">
        <property type="nucleotide sequence ID" value="XM_018857393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAG9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAG9"/>
    <dbReference type="GeneID" id="30030369"/>
    <dbReference type="OrthoDB" id="15433at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="entry name" value="4'-phosphopantetheinyl transferase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="4'-phosphopantetheinyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="4'-phosphopantetheinyl transferase" evidence="4">
        <location>
            <begin position="4"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="127" mass="14315" checksum="5FCD66BF795F3F04" modified="2016-10-05" version="1">MIKAIGVDLVKVLRISRILQGPRRARFLQRVLHDAELTALRKMQSPQKALQYVAGSWAAKEAVFKTLDEREQRQFVFREWRRHHEAGRPGIGRGPSDSGRFLLSISHDGDHLVAMVVRVDPHGATGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAM1</accession>
    <name>A0A1A0HAM1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711684.1">
        <property type="nucleotide sequence ID" value="XM_018854074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAM1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAM1"/>
    <dbReference type="GeneID" id="30027050"/>
    <dbReference type="OrthoDB" id="299997at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="590" mass="66538" checksum="99BC779A8A9E3FDC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAN1</accession>
    <name>A0A1A0HAN1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_42642</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711694.1">
        <property type="nucleotide sequence ID" value="XM_018857565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAN1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAN1"/>
    <dbReference type="GeneID" id="30030541"/>
    <dbReference type="OrthoDB" id="3993315at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="189" mass="22158" checksum="6B07F251E765E42B" modified="2016-10-05" version="1">MSGLRTSEKEHYTVEKFEPDSGISPASPTDNVIKPRASNLLERTESKKPIKFTVRKISREGGMPIEEKGGNLRPITQRYGNLPENKSRAIDRKMGDKAYQLKHNQSKYDEYVTRIDKIEKEVRFLSTLLPPYNVEIDYPTRLKITRAIEKLRSKKDEIEKRKYDLGMSISRLWREFDENNTWVRSVSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAN9</accession>
    <name>A0A1A0HAN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial</fullName>
            <shortName evidence="8">Glu-AdT subunit B</shortName>
            <ecNumber evidence="8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PET112</name>
        <name evidence="10" type="ORF">METBIDRAFT_31590</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Subunit of the heterotrimeric GatFAB amidotransferase (AdT) complex, composed of A, B and F subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711452.1">
        <property type="nucleotide sequence ID" value="XM_018855884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAN9"/>
    <dbReference type="STRING" id="869754.A0A1A0HAN9"/>
    <dbReference type="GeneID" id="30028860"/>
    <dbReference type="OrthoDB" id="1722066at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="GatB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004012">
        <property type="entry name" value="PRK05477.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="9">
        <location>
            <begin position="354"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="519" mass="58509" checksum="CF2A3F7D86F0F45A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAP0</accession>
    <name>A0A1A0HAP0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptide hydrolase</fullName>
            <ecNumber evidence="9">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_78058</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M28 family. M28E subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="9"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711458.1">
        <property type="nucleotide sequence ID" value="XM_018858811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAP0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAP0"/>
    <dbReference type="GeneID" id="30031787"/>
    <dbReference type="OrthoDB" id="2214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000042">
        <property type="entry name" value="Peptide hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF56">
        <property type="entry name" value="AMINOPEPTIDASE YDR415C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012678372" description="Peptide hydrolase" evidence="10">
        <location>
            <begin position="16"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="11">
        <location>
            <begin position="182"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00043962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="401" mass="43599" checksum="72AA9E0056CB2425" modified="2016-10-05" version="1">MKAPVFLALWAAASALSLNFLAGLFSETPDRVIKTGPETYRLASEADKLRLKRNGHKFIDVTGVLSVESALAQGIIAGAGNGDWTHVFAKQIDTLKRPVPAYKYPLAPRQHAVVQALVGRVDLERVRANLARFSGFYTRYYKSASGVKSADWLHARVCELVAPVAGAQVQKIKHRGWDQYSLVVSLPGAAADRVVVGAHQDSINLLFPSLMAAPGADDDGSGTVTSLEALRLVCGAIAAGEFAPHNTLEFHYYSAEEGGLLGSLDVFGRYADANTTVVGMLQQDMTGYTQGTRDGGVEPHFGLITDYTSTRQNDFIRMVAAAYCLIPVHETECGYACLDHAAALENGYPASFLIESEFKYSAKHIHLTMDTMDRLDFDHIREHIKLTVGYAVELAAAQDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAS5</accession>
    <name>A0A1A0HAS5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711492.1">
        <property type="nucleotide sequence ID" value="XM_018854021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAS5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAS5"/>
    <dbReference type="GeneID" id="30026997"/>
    <dbReference type="OrthoDB" id="4092858at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021017">
        <property type="entry name" value="Mediator_Med2_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11214">
        <property type="entry name" value="Med2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="297" mass="33871" checksum="70800F443B6CB16A" modified="2016-10-05" version="1">MNLEEKLTKSLSDILRTSGYVFEIINNNKRQSNLIAGPNNQLIPPPMMEQLAQKIASFELILDDTVAKFNDAPWCVDQIVQNKKKQDEQKIREDRERQQREEAERKRKIEEEALAQKRRAEAEKAARDKQRADQEHRDRLAREQEQREQTAKDSQKAQQHQNQQHQQTRQNAHNPNQQNQAQLLQQYTDHEGMADGPILQNELMDPSFDLDMDIPNPSDILSMNFKDDADPGMGAKEGNPPNTDNPNSDVNGMIKGSDSLLDDLNMDLLGEGFDEGPAGGEEEFDVENFLNQFGHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAZ6</accession>
    <name>A0A1A0HAZ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_173070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711568.1">
        <property type="nucleotide sequence ID" value="XM_018854702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAZ6"/>
    <dbReference type="GeneID" id="30027678"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="129" mass="14662" checksum="C5E16287D7BAAE73" modified="2016-10-05" version="1">MSRAAFWRAAFICGGVRASEFRVRDLAFWMGAGISGAWTKSSLRLPRITSEGAGVICLRGARGLQAFWNEWTGHVEGAQTRKELDSELRRGRGRQEWIEVPGRQWRMQENGSKFQENGSKFQENGSRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBB5</accession>
    <name>A0A1A0HBB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Dihydroorotate dehydrogenase (quinone), mitochondrial</fullName>
            <shortName evidence="11">DHOdehase</shortName>
            <ecNumber evidence="4 11">1.3.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_78295</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>(S)-dihydroorotate + a quinone = orotate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="11">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="4 11"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711790.1">
        <property type="nucleotide sequence ID" value="XM_018858857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBB5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBB5"/>
    <dbReference type="GeneID" id="30031833"/>
    <dbReference type="OrthoDB" id="14784at2759"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106430">
        <property type="term" value="F:dihydroorotate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003652">
        <property type="entry name" value="PRK05286.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF4">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 11" id="KW-0288">FMN</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="102"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="429" mass="46356" checksum="C71E9EAAC3603314" modified="2016-10-05" version="1">MWRFIRPSAKQAVPAARKPLLQASVFPRSILFLAGAGAATAAGIYVLDARAGIHEYVFCPLIRMFTDAETGHKVGVLLMKYGLVPRLPAEDRKTEDGLLAVLGVDVFGTRLRTPIGLAAGLDKDGEAIDSLFDTGFSYVEIGSITPEPQPGNPQPRFFRLPRDDAVINRYGFNSTGHFNVVATLARRFLSNVAEDSQTNAFRDGKLLGINLGKNKAGDEVEDYVKGVSRLGPYADVLIVNVSSPNTPGLRDLQSELKLTSLLTRVVGERERVGQNKLGRKPPVLVKVAPDLTEPEIESIANSAKTAKVDGIIISNTTVQRPLDLYTTDQQLISQAGGLSGKPVKPLSLQALRTLRKYTKDSDLVLIGCGGISSGKDALEFGKAGATFVELYTAFAYKGPGLPAKIRDELAQLLVQEGKTWQQIIGEDDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBN7</accession>
    <name>A0A1A0HBN7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Putative myosin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_31948</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Actin patch</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711936.1">
        <property type="nucleotide sequence ID" value="XM_018855950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBN7"/>
    <dbReference type="STRING" id="869754.A0A1A0HBN7"/>
    <dbReference type="GeneID" id="30028926"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007121">
        <property type="term" value="P:bipolar cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071852">
        <property type="term" value="P:fungal-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11858">
        <property type="entry name" value="SH3_Myosin-I_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000015">
        <property type="entry name" value="Myosin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035535">
        <property type="entry name" value="Fungal_myosin-I_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF837">
        <property type="entry name" value="MYOSIN-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="10 15" id="KW-0518">Myosin</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="18">
        <location>
            <begin position="36"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="19">
        <location>
            <begin position="787"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="1131"/>
            <end position="1191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="15">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="970"/>
            <end position="1036"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1048"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1194"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1255"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="970"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1004"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1057"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="1076"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1212"/>
            <end position="1225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1264"/>
            <end position="1274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="129"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA21426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1274" mass="142083" checksum="4A21C52C422AA076" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBQ9</accession>
    <name>A0A1A0HBQ9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein uL30-like ferredoxin-like fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_41802</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711822.1">
        <property type="nucleotide sequence ID" value="XM_018857453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBQ9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBQ9"/>
    <dbReference type="GeneID" id="30030429"/>
    <dbReference type="OrthoDB" id="509901at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="2">
        <location>
            <begin position="3"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="84" mass="9729" checksum="03DB1363F1404DCC" modified="2016-10-05" version="1">MFYRIQQLRSIIGMPPLVRKNIAALGLRRRNAVVYQRVSVATAHKLRLVKELVKIDLLDELQVQRLKEKESTPYPSGFQKIGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBR2</accession>
    <name>A0A1A0HBR2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_16313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711961.1">
        <property type="nucleotide sequence ID" value="XM_018854634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBR2"/>
    <dbReference type="STRING" id="869754.A0A1A0HBR2"/>
    <dbReference type="GeneID" id="30027610"/>
    <dbReference type="OrthoDB" id="10265467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051314">
        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="450" mass="49387" checksum="B647CDB0F62D7CF3" modified="2016-10-05" version="1" fragment="single">AALAATFTKPLTEVIRPQRFAQYIGQDHLLNSTDGEISNFLRLGYLPSMILCGPPGVGKTSLARLLADEANYVFAEFSATLSTVADVKELLCAIEAENLKRLRQHVSRLRVLVFIDELHRFSTVQQDYLLPFIEKGTFVFVGATTTDAHKRVRRAVLSRCHLFTLVAPADRHLLEVVHRAILYENIRRKVTQKLCFLAYSGLAVQAVVKYASGDMRSAVNLIEILSARLSSAAYRTTGPGGSSPVDAPAVAEAIATLSKARLGLHSEHNLPLLSHLLRCLRYPQSHHTEDIPAAVEGAGGGLLETCPGAGDADSDLESWFDEADRPRYEGEDPKTKAWADHTVFSDDDEAAPGPVYSDDERHVPIESTDLSRHSASKFRVLSATHACLQLFRRGEAPLSILKYLLLFTCTHAFPKKNELIKVLSVLKAVQKAGKGAEQLLLNCVERLAGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS0</accession>
    <name>A0A1A0HBS0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein-serine/threonine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_87552</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711940.1">
        <property type="nucleotide sequence ID" value="XM_018859281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS0"/>
    <dbReference type="GeneID" id="30032256"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005977">
        <property type="term" value="P:glycogen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901525">
        <property type="term" value="P:negative regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="7">
        <location>
            <begin position="28"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="349" mass="39142" checksum="467C0D7BFA374C75" modified="2016-10-05" version="1">MTHAFAGPSTDLDYCLEQLLERRPPRLLPEETVHALCQQLKSQLLGAPNILLLRLPCTVVGDIHGQYHDLLEVFRIGGAPPSTNFVFLGDYVDRGYYSVETMTLLVALKLRYPERSRTITTNYGFYTEILHKYGGSLKVWHHFTELFDFLPLGATIDGRIFATHGGLLPLCQQLDQIRVVDRFKEIPHDGIMADLVWLDPDPDIADFQLLPRGAGYLFGREVVTKFCHDNGLVQLVRAHQLCHEGFISYWQGKCLTIWSAPNYCYRCGNLASVLEISHSSYESRAAGLGPGPAFLPGQFCNVFDASPENAQDTLEGRGVNGVQAPAGPDDVFRAYFDGRSRARQVEYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBT4</accession>
    <name>A0A1A0HBT4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DUF1640-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_78330</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CCDC90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711847.1">
        <property type="nucleotide sequence ID" value="XM_018858865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBT4"/>
    <dbReference type="GeneID" id="30031841"/>
    <dbReference type="OrthoDB" id="889336at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000214">
        <property type="term" value="P:regulation of L-proline metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000018">
        <property type="entry name" value="Mitochondrial protein FMP32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF1">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="192" mass="21665" checksum="96CBD14FAFF5F2C3" modified="2016-10-05" version="1">MNQIGIARRCLSSVTRFDTKKFVQSLEKGGFSPQQAETAVGIVNKAVNDGISLIAKNLVTKERLNSVAYQQKVDFAKLKGELQTMDKSEFTSLKKEQELLRTNLTNLQNRLKEEITKNLAGVRLDLNLEKGRIREESSIHELKIEDTFTRIDEEIANVQMQIKSVKTQVMQWLIGVSSGTAALMLAFVRFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBU5</accession>
    <name>A0A1A0HBU5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leukotriene A(4) hydrolase</fullName>
            <shortName evidence="16">LTA-4 hydrolase</shortName>
            <ecNumber evidence="16">3.3.2.10</ecNumber>
            <ecNumber evidence="16">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_12097</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Aminopeptidase that preferentially cleaves di- and tripeptides. Also has low epoxide hydrolase activity (in vitro). Can hydrolyze the epoxide leukotriene LTA(4) but it forms preferentially 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than the cytokine leukotriene B(4) as the product compared to the homologous mammalian enzyme (in vitro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>an epoxide + H2O = an ethanediol</text>
            <dbReference type="Rhea" id="RHEA:19037"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32955"/>
            <dbReference type="ChEBI" id="CHEBI:140594"/>
            <dbReference type="EC" id="3.3.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 16">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the peptidase M1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.10" evidence="16"/>
    <dbReference type="EC" id="3.4.11.-" evidence="16"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712119.1">
        <property type="nucleotide sequence ID" value="XM_018854166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBU5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBU5"/>
    <dbReference type="GeneID" id="30027142"/>
    <dbReference type="OrthoDB" id="79562at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061957">
        <property type="term" value="C:NVT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120113">
        <property type="term" value="P:cytoplasm to vacuole targeting by the NVT pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000009">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000004">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012777">
        <property type="entry name" value="LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02411">
        <property type="entry name" value="leuko_A4_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 16" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="17">
        <location>
            <begin position="462"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="264"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="573"/>
            <end position="575"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR612777-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM01263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="618" mass="70006" checksum="34CB5F2FBA9F6765" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBY3</accession>
    <name>A0A1A0HBY3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712031.1">
        <property type="nucleotide sequence ID" value="XM_018857339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBY3"/>
    <dbReference type="STRING" id="869754.A0A1A0HBY3"/>
    <dbReference type="GeneID" id="30030315"/>
    <dbReference type="OrthoDB" id="566238at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="64"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="166" mass="18127" checksum="FC72511F6EBA57C9" modified="2016-10-05" version="1">MRRTAAAVPARCLSLAPAKTGFSPAGRRPAALFTRLRAYSVLTQNAAKEYKYRDVKALTEARGGQPGALLIDVREPSEFQEGHIPGARNVPFKSSPGAFDLSEEEFEESFGFEKPAKDQHLVFYCLGGVRLTAAEELANSFGYTNRGNYVGSWEDWVAHENAAGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCJ0</accession>
    <name>A0A1A0HCJ0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ribomal-ubiquitin fusion protein Ubi5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_78544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712148.1">
        <property type="nucleotide sequence ID" value="XM_018858896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCJ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCJ0"/>
    <dbReference type="GeneID" id="30031872"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="17136" checksum="433274C815932066" modified="2016-10-05" version="1">MQIFVKTLTGKTITLEVEASDTIDNVKAKIQDNEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKVYTTPKKIKHKHRKHKLAVLTYYKVDNDGNVERLRRECPTCGAGVFMANHPDRQYCGKCHTTFKLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCJ6</accession>
    <name>A0A1A0HCJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1,3-beta-glucan synthase</fullName>
            <ecNumber evidence="3">2.4.1.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">1,3-beta-D-glucan-UDP glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_229917</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[(1-&gt;3)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;3)-beta-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21476"/>
            <dbReference type="Rhea" id="RHEA-COMP:11146"/>
            <dbReference type="Rhea" id="RHEA-COMP:14303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37671"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.34"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.34" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712225.1">
        <property type="nucleotide sequence ID" value="XM_018855236.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HCJ6"/>
    <dbReference type="GeneID" id="30028212"/>
    <dbReference type="OrthoDB" id="1880850at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000148">
        <property type="term" value="C:1,3-beta-D-glucan synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003843">
        <property type="term" value="F:1,3-beta-D-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006075">
        <property type="term" value="P:(1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051278">
        <property type="term" value="P:fungal-type cell wall polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026899">
        <property type="entry name" value="FKS1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056261">
        <property type="entry name" value="FKS1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003440">
        <property type="entry name" value="Glyco_trans_48_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741:SF97">
        <property type="entry name" value="1,3-BETA-GLUCAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12741">
        <property type="entry name" value="LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14288">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23605">
        <property type="entry name" value="FKS1_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02364">
        <property type="entry name" value="Glucan_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01205">
        <property type="entry name" value="FKS1_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="465"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1176"/>
            <end position="1201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1244"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1330"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1356"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1441"/>
            <end position="1464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1484"/>
            <end position="1509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1521"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1630"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1676"/>
            <end position="1701"/>
        </location>
    </feature>
    <feature type="domain" description="1,3-beta-glucan synthase component FKS1-like" evidence="13">
        <location>
            <begin position="201"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1742" mass="201774" checksum="6D2DEE7195D4D682" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCJ7</accession>
    <name>A0A1A0HCJ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712216.1">
        <property type="nucleotide sequence ID" value="XM_018854186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCJ7"/>
    <dbReference type="GeneID" id="30027162"/>
    <dbReference type="OrthoDB" id="4089215at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="399" mass="44750" checksum="6D48C62343F11BAC" modified="2016-10-05" version="1">MRSYNASERIPDFELFQTHQISVPLERTKYLFMSMFLSRALNLKDESSILDPASYKAKTHVSLVEILPHIAIPSDLWLLSEIGIPHLKVKVALLKDINAMLARVLDSLNNGEYFMLDDHLLRSFETRVAEYRMTELSPDFTAVQSEEPQIEDTSELDHVSELSLSIKDYPADTDTMSVSSASTRTGSMSKNSNRMSTFSRDLLLGKRKLSIFGLNQHLKPVLLPPNEDRVSGNPGRTLQISPETPPSEPSNTHQFVTQKSSQMNGLLLKSKFYNKLVKRRDLEVADPASLSASSTPSYLSLVGAPGQGDTDGYSSRRFLVSTTQRIENQKDKYHFYSQTKTLGENIEALVAGFGRRGSQANLVTLMEFIKNHVFKFVVADICHMIIAKAQAGLVRDILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD06</accession>
    <name>A0A1A0HD06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">pyridoxal 5'-phosphate synthase</fullName>
            <ecNumber evidence="4">1.4.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_77387</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712319.1">
        <property type="nucleotide sequence ID" value="XM_018858688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD06"/>
    <dbReference type="STRING" id="869754.A0A1A0HD06"/>
    <dbReference type="GeneID" id="30031664"/>
    <dbReference type="OrthoDB" id="303614at2759"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004231">
        <property type="entry name" value="PRK05679.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851:SF0">
        <property type="entry name" value="PYRIDOXINE-5'-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PYRIDOXINE-5-PHOSPHATE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="PNPOx_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine 5'-phosphate oxidase N-terminal" evidence="8">
        <location>
            <begin position="54"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridoxine 5'-phosphate oxidase dimerisation C-terminal" evidence="9">
        <location>
            <begin position="186"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="228" mass="26218" checksum="AD1276EB7A0482E8" modified="2016-10-05" version="1">MAAEKPLIFAPPTYQYEKGQLNEADINSNPLTQFHKWFHEAQQTLPEGLSAIPESVVFSTARLPEGRISSRVVLLKELDPHGFIVYSNWGTSKKSKDYSTNKHASLTFFWSHIQRQVRVEGVMEKVTRETSERYFKTRPRGLKIGAWASPQSEVIQSRGELDVLYKSYEEKFADLADDEIPCPDFWGGVRIVPLEIEFWQGGVSRLHDRLTFTREKTDGEWAVKRISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDI2</accession>
    <name>A0A1A0HDI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_21709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBCC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712572.1">
        <property type="nucleotide sequence ID" value="XM_018855095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDI2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDI2"/>
    <dbReference type="GeneID" id="30028071"/>
    <dbReference type="OrthoDB" id="194775at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027684">
        <property type="entry name" value="TBCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139">
        <property type="entry name" value="TUBULIN FOLDING COFACTOR C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15139:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="109" mass="12437" checksum="426A64EF0030071F" modified="2016-10-05" version="1" fragment="single">DELPWSIHAQGLKSCLVAIRCDGPIILHDVENLVLILECHQLRIHNMRNCQVYALVANDRVIIEDSRDLIFLGFSEDALGPPCFVVDDFDWPTSETVNPHFKMKTFSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDU9</accession>
    <name>A0A1A0HDU9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphate permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77641</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712677.1">
        <property type="nucleotide sequence ID" value="XM_018858737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDU9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDU9"/>
    <dbReference type="GeneID" id="30031713"/>
    <dbReference type="OrthoDB" id="433512at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097079">
        <property type="term" value="F:selenite:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097080">
        <property type="term" value="P:plasma membrane selenite transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17364">
        <property type="entry name" value="MFS_PhT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000421">
        <property type="entry name" value="Inorganic phosphate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004738">
        <property type="entry name" value="Phos_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00887">
        <property type="entry name" value="2A0109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="55"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="59796" checksum="B2E7A85BAA2F27EC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE76</accession>
    <name>A0A1A0HE76_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit beta</fullName>
            <ecNumber evidence="14">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_38794</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATP synthases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha/ATP1 and beta/ATP2 form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha/ATP1(3)beta/ATP2(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta/ATP2 subunits.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="14"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712792.1">
        <property type="nucleotide sequence ID" value="XM_018857049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE76"/>
    <dbReference type="STRING" id="869754.A0A1A0HE76"/>
    <dbReference type="GeneID" id="30030025"/>
    <dbReference type="OrthoDB" id="14523at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000005">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="174"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA22296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="503" mass="53938" checksum="F15E05795886AE22" modified="2016-10-05" version="1">MVLPRLFNTASRGAFTAAKRNVLFNRSLATAAAANGKVRAVIGAVVDVQFEEGNLPAIFNALTLKNGQQSLVLEVAQHLGENTVRTIAMDGTEGLVRGAAVHDTGAPISVPVGRETLGRIINVVGEPIDERGPINTKKRNPIHTDPPSFVQQSTSAEVLETGIKVVDLLAPYARGGKIGLFGGAGVGKTVFIQELINNIAKAHGGFSVFTGVGERTREGNDLYREMQETGVINFEGDSKVALVFGQMNEPPGARARVALTGLTIAEYFRDEEGQDVLLFVDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGALQERITTTKKGSVTSVQAVYVPADDLTDPAPATTFAHLDATTVLSRGISELGIYPAVDPLDSNSRLLDATVVGQEHYDVATNVQQTLQAYKSLQDIIAILGMDELSETDKLTVERARKIQKFLSQPFAVAEVFTGIEGRLVRLEDTVRSFKEVLDGKYDHLPENAFYMVGGIEDAVAKAEKLAAESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEC1</accession>
    <name>A0A1A0HEC1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712831.1">
        <property type="nucleotide sequence ID" value="XM_018857153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEC1"/>
    <dbReference type="STRING" id="869754.A0A1A0HEC1"/>
    <dbReference type="GeneID" id="30030129"/>
    <dbReference type="OrthoDB" id="5421784at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="133" mass="14775" checksum="3BAFC7179A465788" modified="2016-10-05" version="1" fragment="single">MKNSSSQGRYTPVAHTLLLPRSLDENQAFFDAEPASSLLAAGTLSQAPDSQPNPESRPNPESRPYPESQPYPESQPNPESQPNPESQPNPESQPYPESQPYPESQPNPESQPYPELQPYPESQPYPELQPLAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HED4</accession>
    <name>A0A1A0HED4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_31188</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712758.1">
        <property type="nucleotide sequence ID" value="XM_018855799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HED4"/>
    <dbReference type="STRING" id="869754.A0A1A0HED4"/>
    <dbReference type="GeneID" id="30028775"/>
    <dbReference type="OrthoDB" id="550575at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09293">
        <property type="entry name" value="AMN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057207">
        <property type="entry name" value="FBXL15_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382:SF67">
        <property type="entry name" value="SCF E3 UBIQUITIN LIGASE COMPLEX F-BOX PROTEIN POF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25372">
        <property type="entry name" value="DUF7885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box/LRR-repeat protein 15-like leucin rich repeat" evidence="3">
        <location>
            <begin position="345"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="41"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="97"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="557" mass="62795" checksum="03E205364DCCD754" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEN3</accession>
    <name>A0A1A0HEN3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Chromatin remodelling complex ATPase chain ISW1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_39133</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712957.1">
        <property type="nucleotide sequence ID" value="XM_018857097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEN3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEN3"/>
    <dbReference type="GeneID" id="30030073"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008623">
        <property type="term" value="C:CHRAC"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005880">
        <property type="term" value="C:nuclear microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019237">
        <property type="term" value="F:centromeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071444">
        <property type="term" value="P:cellular response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060195">
        <property type="term" value="P:negative regulation of antisense RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000002">
        <property type="entry name" value="ISWI chromatin-remodeling complex ATPase CHR11 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="157"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="456"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="15">
        <location>
            <begin position="828"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1000"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1012"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1047"/>
            <end position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA22461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1056" mass="120616" checksum="51FACD7B107D152C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEU0</accession>
    <name>A0A1A0HEU0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">U3 snoRNP protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_40745</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713012.1">
        <property type="nucleotide sequence ID" value="XM_018857305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEU0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEU0"/>
    <dbReference type="GeneID" id="30030281"/>
    <dbReference type="OrthoDB" id="10250769at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="5">
        <location>
            <begin position="49"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="591"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="4">
        <location>
            <begin position="721"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="929" mass="100086" checksum="0B8EF007DE6A3379" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEV5</accession>
    <name>A0A1A0HEV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_39638</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713027.1">
        <property type="nucleotide sequence ID" value="XM_018857160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEV5"/>
    <dbReference type="GeneID" id="30030136"/>
    <dbReference type="OrthoDB" id="269919at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000008">
        <property type="entry name" value="Translation initiation factor 2B, beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051855">
        <property type="entry name" value="eIF2B_beta_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45859:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="362" mass="40064" checksum="2B98162844451FEF" modified="2016-10-05" version="1">MPSALTLDVQALVDPLVLELKRKQHTGDKDVALKVAHVLMRVISAARWTDPHDLIALIRKVGLDLHHASPREFIPGNVVRRVLAIIRDEIETADPLAQDNTPMISLMFSLLSTAHSVQDTQHPQHKLLKKQLSDMRSIVIQGIRDLIDEISNVKEELENMAADLIHENELLLTATPGSDTVLQFLIKARAKRNFTVLVTESFPNDTEDAHRFVKALAENKIQTVLIPDSTVFAVMPRVGKVILGANTVFANGGCMLNSGVANVVECAKEHKTPVFGVAGLYKLSPLYPFNREDLIEVGNSGKVLSYSNSKLMENVDVVTNPLGDYVPPEHIDIYITNVGGFAPSFIYRIVLDNYKPEDTSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEX7</accession>
    <name>A0A1A0HEX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_77851</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712959.1">
        <property type="nucleotide sequence ID" value="XM_018858780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEX7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEX7"/>
    <dbReference type="GeneID" id="30031756"/>
    <dbReference type="OrthoDB" id="67566at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021149">
        <property type="entry name" value="OligosaccharylTrfase_OST3/OST6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692:SF3">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12692">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04756">
        <property type="entry name" value="OST3_OST6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="340" mass="38467" checksum="B00919F6BE351750" modified="2016-10-05" version="1">MASQCGNSYTVRALHSPDFIVPVENSNLTLLSQPQRGHFTLILITSTSEQHGCESCHAIRKVLGKVAQAWTADYLHLGYLYFAEIDLVDRGNSEVFNFLGLKTVPQLWLVPPLSVASKHTLQRQPKIDEYGLEYFDNFDILLEPHAEFKLPEAAFDDQVFAMADWLAKAVQKRITIRQENASTKFIATFLATFLGILIIKKRGPSLITQTVSKKRIYKLAFFAFILVLLGGFLFSLMLKVPFIAKNDNNEPIYISGGTLWQFGVEMVLVGGTYGLLGATLIMLVYLGSYEIGETSMIQSESQHGLLVLFVAGMLYFLYSVLTSMYLRKDEWYPYYFARLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF01</accession>
    <name>A0A1A0HF01_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_35585</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713185.1">
        <property type="nucleotide sequence ID" value="XM_018856630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF01"/>
    <dbReference type="STRING" id="869754.A0A1A0HF01"/>
    <dbReference type="GeneID" id="30029606"/>
    <dbReference type="OrthoDB" id="2102561at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000140">
        <property type="term" value="F:acylglycerone-phosphate reductase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019433">
        <property type="term" value="P:triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44169:SF6">
        <property type="entry name" value="NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="289" mass="30786" checksum="EB6CD7C57E138FB6" modified="2016-10-05" version="1">MARQKVVLVTGASSGIGLAAAVEFAKRGYKVYACARRLAPMAPLEAHGIHTLVCDVTQTDAVARMCSHISSDNDGRLDILYNNAGQACTMAAMDATDAAVAQCFAVNVFAPMAVTREFAALLIKARGTVGFTGSVSGVLPAPFLAVYSASKAALHQYVATLRVEMEPFGVTVLNFVTGSVQTPIGDLRGLPEHSRFRVPGIDAAMAELLGMTALKRPVSAELYARRVVGDFEAQRLGGRLHIYRGGAAYFAGHLLAWCPRVLAEKLLVSQLKFSEAFEYIRARHLGAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF29</accession>
    <name>A0A1A0HF29_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Dipeptidyl peptidase 3</fullName>
            <ecNumber evidence="4 15">3.4.14.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 15">Dipeptidyl aminopeptidase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 15">Dipeptidyl peptidase III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_39009</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.</text>
            <dbReference type="EC" id="3.4.14.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the peptidase M49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.14.4" evidence="4 15"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712989.1">
        <property type="nucleotide sequence ID" value="XM_018857078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF29"/>
    <dbReference type="STRING" id="869754.A0A1A0HF29"/>
    <dbReference type="GeneID" id="30030054"/>
    <dbReference type="OrthoDB" id="4694525at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.540.30:FF:000002">
        <property type="entry name" value="Dipeptidyl peptidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.30">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005317">
        <property type="entry name" value="Dipeptidyl-peptase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039461">
        <property type="entry name" value="Peptidase_M49"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422:SF11">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23422">
        <property type="entry name" value="DIPEPTIDYL PEPTIDASE III-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03571">
        <property type="entry name" value="Peptidase_M49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007828">
        <property type="entry name" value="Dipeptidyl-peptidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 17" id="KW-0862">Zinc</keyword>
    <feature type="active site" evidence="16">
        <location>
            <position position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="455"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR007828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR007828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA22493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="695" mass="76800" checksum="8A7A4CBA0FB989E3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF48</accession>
    <name>A0A1A0HF48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713122.1">
        <property type="nucleotide sequence ID" value="XM_018853985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF48"/>
    <dbReference type="GeneID" id="30026961"/>
    <dbReference type="OrthoDB" id="4081922at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="38378" checksum="49DE44495ACC3285" modified="2016-10-05" version="1">MDIKKSYEYWQELVSKNMKLRLKPRRASDAGGGAEISNDQLRERVPPYREPTLRDLMTSEELELFSKPELQSFRQKVSEKLALQRGLQLKDSDVDKLLESCLADLQFSQGFDPYGNEDEVGLHDGNSCTQDLALNTYEGPGESDEDVLADDYHMMGPSRHRHFEVELSDGPPGIEPDPNEPSCEFTFEYDRDGKLIPTSNNIEEKLRLMNLQSQITNEALSQALALAMAMAAGGKKKKKHNKKKKKAKGEEDHGTCVETRSDLCLFCQYETFYGVKPLYSMRKLDYTHKWEMERRMKFREKLGAAKLYKAKHEHSRDDAVLVDDLEDSHGTVEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF66</accession>
    <name>A0A1A0HF66_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_154891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713024.1">
        <property type="nucleotide sequence ID" value="XM_018854603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF66"/>
    <dbReference type="GeneID" id="30027579"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="166" mass="17569" checksum="0F0A890F8B35D1C9" modified="2016-10-05" version="1">MAGQSPRRGSGALYVLLLRLPSGERAGIAHFSGVLHAAPGSGSDGSFDAPRFCGYGGVWLRQAGILAGNCTESRLIGVLVAVLSALAWWPGGPYWRRRSRDAQGHARADPPLQFTNEWVGSICVSLFLCVSLSFCSLSFCVCLPLVGSNVPDGQTFISCECATSYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFE1</accession>
    <name>A0A1A0HFE1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Cystathionine beta-synthase</fullName>
            <ecNumber evidence="4 9">4.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37326</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>L-homocysteine + L-serine = L,L-cystathionine + H2O</text>
            <dbReference type="Rhea" id="RHEA:10112"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="4.2.1.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.22" evidence="4 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713345.1">
        <property type="nucleotide sequence ID" value="XM_018856858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFE1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFE1"/>
    <dbReference type="GeneID" id="30029834"/>
    <dbReference type="OrthoDB" id="728at2759"/>
    <dbReference type="UniPathway" id="UPA00136"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004122">
        <property type="term" value="F:cystathionine beta-synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019343">
        <property type="term" value="P:cysteine biosynthetic process via cystathionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070814">
        <property type="term" value="P:hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007089">
        <property type="term" value="P:traversing start control point of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000003">
        <property type="entry name" value="Cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000118">
        <property type="entry name" value="Related to CYS4-cystathionine beta-synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005857">
        <property type="entry name" value="Cysta_beta_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01137">
        <property type="entry name" value="cysta_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0129">CBS domain</keyword>
    <keyword evidence="9" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="10">
        <location>
            <begin position="352"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="493" mass="54001" checksum="8149CDED8465E4BE" modified="2016-10-05" version="1">MSVPPISNDVLDLIGNTPLIRLNKIPQSYGIKAKVYAKVELFNLGGSIKDRIAKNMVLEAEKSGRIKPGYTLIEPTSGNTGIGLALVGAVRGYRTIITLPEKMSNEKVSVLKALGAEIIRTPTEAAWDAPESHIGVARKLEKEIPNSVILDQYGNEANPDAHYYGTGFEIWEQTEGKITHLVAGAGTGGTISGISRYLKEQNPEVTVHGADPKGSILAQPESLNASTDGYLVEGIGYDFIPDVLDRKYVDHWIKTDDAESFKLARRIIREEGILVGGSSGSALQAALQVGKDLTEEDTLVVVFPDSIRSYLSKFADDEWMKTNGFKLEENNVVADKTDDFMNNRSIKDLVAGKPPVVTVTLADTVEKTFNLLQSNGFDQLPVLNESGKLLGLITLSQILKALSTKKVQMTNSIRSIFLDFSKLADFEKSFSITKKSGFTKRSYQTITLDTSLSLLNKFFETNSNAIITDKEMKPVQIVTKVDLLAYMAKEVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFE3</accession>
    <name>A0A1A0HFE3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">candidapepsin</fullName>
            <ecNumber evidence="4">3.4.23.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_54737</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.</text>
            <dbReference type="EC" id="3.4.23.24"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase A1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.24" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713084.1">
        <property type="nucleotide sequence ID" value="XM_018858292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFE3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFE3"/>
    <dbReference type="GeneID" id="30031268"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05474">
        <property type="entry name" value="SAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000011">
        <property type="entry name" value="Aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033876">
        <property type="entry name" value="SAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF65">
        <property type="entry name" value="ASPARTIC-TYPE ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 17" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013289163" description="candidapepsin" evidence="15">
        <location>
            <begin position="16"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="451"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="16">
        <location>
            <begin position="68"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="474" mass="51249" checksum="8AA795B82ABFB4DA" modified="2016-10-05" version="1">MISLSYLALVALASAASCPKIPKASTLKMEFLISKANSRASSMEGAAVELVKKDDMQELALINHFTFYSTTLRVGPQGAEIDVLIDTGSSDLWVMSSEIQCLTTPDSQSSCAQVDSFATADSDSFKVNSTAPEFHISYLSNTYAKGYWGHDYVAFGEIRIPDCSFAVVNETTSRHSVFGIGLAQSESTYSGINRRSTPYTYENFPLRLKSTGLINKVLYSLYLNASDALTGSILFGAVDHARYTGQLHTVPLVNINAQYFAKPIRFDIILDSITLESTSHNVSVFLSQVLALLDSGASLTYLPGAILDGFASLLGAYMNSSEAYTINCEKASDRIFTVFYFSGARIKVPLSDLIFRLGSSCYLGILELPSDQLSGASYAILGDNFLRSAYVVYDLEEYEISLAQAFHGSEENIEVVTSSIPLATRNGGPLSRSLANMSSSSQLTPTGQKQFTGTISFATSVLLAVFGVCVLLAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFF2</accession>
    <name>A0A1A0HFF2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713123.1">
        <property type="nucleotide sequence ID" value="XM_018853986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFF2"/>
    <dbReference type="GeneID" id="30026962"/>
    <dbReference type="OrthoDB" id="4074293at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="120" mass="13202" checksum="700DC1287CC47332" modified="2016-10-05" version="1">MLAKSATPATPVTRPVSHIGDSTTWIKQWYTPLENSSSSSVSNCKLRGWVKTTNAPLEQAIAISSDTYDLDKHSSHQEPEEEKPVVEETPVEQTENSDLKLALSMEKREDQVSSLSGLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFF5</accession>
    <name>A0A1A0HFF5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_91428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713212.1">
        <property type="nucleotide sequence ID" value="XM_018859324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFF5"/>
    <dbReference type="GeneID" id="30032299"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="19472" checksum="586779FDEB41CFF6" modified="2016-10-05" version="1">MQGSAGFLVRAGSGVFLEAGQAGPWGRARWRGCTVVSMLRPPRRNPSSGFEAPAPMRWICTISGRAARLRTSSVSGKHAGARASVWDALAAGGFLASYICRPKRPVKRLSPGARHVPHGPRSPGAVLAVWFVREAAWCVYFCLGPRGLLPDLCFVHGLCLSLNALYYSEFCYATPGPVMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM3</accession>
    <name>A0A1A0HFM3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_92825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713282.1">
        <property type="nucleotide sequence ID" value="XM_018859349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM3"/>
    <dbReference type="GeneID" id="30032324"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="112" mass="12826" checksum="E8C0860FBB2B5DB9" modified="2016-10-05" version="1">MIFCFFQSSFFSMIFCFSHFHISSLFYMSLSSTLILLYPIHINYIATFPCPHSHIPILIIQYIAPSSVLLQLYLHVPASASSSSSVYIDTSHRHPIITFSLSTRRCILIFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG06</accession>
    <name>A0A1A0HG06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mtf2-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713269.1">
        <property type="nucleotide sequence ID" value="XM_018856873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG06"/>
    <dbReference type="STRING" id="869754.A0A1A0HG06"/>
    <dbReference type="GeneID" id="30029849"/>
    <dbReference type="OrthoDB" id="4084661at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043837">
        <property type="entry name" value="Mtf2-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19189">
        <property type="entry name" value="Mtf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mtf2-like C-terminal" evidence="1">
        <location>
            <begin position="70"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="33933" checksum="B264130420FA1B2E" modified="2016-10-05" version="1" fragment="single">VADEKDLFEKIFATYAQSEASSTAADIVQRRVLANLQDSVRAPAAAADHVPVALATERLSGREQGVAERKTVEALAPTLEYIDQMTTREAVVGFTRRVLEGCVAGVAQRRDFHLHKAKGETSKAFLTRHARLSRQVRAQSEASPGAPVLNAFTVPVLFNHVLRAVAARLHDGALALSLFNRVKTDLRLYSVVCNQATYNEMLKVHWVFFGKRSLCEIELLVVEMQNNGFAGDGQTFVVLLKILAAYHAMKAGRSSFSPGGLPVWAHEDDKRVRNLAAKLQVLGKLLERERLLATADRSARCSRHALGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG41</accession>
    <name>A0A1A0HG41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Succinate-semialdehyde dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="9">1.2.1.16</ecNumber>
            <ecNumber evidence="3">1.2.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">NAD(+)-dependent succinic semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_37105</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="9"/>
    <dbReference type="EC" id="1.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713304.1">
        <property type="nucleotide sequence ID" value="XM_018856821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG41"/>
    <dbReference type="STRING" id="869754.A0A1A0HG41"/>
    <dbReference type="GeneID" id="30029797"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF5">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="56"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="509" mass="54073" checksum="FA358A1CBE7EEE25" modified="2016-10-05" version="1">MIVSSVRRYSNASPGISARLSQLVKNQGLLRLQAYINGEWVSSAATFGVRDPGRVPHKSAHLADVSSHPKAHFARAIESAQHAFRLYRTTTPRQRADLLQRMHRLMVDNKTDLATIISLENGKPLADAAGEVLYAASFFQWFGEEAAHTTGDIISSSNAASRILAIRQPVGVCGIITPWNFPAAMITRKLAAALSVGCTAVVKPASETPLTALALAHLAHEAGFAKGVVNIVPSADSAEAGRAICEHELVKKVTFTGSTAVGKALMQQAAATVKKCSFELGGNAPFLVFEDARVDDAVAGAVASAFRSSGQTCICANRIFVHESVYAEFAQKLVRALRSATTLGHGLASGTTHGPLIHGRAMDKVARHVQDAQALGADLLLGGRPRRELGDLYHELTVLGNVTPDMAVFRDETFGPVCPLIKFASEDEAVALANDTPVGLAGYFYTRDMRRVFRVAEHLEVGMVGVNTGGISEASLPFGGVKESGFGREGSKYGADEYTVVKSIVLGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGE4</accession>
    <name>A0A1A0HGE4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_98232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713557.1">
        <property type="nucleotide sequence ID" value="XM_018859467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGE4"/>
    <dbReference type="GeneID" id="30032442"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="82" mass="8805" checksum="5D04F61D3B6D9551" modified="2016-10-05" version="1">MLSSVPTVFTVMSPFCTLSWTCFSFMSTVLLLLGLLPVPLTDKTTPLLSSSMVGTSIVSRLKSLQSAFNHNTSFHAIGVPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGE9</accession>
    <name>A0A1A0HGE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LTV-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LTV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713562.1">
        <property type="nucleotide sequence ID" value="XM_018859376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGE9"/>
    <dbReference type="STRING" id="869754.A0A1A0HGE9"/>
    <dbReference type="GeneID" id="30032351"/>
    <dbReference type="OrthoDB" id="5852896at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007307">
        <property type="entry name" value="Ltv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531">
        <property type="entry name" value="LOW-TEMPERATURE VIABILITY PROTEIN LTV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21531:SF0">
        <property type="entry name" value="PROTEIN LTV1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04180">
        <property type="entry name" value="LTV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="479"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="618"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="490"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="519"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="618"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="638"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="658" mass="71336" checksum="B0BD1BBD25CAF4E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH69</accession>
    <name>A0A1A0HH69_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">26S proteasome regulatory subunit RPN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76377</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713827.1">
        <property type="nucleotide sequence ID" value="XM_018858520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH69"/>
    <dbReference type="STRING" id="869754.A0A1A0HH69"/>
    <dbReference type="GeneID" id="30031496"/>
    <dbReference type="OrthoDB" id="261572at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000017">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048570">
        <property type="entry name" value="PSMD1_RPN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040623">
        <property type="entry name" value="RPN2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18004">
        <property type="entry name" value="RPN2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21505">
        <property type="entry name" value="RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit 1/RPN2 N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome regulatory subunit RPN2 C-terminal" evidence="8">
        <location>
            <begin position="758"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="813"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="826"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="837"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="962" mass="104971" checksum="F977674ED76348C0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH78</accession>
    <name>A0A1A0HH78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-activating enzyme E1 1</fullName>
            <ecNumber evidence="5">6.2.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_34070</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.45" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714015.1">
        <property type="nucleotide sequence ID" value="XM_018856437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH78"/>
    <dbReference type="STRING" id="869754.A0A1A0HH78"/>
    <dbReference type="GeneID" id="30029413"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004839">
        <property type="term" value="F:ubiquitin activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01491">
        <property type="entry name" value="Ube1_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01490">
        <property type="entry name" value="Ube1_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2660:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12550:FF:000001">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000015">
        <property type="entry name" value="Ubiquitin-activating enzyme E1 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.180:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.60:FF:000002">
        <property type="entry name" value="Ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12550">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.60">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, UFD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032420">
        <property type="entry name" value="E1_4HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032418">
        <property type="entry name" value="E1_FCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038252">
        <property type="entry name" value="UBA_E1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018075">
        <property type="entry name" value="UBQ-activ_enz_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01408">
        <property type="entry name" value="Ube1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16191">
        <property type="entry name" value="E1_4HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16190">
        <property type="entry name" value="E1_FCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09358">
        <property type="entry name" value="E1_UFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="14">
        <location>
            <begin position="888"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1018" mass="112220" checksum="87747414BF075E52" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHC0</accession>
    <name>A0A1A0HHC0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_103207</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713753.1">
        <property type="nucleotide sequence ID" value="XM_018853735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHC0"/>
    <dbReference type="GeneID" id="30026711"/>
    <dbReference type="OrthoDB" id="3993307at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="346"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="372" mass="41956" checksum="F6F4800C7A1EF0EC" modified="2016-10-05" version="1">MEKLPSKVLSPRNRNISQSTGLAKFKLSPKRAHLESTKDACALLYEPPKLSHQFNAELERKASFHRARSVFDGSPLRKCGEKQAEFPDIPGLLSTRPKAKSPFRPSFTVERATGNTIKEQKSVKEPSRNDLLFKYAEKQTKLLELQRQKEVLEFEILEIEAQLQKNIGQDTFAKVHTEPNTLKNKVSTLFQSGPDSSLLRKTASTIFSTNTEISKESTPFFSPKLSAPRQNSPVASLRKTASSIFTPPANFMPNHELKKKASAMLNNKFMNDVRDKIDQQQNEFEEFTKKGTEFARNFITSLSPKKEPEPRESILADSSFSVDNLVNLSIADRSVLLSDDEYEGENILDDSEQSAIHIDDCDSDDSYAADEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHW9</accession>
    <name>A0A1A0HHW9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_30043</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714086.1">
        <property type="nucleotide sequence ID" value="XM_018855541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHW9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHW9"/>
    <dbReference type="GeneID" id="30028517"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000006">
        <property type="entry name" value="General amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF15">
        <property type="entry name" value="GENERAL AMINO ACID PERMEASE AGP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="286"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="464"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="494"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="53"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="562" mass="62435" checksum="5AAEB74166CD451B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI60</accession>
    <name>A0A1A0HI60_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_110556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714167.1">
        <property type="nucleotide sequence ID" value="XM_018853910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI60"/>
    <dbReference type="GeneID" id="30026886"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="130" mass="15664" checksum="ECEF0D4514D5B393" modified="2016-10-05" version="1">MFYFCGSRIDFKSRKFQLKQTSFQTHLSFTKMRYLKIWKWHWVCQHKTLEQNRASNLGLVDSCSYFLIQPCGMCGCIITSQSTYFLFMRVRDPGEYLAAIVVFLYCFVQWLSFGKFEEYFADRRLRQASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIK9</accession>
    <name>A0A1A0HIK9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial group I intron splicing factor CCM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PPR family. P subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714470.1">
        <property type="nucleotide sequence ID" value="XM_018853703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIK9"/>
    <dbReference type="STRING" id="869754.A0A1A0HIK9"/>
    <dbReference type="GeneID" id="30026679"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050872">
        <property type="entry name" value="PPR_P_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128">
        <property type="entry name" value="MITOCHONDRIAL GROUP I INTRON SPLICING FACTOR CCM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46128:SF211">
        <property type="entry name" value="PENTACOTRIPEPTIDE-REPEAT REGION OF PRORP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="757" mass="83694" checksum="B9550AA43B840E06" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIP3</accession>
    <name>A0A1A0HIP3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_81264</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Eukaryotic pyruvate dehydrogenase (PDH) complexes are organized as a core consisting of the oligomeric dihydrolipoamide acetyl-transferase (E2), around which are arranged multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3) and protein X (E3BP) bound by non-covalent bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714361.1">
        <property type="nucleotide sequence ID" value="XM_018859209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIP3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIP3"/>
    <dbReference type="GeneID" id="30032185"/>
    <dbReference type="OrthoDB" id="202158at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004742">
        <property type="term" value="F:dihydrolipoyllysine-residue acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000010">
        <property type="entry name" value="Acetyltransferase component of pyruvate dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="entry name" value="E3-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045257">
        <property type="entry name" value="E2/Pdx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151">
        <property type="entry name" value="DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23151:SF82">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE COMPLEX PROTEIN X COMPONENT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="6">
        <location>
            <begin position="172"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="7">
        <location>
            <begin position="340"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="30"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="286"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="60433" checksum="87823ACF5403B51B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIT4</accession>
    <name>A0A1A0HIT4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="3 10">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37590</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="3 10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714278.1">
        <property type="nucleotide sequence ID" value="XM_018856894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIT4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIT4"/>
    <dbReference type="GeneID" id="30029870"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025770">
        <property type="entry name" value="PPMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51564">
        <property type="entry name" value="SAM_ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="213" mass="23786" checksum="566C37DD1FB3AA8E" modified="2016-10-05" version="1">MSAVSMQVALLVTVGLASLYSIVWGGNSRLAKYTLAWACFHLAEYGCTKRYLPRTVTPHLFLIWGARGAVPLAAVHLASICEYLLTRKYGRHHGLPACGVLLAAAGIFVRGKAIHDCGNSFSHYIETETPQRLVTSGIYAWCRHPSYAGFLLYVVGMQTILGNVLVHALSLVVLVRFFGRRIQIEEHFLVARFYGARYEEYRARVCALVPYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIV4</accession>
    <name>A0A1A0HIV4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_35385</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714570.1">
        <property type="nucleotide sequence ID" value="XM_018856604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIV4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIV4"/>
    <dbReference type="GeneID" id="30029580"/>
    <dbReference type="OrthoDB" id="3364892at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF26">
        <property type="entry name" value="CARRIER PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G07710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="135"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="393" mass="44695" checksum="EF0801F3DA1E8F78" modified="2016-10-05" version="1">MSAENLRNEPESKSNYQSVSLSTAGMRALIFQLQSIYLRAPLKLFRPSRFDYMIYVREQANKHTEIFSKPYKFRTHSSLGMLISVMRREGWRFIPDNVLPPLLANSATGVILYGTYLGALDMFSSHSGPKTDFAYSPVDTWRAGFLAGAMQSLAAAPIDAIYSRLTTAEMLSGKHENLWKYGAHKLKEIGLVGVFAGYAFSIVKESFGFAFYFLTFELIKTRGYNLTYRVISTYRNFKVTLRDKLSFLSSLEPKVNSAQIKKEQSRLTKILKSSFVLLAGASAAFSLLAVQYPLTKVQKIHYNRLEALDIYSSSVHPLKKRPFVKVYWNSYLDTFQQVMKNKQTSRMSWFQLAYKGFARNALTTIPATSVGLLIFEIMRTRLADDFEEAGPLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ10</accession>
    <name>A0A1A0HJ10_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Mannan endo-1,6-alpha-mannosidase</fullName>
            <ecNumber evidence="4 11">3.2.1.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_34643</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Random hydrolysis of (1-&gt;6)-alpha-D-mannosidic linkages in unbranched (1-&gt;6)-mannans.</text>
            <dbReference type="EC" id="3.2.1.101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glycosyl hydrolase 76 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.101" evidence="4 11"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714625.1">
        <property type="nucleotide sequence ID" value="XM_018856505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ10"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ10"/>
    <dbReference type="GeneID" id="30029481"/>
    <dbReference type="OrthoDB" id="4187847at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008496">
        <property type="term" value="F:mannan endo-1,6-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007117">
        <property type="term" value="P:budding cell bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016052">
        <property type="term" value="P:carbohydrate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000006">
        <property type="entry name" value="Mannan endo-1,6-alpha-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005198">
        <property type="entry name" value="Glyco_hydro_76"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014480">
        <property type="entry name" value="Mannan-1_6-alpha_mannosidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12145:SF36">
        <property type="entry name" value="MANNAN ENDO-1,6-ALPHA-MANNOSIDASE DCW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03663">
        <property type="entry name" value="Glyco_hydro_76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016302">
        <property type="entry name" value="Man_a_manosd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291853" description="Mannan endo-1,6-alpha-mannosidase" evidence="13">
        <location>
            <begin position="22"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="432"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="454" mass="50422" checksum="38B3DFCF4B4F3995" modified="2016-10-05" version="1">MMLGLLKMVPASFLWLSSVLALSLDVNSDESVCAAAKEIVQGEMNYYEGITYGGTVGMFSAPYYWWHAGEVFGGWVDYWAYCASDNDTFTEILYDAMYAQKGDDYNYMPLNQSLTEGNDDQGVWGMAIMQAVERNFTNPTDHSWLYMIQAIFNSMNNRWDTTSCNGGLRWQIFTWNNGYTYKNSIANGCLFHIAARLYRYTGEDLYLEVAEKVYDWMWDVGYFVEEDGELIIYDGAETTENCTDLTMYEWSYTYGIFMSGYAYLYNATGETKWKTAAQDVIDASSYFFNGSIMTETTCAYSDVCNTDQRSFRSLFSRCLGLTATLIPDTYDTIFTGWLVPSAQGAAESCSGGTDGITCGMNWAYGGWDGKYGLGEQMSALDCVMSMITVDHDPLTLLTGGSSEDSDVSAGNDTLSLSLYNTNLISISSGDKAGAAILTVVVLGSFLGGLVWMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ12</accession>
    <name>A0A1A0HJ12_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome biogenesis protein SLX9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_76774</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in ribosome biogenesis. Required for normal pre-rRNA processing in internal transcribed spacer 1 (ITS1). May be involved in the movements of the replication forks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with the 35S, 23S and 20S pre-rRNAs and with the U3 snoRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLX9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714309.1">
        <property type="nucleotide sequence ID" value="XM_018858584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ12"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ12"/>
    <dbReference type="GeneID" id="30031560"/>
    <dbReference type="OrthoDB" id="4068648at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028160">
        <property type="entry name" value="Slx9-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15341">
        <property type="entry name" value="SLX9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20360" checksum="DF0DCB020A6BD11D" modified="2016-10-05" version="1">MAGDFVKKSGLRDKASKNSALASKIEQFRRDQEPSQEAHDENPYLKLLKVSKKEKQQAKTNSFNERLASKVTFNLSGGVSKSALRRRKRKEKEQLKPKMDDLLSSLPEATVNIVDSSVAKKKAFVKGVSVKPSNLPNPQKHSGHTKLLEVENQRFSQVLQDKQFQQSPFAALRQAISQSKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ91</accession>
    <name>A0A1A0HJ91_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein L14b/L23e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_65987</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714705.1">
        <property type="nucleotide sequence ID" value="XM_018858358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ91"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ91"/>
    <dbReference type="GeneID" id="30031334"/>
    <dbReference type="OrthoDB" id="407959at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000003">
        <property type="entry name" value="60S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="137" mass="14432" checksum="8D1FEC854712956B" modified="2016-10-05" version="1">MSGSGASGNKFRMSLGLPVGAVVNCCDNSGARNLYILAVKGVGARLNRLPAASAGDMVMATVKKGKPELRKKVMPAIVVRQSKPWRRKDGTFLYFEDNAGVIVNPKGEMKGSAITGPVAKECADLWPRIASNSGVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD5</accession>
    <name>A0A1A0HJD5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histone-fold-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_35032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714750.1">
        <property type="nucleotide sequence ID" value="XM_018856559.1"/>
    </dbReference>
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    <dbReference type="STRING" id="869754.A0A1A0HJD5"/>
    <dbReference type="GeneID" id="30029535"/>
    <dbReference type="OrthoDB" id="653904at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017055">
        <property type="term" value="P:negative regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF5">
        <property type="entry name" value="DR1-ASSOCIATED COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="4">
        <location>
            <begin position="54"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="138" mass="15021" checksum="A5250B1BF82F3D7B" modified="2016-10-05" version="1">MADINSLINPPEETKESLDYKDESEEANEPAHAAADPAVSQDTILSSFDRIKTHFPAARIKTIMQSDEDIGKVAQATPVVVGRALEIFMANLVEAAILETKGAGVRRIAASHVRAAVEKTEQFDFLVDVVLKYPATKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJN3</accession>
    <name>A0A1A0HJN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TBC-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_10522</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018853790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJN3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJN3"/>
    <dbReference type="GeneID" id="30026766"/>
    <dbReference type="OrthoDB" id="17687at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TBC1 domain family member 9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="3">
        <location>
            <begin position="237"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="591"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="829"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="894"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="861"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="903"/>
            <end position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="924" mass="106426" checksum="C5486B337DEDBEE0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJR9</accession>
    <name>A0A1A0HJR9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714900.1">
        <property type="nucleotide sequence ID" value="XM_018857020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJR9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJR9"/>
    <dbReference type="GeneID" id="30029996"/>
    <dbReference type="OrthoDB" id="19159at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990334">
        <property type="term" value="C:Bfa1-Bub2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034586">
        <property type="entry name" value="Bfa1/Byr4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35140:SF1">
        <property type="entry name" value="MITOTIC CHECK POINT PROTEIN BFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="583" mass="67341" checksum="6DB840B6B1506CE7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJZ1</accession>
    <name>A0A1A0HJZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi to ER traffic protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Guided entry of tail-anchored proteins 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GET1</name>
        <name evidence="12" type="ORF">METBIDRAFT_36510</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Together with GET2, acts as a membrane receptor for soluble GET3, which recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. The GET complex cooperates with the HDEL receptor ERD2 to mediate the ATP-dependent retrieval of resident ER proteins that contain a C-terminal H-D-E-L retention signal from the Golgi to the ER.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the Golgi to ER traffic (GET) complex, which is composed of GET1, GET2 and GET3. Within the complex, GET1 and GET2 form a heterotetramer which is stabilized by phosphatidylinositol binding and which binds to the GET3 homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the WRB/GET1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714609.1">
        <property type="nucleotide sequence ID" value="XM_018856747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJZ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJZ1"/>
    <dbReference type="GeneID" id="30029723"/>
    <dbReference type="OrthoDB" id="69461at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03113">
        <property type="entry name" value="Get1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028945">
        <property type="entry name" value="Get1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027538">
        <property type="entry name" value="Get1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650:SF1">
        <property type="entry name" value="GUIDED ENTRY OF TAIL-ANCHORED PROTEINS FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42650">
        <property type="entry name" value="TAIL-ANCHORED PROTEIN INSERTION RECEPTOR WRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04420">
        <property type="entry name" value="CHD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Lumenal" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="181"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="218" mass="24446" checksum="BC1B0AEF97743726" modified="2016-10-05" version="1">MSFLDLLPTTILAFIFTVLLAKHVVSAIGKSTLVENAWSIYTWLASRMGHPKFKLLAAKRQELVKVNRERKAISAQDQYARWTKLNRTFDKLSGEISALTEEVSAEKASVGKVVNLTITVFTTAPIWFSRYWYRKVVLLYFPAGVFPYPVEWFLALPFTVTGGLGLTVWMFAVNSVLSSLTFLVKYALEPAVEKPELVSGEGKNNGQKTTDTEETAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK32</accession>
    <name>A0A1A0HK32_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TATA-box binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_36987</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715020.1">
        <property type="nucleotide sequence ID" value="XM_018856809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK32"/>
    <dbReference type="STRING" id="869754.A0A1A0HK32"/>
    <dbReference type="GeneID" id="30029785"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000120">
        <property type="term" value="C:RNA polymerase I transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008301">
        <property type="term" value="F:DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001092">
        <property type="term" value="F:TFIIA-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001188">
        <property type="term" value="P:RNA polymerase I preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="237" mass="25942" checksum="D91258514D267A12" modified="2016-10-05" version="1">MDSLKLPTTAASASTFTAPGSLSFPQGIDSAVTHVKKSEDGDIGVMEPNENDEDSAMSGIVPTLQNIVATVNLGCRLDLKTIALHARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVVTGAKSEDDSKLASRKYARIIQKLGFNAKFTDFKIQNIVGSCDVKFPIRLEGLAFSHGTFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKQREEIYAAFEAIYPVLSEFRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK70</accession>
    <name>A0A1A0HK70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_20925</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Lipid-anchor</topology>
            <topology evidence="10">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714901.1">
        <property type="nucleotide sequence ID" value="XM_018855023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK70"/>
    <dbReference type="STRING" id="869754.A0A1A0HK70"/>
    <dbReference type="GeneID" id="30027999"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000038">
        <property type="entry name" value="1,3-beta-glucanosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012946">
        <property type="entry name" value="X8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF2">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07983">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00768">
        <property type="entry name" value="X8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="X8" evidence="12">
        <location>
            <begin position="355"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="455"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="455"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="505" mass="53983" checksum="C1679CFD034B2E9F" modified="2016-10-05" version="1" fragment="single">ATDLPAIEISGNKFYYSNNGSQFLMRGVAYQQDTDSTSTTSFVDPLTDYDSCLRDIPYLQELQTNVIRVYALNVSQDHTDCMEALAAANIYVIADLSEPDLSIDRDDPTWTLELLDRYTSVVDAFHNYTNILGFFAGNEVTNNASNTDASAYVKAAVRDTKAYILEMGYKSIPVGYSSNDDSDIRLDLAEYFACGDESDRADFFGINMYEWCGDSTYETSGYETITEEYKNLGVPIFFSEYGCNEVSPRNFTEVQAIYSDEMTDVWSGGIVYMYYEETNNYGLVTIDNSEVSTLDDFNNLKSQIALIDPSYTTIGSVTTSATVTTCPTVGGYWSATTVLPPTPDSEVCDCMVASLSCVGDSTLDSDGIAELFSYVCGEVDCSGVTGNGTTGIYGSYSACKAKAQLDFVLNLYYSANGESSTACDFSGSASLQTASTASSCTAILSSAGEYGTGTVSGTVRTTTDSASTTTVSGSDSGSTSDSSSSSTSSSSTSGSASAHIAFSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK92</accession>
    <name>A0A1A0HK92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FMP52 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714921.1">
        <property type="nucleotide sequence ID" value="XM_018858662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK92"/>
    <dbReference type="STRING" id="869754.A0A1A0HK92"/>
    <dbReference type="GeneID" id="30031638"/>
    <dbReference type="OrthoDB" id="430436at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014843">
        <property type="entry name" value="Him1/Fmp52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14097:SF7">
        <property type="entry name" value="OXIDOREDUCTASE HTATIP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08732">
        <property type="entry name" value="HIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="226" mass="24185" checksum="0D29549C2810D897" modified="2016-10-05" version="1">MSCMIFGTTGLCGKAFLETAEKSSCFSAITSITRRPINSTSQKIKEIVEEDTDKYVNIINDEKPSIVFSGLATTRGAAGSAKAFVDIDHGINLKIAQASKDAGVNTFIIVSSVGASALSPFLYFKTKGRLEDDIIALKFPRTIILRPGPLIGQREASKGFGNDLSSAVFKHIHGTFLGRNIFYPIKASEVASAAVSLIQTPPQESSEPQVMIVGGRELLDVALSQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKG9</accession>
    <name>A0A1A0HKG9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_77127</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714784.1">
        <property type="nucleotide sequence ID" value="XM_018858646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKG9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKG9"/>
    <dbReference type="GeneID" id="30031622"/>
    <dbReference type="OrthoDB" id="2649at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042307">
        <property type="term" value="P:positive regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050684">
        <property type="term" value="P:regulation of mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14136">
        <property type="entry name" value="STKc_SRPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000409">
        <property type="entry name" value="CMGC/SRPK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000076">
        <property type="entry name" value="CMGC/SRPK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051334">
        <property type="entry name" value="SRPK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47634:SF9">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="135"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="79"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="635"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="655"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="665" mass="74410" checksum="56A3C6C1BD653F28" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKL3</accession>
    <name>A0A1A0HKL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">ATP-dependent RNA helicase DBP3</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP-dependent RNA helicase dbp3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_77311</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715012.1">
        <property type="nucleotide sequence ID" value="XM_018858675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKL3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKL3"/>
    <dbReference type="GeneID" id="30031651"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000464">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="147"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="348"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="22"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA24531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="528" mass="59332" checksum="7D80DDCC9F1BBFE5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKN9</accession>
    <name>A0A1A0HKN9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-rRNA-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77246</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the IPI1/TEX10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714938.1">
        <property type="nucleotide sequence ID" value="XM_018858665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKN9"/>
    <dbReference type="STRING" id="869754.A0A1A0HKN9"/>
    <dbReference type="GeneID" id="30031641"/>
    <dbReference type="OrthoDB" id="361362at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024679">
        <property type="entry name" value="Ipi1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056">
        <property type="entry name" value="REGULATOR OF MICROTUBULE DYNAMICS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16056:SF2">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12333">
        <property type="entry name" value="Ipi1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein Ipi1 N-terminal" evidence="8">
        <location>
            <begin position="137"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="366" mass="40448" checksum="8B5BCD109D48A04F" modified="2016-10-05" version="1">MGSKRKRNEKKKDFQKTKLKVGKTLAKPDNYTDTSFTAKSISLPNQTISKKSGGNGDVSHSKGDVDITHQLLLMKHHLASTRKEVVNFVEAHLPSNTSMYKQILTMTISLVTDPSASVRDAFVSLLSACADKQAGFLELHMRTIVLFVHSAMTHIKSEVRGSSTKVLDVLVKKAPIPLVKGHFVKTLRSYFPLMAWTLSGDKKAISLAVNTSDTLGGISSKVRTNHLAVLERLLESSLFEPEINASSFSIDWTKCTAVHPQSSRFLLPSNPQAYTQLKLYVDDLPTNLDVKSTEAAEGSFALSDIDSVSTEDQETRRKITYDVFLLPLRKNLGNAVKEGGEFGKQANKCLGVLERFEKAYEASQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HL09</accession>
    <name>A0A1A0HL09_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="11">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_77278</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714974.1">
        <property type="nucleotide sequence ID" value="XM_018858671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HL09"/>
    <dbReference type="STRING" id="869754.A0A1A0HL09"/>
    <dbReference type="GeneID" id="30031647"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.870:FF:000005">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000037">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00978">
        <property type="entry name" value="FAD_G3PDH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="12">
        <location>
            <begin position="69"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glycerophosphate oxidase C-terminal" evidence="13">
        <location>
            <begin position="472"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="648" mass="71848" checksum="B5AF1B33B366B5C3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ33</accession>
    <name>A0A1A0GZ33_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Prefoldin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_48148</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000012">
        <property type="protein sequence ID" value="OBA16950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709252.1">
        <property type="nucleotide sequence ID" value="XM_018858268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ33"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ33"/>
    <dbReference type="GeneID" id="30031244"/>
    <dbReference type="OrthoDB" id="248120at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23161">
        <property type="entry name" value="Prefoldin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000003">
        <property type="entry name" value="Prefoldin subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21431:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA16950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="116" mass="13409" checksum="E762AAD39DEF1006" modified="2016-10-05" version="1">MSDAKQQFEALSMEFTKAQSAMNELLEARSKLETQYQENKIVLEEFEAMDDGSKIYKLTGPVLMPQDYHEAKMNVSKRIEFIQGEISRVEEKVEKAQKDMEVSRTKLLQVRSQMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ59</accession>
    <name>A0A1A0GZ59_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NADH-ubiquinone oxidoreductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_80181</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I NDUFA5 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709310.1">
        <property type="nucleotide sequence ID" value="XM_018859187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ59"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ59"/>
    <dbReference type="GeneID" id="30032163"/>
    <dbReference type="OrthoDB" id="286811at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006806">
        <property type="entry name" value="NDUFA5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12653">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 13 KD-B SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04716">
        <property type="entry name" value="ETC_C1_NDUFA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA17013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15521" checksum="42A65FD21DF54476" modified="2016-10-05" version="1">MRFTRALRQVQEVLVKPASGYPIGLTGYYQHPNPRPALISLYNLTLQVLEKKFPKESVYRQSVEQMTKNRLQIVENNEISEQIENQIGGGLIEEIVVQAHDELTLANELGLLKAWEELEHKPLDDQWVYFGKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1R1</accession>
    <name>A0A1A0H1R1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphoribosylformylglycinamidine synthase</fullName>
            <ecNumber evidence="4">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Formylglycinamide ribonucleotide amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Formylglycinamide ribotide amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_33732</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709361.1">
        <property type="nucleotide sequence ID" value="XM_018856388.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H1R1"/>
    <dbReference type="GeneID" id="30029364"/>
    <dbReference type="OrthoDB" id="6666987at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.750:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000005">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000008">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000002">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.750">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00419">
        <property type="entry name" value="PurL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040707">
        <property type="entry name" value="FGAR-AT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055181">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010073">
        <property type="entry name" value="PurL_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01735">
        <property type="entry name" value="FGAM_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003672">
        <property type="entry name" value="PRK05297.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10099:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18076">
        <property type="entry name" value="FGAR-AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22689">
        <property type="entry name" value="FGAR-AT_PurM_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109736">
        <property type="entry name" value="FGAM synthase PurL, linker domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase N-terminal" evidence="20">
        <location>
            <begin position="43"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="19">
        <location>
            <begin position="210"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="18">
        <location>
            <begin position="474"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="FGAR-AT PurM N-terminal-like" evidence="21">
        <location>
            <begin position="691"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="18">
        <location>
            <begin position="869"/>
            <end position="1011"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00071729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF18076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF22689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA17966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1349" mass="146445" checksum="498A6FDCBED92F27" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1V1</accession>
    <name>A0A1A0H1V1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">LETM1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_33764</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709401.1">
        <property type="nucleotide sequence ID" value="XM_018856395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1V1"/>
    <dbReference type="STRING" id="869754.A0A1A0H1V1"/>
    <dbReference type="GeneID" id="30029371"/>
    <dbReference type="OrthoDB" id="275278at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097177">
        <property type="term" value="F:mitochondrial ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070131">
        <property type="term" value="P:positive regulation of mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033122">
        <property type="entry name" value="LETM1-like_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044202">
        <property type="entry name" value="LETM1/MDM38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009:SF1">
        <property type="entry name" value="MITOCHONDRIAL PROTON_CALCIUM EXCHANGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07766">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51758">
        <property type="entry name" value="LETM1_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Letm1 RBD" evidence="9">
        <location>
            <begin position="197"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="440"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="455"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA18006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="480" mass="54542" checksum="E3BB5F40E6172C70" modified="2016-10-05" version="1">MVSRSLCRSLPFNRQLALGRTLRPNYFPTPAAVFSVGPRKSNYFARYNSTKAGSNSPVEDLSKKVAPLQTVKHTELSQDVAAQELVQSGQKDDAMPAAPKKTIWEKVKHEVAHYWAGTKLLGYEIKISTKLLFKLASGYGLSRRESNQLQRTIVDVARLIPFSMFLIVPFAELLLPVALKIFPNLLPSTYESSSDVKKKRARLSKARANASEFIKKTMEESGLKLSKKISEKEREEFVSFFHTISMGKTPAKEHLIKVARMFKNDQVLDNLSRHQLMAMTKYMSLRPFGTDSILRYQIRHRLLTIIKDDKAIDYEGVDSLTVPELQLACSQRGIKTQDASPARLREDLDTWLDLRLRQKIPSTLLILSSAYTYGEHTQGVDTYYSALLAVLSSIPDEVYNVAKLELADDSKLKLDILKEQDEMINEENIREKDTVNQIKDNIKLDEYEETATEGMKIEQDDDKSSEQSKESAEQKPEPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H214</accession>
    <name>A0A1A0H214_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sedlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_226573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709393.1">
        <property type="nucleotide sequence ID" value="XM_018855183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H214"/>
    <dbReference type="STRING" id="869754.A0A1A0H214"/>
    <dbReference type="GeneID" id="30028159"/>
    <dbReference type="OrthoDB" id="10252102at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14825">
        <property type="entry name" value="TRAPPC2_sedlin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA17998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="159" mass="17832" checksum="96115E7921243A93" modified="2016-10-05" version="1">MASYYFTIIGTRDNPLYELEFSSFKSGHSSIPGEARFSPKVKELLPFISNASLDIVEDAQWQTSGFNLGKVDSFYGVLVNAFITQGNIKLVLCSDASTSSESTISMTSTKQDETAIRHFFTEAYELYVKAQLNPFYAVNDPLVSPDFDHRVKSLARKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H281</accession>
    <name>A0A1A0H281_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Family A G protein-coupled receptor-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_19911</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709455.1">
        <property type="nucleotide sequence ID" value="XM_018854909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H281"/>
    <dbReference type="STRING" id="869754.A0A1A0H281"/>
    <dbReference type="GeneID" id="30027885"/>
    <dbReference type="OrthoDB" id="5368598at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023041">
        <property type="entry name" value="Glucose_rcpt_Git3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022596">
        <property type="entry name" value="GPR1/2/3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11710">
        <property type="entry name" value="Git3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11970">
        <property type="entry name" value="GPR_Gpa2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="328"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose receptor Git3-like N-terminal" evidence="7">
        <location>
            <begin position="12"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="G protein-coupled receptor GPR1/2/3 C-terminal" evidence="8">
        <location>
            <begin position="315"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="262"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="268"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="281"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF11970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA18060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="514" mass="59395" checksum="26E4C1A2636685E4" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4N1</accession>
    <name>A0A1A0H4N1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Heat shock transcription factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Heat shock factor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_33655</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HSF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709569.1">
        <property type="nucleotide sequence ID" value="XM_018856368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4N1"/>
    <dbReference type="STRING" id="869754.A0A1A0H4N1"/>
    <dbReference type="GeneID" id="30029344"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00434">
        <property type="entry name" value="HSF_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="11">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="528"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00084017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="606" mass="69066" checksum="B37CEB738DBEA769" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4W6</accession>
    <name>A0A1A0H4W6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709503.1">
        <property type="nucleotide sequence ID" value="XM_018858176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4W6"/>
    <dbReference type="GeneID" id="30031152"/>
    <dbReference type="OrthoDB" id="20473at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="199" mass="22641" checksum="5173956485C59023" modified="2016-10-05" version="1">MEDITNRQKKAKLDSKPFYPPTQERLPSPSSPSTSLSPQRPHQRQKSVLFELSRNEHFECPLLTPRDEDYDLDSDHIEEKSQEISLGALDSNIDYVALGSTASLLDLTKEHIETEITELASLRTQAQKASKLDLVDFYIRLIYDKQPLPAQHTIVRAPTIDWAKYEEGLANVSLHHDCTNDEENPVFQTMNMFGSASKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H558</accession>
    <name>A0A1A0H558_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histidine--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_33420</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the aminoacylation of histidyl-tRNA in both the cytoplasm and the mitochondrion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709746.1">
        <property type="nucleotide sequence ID" value="XM_018856291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H558"/>
    <dbReference type="STRING" id="869754.A0A1A0H558"/>
    <dbReference type="GeneID" id="30029267"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000015">
        <property type="entry name" value="Histidyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000021">
        <property type="entry name" value="Probable histidine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core" evidence="17">
        <location>
            <begin position="44"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon-binding" evidence="16">
        <location>
            <begin position="413"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="314"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00058343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF13393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA19214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="532" mass="58584" checksum="17625FA801520DDB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5E2</accession>
    <name>A0A1A0H5E2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_13602</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709836.1">
        <property type="nucleotide sequence ID" value="XM_018854397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5E2"/>
    <dbReference type="STRING" id="869754.A0A1A0H5E2"/>
    <dbReference type="GeneID" id="30027373"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000007">
        <property type="entry name" value="Nitrogen regulatory AreA, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="210"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="100"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="353"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="45"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="61"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="117"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="179"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="636" mass="68152" checksum="D14E92F335A6A77F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Q9</accession>
    <name>A0A1A0H5Q9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homocysteine S-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33444</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709774.1">
        <property type="nucleotide sequence ID" value="XM_018856298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Q9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Q9"/>
    <dbReference type="GeneID" id="30029274"/>
    <dbReference type="OrthoDB" id="261426at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008898">
        <property type="term" value="F:S-adenosylmethionine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033528">
        <property type="term" value="P:S-methylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051486">
        <property type="entry name" value="Hcy_S-methyltransferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007020">
        <property type="entry name" value="PRK09485.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015:SF1">
        <property type="entry name" value="HOMOCYSTEINE S-METHYLTRANSFERASE-LIKE ISOFORM 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46015">
        <property type="entry name" value="ZGC:172121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="330" mass="36568" checksum="BB935401CAE44BC0" modified="2016-10-05" version="1">MSLKQVLAEKILIQDGSMGTKLENILPLDHPLSVKGSPLWSTKLLLGEPSLITQVHKSYVDAGCDMLITSTYQASMLTLEKHANMSIEDVHRTWQRSVDCAKEAINTSNRTDKIYIAGSIGPYGAFLANGAEYSGDYNGMSSSELANYHSELVKYFVENPDVDVIAFETIPNFDEVKGVFQLVKSLYSNTYGKQFYLNFSCKDQFTLADGTPLSEVVDYMVSEMAGHVEEYFVGTGCNCVAYEIVKGVAETVNKACEKRNVKPLNLIVYPNLGFNNDMSDPSKYGFLSDRDGWRKAVQEWLTVPNIRVIGGCCSTSPSEIAVIHEVINNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H667</accession>
    <name>A0A1A0H667_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Outer kinetochore protein DAD4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_45952</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710049.1">
        <property type="nucleotide sequence ID" value="XM_018857974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H667"/>
    <dbReference type="STRING" id="869754.A0A1A0H667"/>
    <dbReference type="GeneID" id="30030950"/>
    <dbReference type="OrthoDB" id="5516652at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013959">
        <property type="entry name" value="DASH_Dad4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28222">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28222:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08650">
        <property type="entry name" value="DASH_Dad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="15" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA19521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="72" mass="8370" checksum="54E3BAC283A35DEB" modified="2016-10-05" version="1">MNNPHEEVQLALITRIVNNMKSLNESVSDMNLTLNEINNKNKDVEALTRMWHNYAKSTEYHLETTGQTRDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H674</accession>
    <name>A0A1A0H674_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AHC1-like C2H2 zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_45871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709988.1">
        <property type="nucleotide sequence ID" value="XM_018857968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H674"/>
    <dbReference type="STRING" id="869754.A0A1A0H674"/>
    <dbReference type="GeneID" id="30030944"/>
    <dbReference type="OrthoDB" id="5355528at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058706">
        <property type="entry name" value="zf-C2H2_AHC1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25909">
        <property type="entry name" value="zf-C2H2_AHC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHC1-like C2H2 zinc-finger" evidence="2">
        <location>
            <begin position="144"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="445" mass="50113" checksum="DC7A6178731B2A4A" modified="2016-10-05" version="1">MVEKDDLIKKLENVPEEVLRSILTNQVDLEIRLKHKELKLTEEEIGKCEAQMLALRKFFEVPNEVSFRSEPNDFTLKYFDILNKSLSVNYTKLQQKQYALENPVFNEAMFTESEPTTHTYRTRSTTSSLRPSIGGPAVRIAGCLYRRTDGVVVKLTCTGCQRSNFSSAQGFLNHNRIAHSREYASQDSAALECGEVLPDEFQDEEGLASLVSLRQLDVNPAKNLKTREGYFDGLNTPTNKTGALSYSNSYPEHSSKPATRKSTPESSKEPKAQAKVAKDVLRDSLMEGVGTQELMKKLISSGLARDAKDFQSLIGEVQKEVSNSHLFADEEEVVEEVEGQDKREEKNSLQETNGPEKPKRGASETQRPKIQKNNDPLLQAGDSTVKPETTDTSVLVRAEIGGKYENRLRRRKSRAEMSLAGTETEDEPRAKGSEDRRHKRKKSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6D7</accession>
    <name>A0A1A0H6D7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_73653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710053.1">
        <property type="nucleotide sequence ID" value="XM_018858418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6D7"/>
    <dbReference type="GeneID" id="30031394"/>
    <dbReference type="OrthoDB" id="2020070at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="537" mass="59435" checksum="C6D58C20E4A628FA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6M3</accession>
    <name>A0A1A0H6M3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_206969</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710264.1">
        <property type="nucleotide sequence ID" value="XM_018855003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6M3"/>
    <dbReference type="GeneID" id="30027979"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="195" mass="20933" checksum="8CD2160965531934" modified="2016-10-05" version="1">MRTLETEVGTGSMPAADEHGLTADEHGLVEPRLQVRPDLTPLLLGGRGKGNLPELGYEIRAYFAAASGPGPAGKLGPAVGLDFPGQPLIFRERKKKDLQSLGLVQVPPCPVRLQYLPGLAGPCSLCRDNPPCAAVPFFCQACRHLRTTRGLVSGPWNRSPNLWRKCWHLVFLAIRIFLGVAPFFAGGGHPLLLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Q9</accession>
    <name>A0A1A0H6Q9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein arginine methyltransferase NDUFAF7</fullName>
            <ecNumber evidence="7">2.1.1.320</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_79142</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Arginine methyltransferase involved in the assembly or stability of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-arginyl-[protein] + 2 S-adenosyl-L-methionine = N(omega),N(omega)'-dimethyl-L-arginyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:48108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:88221"/>
            <dbReference type="EC" id="2.1.1.320"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NDUFAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.320" evidence="7"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710304.1">
        <property type="nucleotide sequence ID" value="XM_018859008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Q9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Q9"/>
    <dbReference type="GeneID" id="30031984"/>
    <dbReference type="OrthoDB" id="5595109at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035243">
        <property type="term" value="F:protein-arginine omega-N symmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12710:FF:000008">
        <property type="entry name" value="Protein arginine methyltransferase NDUFAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049:SF7">
        <property type="entry name" value="PROTEIN ARGININE METHYLTRANSFERASE NDUFAF7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="324"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="539" mass="60194" checksum="16199562D0753416" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6R2</accession>
    <name>A0A1A0H6R2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartyl aminopeptidase</fullName>
            <ecNumber evidence="4">3.4.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_46317</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.</text>
            <dbReference type="EC" id="3.4.11.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.21" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710126.1">
        <property type="nucleotide sequence ID" value="XM_018858024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6R2"/>
    <dbReference type="STRING" id="869754.A0A1A0H6R2"/>
    <dbReference type="GeneID" id="30031000"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF3">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11 12" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA19598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="464" mass="51090" checksum="A4442AE73443B0D0" modified="2016-10-05" version="1">MDSETQTKAQGLVDFITYSPTPYHAVANVKQKLTLDGFVVLKEKDQWDLQPGQKYVVTRNASSLIAFKIGQSFRKGGPVAIVGAHTDSPCLRIKPISKKSGEGFIQIGVEQYGGLIAHSWFDRDLSVAGRVYVREKSGKYVPRLVHLRKPLMRIPTLAIHLDREVNKKFEFNKETELLPIAGQEKFGHSCADDPNLQLSTSEFEAVKHVVERHNESLLILIAKDLGVEVSQIEDFELLLCDYQDATLGGMHDEFIFAPRQDNLTSCFASMEALLASEPAEEGALMISLFDHEEIGSVSAQGADSSFLPDVLARLADSDNGAMARMCSRSFLLSSDQAHGVHPNYERVYEKANRPHVNGGPVIKINANQRYATNSRGIVLIKAVAQRAKVPLQLFVVRNDSPCGSTIGPMLSAKLGLRTLDLGNPQLSMHSIRETGGAYDVVRLTKLFGGFFAEYVDVDAAVECD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U7</accession>
    <name>A0A1A0H6U7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance of mitochondria protein 21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_208435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710344.1">
        <property type="nucleotide sequence ID" value="XM_018855012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U7"/>
    <dbReference type="GeneID" id="30027988"/>
    <dbReference type="OrthoDB" id="4096963at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="388"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="492"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="503"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="555"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="618"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="685"/>
            <end position="698"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="705" mass="75948" checksum="289FA69E9DE73F3D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H782</accession>
    <name>A0A1A0H782_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_79223</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. ADCK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710411.1">
        <property type="nucleotide sequence ID" value="XM_018859020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H782"/>
    <dbReference type="STRING" id="869754.A0A1A0H782"/>
    <dbReference type="GeneID" id="30031996"/>
    <dbReference type="OrthoDB" id="201153at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13970">
        <property type="entry name" value="ABC1_ADCK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004147">
        <property type="entry name" value="ABC1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034646">
        <property type="entry name" value="ADCK3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051409">
        <property type="entry name" value="Atypical_kinase_ADCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43851:SF3">
        <property type="entry name" value="COENZYME Q8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03109">
        <property type="entry name" value="ABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ABC1 atypical kinase-like" evidence="6">
        <location>
            <begin position="221"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="570" mass="64355" checksum="2A81E8C9E1B6DF18" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D6</accession>
    <name>A0A1A0H7D6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_79168</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710338.1">
        <property type="nucleotide sequence ID" value="XM_018859013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7D6"/>
    <dbReference type="GeneID" id="30031989"/>
    <dbReference type="OrthoDB" id="10252281at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="6">
        <location>
            <begin position="167"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="5">
        <location>
            <begin position="502"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="72"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="667" mass="75708" checksum="C4948C43A4843D44" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7P6</accession>
    <name>A0A1A0H7P6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="3 8">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_45537</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="3 8"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710530.1">
        <property type="nucleotide sequence ID" value="XM_018857929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7P6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7P6"/>
    <dbReference type="GeneID" id="30030905"/>
    <dbReference type="OrthoDB" id="10039976at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004059">
        <property type="term" value="F:aralkylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000136">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16730" checksum="D7BD6B89C81CC6CB" modified="2016-10-05" version="1">MALPQGYKVRAVQANDHKQYMATLSVLTSVGDVTEMKFLELVRHWDKYSEIYYPKVIVDSNDCVVATGMLFIEQKLIHGCGRVGHIEDIAVAKSQQGLKVGNMLIQHLSKIAEEAGCYKVILDCSKSNVGFYEKCGYKDAGIEMCKRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7V4</accession>
    <name>A0A1A0H7V4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Replication protein A, subunit RPA32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33039</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710590.1">
        <property type="nucleotide sequence ID" value="XM_018856200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7V4"/>
    <dbReference type="STRING" id="869754.A0A1A0H7V4"/>
    <dbReference type="GeneID" id="30029176"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030491">
        <property type="term" value="P:heteroduplex formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04478">
        <property type="entry name" value="RPA2_DBD_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014646">
        <property type="entry name" value="Rfa2/RPA32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014892">
        <property type="entry name" value="RPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08784">
        <property type="entry name" value="RPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036949">
        <property type="entry name" value="RPA32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="6">
        <location>
            <begin position="69"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A C-terminal" evidence="7">
        <location>
            <begin position="188"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="258" mass="28490" checksum="EF901D6E26E8B78B" modified="2016-10-05" version="1">MDYANYGNYGDGGFNTDAQFTDMNGGEGSQRQLQTRNTLSPVTIKQINDATQPVQDGEFTINKVSLNMVSFVGVVRKVENQTSAVTVTIEDGTGSVDVKKWVDEKLTTAAEEEEYFLGLENNYVYVTGALKEFNLKKAIQHAQIRPITDHNEVIYHMFYTIHTHLEAEGLLNVPKQESSSGLFVGSSTGAGEDSKLSDQDRIMSVIAANTGSMLEGVPVKWISDSLNIPENEVRNTCQYLSEQGKVYQGYDESSFLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H842</accession>
    <name>A0A1A0H842_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_213516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710680.1">
        <property type="nucleotide sequence ID" value="XM_018855061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H842"/>
    <dbReference type="STRING" id="869754.A0A1A0H842"/>
    <dbReference type="GeneID" id="30028037"/>
    <dbReference type="OrthoDB" id="5982228at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000102">
        <property type="term" value="F:L-methionine secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042883">
        <property type="term" value="P:cysteine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903692">
        <property type="term" value="P:methionine import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000025">
        <property type="entry name" value="High-affinity methionine permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF498">
        <property type="entry name" value="HIGH-AFFINITY METHIONINE PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="227"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="540" mass="58995" checksum="3A0E58B6F7751791" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H847</accession>
    <name>A0A1A0H847_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_213583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710685.1">
        <property type="nucleotide sequence ID" value="XM_018855063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H847"/>
    <dbReference type="GeneID" id="30028039"/>
    <dbReference type="OrthoDB" id="4074735at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071986">
        <property type="term" value="C:Ragulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020233">
        <property type="entry name" value="Slm4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16818">
        <property type="entry name" value="SLM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="16024" checksum="37DD35442B31AD16" modified="2016-10-05" version="1">MLQQREISKLLAQAVTHAGSLEHAPDAVLFVSLLSARGLPLITVGSPDAESCISPEALRMYSLMTTNLFKQQPKTGDASLDHWAVLDVDTSLRAVIRKFATTSSGTNEPPTTFYTVLFYSSAYADTQAKVRLDLVTAALTAGLSGYRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8H4</accession>
    <name>A0A1A0H8H4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_195810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710812.1">
        <property type="nucleotide sequence ID" value="XM_018854871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8H4"/>
    <dbReference type="GeneID" id="30027847"/>
    <dbReference type="OrthoDB" id="4074976at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="624" mass="70710" checksum="A0655867B846DC6A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8P3</accession>
    <name>A0A1A0H8P3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BRO1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the palA/RIM20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710887.1">
        <property type="nucleotide sequence ID" value="XM_018857679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8P3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8P3"/>
    <dbReference type="GeneID" id="30030655"/>
    <dbReference type="OrthoDB" id="64867at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08915">
        <property type="entry name" value="V_Alix_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.560">
        <property type="entry name" value="alix/aip1 in complex with the ypdl late domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.50">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.280">
        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025304">
        <property type="entry name" value="ALIX_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030">
        <property type="entry name" value="PCD6 INTERACTING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23030:SF39">
        <property type="entry name" value="PROGRAMMED CELL DEATH 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13949">
        <property type="entry name" value="ALIX_LYPXL_bnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="3">
        <location>
            <begin position="4"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="629"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="765" mass="85700" checksum="44DB89E46C66075F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H964</accession>
    <name>A0A1A0H964_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">PQ-loop-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_44149</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the laat-1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711062.1">
        <property type="nucleotide sequence ID" value="XM_018857749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H964"/>
    <dbReference type="GeneID" id="30030725"/>
    <dbReference type="OrthoDB" id="8048523at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098588">
        <property type="term" value="C:bounding membrane of organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015174">
        <property type="term" value="F:basic amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034488">
        <property type="term" value="P:basic amino acid transmembrane export from vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034490">
        <property type="term" value="P:basic amino acid transmembrane import into vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000011">
        <property type="entry name" value="PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000012">
        <property type="entry name" value="Vacuolar membrane PQ loop repeat protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF35">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER YPQ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="73"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="248"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="301" mass="33733" checksum="AD891E8233D60645" modified="2016-10-05" version="1">MAPPPPVIIDAQVVSGITGSISLACWIIVFAPQIYENFRRKSSDGLSLMFIVLWLAGDVFNVLGAVLQGVLPTMIVLAVYYTLADVVLLLQCLMYGDGKQPDFVHLSPANPLNDDVLETALSRELIEVESEITVTNSNSRFSPVKAFLHKLFMVSLVFSAGLISWYISYSKNHGKQHKAPEEMEFDPLAQFFGWLCAILYLGSRIPQILLNYERKSCEGISFMFFLFACLGNLTYVISILAIDMSWNYIWVNLSWLVGSLGTLGLDFTIFIQFFLYNENGETESEEQEQLIGGLQLQYSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9A6</accession>
    <name>A0A1A0H9A6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">UPF0057-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_78833</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710980.1">
        <property type="nucleotide sequence ID" value="XM_018858952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9A6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9A6"/>
    <dbReference type="GeneID" id="30031928"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF57">
        <property type="entry name" value="PLASMA MEMBRANE PROTEOLIPID 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="83"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="145" mass="15895" checksum="225B3737F2A267A4" modified="2016-10-05" version="1">MCCCECMSDILLVVVSVLFPPLPVWIRRGLCSMDSLVNVLLCVLGYFPGLVHSWYIIAKYPPYSRRSGPKVYHFHQTSADLEGQRHHHHHHHHHCSPANSTVSLQPLASFSPTYGSTNDSSAQEGSSSGGPAGAPPAYSELPSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9B1</accession>
    <name>A0A1A0H9B1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glucose/xylose facilitator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32466</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710985.1">
        <property type="nucleotide sequence ID" value="XM_018856051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9B1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9B1"/>
    <dbReference type="GeneID" id="30029027"/>
    <dbReference type="OrthoDB" id="5141738at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17356">
        <property type="entry name" value="MFS_HXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000044">
        <property type="entry name" value="Hexose transporter Hxt3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF75">
        <property type="entry name" value="GALACTOSE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="183"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="470"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="506"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="534"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="107"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="609" mass="67719" checksum="187E9D804642AE95" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9D8</accession>
    <name>A0A1A0H9D8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78858</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711024.1">
        <property type="nucleotide sequence ID" value="XM_018858962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9D8"/>
    <dbReference type="STRING" id="869754.A0A1A0H9D8"/>
    <dbReference type="GeneID" id="30031938"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002236">
        <property type="term" value="P:detection of misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070843">
        <property type="term" value="P:misfolded protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035719">
        <property type="term" value="P:tRNA import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000002">
        <property type="entry name" value="Dnaj homolog subfamily b member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000013">
        <property type="entry name" value="DnaJ subfamily B member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051339">
        <property type="entry name" value="DnaJ_subfamily_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
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        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="336" mass="35882" checksum="2A6B7092622512F5" modified="2016-10-05" version="1">MVKETKLYDLLGVSPSASESEIKKAYRKTALKYHPDKPTGDTEKFKEISEAFDILSNGDKRQVYDDYGLEAARGNAPAGGNPFAGAGGAGGAGGMPGGTHFSFGGAGGNPFSQADAFNIFSQMGGFGMDDGFSRGGFGGGSGFGGGMPGGFGGGARSARMPEPDTVPMQLPVSLEDLYAGSTKKMKLNRKSADGTKESKILEVKIKPGWKAGTKINFANEGDYQPECHARQTVQFVIEERPHSVFKRDGNDVTATIPLTFKEALLGFDKEVMTLSGRKINISRSTPVQPSSTNRYPNLGMPISKEPGRFGDLIVKYKIDFPVHLSADQKEAIKRAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9G6</accession>
    <name>A0A1A0H9G6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32511</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711040.1">
        <property type="nucleotide sequence ID" value="XM_018856065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9G6"/>
    <dbReference type="GeneID" id="30029041"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="10058" checksum="9AD31D7978115A29" modified="2016-10-05" version="1">MTQPNIIFAHTKSETMDTSQTLSLAMCIRNVKFPNTFWQELRFDSVRQLFVKLNKLKLVHHHNLQCIQSRMWQVAGKAFLESAPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9G7</accession>
    <name>A0A1A0H9G7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">TMS membrane protein/tumor differentially expressed protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78961</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDE1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711157.1">
        <property type="nucleotide sequence ID" value="XM_018858978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9G7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9G7"/>
    <dbReference type="GeneID" id="30031954"/>
    <dbReference type="OrthoDB" id="5963193at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005016">
        <property type="entry name" value="TDE1/TMS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383">
        <property type="entry name" value="SERINE INCORPORATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10383:SF9">
        <property type="entry name" value="SERINE INCORPORATOR, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03348">
        <property type="entry name" value="Serinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="443"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="476" mass="51791" checksum="FA1CCE3B5CCE9DC9" modified="2016-10-05" version="1">MGALVSIPLAGTSWLASCFGAAACSALCSLLGGSFQSATMTRLSYAVLLLVNSLVSWIALSPFVVHKLEKVLFGFINSRCGPDGKQCISFTSVHRINLALGVLHLVLAALLANVRSTANPRNVIQNGCWKWKVAAWAALVAVNFLAIPDAFFVFYGNNIAIVFSTVFLGIGLVLLVDFAHAWAEKCLEQIELEELTGGDEYNAAFWKKLLVGGTLSMYVCSVVLTVAMYVFFAGPGCSMNQAAISVNLVLAVLISAVSVNQSVQESNPHAGLAQALMVVFYCSYLVLSAVVSEPDDKLCNPLVRSRGTRTLSVFLGALFTFVALAYTTTRAANSSFFEPEHPPAVSAPISAQPSERNQMRYAAIKQAVDEGSLPESALSQLDLYEDESRYGAGPDDRQVKYNYTVFHIIFFLATQYISTLLTINVTQDNVGDFVPVGRTYFSSWVKIISSWVCYVLYGWSLVAPMLWPDRFSTMQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9K6</accession>
    <name>A0A1A0H9K6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Superoxide dismutase 1 copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_32766</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711331.1">
        <property type="nucleotide sequence ID" value="XM_018856127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9K6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9K6"/>
    <dbReference type="GeneID" id="30029103"/>
    <dbReference type="OrthoDB" id="666972at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="HMA, heavy metal-associated domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="4">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="164" mass="17474" checksum="5E3FFE7D848A8228" modified="2016-10-05" version="1" fragment="single">MTSDQTFETVFNVPMECQACVESVEAALRQLPLHGFKADLKTETVTAVGQVSPSAIVKAIQETGKDAIIRGTGKPNSAAVCILETFDESDREHPVKGLARIVAVSDQHIYVDLTVNGLAQGVYYPQIRALGNLSRGAESTGSLFHAFSAVEVNQPSSAESTVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9K9</accession>
    <name>A0A1A0H9K9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_32551</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711094.1">
        <property type="nucleotide sequence ID" value="XM_018856079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9K9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9K9"/>
    <dbReference type="GeneID" id="30029055"/>
    <dbReference type="OrthoDB" id="3938623at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050781">
        <property type="entry name" value="CWC22_splicing_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003891">
        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18034:SF3">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC22 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02847">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51366">
        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MI" evidence="8">
        <location>
            <begin position="292"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="506"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="512"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="554" mass="63080" checksum="E7838136A77E6713" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9N0</accession>
    <name>A0A1A0H9N0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_44111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711243.1">
        <property type="nucleotide sequence ID" value="XM_018857742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9N0"/>
    <dbReference type="GeneID" id="30030718"/>
    <dbReference type="OrthoDB" id="4088176at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="164" mass="17566" checksum="EE8365D1A0EEE9B1" modified="2016-10-05" version="1">MKNPFSSAPKKELPSDEPPPYEQLEAIHSPEKGDQKVPEAPLYSETYGPNEYPPEKGGPPVAPRPDWQNGNTSSPGHVPPGTYTYTVAPQTANIAYDIEGRNARPGYAQYLQTDAQRIAQGDAPKPREAFGRGGAPLAPSKKSLGQSGGFPGALGPTYYDSSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9N8</accession>
    <name>A0A1A0H9N8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U5 small nuclear ribonucleoprotein 200 kDa helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_43233</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711115.1">
        <property type="nucleotide sequence ID" value="XM_018857629.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H9N8"/>
    <dbReference type="GeneID" id="30030605"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000388">
        <property type="term" value="P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000133">
        <property type="entry name" value="Pre-mRNA splicing helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000013">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="485"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="699"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="1326"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="198"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="358"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1773"/>
            <end position="1800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1775"/>
            <end position="1796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2117" mass="234863" checksum="D6962442027BD416" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q4</accession>
    <name>A0A1A0H9Q4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative pentamidine resistance factor, mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_44241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711268.1">
        <property type="nucleotide sequence ID" value="XM_018857765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q4"/>
    <dbReference type="GeneID" id="30030741"/>
    <dbReference type="OrthoDB" id="73691at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="403" mass="44838" checksum="90E1D5515DF3DABB" modified="2016-10-05" version="1">MSSRGLFLYGTLRKQFLLALDRSPMLQSLWSQQTASAVNVPLTLPLSMPLKSQIYLDVALTNAGKPAVQVKAKQGLAYVKQLMRFYKHGVSAVWRNHKQAKTLMKQSYKMSGSLGRAGRETAVRVPGPRALTRHMAQHLYMNLVENRAEKAATTGDVVRAGAAAEACVSPGLLGMSRADFQLLRRTPPDFVKIPAFAVLCAIFMETTPVLCYAFPELTPLTCVLPLLLPRIWRPGPMKDLQAQMTPAEIGLVADYMMKTAYNMPPRHVRLLCAALRLKTKYVPSQMFPEFVLRKRLHDYFNYLTVDNYYLSGMNGDGNVWNLDVQELVLACLERNLVDDCKALVGVLSSGSLESRADALDALRLKLVRFIADFSSANVGYLAVGHLLESPDVKTVTKWRESGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q9</accession>
    <name>A0A1A0H9Q9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glucan 1,3-beta-glucosidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_78005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711374.1">
        <property type="nucleotide sequence ID" value="XM_018858801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Q9"/>
    <dbReference type="GeneID" id="30031777"/>
    <dbReference type="OrthoDB" id="1887033at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046557">
        <property type="term" value="F:glucan endo-1,6-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF43">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="470" mass="54303" checksum="E10FF0DCBDF32661" modified="2016-10-05" version="1">MYQTRSNFGANLGSVFLREKWIYPSFFKGEQKTEFDIISSFKKNKEKGRREMEAHWTSYFLDEDWVELTRNKVNSLRLPIGFWNIDGGKYTTGTIFEEYSEMYRNSWLIIKSHYIVPAARRGISILIDVHGVPGGANDNDHSGAFYGGATFWTTPSFQELFIKAAVFVFEDLKEYDNISGILLINEASYNFNLQQQNFYAAAINAIREKEKTIPVVISDGWNADSWIQWVSSNQNPNAPAGIVVDHHVYRTFTDKDKERSADEIIADLNHDLLPSVNNDGNDVDFMVGEWSSVMDGQTWARTNGVITPSDDNDPRRRELVAEFNSKQTELFRKRASTGSYFWTYKFESGYGGEWDFRQQLGRGFEAPQVSVPDDSTFKRTLARDYTAHKNYWDSQYPNGKFEHERYKEGFVTAWEDGVTYAKAGALVGRKHAIKSVRLQEHLQRKGYLENIWEWKQGFDKGLEEFNKNAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9R2</accession>
    <name>A0A1A0H9R2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711144.1">
        <property type="nucleotide sequence ID" value="XM_018854238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9R2"/>
    <dbReference type="STRING" id="869754.A0A1A0H9R2"/>
    <dbReference type="GeneID" id="30027214"/>
    <dbReference type="OrthoDB" id="21214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031511">
        <property type="term" value="C:Mis6-Sim4 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025207">
        <property type="entry name" value="Sim4_Fta4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13093">
        <property type="entry name" value="FTA4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="194"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="302" mass="34497" checksum="92668B3522459693" modified="2016-10-05" version="1">MSLVSSPVLVQMSYLQIKRFVDEQIKALNTPLILNDDIKRLLHQEGISEGQAQSILFKCNLLMKRHNRTKFNKQVVHQIVQQVAKAEQENLMQVNKALSKISRLAQPIMTDQFASSGRLEDRLRLLSNLADILPEGRYLFAVHGDNESESQPPDDAETQAPGSEDPNPLVRDDEELVQSPSELEMQNQYLKATKHELDQQIGAYNEKRDKLRQQYDDLRMQLAHLNEDIVYKMEKLAYLRSLNLKMDFLGARPDEKCVDSCDSDGDDEEEAVVENSEDEGLGPQMTRFSVLVEKLEFALRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W9</accession>
    <name>A0A1A0H9W9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ribonuclease H-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_21077</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oligoribonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711194.1">
        <property type="nucleotide sequence ID" value="XM_018855035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9W9"/>
    <dbReference type="GeneID" id="30028011"/>
    <dbReference type="OrthoDB" id="270189at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06135">
        <property type="entry name" value="Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000003">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003765">
        <property type="entry name" value="PRK05359.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046:SF0">
        <property type="entry name" value="OLIGORIBONUCLEASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="5">
        <location>
            <begin position="19"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="22813" checksum="49934E01F876978C" modified="2016-10-05" version="1" fragment="single">PLQSVKPTKRKYSGSIFKPLVWIDCEMTGLDISKDHIIEICCIITDGNLNIVDEKGYESTVYVPKKVLDNMNEWCVTHHGQSGLTAKVLANPLQTLAKVEQELLEYIQTYISEPRTSLLAGNSVHMDKFFMMKEFPRVIDHLHYRLVDVSSIMEVGYRHNPQLMKMYPKKKGAHTARSDILESIEQLRWYTHNYLRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA84</accession>
    <name>A0A1A0HA84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_205825</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LU7TM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711309.1">
        <property type="nucleotide sequence ID" value="XM_018854983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA84"/>
    <dbReference type="STRING" id="869754.A0A1A0HA84"/>
    <dbReference type="GeneID" id="30027959"/>
    <dbReference type="OrthoDB" id="19932at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053937">
        <property type="entry name" value="GOST_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009637">
        <property type="entry name" value="GPR107/GPR108-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053938">
        <property type="entry name" value="PTM1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229:SF1">
        <property type="entry name" value="GH17801P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21229">
        <property type="entry name" value="LUNG SEVEN TRANSMEMBRANE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06814">
        <property type="entry name" value="GOST_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21902">
        <property type="entry name" value="PTM1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291755" evidence="9">
        <location>
            <begin position="17"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="384"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="429"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="PTM1-like N-terminal" evidence="11">
        <location>
            <begin position="26"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="GOST seven transmembrane" evidence="10">
        <location>
            <begin position="183"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="493"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="549"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF06814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="567" mass="64003" checksum="68C1A77517DA5DC0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAA9</accession>
    <name>A0A1A0HAA9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_206069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711334.1">
        <property type="nucleotide sequence ID" value="XM_018854990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAA9"/>
    <dbReference type="GeneID" id="30027966"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508816" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="84" mass="8989" checksum="28D96202E23CAE93" modified="2016-10-05" version="1">MSVFVFLVVFSFVCVLGRVLCGVVSFGSSGQYFVLPCILFSLFSCCSRGRSTSALSLGESLVGKLGLAEGGDNRKLYAAWRHSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAG7</accession>
    <name>A0A1A0HAG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_78183</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711634.1">
        <property type="nucleotide sequence ID" value="XM_018858840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAG7"/>
    <dbReference type="STRING" id="869754.A0A1A0HAG7"/>
    <dbReference type="GeneID" id="30031816"/>
    <dbReference type="OrthoDB" id="1879366at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08297">
        <property type="entry name" value="CAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000039">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000002">
        <property type="entry name" value="Alcohol dehydrogenase AdhP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42940:SF3">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="9">
        <location>
            <begin position="16"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="348" mass="36558" checksum="DF9ED9A8F34E88DA" modified="2016-10-05" version="1">MAIPQTQKAVVFDTNGGPLQYKDIAVPTPKENEILVHVKYSGVCHTDLHAWKGDWPLATKLPLVGGHEGAGEVVALGKNVKGWRLGDLAGIKWLNGSCLQCELCQHGNEPNCAEADLSGYTHDGSFQQYATADAIQAARLPQGTDLAQAAPILCAGITVYKALKTAALQAGQWVCISGAGGGLGSLAIQYATAMGYRVVAIDGGDEKRDFCRSLGAEQFVDFTSSKDIVRDVVAATGGGAHDAINVSVSEKAISQSLEYLRPTGVAVLVGLPAGAKVVAPVFEAVVKSVSIKGSYVGNRADSAEAIDFFSRGLIKCPIKIVGLSELPKIYELMEAGKILGRYVVDTSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAJ2</accession>
    <name>A0A1A0HAJ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711659.1">
        <property type="nucleotide sequence ID" value="XM_018855907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAJ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HAJ2"/>
    <dbReference type="GeneID" id="30028883"/>
    <dbReference type="OrthoDB" id="159449at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="129"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="536" mass="60692" checksum="F3F8ABF8FB4E7D14" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAL3</accession>
    <name>A0A1A0HAL3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein CSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_11635</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711561.1">
        <property type="nucleotide sequence ID" value="XM_018854044.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HAL3"/>
    <dbReference type="GeneID" id="30027020"/>
    <dbReference type="OrthoDB" id="10051416at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006113">
        <property type="term" value="P:fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029636">
        <property type="entry name" value="Csf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056779">
        <property type="entry name" value="Csf1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048636">
        <property type="entry name" value="Csf1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32085:SF3">
        <property type="entry name" value="PROTEIN CSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25038">
        <property type="entry name" value="Csf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21678">
        <property type="entry name" value="Csf1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 N-terminal" evidence="3">
        <location>
            <begin position="171"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="domain" description="Csf1 C-terminal region" evidence="4">
        <location>
            <begin position="1760"/>
            <end position="2914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2808"/>
            <end position="2829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2914" mass="326475" checksum="B5D735661D43B91B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ3</accession>
    <name>A0A1A0HAQ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_11764</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711714.1">
        <property type="nucleotide sequence ID" value="XM_018854078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAQ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAQ3"/>
    <dbReference type="GeneID" id="30027054"/>
    <dbReference type="OrthoDB" id="9446342at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF8">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="446" mass="50333" checksum="12F4764CA8B6128A" modified="2016-10-05" version="1">MDENYDVIVLGTGLTECVLSGILSVEGKKVLHIDRQDFYGGESASLNLLQLYQKFKPSSQKPDSITGRDRDWCVDLIPKFLMSNGELTNILVHTEVTKYIEFKQIGGSYVYRSGKISKVPSNEMEALRSSLMGIFEKRRMKRFLEFVAAYDDDKLSTHQGLDLDKNSMDEVYTHFGLERGTKDFIGHAMALWANDDYLTELARPTIERIILYVQSVAKYGKSPYIYPLYGLGELPQGFARLSAIYGGTYMLDTPIDEVLYDENNQFKGVVTKEGTAKAPIVIADPTYFPDRVKKTGHKVIRAMCILDHPVPNTNNLESVQIIIPQNQVGRQNDIYIAVLSDEHCVVPKGYYLAIVSTIVETSSPHVELEPAFKLLGNRIDTLMGLAELYEPVEDGSESNIFISKSYDPSSHFETTTDDIKDIYFRVTGNPLVLKKRPTDEELENLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAX3</accession>
    <name>A0A1A0HAX3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_179467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711784.1">
        <property type="nucleotide sequence ID" value="XM_018854747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAX3"/>
    <dbReference type="GeneID" id="30027723"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="83" mass="9498" checksum="DF4A4D6EEFD07C7D" modified="2016-10-05" version="1">MHIIVFEVQVHCEVTDFLFLSTLATVSSACSLFISLLHYVFVSYTILIVSWLLHCTFGMLPYFPLLPFLILILWLFVLGPFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB36</accession>
    <name>A0A1A0HB36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_82964</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711849.1">
        <property type="nucleotide sequence ID" value="XM_018859225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB36"/>
    <dbReference type="GeneID" id="30032200"/>
    <dbReference type="OrthoDB" id="4085087at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="209" mass="23055" checksum="7AAC8CD3B2C378DC" modified="2016-10-05" version="1">MNINISTEIVKSVTNKTKDPWNLAEGDHLGLLAPVAIDPVDMLSSIDIKDVTESVAEILMILIKKLADELLLPFLRVLRVVLESLLGDKSPLVIIWIENFVRSVVEVIFKALFVGQESSKVEMWTASLTEDLLKALLGIAKELDLDNTLADPSLNSNAAVLSAKEVSYILLIPLITSKSKYEDSVEDSVDDSLKATIEDFKAHIEKALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBD1</accession>
    <name>A0A1A0HBD1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11744</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711692.1">
        <property type="nucleotide sequence ID" value="XM_018854076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBD1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBD1"/>
    <dbReference type="GeneID" id="30027052"/>
    <dbReference type="OrthoDB" id="4093878at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="339"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="386" mass="44150" checksum="C29BE3968C735EEC" modified="2016-10-05" version="1">MLTCITPSSAIFLDALMHNVQQTQYLECAILQKPKYIKRVETDDLYQIQLFKDLGNFRSYKLRVSADSRNPFVVNLVVESFVDGFKKVFQFLSKDIDIDRISWQQYERHSALILNVPKRRQIFVDLFSVMPSNLTFTTTPKADSKKSLKNTDFAGREVPVQVKMDIPNQITQTANINIEKLNRAREYYRDDTSNQFSTPKREIASTEHGAFEAVKQAQLKQKRKCAHETHNESISHAAKKDGEKISKLSKFEKQQQLNKEEAQKYLYALIGGALITGKHGLNLGDNETKKATGNLNFETEVSSCSCQSNESNEYSFVPGSSSELSASAENSISQDEVENTNQETESINSEECERLVDDDTNYLKRHPSMEEVEDEEFVFLRKKFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBF0</accession>
    <name>A0A1A0HBF0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PR-1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31786</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711723.1">
        <property type="nucleotide sequence ID" value="XM_018855917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBF0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBF0"/>
    <dbReference type="GeneID" id="30028893"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01009">
        <property type="entry name" value="CRISP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCP" evidence="2">
        <location>
            <begin position="136"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="268" mass="27989" checksum="29DAC612D7D06053" modified="2016-10-05" version="1">MAAAKTVSITRYHYVTVDMSGNIIGSSIVLSQQSQSTAPVTVSAFDDVAAVGATTATTVETYSPSLVNLGFDLEETTIVTSPTVSTIATSQTNSDAAIETNQQATKTSQATSASVSTGDSESSIYSEISSSGVDAEFAKAILDAHNEKREAHSASGLSWSANLFTYAQNYADSYTCGGSLVHSGDSYGENLAYGYSTGVAALEAWYSEGTGYDYSTSTVLDHFTQVIWQSTTELGCAYKSCSGGLYVICSYNPAGNYVGEGLENLSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBG1</accession>
    <name>A0A1A0HBG1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_70082</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711984.1">
        <property type="nucleotide sequence ID" value="XM_018858385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBG1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBG1"/>
    <dbReference type="GeneID" id="30031361"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046943">
        <property type="term" value="F:carboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1720.10:FF:000013">
        <property type="entry name" value="Related to multidrug resistance proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF78">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01036">
        <property type="entry name" value="TCRTETB"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="334"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="542"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="71"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="589" mass="64503" checksum="E754F49914F0B549" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBH7</accession>
    <name>A0A1A0HBH7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711850.1">
        <property type="nucleotide sequence ID" value="XM_018855938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBH7"/>
    <dbReference type="GeneID" id="30028914"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="59" mass="6808" checksum="10D0ADCE97359D63" modified="2016-10-05" version="1">MLAPADNSLENLTKLNWFVTTIFKVYTRRRGESQEVGSLSPLQPAELKRLLQETYPRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBW7</accession>
    <name>A0A1A0HBW7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41957</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711893.1">
        <property type="nucleotide sequence ID" value="XM_018857470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBW7"/>
    <dbReference type="STRING" id="869754.A0A1A0HBW7"/>
    <dbReference type="GeneID" id="30030446"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="6">
        <location>
            <begin position="21"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="5">
        <location>
            <begin position="211"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="342"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="437" mass="48241" checksum="F37542CEBCF07890" modified="2016-10-05" version="1">MTGGTSDTGAPDAIDQATQHRYRVFKKNSPYLYDYLCTNSLLWPSLSVQFFPDLEVPDANAPGATVPPPAAPKLQLSTQLAHQRLLLGTFTLGQAVDAIHLYQVPYYRNLNRCINTDHWAYNSEKEEFELPTVSKTKPRVLQLINHHGDVNRLRYMPQNPDVLASANNVGDLSVYNRTKHATIRKLGEELRINEPQLRLVNSSGARATDIFALDWNRQAQSVVVSASMDGQINVFDVQGNYAARSSANVIHETWTAQHAAAINDIEWAAKHDAVFLAADDAGALSVFDTRAPGTPLQTLAGSVPKNSVSINPSSDFCVASGDSQGQIEVWDLRALLQSARVLPAHLDAITQVKWHPRFPAILGSSSADRLVKIFDMSEPADALLFSHEGHMLGVNDFDWSLHEDWMVASVADDNSLHLWKPAHHVFKKLYAEGLPGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC89</accession>
    <name>A0A1A0HC89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_12108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712131.1">
        <property type="nucleotide sequence ID" value="XM_018854170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC89"/>
    <dbReference type="STRING" id="869754.A0A1A0HC89"/>
    <dbReference type="GeneID" id="30027146"/>
    <dbReference type="OrthoDB" id="5551751at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040200">
        <property type="entry name" value="Mug57-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28156:SF1">
        <property type="entry name" value="FAS1 DOMAIN-CONTAINING PROTEIN YDR262W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291737" description="FAS1 domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="3">
        <location>
            <begin position="80"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="230" mass="25013" checksum="3D57CA45DAF52B70" modified="2016-10-05" version="1">MKLVNLAVLAFASCILAKNIVYFDALVSAMNNDKREAKNIVSLDALKAASGSGVYEEEAAGNADPAARKRDEQDVFHMSAPLLQSLLPQVPQISVFSSYLRNDARMASQMENAGSFVLIVAPTDEAFARFSASTGKKPWEFPQEVRDDDTDDQVISANLRHFVEAHVSRPIDRELAENAPITLLSGEEMTIERLAGPDSYQVTVGDKQAAVASIRQAENGVVFVVDTLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG3</accession>
    <name>A0A1A0HCG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_188240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712087.1">
        <property type="nucleotide sequence ID" value="XM_018854812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG3"/>
    <dbReference type="GeneID" id="30027788"/>
    <dbReference type="OrthoDB" id="4083463at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019433">
        <property type="entry name" value="GPI_ManTrfase_II_coact_Pga1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10333">
        <property type="entry name" value="Pga1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291745" evidence="2">
        <location>
            <begin position="17"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="216" mass="24235" checksum="10952A164E458B00" modified="2016-10-05" version="1">MLVLAVVLSLGRLVCANTEAYGFQIPNYYDVPGHAPGGPASTAIVPLNHTARLLTDHPILSVSNYSTAELVLTVPYDFSGLPKQTLFVRLNNYHNCSFEPNDLIHVKLCWPATTPVNFNLSHRFIQEREFGAEASPRDTLHIYLVIDYQADFYPVEPMHHTSFDMLLAVSKLPNTVPIPIELYGVIVYVVDLMILTATALPWILQQFSSYIELMQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCG4</accession>
    <name>A0A1A0HCG4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UV excision repair protein RAD23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_77371</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Involved in nucleotide excision repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RAD23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712300.1">
        <property type="nucleotide sequence ID" value="XM_018858682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCG4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCG4"/>
    <dbReference type="GeneID" id="30031658"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120125">
        <property type="term" value="C:PNGase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000224">
        <property type="term" value="F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01805">
        <property type="entry name" value="Ubl_Rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000002">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000003">
        <property type="entry name" value="UV excision repair protein RAD23 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.540">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004806">
        <property type="entry name" value="Rad23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015360">
        <property type="entry name" value="XPC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036353">
        <property type="entry name" value="XPC-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00601">
        <property type="entry name" value="rad23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10621:SF0">
        <property type="entry name" value="UV EXCISION REPAIR PROTEIN RAD23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09280">
        <property type="entry name" value="XPC-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01839">
        <property type="entry name" value="RAD23PROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101238">
        <property type="entry name" value="XPC-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="8">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="132"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="7">
        <location>
            <begin position="309"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="80"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="80"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="352" mass="37172" checksum="4C5139D65E1A0BEE" modified="2016-10-05" version="1">MKIKFRDLKKLTVEVDAQPSDSVLSVKEKVAAAKDVDALQLKFVYSGKVLQDDKLVLDFKIKDGDSVITMVSKAKVATNTAPTNTAPTNTAPTNTGATNSTATNSTATNTAATTTAPGGSQSPSSAADFAAGLEREAAVQNMMEMGYDRPEIERALRAAFNNPHRAVEYLLSGIPASLQAPEPAEAGDAEADRAGPSVDEPADAESGHQNLFDAAAAASTDDVAPDDGLGGMSEEGQLALLREAIQSNPELLQPLLERMAATDPQAAAMIAEDPEGFVHRFLSGELGMDVDDADGGDDPEGVVRVELSELDQQAIARLCELGFDRNLVIQVYMACDKNEEVTADILFRDTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCM0</accession>
    <name>A0A1A0HCM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_41025</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712147.1">
        <property type="nucleotide sequence ID" value="XM_018857344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCM0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCM0"/>
    <dbReference type="GeneID" id="30030320"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="7">
        <location>
            <begin position="53"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="6">
        <location>
            <begin position="177"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="57"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="440"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="823" mass="93946" checksum="9515CD1A87A46301" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCU3</accession>
    <name>A0A1A0HCU3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712222.1">
        <property type="nucleotide sequence ID" value="XM_018855998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCU3"/>
    <dbReference type="GeneID" id="30028974"/>
    <dbReference type="OrthoDB" id="4084022at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10175">
        <property type="entry name" value="MPP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="132" mass="14857" checksum="4369F757DDF2FBBC" modified="2016-10-05" version="1">MGLSSNVMNMKFMQKAQNSKKNDLHELETKKIRDLSEWLLPSGTVKPKIKPAVTVRTVGYGSIASLTAKENDAISNDNPKSQTAPPSNEPEKTRKDDAQTLLNEILDKAQKDQKRLQKQSKQLRKKARKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD54</accession>
    <name>A0A1A0HD54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712359.1">
        <property type="nucleotide sequence ID" value="XM_018855728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD54"/>
    <dbReference type="GeneID" id="30028704"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="114" mass="13048" checksum="0743545316C64A4A" modified="2016-10-05" version="1">MLLARIKPSEFHGILSQFSVLAKSFTLVVLAFRGTWPEIAKVARGFKRSLRSWCSNQATPHQAPDDVQAPKAPFYVQRCRSPKGSFFYVQRLVANARLQLTRESQWVIRLEARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDF5</accession>
    <name>A0A1A0HDF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Isocitrate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_10843</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Also acts on 2-methylisocitrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-threo-isocitrate = glyoxylate + succinate</text>
            <dbReference type="Rhea" id="RHEA:13245"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="4.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Can also use Mn(2+) ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712423.1">
        <property type="nucleotide sequence ID" value="XM_018853860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDF5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDF5"/>
    <dbReference type="GeneID" id="30026836"/>
    <dbReference type="OrthoDB" id="4078635at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.850:FF:000001">
        <property type="entry name" value="Isocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631:SF3">
        <property type="entry name" value="BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="ISOCITRATE LYASE/MALATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="8 11 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="430"/>
            <end position="434"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00057462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="551" mass="61606" checksum="F058B0E803099067" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDH2</accession>
    <name>A0A1A0HDH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor IF2/IF5 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_40109</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712562.1">
        <property type="nucleotide sequence ID" value="XM_018857232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDH2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDH2"/>
    <dbReference type="GeneID" id="30030208"/>
    <dbReference type="OrthoDB" id="10255414at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990856">
        <property type="term" value="F:methionyl-initiator methionine tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="5">
        <location>
            <begin position="153"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="284" mass="30966" checksum="B9F44E4B312720E8" modified="2016-10-05" version="1">MSDLGFDPSLKKKKKSKKTDDDAAAAASSTPAPADMDDMFAGLKKKKKAKKSADGVDESAADGSALSVDEISESLGDLGLKKKKKKSAKLATASEFDHALEEAGLDETAGTVSVETEETLAQAKLGLPYGDLLLRFFTILKQNNPELAGDRSGPKFKIPPPVCQREGSKKTLFANVQDIATVLQRNPEHLIQFLFAELGTSGSIDGEKRLVLKGKFQPKQMESVLRRYIIEYVTCKTCKSMNTELKRESANRLHFLSCKACCSTRSVSSIKTGFQAQIGRRKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDK8</accession>
    <name>A0A1A0HDK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_145584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712556.1">
        <property type="nucleotide sequence ID" value="XM_018854518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDK8"/>
    <dbReference type="GeneID" id="30027494"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="125" mass="13959" checksum="F1A7ACD7DE3522D5" modified="2016-10-05" version="1">MGRKLTRETRQGITRMLLGYTGCFHLGLLLASTGGRLHFRFAHCRVQIFTKSAYVAQAQKLSEGLNTILGGILGAPSSDYFPTHYSAVLNLCWSLNGIFQIPVSRNVFGKLWLRLCAQLRSTLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDM9</accession>
    <name>A0A1A0HDM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation machinery-associated protein 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_77521</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMA20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712498.1">
        <property type="nucleotide sequence ID" value="XM_018858716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDM9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDM9"/>
    <dbReference type="GeneID" id="30031692"/>
    <dbReference type="OrthoDB" id="10249667at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11609">
        <property type="entry name" value="MCT1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21155">
        <property type="entry name" value="PUA_MCTS-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041366">
        <property type="entry name" value="Pre-PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17832">
        <property type="entry name" value="Pre-PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="91"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="19778" checksum="FD659D02968D7C81" modified="2016-10-05" version="1">MFKKFGKAEIHSRSNIKLSAQRGLKSSFTAQYEDLEPVIDSIIPKKSQAILIKCEEKILLYSVDGEVVVFQTFGDLVPTLKIVHKYPDCFPKVKVDRGAIKFVLSGANVMCPGLTSAGAQLPAGNLEKDAIVTIYAEGKDTALAVGRLLMSTDDIKSINKGIGIELLHYLGDGLWYLDESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDR6</accession>
    <name>A0A1A0HDR6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ER membrane protein complex subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_39323</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMC6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712725.1">
        <property type="nucleotide sequence ID" value="XM_018857129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDR6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDR6"/>
    <dbReference type="GeneID" id="30030105"/>
    <dbReference type="OrthoDB" id="16510at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072546">
        <property type="term" value="C:EMC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045050">
        <property type="term" value="P:protein insertion into ER membrane by stop-transfer membrane-anchor sequence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008504">
        <property type="entry name" value="Emc6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029008">
        <property type="entry name" value="EMC6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20994:SF0">
        <property type="entry name" value="ER MEMBRANE PROTEIN COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07019">
        <property type="entry name" value="EMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="108" mass="12092" checksum="CC8CC42776224CB9" modified="2016-10-05" version="1">MSTANKDIEQVYYDAHLVSNKYKLQKLQDVMSLSLGIAAGTLGLESYSGIAFYLVGILASDFSFLVVCCEGTPKRFFVSPVREIFADNFISNFAGYIMMWCLTYALMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDV3</accession>
    <name>A0A1A0HDV3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712656.1">
        <property type="nucleotide sequence ID" value="XM_018855770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDV3"/>
    <dbReference type="GeneID" id="30028746"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="54" mass="6128" checksum="ABD664E2A2BBEAC0" modified="2016-10-05" version="1" fragment="single">MGETTMSAETNKDTSTLTFNPTMYFSDAEQDRAAQEYLSNMNRLLETKNLSITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDY6</accession>
    <name>A0A1A0HDY6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha,alpha-trehalase</fullName>
            <ecNumber evidence="3">3.2.1.28</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_39797</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 65 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712722.1">
        <property type="nucleotide sequence ID" value="XM_018857178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDY6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDY6"/>
    <dbReference type="GeneID" id="30030154"/>
    <dbReference type="OrthoDB" id="200349at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030287">
        <property type="term" value="C:cell wall-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000328">
        <property type="term" value="C:fungal-type vacuole lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015771">
        <property type="term" value="P:trehalose transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000032">
        <property type="entry name" value="Vacuolar acid trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.40">
        <property type="entry name" value="Glycoside hydrolase, family 65, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037018">
        <property type="entry name" value="GH65_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005195">
        <property type="entry name" value="Glyco_hydro_65_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005196">
        <property type="entry name" value="Glyco_hydro_65_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11051:SF8">
        <property type="entry name" value="PROTEIN-GLUCOSYLGALACTOSYLHYDROXYLYSINE GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03632">
        <property type="entry name" value="Glyco_hydro_65m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03636">
        <property type="entry name" value="Glyco_hydro_65N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291762" description="alpha,alpha-trehalase" evidence="6">
        <location>
            <begin position="26"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 N-terminal" evidence="8">
        <location>
            <begin position="76"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 65 central catalytic" evidence="7">
        <location>
            <begin position="402"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1040" mass="117023" checksum="DBF39B36CAB9845F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE05</accession>
    <name>A0A1A0HE05_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Barwin-like endoglucanase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_25703</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712628.1">
        <property type="nucleotide sequence ID" value="XM_018855299.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE05"/>
    <dbReference type="STRING" id="869754.A0A1A0HE05"/>
    <dbReference type="GeneID" id="30028275"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF28">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="RlpA-like protein double-psi beta-barrel" evidence="2">
        <location>
            <begin position="44"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="102" mass="11240" checksum="13005B0289643E1E" modified="2016-10-05" version="1" fragment="single">YSGDGTYYDTGLGACGETNTNDDYIVALSHVLFDQYTPGTNPNDNTLCGREIIASYEGKSVKVTAVDRCEGCLEYDLDFSPAAFKQLADPDLGRIKITWEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE80</accession>
    <name>A0A1A0HE80_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fimbrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_11074</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712703.1">
        <property type="nucleotide sequence ID" value="XM_018853914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE80"/>
    <dbReference type="STRING" id="869754.A0A1A0HE80"/>
    <dbReference type="GeneID" id="30026890"/>
    <dbReference type="OrthoDB" id="431378at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099079">
        <property type="term" value="C:actin body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031097">
        <property type="term" value="C:medial cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120106">
        <property type="term" value="C:mitotic actomyosin contractile ring, distal actin filament layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044396">
        <property type="term" value="P:actin cortical patch organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051639">
        <property type="term" value="P:actin filament network formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044837">
        <property type="term" value="P:actomyosin contractile ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070649">
        <property type="term" value="P:formin-nucleated actin cable assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21294">
        <property type="entry name" value="CH_FIMB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21300">
        <property type="entry name" value="CH_FIMB_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000042">
        <property type="entry name" value="Fimbrin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000010">
        <property type="entry name" value="Plastin-3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000016">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000027">
        <property type="entry name" value="Probable fimbrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001589">
        <property type="entry name" value="Actinin_actin-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039959">
        <property type="entry name" value="Fimbrin/Plastin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961:SF18">
        <property type="entry name" value="FI19014P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19961">
        <property type="entry name" value="FIMBRIN/PLASTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00020">
        <property type="entry name" value="ACTININ_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="146"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="295"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="419"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="6">
        <location>
            <begin position="541"/>
            <end position="651"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="653" mass="72728" checksum="A8AA6FA95276BFF2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HES9</accession>
    <name>A0A1A0HES9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="8">PPIase</shortName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_39631</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713002.1">
        <property type="nucleotide sequence ID" value="XM_018857159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HES9"/>
    <dbReference type="STRING" id="869754.A0A1A0HES9"/>
    <dbReference type="GeneID" id="30030135"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01926">
        <property type="entry name" value="cyclophilin_ABH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000032">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="21"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="180" mass="19568" checksum="1D8526016B0E25B7" modified="2016-10-05" version="1">MLKTSVRSLSSTSRVLGTKVFMTTSIGGHKQNPIKFELFDEVVPKTAENFRALCTGEKGFGYANSSFHRVIPQFMLQGGDFTRHNGTGGKSIYGEKFADENFSKKHTKPGLLSMANAGPNTNGSQFFITTVPCPWLDGAHVVFGEVIEGMDVVEKIEAQGSQTGSPRSKIVIEECGELTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEU7</accession>
    <name>A0A1A0HEU7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyisobutyryl-CoA hydrolase</fullName>
            <ecNumber evidence="2">3.1.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_40115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-hydroxy-2-methylpropanoyl-CoA + H2O = 3-hydroxy-2-methylpropanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20888"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57340"/>
            <dbReference type="EC" id="3.1.2.4"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712908.1">
        <property type="nucleotide sequence ID" value="XM_018857233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEU7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEU7"/>
    <dbReference type="GeneID" id="30030209"/>
    <dbReference type="OrthoDB" id="1737613at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000080">
        <property type="entry name" value="3-hydroxyisobutyryl-CoA hydrolase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004127">
        <property type="entry name" value="PRK05617.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176:SF3">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl-CoA hydratase/isomerase" evidence="4">
        <location>
            <begin position="21"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="469" mass="52136" checksum="DB9D2C9D80D74DBF" modified="2016-10-05" version="1">MASTSGHVADDVLFTNKNMARVITLNRTHKLNSLNTLMVLKIVPRLTEYAKLDVCNSIILTSSSPKALCAGGDVAECALQILQGNPLYAMDFFQQEYNMNFLIATYSKPCVAIMDGVTMGGGVGLSVHAPFRVATENTKLAMPEMDIGFFPDVGTTFFLPRLDDKLGYYYALTGEVLSGADAYMAGFATHYVPAERLSQMTTRISNLNPPVINGSANEASNLRSQKDFFVQMNSVIEEFALTKLPENYKFHLSAEDISLINKSFSQKSFEDVLASFKNSGTEFGLKTYERLAAKSPTCVRVAFELLNRGLENTLQKQLELELITSTNMMNAKPEVNDFVKGVKHKLLDKIKEPSLPEWAAYDASRVQKLTSSSVYTAMLSKPMINNFFGVNFKNYIFNMGLPTNGQIHDYIVGQDGSNRSYLPTPSEVVKHFEAKTHQKLGVADKVKRVLAVHGDSSQYDNKYVTWKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFF1</accession>
    <name>A0A1A0HFF1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DUF500-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_76032</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SH3YL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713355.1">
        <property type="nucleotide sequence ID" value="XM_018858464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFF1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFF1"/>
    <dbReference type="GeneID" id="30031440"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11842">
        <property type="entry name" value="SH3_Ysc84p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11525">
        <property type="entry name" value="SYLF_SH3YL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000100">
        <property type="entry name" value="SH3 domain-containing YSC84-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033643">
        <property type="entry name" value="SYLF_SH3YL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF2">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING YSC84-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="5">
        <location>
            <begin position="356"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="237"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="331"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="415" mass="44601" checksum="2CDDFA52D2AADE6E" modified="2016-10-05" version="1">MGINNPIPRSLKSESKKAAKVLASFIKPNQVAGPEQVIPPEVLRNAKGLAIITVLKAGFLFSGRAGSGVIVARLPDGSWSPPSAIVTAGAGVGGQIGAELTDFVFILNTRSAVESFAQMGSITLGTNLSVAAGPLGRNAEAAGTASLKSVSAVFAYSKTKGLFAGVSLEGSAILERRETNRKFYGSNCKARNILAGQVEAPAGCEPLMRVLESRVFSNRLSDDDSFNDDYYDDIPDEFSDSTSDYSRNDRVRYQGSRRYSDEYGYSDDEYSDDHLRQRRHSTNNRTSQGTSAGGTSRRSTWEDNIYDGGSSRRRTNSEVDNLGNRLASTKLAGSTVSPSRPSANSKPNFGGAAPKSYANQAIALYLFKGEQPGDLPFKKGDVIDILKKSETTDDWWTGRNNGVSGIFPANYVELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFG3</accession>
    <name>A0A1A0HFG3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_37000</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713206.1">
        <property type="nucleotide sequence ID" value="XM_018856810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFG3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFG3"/>
    <dbReference type="GeneID" id="30029786"/>
    <dbReference type="OrthoDB" id="196858at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037850">
        <property type="entry name" value="RBBP5/Swd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44040:SF1">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="65"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="430" mass="47875" checksum="DAD66DFB679B5B3A" modified="2016-10-05" version="1">MNLALQDPFAVAKEYPDTLAHTLQYGHSVHILFNRKGDYIALGLSDGTIVIYDLDSLAITCVLGRGGHVRPVTSLSWSHCGRYLLLASQDWSCILWDLRRANGAASAVLHKAQFDGPVWLACLHPKNPHVFVALLFENDPVCVHVDGVGGSRVTCLATEPAAARADDDDDTPKVLAGKHRTLAAVFSTSGDYILAGTSKGWLNVICTRRAATVHLVKVTSANIKSLLVASNGRKLAVNSSDRVIRQIHLPDLINEPNPAHWDFDVECKYQDMVNKLQWNSVAFSHNSDFLVASTFGQSSQDLYLWETSMGSLLKILEGSPEELIDVKWNYNRCMIASTGLDSGTVYLWLVQFPLKWSALAPDFVEIEENEDYEEKEDEFDIIDETDLIKKRMEEEDTDIDIFLLEATDVRGFDTQIDSFVIPLNYETAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY2</accession>
    <name>A0A1A0HFY2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713392.1">
        <property type="nucleotide sequence ID" value="XM_018855413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY2"/>
    <dbReference type="GeneID" id="30028389"/>
    <dbReference type="OrthoDB" id="1667587at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170071">
        <property type="term" value="C:CROP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070772">
        <property type="term" value="C:PAS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044090">
        <property type="term" value="P:positive regulation of vacuole organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="360"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="528"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="381"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="595" mass="65630" checksum="105D8237F20D9CB8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG52</accession>
    <name>A0A1A0HG52_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PSP1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_38025</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713446.1">
        <property type="nucleotide sequence ID" value="XM_018856945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG52"/>
    <dbReference type="GeneID" id="30029921"/>
    <dbReference type="OrthoDB" id="243127at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="2">
        <location>
            <begin position="383"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="582" mass="64598" checksum="6D0662624C0398C3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG57</accession>
    <name>A0A1A0HG57_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_96030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713467.1">
        <property type="nucleotide sequence ID" value="XM_018859417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG57"/>
    <dbReference type="GeneID" id="30032392"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="20611" checksum="4086D8298FB43C26" modified="2016-10-05" version="1">MPAEGNGRATSYLIYNVIVELKEIYRVGSRYADVCGSDIYLFSFWNFSFYAPMDIKKSKLKLDFYLDKIRNKECPDILDWGQNLNKEYVAHCTTRGYPNYHSFVSNQGEQECRDFTEHRIMYYLITRLVKKIEGYPEICTKNSKRNPRFVDSKGESKRPRKVWNKNLKGDSRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGG3</accession>
    <name>A0A1A0HGG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">1,3-beta-glucanosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_15547</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Splits internally a 1,3-beta-glucan molecule and transfers the newly generated reducing end (the donor) to the non-reducing end of another 1,3-beta-glucan molecule (the acceptor) forming a 1,3-beta linkage, resulting in the elongation of 1,3-beta-glucan chains in the cell wall.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Lipid-anchor</topology>
            <topology evidence="10">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the glycosyl hydrolase 72 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713424.1">
        <property type="nucleotide sequence ID" value="XM_018854609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGG3"/>
    <dbReference type="STRING" id="869754.A0A1A0HGG3"/>
    <dbReference type="GeneID" id="30027585"/>
    <dbReference type="OrthoDB" id="421038at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042124">
        <property type="term" value="F:1,3-beta-glucanosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071970">
        <property type="term" value="P:fungal-type cell wall (1-&gt;3)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004886">
        <property type="entry name" value="Glucanosyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31468:SF5">
        <property type="entry name" value="1,3-BETA-GLUCANOSYLTRANSFERASE GAS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03198">
        <property type="entry name" value="Glyco_hydro_72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 10" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="9 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444437" description="1,3-beta-glucanosyltransferase" evidence="10">
        <location>
            <begin position="18"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="383"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="390"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="429" mass="46111" checksum="FE481FD4604C5D9B" modified="2016-10-05" version="1" fragment="single">FLLLLAPSLAAFLPIAAEGNALFVNGTSERFYIRGIDYQPGGSSKLIDPIANVDNLKRDIPYFEDLGINTIRAYSIDNTADHSEGMKLLLDAGIGLILDTNIPLALIARDDGAECSYNTMYLNEVLATVRIMSAYDNLIGIFAANEVINDLDTTPAAAYVKAVIRDIKTFQRNTGLRTVPVGYSAADIEENRVQSAHYFNCGDDEMARADFYGFNDYSWCGDSSFTISGYDKKVETFLNHSIPLFFSEYGCNKVTPRKFTEVESIYSTDMTHVFSGGLVYEYSQEANDYGLVELSSDNKTVTTKDDFDNLKLQFASVPNPSGDGGYQSDLPHSNCPSRSSVWEASNTIPDTPKGALKYINGVVEPEGNGFDADTQWACIAAGNDVDDSDSASSGSSSGSSSTRSGSSSGSSSTRSGSSTGSSSSNTASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGI0</accession>
    <name>A0A1A0HGI0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PA14 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713581.1">
        <property type="nucleotide sequence ID" value="XM_018859383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGI0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGI0"/>
    <dbReference type="GeneID" id="30032358"/>
    <dbReference type="OrthoDB" id="3996163at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903561">
        <property type="term" value="C:extracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030446">
        <property type="term" value="C:hyphal cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030445">
        <property type="term" value="C:yeast-form cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030448">
        <property type="term" value="P:hyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008440">
        <property type="entry name" value="Agglutinin-like_ALS_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033504">
        <property type="entry name" value="ALS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018871">
        <property type="entry name" value="GLEYA_adhesin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037524">
        <property type="entry name" value="PA14/GLEYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793:SF2">
        <property type="entry name" value="AGGLUTININ-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33793">
        <property type="entry name" value="ALPHA-AGGLUTININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05792">
        <property type="entry name" value="Candida_ALS"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10528">
        <property type="entry name" value="GLEYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56988">
        <property type="entry name" value="Anthrax protective antigen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51820">
        <property type="entry name" value="PA14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA14" evidence="2">
        <location>
            <begin position="248"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="758"/>
            <end position="779"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="927" mass="98372" checksum="F2EC3B9B02CEB87D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGL4</accession>
    <name>A0A1A0HGL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amine oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713474.1">
        <property type="nucleotide sequence ID" value="XM_018859356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGL4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGL4"/>
    <dbReference type="GeneID" id="30032331"/>
    <dbReference type="OrthoDB" id="5046242at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046592">
        <property type="term" value="F:polyamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046208">
        <property type="term" value="P:spermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF410">
        <property type="entry name" value="LYSINE-SPECIFIC HISTONE DEMETHYLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="1">
        <location>
            <begin position="14"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="487" mass="53591" checksum="D243445043A4EEF1" modified="2016-10-05" version="1">MAMHKKVCIIGAGVSGLRAAHKLLTAKDTLLLPSDVIILEAQARIGGRIKTDTTLSKLGLTYDLGAAWFHDALTNSVLYSSIEDGSFDVESDGYFNDKDMCYYAKEHPGRVDAAGLKLEQVVEDIEKFIEIHFLHSLGVPDVSLSEIVALYMKKQDCFLTAPQKQLCGRMCRYLELWYGISAHKMSGKHAVMSHQGRNLYNKKGYSFLVEKLQSQTKCTIMTSEQVTTIQRDISGQNFRHLVTTAAGTEIAADYLVVTVPQSILALDAEHSYGLTWDPPLPARIQQSLKTIHFGALGKVIFEFSEIWWDASEDAFEVLADENTGADVATASGLDISPFMYPIYVFNYATIQPSAASLVILTQSPVTEYLEAKPHLAWAYMKPMLAKISMQQICEPINTIVTDWTQNPYSRGSYSALHVGDDPSDAIIQLSGEYESCGLGISSTVRFAGEHTIADGAGCVHGAYDSGERAADWILKHLAENHQAKNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGU3</accession>
    <name>A0A1A0HGU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2470 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713697.1">
        <property type="nucleotide sequence ID" value="XM_018856741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGU3"/>
    <dbReference type="GeneID" id="30029717"/>
    <dbReference type="OrthoDB" id="5553410at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.10">
        <property type="entry name" value="PNP-oxidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019595">
        <property type="entry name" value="DUF2470"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037119">
        <property type="entry name" value="Haem_oxidase_HugZ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37783">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G04315)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37783:SF1">
        <property type="entry name" value="MEMBRANE PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G04315)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10615">
        <property type="entry name" value="DUF2470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2470" evidence="2">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="220" mass="25038" checksum="D08602BCCC9F45AF" modified="2016-10-05" version="1">MTDPAYRIKNHMNKDHHLALVDYVVVYGKERLSNIDVHSVHIAHVTVDELALKYKAKNGMEQDLKLEWNSVDDGEGLRVESMLDIKAKLIAMAKHAAQKQGFSHKQITKILPPKTVTPFVAYAFAALSVATYIKRDFLRSVLGLVFSQNSPGVESFLVFAEKRVLPIAGAMYAIHLLEMLFLVRPKTVKYRMPWPTKLAWMTFSFFEGYLCVPRLNSLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHF6</accession>
    <name>A0A1A0HHF6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha/beta-hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_29903</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713916.1">
        <property type="nucleotide sequence ID" value="XM_018855508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHF6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHF6"/>
    <dbReference type="GeneID" id="30028484"/>
    <dbReference type="OrthoDB" id="8119704at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46118:SF4">
        <property type="entry name" value="PROTEIN ABHD11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="59"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="321" mass="36591" checksum="C05678842CF9A021" modified="2016-10-05" version="1">MFFRRYFSHSAFRLTKVDILKQIELAKAHHAPELPVENSIALSSQDLSGKNEINDKKSPLIMIHGLFGARQNYSSVGRKISALSNRQTIGVDMRNHGASSHVFPHDYAHMAKDTIRYLEAFGRKVVLAGHLMGAKVSMLVALQRPELVEKLIVIDNSPKAETLDFRFTRDLLAMCRVEQELHSHGTTQARLLKDVDRIMAAYEPDALVRMFLASNLKRPSKKADNSEKVQFRIPVLNFLKHDVLPKMGGWPSELVRKHTFKKPVLVMRGLDSNFVADEHLKSLFPTYFPNFERVDFDSGHWLVSEQPDKFVQETLKFLGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHJ6</accession>
    <name>A0A1A0HHJ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar ATPase assembly integral membrane protein VMA21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_38478</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714115.1">
        <property type="nucleotide sequence ID" value="XM_018857008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHJ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHJ6"/>
    <dbReference type="GeneID" id="30029984"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03058">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019013">
        <property type="entry name" value="Vma21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09446">
        <property type="entry name" value="VMA21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7527" checksum="21632E222FBA6291" modified="2016-10-05" version="1" fragment="single">SVVQKLMFFTAAMVVLPLVTFFTCQYLFESSLVSGGSAAAAANLVLISYIVVAFAEDSGDSDEAPESKKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHL7</accession>
    <name>A0A1A0HHL7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Translocation protein SEC66</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713819.1">
        <property type="nucleotide sequence ID" value="XM_018856930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHL7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHL7"/>
    <dbReference type="GeneID" id="30029906"/>
    <dbReference type="OrthoDB" id="73168at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018624">
        <property type="entry name" value="Sec66"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229:SF1">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09802">
        <property type="entry name" value="Sec66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23319" checksum="E2E1A2C700F1A9C8" modified="2016-10-05" version="1">MAEEEKIEQVLKISVYTPLAYVAVMVTAFTIFSVLYRRHRLRKLIGIEPLFGPNHTLDLYVMIKDKFYDNLLPKDEKPPEKAVKAALLRRAVEAIRRSLKLKENEGVFNRLYQNGLIGEDTFQQFTMQNKVQQAELTEIVGECQQFSKEWPATFFPVAQEICYNEALRRRLNAMDSRSESLSTMWNYYVEKSEEAPKGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHV2</accession>
    <name>A0A1A0HHV2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_37745</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714220.1">
        <property type="nucleotide sequence ID" value="XM_018856908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHV2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHV2"/>
    <dbReference type="GeneID" id="30029884"/>
    <dbReference type="OrthoDB" id="5598305at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF101">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="436" mass="50189" checksum="D894E167157C07ED" modified="2016-10-05" version="1">MSDGNKPAAPSEPAKPAKKSWSNPALRAMGIPRVSLPSRNWMIFWTLLASVSGGIYYDKQQQKAIRQKYMKQVEALGQETYTTGRLPRKLSIFIAPPPDDFLDESLRHFRHYIKPILNAAAIDFEVYTENRQGDIRSQVAEKIRQLRRDQNAARLAESEELAQQAYEKSWSRFFRESVPLAFARFKRKEPEPETYVARHELYGPTDVLGLYKVAEPVTPHRDFEDDALLCGGVICIGRGTYKEYINGVHEGLLGPLEKPAEVVLNEKPQKTPEVADETKPAEIADFAEKEGEAAKKENPVPKPYILPGDYASGQLAPELDFNSVIRNKDNVPVIFEQPLYVFPIPKLTGFRNMPRKIYRYFTRRELAEEVSAKTMYVVDGHSRKFEFKDQFLGKEEEVEWPKKWVERGKEKKSEWVQELEVDERVTSRMRVYDSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIE8</accession>
    <name>A0A1A0HIE8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">FTHFS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_9862</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:23701"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:23702"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:22813"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:22814"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:20222"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:20223"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714094.1">
        <property type="nucleotide sequence ID" value="XM_018859474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIE8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIE8"/>
    <dbReference type="GeneID" id="30032449"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001741">
        <property type="entry name" value="Formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF26">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="16">
        <location>
            <begin position="28"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="17">
        <location>
            <begin position="181"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA23613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="988" mass="107051" checksum="8279EA362475C3D9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIH3</accession>
    <name>A0A1A0HIH3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_34567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714450.1">
        <property type="nucleotide sequence ID" value="XM_018856489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIH3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIH3"/>
    <dbReference type="GeneID" id="30029465"/>
    <dbReference type="OrthoDB" id="240546at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035658">
        <property type="term" value="C:Mon1-Ccz1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013176">
        <property type="entry name" value="Ccz1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13056:SF0">
        <property type="entry name" value="VACUOLAR FUSION PROTEIN CCZ1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="714" mass="81502" checksum="BF7133E7EC1C13CC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIJ3</accession>
    <name>A0A1A0HIJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_30224</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714316.1">
        <property type="nucleotide sequence ID" value="XM_018855578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIJ3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIJ3"/>
    <dbReference type="GeneID" id="30028554"/>
    <dbReference type="OrthoDB" id="205794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008409">
        <property type="entry name" value="SPF27"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05700">
        <property type="entry name" value="BCAS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="199" mass="22782" checksum="1CDDC3E9F4B74F27" modified="2016-10-05" version="1">MLSLDSDPLDYLPYIDGTITDTERQAVEQLIRAETPAEPGMLHSRLGELAPLSENRSLLMRDMERFELDYSDGLVPETFVSGGVSMDRYTDFGPDHDFSYDQMYTALLYSVLRDRNAQLAAEHSAERAHAQKEACESMRQVESLQRDLLVRKRERTEELNLAREAKQMQFQPVDGYLEQRWKDGIKGIVDTGIERHAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIV5</accession>
    <name>A0A1A0HIV5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_76851</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714417.1">
        <property type="nucleotide sequence ID" value="XM_018858599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HIV5"/>
    <dbReference type="GeneID" id="30031575"/>
    <dbReference type="OrthoDB" id="292693at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140104">
        <property type="term" value="F:molecular carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="38"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="188" mass="22119" checksum="BD02E29242829F68" modified="2016-10-05" version="1">MSQVIRARPLLQRRLLFGSSRGKDGFQTYRITKKISTHPDQLYLIITDVSKYEEFVPFVTSSFVNQYDPDTKLPTEAGLRVGWKQYDESFTCKLICEENKVIAESMSILLFESLYNEWNLKEVKSRFTNELSTLVELLLRYKFKNPFYNTLSSMFHNQVSNIMIKAFEERAMNLKIQSKLKDRMNNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIW3</accession>
    <name>A0A1A0HIW3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_30181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714259.1">
        <property type="nucleotide sequence ID" value="XM_018855572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIW3"/>
    <dbReference type="GeneID" id="30028548"/>
    <dbReference type="OrthoDB" id="4092598at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="164" mass="19314" checksum="3935102800EF7484" modified="2016-10-05" version="1">MRQWHSSPTRDLTSYEQIEQRQRTRADMLYQPMLSPTKNASQTSQKHKLREKMRQMRDDLREERKELSREKTVHDEDVRARLKLLQEQAELLEIDLDLLLQEEIETCEETSGKNNGTQITCRALAPSVAVSENTRHLSEFEEELEDLLAAEEMELEARFGDLQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIX2</accession>
    <name>A0A1A0HIX2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_113718</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714313.1">
        <property type="nucleotide sequence ID" value="XM_018853969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIX2"/>
    <dbReference type="GeneID" id="30026945"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="219" mass="24025" checksum="CEA549569BDE11F7" modified="2016-10-05" version="1">MAPVDAMFCVFQGALKLAVLYAFHSAVDLTLLYAFHGAVDLTVLCGFRSAVYRCFKHFTVPWTSRCFMHFTVPCTGALSISQCQSPKIQDFWIYGRYSLLEWPKFCGLVTVPRISSKSNDLTTFRWSCDVWVSGCFGDPQVLGGSWRNLTPSLALQSGSWIEDMAGTSPNGRGVSRTSEHLSGLLLIPACTHVIVATCCHWHHASTSPPTIVGVARSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ21</accession>
    <name>A0A1A0HJ21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Synaptobrevin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_77009</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714635.1">
        <property type="nucleotide sequence ID" value="XM_018858620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ21"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ21"/>
    <dbReference type="GeneID" id="30031596"/>
    <dbReference type="OrthoDB" id="190375at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15843">
        <property type="entry name" value="R-SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051097">
        <property type="entry name" value="Synaptobrevin-like_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136">
        <property type="entry name" value="SNARE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21136:SF168">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="4">
        <location>
            <begin position="192"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="287" mass="32074" checksum="37B6BDB279AF1C26" modified="2016-10-05" version="1">MTTRDLLKQLIYTTLTLNSTTLYATENTKLTALIPSLNCSELVSLELGLINASKTLIGLLSVVADRTVTKLSLLLYFMKKLLQNPDGGFDLVTVVVLAHQDVNKTLVLAVLKKIIDKYFEFRLDLACESITSEHAAKVKLGEFKLYMNQIIKFEEVQYDLNLALYSYGATRNGRDNNAGSPTPNSEIITPNQLVSANEDVDEVRLLMLDNINKILGRGDRIGTLVDQTERLQASSLILRKNAASVKRKMWFNNARSVVLIATVSVSCLYLVLGFECGYPFFQTCLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ77</accession>
    <name>A0A1A0HJ77_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">PLC-like phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_38433</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycerophosphoryl diester phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714690.1">
        <property type="nucleotide sequence ID" value="XM_018857000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ77"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ77"/>
    <dbReference type="GeneID" id="30029976"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034479">
        <property type="term" value="F:phosphatidylglycerol phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052271">
        <property type="entry name" value="GDPD-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42758:SF2">
        <property type="entry name" value="PHOSPHATIDYLGLYCEROL PHOSPHOLIPASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="GP-PDE" evidence="8">
        <location>
            <begin position="6"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="376" mass="44061" checksum="08570CFFD22BF6B0" modified="2016-10-05" version="1">MSSADCLVVGHRGFKSKFTENTLHGFQKCFDTGATIFETDVWTTKDEVLVISHDVNTKRVFCDENGNETDYNILETNFEQLKPLRTIGSGEPLLTFQDVLRWFCQVVKETANPNHKIMLDLKNANPPKLVRLLFEDMLAVHADLEWWFPRIQIGVWNLRFVKFLNQDDYFQRLFRGTNAYLGYTCFHIYHISLSWQSSLTFLAYNEYLDTLGKDRIKFKIAGVSLIYVSTWSREFLEDFMPAVKKQNLNLYTWTVNRVSQLRYFCAIGKVWKLKEYGVITDYPDQIVDALAGERWKNAEKQLLMESWPSGEQQNVVIPRSFMLFAWIAKIMTYFFHPAQLSVNQLFESPVLPHEKLYVRPKLGNRIFAFLQAWGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJB9</accession>
    <name>A0A1A0HJB9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">60S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_10408</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714735.1">
        <property type="nucleotide sequence ID" value="XM_018853763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJB9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJB9"/>
    <dbReference type="GeneID" id="30026739"/>
    <dbReference type="OrthoDB" id="528635at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000002">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3450:FF:000001">
        <property type="entry name" value="60S ribosomal protein L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045059">
        <property type="entry name" value="Ribosomal_uL29_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45722:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA24254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13512" checksum="9DC88C63AD95E31B" modified="2016-10-05" version="1">MAVVKVAELRSKSEEQLNQQLLELKQELASLKVQKVTKAVPQITVVRRNIARVLTVINANQRQSVRELYAGKKLLPKDLRAKKTRALRRALSKEDAARVTVKARKQQIAFPKKVYALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJC3</accession>
    <name>A0A1A0HJC3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amine oxidase</fullName>
            <ecNumber evidence="11">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_30458</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="11">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="10">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10 11">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="11"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714596.1">
        <property type="nucleotide sequence ID" value="XM_018855642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJC3"/>
    <dbReference type="STRING" id="869754.A0A1A0HJC3"/>
    <dbReference type="GeneID" id="30028618"/>
    <dbReference type="OrthoDB" id="5379943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.20:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000017">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049947">
        <property type="entry name" value="Cu_Am_Ox_Cu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF33">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01165">
        <property type="entry name" value="COPPER_AMINE_OXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="12">
        <location>
            <begin position="235"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="9">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="10">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="664" mass="74592" checksum="AC7300A645ABA281" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJM0</accession>
    <name>A0A1A0HJM0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pre-mRNA-processing protein 45</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_35647</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SNW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714691.1">
        <property type="nucleotide sequence ID" value="XM_018856638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJM0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJM0"/>
    <dbReference type="GeneID" id="30029614"/>
    <dbReference type="OrthoDB" id="666364at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017862">
        <property type="entry name" value="SKI-int_prot_SKIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12096">
        <property type="entry name" value="NUCLEAR PROTEIN SKIP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="5">
        <location>
            <begin position="144"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="291"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="322" mass="35686" checksum="3EF975E0D397CA08" modified="2016-10-05" version="1">MFSSLLPKPKHCSYDTIKVMLPKASQASSALVVPFSNTDSHKKVTNLHSSGNALGEVFVDANGFVDVGKTLASIDGDGGVQTSYDDTIPLKVKYPNLRHHFPRYTLENCPDDSLATCVAETKKVIDDILFKAENGEVSTDEPTFVTFTPSSLTENDARGRTLEIRNFKEDPLLPPKFKLRKNREKEPLPPPPILKAAPTEKITKEIKDKWHIPSAVSNWKNNQGFAIALDKRVHAASGGSASEGPSINIEKFGQLSLALENADKQARSELAARNEQRKQIALKEQAEKEKKLKELLDRSRRHSGKRTGNSHDYDGAKRYRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJN5</accession>
    <name>A0A1A0HJN5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_30553</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714711.1">
        <property type="nucleotide sequence ID" value="XM_018855660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJN5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJN5"/>
    <dbReference type="GeneID" id="30028636"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070682">
        <property type="term" value="P:proteasome regulatory particle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000067">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000007">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="212"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="434" mass="48265" checksum="C2318F10430E2251" modified="2016-10-05" version="1">MPGGGNLGKKKDDKKKDKPKYEPPVEAKFGKKRRKGPDTAVRLPSVYPTTRCKLKLLKLERIKDHLLLEEEFIANQEALQPTEARQAEEREKIDELRGYPMSIGTLEEIIDDDHAIVSSTAGSEYYVTIMSFVDKGLLEPGCSVLLHHKTVSVVGVLQDDADPMVSVMKLDKSPKESYADIGGLESQIQEIKEAVELPLTHPELYEEMGIKPPKGVILYGAPGTGKTLLAKAVANQTSATFLRIVGSELIQKYLGDGPRLCRQIFQIAGDLAPSIVFIDEIDAIGSKRYESSSGGEREIQRTMLELLNQLDGFDDRGDIKVIMATNKMESLDPALIRPGRIDRKILFENPDSNTKKKILSIHTSKMNLSSDVNLDELVTSKDDLSGADIKAMCTEAGLLALRERRMQVKAEDFRTAKERVLKNKVEENLEGLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJQ8</accession>
    <name>A0A1A0HJQ8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_76985</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714608.1">
        <property type="nucleotide sequence ID" value="XM_018858616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJQ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJQ8"/>
    <dbReference type="GeneID" id="30031592"/>
    <dbReference type="OrthoDB" id="2275560at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032545">
        <property type="term" value="C:CURI complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030291">
        <property type="term" value="F:protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006356">
        <property type="term" value="P:regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="256"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="261"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="285" mass="32169" checksum="D8EF87DE78225D42" modified="2016-10-05" version="1">MSTDAEGDYVPWIQQFCESFGHDYFVQVAQEFIEDDFNLTGLSLQVPYYREALYTILDYQVETAEHSAAGQAGEPRALGSGGKRQDLPNKVLLSHLAELLYGLIHARYIISKPGLTAMATKFEHLEFGYCPRYQCDSMHLVPVGATDVPGQETVRLYCPCCNDIYLPLSSRYLNIDGAFFGTTFPGLLVKMFPEIEHQCKNRIKRVNPDQTGIRLFGFRVHESSATGPRMRWLRQFPQTDDEKREFEMCELAMPAITASDVDVEESGNSEDGDSDDDKTMASEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK61</accession>
    <name>A0A1A0HK61_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase SUV3, mitochondrial</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_30802</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715050.1">
        <property type="nucleotide sequence ID" value="XM_018855714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK61"/>
    <dbReference type="STRING" id="869754.A0A1A0HK61"/>
    <dbReference type="GeneID" id="30028690"/>
    <dbReference type="OrthoDB" id="6692397at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000372">
        <property type="term" value="P:Group I intron splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006264">
        <property type="term" value="P:mitochondrial DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000957">
        <property type="term" value="P:mitochondrial RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001549">
        <property type="entry name" value="SUV3p ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="365"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="718" mass="81779" checksum="58DB619FC4F1FC4F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK82</accession>
    <name>A0A1A0HK82_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Alpha N-terminal protein methyltransferase 1</fullName>
            <ecNumber evidence="5">2.1.1.244</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">X-Pro-Lys N-terminal protein methyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_30689</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-alanyl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54712"/>
            <dbReference type="Rhea" id="RHEA-COMP:13785"/>
            <dbReference type="Rhea" id="RHEA-COMP:13971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138057"/>
            <dbReference type="ChEBI" id="CHEBI:138315"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-terminal L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-methionine = N-terminal N,N-dimethyl-L-prolyl-L-prolyl-L-lysyl-[protein] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54736"/>
            <dbReference type="Rhea" id="RHEA-COMP:13787"/>
            <dbReference type="Rhea" id="RHEA-COMP:13974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138059"/>
            <dbReference type="ChEBI" id="CHEBI:138318"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N-terminal L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N-terminal N,N,N-trimethyl-L-seryl-L-prolyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54724"/>
            <dbReference type="Rhea" id="RHEA-COMP:13789"/>
            <dbReference type="Rhea" id="RHEA-COMP:13973"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:138061"/>
            <dbReference type="ChEBI" id="CHEBI:138317"/>
            <dbReference type="EC" id="2.1.1.244"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. NTM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.244" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714892.1">
        <property type="nucleotide sequence ID" value="XM_018855684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK82"/>
    <dbReference type="STRING" id="869754.A0A1A0HK82"/>
    <dbReference type="GeneID" id="30028660"/>
    <dbReference type="OrthoDB" id="1298661at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071885">
        <property type="term" value="F:N-terminal protein N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008576">
        <property type="entry name" value="MeTrfase_NTM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753">
        <property type="entry name" value="AD-003 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12753:SF0">
        <property type="entry name" value="ALPHA N-TERMINAL PROTEIN METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05891">
        <property type="entry name" value="Methyltransf_PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016958">
        <property type="entry name" value="DUF858_MeTrfase_lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="137"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016958-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="248" mass="27885" checksum="43043BBC474E9C12" modified="2016-10-05" version="1">MPAKSADTAPDAHCENHDPNALIDYADAISYWLSVPASVDGVLGGFGEQTPVPKADIIGSATFLRKLQTRMQCPAGMPKLTIDMGAGIGRITRDFLWKVSDRCDLLEPVQPFVQQMRSELVPVAQKGRLGDIYDIGMQEWRCASEKRGLYWLVWCQWCVGQLPDEELVRFWARCREALMPNGTLIVKENIAPVDDIFDPTDLSVTRTDAKFRLLFQMAGYKLIASDVQKGMPRELYPVRMYCLKPLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKK0</accession>
    <name>A0A1A0HKK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endosomal/vacuolar adapter protein YPT35</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PX domain-containing protein YPT35</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_36764</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Recruits the lipid transfer protein VPS13 to endosomal and vacuolar membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YPT35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714997.1">
        <property type="nucleotide sequence ID" value="XM_018856774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HKK0"/>
    <dbReference type="GeneID" id="30029750"/>
    <dbReference type="OrthoDB" id="10254720at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07280">
        <property type="entry name" value="PX_YPT35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037917">
        <property type="entry name" value="Ypt35_PX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="PX" evidence="10">
        <location>
            <begin position="37"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00033728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="17321" checksum="065B75B8F3618BB6" modified="2016-10-05" version="1">MAPQKQATKLKHVAPAPIQLTEGVSSHDHVTDASHITDVMVGEWHLISGDGLSGLYVVWSIRVVIDNALHSLILLYKRYSDIEKLRNALTKAYKDDVFPVLPPKDTFSLLRAWQLESWLEHRRKGLQWFLTNTLLNPKYQHDPIITDFVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKK3</accession>
    <name>A0A1A0HKK3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exportin-5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_134721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715002.1">
        <property type="nucleotide sequence ID" value="XM_018854372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKK3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKK3"/>
    <dbReference type="GeneID" id="30027348"/>
    <dbReference type="OrthoDB" id="2215036at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042565">
        <property type="term" value="C:RNA nuclear export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045478">
        <property type="entry name" value="Exportin-5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF3">
        <property type="entry name" value="EXPORTIN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19273">
        <property type="entry name" value="Exportin-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exportin-5 C-terminal" evidence="2">
        <location>
            <begin position="351"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1201"/>
            <end position="1233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1233" mass="141979" checksum="40BEBAC4C7BAC600" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYW5</accession>
    <name>A0A1A0GYW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_28455</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000012">
        <property type="protein sequence ID" value="OBA16946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709248.1">
        <property type="nucleotide sequence ID" value="XM_018855356.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYW5"/>
    <dbReference type="STRING" id="869754.A0A1A0GYW5"/>
    <dbReference type="GeneID" id="30028332"/>
    <dbReference type="OrthoDB" id="10267969at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF50">
        <property type="entry name" value="VACUOLAR MEMBRANE PROTEIN YOR292C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="41"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA16946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="292" mass="33401" checksum="52015E59BF814C55" modified="2016-10-05" version="1" fragment="single">LINAIRIHNVPVLNMCIVAVLGIFLVKLTTKYKDLYMKNSLVTIIVTNLVLYGISETMAQSILSFSMQAPALSVSLFDTENLRNPAAVSVNSPVALGLQPRVPGGFQQGSGSVHEEMESDDSDVDDENLDRFLRLIRSDTSLPYQELLSDENVEQTYELSYFKFSRLAGFMCWGFGMAFVQCWWYRFLQIYSKQPQFIEVLRKVLSDQFCFLPVSLFCFFTYGTMVLESGTWDDARAKLRKLYLPTLVINYCVWFPVQFVNFLLVPRDFQVPFSSSISVLWNCFLSMRNSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYZ6</accession>
    <name>A0A1A0GYZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33876</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709275.1">
        <property type="nucleotide sequence ID" value="XM_018856415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYZ6"/>
    <dbReference type="STRING" id="869754.A0A1A0GYZ6"/>
    <dbReference type="GeneID" id="30029391"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA16976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="330" mass="36070" checksum="93AE145918ADA058" modified="2016-10-05" version="1" fragment="single">IFNIFLASNVWAMLIERNTTMSTMNELSFTSLTVRPEVDFSVINSNLFTVLGLFKNEGNINITSSSSRTTGVWITGGEFENLGNVILNSSSNDAFSEFHITLLGSFQNTGNISFGIQGGSYEIAPFSVTSVTEWYNTGIMVFAATYGVDVRLNFECRSSSNELTSIVNDGTVCLNNTLWPVKKTIEGIGCITLGSGGQLDLQYSQRTYALAAAQTVYLASFDSLLKVTGWGLFEGNIPVIKVAGFGNRNLIRLHTYSVNGFRYSLTTGMLTVLVGNIHEVNFDIGTDYIMPFFRLTSSAIYYIGNPPHSPPDICFCATTFHTAPRALGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ96</accession>
    <name>A0A1A0GZ96_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33845</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709312.1">
        <property type="nucleotide sequence ID" value="XM_018856406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ96"/>
    <dbReference type="GeneID" id="30029382"/>
    <dbReference type="OrthoDB" id="4096531at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="467" mass="52348" checksum="7827CC90A69FF8D5" modified="2016-10-05" version="1">MFKIISFSIILSVVALLWLVYHSPVKEFLLETSTQFRNLVVCSDTQAAQMCWKKFGPAILALAKSSFSNLAYQLVEVCLHETESQHLHDLQFMLNVQKCFHAADFIESRSGCCSAKSSVREVASVDLWLTFLKTLLLVYLVFNVPERAILKIREAFHASYEVSTSFWKSFLAVFSPFLKVLLTSDSLDGAANSLENVYEASPKMVPPGENMGAKWHNNVQTTLCTRHEACTEKKGLLRVVKKPHNACPRVVRGCREQSNSIPATSECARDASDTETHTMCGHLDEDDRCSEGRFEVTCSQSLATNTSGKLNLNKAFLSDGELSDSTRSSHRQKLLLGPHESTSADDIEFLVSLPPSSPKEFENRIQTGSLSNLLDKNILPGAYGRPSFNISTEAGRAKMRAYVKAKMMHKIPGSLEKHSYRQLSRVDSDNQRMRRVFVANKGWIWRTRLETHGTRFVTHNDTEAPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H270</accession>
    <name>A0A1A0H270_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA mismatch repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_47790</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. MSH3 provides substrate-binding and substrate specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer consisting of MSH2-MSH3 (MutS beta). Forms a ternary complex with MutL alpha (MLH1-PMS1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709417.1">
        <property type="nucleotide sequence ID" value="XM_018858222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H270"/>
    <dbReference type="STRING" id="869754.A0A1A0H270"/>
    <dbReference type="GeneID" id="30031198"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032137">
        <property type="term" value="F:guanine/thymine mispair binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032138">
        <property type="term" value="F:single base insertion or deletion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043570">
        <property type="term" value="P:maintenance of DNA repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043111">
        <property type="term" value="P:replication fork arrest"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1420.10:FF:000019">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.10:FF:000002">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000771">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003810">
        <property type="entry name" value="PRK05399.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 11 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="14">
        <location>
            <begin position="993"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="70"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA18022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1165" mass="131123" checksum="298595D9951D0017" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4M7</accession>
    <name>A0A1A0H4M7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein artemis</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA cross-link repair 1C protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_47546</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709526.1">
        <property type="nucleotide sequence ID" value="XM_018858187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4M7"/>
    <dbReference type="STRING" id="869754.A0A1A0H4M7"/>
    <dbReference type="GeneID" id="30031163"/>
    <dbReference type="OrthoDB" id="5561659at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="13">
        <location>
            <begin position="342"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA18991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="425" mass="48332" checksum="1C8BD2D01321A4EF" modified="2016-10-05" version="1" fragment="single">MSNTFDGVLREFPYIGVDRFNTSASVFLLTHCHADHLSGITNKSFTSIVYCSEETKLLLSLDSRLKGLLNLITGVPFNVKTRLETPHDTANAIYITLIEAYHCLGACMFLVEERVSRAVLCTGDIRAESWWRESARLSPALFPYTSGDKILNCIYFDSTFAYRGEPYIEIPTNFNGIHTAISMLREYPKNDPEIKFEFCDTTLGFEQAWAYVLSYFGGSLVVKASSLKQKLLLVAQYDRINGNILRRAMLPPTAFNTGPVFYGGSSPKGDFSIVRIKQCINFNIKDYTGICCPISIESIPSATDLNLLKVTKAGNKIYEFLGRHWVLPLKQNELLPLKIKLLFSRHSSYSEVREFLSMFKPQEVFPCVYSQEVWSSGFIMARIFGDICSRQEFNYDSMMATMYGLPSQEIRDRPVVTINRWNIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V0</accession>
    <name>A0A1A0H4V0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_61153</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
            <location evidence="1 11">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the KRR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709510.1">
        <property type="nucleotide sequence ID" value="XM_018858314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V0"/>
    <dbReference type="STRING" id="869754.A0A1A0H4V0"/>
    <dbReference type="GeneID" id="30031290"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 11" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="13">
        <location>
            <begin position="120"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="273"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="231"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="273"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="327"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA18975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="345" mass="40118" checksum="E18C283745AD5E3B" modified="2016-10-05" version="1">MPSTHNRDKPWDTEDIDKWKIEEFKPEHNASGQPFSEESSFMTLFPKYRENYLRTVWADVTSALEGHNLACQLDLVEGSMTVKTTRKTFDPAIILKARDLIKLLARSVPFPQAVKVLQDDIACDVIKIGSYVSNKDRFVKRRQRLVGPNGNTLKALELLTKCYILVQGNTVSAMGPYKGLKELRRVVEDCMKNVHPIYHIKELMIKQELAKNPELANEDWSRFLPNFKKKNVARKKAKETKKPKVYTPFPPAQQPRKIDFQIESGEYFLGKKEKQRKDLQEKRAKQEEVSEERKAERIKAFEAPEEATRENRLLASSAGEIKDKKEKKEKKEKKEKKEKKDKKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V6</accession>
    <name>A0A1A0H4V6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Eaf N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_223494</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709493.1">
        <property type="nucleotide sequence ID" value="XM_018855160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V6"/>
    <dbReference type="STRING" id="869754.A0A1A0H4V6"/>
    <dbReference type="GeneID" id="30028136"/>
    <dbReference type="OrthoDB" id="3998262at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019194">
        <property type="entry name" value="Tscrpt_elong_fac_Eaf_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09816">
        <property type="entry name" value="EAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription elongation factor Eaf N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA18958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="393" mass="43087" checksum="79EDB701F965DE11" modified="2016-10-05" version="1">MNIPDGEYDIDLLVLDSPQTADVPSLAIRYGFIPDSMDQSNLLTLFQENEVCILEAHLIERANLSELVPIIFEGVPQRQRPTQSGASSVPYSYFLSFLLAEKNGTGSLLNVQLRRLESTVRVNKSRNADKWRKSIRQWRNSPVAKAEKAAPELSLAVKQKSPHVLSVLLKRKPVNPPARKGTNGTSSSSRPSRSGPEPAVKRAAASKRPPAAKPDTEQDIISVSDFDNLESDEEDAFPIFDAPSTKRSTPPSTENSKEDPVARGEGCSGPREVELGHDNQTPGTSGEGLENNIHDHEKDDDIDMSDEFADLEDQLDEVLVDDHAKPNLIDSDSDDDSDTGAFAGTPIIIDVGEEKKTGISLKLQRVAPQSKPMSLRELYGAEKTDYLSSSEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H597</accession>
    <name>A0A1A0H597_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_89138</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709617.1">
        <property type="nucleotide sequence ID" value="XM_018859295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H597"/>
    <dbReference type="STRING" id="869754.A0A1A0H597"/>
    <dbReference type="GeneID" id="30032270"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15492">
        <property type="entry name" value="PHD_BRPF_JADE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000007">
        <property type="entry name" value="Bromodomain containing 1, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="8">
        <location>
            <begin position="157"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="9">
        <location>
            <begin position="211"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="678" mass="77413" checksum="AEB35FC00C2095A4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5E9</accession>
    <name>A0A1A0H5E9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription activator GCR1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_47072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709680.1">
        <property type="nucleotide sequence ID" value="XM_018858121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5E9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5E9"/>
    <dbReference type="GeneID" id="30031097"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052146">
        <property type="entry name" value="HOT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022210">
        <property type="entry name" value="TF_GCR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784:SF2">
        <property type="entry name" value="HIGH-OSMOLARITY-INDUCED TRANSCRIPTION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37784">
        <property type="entry name" value="PROTEIN MSN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12550">
        <property type="entry name" value="GCR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription activator GCR1-like" evidence="2">
        <location>
            <begin position="201"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="33918" checksum="133B61330F8F11F4" modified="2016-10-05" version="1">MLPFHESQLKIADAMAKVEHEVATVNQDMAYFRHVVDFQNLKIEKLTLLLLDLLHNKDVAPIVAQLQGLQASDPFNVDDGPDAVDDALPENVHEPVHDQVVSVGSSVGVGAVLLDSNMDPQLHNVAQAAHSQHSQHSQHQGQRQQGHAQLLEHNSPRPQQDRQGAREDSDRSMSVVDMPGLEVVNINGMNGRKKPKVTVDFLHNPMTVKEIYDEFTVGFRGQPPLREMDERYGRHEWRGDSRSKESKRFQRRKKLCDAIERGMQKYGKTAEEIQAFIEDFRGDKSLTWVMNGNLPSDLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5J7</accession>
    <name>A0A1A0H5J7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar transporter chaperone complex subunit 4</fullName>
            <ecNumber evidence="3">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polyphosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">SPX-dependent polyphosphate polymerase VTC subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Vacuolar membrane polyphosphate polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_33502</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19574"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the VTC4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709848.1">
        <property type="nucleotide sequence ID" value="XM_018856312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5J7"/>
    <dbReference type="STRING" id="869754.A0A1A0H5J7"/>
    <dbReference type="GeneID" id="30029288"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016237">
        <property type="term" value="P:microautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07751">
        <property type="entry name" value="PolyPPase_VTC4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.100.30:FF:000001">
        <property type="entry name" value="Vacuolar transporter chaperone 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="654"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="694"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="16">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA19316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="718" mass="83417" checksum="3DE0869C36535AD1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5K8</accession>
    <name>A0A1A0H5K8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33393</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709714.1">
        <property type="nucleotide sequence ID" value="XM_018856280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5K8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5K8"/>
    <dbReference type="GeneID" id="30029256"/>
    <dbReference type="OrthoDB" id="278329at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006111">
        <property type="term" value="P:regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="121" mass="14162" checksum="442AAE2C1FB9D1E7" modified="2016-10-05" version="1">MRPSLVRLVRQRRPERKTEPLLPPLALYRSILRAHIHKLPAELRYLGDEYVKAEFKAHKSTDNPLYIVGFLTQWQDYLRSLDGGSWAEGKLTLQQLENMSPEQVGQLYELMQETQRLGSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Q3</accession>
    <name>A0A1A0H5Q3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_79815</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709817.1">
        <property type="nucleotide sequence ID" value="XM_018859130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Q3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Q3"/>
    <dbReference type="GeneID" id="30032106"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF8">
        <property type="entry name" value="BRCA1-ASSOCIATED RING DOMAIN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="565"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="598"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="634"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="481"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="991"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="105"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="145"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="533"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="549"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1239" mass="139836" checksum="305577DF93FF2B41" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5R9</accession>
    <name>A0A1A0H5R9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial intermediate peptidase</fullName>
            <ecNumber evidence="4">3.4.24.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_79781</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.</text>
            <dbReference type="EC" id="3.4.24.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase M3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.59" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709784.1">
        <property type="nucleotide sequence ID" value="XM_018859124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5R9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5R9"/>
    <dbReference type="GeneID" id="30032100"/>
    <dbReference type="OrthoDB" id="17530at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06457">
        <property type="entry name" value="M3A_MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033851">
        <property type="entry name" value="M3A_MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF79">
        <property type="entry name" value="MITOCHONDRIAL INTERMEDIATE PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="14">
        <location>
            <begin position="268"/>
            <end position="760"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="765" mass="87377" checksum="6243137AC1166EB3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5W9</accession>
    <name>A0A1A0H5W9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Translation initiation factor eIF4e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_79828</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709834.1">
        <property type="nucleotide sequence ID" value="XM_018859134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5W9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5W9"/>
    <dbReference type="GeneID" id="30032110"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="377"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="395" mass="44077" checksum="ACFBB6B08E65C344" modified="2016-10-05" version="1">MSENLKRAESLFNRIMNPNKQQGSSEPGPQKYHKAASHNHHQSGDPELGRGNGRHSSNHSNAHRYSGRSSYKHPTALAVSTVDVPKRDPEQEAKAAVSALAENLHVLPYCWTIWHHSRSKTKVPAVADEGGSAAPDAAKAGAAAVDSYLQAATEIEFPCFGQPEKPVKPIASLEQMWMSLSALKKSHELVNGSELLVFKTGVNPVWEDPINSRGGRWVFRFSHRVNPNAASQESQEAIQRGRRRATLVWERLVLKTLTGCILLGRKEHHDALLSDIVGLVLSVRRDEEIISIWNSNSHVSKKSADDEDKKGSVAGFHARRVYCDAILRVIREVDAIMQGSDCVETQLSSSNERVNGVTFEYRLHSDNSHPYNPNERRRGKGYHHHHQKEEKDEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6C4</accession>
    <name>A0A1A0H6C4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_46243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CENP-X/MHF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710167.1">
        <property type="nucleotide sequence ID" value="XM_018858015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6C4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6C4"/>
    <dbReference type="GeneID" id="30030991"/>
    <dbReference type="OrthoDB" id="2500381at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071821">
        <property type="term" value="C:FANCM-MHF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22921">
        <property type="entry name" value="HFD_CENP-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018552">
        <property type="entry name" value="CENP-X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28680">
        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28680:SF1">
        <property type="entry name" value="CENTROMERE PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09415">
        <property type="entry name" value="CENP-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="62"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="12547" checksum="16BC3AA8D139CB7E" modified="2016-10-05" version="1">MVAPTTIARVFSEVSFSNASTRITSSAVALSAEYIRLFVREAILRANAQRLEEAAPTEVDGIDNVTAAAAPPADYGDGSDASDDEGVALPVPTQLPPATDSSDTLTTRHLAAVSGLLVVDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6D3</accession>
    <name>A0A1A0H6D3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">H/ACA ribonucleoprotein complex subunit NOP10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Nucleolar protein 10</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Nucleolar protein family A member 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">snoRNP protein NOP10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_79535</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710048.1">
        <property type="nucleotide sequence ID" value="XM_018859083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6D3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6D3"/>
    <dbReference type="GeneID" id="30032059"/>
    <dbReference type="OrthoDB" id="13807at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904874">
        <property type="term" value="P:positive regulation of telomerase RNA localization to Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.40:FF:000002">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13305">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="6852" checksum="7C3F4503A1D5354D" modified="2016-10-05" version="1">MHLMYTIGPDGKRVYTLAKTTADGEITKSAHPARFSPDDKYSRQRVTLKKRFGLLPTQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6F9</accession>
    <name>A0A1A0H6F9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminoglycoside phosphotransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710020.1">
        <property type="nucleotide sequence ID" value="XM_018858013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6F9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6F9"/>
    <dbReference type="GeneID" id="30030989"/>
    <dbReference type="OrthoDB" id="191037at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052898">
        <property type="entry name" value="ACAD10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829:SF1">
        <property type="entry name" value="HAD FAMILY PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47829">
        <property type="entry name" value="HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoglycoside phosphotransferase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="399" mass="44989" checksum="48FE15C881362A93" modified="2016-10-05" version="1">MSEFTEIRNPIDVQALSAYLANVPASAIRGLDVPPFFGPFDIKQFTFGQSNPTYLVVDGSGSKYVLRRKPMPNSKLVSKSAHAIEREFFLLKGIENCNQRADPGKKVPVPKVYFLCEDESVLGVVFYVMEYVQGRSFKEPDMREVPAARQSEYWDSIMKTISAIHSVDCSELVKQLPAKHFPQFQPDQLERANKNGATYFQRQIRTLGAVEKSQSKTVDSIPGFSRLTQWVLDHSPRDPDHMTLVHGDCKIDNFLFHGEKPEVAAVLDWELCTMGHPIFDLANFLQPYTFPKSLNKAILQTASIGIEDPTSAQHVQTVLSLYEKFLGHKWRENDPRNNPHDLWKLGTVFGLLRLCVISQGVAMRVKRGTASSASAKAVAELYKTLSELALSFIEEDYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6I9</accession>
    <name>A0A1A0H6I9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_46229</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710050.1">
        <property type="nucleotide sequence ID" value="XM_018858012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6I9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6I9"/>
    <dbReference type="GeneID" id="30030988"/>
    <dbReference type="OrthoDB" id="2341546at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001278">
        <property type="term" value="P:positive regulation of L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF21">
        <property type="entry name" value="REGULATORY PROTEIN LEU3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="7">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="19"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="646"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="648"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="676"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="782" mass="86302" checksum="F1D7605186DEC86F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6X9</accession>
    <name>A0A1A0H6X9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Calcineurin regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein phosphatase 2B regulatory subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_79091</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Composed of a catalytic subunit (A) and a regulatory subunit (B).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710235.1">
        <property type="nucleotide sequence ID" value="XM_018858998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6X9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6X9"/>
    <dbReference type="GeneID" id="30031974"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000001">
        <property type="entry name" value="Calmodulin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="174" mass="19478" checksum="CD46D710BCCAD657" modified="2016-10-05" version="1">MGLASSKILDTLMEGTNFDREEIDRLRKRFMKLDRDRSGSIDKNEFLDIPGISSNPLATRLMDVFDLDRDGTIDFQEFITGLSAFSGKSSKTEKLQFAFKIYDIDRDGFISNGELFVVMKTMVGKNLLDAELQQVVDKTIMGADKDGDGKLSFEEFRLAVDLTLVASALTLLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Z3</accession>
    <name>A0A1A0H6Z3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_33307</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710201.1">
        <property type="nucleotide sequence ID" value="XM_018856266.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H6Z3"/>
    <dbReference type="GeneID" id="30029242"/>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23827">
        <property type="entry name" value="RWD_YLR419W-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF267">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE YLR419W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RWD" evidence="6">
        <location>
            <begin position="416"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="603"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="827"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1425" mass="160776" checksum="095F1C61195FE7A0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Z8</accession>
    <name>A0A1A0H6Z8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Regulator of rDNA transcription 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RRT14</name>
        <name evidence="10" type="ORF">METBIDRAFT_45070</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Involved in ribosome biogenesis, probably through modulation of rDNA transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
            <location evidence="2 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the RRT14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710399.1">
        <property type="nucleotide sequence ID" value="XM_018857873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Z8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Z8"/>
    <dbReference type="GeneID" id="30030849"/>
    <dbReference type="OrthoDB" id="4069371at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031404">
        <property type="entry name" value="Rrt14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17075">
        <property type="entry name" value="RRT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="194" mass="22274" checksum="FCC69830987AAC0A" modified="2016-10-05" version="1">MAFSSDLSKTRSQATLNKLFENMLPGSTTRSNIPLKKISTTENFSREVSKKRLSKEEIKKANKIEKAKRNKQLNKNLEKEKLFSKNVKYNVIKSHKNSQNISEEEQKYLKKLIKKNSFAVRRAGGLDDPMIKDEVEELRSEILALTNEKYDRSKERQQKAKLSSFNEKVKSGVLTYPGLTPGLAPVDYDESDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H718</accession>
    <name>A0A1A0H718_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_22042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710221.1">
        <property type="nucleotide sequence ID" value="XM_018855134.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H718"/>
    <dbReference type="GeneID" id="30028110"/>
    <dbReference type="OrthoDB" id="4085733at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="58" mass="6365" checksum="4FDC4202165D4614" modified="2016-10-05" version="1" fragment="single">ENVYLPPASSKKSPEVPSYHPCLIVQDSQTDLAKVKPCGCADAYCVKNAPLWIPRAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H754</accession>
    <name>A0A1A0H754_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HAD-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_79107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710258.1">
        <property type="nucleotide sequence ID" value="XM_018859000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H754"/>
    <dbReference type="STRING" id="869754.A0A1A0H754"/>
    <dbReference type="GeneID" id="30031976"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050849">
        <property type="entry name" value="HAD-like_hydrolase_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181:SF1">
        <property type="entry name" value="COLD TOLERANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28181">
        <property type="entry name" value="UPF0655 PROTEIN YCR015C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12710">
        <property type="entry name" value="HAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="30247" checksum="880E187A04462207" modified="2016-10-05" version="1">MSGRIFITDWDETATMADTTALIALAASRRDRDAVPPFTHFSQIYLDAYLAYIAQEASGCGRRDSVIAEEKFQQGMRAVEYTSIRALEETGYFKGFPIGKLRDLVPKVELRPGFVDFVKSAGCPVYVLSVNWCRRLIEWVLEFHGVRHVTVLANDFEVDAEDITTGKFDRRCDIRTGYDKAMELARIRSLHPALNLFYFGDSSGDVLPILKADYGGVITGGRARTVLGLLAEVYMLESDFRSPDSVGLYSQVPKVFEATWHELAAAWHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7A5</accession>
    <name>A0A1A0H7A5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_45560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710308.1">
        <property type="nucleotide sequence ID" value="XM_018857930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7A5"/>
    <dbReference type="GeneID" id="30030906"/>
    <dbReference type="OrthoDB" id="4023279at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="237" mass="27443" checksum="EFCAB86FDC81ED97" modified="2016-10-05" version="1">MASDNCYYNLNTENCIAMNQIAQEIYRSVRENDIILARLTDLVDTRLKLPNLADKSDNAEAEYSGDYLRSHLDNNLHLDSVEYDETKFAQVKSTRIRQLLIDNYRLLIIKQSKQAENKQLLSIYNSYEQILSETIVPKLTQDVSSLNIERIFAARILCAEHKFPLEDELWGTYCKYLESLESVRVFSNKLIEILVGHLHDEQMERLATQVNILGNLIKQLNGGGLHRNQISNFDYLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7N5</accession>
    <name>A0A1A0H7N5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal RNA-processing protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33066</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710634.1">
        <property type="nucleotide sequence ID" value="XM_018856210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7N5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7N5"/>
    <dbReference type="GeneID" id="30029186"/>
    <dbReference type="OrthoDB" id="551633at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000477">
        <property type="term" value="P:generation of mature 5'-end of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="97"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="227"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="245"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="257" mass="30002" checksum="28682E0B2190816F" modified="2016-10-05" version="1">MANKKPSPRTNRDILTGGSKYRSKQTKKFGVEEVVFDKDSRHEFLTGFHKRKVERQKKAKKFHEEQDRLAKIEERKQIREEKQKEFEAKMEELKEAKNLDVSEILKQENDEEQWEGFGSDDQDNEEKPSDIASDDEPLKGILQKKQIYKIDNLEALGDAVVDDETTVTVEALENPNFARVGQSSLDLMAQTNNVNLSKSEEVLEKSIERAKKYAVVCGVSKPQQKQKKKKFRYLTKTERRENNRKVKLNKLKSRKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Q9</accession>
    <name>A0A1A0H7Q9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_45021</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Required for vacuolar assembly and vacuolar traffic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the VPS41 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710659.1">
        <property type="nucleotide sequence ID" value="XM_018857860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Q9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Q9"/>
    <dbReference type="GeneID" id="30030836"/>
    <dbReference type="OrthoDB" id="244107at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030897">
        <property type="term" value="C:HOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032258">
        <property type="term" value="P:cytoplasm to vacuole targeting by the Cvt pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035542">
        <property type="term" value="P:regulation of SNARE complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006624">
        <property type="term" value="P:vacuolar protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042144">
        <property type="term" value="P:vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099022">
        <property type="term" value="P:vesicle tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000933">
        <property type="entry name" value="Vacuolar protein sorting-associated protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000547">
        <property type="entry name" value="Clathrin_H-chain/VPS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016902">
        <property type="entry name" value="Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045111">
        <property type="entry name" value="Vps41/Vps8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12616:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 41 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23556">
        <property type="entry name" value="TPR_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028921">
        <property type="entry name" value="VPS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00299">
        <property type="entry name" value="CLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50236">
        <property type="entry name" value="CHCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Vps41 beta-propeller" evidence="7">
        <location>
            <begin position="41"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="repeat" description="CHCR" evidence="5">
        <location>
            <begin position="636"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR028921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="935" mass="107339" checksum="668DDF161A57FBB1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7V8</accession>
    <name>A0A1A0H7V8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 18">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 18">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_79293</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18 19">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="5 18"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710502.1">
        <property type="nucleotide sequence ID" value="XM_018859033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7V8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7V8"/>
    <dbReference type="GeneID" id="30032009"/>
    <dbReference type="OrthoDB" id="448573at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00615"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035865">
        <property type="term" value="P:cellular response to potassium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000022">
        <property type="entry name" value="CDP-diacylglycerol--serine O-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016271">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase_fun"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF61">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000852">
        <property type="entry name" value="Phosphatidylserine_synth_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 18 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 18" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRNR" id="PIRNR000852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA19977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="268" mass="29874" checksum="6F22B82710D2FAE1" modified="2016-10-05" version="1">MSDSYSSGYRKNENAIASDAESDAEIIAPHPPIRRSSSLFSLSSNQEPLPRPEPKDYQHFLGDSRHFSLIRNLHMADFITLLNGFSGFYSIISCLRFALSGQTHYIQRAHFFICLGLFFDFFDGRVARLRNKSSLMGQELDSLADLISFSVSPAIIAFSIGFRSTVDVLILSYWILCGLLRLARFNISSNNIPKDSHGKSQYFEGLPVPSNLLWVAIMSYLVHKDMILANLPGGLIFEGTVLELHAVSLAFILQGSAEISKSLHIPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Y2</accession>
    <name>A0A1A0H7Y2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ino eighty subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_16572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710527.1">
        <property type="nucleotide sequence ID" value="XM_018854659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Y2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Y2"/>
    <dbReference type="GeneID" id="30027635"/>
    <dbReference type="OrthoDB" id="5413003at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038014">
        <property type="entry name" value="Ies1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287:SF1">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="614" mass="70989" checksum="CB2981BBA29C866E" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H861</accession>
    <name>A0A1A0H861_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_12332</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710816.1">
        <property type="nucleotide sequence ID" value="XM_018854213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H861"/>
    <dbReference type="GeneID" id="30027189"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="162" mass="17723" checksum="B710ED3005B9F84E" modified="2016-10-05" version="1">MQRSFTTSGFSGLLPRNNGHYIRAVSALTVAPFRVPSYYLLAHFQAYSLATQHPWVAVCGLTASDPPNQFAGLALSAVILAKAAFPAEHSYYATRDDAFTVVQTHDPCPVNSQTQTLIKAPTYACIRIPHTSRYMRLDEYSSQTLALKMSPGARNARVVSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8J4</accession>
    <name>A0A1A0H8J4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein serine/threonine phosphatase 2C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_17030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the PP2C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710729.1">
        <property type="nucleotide sequence ID" value="XM_018854684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8J4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8J4"/>
    <dbReference type="GeneID" id="30027660"/>
    <dbReference type="OrthoDB" id="10264738at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000222">
        <property type="entry name" value="PP2C_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01032">
        <property type="entry name" value="PPM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="5">
        <location>
            <begin position="23"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="36734" checksum="FA42C68E3CC4195D" modified="2016-10-05" version="1" fragment="single">MGQLMSHPMEEKSIEYHTFDTISYCVGAMQGYRLSMEDAHDARISENEALAVFGVFDGHGGKDVAHILRSTLVAKIFKHLNGYITAGSAPPRLATFTKTLTNCFFEVDAGLPDKTAANCGSTAIVLTVVAGRYIIVANTGDSRSILSLPGGHAKTLSFDHKPSHMGERVRIENSGGYVLNSRVNEILALSRAFGDLKFKIPWLQLDAVGKNNSVLEQNRKYCKQDLVLLPPELFQVTVEPELMVYDLQALDMPEFMVLACDGIWDCFTNDQLVRAIREKLGHGWTLQHITEYVLNECIAMASSATGIGFDNMTLMIVALHPKTTMDEWRTAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8J9</accession>
    <name>A0A1A0H8J9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein L24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_32358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710868.1">
        <property type="nucleotide sequence ID" value="XM_018856037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8J9"/>
    <dbReference type="STRING" id="869754.A0A1A0H8J9"/>
    <dbReference type="GeneID" id="30029013"/>
    <dbReference type="OrthoDB" id="1688503at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="180" mass="19982" checksum="E2FC315EBB66448D" modified="2016-10-05" version="1">MNPCCFSRTIKLDSPIKSRLFAKSAYIPPCLSKPTLPNTIAWVSSISAPVPKTTILTIPDVSSSRSKSRKAHFSSSSAERRVLMSAPLSKELRKQYNIKSIPIRKEDEVTVVRGSKKGSEGKISTVYRLKFSVQIEKLQIEKSSGASIPINIHPSKVVITKLYLDDKRKALIERKGGKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Q3</accession>
    <name>A0A1A0H8Q3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_78777</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710897.1">
        <property type="nucleotide sequence ID" value="XM_018858937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Q3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8Q3"/>
    <dbReference type="GeneID" id="30031913"/>
    <dbReference type="OrthoDB" id="2537459at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291696" description="WSC domain-containing protein" evidence="3">
        <location>
            <begin position="17"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="16"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="351"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="456" mass="46610" checksum="58E3D7AD342C0E91" modified="2016-10-05" version="1">MAYMLLLLAYMDLAAALAITLCSSTNLGSGQTSDSYMSNGYCRDYCADAGYSVAITQNYDCWCSNTVPADVVDDSQCDIGCPGYSEEEVCGGDGVYGYVVFGSASTATRSDQSTASTATRSDQSTSSSKPATTRSSSSSSSSSSSSSSSSSSSSSSSSTSTPSTSQPAPPTSSTSSETSATTDSVAQVASSPIVSALPSSESSSSPASPSASPSSSASTSASSPPVSSDIAMVTRATTIVSVVTIDGTLTQLVLTKYVTATASTSASASLLSAAALGNSFFDSTAKVAGTFTAVGVVAAALVAALLYCCCFAARTHRDDYTDEEKQYTSDDTSVTNAAPVGTPGGPSPKTRSSSHSSLRRSSISKPIMSMFNAVSGGTGGIASATLGRSLSRKNLSPKNEVQDTGGPIMAPILEFDNVLEPHAMFEEYNNSMMSLNDENDYSRRIWHVTNPEHSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8T5</accession>
    <name>A0A1A0H8T5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NADH-quinone oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_42962</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710932.1">
        <property type="nucleotide sequence ID" value="XM_018857602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8T5"/>
    <dbReference type="STRING" id="869754.A0A1A0H8T5"/>
    <dbReference type="GeneID" id="30030578"/>
    <dbReference type="OrthoDB" id="268400at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12280:FF:000001">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit B 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11995:SF14">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="4">
        <location>
            <begin position="62"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="187" mass="20832" checksum="82E27969BEF470CE" modified="2016-10-05" version="1">MASAKVGLPARDLTPKPMATDFPLMSQKTASAPIDYALTSLDAIAMWARKSSFWPVTFGLACCAVEMMHVSAPRYDQDRLGIIFRASPRQSDIMIVAGTLTNKMAPALRQVYDQMADPRWVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALMYGVFQLQKKMMKTRVTRLWYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8X3</accession>
    <name>A0A1A0H8X3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell wall mannoprotein PIR1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_24197</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PIR protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710972.1">
        <property type="nucleotide sequence ID" value="XM_018855276.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8X3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8X3"/>
    <dbReference type="GeneID" id="30028252"/>
    <dbReference type="OrthoDB" id="5415592at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005199">
        <property type="term" value="F:structural constituent of cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054508">
        <property type="entry name" value="PIR1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051153">
        <property type="entry name" value="Yeast_CWMannoprotein_PIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000420">
        <property type="entry name" value="Yeast_PIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47254:SF1">
        <property type="entry name" value="CELL WALL MANNOPROTEIN CIS3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00399">
        <property type="entry name" value="PIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22799">
        <property type="entry name" value="PIR1-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00929">
        <property type="entry name" value="PIR_REPEAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50256">
        <property type="entry name" value="PIR_REPEAT_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Cell wall mannoprotein PIR1-like C-terminal" evidence="8">
        <location>
            <begin position="218"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="302" mass="31391" checksum="5F7E225A33C1B9B4" modified="2016-10-05" version="1" fragment="single">ILSAIALAQAAYLPSEPWTTLTPSATYPGATTDHFHSFGIQVVSITTASSEAASSSTAAVAKRGVINQIGDGQIQNQSSETLSTPTITTASVINQISDGQIQAQTTTTSSTSTSSSKITTASVINQISDGQIQDQTTTSTTSSSSSMKTTASVINQISDGQIQDQTTSASTSTNTAAVASQISDGQVQATDSVFKEETDGFSVSCSTSNSLMMVLQNGELKDSNGRIGAIVANRQFQFDGPPPQAGTIYAAGWSVTEDHYLALGDSDVFYLCVSGDFSNLYDENVAAQCSPVHLEVINFVSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H906</accession>
    <name>A0A1A0H906_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peroxisomal biogenesis factor 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_32487</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711007.1">
        <property type="nucleotide sequence ID" value="XM_018856057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H906"/>
    <dbReference type="STRING" id="869754.A0A1A0H906"/>
    <dbReference type="GeneID" id="30029033"/>
    <dbReference type="OrthoDB" id="411017at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001579">
        <property type="term" value="P:medium-chain fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044375">
        <property type="term" value="P:regulation of peroxisome size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008733">
        <property type="entry name" value="PEX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652:SF50">
        <property type="entry name" value="PEROXIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12652">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05648">
        <property type="entry name" value="PEX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="1" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00046271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="228" mass="25763" checksum="AE16C6795A53C422" modified="2016-10-05" version="1">MVADCIIYHPTLTKLLKFIDSTPKREKSFRLVAYLSRFLGYYLQRRGYSAELVRKFADLKQHATLIRKGMRFLKPLNHLQAASKAYDNKLMDLVLLLTTVARNLGYAGYLALDQVTFFKMLGLVDKRKYTWVPVWAARCWLAGLVAGVVHSLRTYQINRAKMAAVEEAGDTCALQGKVYAAKRKLVWDALDMFVCLNTLNYLHFSEGDVGLAGAVTSLMGLKDLWAAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9E6</accession>
    <name>A0A1A0H9E6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cyclin-dependent kinase 1</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Cell division control protein 28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_32494</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711020.1">
        <property type="nucleotide sequence ID" value="XM_018856060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9E6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9E6"/>
    <dbReference type="GeneID" id="30029036"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990758">
        <property type="term" value="P:mitotic sister chromatid biorientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001033">
        <property type="term" value="P:negative regulation of double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051447">
        <property type="term" value="P:negative regulation of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903500">
        <property type="term" value="P:negative regulation of mitotic actomyosin contractile ring assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045930">
        <property type="term" value="P:negative regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045875">
        <property type="term" value="P:negative regulation of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905168">
        <property type="term" value="P:positive regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045819">
        <property type="term" value="P:positive regulation of glycogen catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051446">
        <property type="term" value="P:positive regulation of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045931">
        <property type="term" value="P:positive regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904291">
        <property type="term" value="P:positive regulation of mitotic DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010696">
        <property type="term" value="P:positive regulation of mitotic spindle pole body separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901319">
        <property type="term" value="P:positive regulation of trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010898">
        <property type="term" value="P:positive regulation of triglyceride catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990139">
        <property type="term" value="P:protein localization to nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902889">
        <property type="term" value="P:protein localization to spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010568">
        <property type="term" value="P:regulation of budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010570">
        <property type="term" value="P:regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905634">
        <property type="term" value="P:regulation of protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090169">
        <property type="term" value="P:regulation of spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032210">
        <property type="term" value="P:regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07835">
        <property type="entry name" value="STKc_CDK1_CdkB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000144">
        <property type="entry name" value="Cyclin-dependent kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000027">
        <property type="entry name" value="Putative Cyclin-dependent kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 19" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 17" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="7"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA20498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="310" mass="36028" checksum="FC0CB8C835E5FE05" modified="2016-10-05" version="1">MVELSDFQRQEKVGEGTYGVVYKALDTRYNNRVVALKKIRLESEDEGVPSTAIREISLLKEMRDDNIVRLYDIIHSDSHKLYLVFEFLDLDLKKYMESIPQGVGLEPSMVKRFMHQLVKGIKYCHSHRVLHRDLKPQNVLIDKEGNLKLADFGLARAYGVPLRAYTHEVVTLWYRAPEILLGGKQYSTGVDMWSVGCIFAEMCNRKPLFPGDSEIDEIFRIFRILGTPNEEIWPDVSFLPDFKKTWPKWEKKDLAKSVPTLDLDGVNLMQQMLNYNPSNRISAKRALIHPYFQEDTEENYDNYLRSVTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9E8</accession>
    <name>A0A1A0H9E8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32506</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0357 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711034.1">
        <property type="nucleotide sequence ID" value="XM_018856063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9E8"/>
    <dbReference type="GeneID" id="30029039"/>
    <dbReference type="OrthoDB" id="447314at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018559">
        <property type="entry name" value="DUF2015"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28023:SF1">
        <property type="entry name" value="UPF0357 PROTEIN YCL012C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09435">
        <property type="entry name" value="DUF2015"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="99" mass="11557" checksum="B5BFD8953CBFCB67" modified="2016-10-05" version="1">MFEAYRNRQRWQRLPHGPEFGFQDDMENGLSSNQFDLNKNIVNNDTRTLDDAAKGEIRRMMSQENITFDEARLKYFQCKLSEHGIGADGVPRDPKAVMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9M3</accession>
    <name>A0A1A0H9M3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ras-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_71885</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711212.1">
        <property type="nucleotide sequence ID" value="XM_018858401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9M3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9M3"/>
    <dbReference type="GeneID" id="30031377"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032889">
        <property type="term" value="P:regulation of vacuole fusion, non-autophagic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981">
        <property type="entry name" value="RAB FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47981:SF20">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB-7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0636">Prenylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="73"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="325" mass="35788" checksum="94618D0CBC15BD28" modified="2016-10-05" version="1">MLNGRPQKEPDIKETPSEPKSRQPSQAASPTLKVVLLGDLGVGKTCLRSQFVHHVFSNAYKATIGGDYLTTTVPVGPEAGEPGKTSDPGEISDRGESGGLSDTGETSDPGELSDPGAPRAVSLQVWDTAGQERYNLISQAFYRGADVAVLVYDVTSYESLLSVRDWFARFMQHCQVESPGIVIVGNKLDRAGDRAVDRLEVQDVLCRNAAVPLAEHVGCWAEDVMEISARTLASVDAVFRRVAVVGLAKMHDRHHHRCAVGLHEICELYGLYELCGLDELYGLDELHEICELYGLYELHGFYELSGLHELYGLYELYGLYEFYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9T9</accession>
    <name>A0A1A0H9T9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Peptidase S59 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_166196</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711404.1">
        <property type="nucleotide sequence ID" value="XM_018854663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9T9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9T9"/>
    <dbReference type="GeneID" id="30027639"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044613">
        <property type="term" value="C:nuclear pore central transport channel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2360:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1610.10:FF:000003">
        <property type="entry name" value="Nucleoporin SONB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025574">
        <property type="entry name" value="Nucleoporin_FG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13634">
        <property type="entry name" value="Nucleoporin_FG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="10" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="13">
        <location>
            <begin position="883"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="493"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="632"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="673"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="735"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="779"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="638"/>
            <end position="648"/>
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            <end position="688"/>
        </location>
    </feature>
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        <location>
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            <end position="717"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="OBA20894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1027" mass="100319" checksum="C97941CD219E1138" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9W7</accession>
    <name>A0A1A0H9W7_9ASCO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">METBIDRAFT_11528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
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            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711434.1">
        <property type="nucleotide sequence ID" value="XM_018854013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9W7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9W7"/>
    <dbReference type="GeneID" id="30026989"/>
    <dbReference type="OrthoDB" id="5563016at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="355"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="643"/>
        </location>
    </feature>
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            <end position="831"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="45"/>
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            <end position="155"/>
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    </evidence>
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            <dbReference type="EMBL" id="OBA20924.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1277" mass="139684" checksum="B44741CE1EE340F8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Z9</accession>
    <name>A0A1A0H9Z9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12654</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018854250.1"/>
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    <dbReference type="STRING" id="869754.A0A1A0H9Z9"/>
    <dbReference type="GeneID" id="30027226"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045211">
        <property type="entry name" value="TFP11/STIP/Ntr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329:SF1">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="681" mass="77468" checksum="24AF7FE05249CED0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA32</accession>
    <name>A0A1A0HA32_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_79019</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
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                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
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                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018858986.1"/>
    </dbReference>
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    <dbReference type="STRING" id="869754.A0A1A0HA32"/>
    <dbReference type="GeneID" id="30031962"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033698">
        <property type="term" value="C:Rpd3L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070210">
        <property type="term" value="C:Rpd3L-Expanded complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061188">
        <property type="term" value="P:negative regulation of rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061186">
        <property type="term" value="P:negative regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016479">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000219">
        <property type="term" value="P:positive regulation of invasive growth in response to glucose limitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17016">
        <property type="entry name" value="ING_Pho23p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="279"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="177"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="177"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="251"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="37647" checksum="B733079281078378" modified="2016-10-05" version="1">MHSDLPARPKSQRLANRTKADKPLQYFQQFQKRAQNITNINDLLPGLNDISDAFEAMPMDLFKYFTLLKEIDAKCIKTVPLINHNIGQYIDQLHTKPEAAAPHAHMTRLQRIRKQIHEVIPCLEEKMHVTSVAADVMAKHMYRINNDYKVIIGNNEIPESVRVGPLNHRALILDPQAAADAAKSAQSQRSETRREALAARKAHKDPEDEDGSKKRKTKEPTPLEPAASSSGNVKKRRKDKEPEKAVAPVAADKKKTGAKPRKDREPEPASHPGSAGAEPTYCYCNQISFGEMVGCDGDTCEREWFHLPCIGFKNPPKGKWYCDDCVAKMKKAKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA97</accession>
    <name>A0A1A0HA97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Dynein light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_32755</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Cytoplasmic dynein consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits which present intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
            <location evidence="1 15">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the dynein light chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711320.1">
        <property type="nucleotide sequence ID" value="XM_018856123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA97"/>
    <dbReference type="STRING" id="869754.A0A1A0HA97"/>
    <dbReference type="GeneID" id="30029099"/>
    <dbReference type="OrthoDB" id="10033309at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.740.10:FF:000005">
        <property type="entry name" value="Dynein light chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019763">
        <property type="entry name" value="Dynein_light_1/2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11886:SF35">
        <property type="entry name" value="DYNEIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01239">
        <property type="entry name" value="DYNEIN_LIGHT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 15" id="KW-0243">Dynein</keyword>
    <keyword evidence="6 15" id="KW-0493">Microtubule</keyword>
    <keyword evidence="12 15" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="11" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 15" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="65" mass="7555" checksum="C4BC582CCCCD7A61" modified="2016-10-05" version="1">MAVEALRTYQIEREIATYLKKELDLLYGASWHVIVGKSFGSHVTHEQGYFAYFYIGEMAFLVFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAD6</accession>
    <name>A0A1A0HAD6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 4E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_170173</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711486.1">
        <property type="nucleotide sequence ID" value="XM_018854680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAD6"/>
    <dbReference type="STRING" id="869754.A0A1A0HAD6"/>
    <dbReference type="GeneID" id="30027656"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098808">
        <property type="term" value="F:mRNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901195">
        <property type="term" value="P:positive regulation of formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019770">
        <property type="entry name" value="TIF_eIF_4E_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00813">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="208" mass="24047" checksum="FA078DE70F86D552" modified="2016-10-05" version="1">MSEDLTKKTEDLTLENPTTVLSNKKDFNSKHPLNSKWTLWYTKPQVNKSETWADLLKPVITFDTVEEFWGIHNSIPVANQLPIKSDYHLFKEGIKPEWEDEKNAKGGRWQYAFVNKREANPVINDLWLRGLLAVIGETIEDDENEVNGIVLNIRRGYRVAIWTKDCDSAKLKTIGERFKKVLKLADDQKLEFMSHDTFNAKGEPQLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB66</accession>
    <name>A0A1A0HB66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Growth regulation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_178465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711740.1">
        <property type="nucleotide sequence ID" value="XM_018854737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB66"/>
    <dbReference type="STRING" id="869754.A0A1A0HB66"/>
    <dbReference type="GeneID" id="30027713"/>
    <dbReference type="OrthoDB" id="9451547at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903293">
        <property type="term" value="C:phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903452">
        <property type="term" value="P:positive regulation of G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13384:SF16">
        <property type="entry name" value="GROWTH REGULATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="267"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="38846" checksum="2A7B103E66BA27A5" modified="2016-10-05" version="1">MAENLVTQVTQDVGGDAAAALTHEEENMNIDYNSKIHLNIRGKAFSITRDELMSLPESILLSLFPNGVFLDFNGQVISKLTEEDVVFVNFDPASFEYILRTFGDAHADLQGMTNLSPQISENSHLDSSPSVLQTKPAIIVLREDLDYYVIPPSAGCSSEDIKHLKLEVANKLMENKMIFSGLGYDTSLPSSITQKLGPAEQHLLDMLCSSGFERRESWGSRSLEPSKCVLQSLLLIRLANPLVTPPDSPSLNPVSSHASTRSRSRSRIATLASSASRAASRSMSRARKNTEINHSKLLLFWKKPARKCWWSNEEIEVQVAATGLSKLGDNVKVKVHIRRVWTLELSVIGVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB71</accession>
    <name>A0A1A0HB71_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_31806</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711745.1">
        <property type="nucleotide sequence ID" value="XM_018855923.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HB71"/>
    <dbReference type="GeneID" id="30028899"/>
    <dbReference type="OrthoDB" id="341578at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004694">
        <property type="term" value="F:eukaryotic translation initiation factor 2alpha kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071232">
        <property type="term" value="P:cellular response to histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071264">
        <property type="term" value="P:positive regulation of translational initiation in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990611">
        <property type="term" value="P:regulation of cytoplasmic translational initiation in response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23823">
        <property type="entry name" value="RWD_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14046">
        <property type="entry name" value="STKc_EIF2AK4_GCN2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016255">
        <property type="entry name" value="Gcn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024435">
        <property type="entry name" value="HisRS-related_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF160">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12745">
        <property type="entry name" value="HGTP_anticodon2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000660">
        <property type="entry name" value="Ser/Thr_PK_GCN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00591">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50908">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 12 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RWD" evidence="17">
        <location>
            <begin position="10"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="235"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="623"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="702"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="766"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1534"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="115"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="774"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="797"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1537"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11">
        <location>
            <position position="869"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="629"/>
            <end position="637"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="652"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="653"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000660-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1692" mass="191762" checksum="84E00B58CEEC7223" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBB0</accession>
    <name>A0A1A0HBB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aminodeoxychorismate synthase</fullName>
            <ecNumber evidence="4">2.6.1.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Para-aminobenzoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">p-aminobenzoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_78387</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate + L-glutamine = 4-amino-4-deoxychorismate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11672"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58406"/>
            <dbReference type="EC" id="2.6.1.85"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 4-aminobenzoate from chorismate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.85" evidence="4"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711929.1">
        <property type="nucleotide sequence ID" value="XM_018858875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBB0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBB0"/>
    <dbReference type="GeneID" id="30031851"/>
    <dbReference type="OrthoDB" id="64220at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00149"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008153">
        <property type="term" value="P:4-aminobenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010117">
        <property type="entry name" value="PabB_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01823">
        <property type="entry name" value="PabB-fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF18">
        <property type="entry name" value="AMINODEOXYCHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="3"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="12">
        <location>
            <begin position="283"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="11">
        <location>
            <begin position="489"/>
            <end position="766"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="775" mass="86170" checksum="525842BEF8FE74D4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBJ3</accession>
    <name>A0A1A0HBJ3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_182167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711891.1">
        <property type="nucleotide sequence ID" value="XM_018854782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBJ3"/>
    <dbReference type="GeneID" id="30027758"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="198" mass="22293" checksum="794CE56A406A449C" modified="2016-10-05" version="1">MPIFRAFRDQGPNWHPSWCCRASRLPAQATHTQQRAPGLMKGPPKSAAASLFLALLWPAGRQWRPNCVPSLKKKKKNPRGQNWRSLLSCWRSRPMPSRTAVANVRIGPCENCPMKSRPEGARARCAGFREKTRIRGKKIHGSPEPVFSAIPKNGPYLQNCHQSRPPSAFCCPLSCQHTPQHSSLLSREHRKTLSFLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBV0</accession>
    <name>A0A1A0HBV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HRQ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712124.1">
        <property type="nucleotide sequence ID" value="XM_018857445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBV0"/>
    <dbReference type="STRING" id="869754.A0A1A0HBV0"/>
    <dbReference type="GeneID" id="30030421"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021848">
        <property type="entry name" value="HODM_asu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11927">
        <property type="entry name" value="HODM_asu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="53382" checksum="C6C5A85D0A1C61C1" modified="2016-10-05" version="1">MEETFINATLNWESTLVLFPDSLYIPLGAVILLLTALSSLAPQYFHKKIGQTAKPEGKKENSKLPQGMPTPTPLMITPDQVKNYDDRPWRPFRWPYHQTMSIYKLDVNHWLDMDKYYWHYIEEKKRIWHQYGKENIDWLPEAYDACQELMETVADHMVARYPLLFTVLVDKKENGKIVRNEMTKEILDMTLPLKEHPLLFVTKMAKEDFYVVLKNPKDGMHYLVAAAVPFPGGSFGIDLKIGKHLDVIHDDVPYYQEKLKKSMERWFERLEPSSSVERASWYITWDHKLRVNNIYQLPKFVPNLEEDMKSTDPKEFNVRVERQTLRRLPKSQAIIFTNHPIFYSIEEMKDEPNVPSLLKKIIFEAPEKIIKYKNFEFIRNHISDYLDSLIQAQKDKGLIDDNTPVKTLPSYPFAHWANTDFNYVNGWSNPKYSSATKPDYRADVKTEMKYGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBZ1</accession>
    <name>A0A1A0HBZ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_41801</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the histone H2B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712010.1">
        <property type="nucleotide sequence ID" value="XM_018857452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBZ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBZ1"/>
    <dbReference type="GeneID" id="30030428"/>
    <dbReference type="OrthoDB" id="10254238at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22910">
        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000014">
        <property type="entry name" value="Histone H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000558">
        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23428">
        <property type="entry name" value="HISTONE H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00621">
        <property type="entry name" value="HISTONEH2B"/>
    </dbReference>
    <dbReference type="SMART" id="SM00427">
        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00357">
        <property type="entry name" value="HISTONE_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11 12" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA21500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="130" mass="14129" checksum="91B6F3B3DA530218" modified="2016-10-05" version="1">MAPKAEKKPASKAPAEKKPAAKKTASSGEPKKKSKTRKETYSSYIYKVLKQTHPDTGISQKAMSIMNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSTAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC55</accession>
    <name>A0A1A0HC55_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711972.1">
        <property type="nucleotide sequence ID" value="XM_018855957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC55"/>
    <dbReference type="GeneID" id="30028933"/>
    <dbReference type="OrthoDB" id="2122015at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007008">
        <property type="term" value="P:outer mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035195">
        <property type="entry name" value="Emr1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17237">
        <property type="entry name" value="Emr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="55" mass="6418" checksum="EF171D5D8BC19FA9" modified="2016-10-05" version="1">MIIASIRRIFTSFRTEEEEKAYSRHAFFNFLGFLGSCVIFSFVAQKLSKQPQQLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC93</accession>
    <name>A0A1A0HC93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_229836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712028.1">
        <property type="nucleotide sequence ID" value="XM_018855233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC93"/>
    <dbReference type="GeneID" id="30028209"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="511" mass="56508" checksum="908D919DF96CE00C" modified="2016-10-05" version="1">MSFWASKKPRPQSARFSFGSSLHHGIRNIGARRASAFGGVERDKNKWQFMPNKTREPQTKAGLKKSAKSQLTAFSDWLARLMSYFSSSCPHSDISTRDILYPCNLDLLYSIGMFSNNFAGCESKASMLKENGAASPASINCRSTHESPFLKLMAQKAREFTAVLSQKLNGSTSEHMQSNHLAKERLPYRLSSIFIPWGYGQHSASMTSANGRHNGDITSFSLYSRDCPAIEDISNSNNIVCDTGKNPLPRDPECPSAYDPSIKSLVNELFNDFMGNGCKEKCTLATENGCLTSTNPGPLQTRLTYFLGILPITFLSSKEATLEAVVPRSKYLGLDHQRKDSQNQHSHVLKNEEKTTTQSATSEIGRRSKICSVLDLSSLGNWEDEEMVLAGLQLGDTDLQQSTAKPTIEHENLFLTHPENFPRTQDTKVLDTSNSNMDDLSSIMSLLIARHLDQYEFADSATLASMGLVSMGLSFEEGSFSWVSSPVLQLKDEKAKSNSSRPIIQIFEGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCE9</accession>
    <name>A0A1A0HCE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DHH phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78489</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712072.1">
        <property type="nucleotide sequence ID" value="XM_018858887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCE9"/>
    <dbReference type="STRING" id="869754.A0A1A0HCE9"/>
    <dbReference type="GeneID" id="30031863"/>
    <dbReference type="OrthoDB" id="374045at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006798">
        <property type="term" value="P:polyphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF39">
        <property type="entry name" value="EG:152A3.5 PROTEIN (FBGN0003116_PN PROTEIN)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="5">
        <location>
            <begin position="231"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="395" mass="44591" checksum="734754376A931737" modified="2016-10-05" version="1">MTIKTFLEGLRISPSSKHVVRFVAGNQSADMDSVVSAITYAYFHHRFNPKEQALLPLVNISREEFRLRKDIVFLLSKHEISSTRIFFLDDVEELTQLSPSIKLDVVLVDHCNLQGKVLTGLYENKRLFVSGIIDHHADEGVFLDAMPRVIQSNGSCSALVYHFWNNKNGPITDKDAVLLLVGPLLIDTSNMEQKVEAGDREAYVEYGQILQSPEALMQTDGVAPAAFSQLYSELKAAKKDLRGFSLYDILRKDYKQFKFRGKLGDTVNVGFSSIGKSLSWVLGKFSVSEVLESLKMMTESFHLDVVVITSSFSQKDTKIYTREFCFSTLKASLEPLASLAQELELTSRFYKDDTVARQMAQINGSVFFRVYDQQNTAASRKQVVPIIKQVVEQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI6</accession>
    <name>A0A1A0HCI6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_229914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712215.1">
        <property type="nucleotide sequence ID" value="XM_018855235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCI6"/>
    <dbReference type="GeneID" id="30028211"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.130:FF:000004">
        <property type="entry name" value="cytosolic iron-sulfur assembly component 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="31114" checksum="BAF6428166AF6050" modified="2016-10-05" version="1">MRMPLFYTPFSIRRALRSNLFAVDSAKKQLGREHRFHDSTLLDSSSVQSSFFLHNIRVHITRVMSDPINANPTIIDVSSLPTRLDTLSKEKDAKRELVDRILSLSLYPQINSVAPPDEVPELISEGELSATSDEELDFDEDDEDEEIIDAQEIFDLIATISDPEHPLTLAQLAVVNLSDITVTHGTDKKRDISEVLIKITPTITHCSLATLIGLGIRVRLERLLPPRFRIRILIKEGTHQSENQVNKQLNDKERVAAACENDQLLSVILQMLSTCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD22</accession>
    <name>A0A1A0HD22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_39886</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712273.1">
        <property type="nucleotide sequence ID" value="XM_018857195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD22"/>
    <dbReference type="STRING" id="869754.A0A1A0HD22"/>
    <dbReference type="GeneID" id="30030171"/>
    <dbReference type="OrthoDB" id="5215911at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF30">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G04702)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="451"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="491"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="521"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="63241" checksum="305EB17906889594" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD44</accession>
    <name>A0A1A0HD44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">FAD-binding FR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_40351</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712313.1">
        <property type="nucleotide sequence ID" value="XM_018857262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD44"/>
    <dbReference type="STRING" id="869754.A0A1A0HD44"/>
    <dbReference type="GeneID" id="30030238"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508826" description="FAD-binding FR-type domain-containing protein" evidence="12">
        <location>
            <begin position="19"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="312"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="13">
        <location>
            <begin position="274"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding 8" evidence="14">
        <location>
            <begin position="425"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric reductase NAD binding" evidence="15">
        <location>
            <begin position="529"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA21817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="703" mass="80685" checksum="5EAAEC3AF115D2D5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDM0</accession>
    <name>A0A1A0HDM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AFG1-like ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_77536</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AFG1 ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712519.1">
        <property type="nucleotide sequence ID" value="XM_018858718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDM0"/>
    <dbReference type="STRING" id="869754.A0A1A0HDM0"/>
    <dbReference type="GeneID" id="30031694"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040713">
        <property type="entry name" value="ZapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169:SF6">
        <property type="entry name" value="AFG1-LIKE ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="ATPASE N2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="472" mass="54302" checksum="748BE10D9A084BBA" modified="2016-10-05" version="1">MLLSRLAPAKQPRTWRPRYTPLEAYDRRVALHKLTDDPYQRKILLSLSSLHAQLKAYRPPPVATPDVEDLKPKTGLSKFWSGMLRRRERTRDGQPSLVKGIYLWGDVGCGKTMLMDLFYLTVPASLPKRRFHFHQFMQNLHKRSYQLKREHGHADLDVIPLLASEIAKTATVLCFDEFQVTDVADAMLLRRLLTLLLSPQYGVVLFATSNRAPDDLYLNGIQRVLFIPCIRMIKNETRVIYLNSPTDYRKMPRPFSSVYYFPNPGVKYHSKASASARQKHCDKWYEYFNQANHDKAVLHDDSITVWGRTLHVPASSRPYVAQFTFEELCGKNLSAGDYLSLAHTYRAFVVTDIPYLSIDVRDTVRRFITFLDAVYDAHGCIATTAAAAFNDLFVEPENLLKDNFLLYKRQSDSGTEETFEYDDLVVKHGFDRLVAKKASMFANDEEKFAFARALSRLTQMGTTDWLEESRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDM8</accession>
    <name>A0A1A0HDM8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_146572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712617.1">
        <property type="nucleotide sequence ID" value="XM_018854528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDM8"/>
    <dbReference type="GeneID" id="30027504"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="95" mass="10118" checksum="C2B6771B8908E355" modified="2016-10-05" version="1">MLVRCLHPRHVTRAVCGVLPANIGAGEGPGIGGSLLILAAFVFPCISPSLGVLYLNAFKIVALIGVLSKLCFSTRVVFIWVFFNLGVFLFASLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDT4</accession>
    <name>A0A1A0HDT4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_147235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712636.1">
        <property type="nucleotide sequence ID" value="XM_018854532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDT4"/>
    <dbReference type="GeneID" id="30027508"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="26030" checksum="9DD012D701B93ED4" modified="2016-10-05" version="1">MSFNANDNSNINDHSHQYHHHGHQDHHDHRNHHDHHEHHDHDHKSTLKKVGDKIKGVLGKDSSDDDGSVGKTQNTTVPGESRRPSAGNPVSIPSESNEIHSPSGRAEGISPSSKDNHSLSENTQDSYGSHMPPQESFGVAGGDFWSSGNQYKLTKTEYEPAAGMDDPFQNSGDAYGSSERTSTGVPGEGGDIYGARRAQESLGSFDDAFGSPGGGKHDYEGPSKGFGTTGGGGDSYGSGYVEYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDV0</accession>
    <name>A0A1A0HDV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31195</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712765.1">
        <property type="nucleotide sequence ID" value="XM_018855803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDV0"/>
    <dbReference type="STRING" id="869754.A0A1A0HDV0"/>
    <dbReference type="GeneID" id="30028779"/>
    <dbReference type="OrthoDB" id="2740448at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668:SF18">
        <property type="entry name" value="MALTOSE FERMENTATION REGULATORY PROTEIN MAL13-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="10">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="742" mass="84594" checksum="A6F34527E5FB6551" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE74</accession>
    <name>A0A1A0HE74_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_31210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712786.1">
        <property type="nucleotide sequence ID" value="XM_018855809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE74"/>
    <dbReference type="GeneID" id="30028785"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="11608" checksum="1671F59BD0BCCB44" modified="2016-10-05" version="1">MNHDSGAESWGLFVAMHPREQMNKTYESNKIQKNKTNTKPIQDKKPMQKKKHIINRTKEKIPAFGRTRGWLSGPSEVTVAGIGLGPAWTDPRRLAPGPHLPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE75</accession>
    <name>A0A1A0HE75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Signal recognition particle 54 kDa protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_53823</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPase-activating proteins (GAPs), thereby resolving their association.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Fungal signal recognition particle consists of a 7S RNA molecule (scR1) and at least six protein subunits: srp72, srp68, srp54, sec65, srp21 and srp14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">The M domain binds the 7SL RNA and the signal sequence of presecretory proteins.</text>
    </comment>
    <comment type="domain">
        <text evidence="13">The NG domain, also named G domain, is a special guanosine triphosphatase (GTPase) domain, which binds GTP and forms a guanosine 5'-triphosphate (GTP)-dependent complex with a homologous NG domain in the SRP receptor subunit srp101. The two NG domains undergo cooperative rearrangements upon their assembly, which culminate in the reciprocal activation of the GTPase activity of one another. SRP receptor compaction upon binding with cargo-loaded SRP and GTPase rearrangement drive SRP-mediated cotranslational protein translocation into the ER.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712698.1">
        <property type="nucleotide sequence ID" value="XM_018858290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE75"/>
    <dbReference type="STRING" id="869754.A0A1A0HE75"/>
    <dbReference type="GeneID" id="30031266"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006325">
        <property type="entry name" value="SRP54_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01425">
        <property type="entry name" value="SRP54_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="15">
        <location>
            <begin position="288"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA22202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="555" mass="60526" checksum="E5ACB1060B528422" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE84</accession>
    <name>A0A1A0HE84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATP-dependent RNA helicase SUB2</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent RNA helicase sub2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">METBIDRAFT_39820</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ATP-binding RNA helicase involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 also plays a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712796.1">
        <property type="nucleotide sequence ID" value="XM_018857183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE84"/>
    <dbReference type="STRING" id="869754.A0A1A0HE84"/>
    <dbReference type="GeneID" id="30030159"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000346">
        <property type="term" value="C:transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000809">
        <property type="entry name" value="ATP-dependent RNA helicase SUB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 24" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="23">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="79"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="22">
        <location>
            <begin position="266"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="19">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OBA22300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="432" mass="48790" checksum="64BC954DCAF35C41" modified="2016-10-05" version="1">MSHEGQEELLDYSDSEEIAVQPAKTQADEAAGNKDADKKGSYVGIHATGFRDFLLKPELLRAIGDCGFEHPSEVQQVCIPQSILGTDVLCQAKSGLGKTAVFVLSTLQQLDPIPGEISTLVICHTRELAYQIRNEYARFSKYMPDVKTDVFYGGVPITRDLEKLQNKDTCPQIVVATPGRLHALVAEKGIRLNNIKSFVVDECDKVLESLDMRRDVQDIFRNTPHQKQVMMFSATLSQEIRPVCKKFMQSPLEIFVDDKAKLTLHGLQQYYLKLAEKEKNRQLSALLDTLEFNQVIIFVKSTHRANELNKLLCACNFPSIAIHSGLKQEERIARYKSFKEFNKRICVSTDVFARGIDIERINLAINYDLPSEADQYLHRVGRAGRFGTKGLAVSFVSSQEDEEVLEKIQSRFDVKITEFPEEGVDPSTYMNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEL9</accession>
    <name>A0A1A0HEL9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_77769</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712833.1">
        <property type="nucleotide sequence ID" value="XM_018858760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEL9"/>
    <dbReference type="STRING" id="869754.A0A1A0HEL9"/>
    <dbReference type="GeneID" id="30031736"/>
    <dbReference type="OrthoDB" id="44467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071913">
        <property type="term" value="F:citrate secondary active transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006843">
        <property type="term" value="P:mitochondrial citrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000007">
        <property type="entry name" value="Mitochondrial tricarboxylate transport protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049563">
        <property type="entry name" value="TXTP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788">
        <property type="entry name" value="SUCCINATE/FUMARATE MITOCHONDRIAL TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45788:SF4">
        <property type="entry name" value="TRICARBOXYLATE TRANSPORT PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="10"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="108"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="10">
        <location>
            <begin position="208"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="296" mass="31447" checksum="8C7EB801E5C4A140" modified="2016-10-05" version="1">MSKNPEKSSIHPFKSFVAGGTAGAIEGVITYPFEFAKTRLQLVDKSSKASRNPLVLITNVAKTQGVSSLYVGCPAFVVGNTVKALVRFLGFDSMKALLADRDGKLSGPRGVLAGLGAGLLESVIAVTPFEAIKTALIDDKQAAKPKYQSGLVSGTVKLVRDMGFRGMYAGVVPVSLRQASNQAVRLGSYNAIKTMIQLATNTKASEPLSSAATFAVGSFAGIVTVYTTMPIDTVKTRMQALGADKLYSSTLNCFVKIFKEEGLLTFWKGATPRLGRLVLSGGIVFTIYEKMLVLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEX9</accession>
    <name>A0A1A0HEX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_40375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713052.1">
        <property type="nucleotide sequence ID" value="XM_018857265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEX9"/>
    <dbReference type="STRING" id="869754.A0A1A0HEX9"/>
    <dbReference type="GeneID" id="30030241"/>
    <dbReference type="OrthoDB" id="1284551at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045159">
        <property type="entry name" value="DCAF7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19919">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="393"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="96"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="475" mass="50465" checksum="2C94D43B0636DF23" modified="2016-10-05" version="1">MDWNTLRSKRMSISGIPALAKPPLQPLLRFARPSEYSLGSEPYLQHAPAEARPSCYTSQHPLFCADWTHGAAGELVALGLFREGFQNRIQIVSGRADRDRAGDSNLGPGDSNLGPGDSNLGPGSRNASPAGRELGAPGGAGSSARGSTFDFACVAETLVDYPATALQWEPHGHERLAVSSEVLRLYKLGLDYGEPALVQTHMLANNSTANAASGGGNGAAAHDINTFPPVTSFDWNAADPSIVITLSVDTTCTVWDLSRSHAAGSHDTAHVKTQLIAHDLEVFDVKFLHMSTNVFASVSNDGSMRAFDLRSLEHSTIIYEPTGPAPPAADSPAASAQYNPQALLRLLASCADQHHLATVGVNLNQVLIIDLRMPGVPVVTIDGLFDGQSSAPINLLQWHPTANLLATGGDDCQALVWDCSGRTGHRPAADSKVVIDTPVLAYSADYEVNTVCWRRGCDNWMGVVSGKGFQALETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEY0</accession>
    <name>A0A1A0HEY0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Arad-like aldolase/epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712943.1">
        <property type="nucleotide sequence ID" value="XM_018857173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEY0"/>
    <dbReference type="STRING" id="869754.A0A1A0HEY0"/>
    <dbReference type="GeneID" id="30030149"/>
    <dbReference type="OrthoDB" id="3238794at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.225.10:FF:000009">
        <property type="entry name" value="Class II aldolase/adducin N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051017">
        <property type="entry name" value="Aldolase-II_Adducin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004855">
        <property type="entry name" value="PRK06208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672">
        <property type="entry name" value="ADDUCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10672:SF25">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 14 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="1">
        <location>
            <begin position="49"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="290" mass="31358" checksum="75D6DCF13C4A2E61" modified="2016-10-05" version="1">MSPSADQAITAKGYKIESAGANNMSKGGANPHVPPTFGDKYEQRRWMLEHASAAFRVFARFGYSEGEAGHISLRDPVDPTTFFINPLGVHFAMISPADLVHVDRKGNILPDGNQAPINAAGFAIHLSLQEARPDINAACHAHSVYGKAYSAFGQPIEMLNQDACVFYNTQAVYKNFGGVAIEGDEGQEIADAAGGARAVILQNHGLLTMGKTVDEAAYLFMLMEKSCQVQLLADAAAAGAKRAKVLIPEDVCAYSADVTNHPDVMYSSFQSEYSYQRRLDKDEFLCAKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFJ4</accession>
    <name>A0A1A0HFJ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glucose starvation modulator protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77981</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor which regulates nonfermentable carbon utilization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERT1/acuK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713129.1">
        <property type="nucleotide sequence ID" value="XM_018858798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFJ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFJ4"/>
    <dbReference type="GeneID" id="30031774"/>
    <dbReference type="OrthoDB" id="2538135at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050335">
        <property type="entry name" value="ERT1_acuK_gluconeogen_tf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659:SF8">
        <property type="entry name" value="GLUCOSE STARVATION MODULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47659">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="12">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS00463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="424" mass="48204" checksum="BD13C8A1B56D19E2" modified="2016-10-05" version="1">MTRKLTPGEKVGRKPTLRACTFCHLKHLQCLNTRPCQNCVKRKIEHGCKDIVRKRALYLLPGRELKISRKNSAAASEHRSSSSASSSLETPGSTPSTTANFHSMFLNQEYMMLGDLILKPLLPSPAFAPVPGQLVTPEFFDGLDGMGDGAESRPYISLGDGAGKLARENGFNFDLRASDDQEYVLPLLSHHIYQTVQDIYSNRIINFDYPLSYHSLTFFLKNRFSVASKHATPEVRARKRHNLLVILKLIASYRPTFISAHKSLFQPFDFQFLEMLFQRCLLDFENLSRLNASPMIIWRRTGEIVLMLAHLADILGLNISDVLSKRTFIIELMYDDESIVEYFRLFKSVAIGSLHLTIFTKCKLIKRPSDHRMSTSKTNAQLTDVAFIEFCSVWTVKRDLFDLPMMIVGHFLPSLPNSDAVRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG15</accession>
    <name>A0A1A0HG15_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19 20">ubiquitin carrier protein UBC2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17 21">ubiquitin-protein ligase UBC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ORF">METBIDRAFT_36585</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="26">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713318.1">
        <property type="nucleotide sequence ID" value="XM_018856761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG15"/>
    <dbReference type="STRING" id="869754.A0A1A0HG15"/>
    <dbReference type="GeneID" id="30029737"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990304">
        <property type="term" value="C:MUB1-RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097505">
        <property type="term" value="C:Rad6-Rad18 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990305">
        <property type="term" value="C:RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990303">
        <property type="term" value="C:UBR1-RAD6 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034620">
        <property type="term" value="P:cellular response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042275">
        <property type="term" value="P:error-free postreplication DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031571">
        <property type="term" value="P:mitotic G1 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000639">
        <property type="term" value="P:negative regulation of SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090089">
        <property type="term" value="P:regulation of dipeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009302">
        <property type="term" value="P:sno(s)RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120174">
        <property type="term" value="P:stress-induced homeostatically regulated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000007">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 23" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0749">Sporulation</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="25">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="22">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00041569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00042190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00080892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="OBA22837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="168" mass="19116" checksum="4AD624FF051E01C1" modified="2016-10-05" version="1">MSTPAKRRLMRDFKRMQLDAPSGVSALPVPDNVMSWNAVIIGPAETPFEDGTFRLGLQFDEQYPNKPPLVKFLSEMFHPNVYASGELCLDILQNRWSPTYDVSSILTSIQSLLNDPNISSPANVEAANLYKDHRSQYIKRVRETVENSWNEEDDDSDDSDEDEMEEDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG51</accession>
    <name>A0A1A0HG51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">METBIDRAFT_38149</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713620.1">
        <property type="nucleotide sequence ID" value="XM_018856961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG51"/>
    <dbReference type="STRING" id="869754.A0A1A0HG51"/>
    <dbReference type="GeneID" id="30029937"/>
    <dbReference type="OrthoDB" id="10261066at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000001">
        <property type="entry name" value="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 24" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="177"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="206"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="305"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="472"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="22">
        <location>
            <begin position="24"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="23">
        <location>
            <begin position="539"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="719"/>
            <end position="749"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00059243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00067960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="OBA23139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="749" mass="83843" checksum="37F83824F3E82DBF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG75</accession>
    <name>A0A1A0HG75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_29548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713487.1">
        <property type="nucleotide sequence ID" value="XM_018855432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG75"/>
    <dbReference type="STRING" id="869754.A0A1A0HG75"/>
    <dbReference type="GeneID" id="30028408"/>
    <dbReference type="OrthoDB" id="4096268at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="2">
        <location>
            <begin position="25"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="311"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="618" mass="67448" checksum="45D4B17B0BD9F44E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGH5</accession>
    <name>A0A1A0HGH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Autophagy-related protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37604</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for the Cvt vesicles completion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Preautophagosomal structure membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Vacuole membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="7">During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the ATG11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713587.1">
        <property type="nucleotide sequence ID" value="XM_018856896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGH5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGH5"/>
    <dbReference type="GeneID" id="30029872"/>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990316">
        <property type="term" value="C:Atg1/ULK1 kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903599">
        <property type="term" value="P:positive regulation of autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034517">
        <property type="term" value="P:ribophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040040">
        <property type="entry name" value="ATG11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019460">
        <property type="entry name" value="Atg11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045326">
        <property type="entry name" value="ATG17-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13222:SF1">
        <property type="entry name" value="RB1-INDUCIBLE COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10377">
        <property type="entry name" value="ATG11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04108">
        <property type="entry name" value="ATG17_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0926">Vacuole</keyword>
    <feature type="domain" description="Autophagy protein ATG17-like" evidence="9">
        <location>
            <begin position="40"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy-related protein 11 C-terminal" evidence="10">
        <location>
            <begin position="787"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="473"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="924" mass="104449" checksum="96620E3E8679B778" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGK5</accession>
    <name>A0A1A0HGK5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U1 small nuclear ribonucleoprotein component SNU71</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_63364</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the U1 snRNP particle, which recognizes and binds the 5'-splice site of pre-mRNA. Together with other non-snRNP factors, U1 snRNP forms the spliceosomal commitment complex, that targets pre-mRNA to the splicing pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNU71 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713606.1">
        <property type="nucleotide sequence ID" value="XM_018858330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGK5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGK5"/>
    <dbReference type="GeneID" id="30031306"/>
    <dbReference type="OrthoDB" id="6275295at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002483">
        <property type="entry name" value="PWI_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01480">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00311">
        <property type="entry name" value="PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="PWI" evidence="7">
        <location>
            <begin position="529"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="57"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="334"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="277"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="460"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="600" mass="65867" checksum="39C5F14947AB1696" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGM5</accession>
    <name>A0A1A0HGM5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_63409</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713626.1">
        <property type="nucleotide sequence ID" value="XM_018858331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGM5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGM5"/>
    <dbReference type="GeneID" id="30031307"/>
    <dbReference type="OrthoDB" id="10262653at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1740.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047864">
        <property type="entry name" value="Ribosomal_eS26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00733">
        <property type="entry name" value="RIBOSOMAL_S26E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="103"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="119" mass="13638" checksum="B3CCA1D302F081E7" modified="2016-10-05" version="1">MPKKRASNGRNKKGRGHVKPVRCLNCARCVPKDKAIKRVTIRNMVEAAAVRDLSEASVYQEYALPKLYNKLHYCVSCAIHARIVRVRSRVDRRVRTPPQRKRFTQDKKVSPQDAAKKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGV7</accession>
    <name>A0A1A0HGV7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_98523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713564.1">
        <property type="nucleotide sequence ID" value="XM_018859473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGV7"/>
    <dbReference type="GeneID" id="30032448"/>
    <dbReference type="OrthoDB" id="4022548at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="163" mass="17880" checksum="A7269787DCCE7C33" modified="2016-10-05" version="1">MPSGGVLLLQFLVKPRGQSHVRGSRFRRSNRTPAEAAAVVSNSKVEPAAAAFSYDSPVVIAPQLFRSVTYWNQRSHVSFIPTPVVSLGVTPQTTGRPLLLHIFGPGPERSIEHTVFLEFFIPGLSGLSAFDFVVYESRVAPLVVGEDFWVAFKVLCQPRNVSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGZ4</accession>
    <name>A0A1A0HGZ4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_100961</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eL38 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713633.1">
        <property type="nucleotide sequence ID" value="XM_018853684.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGZ4"/>
    <dbReference type="GeneID" id="30026660"/>
    <dbReference type="OrthoDB" id="10250488at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022618">
        <property type="term" value="P:protein-RNA complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.720.90:FF:000002">
        <property type="entry name" value="60S ribosomal proteins L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.720.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002675">
        <property type="entry name" value="Ribosomal_eL38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038464">
        <property type="entry name" value="Ribosomal_eL38_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10965:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01781">
        <property type="entry name" value="Ribosomal_L38e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="8980" checksum="56DB4BFDE99FFCE1" modified="2016-10-05" version="1">MAREIKDIKEFVELARRADITSATVKVNKKVNPKGKTFKQTKFKVRGSRYQYTLVVNDTAKAKKLQQSLPPSLEIKTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGZ6</accession>
    <name>A0A1A0HGZ6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA polyadenylation factor FIP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_228395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713746.1">
        <property type="nucleotide sequence ID" value="XM_018855205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGZ6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGZ6"/>
    <dbReference type="GeneID" id="30028181"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="7">
        <location>
            <begin position="144"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="45"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="297" mass="31795" checksum="8B3FA22C6562AC15" modified="2016-10-05" version="1">MPNSDDEDAYLYLSEDDRPAAKKPRLELPTGPAASRMKTARSAADEDDDDDDEESSDDDVEFVIGDSAPRAPANATTTVSTTSGALNDAIGEVNDEDDDTDEKPGARGASSARKKTIRVSKDDGGATVDINSVAEYEGKPLTQLDLDEIKEKPWRAPGADISDYFNYGFDEFTWTAYCHKQDKLRGEFNPQKVMAKMMGGVSGGKGQMPFPFPFMPNMPNMPNMPKMPNMPMPPNMAMPPNMAMPPNMAGMPMPPGMGNGPPGSGFGPGNPPGSGNLPGGPSGKQGSRQQGHSRYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH97</accession>
    <name>A0A1A0HH97_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3beta-hydroxysteroid 3-dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.270</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_102085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="6">Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 5/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the short-chain dehydrogenases/reductases (SDR) family. ERG27 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.270" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713694.1">
        <property type="nucleotide sequence ID" value="XM_018853711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH97"/>
    <dbReference type="STRING" id="869754.A0A1A0HH97"/>
    <dbReference type="GeneID" id="30026687"/>
    <dbReference type="OrthoDB" id="9989144at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000253">
        <property type="term" value="F:3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051593">
        <property type="entry name" value="Ergosterol_Biosynth_ERG27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647:SF1">
        <property type="entry name" value="3-KETO-STEROID REDUCTASE ERG27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43647">
        <property type="entry name" value="DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0752">Steroid biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="343" mass="38857" checksum="D63511337B4FD55C" modified="2016-10-05" version="1">MREGKVAVITGTNSNLGINIAYRLLDEISPSTNLTIVVTSRTLPRVQDVISKIKKHAREKLPHRTGFLEFDYLLVDFTNMVSILGAYYDLEKKFQHIDFVFVNAAQGCYSGIDWIAAFQCIFTNLLDAVTFPTYKIQRIGVKSDDDMGLVFQGNAFGPYYFLHKIKALLAGGGRIIWISSVMSGPEYLSFNDLQLLKSHEPYEGSKRLVDLIHTGSYKKLKSEHGILSYVVHPGIFTSFSFFQYLNFLTYYGMMFMFYMARFMGSQIHNISGYTAANSPVCVALNNKEQDAKLASSCDRWGKEFIDKAEIDPTGAEDVIAYMDKLVSEWDETLKHQITPTRIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHA0</accession>
    <name>A0A1A0HHA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thump-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_29862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713857.1">
        <property type="nucleotide sequence ID" value="XM_018855498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHA0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHA0"/>
    <dbReference type="GeneID" id="30028474"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180014">
        <property type="term" value="F:protein-tRNA adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051391">
        <property type="term" value="P:tRNA acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11717">
        <property type="entry name" value="THUMP_THUMPD1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2300.10:FF:000001">
        <property type="entry name" value="THUMP domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="3">
        <location>
            <begin position="148"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="286" mass="32764" checksum="89784A2BD4C4ADF4" modified="2016-10-05" version="1">MGKRRSDSGNGNKNKKYKVSCFIDPNTMGIYATCNRGRELGCRKELVNLLSEKIDKLYPDWEKHVEEEEVEANDEDRKNSEEKTADTKELSVEDQIKQELDEMKGPQGRKKSVHPLLHPMELGCECLVFVKVRKPAVPVELVQSLCEEATLTKQKNTRFTQKLTPITFSVSASMEELTKLAEKVLKPHFHAEDQVPVKFAIQVTRRNFNSLEKDDIIKQVATSVGRENGHTVDLKNYDKLIMVECFKNNIGMSVVSDFKKYEKFNLQQIFEKDMGESTLSRVSNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHB7</accession>
    <name>A0A1A0HHB7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone-lysine N-methyltransferase, H3 lysine-4 specific</fullName>
            <ecNumber evidence="3">2.1.1.354</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">COMPASS component SET1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">SET domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_30013</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60260"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.354"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60272"/>
            <dbReference type="Rhea" id="RHEA-COMP:15537"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(6)-methyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60268"/>
            <dbReference type="Rhea" id="RHEA-COMP:15540"/>
            <dbReference type="Rhea" id="RHEA-COMP:15543"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.354" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714055.1">
        <property type="nucleotide sequence ID" value="XM_018855532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHB7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHB7"/>
    <dbReference type="GeneID" id="30028508"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140999">
        <property type="term" value="F:histone H3K4 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024657">
        <property type="entry name" value="COMPASS_Set1_N-SET"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044570">
        <property type="entry name" value="Set1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017111">
        <property type="entry name" value="Set1_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024636">
        <property type="entry name" value="SET_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45814:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SETD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11764">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11767">
        <property type="entry name" value="SET_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037104">
        <property type="entry name" value="Histone_H3-K4_mtfrase_Set1_fun"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01291">
        <property type="entry name" value="N-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51572">
        <property type="entry name" value="SAM_MT43_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 19" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7 19" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="17">
        <location>
            <begin position="906"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="18">
        <location>
            <begin position="1032"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="322"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="543"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="839"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="358"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="543"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="563"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="594"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="624"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="684"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="851"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030093"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA23574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1047" mass="118926" checksum="529384595B57B6CF" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHT6</accession>
    <name>A0A1A0HHT6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30006</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018855531.1"/>
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    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070336">
        <property type="term" value="F:flap-structured DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000736">
        <property type="term" value="P:double-strand break repair via single-strand annealing, removal of nonhomologous ends"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021624">
        <property type="entry name" value="Saw1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11561">
        <property type="entry name" value="Saw1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="203" mass="23165" checksum="CD55617E41C73848" modified="2016-10-05" version="1">MPATVTYISVAPGTAIPVRVFVNRKKILLLSEVAVNEKSIIKLSLINLIRLSNFDLTNLLLDVGSQLRRVLLETPLAELFPQRSVLRLVKSVSELSNTWTCKVVVSLGYLADLRFKLERLQVPADIRFLEDKKIEIDHRDTRGSALLEKSVKFDFTRREPASDDETSPLDDKKSISYKLQKQRLINNNVVDPLDLYVLHRPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHX1</accession>
    <name>A0A1A0HHX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GrpE protein homolog, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_51113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GrpE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714240.1">
        <property type="nucleotide sequence ID" value="XM_018858286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHX1"/>
    <dbReference type="STRING" id="869754.A0A1A0HHX1"/>
    <dbReference type="GeneID" id="30031262"/>
    <dbReference type="OrthoDB" id="201635at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000774">
        <property type="term" value="F:adenyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00446">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.22.10:FF:000002">
        <property type="entry name" value="GrpE protein homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.22.10">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01151">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000740">
        <property type="entry name" value="GrpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013805">
        <property type="entry name" value="GrpE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009012">
        <property type="entry name" value="GrpE_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237">
        <property type="entry name" value="GRPE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21237:SF23">
        <property type="entry name" value="GRPE PROTEIN HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01025">
        <property type="entry name" value="GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00773">
        <property type="entry name" value="GRPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58014">
        <property type="entry name" value="Coiled-coil domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51064">
        <property type="entry name" value="Head domain of nucleotide exchange factor GrpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="144" mass="16432" checksum="A8CF43ED9C1EE58D" modified="2016-10-05" version="1">MKNHYARSIADFRNLQETTKKENQKAKDFALQKFAKDLLESVDNFALALNSVKQDTLEAHPEMKNLFDGVSMTRNVFEKTLEKHGIQKIDPMGEQFDPNHHEATFEIPQPDKEPGTVFHVQQPGFTLNSRVLRPAKVGLVKGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI54</accession>
    <name>A0A1A0HI54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_30113</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714166.1">
        <property type="nucleotide sequence ID" value="XM_018855560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI54"/>
    <dbReference type="STRING" id="869754.A0A1A0HI54"/>
    <dbReference type="GeneID" id="30028536"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006735">
        <property type="term" value="P:NADH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000009">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF16">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="152"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA23685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="342" mass="36050" checksum="E21A63622D079C1B" modified="2016-10-05" version="1">MVKVTVCGAAGGIGQPLSMLLKLNPQVSHLSLFDIVNARGVAADLSHVNTSARVTGHQPSSKDDKSAIVDALTDSDIVVIPAGVPRKPGMTRADLFNINALIIRDLIANVGKTCPNATILIISNPVNATVAIAASVLKKLGIFNPRKLLGVTTLDSVRAETFLGGLIGETPHTLKGKISVIGGHSGDTIVPLIHFDSSISAKVKDISETDYIEYVRRVQFGGDEVVKAKNGAGSATLSMAYAGYRFTEYVLDSFANKISKEGIPDSAYIYLPGVEGGINLSASLGVEFFSVPVVLRAGEVASFHAPEKLLRTKTENEMVRVAIDGLKGSIAQGINFVEGSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI57</accession>
    <name>A0A1A0HI57_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Calponin-homology (CH) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714330.1">
        <property type="nucleotide sequence ID" value="XM_018856695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI57"/>
    <dbReference type="STRING" id="869754.A0A1A0HI57"/>
    <dbReference type="GeneID" id="30029671"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="22"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="170" mass="19367" checksum="EEA593E5B3CC14F9" modified="2016-10-05" version="1">MNQNAHTNLDQDLRLSRTAKFERSEHEAAEWIFDTLQLLQTERNLYDVGEHGLAEILKDGKLLCRLGNLLGLPLAPTAKYRTSRMPFVQMENILFFLQTCDMIGVGHDEIFQTVDLFEAKDPYQVVVTLMSFSRKAHELNASAFPTLMGPKVAKVKPHVPFKPLSLRGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIL8</accession>
    <name>A0A1A0HIL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714480.1">
        <property type="nucleotide sequence ID" value="XM_018855622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIL8"/>
    <dbReference type="GeneID" id="30028598"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="110" mass="12103" checksum="B8DEE69484859AB9" modified="2016-10-05" version="1">MKSQDLIVLAASVVSISGMFTPGKGNPALGSLEIPEEKPFPKESLHPDLEEVPPPIKRPDSPPRNPFGQHDSKPNGELFQDWSQGPSKPETQEHEYQDIRYGGYGNPPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIN8</accession>
    <name>A0A1A0HIN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-activated protein kinase glycogen-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_114565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714356.1">
        <property type="nucleotide sequence ID" value="XM_018853993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIN8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIN8"/>
    <dbReference type="GeneID" id="30026969"/>
    <dbReference type="OrthoDB" id="5873279at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02859">
        <property type="entry name" value="E_set_AMPKbeta_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="34511" checksum="FBBB302B4E535149" modified="2016-10-05" version="1">MLKKSEDSELFEGFVRVNDKQKLVFKFVVDGSLWTTAEKYKVEFDEHGNKNNYIEAEELILDSTCNPGAKDSSSVEINAYEKRRSMEGLVAKAALDFNKTPVENDESKVDEDYGDQLGDDEAGDSSEDEKPYMSTTSAVPSTSEVYSTAPAAFVSLVSSTNCNVSRSPAINRDETSGCDSGIHEHHVIKQFSLDNGCDLENILLTESLFTEVSYVTSNSQSMGNHLSNLMNPEGQMKEGFMSVNTIFEPATDHPGGAGSPFSEQSEDDHNSCVSGNSDYRSDEEVDSIGISIEVEDPGSGVPLPGVFAKIRGLFHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIQ2</accession>
    <name>A0A1A0HIQ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_76688</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714199.1">
        <property type="nucleotide sequence ID" value="XM_018858571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIQ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIQ2"/>
    <dbReference type="GeneID" id="30031547"/>
    <dbReference type="OrthoDB" id="422663at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000464">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17949">
        <property type="entry name" value="DEADc_DDX31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="188"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="499"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="460"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="741"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="460"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="753"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="770"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="782" mass="86277" checksum="EE5089951E8E2CBE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIT3</accession>
    <name>A0A1A0HIT3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_30160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714229.1">
        <property type="nucleotide sequence ID" value="XM_018855568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIT3"/>
    <dbReference type="STRING" id="869754.A0A1A0HIT3"/>
    <dbReference type="GeneID" id="30028544"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="248" mass="27286" checksum="BB7F9BB1C03DC34A" modified="2016-10-05" version="1">MKLRMSGAEFRNIGKVDFTSFSQLGESSYNKGEIAFGICSNQTQSIPFVVASFGLWINRGKMIFQKGIGSMTELYVVGKSIVNESLAITYNGSICLYNTHWNTNMDIKGHDCVAVGSDSRLEISFARGVNAVQNTQTIYLESPASVLAISGLTSLLTPPFINIAGFGQHNWIDLDVEFNNLTTEYDYFENSGLLVITLSKRQVVQIQIGESYDLRYFKLTSGPAGSRLVYEPPSPNTPPNACSCKLIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIV7</accession>
    <name>A0A1A0HIV7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peptidase M18, aminopeptidase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30303</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the peptidase M18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714426.1">
        <property type="nucleotide sequence ID" value="XM_018855607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIV7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIV7"/>
    <dbReference type="GeneID" id="30028583"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF4">
        <property type="entry name" value="VACUOLAR AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 9" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="548" mass="59940" checksum="25BEEBA784B539CB" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIY1</accession>
    <name>A0A1A0HIY1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sphingolipid long chain base-responsive protein PIL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_30230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714323.1">
        <property type="nucleotide sequence ID" value="XM_018855580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIY1"/>
    <dbReference type="STRING" id="869754.A0A1A0HIY1"/>
    <dbReference type="GeneID" id="30028556"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097446">
        <property type="term" value="P:protein localization to eisosome filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000005">
        <property type="entry name" value="Sphingolipid long chain base-responsive pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF1">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="166"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="34845" checksum="F275FC45F249E471" modified="2016-10-05" version="1">MHRTYSMRSQRAPTAAQMQAPPPPTSSTKSKFFGKGSLSHSIRKSAGGAFGPELARKLAQLVKMEKNLMRSIEITASERKEVAKQLSIWGEGNDDDISDITDKLGVLIYEIGELEDQFIDRYDQYRITLKSIRDIEGSVQPSRERKQKITDQIAYLKYKDPQSPKINVLEQELVRAEAESLVAEAQLSNITREKLKIAFNYQFDSVREHAEKIALIAGYGKALLELLDESPVTPGETRAAYDGYEASKQIIIDAENALASWTFDSAVVKPTLSFAAHEEEEDYDEEDLADEAEHLKITEDQWAEENKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ59</accession>
    <name>A0A1A0HJ59_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_114929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714403.1">
        <property type="nucleotide sequence ID" value="XM_018854005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ59"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ59"/>
    <dbReference type="GeneID" id="30026981"/>
    <dbReference type="OrthoDB" id="338816at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="75" mass="8644" checksum="92A14C74D0A1A27B" modified="2016-10-05" version="1">MEFAYQNQPSKLAPKKIKYGTTPGGNLWKAQDAALRQLEEDAKKQRELESKKEKEGSRKRGFSTTRCQRTRTLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJE8</accession>
    <name>A0A1A0HJE8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase catalytic subunit A</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_30600</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714765.1">
        <property type="nucleotide sequence ID" value="XM_018855665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJE8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJE8"/>
    <dbReference type="GeneID" id="30028641"/>
    <dbReference type="OrthoDB" id="1676488at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030908">
        <property type="term" value="P:protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007868">
        <property type="entry name" value="Hom_end_hint"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007869">
        <property type="entry name" value="Homing_endonuc_PI-Sce"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05204">
        <property type="entry name" value="Hom_end"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05203">
        <property type="entry name" value="Hom_end_hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="15">
        <location>
            <begin position="568"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA24284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1078" mass="117236" checksum="E04D1D327F8CF262" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJG2</accession>
    <name>A0A1A0HJG2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_34866</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714459.1">
        <property type="nucleotide sequence ID" value="XM_018856531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJG2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJG2"/>
    <dbReference type="GeneID" id="30029507"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000048">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="92"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="8">
        <location>
            <begin position="363"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="436" mass="48968" checksum="F19F4A04EA71640B" modified="2016-10-05" version="1">MPLFEFDDEDLASDLGSIGFEADSPAPVQNETPDADVVCTTIPPSRRRSTIYRRVLVRSSAPNSGGIAIPADFDSAPEEESAAAPKKCLADFKPLKVLGQGAYGKVHLVQDRLDGKLYAQKQIRKPQVDVFEYKPSVAANHVRRTIAERQILSEITHHKSIVKLFYALQDEGKFYLLLEYIPGGELFHHLTVNNKLGNVFLEKDVAFYIAEMALGLKHLHLLGIVYRDLKPENCLLNSKGHLVLTDFGLSKNVGNDHDSACHSIIGTPEYMAPEILKGLAYGYAVDWWLLGCVMYDMLTSRPPFTGDNSKIICKRILKSDPLIPFYLSLDAKDLLSKLLRKDPKKRMAVDDRWAAFKKHRFFRKLNWGALEDQEAKPPLVPLITNPALAENFSDEFTGMRMSEYDGSNVQGPDVDHLFEGFSYTASESFLENYVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJG7</accession>
    <name>A0A1A0HJG7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_35489</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714785.1">
        <property type="nucleotide sequence ID" value="XM_018856614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJG7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJG7"/>
    <dbReference type="GeneID" id="30029590"/>
    <dbReference type="OrthoDB" id="10265243at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140018">
        <property type="term" value="P:regulation of cytoplasmic translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000003">
        <property type="entry name" value="Elongator complex protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="18">
        <location>
            <begin position="81"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="395"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA24304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="548" mass="62300" checksum="6314BFC16A74BC7E" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJL6</accession>
    <name>A0A1A0HJL6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">60S ribosome subunit biogenesis protein NIP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_30534</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NIP7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714686.1">
        <property type="nucleotide sequence ID" value="XM_018855655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJL6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJL6"/>
    <dbReference type="GeneID" id="30028631"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902626">
        <property type="term" value="P:assembly of large subunit precursor of preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.130.10:FF:000002">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.220:FF:000001">
        <property type="entry name" value="60S ribosome subunit biogenesis protein NIP7 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="95"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20679" checksum="222387ED0EF60284" modified="2016-10-05" version="1">MRSLTEEETKVVFEKLANYMGRNISFLIDNPTNPHVFRLQKDRVYYVSEHIAKFAVCVSRDRLMALGTCFGKFTKTGKFRLHITALPYLAQYAKYKVWIKQNGEMPYLYGNHVLKAHVGRIAEDIPEHAGVIVFLMHDVPLGFGVSAKSTAETKNMQPTAIVAFRQGDIGEYLREEDTLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJY3</accession>
    <name>A0A1A0HJY3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Jacalin-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714792.1">
        <property type="nucleotide sequence ID" value="XM_018856720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJY3"/>
    <dbReference type="GeneID" id="30029696"/>
    <dbReference type="OrthoDB" id="74460at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.100.10.30">
        <property type="entry name" value="Jacalin-like lectin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001229">
        <property type="entry name" value="Jacalin-like_lectin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036404">
        <property type="entry name" value="Jacalin-like_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053002">
        <property type="entry name" value="Metalloproteinase_M10B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021917">
        <property type="entry name" value="Unchr_Zn-peptidase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054:SF2">
        <property type="entry name" value="MIP04191P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21054">
        <property type="entry name" value="ZINC METALLOPROTEINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01419">
        <property type="entry name" value="Jacalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12044">
        <property type="entry name" value="Metallopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51101">
        <property type="entry name" value="Mannose-binding lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51752">
        <property type="entry name" value="JACALIN_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Jacalin-type lectin" evidence="1">
        <location>
            <begin position="547"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="720" mass="80115" checksum="4A2936C5C7F57C9B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJZ2</accession>
    <name>A0A1A0HJZ2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sld7 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_228880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714821.1">
        <property type="nucleotide sequence ID" value="XM_018855216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJZ2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJZ2"/>
    <dbReference type="GeneID" id="30028192"/>
    <dbReference type="OrthoDB" id="4063051at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041260">
        <property type="entry name" value="Sld7_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18596">
        <property type="entry name" value="Sld7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sld7 C-terminal" evidence="2">
        <location>
            <begin position="185"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="271" mass="29897" checksum="32CE9462C017CAD2" modified="2016-10-05" version="1" fragment="single">MPSTSFSLQTDRGLLADIQIWSDAATEQPNVDPHLDFVCVAYHERIPLFLVSNGPFDVYSEHVETENYFYESLVCLSEAVGILAKSLLEGTDTYFVFYLDQKALPRVRCYYIDFSVRQQVAEFREKASKIGDQDVFFISKTLGSAGRSDTSVFDRVLGTKASKIPGPVSAPQPPVAGTLALTTSQQISQAVSRVVLSGLRLRGLSTNPQSSANDKLAIREIYHMTNKAANFALRKYSYEFNERARQAPPADHVGGHPGHSGGVARDLCRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK67</accession>
    <name>A0A1A0HK67_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">PIG-P-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34344</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714877.1">
        <property type="nucleotide sequence ID" value="XM_018856466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK67"/>
    <dbReference type="STRING" id="869754.A0A1A0HK67"/>
    <dbReference type="GeneID" id="30029442"/>
    <dbReference type="OrthoDB" id="690928at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="6">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="160" mass="18330" checksum="E64334418F1F097C" modified="2016-10-05" version="1">MGFLTEDSLARESDVTVSTNVTLYAEYKGFFMYVLSAVALTMFVMWILAPHSVLLSLGISYYPDKYWAQAIPMYFLMLMFYLYVLVTLYNNEVQTLKLDDLRVFTDEHSVSPEDPVDWLWKAPSGVWDLPIGLVNEVLYGEDEDKIHYGDTPESGLKGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKB6</accession>
    <name>A0A1A0HKB6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">NTF2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_10585</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NXF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714927.1">
        <property type="nucleotide sequence ID" value="XM_018853805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKB6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKB6"/>
    <dbReference type="GeneID" id="30026781"/>
    <dbReference type="OrthoDB" id="25872at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042272">
        <property type="term" value="C:nuclear RNA export factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14342">
        <property type="entry name" value="UBA_TAP-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040736">
        <property type="entry name" value="Mex67_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057125">
        <property type="entry name" value="NXF1/2/3/5-like_LRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030217">
        <property type="entry name" value="NXF_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005637">
        <property type="entry name" value="TAP_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10662:SF22">
        <property type="entry name" value="NUCLEAR RNA EXPORT FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24048">
        <property type="entry name" value="LRR_NXF1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22602">
        <property type="entry name" value="NXF_NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18444">
        <property type="entry name" value="RRM_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03943">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00804">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51281">
        <property type="entry name" value="TAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="9">
        <location>
            <begin position="296"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="TAP-C" evidence="10">
        <location>
            <begin position="547"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="483"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="499"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA24446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="599" mass="65334" checksum="692006ACF9D1C286" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKG5</accession>
    <name>A0A1A0HKG5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3 9">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_34271</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic component of the histone acetylase B (HAT-B) complex. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the HAT-B complex composed of at least HAT1 and HAT2. The HAT-B complex binds to histone H4 tail.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HAT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 9"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714868.1">
        <property type="nucleotide sequence ID" value="XM_018856458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKG5"/>
    <dbReference type="STRING" id="869754.A0A1A0HKG5"/>
    <dbReference type="GeneID" id="30029434"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="13">
        <location>
            <begin position="16"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="12">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA24387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="405" mass="46686" checksum="1453E218679D3DEE" modified="2016-10-05" version="1">MESVGPAIASLQPELWTASLNEAITIYITDTAGSAQSFQPSFTYPIFGESETIFGYQDLQIFLCFDSVTFLPFLNVKWSQKLDEVDVDPKEKLLELLPESTVFKDELKWRDAIDAEQKEYKIPGEIVGDSWSKDGETYGIYKIDMSLKLGVELLRRLQILVLLFIEAGTYIEHDDAKWDIYVMYKTTDPKLPEIVGFATAYNYWSYPGAEKFEAGETRIRKKLSQFIVLPVFQGQLLGADMYAKLYDTWAAEPGVLEIVVEDPNESFDDLRDRVDFTRLVDTGKIDLNTFSIKVAQQDGFLNDFKTAEKLEKRQLQRLLEMALLYQLKHGLSRDSKKDVRLYIKKRLYEKNKEALSDMDEPTMLDKLQTAYLALEDDYYRILEPVKLSHKRKAATDGKPTKKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKN8</accession>
    <name>A0A1A0HKN8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DnaJ homologue subfamily C member 28 conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36175</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715037.1">
        <property type="nucleotide sequence ID" value="XM_018856702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKN8"/>
    <dbReference type="STRING" id="869754.A0A1A0HKN8"/>
    <dbReference type="GeneID" id="30029678"/>
    <dbReference type="OrthoDB" id="1922282at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018961">
        <property type="entry name" value="DnaJ_homolog_subfam-C_membr-28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394:SF1">
        <property type="entry name" value="DNAJ HOMOLOGUE SUBFAMILY C MEMBER 28 CONSERVED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39394">
        <property type="entry name" value="YALI0E31793P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09350">
        <property type="entry name" value="DJC28_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DnaJ homologue subfamily C member 28 conserved" evidence="1">
        <location>
            <begin position="199"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="436" mass="49416" checksum="73DDB49EEB692CB6" modified="2016-10-05" version="1">MRSFLNTCRNHKDNSALAERFEQILSQKIDESTKSFIDQNPTLQHLYTKYHKENDAKTFEAVQLQNNAQSKDYGAKFQKEIGLIKSANLIKVKDSEAILHPWTGTERVQDTTLRMLVDSAPKPKRLHRNKTLFTAPKTLGTRVTDAKEGSLDYKLSKVLTPEEKEKEEFREMYQERLLGPAMFAGHNSPTTASGMIGNLASARINSVINRKTGKFESPDMEKVRGKPLDRERLANSTNSNYFMNQILKNQECLPVWIESQQGIDKEISVFRLEIQKKIIKAIIDSASAHVGTVDADIIDYIITADSELLAGFARKELLKRNLEYIKAKIDAMNKGVRDYNLQCPSTGLHKWKLTQDNEIDTQIKNVTKNIEPLVIEHLKANKQVAKSSNVSETSLLGLFETRGSGSRQLSESTPNDTQRSYLGTGLWRMLRNALKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GWM6</accession>
    <name>A0A1A0GWM6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_48166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000013">
        <property type="protein sequence ID" value="OBA16133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709239.1">
        <property type="nucleotide sequence ID" value="XM_018858272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GWM6"/>
    <dbReference type="GeneID" id="30031248"/>
    <dbReference type="OrthoDB" id="3699854at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="55" mass="6454" checksum="0361BD2E44B17006" modified="2016-10-05" version="1">MHHHPQNQERALNLSILIVSGPVKLSRRLHSWWCPSVNSFKFQPCDHTPPRTQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ26</accession>
    <name>A0A1A0GZ26_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome quality control complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_61911</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709303.1">
        <property type="nucleotide sequence ID" value="XM_018858320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ26"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ26"/>
    <dbReference type="GeneID" id="30031296"/>
    <dbReference type="OrthoDB" id="207084at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904678">
        <property type="term" value="F:alpha-aminoacyl-tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140708">
        <property type="term" value="P:CAT tailing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043043">
        <property type="term" value="P:peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000003">
        <property type="entry name" value="Zinc knuckle domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="8">
        <location>
            <begin position="630"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="9">
        <location>
            <begin position="937"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="498"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="829"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1039"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="381"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="514"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="833"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA17006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1058" mass="119601" checksum="3DAF985B714B76A3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ31</accession>
    <name>A0A1A0GZ31_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF833-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_48109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709256.1">
        <property type="nucleotide sequence ID" value="XM_018858262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ31"/>
    <dbReference type="GeneID" id="30031238"/>
    <dbReference type="OrthoDB" id="191601at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="SER/THR-RICH PROTEIN T10 IN DGCR REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985:SF8">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="310" mass="34751" checksum="50F7AABD4C30ED87" modified="2016-10-05" version="1">MCILITATDHPDYPFIACSNRDEFFARPTQLATVRDLHTGARILSPLDLGRDEHGTWIGVTSEGRIAILVNYKEPEFVPSKVSRGILPIDYLSSTLSDEEWYKTLDDNLTKSACTGVPVSLKDIGGFTLIYGKLEVDPVLGNLIPLNIMSNRGDRGKIHVTGPDEGGLHGKVARQKHFSVSNSLYYEPWPKVKLGEAKLAELVSTAVEKSYSRDELAEACFQILSTDTYNPTIRYSGDEFSEAKLEELCNSIFIPPLETKSATGTSPEFYGTRTQTVIMFHKSGVIHYYERDLYDSESRVQRVKNQHFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H229</accession>
    <name>A0A1A0H229_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709405.1">
        <property type="nucleotide sequence ID" value="XM_018858202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H229"/>
    <dbReference type="GeneID" id="30031178"/>
    <dbReference type="OrthoDB" id="4088270at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291661" evidence="1">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="50" mass="5551" checksum="5F9D19FBF0D7F3DC" modified="2016-10-05" version="1" fragment="single">QKKIAAPQFRIWSPTTLLSVALCSLTNVDRTGNSASCMVWPQPFCCYDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4P7</accession>
    <name>A0A1A0H4P7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ferrochelatase</fullName>
            <ecNumber evidence="12">4.98.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_47247</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>heme b + 2 H(+) = protoporphyrin IX + Fe(2+)</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.98.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ferrochelatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.98.1.1" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709546.1">
        <property type="nucleotide sequence ID" value="XM_018858139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4P7"/>
    <dbReference type="STRING" id="869754.A0A1A0H4P7"/>
    <dbReference type="GeneID" id="30031115"/>
    <dbReference type="OrthoDB" id="1323at2759"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1400:FF:000003">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108:SF1">
        <property type="entry name" value="FERROCHELATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 12" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 12" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="379" mass="43354" checksum="CBFFFF9001EA5866" modified="2016-10-05" version="1">MDRVLLKCYSTEASKSPTGVVFMNMGGPNKSSETYDFLNRLFSDKDLIPLGPFQTFTAKFIARQRTGSVAEKYDEIGGGSPIRYWSEYQSEKVCKRLDEISPETAPHKPYVAFRYAKPLTEDMLEQMKKDGVTRAVAFSQYPQWSASTSSSSMHELWRQTQIVDPERSIKWSMIDRWPKDKCLVNPFAKLIQEKINEFPAEDREKVVILFSAHSLPMQIVNRGDSYPAEVAASVYAVMEKLNFSNPYRLVWQSKVGPKPWLGAQTDKVVMKLELSENIKGIVLVPIAFTSDHIETLHELDIELQEEVKNPDMIKRAASLNDNEEFISGLADLVKNHLESGQLYSKQLELDWELSKQWTSKTFSHPRELFGDHSKDAKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Y8</accession>
    <name>A0A1A0H4Y8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CBS-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47561</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709636.1">
        <property type="nucleotide sequence ID" value="XM_018858189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Y8"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Y8"/>
    <dbReference type="GeneID" id="30031165"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17781">
        <property type="entry name" value="CBS_pair_MUG70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17782">
        <property type="entry name" value="CBS_pair_MUG70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF26">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN DDB_G0289609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="565"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="58"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="125"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="231"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="297"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="6">
        <location>
            <begin position="437"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="14"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="588" mass="64932" checksum="1BA26904E35872DF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Z2</accession>
    <name>A0A1A0H4Z2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pre-mRNA-splicing factor SLT11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_47483</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing. Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLT11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709528.1">
        <property type="nucleotide sequence ID" value="XM_018858172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Z2"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Z2"/>
    <dbReference type="GeneID" id="30031148"/>
    <dbReference type="OrthoDB" id="10259600at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071006">
        <property type="term" value="C:U2-type catalytic step 1 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12265">
        <property type="entry name" value="RRM_SLT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039171">
        <property type="entry name" value="Cwc2/Slt11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034356">
        <property type="entry name" value="Slt11_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048995">
        <property type="entry name" value="STL11/RBM22-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14089:SF6">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR RBM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21369">
        <property type="entry name" value="STL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="185"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="297"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA18993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="37453" checksum="C6B23362253361E9" modified="2016-10-05" version="1">MSEVPVLCNQCLGLNGHVRMIRQLLGEQCKICTRPFTVFRWSLAGEHRQLKKTILCTTCAQARNCCQSCMVDIDHGIPLDIRDAALKMAGVENPYAVESLSRNSEVNAIMADKLEHQSKGIRANTEEKRENAKKILGALSSKLSGRSVSRPKQKELEVSNKDLSKTISKLPFGGSIDSPEDTTIKSFFVFGFSADTPQYSITSFFEKFGSLDLVTIIHQARCGYVSFAKRSSAEACAQHLKTNGHHKGSKTAALLLLDKKEPVRVSWGAPKNLGNSKEEQNKIAIVVNKVMKQLADKDVAGGRGGRRRDSAKSSSKCGTPLKVTGPTVKKSYKSMSEDFEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H552</accession>
    <name>A0A1A0H552_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TEA-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_47273</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TEC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709615.1">
        <property type="nucleotide sequence ID" value="XM_018858140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H552"/>
    <dbReference type="STRING" id="869754.A0A1A0H552"/>
    <dbReference type="GeneID" id="30031116"/>
    <dbReference type="OrthoDB" id="10006572at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.40">
        <property type="entry name" value="TEA/ATTS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000818">
        <property type="entry name" value="TEA/ATTS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038096">
        <property type="entry name" value="TEA/ATTS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050937">
        <property type="entry name" value="TEC1_TEAD_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834:SF0">
        <property type="entry name" value="PROTEIN SCALLOPED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11834">
        <property type="entry name" value="TRANSCRIPTIONAL ENHANCER FACTOR TEF RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01285">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00065">
        <property type="entry name" value="TEADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00426">
        <property type="entry name" value="TEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51088">
        <property type="entry name" value="TEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TEA" evidence="8">
        <location>
            <begin position="110"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="TEA" evidence="6">
        <location>
            <begin position="110"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="40"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="592" mass="65663" checksum="E2FDBE156AB7A40D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B6</accession>
    <name>A0A1A0H5B6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="12">DHBP synthase</shortName>
            <ecNumber evidence="3 12">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_47383</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709648.1">
        <property type="nucleotide sequence ID" value="XM_018858159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5B6"/>
    <dbReference type="GeneID" id="30031135"/>
    <dbReference type="OrthoDB" id="60371at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000002">
        <property type="entry name" value="3,4-dihydroxy-2-butanone 4-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF18">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="202" mass="22211" checksum="0F1A1278886113D3" modified="2016-10-05" version="1">MSKFSTVPDAIEAFKNGEFLVVMDDESRENEGDLIIAAEKITQEQMAFLVRHSSGYVCCPLSTERANELDLQPMLKDRTDRHGTAYTVTCDFANGTTTGISAHDRALTARLLAASTSKPDDFIRPGHILPLRAVPGLLKSRRGHTEASVQLCELAGLAPAAVICELVRDEDGLMMRLDDCEKFAEKFGIKLVTIDQIVNFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5F1</accession>
    <name>A0A1A0H5F1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DUF125-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_219790</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709702.1">
        <property type="nucleotide sequence ID" value="XM_018855124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5F1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5F1"/>
    <dbReference type="GeneID" id="30028100"/>
    <dbReference type="OrthoDB" id="73465at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990461">
        <property type="term" value="P:detoxification of iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02435">
        <property type="entry name" value="CCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="303" mass="32495" checksum="D88424B6E60FB3BF" modified="2016-10-05" version="1">MLVQVKNVVALLLTKADKSDDDLRHAERLLDENRTPYGAAGGNDRAQNAAAEEAIVLAAKNINSDSEDENEGSGFLNSFDPRVMSDIIIGLSDGLTVPFALTAGLSSLGSSKLVITGGMAELVSGAISMGLGGYLAAKSELDYYHSEVKKEKLQFFKKPESINQEAAEIMFELGASEQTIASFLKDMGHTPKNLIDFVIRFGKGLEEPAEGREFTSALTIGSGYFIGGFIPLLPYFFCDVVRTGLIVSVIVMIVTLFIFGYVKTSVSLGDDCGTKKKVYEGLQMVAIGSVAAGAAWTLVYFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5K9</accession>
    <name>A0A1A0H5K9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_13669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709911.1">
        <property type="nucleotide sequence ID" value="XM_018854405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5K9"/>
    <dbReference type="STRING" id="869754.A0A1A0H5K9"/>
    <dbReference type="GeneID" id="30027381"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052400">
        <property type="entry name" value="Zn2-C6_fungal_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47657:SF7">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN ECM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="2">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="464" mass="50060" checksum="C41B1E1AA0F486C5" modified="2016-10-05" version="1">MDVFSSALKNPLPPARKRSDQQKSELAETLNSLSEPSPYEPTSRTVSTYAPNGQTASQYGDQSQARDSKKKNLRRRHRNSHLGCGICKKRRIKCDEHLPQCFNCLKGRLHCAYLNLDAPAQTALRLAQQNQSLRDEREEELSPKQEEKPRPYKDAPVGPRLHPPLYPSVISPPTDPVPAYANNPPTASSSGASAANNPVPPKSMGPPVPMQAPLSMMHSMYVPVYQLQQMSSAMPYPQPPMQVMPGQQAQTIMYLPEQMPIQYASQHISGTTAPGMSAPSYACYPAPAHSFNHQYEGHPSSGYMMPMQGQYGLSAPRPESGAYYAYSAQTPEVMVSAPKSGILAISVPSQLVLVTHDSPSSSVTSVTGDKADALSATSLTRVVGHPAESSIPLASLLPASLGPSPLLPSMIGVPEISKNASIKLAPIGGKHDASRHDPSKHDPSETESVTKETEKISSIKMLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5P0</accession>
    <name>A0A1A0H5P0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709754.1">
        <property type="nucleotide sequence ID" value="XM_018856294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5P0"/>
    <dbReference type="STRING" id="869754.A0A1A0H5P0"/>
    <dbReference type="GeneID" id="30029270"/>
    <dbReference type="OrthoDB" id="3980854at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="440"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="478" mass="52158" checksum="A564C79D108E5CFB" modified="2016-10-05" version="1">MSPPSMLGIRDLLNEGSSPELVSHSSDDGSRFTSHVGSEPKLEVNSCNHRIDKLYRTRVHPAFLKHHRLLDIALRRKLAPKRTPSAANCGLKLHRKLLEAGLRVRLISAPSIYEIYYQLTPLTSHSKQLYRILADKTQYTLRAEDVSLDLVSMRSGPGGPRQSGLVKTETGSVKTETGPAKTEPGPGSPNSSPPSAHAGPPAEMLQPGKLTARTVRLIDSNLVNLAGVPSKVLKSLTYDKSTTELGGSVSDRPEELVRTFNNLDTAVSSMFAAQKYSLVRVTKCAASNSEASALVKLETAQPGDPSLIDDGEFIEDMVASLGQPDLVPSNLFIKKVIARPRYKVDMKLYFVPTGTPMLLYVVSRAFERDLINGIIDIQSTPDRHICTTFDPAPILDTVRRLVWQSTGYRAAFDEHVPNHITENLHKYFMDLPAEQDSEDSSQTSSTPASRSSMFSLSNDSIDSSGFSLISSASSVAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H666</accession>
    <name>A0A1A0H666_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_13213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dpy-30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709978.1">
        <property type="nucleotide sequence ID" value="XM_018854320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H666"/>
    <dbReference type="STRING" id="869754.A0A1A0H666"/>
    <dbReference type="GeneID" id="30027296"/>
    <dbReference type="OrthoDB" id="417678at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd22965">
        <property type="entry name" value="DD_DPY30_SDC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007858">
        <property type="entry name" value="Dpy-30_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049629">
        <property type="entry name" value="DPY30_SDC1_DD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05186">
        <property type="entry name" value="Dpy-30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="123" mass="13294" checksum="EE555276E3FF0120" modified="2016-10-05" version="1">MEATPSLGDETTPSTIKTEPLAGPQNQHEPSKTDEPVAPEQKNETPDPSGPPAKKAKTEILASAREPPVHEMVGGSSVRQYLNKHLTEHLLEGLKDVSAKKPEDPLLALGEFLVQRSKELNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H699</accession>
    <name>A0A1A0H699_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710013.1">
        <property type="nucleotide sequence ID" value="XM_018858029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H699"/>
    <dbReference type="GeneID" id="30031005"/>
    <dbReference type="OrthoDB" id="4091843at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="313" mass="35045" checksum="49C813D79F9570CB" modified="2016-10-05" version="1">MSQQEVIDLAIASYLKTNDNTARQYLKLRQEVLETTQLVIKEIKESGAVSPETLLQLEKLKFENAKTSLVYKAYIDNITSNLENESTLGGQISVELNERFDDLLQKKKHYDEKTSQLRGELKPVVEKMRLLASNFEAGIGLGSSGSRLEKSERRLFTSKGSKADDKSVFLDYTSAVFSKSELEAQFVDVESKLAALKTVDEISNEDADTKELSTEKGPSIELSTYLNELLQSGRAYTRINDSTYHFDVTKSTSADLGNSRSVISDYENANELLAKEIQGLVARGTEAKERWISNAQKIEMIQALLQEDMDIDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6F4</accession>
    <name>A0A1A0H6F4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_18175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710197.1">
        <property type="nucleotide sequence ID" value="XM_018854773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6F4"/>
    <dbReference type="GeneID" id="30027749"/>
    <dbReference type="OrthoDB" id="4084695at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="169" mass="18665" checksum="CB43B4C403480C0E" modified="2016-10-05" version="1" fragment="single">ARAFSSVGRVLAKNDASTIDSFKLPSQTSINEWEFKYDLMPKVAGPKIPPVSPEAVKQDIARTKKEQIEQELLNRELHASFKVEANHASVLHGGEPVSAEPLYIHERGLSPVDASTPTLRKKLKIANTHKYVQLSLNPEINNPAVVNLGHENKVHHKTATVEAPPEVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6F5</accession>
    <name>A0A1A0H6F5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysophospholipase NTE1</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Intracellular phospholipase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Lysophospholipase nte1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Neuropathy target esterase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_79508</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Intracellular phospholipase B that catalyzes the double deacylation of phosphatidylcholine (PC) to glycerophosphocholine (GroPCho). Plays an important role in membrane lipid homeostasis. Responsible for the rapid PC turnover in response to inositol, elevated temperatures, or when choline is present in the growth medium.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710015.1">
        <property type="nucleotide sequence ID" value="XM_018859077.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H6F5"/>
    <dbReference type="GeneID" id="30032053"/>
    <dbReference type="OrthoDB" id="421051at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034638">
        <property type="term" value="P:phosphatidylcholine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071071">
        <property type="term" value="P:regulation of phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000013">
        <property type="entry name" value="Lysophospholipase NTE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056556">
        <property type="entry name" value="NTE1_P-loop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF29">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24179">
        <property type="entry name" value="NTE_Ploop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 18" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="12 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="32"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="598"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="20">
        <location>
            <begin position="708"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="21">
        <location>
            <begin position="1125"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="18">
        <location>
            <begin position="1129"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="18">
        <location>
            <begin position="1156"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="18">
        <location>
            <begin position="1276"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="18">
        <location>
            <position position="1158"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="1276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA19487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1439" mass="159568" checksum="894E66B366975A7F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6G7</accession>
    <name>A0A1A0H6G7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_23652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710154.1">
        <property type="nucleotide sequence ID" value="XM_018855265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6G7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6G7"/>
    <dbReference type="GeneID" id="30028241"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14369">
        <property type="entry name" value="CUE_VPS9_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041545">
        <property type="entry name" value="DUF5601"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041804">
        <property type="entry name" value="Vps9_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18151">
        <property type="entry name" value="DUF5601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="1">
        <location>
            <begin position="124"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="412" mass="47432" checksum="EA2B35D19FE55B02" modified="2016-10-05" version="1" fragment="single">IVNASEQHQQSHRPFDFQTFLSHLKKKSADPIVRYIRSFLMSFARQTSAMSATQMSKAIRNFKLFIEGKFRVYEPFASMDETDLENSVEGVEKLIMNRLYEFCFPPEATKKFEFHSPSAMSQDLSDDREFSAQIEKFSWILGVHLDVNLDDLSERKRRASKDDVDYMEYAARELNKINDYRAPRDKIICILNACKILFSLLRITDQETNADSFIPLLILVILRSKTKNIICNLHYIERFRGEKWLNHGETSYYLSTVEGAITFIQNIKREDLTIDQTHYDAHMEAWEAELRQKSPKQTIPKKEILESSSEVASRQKLGILPSNVLMASAEIFTKSISNLISNPEEAVSASRPTEEARTSQEQVGFTDNQVGETFNQLCEIFPSLDKDIMRDVVIMNGADFEVSLDVCLQLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S9</accession>
    <name>A0A1A0H6S9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33261</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710141.1">
        <property type="nucleotide sequence ID" value="XM_018856256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S9"/>
    <dbReference type="GeneID" id="30029232"/>
    <dbReference type="OrthoDB" id="333486at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="5">
        <location>
            <begin position="210"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="28"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="173"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="313" mass="34142" checksum="19AC2CE90D0003B1" modified="2016-10-05" version="1">MLRGRLLNTTPFLRCTLRESTKRFMRTVSAPSKQSPAADFGRSNSHLPADLPHTKLDGRSWTKHQLDPILAAKTQTQTPAQLPGLQASRWAKHSLDTPAAAPTRSPTSLPIIQRPGEISLLAPVRRSRTSRFKGFKAATSASPTAQESEPQQNASVDEVKTAPVAELPKVITDPAASATTASVPAPQDSTTATTKAKKPRRQLKPRKALITLTPNALENLRALLDQPEPQLIRIGVKNRGCSGLTYHLEYVSEPGKFDETVEQDGVRVVIDSKALFSIVGSEMDWIDDKLSTRFIFRNPNSKGTCGCGESFMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6U4</accession>
    <name>A0A1A0H6U4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710276.1">
        <property type="nucleotide sequence ID" value="XM_018856134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6U4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6U4"/>
    <dbReference type="GeneID" id="30029110"/>
    <dbReference type="OrthoDB" id="3366546at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050656">
        <property type="entry name" value="PINX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149">
        <property type="entry name" value="G PATCH DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23149:SF26">
        <property type="entry name" value="PROTEIN TMA23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="24066" checksum="3917F3371E95E406" modified="2016-10-05" version="1">MNASSYLQSFGWKEGEALKQGGLRKPILVKHKKDTKGLGSEGNDADMWWERLFDGQLKNLEVSSGENGISVSQNEVEVANHMRKVNLPLYKMFVRGEGLAGTVGKTENAHVKSISIEATAVLDSVEKSLNILSVESKGKKESKKKEKKEKKEKKEKKEKKEKKEKKEKKEKKEKKEKKENTKNCLSDLEHGKLSRKRTLQESKRKLKRSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6V5</accession>
    <name>A0A1A0H6V5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II holoenzyme cyclin-like subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_79641</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex. The SRB8-11 complex phosphorylates the C-terminal domain (CTD) of the largest subunit of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SRB8-11 complex, a regulatory module of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin C subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710170.1">
        <property type="nucleotide sequence ID" value="XM_018859102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6V5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6V5"/>
    <dbReference type="GeneID" id="30032078"/>
    <dbReference type="OrthoDB" id="10266018at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990508">
        <property type="term" value="C:CKM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000411">
        <property type="term" value="P:positive regulation of transcription by galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20513">
        <property type="entry name" value="CYCLIN_CCNC_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000077">
        <property type="entry name" value="RNA polymerase II holoenzyme cyclin-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 12" id="KW-0195">Cyclin</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Cyclin-like" evidence="13">
        <location>
            <begin position="59"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="332" mass="38903" checksum="ADFA070450738FAF" modified="2016-10-05" version="1">MSADYWSSTQRTNWQVTREQLVENRRRLALLEKKMIQSGLIKDYPHINCDVNMKIYLHNLIVKLGRRLNVRQVALSSSEIYLSRFLTRVSIKEVNVYLLVTTCLYVACKMEECPQHIRLIVSEARNLWPEYIPQDVSKLAEFEFYLIEEMDMYLVLHHPYRSLLQVRDFLRENASVYGFTLSNVELQHSWSLINDSYITDLHLIFPPHIIAMAAIYITIILEKRLNSIRAQGSLGIDAEGKQGSMHIEDLMSLTATNRESDMGLQPGDNLESQRLDEDTLKIERFMNFFNHSHVNLDEVVEAMQEMIGLYFAWNNYNESLVRKALQYMLLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H756</accession>
    <name>A0A1A0H756_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_88235</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710257.1">
        <property type="nucleotide sequence ID" value="XM_018859289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H756"/>
    <dbReference type="STRING" id="869754.A0A1A0H756"/>
    <dbReference type="GeneID" id="30032264"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051630">
        <property type="entry name" value="Corepressor-Demethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017:SF1">
        <property type="entry name" value="LD02225P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14017">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="477"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="599" mass="64834" checksum="581F583F706F5509" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H772</accession>
    <name>A0A1A0H772_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional activator leucine zipper</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_45216</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710401.1">
        <property type="nucleotide sequence ID" value="XM_018857891.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H772"/>
    <dbReference type="GeneID" id="30030867"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005933">
        <property type="term" value="C:cellular bud"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007118">
        <property type="term" value="P:budding cell apical bud growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025614">
        <property type="entry name" value="Cell_morpho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025481">
        <property type="entry name" value="Cell_Morphogen_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029473">
        <property type="entry name" value="MOR2-PAG1_mid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF30">
        <property type="entry name" value="PROTEIN FURRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14225">
        <property type="entry name" value="MOR2-PAG1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14228">
        <property type="entry name" value="MOR2-PAG1_mid"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14222">
        <property type="entry name" value="MOR2-PAG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cell morphogenesis protein N-terminal" evidence="2">
        <location>
            <begin position="248"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1094"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1406"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis central region" evidence="4">
        <location>
            <begin position="1727"/>
            <end position="1900"/>
        </location>
    </feature>
    <feature type="domain" description="Cell morphogenesis protein C-terminal" evidence="3">
        <location>
            <begin position="1930"/>
            <end position="2181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="959"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2431"/>
            <end position="2462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="2431"/>
            <end position="2443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2482" mass="279459" checksum="887AC9534BA63C6D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H784</accession>
    <name>A0A1A0H784_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TAFII55 protein conserved region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710282.1">
        <property type="nucleotide sequence ID" value="XM_018859005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H784"/>
    <dbReference type="STRING" id="869754.A0A1A0H784"/>
    <dbReference type="GeneID" id="30031981"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08047">
        <property type="entry name" value="TAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037817">
        <property type="entry name" value="TAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006751">
        <property type="entry name" value="TAFII55_prot_cons_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF0">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII55 protein conserved region" evidence="7">
        <location>
            <begin position="139"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="505"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="407"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="576"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="599" mass="66927" checksum="0C9B0E3340F818D8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H788</accession>
    <name>A0A1A0H788_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_45091</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710484.1">
        <property type="nucleotide sequence ID" value="XM_018857875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H788"/>
    <dbReference type="STRING" id="869754.A0A1A0H788"/>
    <dbReference type="GeneID" id="30030851"/>
    <dbReference type="OrthoDB" id="423462at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd12287">
        <property type="entry name" value="RRM_U2AF35_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="32"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="9">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="6">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="192"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="27830" checksum="7CD7B678190E04A7" modified="2016-10-05" version="1">MDRSVCSFYQKIGACRHGEKCSRKHIRPSESKTVLLANIYQNPKVSTNENETMNDKQMIEAFDHFYSDVFKKLATVGEVERIVVCENENFHLSGNVYVRFTTAQAANDAVMLLNQEWYAGRPVYCELSPVSSFQEANCRAHDTNSCTRGDHCNFMHVIRPTDDLRVRLRQAQDKQIALTKIRLAMGDPSWGMQWEEPSSEKYKRESVPQATSSVPITSNNLETPGDIGGDPEDSKVEAVAKLFGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7B0</accession>
    <name>A0A1A0H7B0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_14817</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710313.1">
        <property type="nucleotide sequence ID" value="XM_018854543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7B0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7B0"/>
    <dbReference type="GeneID" id="30027519"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16479">
        <property type="entry name" value="RING-H2_synoviolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050731">
        <property type="entry name" value="HRD1_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058051">
        <property type="entry name" value="Znf_RING_synoviolin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22763">
        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="318"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA19788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="402" mass="46418" checksum="DA21228E139026CB" modified="2016-10-05" version="1" fragment="single">LAYSAFSLGSFGASVLLLALNSYDFLTFLQELTDSFRLTILLNFIVFCCLSFGYLSVRVLFHRFRIIEIEHIADQLPFYALNLLFILFNDGNMILNTVLTGLTLLLKVHHIMTYERIDFLQVQVVNRMSQQQFSKARVFASFFLNAHVIYLFLLLPADFVLARFLAYDVFQGIGSMGSLLFGIQFGVLWLDCFAFLGKLILNVYELVFYRCVDVQEDLIEDEDVLEEHIWESRAVYVQGFEIYHSILKTLFYAAFLYTLYFHSRVALPIPLIQGCIVSIHQAFKKVYQLMSFLSHSRFLEDQLACPSEEELVAADYICIICREDMHFPETFAANRNRPLNPRKHPKKLQCGHILHLCCLKDWLERSNSCPLCRKVVFKKAQPVTERNATNPPAPTPVGRPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7E3</accession>
    <name>A0A1A0H7E3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_79269</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710471.1">
        <property type="nucleotide sequence ID" value="XM_018859026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7E3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7E3"/>
    <dbReference type="GeneID" id="30032002"/>
    <dbReference type="OrthoDB" id="5424147at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024461">
        <property type="entry name" value="CCDC90-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360:SF12">
        <property type="entry name" value="MOZ PROTEIN REPRESENTS A CHROMATIN-ASSOCIATED ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14360">
        <property type="entry name" value="PROTEIN FMP32, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07798">
        <property type="entry name" value="CCDC90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="274" mass="31737" checksum="78690C77CE62D39E" modified="2016-10-05" version="1">MSFSSIRLQQVKSDPNDHPLGTVDINKINTAAEPSGENSSPTQSSDDFLTTIFEHLEPYIDTYDVYKQLIDAGFTLSQSDEILNLVIVQLNSKLTKLLTIYSQLYELENERYLFESAQQELHVDITRNREQHINDAINQINALERDYGIISDELNNHFIQMKNDIQVAINDQKSENTLMSKKIMLRMQETNHKITTELNMAMRLEIESLRWHLSRWGLIAILISIITACAGFYAYRVHHQKQLTEKNEFVPLVIYEPSEFDEDDYHADLDPNAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7X1</accession>
    <name>A0A1A0H7X1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CENP-T/Histone H4 histone fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_44587</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710646.1">
        <property type="nucleotide sequence ID" value="XM_018857812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7X1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7X1"/>
    <dbReference type="GeneID" id="30030788"/>
    <dbReference type="OrthoDB" id="4077024at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035425">
        <property type="entry name" value="CENP-T/H4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15511">
        <property type="entry name" value="CENP-T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-T/Histone H4 histone fold" evidence="5">
        <location>
            <begin position="466"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF15511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="572" mass="64714" checksum="DDD02ED09416603F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7X9</accession>
    <name>A0A1A0H7X9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">cAMP-dependent protein kinase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_212604</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">Tetramer, composed of 2 regulatory (R) and 2 catalytic (C) subunits. In the presence of cAMP it dissociates into 2 active monomeric C subunits and an R dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710615.1">
        <property type="nucleotide sequence ID" value="XM_018855057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7X9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7X9"/>
    <dbReference type="GeneID" id="30028033"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046580">
        <property type="term" value="P:negative regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046827">
        <property type="term" value="P:positive regulation of protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097271">
        <property type="term" value="P:protein localization to bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010603">
        <property type="term" value="P:regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12098">
        <property type="entry name" value="DD_R_ScPKA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000039">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 8 9" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="203"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="11">
        <location>
            <begin position="321"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="69"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="435" mass="47605" checksum="97B110A521D88FEC" modified="2016-10-05" version="1">MSSYQFEELNQLQREIIAENPADILQFSANFFSNKLALQRAHAWQSQARAKAAGIELFPNTSSVSPGIMGSFKTPTFRQPSFKSPFDDNDPHSDHAKDHHDDGSSKKNESVGGLFKNNFGQSLGSKESVHPETDPAESSAGDKSETKPINKLPRTFNANRRVSVSAEAMNPDEFKSDSWKPPVNDLNPQQRGELGKTLSLNFLFQQLDSNSKSTVLSALIKKNFLKETEIIKQGEVGDYFYIIESGVVDFYVNGSKVNTSGDGSSFGELALMYNSPRAATAVAASDVTCWALDRATFRRILLEGTFNRRIMYEDFLKDVKVLLGLTSQERSKLASALSTEIYSAGEEIVTEGEVGEKFYLIESGTCVISKKGEGVIGEIGKGKYFGEVALLNDLPRQATVTAANNVIVATLGKSGFTRLLGPALDVLRKQDPTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8C9</accession>
    <name>A0A1A0H8C9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_12389</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710891.1">
        <property type="nucleotide sequence ID" value="XM_018854222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8C9"/>
    <dbReference type="GeneID" id="30027198"/>
    <dbReference type="OrthoDB" id="5582146at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="374" mass="42361" checksum="BF43C94CA5DF1004" modified="2016-10-05" version="1">MPLIGGERDLETNLALIQILYLSRTETEPTILAPAMENLRTALSLAFIAGKHIAIHTDDYAACLAALRNVVLLSFSADDMVEIDLHLCPTYNSLVSQMVKSREGERPQLYKVVIWKNLEALEATLRVKSDILRVFDEIERYNTLASRDIDKATPIRFGEYLVTIPDMFVIVPVMAAGDRFPRLHRQVKDRIWFCQSFCHVPRQETPASEIPHDKILRARQLLPAVFANAEIQEYVCSLMVFTRSHRLCSLAPLTTRPSMRAAEGILVLAKALVAWASLDDSASDFVTPDFVKIAYRKIGYWIVDWETNQLFQGGGSELRKRTEISVLTGDWYGSEWACVKKYLDKFRAHSDPNSTTGYTNRIVEDVLKSVLPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8J8</accession>
    <name>A0A1A0H8J8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_78662</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710730.1">
        <property type="nucleotide sequence ID" value="XM_018858911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8J8"/>
    <dbReference type="GeneID" id="30031887"/>
    <dbReference type="OrthoDB" id="4021219at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="516" mass="57470" checksum="D9198BBCF81D38E9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H901</accession>
    <name>A0A1A0H901_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_32481</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711002.1">
        <property type="nucleotide sequence ID" value="XM_018856055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H901"/>
    <dbReference type="GeneID" id="30029031"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="125" mass="14303" checksum="6ABDAF4D97BA986C" modified="2016-10-05" version="1">MNRRYKQNEETNRPQINQNPNNQNPNSLSPKETENKRKHSREINRNSKHMKTDPDKRHTWKNRSCAKAPGPPAHVTTTAIVASPGKTRMDRARTEPAEEGPREFGTQTSANTGRGRPQPRNRNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H982</accession>
    <name>A0A1A0H982_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_32538</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711077.1">
        <property type="nucleotide sequence ID" value="XM_018856073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H982"/>
    <dbReference type="STRING" id="869754.A0A1A0H982"/>
    <dbReference type="GeneID" id="30029049"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF38">
        <property type="entry name" value="CHITIN SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="673"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="716"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="751"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="787"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="924"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="955"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="9">
        <location>
            <begin position="251"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1007" mass="115020" checksum="8103A08E9BE3104F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9A9</accession>
    <name>A0A1A0H9A9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">threonine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_203302</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711226.1">
        <property type="nucleotide sequence ID" value="XM_018854961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9A9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9A9"/>
    <dbReference type="GeneID" id="30027937"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070159">
        <property type="term" value="P:mitochondrial threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000039">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF50">
        <property type="entry name" value="THREONINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="52"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA20704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="455" mass="51792" checksum="CABFCC5C54493991" modified="2016-10-05" version="1">MISCRLPLRRAPRMFYSTTTKESSTQANHAIATKQQLFVTDPVTPGLVFFLPHGTRVFNKLVGFMKNQQLRYGFHEVITPLIYKTQLWEKLGHWENYKDDMFKVVGNDTLKDAAENGAGPGAGHVHEYGLKPMNCPGHCLIYAKFDRSYNELPIRYSDFLSLHRNEASGALSGLTRVRRFHQDDGHIFCEVHQIHQEITNTINMIKDTYAVFGIDSERDVDFFLSTRPDKYMGDLATWDNAEAQLTQVLNGCTHQWQVREGDGAFYGPKIDVLLTDAFGKKHQVGTIQLDFQLPARFELKYTLASGSRENQPIMVHRAVFGSLERFFAILLDHYQGKWPFWINPRQAVVVPVAERHHEAAQQVQKQLSGEILASDSTVAPLTGYNFHVDVDKRSETVGTRIKDALQKGYSYIIMIGDKDLASGTISVRTRDDRKVENVAVAELYLRFVALEKAYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9B2</accession>
    <name>A0A1A0H9B2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoA-dependent acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711107.1">
        <property type="nucleotide sequence ID" value="XM_018856084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9B2"/>
    <dbReference type="GeneID" id="30029060"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="75" mass="8496" checksum="700ED9EFBD031351" modified="2016-10-05" version="1">MLNDDGSSPLELTSLSFQDNHAKRIALWKIIYYKNTGWLTFHSSHCFTDGGSVLAYLKDFVEGLNQSQESIAREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9G9</accession>
    <name>A0A1A0H9G9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Root hair defective 3 GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SEY1</name>
        <name evidence="12" type="ORF">METBIDRAFT_43702</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="8">Enriched in the cortical ER. Concentrated in punctae along the ER tubules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711291.1">
        <property type="nucleotide sequence ID" value="XM_018857688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9G9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9G9"/>
    <dbReference type="GeneID" id="30030664"/>
    <dbReference type="OrthoDB" id="1597724at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016320">
        <property type="term" value="P:endoplasmic reticulum membrane fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01851">
        <property type="entry name" value="GBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000727">
        <property type="entry name" value="Protein SEY1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03109">
        <property type="entry name" value="Sey1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030386">
        <property type="entry name" value="G_GB1_RHD3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008803">
        <property type="entry name" value="RHD3/Sey1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046758">
        <property type="entry name" value="Sey1/RHD3-like_3HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45923:SF2">
        <property type="entry name" value="PROTEIN SEY1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05879">
        <property type="entry name" value="RHD3_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20428">
        <property type="entry name" value="Sey1_3HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51715">
        <property type="entry name" value="G_GB1_RHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="757"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="topological domain" description="Lumenal" evidence="8">
        <location>
            <begin position="778"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="780"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="802"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="GB1/RHD3-type G" evidence="11">
        <location>
            <begin position="78"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="839"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="839"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="88"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="862" mass="95943" checksum="6F5EAF3B823E58A6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9I6</accession>
    <name>A0A1A0H9I6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_78972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711177.1">
        <property type="nucleotide sequence ID" value="XM_018858980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9I6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9I6"/>
    <dbReference type="GeneID" id="30031956"/>
    <dbReference type="OrthoDB" id="4383808at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="63" mass="7248" checksum="787C7927805453D3" modified="2016-10-05" version="1">MYCLGEFIRPVSCYTLLSGFRLPWPSSGCLDELTPFVVSDERVFWHFNFTFGSSRIASSAYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9L1</accession>
    <name>A0A1A0H9L1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Bystin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_43933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bystin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711336.1">
        <property type="nucleotide sequence ID" value="XM_018857719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9L1"/>
    <dbReference type="STRING" id="869754.A0A1A0H9L1"/>
    <dbReference type="GeneID" id="30030695"/>
    <dbReference type="OrthoDB" id="2192561at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007955">
        <property type="entry name" value="Bystin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12821:SF0">
        <property type="entry name" value="BYSTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05291">
        <property type="entry name" value="Bystin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="444" mass="50279" checksum="451E98DB94FA4C0D" modified="2016-10-05" version="1">MGKSAPDAKDRIRHNPLHKDITSSGGRLREQPRGAKRAARELPEEGYLDAATSRKILQLAKEQQEEMDREEMVVEESAAPVFAASAALDDQDDSDSEEYSDLEGEFYEEEEIAVDEKDQELFDKYFGTGAPTVNLADKILAKIQEKELSKGPHQPASEQQQNAVKLPPKVIAAYEKIGSILATYKHGKLPKLFKVLPTLKNWEDVLYVTNPQSWSSHATYEATKLFVSNLQANQAQKFVENVLLEKFRVSIEDSETHSLDYHLYRAIKKSLYKPGAFFKGFLLPLVDGHCTVREATIVASVLAKVSVPVLHSSVALAQLVQREFRPASTVFIRVLIEKKYALPYQTLDELVFYFMRFRNAAQAEMSDGNDASMTDLPVVWHKAFLAFAQRYKNDITDDQRDFLLETVRQRFHHAIGPEIRRELLSGVPRMDAPQAKEATMEEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA87</accession>
    <name>A0A1A0HA87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sorting nexin-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Autophagy-related protein 24</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_44352</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sorting nexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711310.1">
        <property type="nucleotide sequence ID" value="XM_018857782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA87"/>
    <dbReference type="STRING" id="869754.A0A1A0HA87"/>
    <dbReference type="GeneID" id="30030758"/>
    <dbReference type="OrthoDB" id="205639at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF2">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="12">
        <location>
            <begin position="143"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="325"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="532"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="620" mass="69780" checksum="498F5523523B47E6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA96</accession>
    <name>A0A1A0HA96_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_42038</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711446.1">
        <property type="nucleotide sequence ID" value="XM_018857486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA96"/>
    <dbReference type="STRING" id="869754.A0A1A0HA96"/>
    <dbReference type="GeneID" id="30030462"/>
    <dbReference type="OrthoDB" id="75343at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA20936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="183" mass="19302" checksum="BCDA7D5328453774" modified="2016-10-05" version="1">MVFGVYTGPQEPEKPLALMTQEEQAEMGAKKMVEAMQSCPGKTVMAGGSGFFLGGFFGLFMASMAYDVPVGGSGAGHISELPFKQQMKLQFSDMAKRSYSSAKNFGYIGMIFSGVECSIESLRAKHDIYNGVSAGCITGAGLSIKAGPQAALLGCAGFAAFSTAIDLYMNSEHAAPPRNDYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAC6</accession>
    <name>A0A1A0HAC6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycerate mutase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_11562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711476.1">
        <property type="nucleotide sequence ID" value="XM_018854017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAC6"/>
    <dbReference type="GeneID" id="30026993"/>
    <dbReference type="OrthoDB" id="496981at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07040">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050275">
        <property type="entry name" value="PGM_Phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100">
        <property type="entry name" value="BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48100:SF1">
        <property type="entry name" value="HISTIDINE PHOSPHATASE FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="309" mass="35557" checksum="5846417316207575" modified="2016-10-05" version="1">MSTKPDAIDVHNSSINSEDREQYACAQSLETGPRWKFEIVPGCFKQLLPETDETTFDYFKEGFGKLKPWPQLASELRELNQNSPQHVAYKLLLLGRHGQGFHNLANLIYGDEQWNAKWLHLTTDGKIVWGPDPELTDLGHSQAKDNHKQLQAEIQQGLHLPTRWFSSPFRRSLDTLIGTWEGHVDLQKAQPFIMEDLRETLGVHTCDKRSPRRVIVERYLDRGFIIEDGFAEEDELFKDDYREKVWEQAIRQERAFQHIFSTTDKHTDDFISVTSHSGSIRTQLLVLGHRAFAIGTGGMIPVVVKATYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAJ0</accession>
    <name>A0A1A0HAJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gfd2/YDR514C-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711402.1">
        <property type="nucleotide sequence ID" value="XM_018857487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAJ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HAJ0"/>
    <dbReference type="GeneID" id="30030463"/>
    <dbReference type="OrthoDB" id="5953249at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040151">
        <property type="entry name" value="Gfd2/YDR514C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048519">
        <property type="entry name" value="Gfd2/YDR514C-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28083:SF1">
        <property type="entry name" value="GOOD FOR FULL DBP5 ACTIVITY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21762">
        <property type="entry name" value="DEDDh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfd2/YDR514C-like C-terminal" evidence="1">
        <location>
            <begin position="57"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="250" mass="29453" checksum="D3381A1075D5E3F4" modified="2016-10-05" version="1" fragment="single">MLRIQVRANSTASIVQAQLAARLETYFERTTFKFLKPQHVDELRKAMELVYLRKRPLVSIDVEAYERKQKLITELGVAIYDPENQWLLMQPHVRTLHIISDENRRFLNSQFVPNHKYYFNGSTSYQMKMKLLLKFLKETFRYFFEDRGAVMVGHHLSGDIKWVKSHGVKMADDVSHIDTQKLFHLSRRRGATLRGILHTVDIPHAKLHNAANDAYYTLLAAMRYCDPECRQHYNLDTFVDQPKPTDEEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAP2</accession>
    <name>A0A1A0HAP2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_173349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711580.1">
        <property type="nucleotide sequence ID" value="XM_018854708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAP2"/>
    <dbReference type="GeneID" id="30027684"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="343" mass="37468" checksum="6B5F489702130CC3" modified="2016-10-05" version="1">MTSSARRPERRRSRMASLPGGPRQTHGGAVPVACCARPSARTVLESSPGVPAGRSQGGLGAVARGTGGFGEAGRFCMGVRGTRCPVRPTVWERGLLLPFFLERHSRESPVRDCSIEERMASKAGLPTAAWCSGCQQVHGRMVLPTQRKQGGIFHLRNACQAGTHRDEHTETYPGRNGATQDTRKTEHVLTARVGPMAWRAYAWSSLASRMRFVCLMCSSLLCSPAPVILLSLAILLLDCTMRFSHEDAGSHIAWTADYCLKMGVFWSCTIFQTQLVCYPLDAVLLEPTSMYCISHCQAVGALVPLPDVHMFSFSFSALARDINTFSFSFSASARDINTFSFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAQ5</accession>
    <name>A0A1A0HAQ5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_173929</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711606.1">
        <property type="nucleotide sequence ID" value="XM_018854711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAQ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HAQ5"/>
    <dbReference type="GeneID" id="30027687"/>
    <dbReference type="OrthoDB" id="338531at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052406">
        <property type="entry name" value="Chromatin_Remodeling_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970:SF14">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="5">
        <location>
            <begin position="408"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="640" mass="69878" checksum="1D4AD4266A83E26C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB04</accession>
    <name>A0A1A0HB04_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_11732</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ATP = O-phospho-L-seryl-[pyruvate dehydrogenase E1 alpha subunit] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23052"/>
            <dbReference type="Rhea" id="RHEA-COMP:13689"/>
            <dbReference type="Rhea" id="RHEA-COMP:13690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711680.1">
        <property type="nucleotide sequence ID" value="XM_018854073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB04"/>
    <dbReference type="STRING" id="869754.A0A1A0HB04"/>
    <dbReference type="GeneID" id="30027049"/>
    <dbReference type="OrthoDB" id="241648at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16929">
        <property type="entry name" value="HATPase_PDK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000065">
        <property type="entry name" value="[Pyruvate dehydrogenase (Acetyl-transferring)] kinase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF3">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="10">
        <location>
            <begin position="353"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="512" mass="57486" checksum="330DE3265D482A93" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB82</accession>
    <name>A0A1A0HB82_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_182134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711899.1">
        <property type="nucleotide sequence ID" value="XM_018854781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB82"/>
    <dbReference type="GeneID" id="30027757"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="242" mass="25855" checksum="2DDE5A67946EF9CF" modified="2016-10-05" version="1">MALAPQALAPTAQGNKARAGPNRTHPLVAGRLFGAKTGKDKMGKRYKKNAEWDKQALSSRRFFFAAPDCSQAVNCCGCLGDLCGDSPLVFGGRKEILVDFLGGFPDSLKRGPQFLKTLHFAHCGLGLVGFLCLVALALNSRLLRTGGSVDMVELGVDSAVRTAAGLALYSRYCLGPDLGPGSKSPGARPDFAVFRELRNGERMGNALAASHRNKPLETQRPVFSETWLRQGSGENDANLAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBK1</accession>
    <name>A0A1A0HBK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_41711</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMD3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712024.1">
        <property type="nucleotide sequence ID" value="XM_018857443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBK1"/>
    <dbReference type="GeneID" id="30030419"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048898">
        <property type="entry name" value="OB_NMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21192">
        <property type="entry name" value="OB_NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="10">
        <location>
            <begin position="20"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="12">
        <location>
            <begin position="252"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 OB-fold" evidence="11">
        <location>
            <begin position="316"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="466"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="477"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="508" mass="58225" checksum="6C81BA42E00EDA5D" modified="2016-10-05" version="1">MSYAPLQPHTHQEGVATVLCCNCGVPMDGSLGLVMCYDCIKLNVDITEGIPREANISFCRNCERFLQPPSQWIRAELESRELLAICLRRLKGLSKVRLIDASFIWTEPHSRRIRVKLTVQGEALQNTIVQQTFEVEYVVIAMQCPDCAKSFTTNTWRATVQIRQKVAHKRTFLYLEQLILRHNAHVDTISIQEAKDGLDFFYAQRNHATKMVDFLSAVAPIKNKRSEELVSIDTQSGSSSYKFTFSIEIAPICRDDLVVLPKKLANQFGNITRLVVCHKVTNMLQFIDPNTLQTADLPAALYWKKPFLPLLDVNQLVEFIVLDVEPTGDMKGKYVLADVTVCRACDLGVNDRTFYVRSHLGAFLHAGDLAMGYYLTNTNFNSDLWDSLDTENTPEVVLIKKHYTRKSKKNRNRKWKLKRMAREHNEIVANDDSRQAKQEQERAERDYELFLQELEEDQELRHTINLYKAGEEPVPSAEDENDMEEDDEDAVEIGIDELLDEIDGMTLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBU9</accession>
    <name>A0A1A0HBU9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cyclin-dependent kinases regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_31898</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CKS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711862.1">
        <property type="nucleotide sequence ID" value="XM_018855942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBU9"/>
    <dbReference type="STRING" id="869754.A0A1A0HBU9"/>
    <dbReference type="GeneID" id="30028918"/>
    <dbReference type="OrthoDB" id="440676at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061575">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.170.10:FF:000002">
        <property type="entry name" value="Cyclin-dependent kinases regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.170.10">
        <property type="entry name" value="Cyclin-dependent kinase, regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000789">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036858">
        <property type="entry name" value="Cyclin-dep_kinase_reg-sub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23415">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT/60S RIBOSOME SUBUNIT BIOGENESIS PROTEIN NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01111">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00296">
        <property type="entry name" value="CYCLINKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01084">
        <property type="entry name" value="CKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55637">
        <property type="entry name" value="Cell cycle regulatory proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00945">
        <property type="entry name" value="CKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU311113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="12374" checksum="8D996DB7364F82E0" modified="2016-10-05" version="1">MSGRPRYLTDTERAKILEFLDMIHYSPRYNDDHCEYRHVMLPKNMLKAIPQDYFNPETGTLRILMEEEWRGLGITQSLGWVHYETHAPEPHILLFKRPLNFDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBW5</accession>
    <name>A0A1A0HBW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PXA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_28445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712139.1">
        <property type="nucleotide sequence ID" value="XM_018855355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBW5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBW5"/>
    <dbReference type="GeneID" id="30028331"/>
    <dbReference type="OrthoDB" id="5582218at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003114">
        <property type="entry name" value="Phox_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02194">
        <property type="entry name" value="PXA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PXA" evidence="1">
        <location>
            <begin position="43"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="503" mass="56473" checksum="547CB506BA2E4242" modified="2016-10-05" version="1" fragment="single">VELPEFQLLVKLYVPANLLIKNNQVSVKQLAGYLPILVDQRFPELNFELHIFLSSLVTAYVLSWYCTKLNTENMAFVLDVYETLCMFIKDMSSRILALVELPALLATINQLAEIVDQHIRDNLVENGIPNYALAILKRHAQNILEETDLPSLRQRYLRENHIIFNHKYGSSVQGQRPLEGNPLLQSANGIDADPTMQQTYLKVLVKNILRTAFSGSGLTKQSPESSVIVFNFLSVLIADLVLEKLLAKLSAPDFLVSQLIGGVSGKLHSKLNTKESPESTVLIELHAHITKAYYEKAYDDLSTALSAFQLCLATLKPSLTILFSPMISLLDAITGFKDKFPNLTSLSGLLRSVLFSIGDLALKLEVFAQTYFMKKLRASLLVEDAFLAHLVESLRCSLFDKVGDTVTDSKMKTPLEVRDEIFDLLRSQLSRKLPLNISIPWCVYTNQREAETKKGIEDILRIFAPLDQTGNEYSGNKSDMNLLLVIKLFDCVVQNIYPELASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBX1</accession>
    <name>A0A1A0HBX1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_185986</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711990.1">
        <property type="nucleotide sequence ID" value="XM_018854800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBX1"/>
    <dbReference type="GeneID" id="30027776"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="199" mass="21953" checksum="5BF6E8597333D9CD" modified="2016-10-05" version="1">MERSARGKAYQGHGFAAKLRSIARPGESRNGIRRSLRVNTGLSPWATAGPMRVPRDWRARLHRMGPLGHSWCAGVGVWHWKRATRLGPGPFSFPVKREERRAKTEFFADVEMTQKQLSISCCLQPFSPGQLLTKGPMFFWLGALLHTSPQPDPHPPARLPHTRELAGVRGSAPICTPHAKAIPGATYRKSGVSPFSGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC00</accession>
    <name>A0A1A0HC00_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="2">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_42806</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Pyridoxal 5'-phosphate (PLP)-binding protein, which may be involved in intracellular homeostatic regulation of pyridoxal 5'-phosphate (PLP), the active form of vitamin B6.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711912.1">
        <property type="nucleotide sequence ID" value="XM_018857589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC00"/>
    <dbReference type="STRING" id="869754.A0A1A0HC00"/>
    <dbReference type="GeneID" id="30030565"/>
    <dbReference type="OrthoDB" id="10264196at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042816">
        <property type="term" value="P:vitamin B6 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd06822">
        <property type="entry name" value="PLPDE_III_YBL036c_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000018">
        <property type="entry name" value="Pyridoxal phosphate homeostasis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00044">
        <property type="entry name" value="YggS family pyridoxal phosphate-dependent enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PROLINE SYNTHETASE CO-TRANSCRIBED BACTERIAL HOMOLOG PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146:SF14">
        <property type="entry name" value="PYRIDOXAL PHOSPHATE HOMEOSTASIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alanine racemase N-terminal" evidence="5">
        <location>
            <begin position="22"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 3">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="246" mass="27228" checksum="63A5DB952BAC10B4" modified="2016-10-05" version="1">MSFPQPTAERQAELIENYEAVWGQVKAHLASLPVRLVAVSKLKPSSDIMGLYNHGVRHFGENYVQELVAKSKELPKDIKWHFIGGLQSGKCKDLGRDIENLYAVETIDALKKCKKLESTRAAAGLSPVNIYLQVNTSDEAQKSGYTLSNLEEVYETVEYVINDCKSLQLQGLMTIGSFAQSTSEEEVNQDFATLVELKLILDKKYSLDLQLSMGMSNDLTTAIRQGSTSVRVGSSIFGARPPRNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC64</accession>
    <name>A0A1A0HC64_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Mis12-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_41770</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mis12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712234.1">
        <property type="nucleotide sequence ID" value="XM_018857448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC64"/>
    <dbReference type="GeneID" id="30030424"/>
    <dbReference type="OrthoDB" id="1884855at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008685">
        <property type="entry name" value="Centromere_Mis12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527:SF2">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05859">
        <property type="entry name" value="Mis12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="298" mass="34024" checksum="F2E546242B3B5B8B" modified="2016-10-05" version="1">MNSSDYRVTALLTEHLGFAPLTLVDEVINAVNKIMYNCTDALENFLVKRREAQLKKLRMHNGIGEDDADIDTDGFMTDQIAAAERNGEVFPYAEIRSGTAELETLLVSHVDKNFDKFELYTLRNILTMPRDLVEDGWVRLKHHEHLDTVDFASVSEDSSARIKSLIRNISMELQLRKLLQFQLAKAKKLVSLLTRYKHSIDSLILTHLDTELSPDSARILKENLTPMNENIYYLLSQLNDLIEQALRLKQTFTKDTALKEGGQVQFWPTNREDYIREKTIKLLENITGTSPGSRPSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC83</accession>
    <name>A0A1A0HC83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable transporter MCH1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_42507</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711992.1">
        <property type="nucleotide sequence ID" value="XM_018857546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC83"/>
    <dbReference type="STRING" id="869754.A0A1A0HC83"/>
    <dbReference type="GeneID" id="30030522"/>
    <dbReference type="OrthoDB" id="199930at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF45">
        <property type="entry name" value="TRANSPORTER MCH1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="373"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="406"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="475" mass="51994" checksum="E6C3E0F2B513A3B9" modified="2016-10-05" version="1">MQKSLSALTYKIKTGLAARYDLVHLKRWAFMIALLSCLSLGSIMLFSLFLLALHHAVGLTYTQINLIVSFSAVGMYLCLPVLGYLADCYGPALLSIISIWAFCPAYFVNAAVVRALENSPSAAADLSRGSVFAMAASFCFIGLATSSLYFSSLLTCAKIYPDHKGLAISLPVSCYGVLSLLGSQILKLDYFHASRDILDLYKVFMFFSVLYFFVGILNLVSNLIVSIEQDVIFNDSEPLLSAMELGPEPALTPQCSAVEPPHHRERYASFLRDKSAWILLVALFFSIGPLECFQNNLGLIIEATAGPSVQLSDQVLVIAALSTAIRLFVGVMIDWVSSEKRRYPVCKVWFLLALMAVGIAGQIMPIFSSRFSVIALLNGICYGGLFTAFPTVVASIWGVDLMGSTWGSFMVAPAFGSITFSLLHGYSMDLCASDSGALGCLDRYFKLTASCFCIGMVLIAVVWRGFWWRRGMKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC84</accession>
    <name>A0A1A0HC84_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42659</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712013.1">
        <property type="nucleotide sequence ID" value="XM_018857570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC84"/>
    <dbReference type="STRING" id="869754.A0A1A0HC84"/>
    <dbReference type="GeneID" id="30030546"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32257" checksum="928FE6C648E61B39" modified="2016-10-05" version="1">MEKFSNWRDKGTGISPFYPVEVKVSAARKYVVNPVILALKWPFFVALYLFASVAPKAATRIIFRSLFSIADIDLLVEGVRKSNANETNAKKPSVGEVVVLNLVSPLDIFVIFLTSNVESLSSLVVVIPWKEDLYTFTAWEALSVLFQPLDSSITQGARLGGDARDMMREKLVVVFAEGSASNNKAILPLQPPCAQLWQSGDPPVKVAVLRYYPNGLSLPVPHMTKAQYVSRLLTLPSKAFVKVKIVPLRAASLKACQAAFVENGLCHVQLGVEEKLKFFEYFQDYSIKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC87</accession>
    <name>A0A1A0HC87_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histone acetyltransferase GCN5</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_23765</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712023.1">
        <property type="nucleotide sequence ID" value="XM_018855268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC87"/>
    <dbReference type="STRING" id="869754.A0A1A0HC87"/>
    <dbReference type="GeneID" id="30028244"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140671">
        <property type="term" value="C:ADA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036408">
        <property type="term" value="F:histone H3K14 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043993">
        <property type="term" value="F:histone H3K18 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043992">
        <property type="term" value="F:histone H3K9 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010515">
        <property type="term" value="P:negative regulation of induction of conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05509">
        <property type="entry name" value="Bromo_gcn5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000046">
        <property type="entry name" value="Histone acetyltransferase GCN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000004">
        <property type="entry name" value="Histone acetyltransferase KAT2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 14" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="49"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="16">
        <location>
            <begin position="294"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="18">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA21513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="389" mass="45294" checksum="E2289A0677910543" modified="2016-10-05" version="1" fragment="single">KNDDNEPDDEPEDDDEEEETRRVTVFNFDGMEYAFKERPSVLEEREGKIEFRVVNNDNTRENMIVLTGLKNIFQKQLPKMPREYILRLVYDRLHLLMAVVRKPLTVVGGITYRLFDSRGFAEIVFCAISLTEQVRGYGAHLMNHLKDYVRATSPVKYFLTYADNYAIGYFKKQGFTKEVTLDKLVWVGYIKDYEGGTLMQCLMLPSRLRYLDSGKILLLQKAAIEKQIKMRSTSHVVRPGLQVFKNARPGFKLLASEIPGLIASGWLEEMDKLAQKPKRGPHYNFMVSLLSELTNHPLAWPFAVPVNKDEVGDYYDVIKEPMDLSTMELKLENDKYDTFDQFLYDCRLIFNNCRSYNAETTTYYKNATKLDKFLTKKVKESADYSHFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC97</accession>
    <name>A0A1A0HC97_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DUF747-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_42826</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712264.1">
        <property type="nucleotide sequence ID" value="XM_018857591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC97"/>
    <dbReference type="GeneID" id="30030567"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="455"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="510"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="670" mass="75770" checksum="6E1A0423EF98A775" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCB4</accession>
    <name>A0A1A0HCB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Metallo-dependent phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_26299</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712146.1">
        <property type="nucleotide sequence ID" value="XM_018855315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCB4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCB4"/>
    <dbReference type="GeneID" id="30028291"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291905" evidence="5">
        <location>
            <begin position="19"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="6">
        <location>
            <begin position="48"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="347" mass="40417" checksum="F1C8B791D6506178" modified="2016-10-05" version="1" fragment="single">RVMLILATYWMLVVHVFERVRVRTAMDTCQWEKWENWTGDQALVAPHRVAFLADPQLVDDHTYPKMPRVLNYLVRKMSDNYHYLNYKYLQEILDPHTTVFIGDLFDGGRDWQDGPWLEEFHRFCRIFPEKRGRKSYRGLPGNHDIGFQNVSPDVAQRFASYFGEPNDVYILGNHTFIQLDTISLSHEDPRVHTAPRQFLEHAKTLLDPSFPRVVLTHVPLYRDPHVETCAPGRESKNPFPLMRGVEYQTAIEYPFSEDILRNLKPVLVFSGDDHDYCDMTHLDYADNTKRLAREISCKTNSMTNGIKRPAVQLLSLNNPQDGIGNTEKTYETTMCYLPSPYLGVKVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCE0</accession>
    <name>A0A1A0HCE0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="12">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_42262</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712062.1">
        <property type="nucleotide sequence ID" value="XM_018857520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCE0"/>
    <dbReference type="STRING" id="869754.A0A1A0HCE0"/>
    <dbReference type="GeneID" id="30030496"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030312">
        <property type="term" value="C:external encapsulating structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000020">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 9 12" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="13">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="10">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA21552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="333" mass="35615" checksum="865FAF971FFF0854" modified="2016-10-05" version="1">MAITIGINGFGRIGRLVLRVALSRKDIKVVAVNDPFIAPEYAAYMFKYDSTHGKYEGEVTSKDDKLVIDGQEITVFGERDPALIPWGKAGVDYVIESTGVFTTLEGAQKHIDGGAKKVIITAPSSDAPMFVVGVNHTKYAGENIVSNASCTTNCLAPLAKVIDDVFGIEEGLMTTVHSITATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKVIPKLNGKLTGMSLRVPTVDVSVVDLTVRLKKATSYEEISQAIKKASEGELKGVLGYTEDAVVSTDFLGSSYSSIFDQKAGILLSPTFVKLISWYDNEFGYSTRVVDLLEHVAKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI3</accession>
    <name>A0A1A0HCI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41953</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.67" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712210.1">
        <property type="nucleotide sequence ID" value="XM_018857469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI3"/>
    <dbReference type="STRING" id="869754.A0A1A0HCI3"/>
    <dbReference type="GeneID" id="30030445"/>
    <dbReference type="OrthoDB" id="16692at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016314">
        <property type="term" value="F:phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051896">
        <property type="term" value="P:regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14497">
        <property type="entry name" value="PTP_PTEN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051281">
        <property type="entry name" value="Dual-spec_lipid-protein_phosph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057023">
        <property type="entry name" value="PTP-SAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305">
        <property type="entry name" value="PHOSPHATASE WITH HOMOLOGY TO TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12305:SF81">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE 3-PHOSPHATASE AND DUAL-SPECIFICITY PROTEIN PHOSPHATASE PTEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22784">
        <property type="entry name" value="PTP-SAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatase tensin-type" evidence="4">
        <location>
            <begin position="16"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="107"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="368" mass="42512" checksum="E6471FF11D86A4FA" modified="2016-10-05" version="1">MNPENIIRALVSVPKKGYYDAEDFLDLSYVTPRIIVAAGPSDNFVSNIFRSPVDRVVSHLNRYRSGDQAHWHIWNLRGEGSGYSVNDIPAEYWSYYPFPDQQPPSVTFLISITREISDFFQLSPRNVALIHCREGKGRSGTVCCALLMYEAKLKGIYMSVDEAIAMFTRRRMRKYFGPGVLIYSQVRYLSYWKTYLLLSDNMIKNYQEFDSHGSVPFDISRSVITKITVIRPTLLLVLSKFKILTYTEKGNGLEEELLRTQSLGLPHISANTSFLDVPMNIPITAHMKDIKISFERQFCIAYAWFNLFFETLGDSKRPLPHQGASYITAKRLVLPWELFDGTRGFKVNSNFKLFDRIEILWVYHFPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCI4</accession>
    <name>A0A1A0HCI4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_188459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712107.1">
        <property type="nucleotide sequence ID" value="XM_018854818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCI4"/>
    <dbReference type="GeneID" id="30027794"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="100" mass="10661" checksum="2D4DED0BB49D0995" modified="2016-10-05" version="1">MPYEAGTKNGWAAKTAMIGEGAFFFLPPHTPQGPARARPPAGAWASNREPGQQPRPSTQARYPGPVRIVLYLRGLAFAPVSLASLSRRATVQPAPLEPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCL9</accession>
    <name>A0A1A0HCL9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Eukaryotic translation initiation factor 3 subunit H</fullName>
            <shortName evidence="1">eIF3h</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_32203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-3 subunit H family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712250.1">
        <property type="nucleotide sequence ID" value="XM_018856005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCL9"/>
    <dbReference type="STRING" id="869754.A0A1A0HCL9"/>
    <dbReference type="GeneID" id="30028981"/>
    <dbReference type="OrthoDB" id="10265695at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08065">
        <property type="entry name" value="MPN_eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03007">
        <property type="entry name" value="eIF3h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027524">
        <property type="entry name" value="eIF3h"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045810">
        <property type="entry name" value="eIF3h_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19445">
        <property type="entry name" value="eIF3h_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="2">
        <location>
            <begin position="18"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="333" mass="37920" checksum="3DBB16C1C20A3041" modified="2016-10-05" version="1">MASKQADRAFEAPTATSIDIEGSVLLSLIRHTSENYPQLYSGSLLGFEDDSGAVDVTHAYPFPYPDQYEGGSLRSRGGAKYQQEILDALVSLGHGVEFQGWFQSTISGNFITSQLVEALAQQQQVNKNAFILIHNMASIGKEVEVKALRLSENFLATYLKGKWKTRDLENNKLSFFNIFDEITINIHNQHLVNMYLAQSDETSTLGESENLTFNSNSNMTTQLLENLCSQIDSYNFDQTNFNYYQRQLQKEHTKILQWKQNRKAENDERAKIGEPELDPEEWQTIFKGPNGPSRLNNTLYSRSIDEFADDILKQCDEELLKSFAIERKLTAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCN0</accession>
    <name>A0A1A0HCN0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_192260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712266.1">
        <property type="nucleotide sequence ID" value="XM_018854848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCN0"/>
    <dbReference type="GeneID" id="30027824"/>
    <dbReference type="OrthoDB" id="4092221at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="494" mass="55516" checksum="4F4F9C39E5D1FD7D" modified="2016-10-05" version="1">MLLDGTALIANAIYIYLIAVFSSVVLAVTALIIRGFVRRGRGHGDISGKKLELAVASKLKFRANAEFPCANSTLKESKNLLRRWKSRNGHTTIAQVKPQSIEINEVESSATANDCAPISNDQSLEASMPVRPRIIYPLEDLKSMNVNIAQLTFEPNKIRLSNLLIPNITLPRSRIIDKYAMDQELKLVQILLNDPLLSNRCMGLFRVVSATSFSEPYTNAQHFHGGLCDLLETIIDTLVIYSTSDTIPSLQLALLDLAFVFLWEHWNWDQGFRLAVAAKLAQETIVSAGATAGTVTIQEVVPNDSMHFKTEKMSQYAEYEFAKHVEPVVLLEFSLFRLFCNLYLGTPVMAKPIRNRCMFKLSGIFYCCASQTKCHLHVELAKLLGQALHSTERDSIKAFFYEVLLKVLQPHVYCCIHPDDIFSNGSIIQKLIFQGLWTDQPPSVRDKAADVVEMLSERFHEIVSWNKSMKFTHLNDEHGARQHGGAPGSWLTFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCW1</accession>
    <name>A0A1A0HCW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">chitin deacetylase</fullName>
            <ecNumber evidence="6">3.5.1.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_77384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + n H2O = chitosan + n acetate</text>
            <dbReference type="Rhea" id="RHEA:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9597"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57704"/>
            <dbReference type="EC" id="3.5.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:10465"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.41" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712316.1">
        <property type="nucleotide sequence ID" value="XM_018858687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCW1"/>
    <dbReference type="STRING" id="869754.A0A1A0HCW1"/>
    <dbReference type="GeneID" id="30031663"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005628">
        <property type="term" value="C:prospore membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030476">
        <property type="term" value="P:ascospore wall assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050248">
        <property type="entry name" value="Polysacc_deacetylase_ArnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF133">
        <property type="entry name" value="CHITIN DEACETYLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0146">Chitin degradation</keyword>
    <keyword evidence="5" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291908" description="chitin deacetylase" evidence="8">
        <location>
            <begin position="17"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="9">
        <location>
            <begin position="93"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="296" mass="33593" checksum="DAE47497AED4807A" modified="2016-10-05" version="1">MLCKPFCFFFFSSVVGIPLPFPEWLSELTGLSNWPGVDPPYIPLDFVDFSVIPGELESWVHTQGSCQSIENTADVCSFDCWACATEEDVVTCPVLSQTFDDGPSPFTLDLIDKLETPVTFFTIGINVVRFPHVYRKTANKGHIMASHTWSHKFLPSLTNEEIIAQIEWSVWAMNATQHHLPKWFRPPYGGIDNRIRSILHHFGMQAVLWDFDSLDWALAAGQGKETEEELHERLLDFSEKRNNKGLILEHDSFEKTVNIGKGLYELVGSDQLTVPQCVEGIDYIKTWDSDCESCLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD46</accession>
    <name>A0A1A0HD46_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_39660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712500.1">
        <property type="nucleotide sequence ID" value="XM_018857165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD46"/>
    <dbReference type="STRING" id="869754.A0A1A0HD46"/>
    <dbReference type="GeneID" id="30030141"/>
    <dbReference type="OrthoDB" id="3996251at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34141:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="63" mass="6966" checksum="57FA2DC2CF2DC905" modified="2016-10-05" version="1" fragment="single">PTLRANPYPEVTDLFCRLPLSTLFYQLEAVNLGDLLRISVRLGMKVNPSCGFSRTVVGAPDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD48</accession>
    <name>A0A1A0HD48_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavo protein WrbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_39407</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flavodoxin-like protein (FLP) that plays a role in cell wall integrity, oxidative stress protection and virulence. FLPs act as NAD(P)H quinone oxidoreductases. Reduces ubiquinone (coenzyme Q), enabling it to serve as an antioxidant in the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712318.1">
        <property type="nucleotide sequence ID" value="XM_018857135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD48"/>
    <dbReference type="STRING" id="869754.A0A1A0HD48"/>
    <dbReference type="GeneID" id="30030111"/>
    <dbReference type="OrthoDB" id="504689at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000001">
        <property type="entry name" value="NAD(P)H dehydrogenase (Quinone) FQR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002999">
        <property type="entry name" value="PRK03767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546">
        <property type="entry name" value="FLAVODOXIN-RELATED PROTEIN WRBA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30546:SF23">
        <property type="entry name" value="FLAVOPROTEIN-LIKE PROTEIN YCP4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="4">
        <location>
            <begin position="5"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00053955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="200" mass="21401" checksum="D154D83E06457449" modified="2016-10-05" version="1">MAPKVAIITYSLYRHVATMAESVKKGVEIAGGKADIYQIAETLSDEILTKMHAPSKPDYPIATLDTLTLYDAFLFGVPTRFGNYPAQWKTFWDSTGGLWASGALHGKPAGFFVSTGTPGGGQETTIINALSVVVHHGMIYVPLGYANAFGQLTNMEEVHGGSPFGAGTYAGVDSSRTPSTLELEIAKIQGSSFFKTIQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDA0</accession>
    <name>A0A1A0HDA0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pkinase-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_26714</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712404.1">
        <property type="nucleotide sequence ID" value="XM_018855326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDA0"/>
    <dbReference type="STRING" id="869754.A0A1A0HDA0"/>
    <dbReference type="GeneID" id="30028302"/>
    <dbReference type="OrthoDB" id="68483at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14008">
        <property type="entry name" value="STKc_LKB1_CaMKK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895">
        <property type="entry name" value="CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43895:SF152">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE TOS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="23"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="485"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="615"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="774"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="970"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="774"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="972"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1012"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1012" mass="111304" checksum="70BF2D71E3E1DCDF" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDQ1</accession>
    <name>A0A1A0HDQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77591</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712601.1">
        <property type="nucleotide sequence ID" value="XM_018858727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDQ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HDQ1"/>
    <dbReference type="GeneID" id="30031703"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051268">
        <property type="term" value="F:alpha-keto amide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051269">
        <property type="term" value="F:alpha-ketoester reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016652">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19120">
        <property type="entry name" value="AKR_AKR3C2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044494">
        <property type="entry name" value="AKR3C2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="304" mass="33863" checksum="A9EEC8F8D4F61E3B" modified="2016-10-05" version="1">MSLQGSRFQLADGHKIPAVAYGLGTAWFKYGRNDEDQRLVQTLQLALEKGFVHIDGAEIYNTDAELGAAIAGADRAKLYITNKFLVGDAGHSVFSPHGLPYDSLKHQLATQMKTGYVDLYLLHSPFVAKAAHGFDLAEAWRSMEKIVDEGLARSIGVSNFRVEDLQEILKIARIRPVVNQIEYNALLQNQTPGIVEFSQQNGILVEAYSPLGPITKGPAGELSDYLQQLADTHHKTPAQVLLRWVLQRGILPVTTSSKEDRIVQFLDLFDFELSESEAQHITVLGKKGPVLRQYWLAEFGKYDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDU3</accession>
    <name>A0A1A0HDU3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">IMS import disulfide relay-system CHCH-CHCH-like Cx9C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712646.1">
        <property type="nucleotide sequence ID" value="XM_018857094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDU3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDU3"/>
    <dbReference type="GeneID" id="30030070"/>
    <dbReference type="OrthoDB" id="276296at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2900">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052848">
        <property type="entry name" value="CHCH_domain-containing_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031731">
        <property type="entry name" value="CX9C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47106:SF1">
        <property type="entry name" value="COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16860">
        <property type="entry name" value="CX9C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IMS import disulfide relay-system CHCH-CHCH-like Cx9C" evidence="1">
        <location>
            <begin position="11"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="101" mass="11089" checksum="0727505F244D81C9" modified="2016-10-05" version="1">MSSSALDQIMIEDIARYCPQQFLAFHQCMSKPPSESDCAVEQVNLSRCIKGSVPVFQKIHGVCAGKLQAYELCLRMNGSAQKMCQQDLSELRDCALGAVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDX7</accession>
    <name>A0A1A0HDX7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712712.1">
        <property type="nucleotide sequence ID" value="XM_018857170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDX7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDX7"/>
    <dbReference type="GeneID" id="30030146"/>
    <dbReference type="OrthoDB" id="5367584at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024312">
        <property type="entry name" value="TACC_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12709">
        <property type="entry name" value="Fungal_TACC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="261"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="314" mass="36624" checksum="0B338284E641E56D" modified="2016-10-05" version="1">MYVLKFEQETANLKLLTQRSNDELHTFKVKFEHASQEIDVLKNLKSSLISQNEAKDDEICALKTEIEEKDSLNRTLDQKFSKLMEDTRHLHHEMELIEREHNALKYEITEKNQAIDALELLRGELEHKCLCHQEEAESNKSSALKFENESKRLDSILMEANAQLHDLKSQSKEKDSIISGDTQKLAELNAQVNAQKKMISDLKAEVLRTNTGHEHSEAELIHEVETLKAQIFAAQQKSDERIQEIAEQLYHQYSKKHEVKVGQLRKNYEFKIKEMKEVSEESEKRIENLESQLQIEVKEKDYLLSVLEKVKENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDY8</accession>
    <name>A0A1A0HDY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_31142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712691.1">
        <property type="nucleotide sequence ID" value="XM_018855778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDY8"/>
    <dbReference type="STRING" id="869754.A0A1A0HDY8"/>
    <dbReference type="GeneID" id="30028754"/>
    <dbReference type="OrthoDB" id="2133190at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052099">
        <property type="entry name" value="Regulatory_TF_Diverse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47336:SF2">
        <property type="entry name" value="TRANSCRIPTION FACTOR HMS1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BHLH" evidence="2">
        <location>
            <begin position="177"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="302" mass="33759" checksum="30FF5E526DC0A20F" modified="2016-10-05" version="1">MVQSNQSVNHFDSSEIARGQLKNSTKSFHPRVSSYITFQTNSNTPPNLNPENSELEMTKYQNLPLGLFSKVNAGLPPKLNNFQEEVLDLSSDFNSQQNIRDGLLSSLALYTGSANKESTSFSTSSTSTQQNKIKGSFEKRRIEVANTEHTNMPDCEDPRTTIDSLGHASEAPANKVKLKSSHNVIEQRYRNKINDKFTALQHSVPTLRAAVEKKDKLKVDEGIKGSELEEVSDLSDSSTSLEGLQPATKLNKGTILAKTVEYIKFLEGKNKSLTMEYEQILTRARMLGLRYDENSDYIEKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE49</accession>
    <name>A0A1A0HE49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_11226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712880.1">
        <property type="nucleotide sequence ID" value="XM_018853936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE49"/>
    <dbReference type="GeneID" id="30026912"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="129" mass="13266" checksum="5185B9105449CD2B" modified="2016-10-05" version="1">MGVGIVGIYWCGYLLVFIGVLLLVVIGVEVGVGVIGVIGVDTGVVFIEIDIYWFDTGVGIDSGIGVYLLVHIGTGTAVVMVSVGMIGVGIIGVGIIGVGIIGVGIIGVGIIGVGDYWCWYLLAGVYWYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HES7</accession>
    <name>A0A1A0HES7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_31500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713125.1">
        <property type="nucleotide sequence ID" value="XM_018855866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HES7"/>
    <dbReference type="GeneID" id="30028842"/>
    <dbReference type="OrthoDB" id="4096350at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="258" mass="28259" checksum="8DCBA7A168CEB150" modified="2016-10-05" version="1">MAVLVDVLRLVVHKSERKGALCRRAEYVAKYKDTVRSLGGGTPGQRADSGVALPDVHADYPPAFEVAVVQNGDDIDEADYLAAKHVATQQMLRLVALKHGVRFAALSGLLLLAENTTVVAAAAHWLFRTALGTDRVVYTPEGSVLVGPVLVHQYIPVGWDSWAKILLVARSAPRLAVMARLFGTEAEIRRFNEAYDGCFEDVHAMGFLESEDGYVLQAAQARLMPYLDEGPGPRLPEKPALPMTYGQFLQRLHARQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEY7</accession>
    <name>A0A1A0HEY7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_153919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712969.1">
        <property type="nucleotide sequence ID" value="XM_018854594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEY7"/>
    <dbReference type="GeneID" id="30027570"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="10351" checksum="0AA9BE6D9F0A3043" modified="2016-10-05" version="1">MLLASLQSRCFLCIRKHIYRLLVLESELKERNFMLVYYFVFFLFFTHLIVSSEIHENLKHLTCSNFKCYSCNKKNKSKQTLFGTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF22</accession>
    <name>A0A1A0HF22_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mitochondrial protein PET191</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_75918</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PET191 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713210.1">
        <property type="nucleotide sequence ID" value="XM_018858446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF22"/>
    <dbReference type="STRING" id="869754.A0A1A0HF22"/>
    <dbReference type="GeneID" id="30031422"/>
    <dbReference type="OrthoDB" id="282149at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018793">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_Pet191"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28627:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10203">
        <property type="entry name" value="Pet191_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13529" checksum="425D4CD42B0269C9" modified="2016-10-05" version="1">MGASCQDQKKALAICLQRSPCVLIERNTPKKCLTDPKLKKELPELCVANFKAFMKCKNGFFDMRKRMRGNAPLSTGKYDEMYEKLSTGDFDAHEEMRKLDMLNKNLAKEKAYPEKSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF30</accession>
    <name>A0A1A0HF30_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Altered inheritance of mitochondria protein 36, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_77881</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712998.1">
        <property type="nucleotide sequence ID" value="XM_018858784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF30"/>
    <dbReference type="GeneID" id="30031760"/>
    <dbReference type="OrthoDB" id="4081130at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="28215" checksum="7F3BF9D98FA62A27" modified="2016-10-05" version="1">MFKARSRPGFHYTNFTKQAVQGSTSKKRQKVPRVRYLFAVVLLSYGCLYFVTSKMTKKAPRTSFTEREFEQYEKDSGLKRRLKLVPVEKNNQYTFYAVPFAHDQSKILELLQTVVPEGKQMKVIHPQLLIQKEMEEQGKYSYLLEDLKAQGRSLPRGLVTALVKQEVQLFMNTTGGQYDTTIVLMNYPQTTEEAIKFENDVADVHSCVVASGDFDENLAADMSVDSVRQINNVVGYFDIVDKVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF92</accession>
    <name>A0A1A0HF92_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_40576</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in DNA replication and cell separation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SDS23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713049.1">
        <property type="nucleotide sequence ID" value="XM_018857288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF92"/>
    <dbReference type="STRING" id="869754.A0A1A0HF92"/>
    <dbReference type="GeneID" id="30030264"/>
    <dbReference type="OrthoDB" id="449052at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030071">
        <property type="term" value="P:regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050511">
        <property type="entry name" value="AMPK_gamma/SDS23_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016711">
        <property type="entry name" value="Ssd23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780">
        <property type="entry name" value="AMP-ACTIVATED PROTEIN KINASE, GAMMA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13780:SF36">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018148">
        <property type="entry name" value="UCP018148_CBS_YBR214w"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="200"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="275"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="392"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR018148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="518" mass="56733" checksum="AFCB36F0AD68E7F1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFB8</accession>
    <name>A0A1A0HFB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_19161</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713156.1">
        <property type="nucleotide sequence ID" value="XM_018854847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFB8"/>
    <dbReference type="GeneID" id="30027823"/>
    <dbReference type="OrthoDB" id="3026777at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507:SF1">
        <property type="entry name" value="FI18259P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23507">
        <property type="entry name" value="ZGC:174356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="457"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="493"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="538" mass="58135" checksum="5CA5368714FAFA70" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFH4</accession>
    <name>A0A1A0HFH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="3 12">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_38220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713380.1">
        <property type="nucleotide sequence ID" value="XM_018856971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFH4"/>
    <dbReference type="STRING" id="869754.A0A1A0HFH4"/>
    <dbReference type="GeneID" id="30029947"/>
    <dbReference type="OrthoDB" id="1649088at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120513">
        <property type="term" value="C:2-(3-amino-3-carboxypropyl)histidine synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11850:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="407" mass="46168" checksum="BEC5FF63F4851588" modified="2016-10-05" version="1">MNFTGDKPKKRFVGRRVAESSNASLVQAPKRHIGRALNQIPDDILGDKDLNEAIRLLPSNYNFEIHKCVWNIKKHNSRRVALQMPEGLLIYSMIISDILEQFCGVETVVMGDVSYGACCIDDFTARALDCDFIVHYAHLCLVPIDVTKIKVLYVFVTINIDETHLMNTLKKNFHQGDHLACFGTIQFNPTIHSISAKLGNDIEKPIYIIPPQTMPLSKGEVLGCTSARLDENIKAMVYVGDGRFHLESAMIHNPNIPAYRYDPYSRKFTREHYDQQQMVQVRQDAMNAAAKAKKVGLILGALGRQGNPQTLDRIETTFKEKGVEVVKIILSEIFPQKLAMFSDIDAFVQVACPRLSIDWGYAFEKPLLTPYEAMVVTGNDTWLKEYYPMDYYEKDGYGRGKVPLRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFK6</accession>
    <name>A0A1A0HFK6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sure-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713415.1">
        <property type="nucleotide sequence ID" value="XM_018856729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFK6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFK6"/>
    <dbReference type="GeneID" id="30029705"/>
    <dbReference type="OrthoDB" id="202825at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027746">
        <property type="entry name" value="TTL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47551:SF1">
        <property type="entry name" value="TUBULIN--TYROSINE LIGASE PBY1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="636"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="704" mass="78990" checksum="7C95A2723EF81A49" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFR7</accession>
    <name>A0A1A0HFR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LSM1</name>
        <name evidence="9" type="ORF">METBIDRAFT_34585</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713311.1">
        <property type="nucleotide sequence ID" value="XM_018856492.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFR7"/>
    <dbReference type="STRING" id="869754.A0A1A0HFR7"/>
    <dbReference type="GeneID" id="30029468"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000045">
        <property type="entry name" value="U6 snRNA-associated Sm-like protein LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="8">
        <location>
            <begin position="49"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="185" mass="21140" checksum="97F3F99BF85EE01F" modified="2016-10-05" version="1">MSSSPQQVSPSLQHPNPQHSSAPHSQHSLSELGGSSNAEDLYLESYAFTTAAAIVGSVDRKIFVLLRDGRSVFGILRTFDQFANLVLQDTLERIYLPPKQAPGPERFAEAPRGVFMVRGENVVMLGELDIDREDFHLYEREQISFEEAERELKARQLQRVRDEKTRARNYLKRGLIHDFHKADLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG34</accession>
    <name>A0A1A0HG34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Plasma membrane fusion protein PRM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_34513</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Involved in cell fusion during mating by stabilizing the plasma membrane fusion event.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the PRM1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713442.1">
        <property type="nucleotide sequence ID" value="XM_018856480.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG34"/>
    <dbReference type="STRING" id="869754.A0A1A0HG34"/>
    <dbReference type="GeneID" id="30029456"/>
    <dbReference type="OrthoDB" id="5356111at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032220">
        <property type="term" value="P:plasma membrane fusion involved in cytogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026777">
        <property type="entry name" value="PRM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31030:SF1">
        <property type="entry name" value="PLASMA MEMBRANE FUSION PROTEIN PRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0184">Conjugation</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="555"/>
            <end position="577"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="653" mass="72156" checksum="23BC1E461E20C992" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG35</accession>
    <name>A0A1A0HG35_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosome biogenesis protein NSA2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the pre-66S ribosomal particle. Interacts with NOP7 and RRP1. Interacts with RSA4 (via WD repeats).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eS8 family. Ribosome biogenesis protein NSA2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713338.1">
        <property type="nucleotide sequence ID" value="XM_018858462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG35"/>
    <dbReference type="STRING" id="869754.A0A1A0HG35"/>
    <dbReference type="GeneID" id="30031438"/>
    <dbReference type="OrthoDB" id="1847590at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11381">
        <property type="entry name" value="NSA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000001">
        <property type="entry name" value="NSA2, ribosome biogenesis homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039411">
        <property type="entry name" value="NSA2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12642">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NSA2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="261" mass="29660" checksum="B446AC2FB47224C9" modified="2016-10-05" version="1">MPQNEYIEQHIKQHGRRLDHEERKRKKEAREGHRVAKDAQNLKGWRGKQFAKKRYSEKVAMKKKIKAHQESKVKGPSTPKQDDGEALPTYLLDRETNNTAKAISSSIKQKRMEKADKFSVPLPKVRGISEEEMFKVIKTGKSKTKSWKRMITKHTFVGEGFTRRPVKMERIIRPSALRQKKANVTHPELAVTVFLPILGVKKNPQSPMYTQLGVLTKGTIIEVNVSELGLVTAGGKVVWGKYAQITNEPDRDGCVNAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGB4</accession>
    <name>A0A1A0HGB4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Conserved oligomeric Golgi complex subunit 6</fullName>
            <shortName evidence="10">COG complex subunit 6</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">Component of oligomeric Golgi complex 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_76049</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Acts as component of the peripheral membrane COG complex that is involved in intra-Golgi protein trafficking. COG is located at the cis-Golgi, and regulates tethering of retrograde intra-Golgi vesicles and possibly a number of other membrane trafficking events.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the conserved oligomeric Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the COG6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713379.1">
        <property type="nucleotide sequence ID" value="XM_018858468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGB4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGB4"/>
    <dbReference type="GeneID" id="30031444"/>
    <dbReference type="OrthoDB" id="272987at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010490">
        <property type="entry name" value="COG6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048369">
        <property type="entry name" value="COG6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048368">
        <property type="entry name" value="COG6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506">
        <property type="entry name" value="COMPONENT OF OLIGOMERIC GOLGI COMPLEX 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21506:SF0">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20653">
        <property type="entry name" value="COG6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06419">
        <property type="entry name" value="COG6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01087">
        <property type="entry name" value="COG6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric complex COG6 N-terminal" evidence="11">
        <location>
            <begin position="145"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved Oligomeric Golgi complex subunit 6 C-terminal" evidence="12">
        <location>
            <begin position="277"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="752" mass="85357" checksum="8E06DE1BCBD5645B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGF5</accession>
    <name>A0A1A0HGF5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_35539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713443.1">
        <property type="nucleotide sequence ID" value="XM_018856622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGF5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGF5"/>
    <dbReference type="GeneID" id="30029598"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16116">
        <property type="entry name" value="Ubl_Smt3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000155">
        <property type="entry name" value="Ubiquitin-like protein SMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="12"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="90" mass="10152" checksum="79F6E9A0E66AF504" modified="2016-10-05" version="1">MAQEAKEEVSDTHINLKVSDGAGEIFFKIKRTTPMKRLMDAFCKRQGRSLDSIRFLVDGTRIHPDATPQEMDLEDGDVIEAHREQTGGCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGN3</accession>
    <name>A0A1A0HGN3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNase I-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713642.1">
        <property type="nucleotide sequence ID" value="XM_018855460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGN3"/>
    <dbReference type="GeneID" id="30028436"/>
    <dbReference type="OrthoDB" id="276515at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09083">
        <property type="entry name" value="EEP-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF36">
        <property type="entry name" value="ENDONUCLEASE_EXONUCLEASE_PHOSPHATASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="55"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="276" mass="31434" checksum="43D9FAD032FCF32B" modified="2016-10-05" version="1">MLLTYQWHRSPAGRPLEIRLYTNNIRFDNKNLSPGERLWESRGPLAAQSIEENAGHGPSVVCLQEVLHNQLKDILSHLNKDGQEWGYYGVGRSDGRTKGEYAPILYKKADWHLESSRTYWLSETPDKPSVGWDAALERIVTEAILKSKISGQSVKVLNTHFDHKGVVARRELVKLIISKLEAGDEPSFLCGDLNTQPTDEPYAILKKSDFRDSRTFMLNPDENEPTFTGFSDAEGTGIIDYVWADSKSHWRGYHVLPNFFGFRMSDHRPVAADFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGR6</accession>
    <name>A0A1A0HGR6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_28796</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713529.1">
        <property type="nucleotide sequence ID" value="XM_018855362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGR6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGR6"/>
    <dbReference type="GeneID" id="30028338"/>
    <dbReference type="OrthoDB" id="4142200at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF7">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="183"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="380" mass="41357" checksum="97D63A61AC2F24C8" modified="2016-10-05" version="1" fragment="single">VSALFTGQLCDRFGHLHLMRLAGFLWCLGCLGTYFSPENVYIVLCKLGKGFGLGMASVAVPLYVFDVLVAPYRQLAIGWAAAFSIFGQAAMYAFGILLRTTLGSAVDAFHYSWIAEVFWGVLLVGPSLFLPDSPKFLARKGQWERATASLERLSLQKNLSLTSVPLQPSTFGDLFRRENRKRFFFAVALQAMAHGACVFGATHFIHYTLAFCRLDTALVETLYGVFFAIQLLFTALSVFVMKNSRRKDMLAYGFFLLTVAFSSLGATSYVYATGSTNYFVYAPVVQVSLVAASTVLALVGFAFSVASLLITSLSLLCTLETLPCDLRAKGVSIAMSTAWTVETLAATLSIGLTHIAPFALFFGLAVVSVAAIVVTLYVYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGT7</accession>
    <name>A0A1A0HGT7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="10">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_36527</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the phage and mitochondrial RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713870.1">
        <property type="nucleotide sequence ID" value="XM_018856751.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HGT7"/>
    <dbReference type="GeneID" id="30029727"/>
    <dbReference type="OrthoDB" id="276422at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034245">
        <property type="term" value="C:mitochondrial DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001018">
        <property type="term" value="F:mitochondrial promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1320.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000041">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.280:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1320.10">
        <property type="entry name" value="DNA-directed RNA polymerase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024075">
        <property type="entry name" value="DNA-dir_RNA_pol_helix_hairp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046950">
        <property type="entry name" value="DNA-dir_Rpol_C_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002092">
        <property type="entry name" value="DNA-dir_Rpol_phage-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037159">
        <property type="entry name" value="RNA_POL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029262">
        <property type="entry name" value="RPOL_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10102:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00940">
        <property type="entry name" value="RNA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14700">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01311">
        <property type="entry name" value="RPOL_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00900">
        <property type="entry name" value="RNA_POL_PHAGE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00489">
        <property type="entry name" value="RNA_POL_PHAGE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase N-terminal" evidence="11">
        <location>
            <begin position="374"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1308" mass="148558" checksum="CFF316D091A6CF0F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH21</accession>
    <name>A0A1A0HH21_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_29805</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713777.1">
        <property type="nucleotide sequence ID" value="XM_018855483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH21"/>
    <dbReference type="STRING" id="869754.A0A1A0HH21"/>
    <dbReference type="GeneID" id="30028459"/>
    <dbReference type="OrthoDB" id="188276at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070899">
        <property type="term" value="P:mitochondrial tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14858">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000007">
        <property type="entry name" value="tRNA modification GTPase GTPBP3, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="entry name" value="tRNA modification GTPase MnmE domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF2">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="GTP-binding protein TrmE N-terminal" evidence="7">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="MnmE helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="G" evidence="6">
        <location>
            <begin position="227"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="423" mass="45730" checksum="46B051E87ED6BD10" modified="2016-10-05" version="1" fragment="single">MARPTIYALSTKPGRAALGVIRVSGPGASHVFRQLTKSPRQPQHRMASVRSLYGRKGLLDHALTLFFQGPRSYTGEDVLELHVHGGTAIVKAVMDAVGHSHRPAEGIHVRYAEQGEFSRRAFLNGRFDLTEIEGIREMIDAETESQRVGALASLTGSTLALMQRWRHDIVRNVALLTTVIDFGEEHDLEDTQRLFGDVGRSIDALAADIRGYLRKVRGSEVLMRGIKVTLVGPPNAGKLSLLNCLAHSDVAIVSDIAGTTRDVIDVPLDIEGYKIVVGDTAGIRALLDAGLIEKEGIRRAKQKALVGDMVLVVAPVDEPLARDILDHVALLHEAKRHVVAVVNKVDLASGPALAAAREKLAAQLRIPPASVFAVSCLTGEGMAPLRAELVLLFKRVSMTTSSDPVVISARAQDLLEHDVLYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH34</accession>
    <name>A0A1A0HH34_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_9748</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713970.1">
        <property type="nucleotide sequence ID" value="XM_018859454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH34"/>
    <dbReference type="GeneID" id="30032429"/>
    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02340">
        <property type="entry name" value="ZZ_NBR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052260">
        <property type="entry name" value="Autophagy_Rcpt_SigReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15090">
        <property type="entry name" value="SEQUESTOSOME 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00569">
        <property type="entry name" value="ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00291">
        <property type="entry name" value="ZnF_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="192"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="551"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="603" mass="67295" checksum="FBDCAC06F06C597F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH66</accession>
    <name>A0A1A0HH66_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_102149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713703.1">
        <property type="nucleotide sequence ID" value="XM_018853713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH66"/>
    <dbReference type="GeneID" id="30026689"/>
    <dbReference type="OrthoDB" id="4075484at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="571" mass="62633" checksum="57C0E3439F752CB0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH84</accession>
    <name>A0A1A0HH84_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_20649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713842.1">
        <property type="nucleotide sequence ID" value="XM_018854994.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH84"/>
    <dbReference type="STRING" id="869754.A0A1A0HH84"/>
    <dbReference type="GeneID" id="30027970"/>
    <dbReference type="OrthoDB" id="3363151at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="185" mass="20772" checksum="F5CF0AB1E0F1C026" modified="2016-10-05" version="1" fragment="single">APASKTVHDDPVGHLQNDLAFVTHDINGIRHKSVRADNLDAVISRSVSPAKHLTQNSGILPPGSSFGSRYDTSLRSINGRSSFYDDTENRHSMVLLSTPFRLRNQGNELFLHRSDTFSRANDTFSKGYDSISRNSSRAHSPFKSPSRLNYNPNISYSERTQLSPDRSSRHLSPQRSPSPSKRVWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI45</accession>
    <name>A0A1A0HI45_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AP complex subunit sigma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_38534</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714156.1">
        <property type="nucleotide sequence ID" value="XM_018857015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI45"/>
    <dbReference type="STRING" id="869754.A0A1A0HI45"/>
    <dbReference type="GeneID" id="30029991"/>
    <dbReference type="OrthoDB" id="10261046at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030123">
        <property type="term" value="C:AP-3 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015588">
        <property type="entry name" value="AP_complex_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="7">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="154" mass="17517" checksum="D2725CE69F77EED4" modified="2016-10-05" version="1" fragment="single">KFYKPVDLLTQKTLLDQIYQLISVRTSKDCSFITPPKLLEDLDDIKVIYRHYATLYFVFVVDDQESELGILDLIQVFVECLDKCFTNVCELDLVFGWQVLQTVLEEIVQGGMVIDTNIARIVTAVDDANSQKLSGASVGTGWLSSLDSGFRWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIB2</accession>
    <name>A0A1A0HIB2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_36097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714059.1">
        <property type="nucleotide sequence ID" value="XM_018856688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIB2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIB2"/>
    <dbReference type="GeneID" id="30029664"/>
    <dbReference type="OrthoDB" id="162638at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_bL35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_bL35-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_bL35_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="L35p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="90" mass="10048" checksum="777A423CBD5AA9A5" modified="2016-10-05" version="1">MNQFLSTANKVIFGQRPAVSLLIQTRNKTKTCKAMAKRFIKTGSGYKRKQAGRNHGNGNFSAHSIRHLDSYVAVTNKGLHLKKLKKAMGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIK8</accession>
    <name>A0A1A0HIK8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">PAN2-PAN3 deadenylation complex catalytic subunit PAN2</fullName>
            <ecNumber evidence="10">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Poly(A)-nuclease deadenylation complex subunit 2</fullName>
            <shortName evidence="10">PAN deadenylation complex subunit 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PAN2</name>
        <name evidence="13" type="ORF">METBIDRAFT_9960</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 metal cations per subunit in the catalytic exonuclease domain.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Positively regulated by the regulatory subunit PAN3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase C19 family. PAN2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="10"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714203.1">
        <property type="nucleotide sequence ID" value="XM_018859499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIK8"/>
    <dbReference type="STRING" id="869754.A0A1A0HIK8"/>
    <dbReference type="GeneID" id="30032474"/>
    <dbReference type="OrthoDB" id="16516at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06143">
        <property type="entry name" value="PAN2_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000028">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex catalytic subunit PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03182">
        <property type="entry name" value="PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030843">
        <property type="entry name" value="PAN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050785">
        <property type="entry name" value="PAN2-PAN3_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048841">
        <property type="entry name" value="PAN2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028881">
        <property type="entry name" value="PAN2_UCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728">
        <property type="entry name" value="DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15728:SF0">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX CATALYTIC SUBUNIT PAN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20770">
        <property type="entry name" value="PAN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13423">
        <property type="entry name" value="UCH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="USP" evidence="12">
        <location>
            <begin position="581"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="999"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="1001"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="1109"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="1162"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA23722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1202" mass="134435" checksum="8A1B46829CFD3AF6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIW4</accession>
    <name>A0A1A0HIW4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_30305</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714427.1">
        <property type="nucleotide sequence ID" value="XM_018855608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIW4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIW4"/>
    <dbReference type="GeneID" id="30028584"/>
    <dbReference type="OrthoDB" id="648285at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000134">
        <property type="entry name" value="MFS sugar transporter protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF73">
        <property type="entry name" value="METABOLITE TRANSPORT PROTEIN YDL199C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="19"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="596" mass="66431" checksum="A67646E41A914135" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJD0</accession>
    <name>A0A1A0HJD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine proteinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_34203</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714473.1">
        <property type="nucleotide sequence ID" value="XM_018856450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJD0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJD0"/>
    <dbReference type="GeneID" id="30029426"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22762">
        <property type="entry name" value="OTU_fungi_OTU2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049771">
        <property type="entry name" value="OTU2-like_OTU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="153"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="299" mass="33311" checksum="9DDB53EA01C9167B" modified="2016-10-05" version="1">MEELLARHRKEQKDLIAQITSLKKQALKKTRKSVNTKCADLQSSLQARHAKEIADLSSDSSISTDSFTPEQLLAAMNLGSSATLEQAIEKTAHQESSSQSPGKKRNRARERLAKRQAQIDAMTAEAQAEAADSVDYRAIEAESMGLLLRALGLQMYEIQPDGHCLFRSIQDQLHHRHEFSADAADLSVSAIRRRAADHIRAHPDSYIPYLFDQETMSVQDVGEYTKELEETAMWGSDLEIAALAAVYECPIKVLVAGSAPIVFNEAGPKPELIVAYYKHSYGLGEHYNSCRDIERDVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF7</accession>
    <name>A0A1A0HJF7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_65453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714454.1">
        <property type="nucleotide sequence ID" value="XM_018858352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF7"/>
    <dbReference type="GeneID" id="30031328"/>
    <dbReference type="OrthoDB" id="4084571at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="98" mass="11184" checksum="D6C71BBA1DD0DE06" modified="2016-10-05" version="1">MPLPQGSPFSHKIIVSTIATAVGIYGFWLTAKDFEFVKFVPRSPEEIEIRRKENIGLQIKHLETRTLDYTPEAKARIAQSLKEYQTMTETKEPAESSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJI2</accession>
    <name>A0A1A0HJI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RhoGEF-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_115945</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714479.1">
        <property type="nucleotide sequence ID" value="XM_018854025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJI2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJI2"/>
    <dbReference type="GeneID" id="30027001"/>
    <dbReference type="OrthoDB" id="1594986at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120171">
        <property type="term" value="C:Cdc24p-Far1p-Gbetagamma complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000935">
        <property type="term" value="C:division septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120157">
        <property type="term" value="C:PAR polarity complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000282">
        <property type="term" value="P:cellular bud site selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072697">
        <property type="term" value="P:protein localization to cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13246">
        <property type="entry name" value="PH_Scd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010481">
        <property type="entry name" value="Cdc24/Scd1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033511">
        <property type="entry name" value="Cdc24/Scd1_PH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053026">
        <property type="entry name" value="CDC42_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47339:SF1">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06395">
        <property type="entry name" value="CDC24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15411">
        <property type="entry name" value="PH_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="263"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="464"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="PB1" evidence="4">
        <location>
            <begin position="698"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="624"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="675"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA23998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="791" mass="88723" checksum="20847A9A2F570AD0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK36</accession>
    <name>A0A1A0HK36_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Na+/solute symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_10528</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714866.1">
        <property type="nucleotide sequence ID" value="XM_018853791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK36"/>
    <dbReference type="STRING" id="869754.A0A1A0HK36"/>
    <dbReference type="GeneID" id="30026767"/>
    <dbReference type="OrthoDB" id="6132759at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015606">
        <property type="term" value="F:spermidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015204">
        <property type="term" value="F:urea transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11476">
        <property type="entry name" value="SLC5sbd_DUR3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Urea active transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium/glucose cotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DUR"/>
    </dbReference>
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        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46154:SF4">
        <property type="entry name" value="UREA ACTIVE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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        </location>
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    </feature>
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    </feature>
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        <location>
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    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="621"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA24385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="725" mass="78239" checksum="B18981313E7F36D4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK93</accession>
    <name>A0A1A0HK93_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_130646</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="OBA24307.1"/>
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    </dbReference>
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    <dbReference type="GeneID" id="30027274"/>
    <dbReference type="OrthoDB" id="4095240at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="281" mass="32041" checksum="DC28AE26A5B759FC" modified="2016-10-05" version="1">MIPKEPFLDDDINKYPRFILSAHHFKISSSAESFLEVNTQPPLARSTLRRLSREPYYHSNIGASRSTSSILLSYARPSDIFDYRYFDQLYLEDHEDVLVFDSDSDYGDSDYPDPNHCGTCEIDLFGKTPVRHTATLRFKSKIEHGKKLAHSNEISELGDHLVHMLTFKVNSRAQYTKPPRFWLSPSSDYFDQPQPRADLNFPTTSHHPRVAIPQCESTFVDSFLPPAQEDMSNNMNSSVGSKEKVDMPLAKQMLASDAYVTDHKPSTLRDDFGVATPIFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKW9</accession>
    <name>A0A1A0HKW9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_30723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714934.1">
        <property type="nucleotide sequence ID" value="XM_018855692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKW9"/>
    <dbReference type="GeneID" id="30028668"/>
    <dbReference type="OrthoDB" id="21418at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029196">
        <property type="entry name" value="HAPSTR1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645">
        <property type="entry name" value="CHROMOSOME 9, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38645:SF1">
        <property type="entry name" value="YALI0F12243P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15251">
        <property type="entry name" value="TAPR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291947" evidence="2">
        <location>
            <begin position="24"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="311"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="369" mass="39256" checksum="A7DE668E6297FD01" modified="2016-10-05" version="1">MKKIAWACLSCLALSCPVLPCLALRLSTTGRGGRRCGRTPGPIAPHQKPVAKEHPRHLPFSTPPPTQRATLRRASVEKNPHARQTPTTAEPTNRRANKLPRPQNRRGPKPAEAPEPPQRGFALPSSAHGTMDLSNLSSTLPPTKSLELVSLGTLTGELTDEFKNAAKSVAALYNARPQKSDRGPGATDVQAEFATAAKAVASLYRLGTSSGVLLMHKGYLECLDELLAEIASGEDIENWALTKRAELTNLYTHKDAARAGPPAAALEAPGLAGAGDHEFTFPPELASHTPFRPGVPPLSVSFHAKHRKAPGRAVPAAAPAAQLDADSDASDLGSDRDARRKRRAARAQQDAGKRRRRDVAKREAPGGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYZ4</accession>
    <name>A0A1A0GYZ4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_48029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA16994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709291.1">
        <property type="nucleotide sequence ID" value="XM_018858251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYZ4"/>
    <dbReference type="STRING" id="869754.A0A1A0GYZ4"/>
    <dbReference type="GeneID" id="30031227"/>
    <dbReference type="OrthoDB" id="6362633at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA16994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="216" mass="24207" checksum="6B9305683A08345E" modified="2016-10-05" version="1">MSVHVRSVEQLADLVTSDLQGKARLLVCIAGVPGAGKSTMVEKIIEHLRTKNVVSKALPQDGYHYYRRELEQFEDPQEAFRRRGAPFTFNAEAFVSSVRKLRTHATVTAPLFDHSKKDPSEGAIVIGPEVQVVFIEGNYVGLKDEPWAELDAATDRLWMIQQDPGLVRERLIQRHVSSGVCRSYEDAVERCDGLDWQNAVYVMENSRAPDVVVTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ13</accession>
    <name>A0A1A0GZ13_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_80182</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709311.1">
        <property type="nucleotide sequence ID" value="XM_018859188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ13"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ13"/>
    <dbReference type="GeneID" id="30032164"/>
    <dbReference type="OrthoDB" id="2444812at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="13"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA17014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="227" mass="24187" checksum="C5C7478264B0550A" modified="2016-10-05" version="1">MALDPKNDPLKEPVTPPGWRAKFDDKYNTWFYVNLDTKKSQWEAPAGSRFPADDIDKIEPPAYSPSQGKSAATDQRNASQNMSGSQYQASAQDTSRQQGYGGYSQQQPNYGGYPQQGYGGYPAQGGYMQPGYGGYPQPGYGQYPQPYPQQYPPQARQQKNSRFGGGSGMAMGAGAGLLGGLLISDAIHDGQMDAYEQGYDNGYENGDNGGGDFGGGDFGGGDFGGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ39</accession>
    <name>A0A1A0GZ39_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">XLF-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_17472</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA16993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709290.1">
        <property type="nucleotide sequence ID" value="XM_018854716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ39"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ39"/>
    <dbReference type="GeneID" id="30027692"/>
    <dbReference type="OrthoDB" id="4094682at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015381">
        <property type="entry name" value="XLF-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09302">
        <property type="entry name" value="XLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="XLF-like N-terminal" evidence="2">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA16993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="186" mass="21387" checksum="35B48D672DFFB5FD" modified="2016-10-05" version="1" fragment="single">LPLVWNQTLMDNKYYMFGFSRFALCTFYVTDLVLVWADTPATEDIYKKALERGISGFDEEKRSILMCNLAAAVSESPSHIRFSCPSLQTIAAEVKLSENLSWSFNADLCDSRVSSDFFRGLCCQSLANHGLSLYRGFQMENMIRTRDEYILYLEENYKTVNGTELMSKYRRQHAGSASILLRYSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ44</accession>
    <name>A0A1A0GZ44_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_48046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA16998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709295.1">
        <property type="nucleotide sequence ID" value="XM_018858253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ44"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ44"/>
    <dbReference type="GeneID" id="30031229"/>
    <dbReference type="OrthoDB" id="2357318at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="743" mass="85897" checksum="8C6EF83D3FE65E3C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1P1</accession>
    <name>A0A1A0H1P1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FYV8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_75327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709341.1">
        <property type="nucleotide sequence ID" value="XM_018858429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1P1"/>
    <dbReference type="GeneID" id="30031405"/>
    <dbReference type="OrthoDB" id="4081733at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA17946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="816" mass="91171" checksum="C8AD82974A317019" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1V9</accession>
    <name>A0A1A0H1V9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2,4-dienoyl-CoA reductase [(3E)-enoyl-CoA-producing]</fullName>
            <ecNumber evidence="3">1.3.1.124</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_80062</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a (2E,4E)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:45912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85101"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a (2E,4Z)-dienoyl-CoA + NADPH + H(+) = a 4,5-saturated-(3E)-enoyl-CoA + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:61892"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85099"/>
            <dbReference type="ChEBI" id="CHEBI:85493"/>
            <dbReference type="EC" id="1.3.1.124"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.124" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709343.1">
        <property type="nucleotide sequence ID" value="XM_018859166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1V9"/>
    <dbReference type="STRING" id="869754.A0A1A0H1V9"/>
    <dbReference type="GeneID" id="30032142"/>
    <dbReference type="OrthoDB" id="2136131at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008670">
        <property type="term" value="F:2,4-dienoyl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05369">
        <property type="entry name" value="TER_DECR_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045017">
        <property type="entry name" value="DECR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43296:SF2">
        <property type="entry name" value="PEROXISOMAL 2,4-DIENOYL-COA REDUCTASE [(3E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00026117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA17948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="30134" checksum="7F8513BA79C35AF1" modified="2016-10-05" version="1">MASLDRSFLDTCPWKPGIFKGKVAFVTGGAGTICRVQTEALVLLGADAVIVGRNTEKTERAARDIQRLRQGATVLGIGSTDVRDVHSVAAAVETAVRRLGRIDFVIAGAAGNFLADFNHLSANAFKSVVSIDLLGSFNTVKACFVELRKSRGAVLFVSATLHYYGVPFQAHVGAAKAGVDALSNALAVELGPLGIRCNCIAPGGIEGTEGMSRLMTADTDVGRKVPLARWGAARDIAEATVYVFSPAASYVTGTVQVVDGGFWHMGNPAADVYPGVVAAQNSLENPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4H8</accession>
    <name>A0A1A0H4H8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spindle assembly checkpoint component MAD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_47316</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709481.1">
        <property type="nucleotide sequence ID" value="XM_018858149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4H8"/>
    <dbReference type="STRING" id="869754.A0A1A0H4H8"/>
    <dbReference type="GeneID" id="30031125"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05557">
        <property type="entry name" value="MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="105"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="221"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="296"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="391"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="534"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA18946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="670" mass="77593" checksum="03077DA222125F79" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4X6</accession>
    <name>A0A1A0H4X6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ASTRA-associated protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ASTRA complex involved in chromatin remodeling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat ASA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709497.1">
        <property type="nucleotide sequence ID" value="XM_018856344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4X6"/>
    <dbReference type="STRING" id="869754.A0A1A0H4X6"/>
    <dbReference type="GeneID" id="30029320"/>
    <dbReference type="OrthoDB" id="7668193at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="64"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="428" mass="48281" checksum="5B659CA610A0D756" modified="2016-10-05" version="1">MYALSKHLASVRTQNYPMLPKFSLRAHKAPLTCLTCHQDKLVSSDRDGFIIIWNLTSRRPMALWRAHDGHILTLKLSPMGLISHGRDSMIRIWDLSLEYLKTCSENMSDIAALGAKLNPLLPQPIHAEIPVNALNFCNVDFCDGMMITPATVDSDNFDIYEVSDPFGLFNFKRVLQNISPLSLLRSEHLIEEIGASQHQDKRGGNGVIMRTLFVERSLFFVGYESGAIYGFSIEQNNHDKASVKERSIIFKDCNLSIVFSLHEHNPLPILSLVLNSATSELYTGSASKKLICYNVRNLLDIKFKQDENTSNFTPHNLRHYGIQCIQITNKMILTGFWDGVIKGFTNDFEEATRLERKEESIRPEHDGEISKPAKKSICMEIWAPCFQNSSVSCFNGRKNTLRRAATGKDTLLFVGYGDGLITAFKGIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H595</accession>
    <name>A0A1A0H595_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_13839</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat WDR48 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709628.1">
        <property type="nucleotide sequence ID" value="XM_018854434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H595"/>
    <dbReference type="STRING" id="869754.A0A1A0H595"/>
    <dbReference type="GeneID" id="30027410"/>
    <dbReference type="OrthoDB" id="2421129at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051246">
        <property type="entry name" value="WDR48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021772">
        <property type="entry name" value="WDR48/Bun107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19862:SF14">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11816">
        <property type="entry name" value="DUF3337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="153"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="247"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118016" checksum="A5AEDAAD93433DC7" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5D6</accession>
    <name>A0A1A0H5D6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_219342</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709665.1">
        <property type="nucleotide sequence ID" value="XM_018855119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5D6"/>
    <dbReference type="STRING" id="869754.A0A1A0H5D6"/>
    <dbReference type="GeneID" id="30028095"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF315">
        <property type="entry name" value="TRANSPORTER MCH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="348"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="481" mass="52285" checksum="50A82C43BC1D5106" modified="2016-10-05" version="1">MNAMAKTSSENLAVISKPLNDPTICDSELGSEEKRQCEDHEKDKVISAQTLAEIPDGGYGWFIVLACFLLNFNTWGANSGFAVYLSTYLNEDTFDGASKYDYALIGGMTFGVGLVFAPVVNWTQGKLGIQLTIILGMCFQFAGLMLASFSKKLWQLYLTQGMLQAFGLAFMSIPAMAILPQWFKKKRTFAAAISAAGSGCGGIMYNLAMQKVIEIQSVSWALRAQSILCFGLSWISIALIRTRMQVKFSLFDVSVLKTAGFYIATFYVVFCMLGYVVVLYNMANFTTSLGYSEYQGSIASAMVQVGSVLGRPVVGRISDVFGAITVTCAAYFLSAVFVFALWVPAKNYAMIIVFCIIEGSLMGLIFATIAPICAKLFGLKRVNVSLGMLWIFLGFSGIASPVIGLSLKTGGTGYVGSDQYLHCSIFSGAAFVACFATLLIMRGYVIHRQEMVTLDADEGHMHVTVPFLAPFYCALKPVAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5N8</accession>
    <name>A0A1A0H5N8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vhr1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_79797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709802.1">
        <property type="nucleotide sequence ID" value="XM_018859128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5N8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5N8"/>
    <dbReference type="GeneID" id="30032104"/>
    <dbReference type="OrthoDB" id="4089008at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007147">
        <property type="entry name" value="TF_Vhr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04001">
        <property type="entry name" value="Vhr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="601" mass="67975" checksum="254553ADC69E0F0A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5X3</accession>
    <name>A0A1A0H5X3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP cyclohydrolase 1</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GTP cyclohydrolase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_13647</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709887.1">
        <property type="nucleotide sequence ID" value="XM_018854402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5X3"/>
    <dbReference type="STRING" id="869754.A0A1A0H5X3"/>
    <dbReference type="GeneID" id="30027378"/>
    <dbReference type="OrthoDB" id="4966at2759"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00642">
        <property type="entry name" value="GTP_cyclohydro1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.286.10:FF:000003">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1130.10:FF:000012">
        <property type="entry name" value="GTP cyclohydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00223">
        <property type="entry name" value="FolE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018234">
        <property type="entry name" value="GTP_CycHdrlase_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00063">
        <property type="entry name" value="folE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006825">
        <property type="entry name" value="PRK09347.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006826">
        <property type="entry name" value="PRK09347.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109:SF7">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="GTP CYCLOHYDROLASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00859">
        <property type="entry name" value="GTP_CYCLOHYDROL_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="13">
        <location>
            <begin position="90"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="270" mass="30506" checksum="89EFC56E77166734" modified="2016-10-05" version="1">MSDGTIDPQAEQIADPTPKELVQPATKKAKRSPAEEVVAREFGTPLQTRAASPLTLNPPIDNDGLSWPSKGARQRIEQTPEEAKQRETRIANAVKTILTELGEDVDREGILETPERYARAMLYFTKGYEDNIRDVIKRAVFEEDHDEMVIVRDIEIYSLCEHHLVPFFGKAHIAYIPNKRVVGLSKLARLAEMYLRRFQVQERLTKQIAMALSEILKPRGVAVVIEATHMCMVSRGVQKTGSLTTTSCMLGCFRVQQKTRDEFLTLLGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H659</accession>
    <name>A0A1A0H659_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709935.1">
        <property type="nucleotide sequence ID" value="XM_018856332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H659"/>
    <dbReference type="GeneID" id="30029308"/>
    <dbReference type="OrthoDB" id="4010679at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="244" mass="28490" checksum="A05DDB1CDDC40DBF" modified="2016-10-05" version="1">MGIFFVALLLVLLPISLMTYLSFYRMLIPVERVQTPTKFTEIGPIMKPSLITDIDITGVRAFLQANDNLTFLVRLNLDAICKYETAHQSLTYSFNLCPELKVQDWLLVNCDSRYIYVEKNNWVPYNLRYWVPPIFVDVLKLVRVDHPLIQLKGHEILAALLGNKASLTLEKMNFLFLDSRKTHLDFVIEWDGLRYYLVNYYYSSLFIGAGSFWVLSSFFCIVSAVAFLQRFLREEEDIRTKKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H662</accession>
    <name>A0A1A0H662_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1237-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_46193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709973.1">
        <property type="nucleotide sequence ID" value="XM_018858003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H662"/>
    <dbReference type="STRING" id="869754.A0A1A0H662"/>
    <dbReference type="GeneID" id="30030979"/>
    <dbReference type="OrthoDB" id="7771656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008313">
        <property type="entry name" value="GH125"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31047:SF0">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 157 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06824">
        <property type="entry name" value="Glyco_hydro_125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028846">
        <property type="entry name" value="UCP028846"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01149">
        <property type="entry name" value="DUF1237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="64194" checksum="F5854C229B5C0DC1" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H665</accession>
    <name>A0A1A0H665_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709940.1">
        <property type="nucleotide sequence ID" value="XM_018856335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H665"/>
    <dbReference type="STRING" id="869754.A0A1A0H665"/>
    <dbReference type="GeneID" id="30029311"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="607" mass="69423" checksum="875010B7D75BBA16" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6C3</accession>
    <name>A0A1A0H6C3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF1742-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_46216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710038.1">
        <property type="nucleotide sequence ID" value="XM_018858009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6C3"/>
    <dbReference type="GeneID" id="30030985"/>
    <dbReference type="OrthoDB" id="2158714at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013640">
        <property type="entry name" value="Vfa1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28218:SF1">
        <property type="entry name" value="VPS4-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08432">
        <property type="entry name" value="Vfa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="208" mass="23390" checksum="57CC26FBF3CF35BC" modified="2016-10-05" version="1">MSAPPFPNEYRCRLVADSDSKPCTICFKPTSTVLLASNKADHFYVCPMHMKDKSFASPIILEDYTKLMSEKTRLENEVRLANSLADANRPYSWTKLMSNIGWSDPVQPRKENSEDKPKLDDAESSQAKKQKQDTYEELVSQANSLKTKLADVNSSIAGFQFKHYALAKDVYKMRVNSSLQGRNRVQKQSPGEASKSVVFPSVPKSDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6G3</accession>
    <name>A0A1A0H6G3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710025.1">
        <property type="nucleotide sequence ID" value="XM_018856239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6G3"/>
    <dbReference type="GeneID" id="30029215"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008508805" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14866" checksum="BF58AD1768D34A8A" modified="2016-10-05" version="1">MKFCSTIALLALISGSFASLGGGYFSIETVTGVQLKLFLSKLDKIIYAGEEISAYVIENGLVKAYLTMNANGRLVMAQGRDRNFLVDVSKGDLDGYLSHKENEIFCLRKTEVFVSDSGCQNALEVKIRFWKMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6R5</accession>
    <name>A0A1A0H6R5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33247</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710130.1">
        <property type="nucleotide sequence ID" value="XM_018856254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6R5"/>
    <dbReference type="STRING" id="869754.A0A1A0H6R5"/>
    <dbReference type="GeneID" id="30029230"/>
    <dbReference type="OrthoDB" id="3357846at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015244">
        <property type="term" value="F:fluconazole transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF23">
        <property type="entry name" value="FLUCONAZOLE RESISTANCE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="517"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="108"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="555" mass="61902" checksum="E326EC7A86E7CBF3" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6T8</accession>
    <name>A0A1A0H6T8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hsp90 chaperone protein kinase-targeting subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_79626</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710151.1">
        <property type="nucleotide sequence ID" value="XM_018859099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6T8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6T8"/>
    <dbReference type="GeneID" id="30032075"/>
    <dbReference type="OrthoDB" id="440202at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071474">
        <property type="term" value="P:cellular hyperosmotic response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071852">
        <property type="term" value="P:fungal-type cell wall organization or biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030474">
        <property type="term" value="P:spindle pole body duplication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.610">
        <property type="entry name" value="Cdc37, Hsp90 binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004918">
        <property type="entry name" value="Cdc37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013873">
        <property type="entry name" value="Cdc37_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013874">
        <property type="entry name" value="Cdc37_Hsp90-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038189">
        <property type="entry name" value="Cdc37_Hsp90-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800">
        <property type="entry name" value="CDC37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12800:SF4">
        <property type="entry name" value="HSP90 CO-CHAPERONE CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08564">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08565">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01069">
        <property type="entry name" value="CDC37_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01070">
        <property type="entry name" value="CDC37_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01071">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101391">
        <property type="entry name" value="Hsp90 co-chaperone CDC37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 Hsp90 binding" evidence="8">
        <location>
            <begin position="204"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Cdc37 C-terminal" evidence="7">
        <location>
            <begin position="423"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA19623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="530" mass="60732" checksum="22508AE538387694" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Y7</accession>
    <name>A0A1A0H6Y7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_208005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710321.1">
        <property type="nucleotide sequence ID" value="XM_018855009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Y7"/>
    <dbReference type="GeneID" id="30027985"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="81" mass="9475" checksum="5E94389986D34DA9" modified="2016-10-05" version="1">MRTSLLDYHIYYHSSLLKFPSLMYMCTRTSQSGFAIDRESKTPQSGTRLEWTRVRILGFQSPGPLAFLLVQIIYVFPRSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H705</accession>
    <name>A0A1A0H705_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_208561</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710341.1">
        <property type="nucleotide sequence ID" value="XM_018855016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H705"/>
    <dbReference type="STRING" id="869754.A0A1A0H705"/>
    <dbReference type="GeneID" id="30027992"/>
    <dbReference type="OrthoDB" id="306876at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF6">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="21"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="7">
        <location>
            <begin position="193"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="374" mass="40794" checksum="0FFFA0199D9B0C86" modified="2016-10-05" version="1">MDATKVRVSRFYYDVVTPNLGVSLLLTSQFLNTIMILTCKLLEVDPGLDTPIHPVQILFVRMLITYACCVLYMVTTKRVPDAPFGPKGQRTLLFLRGFLGFFGVFGLYYSLQYLSLSDAVTITFLIPMVTAFFAWIVLGEKYSLLEAVCSLLSLGGILLVAKPDFLFGLQSESETPQLDSAESSNSGLRVLGTVVGLLGVCGASAVYVLLRKIGKAAHPLISVSYFALTTCVICFCATILVPSLSFKAPANARQWFLFSLIGLSGFFMQFCLAAGLQLEKAGKSSLMIYSNMVFALVWDFVFWGHVPGMLSILGTSLILANAYIIIKYKPSSDAGDSTDLDMEANGGKYDAPGEIDMDFLISDEDEGEDEGSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H1</accession>
    <name>A0A1A0H7H1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Replication factor A protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_45247</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710367.1">
        <property type="nucleotide sequence ID" value="XM_018857898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H1"/>
    <dbReference type="STRING" id="869754.A0A1A0H7H1"/>
    <dbReference type="GeneID" id="30030874"/>
    <dbReference type="OrthoDB" id="188186at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="106" mass="11244" checksum="538ACBEE78B59B37" modified="2016-10-05" version="1">MNAASIRVNASTVKDFPGRVVRVIGKATSVDNYSNTATLDAGGPIDVSTLSSDPLEVGKIYEVVGKCNVNDLKVSSYSVTQLSDNVNLEMAAKLASMVPKVSELFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H9</accession>
    <name>A0A1A0H7H9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">37S ribosomal protein S25, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79261</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the mitochondrial small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710460.1">
        <property type="nucleotide sequence ID" value="XM_018859025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7H9"/>
    <dbReference type="GeneID" id="30032001"/>
    <dbReference type="OrthoDB" id="5542239at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016939">
        <property type="entry name" value="Ribosomal_mS23_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37799">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S25, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37799:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13741">
        <property type="entry name" value="MRP-S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029764">
        <property type="entry name" value="RSM25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="227"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR029764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="266" mass="30830" checksum="2AF1A2A5F36D9C62" modified="2016-10-05" version="1">MKIQTKASQVLERTSHYIKAGIIRDKPAWYDAVAAVPPTFNMEKLPKNLGPNQLQDPMEQLWKEQGGYIKTRASAPERAQKNNFVSRVPKLQFLEDQLRDVFYHQHPWEFSRPKTLIENDGDDQAKCDWSRMLQLNKPLDGESVVQRTLWLLQDSKQQQKEKTLFEAYDQARFEFYQLRMAEEMNSVVSKEESLMFGAHFASSNLQWGVQKEQETIDRWAKIGEKRTKIREASRSKASASAGIAATPEEPTNMWESFLSSEEPTNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7I3</accession>
    <name>A0A1A0H7I3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1</fullName>
            <shortName evidence="8">Oligosaccharyl transferase subunit WBP1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32986</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the DDOST 48 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710511.1">
        <property type="nucleotide sequence ID" value="XM_018856181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7I3"/>
    <dbReference type="STRING" id="869754.A0A1A0H7I3"/>
    <dbReference type="GeneID" id="30029157"/>
    <dbReference type="OrthoDB" id="29105at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005013">
        <property type="entry name" value="DDOST_48_kDa_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055459">
        <property type="entry name" value="OST48_MD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055457">
        <property type="entry name" value="OST48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10830:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE 48 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23358">
        <property type="entry name" value="OST48_MD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03345">
        <property type="entry name" value="OST48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008444429" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1" evidence="8">
        <location>
            <begin position="22"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 N-terminal" evidence="9">
        <location>
            <begin position="53"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="OST48 middle" evidence="10">
        <location>
            <begin position="269"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="440" mass="49284" checksum="8753E65049B29C66" modified="2016-10-05" version="1">MFSSAIAKLVAIGAVFCLATAANSADSLKPHVLVAYDSSLVPDLEVLEKSILLDSNKYEVNYLDYLKKDEELLVTDEARYDHAIFLPSSKKVVEAKGLVDKHKLLEFFNLGGNIIAVGSSDYSVPEEVRLLLAQVGIHPAPRGFTLSDHFDTNVRINDENLVSKKIISQIKDLNYEGTAALVSNSQYLFPLVKAPKFSFTSSSKDKMLTSEKTWTVGEQGFLAVAFQGLNNARGAWVGSLDLLSDDLTSWVLQEKGVLRLNFVEHYKQDEPGASNRTLYRIKDSVYYTVGVSELNNGEWVPYTPSSEDDVLQLSFKMLDPYQRLNMTLLGPAASTENGVNDINVFFVEFVIPDHHGMFTFELDYKREGLSFLEDKKIVAVRHLANDEYKRSWEITNAWMYMVSAALVVIAWLIFVAGFLYLGVPKKIDSSKETQKKVAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7J8</accession>
    <name>A0A1A0H7J8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RPS0</name>
        <name evidence="11" type="ORF">METBIDRAFT_45616</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes).</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with RPS21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710526.1">
        <property type="nucleotide sequence ID" value="XM_018857936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7J8"/>
    <dbReference type="STRING" id="869754.A0A1A0H7J8"/>
    <dbReference type="GeneID" id="30030912"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000010">
        <property type="entry name" value="40S ribosomal protein S0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03015">
        <property type="entry name" value="Ribosomal_S2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027498">
        <property type="entry name" value="Ribosomal_uS2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00962">
        <property type="entry name" value="RIBOSOMAL_S2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="214"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="226"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="267" mass="29374" checksum="665A9704F2281059" modified="2016-10-05" version="1">MSLPESFALTAEDAKLLLAANVHLGSKNVQVHNKPYVYKTRPDGVNVIDISKTWEKIVLAARVIAAIPNASDVAVCSSRTFGQRAVLKFAAHTGATPIAGRFTPGNFTNYITRSFKEPRLVMVTDPRTDAQAIKESSYVNIPVIALSDVDSPSEYVDVAIPCNNKGKHSIGLIWWMVAREVLRLRGIIPDRESEWTVMPDLYFYRDPEEIEQNAAEEARGTTTTTEETEEAVAEAETEWNTETNVEDWADSGVTAAGEEAAATQWSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7U4</accession>
    <name>A0A1A0H7U4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_45413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710694.1">
        <property type="nucleotide sequence ID" value="XM_018857916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7U4"/>
    <dbReference type="GeneID" id="30030892"/>
    <dbReference type="OrthoDB" id="4084459at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="254" mass="28654" checksum="0FF91E9A05C99602" modified="2016-10-05" version="1">MKRARLETMLENLTLQGNAETSRKPHFQVNPLIEPLEPEPKKRRTADLDTLILEKMAEAYKDHMLQGLAVVRYAHPVSVLVWHFQQWVKRLFNMFVRKYNQRPGLRNMRPFRDFGRIMALTRDANVAFTYRDLLGIVLEENAIETQRLLRKREDRLGCQRLQEIKDEEELARESSYTYWDRVSALSGDVSALSGDVNMDEYGSDGYGLEAWPPGGSLGAGADLDMAMAIDASGAECAMLANHGSPGASYAGALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7X0</accession>
    <name>A0A1A0H7X0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_12244</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710716.1">
        <property type="nucleotide sequence ID" value="XM_018854196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7X0"/>
    <dbReference type="STRING" id="869754.A0A1A0H7X0"/>
    <dbReference type="GeneID" id="30027172"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="19"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="49892" checksum="AAA485003D09427F" modified="2016-10-05" version="1">MALISPASTEDAPASVAGVPLRKIAVGGVSAEFPALLVDCYQYILDHGLVEGIFRVSGSIKRMRDVTANYAAYRDWLAHGSPSPHDVSGVAKKFTRDYLASMGGLFPPPVLAQLRALFAAPAPPGSTPSLESCRSTSTSCSSGLESVLEEAAENTYPAPRSVADTLDAVAVLLVSKNLARKNAVFIYYLSVLRCVLAHQDETRMPNANLAIVFQQYLFETWVVAELQLYQGLLKFLLDHYDTFVAKYRLSLALLGGIDEPAPAADAGSVHSSEPVSSSPLTEYSLHQASPPKFSVYGSPEGGRSSLSQKFTSLWEAYATPASRARRFSFMSRGSGRSCDRLPLDPDPLPTAGPACSKVTLAAAQHQVISESGEPFPGPSPQDTEPRQTAPGTTSRTSFMVFLEAANDLGAPPKWAKQPVRAEPAGPRAASHSPDSLPRDHNPPSFPGAAKRQSIGRRFSLWLHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7Z9</accession>
    <name>A0A1A0H7Z9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_44774</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710635.1">
        <property type="nucleotide sequence ID" value="XM_018857837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7Z9"/>
    <dbReference type="STRING" id="869754.A0A1A0H7Z9"/>
    <dbReference type="GeneID" id="30030813"/>
    <dbReference type="OrthoDB" id="2159384at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF46">
        <property type="entry name" value="PROTEIN ERD1 HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="203"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="427" mass="49127" checksum="4903864AD089448D" modified="2016-10-05" version="1">MIDRSLSPLTFDDVIPLPHRVAIMFQLGVFLFYSLVWVIYNGFLVNCLALLNFAYSSHKFSGDGHDVKVVTGEMATVSSAESSENMLLIMGIRKTFAKTFTFTSSGLVVYWTCLIVLPGSPILELAATWLPACLLLISLYTSFRLGDTWGQNRVNATLKRVLVGRINSATMRTNDILLSDTLTSYSKVINDFASLLWVMLMPGDITYNNKVEALVLSYPALLRMKQCWYEFKSTRQRQHLLNMCKYLALLGPVLINLLIKINMGLVSDADGSQTLNKLNRWWYVLLAFSSMYLFIWDIKMDWGFEAFDILFRLNVGEALLLRSPSKLVCKGYPGYYLVILFDFVIRFIWVFKVFVIKETEIKLRLQNHVGNFLFGYDYLSLGYAILEFLEIFRRWLWCFIKLESDLVKLQLRDDFARATPLATLKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H833</accession>
    <name>A0A1A0H833_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Long-chain-fatty-acid CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_33031</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710577.1">
        <property type="nucleotide sequence ID" value="XM_018856198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H833"/>
    <dbReference type="STRING" id="869754.A0A1A0H833"/>
    <dbReference type="GeneID" id="30029174"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="151"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="722" mass="80962" checksum="9C0CB4D2F2F2C940" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H858</accession>
    <name>A0A1A0H858_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arabinanase/levansucrase/invertase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_78660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710728.1">
        <property type="nucleotide sequence ID" value="XM_018858910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H858"/>
    <dbReference type="STRING" id="869754.A0A1A0H858"/>
    <dbReference type="GeneID" id="30031886"/>
    <dbReference type="OrthoDB" id="21615at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18610">
        <property type="entry name" value="GH130_BT3780-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007184">
        <property type="entry name" value="Mannoside_phosphorylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106">
        <property type="entry name" value="GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34106:SF5">
        <property type="entry name" value="GLYCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04041">
        <property type="entry name" value="Glyco_hydro_130"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="340" mass="38638" checksum="A64DE43381FBB8A7" modified="2016-10-05" version="1">MIFPDIGPHTLDGDPDYRPPTTFPIGPQFSKYAGNPILKPNPNNEFEEAYIYNAAAIVLGGRVFLLYRAQNKQLVLSVGLAWLDDGFHFKRWPEPILYPTEPWEMGGGCEDPRIVRDPESRKFIVTYTAYDRRLARLCVAESTDLLTWKKHPPVIPQDRWAEACVSLKGEPFVRHGWLKSGAIFVDRHKDGKYYMIWGESGFQLAVSDDLVHWTIRTQVYARGILDWQDRLIEPGPAPIKIDTGGKHNHYVLFYNSSTVGSGMYEKGTYTISQMLIDYDNLQDGPLARMDRPVLVPEEKNEVVGQVNRVVFTEGVVQFKGRWFLYFGAGDSELSVATCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8G0</accession>
    <name>A0A1A0H8G0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_25721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710833.1">
        <property type="nucleotide sequence ID" value="XM_018855300.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8G0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8G0"/>
    <dbReference type="GeneID" id="30028276"/>
    <dbReference type="OrthoDB" id="2553626at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="355"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="469" mass="50118" checksum="473E1488290B23DD" modified="2016-10-05" version="1" fragment="single">VPLSLTAQQLSPEEARTYMRWYLDILARANLRTIAISDVYQFLGNFRLAPATRAKINRVFSKILLLINIGEFFAVLRVVAHALQGREPARALIQTPTRVPRPPSILSQKRPSDDAADDTGRAGAVSAPLDIDLFTQFLLTGERPTDRGAAPPRKRKSVKFCDQLVSDIRATPAPAEPAWDDLLPMDQLMSRMHVAAAPAAGAGLLAVPWRNAAGASPDPEERQILRDMAPQMNHFRNLHSVDTILVGGVPATIHLPEHSDLFLPRNALPQPPLLRPNMTGPAQMAQMGPPPLRPNATGPHDMARFLSPPPPGAPRVSLQAFTSQMAGPTVDNTQQNARIGAVSPPPTPASRRARSASLPSPGPLAWDPPAGRNSAAFNPHLAAPAATPGPPVPARSLLGHAHGTPPPPPPRSRRAGSTSSPVPPPLPAKIPNAPLYQAPQGSSSTTDILDDLKALQEEVDKIRDLTGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8M0</accession>
    <name>A0A1A0H8M0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Autophagy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_44020</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in cytoplasm to vacuole transport (Cvt) and autophagic vesicle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Conjugated with ATG12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Preautophagosomal structure membrane</location>
            <topology evidence="1 7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the ATG5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710883.1">
        <property type="nucleotide sequence ID" value="XM_018857735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8M0"/>
    <dbReference type="STRING" id="869754.A0A1A0H8M0"/>
    <dbReference type="GeneID" id="30030711"/>
    <dbReference type="OrthoDB" id="272162at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044233">
        <property type="term" value="C:mitochondria-associated endoplasmic reticulum membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061908">
        <property type="term" value="C:phagophore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019776">
        <property type="term" value="F:Atg8-family ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.190">
        <property type="entry name" value="Autophagy protein Apg5, helix rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007239">
        <property type="entry name" value="Atg5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048940">
        <property type="entry name" value="ATG5_HBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042526">
        <property type="entry name" value="Atg5_HR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048939">
        <property type="entry name" value="ATG5_UblA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042527">
        <property type="entry name" value="Atg5_UblA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048318">
        <property type="entry name" value="ATG5_UblB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13040:SF2">
        <property type="entry name" value="AUTOPHAGY PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20637">
        <property type="entry name" value="ATG5_HBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20638">
        <property type="entry name" value="ATG5_UblA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04106">
        <property type="entry name" value="ATG5_UblB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="5 7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Autophagy protein ATG5 UblA" evidence="10">
        <location>
            <begin position="15"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 alpha-helical bundle region" evidence="9">
        <location>
            <begin position="130"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Autophagy protein ATG5 UblB" evidence="8">
        <location>
            <begin position="193"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="277" mass="31517" checksum="2AFD99AFC8FCAFEF" modified="2016-10-05" version="1">MSQQVTEKVWNGLVHVRVSYQDAEYLLEAKRMLYFPVYFPRIIDFFRIATGCLDLCAQPVWLEHGNVAIRWNLPVGLLYDLLYLPDHETARDRSWTLVLRLASPHNPYPSDQIIPFGPASGETVDYDTLLNQVFINALKQSCYVLNGNSRSVLSLSADDTCALWRAMAKHDMSVYSNIRRKLATPGQGTRHRLPLKIFEAGSATLFQAPVWSADTDGRPNTLARAMSAQVAGLSARPFPRVYVHGVNVDVLLNEPLVDLWTRFRFMDNFLYIVITRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8W3</accession>
    <name>A0A1A0H8W3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Alkaline phosphatase</fullName>
            <ecNumber evidence="3 15">3.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_78821</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 15"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710962.1">
        <property type="nucleotide sequence ID" value="XM_018858947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8W3"/>
    <dbReference type="STRING" id="869754.A0A1A0H8W3"/>
    <dbReference type="GeneID" id="30031923"/>
    <dbReference type="OrthoDB" id="7392499at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047386">
        <property type="term" value="F:fructose-2,6-bisphosphate 6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009166">
        <property type="term" value="P:nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.60.40:FF:000002">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000063">
        <property type="entry name" value="Alkaline phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 13 15" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Phosphoserine intermediate" evidence="12">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA20440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="490" mass="52752" checksum="299F06E6B5B605F3" modified="2016-10-05" version="1">MVTDGMGPASLSLARSFRQHRDGRPIDDLLYLDQHFIGTSRTRSNDSLVTDSAAGATAFSCGLKSYNGAIGVGPDKSPCGTILEALKLQGYLTGLVVTTRITDATPAAFSAHVDYRFQEDLIAEQQLGGYPLGRTVDLVLGGGRCHFLPSSVPGGCRADLRNLVAEAEASNYTYVGDRAAFDALASGENVTLPLLGLLAPTDIPYDIDRDARVHPSLAEQVRTALTALSKATADSAHGFFLLIEGSRIDHAGHHNDPAAQVREVLAYDDAYKAVLDFIDASDVATVAISTSDHETGGLVTARQVTPAYPDYLWYPQVLLNSTHSGEYLTRKILGKRRSYGPDLEAAAFSKFIAEEIVEADMGILDHTADEVAQIARLASSPERLLYFLNNMVSVRSQTGWTTHGHSAVDVNIYAYSNSEHINRQLYQRGAYLGLLGSHENIELGAFMETLTGVSLADVTELLRDTKHSPGVAVSDVAGLARAMFEHPLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H917</accession>
    <name>A0A1A0H917_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Very-long-chain 3-oxoacyl-CoA reductase</fullName>
            <ecNumber evidence="12">1.1.1.330</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">3-ketoacyl-CoA reductase</fullName>
            <shortName evidence="12">3-ketoreductase</shortName>
            <shortName evidence="12">KAR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Microsomal beta-keto-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_78857</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the microsomal membrane bound fatty acid elongation system, which produces the 26-carbon very long-chain fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty acid elongation. VLCFAs serve as precursors for ceramide and sphingolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA + NADP(+) = a very-long-chain 3-oxoacyl-CoA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="EC" id="1.1.1.330"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Endoplasmic reticulum membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.330" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711023.1">
        <property type="nucleotide sequence ID" value="XM_018858961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H917"/>
    <dbReference type="STRING" id="869754.A0A1A0H917"/>
    <dbReference type="GeneID" id="30031937"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045703">
        <property type="term" value="F:ketoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141040">
        <property type="term" value="F:very-long-chain 3-oxoacyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05356">
        <property type="entry name" value="17beta-HSD1_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000317">
        <property type="entry name" value="Very-long-chain 3-oxoacyl-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03107">
        <property type="entry name" value="3_ketoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027533">
        <property type="entry name" value="3_ketoreductase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086:SF2">
        <property type="entry name" value="HYDROXYSTEROID DEHYDROGENASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43086">
        <property type="entry name" value="VERY-LONG-CHAIN 3-OXOOACYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000126">
        <property type="entry name" value="11-beta-HSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 12" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0521">NADP</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA20501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="341" mass="36899" checksum="9ACD803E2061AC8C" modified="2016-10-05" version="1">MSNFIGSITESKLGQAALYTALLTGVYKLTTFTLSFGSLLLDLFVLPPTDFVKYGAKKGNWAVVTGASDGIGKEYAFQLASKGLNVVLVSRTLSKLEAIATEIQEKFGVKTEVIAFDASEDNEANYKAIQDTIADLPVTVLVNNVGQSHSIPVPFLETEQKELTDIITINNLVTLKITQTVAPVIVKTVAGQKKKVRGLILSMGSFGGLIPTPYLATYSGSKAFLQQWSSALAGELKPQGVDVQLVVSYLVTSAMSKIRRTTATIPNPKQFVKATLNMAGKRGGAQERFATSTPYWSHALMHFFIENSVGVYSSIGNKLNLDMHKSIRARALKKAARKKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H925</accession>
    <name>A0A1A0H925_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_12400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710904.1">
        <property type="nucleotide sequence ID" value="XM_018854224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H925"/>
    <dbReference type="GeneID" id="30027200"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="147" mass="17241" checksum="07A575230189FACB" modified="2016-10-05" version="1">MYRNIDFKLTNLSYKTARHVLLGRKRFSKFETNGTFRSTLSCAYGNGVLYCLKIMSSKTVDTKRNFAQHKENFKICLKSKIASWLPQLFSFDKDRTNITRNTADQWFSDIVDFLHLPIEEGDDEEDKEPDNQNYDELGLNNNHTWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H955</accession>
    <name>A0A1A0H955_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DUF1749-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43058</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711052.1">
        <property type="nucleotide sequence ID" value="XM_018857614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H955"/>
    <dbReference type="GeneID" id="30030590"/>
    <dbReference type="OrthoDB" id="10034502at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013744">
        <property type="entry name" value="SidJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591:SF1">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08538">
        <property type="entry name" value="DUF1749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="306" mass="33955" checksum="5763889BF5E2312A" modified="2016-10-05" version="1">MHSVVIHNYSGNLSAIEFGEKNTIPHNFLVFIGGLGDGFLSVPYVPPLAEAITQRFGKRWVLVQALISSSYLGFGTGSLRRDCKELSKLIKYLRTTRGSKDSKVILMGHSTGSQDTLEYMSKYSFSGDCPTDLNIDAGILQAPVSDSEGLKETFGEDQNLIALIAKSQALIDDGKPNELMPASCLKYTFGSPVTAYRFHSLLSQRGDDDYFSSYLHETDLKKSFGHVLRPILVLYSGDDEFASSDLDKNQLLVRWRAACDPKYWSKHSKIVEGATHNVGKHSKIGALDQLIKSVLSFVEDEFERVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H994</accession>
    <name>A0A1A0H994_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711211.1">
        <property type="nucleotide sequence ID" value="XM_018856106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H994"/>
    <dbReference type="GeneID" id="30029082"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="103" mass="11713" checksum="9D1290248F05AD1D" modified="2016-10-05" version="1">MNHAYELVLVLVRPFQTISSLIHDYKYLVTEALTDLRMQSGNLFFPCTVGHPANQAFLATRTISVQQKILAWQSAYHTCAAPHLNQRLSKRTDLEPGHQPESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9H7</accession>
    <name>A0A1A0H9H7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_205754</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711301.1">
        <property type="nucleotide sequence ID" value="XM_018854981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9H7"/>
    <dbReference type="GeneID" id="30027957"/>
    <dbReference type="OrthoDB" id="4092725at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291700" evidence="2">
        <location>
            <begin position="25"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA20779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="472" mass="52837" checksum="258EF027EFFE410E" modified="2016-10-05" version="1">MFVLAVAAAFSAWLLFQTPAPTSQVQQARLLRDFVLANFSVAQPVHVDFGDRGFRFPDLVEAAQRQLDFRLAADGIPYRYPLRDRLPKVVPRPETELEAALARPPLLTDEGDGPSGGGGPSTAEDPVGAGSNVGEWTSVLGGSRDGDARGNSRDLEPSTLANPRHLEPSTLGRGLSNTSAAAPQTRVPEYSVEIYFQDLPRLSLDLVQLKAYMAYSLDFIHQNDLPFFLTQAIYDTLLQPDLDMRRQMLAGRCRLQDVTRHLNINFVAAEELGETRPEISAAILQHMRQFEAILAYVKIVPSVHVLNVLKHRATAGLLHNSSSELSFFYLTTMRPYANSVQNVPLRHLDPLAKEPLSEGNVYGTEYQRRLRLSQTDTYNITSFLEDATRAIKQAIRLPRDCSNLRLVAESVMKHYILGGLVEMLDLLIAQEPFDTRAYMHILRIVDDILLEETHDWASRLTEVMALHDKLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9T5</accession>
    <name>A0A1A0H9T5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="3 15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_32606</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 15"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711160.1">
        <property type="nucleotide sequence ID" value="XM_018856097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9T5"/>
    <dbReference type="STRING" id="869754.A0A1A0H9T5"/>
    <dbReference type="GeneID" id="30029073"/>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030875">
        <property type="term" value="C:rDNA protrusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033679">
        <property type="term" value="F:3'-5' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000011">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="491"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA20638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="904" mass="100746" checksum="8FF2F8AE4D5C92E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Z6</accession>
    <name>A0A1A0H9Z6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">G protein gamma domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711464.1">
        <property type="nucleotide sequence ID" value="XM_018857596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Z6"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Z6"/>
    <dbReference type="GeneID" id="30030572"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G protein gamma" evidence="1">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="85" mass="9765" checksum="D7C8FC51CF0AF934" modified="2016-10-05" version="1" fragment="single">RMDERILDLKIRRIEQLNEKLRLSLKKDRIPASRAAALIIQASQDIPDPLIPSIWHLPPELNRYRVFQEAKGMSSGKNVSCCTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA56</accession>
    <name>A0A1A0HA56_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_229487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711395.1">
        <property type="nucleotide sequence ID" value="XM_018855227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA56"/>
    <dbReference type="GeneID" id="30028203"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="551"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="575" mass="61190" checksum="98F58423DE8B04EF" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAL2</accession>
    <name>A0A1A0HAL2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Peroxisomal membrane protein PEX13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Peroxin-13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_177659</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="13">Interacts (via SH3 domain) with PEX14 (via SH3-binding motif); forming the PEX13-PEX14 docking complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peroxin-13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711679.1">
        <property type="nucleotide sequence ID" value="XM_018854733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAL2"/>
    <dbReference type="STRING" id="869754.A0A1A0HAL2"/>
    <dbReference type="GeneID" id="30027709"/>
    <dbReference type="OrthoDB" id="10037838at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016560">
        <property type="term" value="P:protein import into peroxisome matrix, docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11771">
        <property type="entry name" value="SH3_Pex13p_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000128">
        <property type="entry name" value="Peroxisomal membrane protein (Pex13)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007223">
        <property type="entry name" value="Peroxin-13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035463">
        <property type="entry name" value="Pex13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19332:SF1">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04088">
        <property type="entry name" value="Peroxin-13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="316"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA21169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="414" mass="44160" checksum="579878BD689B2440" modified="2016-10-05" version="1">MSVPRTKPWETSSGTSAASNAATDSPGSLGTSSAIIPERPTSILEQDNTMGFGAGGMSSSYGAGNYGGGYGNSRYSGGYGSSMYGSGYGSSMYGSGYGSGMYGGYGGYGSSMHGGYGSGMYGSSFGGGMYGQNGMNGMNGMNGPGGLAEGTQATFQLIESIIGAVGGFAQMLEATYMATHSSFFTMVSLAEQFGNLKNTLGSLLGIFAVIKFAKRIFFKITGRPYNHGISLKEFSKFELKQKKLEDKIRRQQASAGKPARISFKPLLVFLAASIGFPYLLSKAIQTLNMQQQRQQQRRLQDQQGMAAGTRGPIDPESLEFAKALYEFNPENPNIEIELKAKELVAILSKLDPLGNESKWWKVRSRTGKVGYVPLNFLSIIERKSVKQVEAAPQITPVNVDSSKKVSFADGFSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAX1</accession>
    <name>A0A1A0HAX1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_11620</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711543.1">
        <property type="nucleotide sequence ID" value="XM_018854036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAX1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAX1"/>
    <dbReference type="GeneID" id="30027012"/>
    <dbReference type="OrthoDB" id="4096720at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="387"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="546"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="801"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="806"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="833"/>
            <end position="844"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="844" mass="94735" checksum="E192880903530C3F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAX7</accession>
    <name>A0A1A0HAX7_9ASCO</name>
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        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31756</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711676.1">
        <property type="nucleotide sequence ID" value="XM_018855909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAX7"/>
    <dbReference type="STRING" id="869754.A0A1A0HAX7"/>
    <dbReference type="GeneID" id="30028885"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF29">
        <property type="entry name" value="AMINO ACID TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="422"/>
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            <begin position="428"/>
            <end position="451"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="571" mass="62560" checksum="C347B29A2F6CB47A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAZ3</accession>
    <name>A0A1A0HAZ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Securin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_172200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711562.1">
        <property type="nucleotide sequence ID" value="XM_018854698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAZ3"/>
    <dbReference type="GeneID" id="30027674"/>
    <dbReference type="OrthoDB" id="4093331at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="319" mass="35453" checksum="BB682FAB43E3D511" modified="2016-10-05" version="1">MSTNRLKLHTDKENEHKIALAKNELRNPAHREIPAGTSVPTKRPLSAKSNINEKRVRVPLGGKNHNQAFPGLQRSKLSLPLTFQTPSLRQPGLTLQGDILSTKPTLTKSNLSLGFFNRVAKPSAPAPRPTNPHKNTLFPLQDLHRPKDLIPRFLSDDLIKAAPKALHPLQTSLNETLSDATAQLHASNVPSQEHFAKNRDPIKKGTFRPEIELLIEALAEDENSIETVPEAENREPLDYVPLGVSPLSKAEAPFLYEEEAPRKLTDMFAPDEQFFSDDEEQCSDAEKELNTAFYRELNGTEGNTALGLTAADLEDLLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB44</accession>
    <name>A0A1A0HB44_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">STAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31784</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711720.1">
        <property type="nucleotide sequence ID" value="XM_018855916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB44"/>
    <dbReference type="STRING" id="869754.A0A1A0HB44"/>
    <dbReference type="GeneID" id="30028892"/>
    <dbReference type="OrthoDB" id="427213at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="7">
        <location>
            <begin position="334"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="460"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="464"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="513" mass="57145" checksum="6551D26A855C80B1" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB51</accession>
    <name>A0A1A0HB51_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Elongation factor 1-alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_39991</name>
        <name evidence="11" type="ORF">METBIDRAFT_41977</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711617.1">
        <property type="nucleotide sequence ID" value="XM_018857475.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713050.1">
        <property type="nucleotide sequence ID" value="XM_018857216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB51"/>
    <dbReference type="STRING" id="869754.A0A1A0HB51"/>
    <dbReference type="GeneID" id="30030192"/>
    <dbReference type="GeneID" id="30030451"/>
    <dbReference type="OrthoDB" id="3969558at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000211">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9 11" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="5"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="458" mass="49829" checksum="ED4453BC2C98298A" modified="2016-10-05" version="1">MGKEKSHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAYTLGVRQLIVAVNKMDSVKWDKNRFEEIIKETSNFVKKVGYNPKTVPFVPISGWNGDNMIEASTNCPWYKGWEKETKAGKSTGKTLLEAIDAIEPPSRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVIKAGMVVTFAPAGVTTEVKSVEMHHEQLVEGLPGDNVGFNVKNVSVKEIRRGNVCGDSKQDPPKGAASFTAQVIVLNHPGQISSGYSPVLDCHTAHIACKFDTLLEKIDRRTGKSLESEPKFVKSGDAAIVKMVPTKPMCVEAFTDYPPLGRFAVRDMRQTVAVGVIKAVEKSDKAGKVTKAAQKAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBC2</accession>
    <name>A0A1A0HBC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_40791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711682.1">
        <property type="nucleotide sequence ID" value="XM_018857313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBC2"/>
    <dbReference type="GeneID" id="30030289"/>
    <dbReference type="OrthoDB" id="4084730at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012882">
        <property type="entry name" value="Fmp46"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07955">
        <property type="entry name" value="DUF1687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="229" mass="25593" checksum="0D5814327090AB84" modified="2016-10-05" version="1">MLRFARLITGSASPTITLFHNTNSKLSHHLLERLTIHKSRFLLDVRQNRLPLYETYSFIHQECMNVHPQNNRSFERIFPALLASPSHLFCNVEVKTKSKLKQFVPDLELVNESSYLDNIALHNATELSPFVVDWANKLVAVDDEGLDTIMQNYYSCGMQKSSKVHLPEGSDSIHQGPDSGNSPIFNSPKISLQETCGPQYNQSAAAAAAAATAARCVVHPHIAEFADLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBK8</accession>
    <name>A0A1A0HBK8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_78369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711906.1">
        <property type="nucleotide sequence ID" value="XM_018858871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBK8"/>
    <dbReference type="GeneID" id="30031847"/>
    <dbReference type="OrthoDB" id="4091087at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="33177" checksum="4AD58987550C822F" modified="2016-10-05" version="1">MRRFFSTLPAPAARFLAPPEKPALLVKPNPLITSKLCVKRDPVTSRAPVSWKDLPHIRTDVLPEEATDIRGIDTSGHQVLSQRRVIGKLPTLLSCNHFTLTFIPHNHTISSLRKSNVAYTFTNYGSPNLNDLNLLKRLTEYRGKYRFLEYFKSVHNPMGTAVSRSRFRKLIKRELHHALHQIIPNTQSEVEKVSGIFHFRFHMYPVPREADALREEVTNAVRRLYTNEGFRRSLALITQQQVQAFPNVYMLVRDIKVENAIGAQSAPGYFPKLPFLKPKTYRPRKIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBS8</accession>
    <name>A0A1A0HBS8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nucleoside hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_41524</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IUNH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711976.1">
        <property type="nucleotide sequence ID" value="XM_018857413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBS8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBS8"/>
    <dbReference type="GeneID" id="30030389"/>
    <dbReference type="OrthoDB" id="432381at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070635">
        <property type="term" value="F:nicotinamide riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070636">
        <property type="term" value="F:nicotinic acid riboside hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008477">
        <property type="term" value="F:purine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045437">
        <property type="term" value="F:uridine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006216">
        <property type="term" value="P:cytidine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034355">
        <property type="term" value="P:NAD+ biosynthetic process via the salvage pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019358">
        <property type="term" value="P:nicotinate nucleotide salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006152">
        <property type="term" value="P:purine nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008655">
        <property type="term" value="P:pyrimidine-containing compound salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006218">
        <property type="term" value="P:uridine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.245.10">
        <property type="entry name" value="Ribonucleoside hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001910">
        <property type="entry name" value="Inosine/uridine_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023186">
        <property type="entry name" value="IUNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036452">
        <property type="entry name" value="Ribo_hydro-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304">
        <property type="entry name" value="INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12304:SF4">
        <property type="entry name" value="URIDINE NUCLEOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01156">
        <property type="entry name" value="IU_nuc_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53590">
        <property type="entry name" value="Nucleoside hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine/uridine-preferring nucleoside hydrolase" evidence="4">
        <location>
            <begin position="14"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="354" mass="38349" checksum="B76337C1E6FD216F" modified="2016-10-05" version="1">MPSVPRRSPSAVPVWLDCDPGNDDAFAILLAAFHPSFRLCGISTVHGNVSLRKTTRNALALLEKLRFAQGEIPVYAGEPAPLVVPPLHAEAIHGSYGIGGTDLPETPGLQANTEMPYTEAMARAVEDHAGELCIVCTGALTNLVKFMARFPGLVPKIRLVSIMGGAFDCGNISPGVEFNMRTDPHAAQRVFAAPGLANKILLAPLNLTHTALATPAVRLAIYNPLGKNSSALRKMFSQILTFYNNAYVKKYGLHQGPPIHDPLALFLLLPKLASEAPELLEYAESCDFHYLQRHLDVATKGDELGRTTYVDSNLDPLRVERGGVFVGQSVNIPFFWRHVLAALDLADAQVLSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBW1</accession>
    <name>A0A1A0HBW1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_42053</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711980.1">
        <property type="nucleotide sequence ID" value="XM_018857490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBW1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBW1"/>
    <dbReference type="GeneID" id="30030466"/>
    <dbReference type="OrthoDB" id="4082764at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024297">
        <property type="entry name" value="Pho86"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11124">
        <property type="entry name" value="Pho86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="118"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="335" mass="37884" checksum="01DA77577EFD8B75" modified="2016-10-05" version="1">MAPQKTVDLNKPLDAAEEAVIRRVPLEPELATVALTLHGDIYRQAQGKTNHAIVWHPLTQAYICIVFLVSTAYVFKDLVEVLDSMREFFDLLMHNKYVLTRYFPAMIFVAGTVGLISFTITDEFRVISDKMASDKYMAQVFRFPLRIYANAQPTEQNSAFVKSASQSTDFIEYRNSPIAVVTVVPLPNQSTSDTFFAKITGLHVRKSYRSAGAESDLLEYAKEKARLLCSRYVADNKIHTKNMRIVLVAEAYSVDQNLTSLYQKLGFSVKNKTTKLDPYSAEATKHFFYVIPAGALLNFFGVFRVTYELQLDNVVDLVAESSAAKKNKNKLRKRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC85</accession>
    <name>A0A1A0HC85_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial outer membrane protein porin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_41376</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic mitochondrial porin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712018.1">
        <property type="nucleotide sequence ID" value="XM_018857390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC85"/>
    <dbReference type="STRING" id="869754.A0A1A0HC85"/>
    <dbReference type="GeneID" id="30030366"/>
    <dbReference type="OrthoDB" id="7827681at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07306">
        <property type="entry name" value="Porin3_VDAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.10:FF:000012">
        <property type="entry name" value="Voltage-dependent anion-selective channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001925">
        <property type="entry name" value="Porin_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743:SF70">
        <property type="entry name" value="GH26960P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11743">
        <property type="entry name" value="VOLTAGE-DEPENDENT ANION-SELECTIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00185">
        <property type="entry name" value="EUKARYTPORIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00558">
        <property type="entry name" value="EUKARYOTIC_PORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0626">Porin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="282" mass="29671" checksum="338556ED6FE687DA" modified="2016-10-05" version="1">MAPVAFSDLQKASNDLLNKDFFHLSTAAVDVKTVAPNGVAFTVKGKTAKDNAIAASVEAKYADKATGLTLTEGWNNSNTLNTKIELSELLTPGLKGELDTSVVPNGAKNAKLNFFYQQSAVNARLFFDLLKGPVATADLAVAHDGFTAGAELGYDIASAKLNKYSVGVGYANPLYTVGVTSTSNLSVFTAAYFHKVSPFVEAGAKATWDSVKSQNVNVEFATKYKLDSSAFVKAKIADSGLTALAYSQELRPGVRLGLGASFDALKLAEPVHKLGVSLSFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCH5</accession>
    <name>A0A1A0HCH5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_78524</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712123.1">
        <property type="nucleotide sequence ID" value="XM_018858893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCH5"/>
    <dbReference type="STRING" id="869754.A0A1A0HCH5"/>
    <dbReference type="GeneID" id="30031869"/>
    <dbReference type="OrthoDB" id="1677536at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000002">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="123"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="132" mass="14099" checksum="025599232B1BF35F" modified="2016-10-05" version="1">MSTDRSLVFGVARIFASFNDTFVHVTDLSGRETICRVTGGMKVKADRDESSPYAAMLAAQDVAEKCKEVGITAVHVKLRATGGTKTKTPGPGGQAALRALARSGLRIGRIEDVTPVPSDSTRRKGGRRGRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCW2</accession>
    <name>A0A1A0HCW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30855</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712347.1">
        <property type="nucleotide sequence ID" value="XM_018855727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCW2"/>
    <dbReference type="GeneID" id="30028703"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="102" mass="11459" checksum="16E0A379478B609D" modified="2016-10-05" version="1">MSYILVSSTWVILRFILPGSYYGLISSLGSASKIHLRSVNKNTVRPPNELASVCPTPAFKALHVRTHIMSDPPVSYAHLIDVYTVWNVWSFSFFLHLSDPPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDA7</accession>
    <name>A0A1A0HDA7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RNA polymerase II assembly factor Rtp1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_31030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tango6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712560.1">
        <property type="nucleotide sequence ID" value="XM_018855757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDA7"/>
    <dbReference type="GeneID" id="30028733"/>
    <dbReference type="OrthoDB" id="39591at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057407">
        <property type="entry name" value="HEAT_TANGO6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019451">
        <property type="entry name" value="Rtp1_C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019414">
        <property type="entry name" value="Rtp1_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039600">
        <property type="entry name" value="TANGO6/Rtp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20959:SF1">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23565">
        <property type="entry name" value="ARM_TANGO6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10363">
        <property type="entry name" value="RTP1_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10304">
        <property type="entry name" value="RTP1_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TANGO6 HEAT repeat" evidence="5">
        <location>
            <begin position="299"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="4">
        <location>
            <begin position="716"/>
            <end position="816"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase II assembly factor Rtp1 C-terminal" evidence="3">
        <location>
            <begin position="1070"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="614"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="801"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="614"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="625"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="832"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="846"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="883"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="894"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1106" mass="119240" checksum="5039B6056CF26D51" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDR3</accession>
    <name>A0A1A0HDR3_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">HSP90-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_39059</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712559.1">
        <property type="nucleotide sequence ID" value="XM_018857088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDR3"/>
    <dbReference type="STRING" id="869754.A0A1A0HDR3"/>
    <dbReference type="GeneID" id="30030064"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140453">
        <property type="term" value="C:protein aggregate center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.790:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.80:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11260:FF:000001">
        <property type="entry name" value="Heat shock protein 90 alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000001">
        <property type="entry name" value="Heat shock protein HSP 90-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="10">
        <location>
            <begin position="28"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="216"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="677"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="515"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="121"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="700" mass="80045" checksum="EDD42B56EC8483F4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW5</accession>
    <name>A0A1A0HDW5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNI-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712697.1">
        <property type="nucleotide sequence ID" value="XM_018857184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW5"/>
    <dbReference type="STRING" id="869754.A0A1A0HDW5"/>
    <dbReference type="GeneID" id="30030160"/>
    <dbReference type="OrthoDB" id="8436363at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1131"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1144"/>
            <end position="1154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1184" mass="132286" checksum="FBD5EC7A68DD420A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEM7</accession>
    <name>A0A1A0HEM7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">catechol O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_11261</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.6" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712921.1">
        <property type="nucleotide sequence ID" value="XM_018853945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEM7"/>
    <dbReference type="STRING" id="869754.A0A1A0HEM7"/>
    <dbReference type="GeneID" id="30026921"/>
    <dbReference type="OrthoDB" id="186626at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836:SF2">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13578">
        <property type="entry name" value="Methyltransf_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="254" mass="28652" checksum="AAB9CFABB1D35E2F" modified="2016-10-05" version="1">MEQQVLQHVLSTPGTKGSPEKVLQAIDEFAQKRRLMIVGRQKGLLVLDAIREKEPAIMVELGCYVGYSAILFGAELATQNEKYSENYNTPARYYSFEICPEFAAVARQMVEHAGLSGTVEIIVGPAGRTLPQFQHRLKEQFHKYTPVDVVFVDHAKELYVPDFRVLESQGLIAPDTVIVADNIFLPGAPDYVQYVQSSPEYRRDHNQRTINVSNAECEGRWNLLYDSRTVPVEDPETGRRDAVEITRCVSYLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HET8</accession>
    <name>A0A1A0HET8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_153317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712919.1">
        <property type="nucleotide sequence ID" value="XM_018854586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HET8"/>
    <dbReference type="GeneID" id="30027562"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="22907" checksum="2BD0F71CDFBB4DAF" modified="2016-10-05" version="1">MHIHSLFKNISLLSIKPPSYKLDPIFTASSSICSFKLKRSAFDNLGQGCIPVFVLWFPNQGNGRLGGQSSNKRIGATSTLYREFRCTASGYSLTSPIWKMTEIFSDYESNSTVCSSHARPSRHSLGQVRAKPLVPPHFLVFVSQPQRHTYRQTFPSRSVVPGTLINPNHNAILVARTTRCAQNSGPNRKGLPGPHTNRPFANVFHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEW5</accession>
    <name>A0A1A0HEW5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_31428</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713037.1">
        <property type="nucleotide sequence ID" value="XM_018855848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEW5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEW5"/>
    <dbReference type="GeneID" id="30028824"/>
    <dbReference type="OrthoDB" id="1938591at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12191">
        <property type="entry name" value="gal11_coact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033789">
        <property type="entry name" value="Gal11_coact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008626">
        <property type="entry name" value="Mediator_Med15_fun"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18535">
        <property type="entry name" value="Gal11_ABD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
        <property type="entry name" value="KIX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05397">
        <property type="entry name" value="Med15_fungi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mediator complex subunit 15 KIX" evidence="6">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Gal11 coactivator" evidence="7">
        <location>
            <begin position="167"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="444"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="757"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="998"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="452"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="525"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="760"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="770"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="818"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="830"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="878"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="895"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="910"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1014"/>
            <end position="1024"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA22541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1121" mass="123139" checksum="0FAE7A1AF8E6F28B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEZ8</accession>
    <name>A0A1A0HEZ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ARM repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_39939</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713072.1">
        <property type="nucleotide sequence ID" value="XM_018857208.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HEZ8"/>
    <dbReference type="GeneID" id="30030184"/>
    <dbReference type="OrthoDB" id="5148094at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043008">
        <property type="term" value="F:ATP-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170011">
        <property type="term" value="F:stalled ribosome sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034198">
        <property type="term" value="P:cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140469">
        <property type="term" value="P:GCN2-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071264">
        <property type="term" value="P:positive regulation of translational initiation in response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990611">
        <property type="term" value="P:regulation of cytoplasmic translational initiation in response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006448">
        <property type="term" value="P:regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057546">
        <property type="entry name" value="HEAT_GCN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346:SF7">
        <property type="entry name" value="STALLED RIBOSOME SENSOR GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23346">
        <property type="entry name" value="TRANSLATIONAL ACTIVATOR GCN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24984">
        <property type="entry name" value="HEAT_EF3_GNC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24987">
        <property type="entry name" value="HEAT_EF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23271">
        <property type="entry name" value="HEAT_GCN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25801">
        <property type="entry name" value="HEAT_GCN1_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="3">
        <location>
            <begin position="232"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="415"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="527"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="3">
        <location>
            <begin position="559"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="2">
        <location>
            <begin position="873"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="3">
        <location>
            <begin position="897"/>
            <end position="1128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1588" mass="174215" checksum="526AB234E8DFD324" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF34</accession>
    <name>A0A1A0HF34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ZIP zinc/iron transport family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_11324</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712994.1">
        <property type="nucleotide sequence ID" value="XM_018853958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF34"/>
    <dbReference type="STRING" id="869754.A0A1A0HF34"/>
    <dbReference type="GeneID" id="30026934"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000007">
        <property type="term" value="F:low-affinity zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071578">
        <property type="term" value="P:zinc ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF69">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="366" mass="40328" checksum="CC7CE1106247E6E9" modified="2016-10-05" version="1">MFFDEIAKRESCGLISSNGEHAELMGLRISSVFVVLVGSGFGVFFPILASRYSFVRMPSWVFFFCKYFGSGVIISTAFIHLLFEANENLTSECLSNAWQNYPYAFGIMLGTLFALFLAEIMSFRFVESKLAGLDNHGHSHFGDESVYVERPGEFDDEISLVTLESADPGLLAEKYPPRIIDVPDLDEKGTLDTPNDNQDKEQYFGQLVSIFVLEFGVLFHSVFVGLTLGITSTQFTTLYIVIIFHQLFEGLGLGSRIAATIWPKGKHWIPWVFAAGFTLVTPIGIAIGLGVSGTYPSDSPRALITNGVFDSISAGILIYSGLVELMAHEFLFSTEFKGAGGTKRMLWAYGCMCLGCGLMSLLGRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF58</accession>
    <name>A0A1A0HF58_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="19">CFEM-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_31506</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBT5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713132.1">
        <property type="nucleotide sequence ID" value="XM_018855868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF58"/>
    <dbReference type="STRING" id="869754.A0A1A0HF58"/>
    <dbReference type="GeneID" id="30028844"/>
    <dbReference type="OrthoDB" id="2496787at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051735">
        <property type="entry name" value="CFEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6 15" id="KW-0349">Heme</keyword>
    <keyword evidence="10 15" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291779" evidence="17">
        <location>
            <begin position="18"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="18">
        <location>
            <begin position="17"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="138"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="138"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="49"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="59"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="15">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA22636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="272" mass="27226" checksum="5C2987AB7279BFDC" modified="2016-10-05" version="1">MLSAFIPLASLLALAQAGYETYPTVAKTASINGFADKIYDRLPVCAQACVSESTSSTPCPYWDSGCLCVMSNFASNIADCFAGNCVGDDVQSARSLATSICSSAGVQSPHWFIDSSASSSLAAAALKVASVEPTAAETTAAETTAAESPAAVSTANTEPVASTLETENTASTSAAVTRAISSTETESAHTESAHTESAHTESAHTESAHTESAHTEASAYVSSMTSASESTMSSFSSATSWSTPAVSSAFNAAGSAVPAAACGLAAGVALIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFI2</accession>
    <name>A0A1A0HFI2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA repair protein REV1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Reversionless protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_37651</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Involved in mitochondrial DNA mutagenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713226.1">
        <property type="nucleotide sequence ID" value="XM_018856902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFI2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFI2"/>
    <dbReference type="GeneID" id="30029878"/>
    <dbReference type="OrthoDB" id="427711at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017125">
        <property type="term" value="F:deoxycytidyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01701">
        <property type="entry name" value="PolY_Rev1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000001">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10190:FF:000011">
        <property type="entry name" value="DNA repair protein REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031991">
        <property type="entry name" value="Rev1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16727">
        <property type="entry name" value="REV1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BRCT" evidence="18">
        <location>
            <begin position="151"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="UmuC" evidence="19">
        <location>
            <begin position="434"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="908"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="985"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="908"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA22745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1132" mass="128083" checksum="B67A4B7E4459CDF5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFK1</accession>
    <name>A0A1A0HFK1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_29493</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713410.1">
        <property type="nucleotide sequence ID" value="XM_018855415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFK1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFK1"/>
    <dbReference type="GeneID" id="30028391"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10145">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.190:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIA subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009415">
        <property type="entry name" value="Hum_TFIIA_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="10">
        <location>
            <begin position="66"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR009415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="121" mass="13701" checksum="B6F1D04D6A5F9627" modified="2016-10-05" version="1">MSSPAYYELYRRSAIGSALADALDTLISDEKIQPQLAMRILNNFDRIISESLKAERGLCKSKITFKGDLHTYRFCDDVWTFIIKNVNIKMTDVSNNDLNDSDLTAEKFKIVACNSRKAGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFM1</accession>
    <name>A0A1A0HFM1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_29252</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713139.1">
        <property type="nucleotide sequence ID" value="XM_018855371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFM1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFM1"/>
    <dbReference type="GeneID" id="30028347"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="151"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="315" mass="35751" checksum="7259137B23A23B4A" modified="2016-10-05" version="1">MSLITVKSRSPSIKGFSEDLVSFSVSRIYEKVAANSKLPVSQVKLSVLGADGKHKPVDSDSTLTEHFDAQSLLGEVVLYAKDLGRQIAWQTVFLLEYLGPILIHLVVYFVAAVLFGVAQSQTQKLALVLAVLHFVKREYETLFVHRFSNSTMPLFNLFKNSGHYWILSGVNLSVFVYSYSPTSLEAAGPLASFLFHVNTWPQLLNYALVALWVFAEVSNFITHVKLAGIRSTDKKAYAIPYGYGFDWVVCPNYFFESLSWLLYALLVGNWSAWLFLVISSTQMYMWALKKNRRYLSTFGDEYKKLKRAKFIPFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFW3</accession>
    <name>A0A1A0HFW3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_28794</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713268.1">
        <property type="nucleotide sequence ID" value="XM_018855361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFW3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFW3"/>
    <dbReference type="GeneID" id="30028337"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11387">
        <property type="entry name" value="bHLHzip_USF_MITF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF3">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="57"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="12"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="35"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="211" mass="23088" checksum="D246BCD5469F3685" modified="2016-10-05" version="1" fragment="single">ILARSLDIKKQLQQSFHGHSSPMSGSGGVTKPRARRDSAYHIKTEDGDAETRNENDRNEAEEQGIERKRRDNINDKIQELLELIPGAYFQDTSVDAGMAPGHPFDEALALAKSTGTRDGKPNKGQILTQAVEYIQFLQNVIDENNRKEVELCLRMQSLHLLRSGGLNPPSDVPMAVGPTSAEQALGEIGVGPESDAYFRAVLLESHGGIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFX5</accession>
    <name>A0A1A0HFX5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_29462</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713366.1">
        <property type="nucleotide sequence ID" value="XM_018855407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFX5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFX5"/>
    <dbReference type="GeneID" id="30028383"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140569">
        <property type="term" value="P:extraction of mislocalized protein from ER membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055092">
        <property type="term" value="P:sterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000049">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01229">
        <property type="entry name" value="COF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="997"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1025"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1062"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1142"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1181"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="14">
        <location>
            <begin position="21"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="13">
        <location>
            <begin position="255"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1215" mass="135404" checksum="C6EF4D52AEE741FD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG23</accession>
    <name>A0A1A0HG23_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_85314</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bud neck</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713432.1">
        <property type="nucleotide sequence ID" value="XM_018859246.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HG23"/>
    <dbReference type="GeneID" id="30032221"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005940">
        <property type="term" value="C:septin ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060258">
        <property type="term" value="P:negative regulation of filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12194">
        <property type="entry name" value="Kcc4p_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14081">
        <property type="entry name" value="STKc_BRSK1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000394">
        <property type="entry name" value="Serine/threonine-protein kinase HSL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.220">
        <property type="entry name" value="Fungal kinase associated-1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031850">
        <property type="entry name" value="Fungal_KA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043024">
        <property type="entry name" value="KA1_sf_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16797">
        <property type="entry name" value="Fungal_KA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="25"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="367"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="672"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="786"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="987"/>
            <end position="1133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="611"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="367"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="404"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="425"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="694"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="720"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1021"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1096"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1294" mass="145375" checksum="76E1614552BE431F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG79</accession>
    <name>A0A1A0HG79_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="2">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">E2 ubiquitin-conjugating enzyme 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Ubiquitin carrier protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ubiquitin-protein ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_37377</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713476.1">
        <property type="nucleotide sequence ID" value="XM_018856867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG79"/>
    <dbReference type="STRING" id="869754.A0A1A0HG79"/>
    <dbReference type="GeneID" id="30029843"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990389">
        <property type="term" value="C:CUE1-UBC7 ubiquitin-conjugating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000837">
        <property type="term" value="C:Doa10p ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000839">
        <property type="term" value="C:Hrd1p ubiquitin ligase ERAD-L complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180027">
        <property type="term" value="P:inner nuclear membrane-associated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23796">
        <property type="entry name" value="UBCc_UBE2G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000008">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0882">Thioester bond</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="6"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA22995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="167" mass="18722" checksum="4858539BE7FDD3DB" modified="2016-10-05" version="1">MAPRTTAQRRLLKEYQQLALDPPPGIVAGPISENDLFVWDCLLEGPAETPYEHGVFSAILKFPKDYPLAPPVLKFDPPLLHPNIYADGTVCISILHPPGEDPNQYERPEERWSPIQSIEKILLSVVSMLAEPNPESGANIDACKLWRDDRASFDAQVREQVKRNLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG83</accession>
    <name>A0A1A0HG83_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inorganic phosphate transport PHO88</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713481.1">
        <property type="nucleotide sequence ID" value="XM_018856467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG83"/>
    <dbReference type="STRING" id="869754.A0A1A0HG83"/>
    <dbReference type="GeneID" id="30029443"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="191" mass="21094" checksum="519938F9B821BA1F" modified="2016-10-05" version="1">MNPAFSNLGLMLIMMQVSRRLDLEDPQILMYVRIAYVACQLVVFGVCMYVKFQINRKNDLTTMKYVEPGNPMAGQEEKAVVTTVKEYDLKQIDSQIKSIFTSLAMMGFMHLYMKYTNPLVMQSVSSVKSALETNIVKIHLWGNPASGDLKRPFKAAPGLLLAFQGASGDIKTDKASVEAAETAGAGGIKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGG6</accession>
    <name>A0A1A0HGG6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29526</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713453.1">
        <property type="nucleotide sequence ID" value="XM_018855426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGG6"/>
    <dbReference type="GeneID" id="30028402"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="9090" checksum="7F39C457115713EF" modified="2016-10-05" version="1" fragment="single">MKFCESTLGTLFIHTLELISFHGLRSIFDAFFQAPLMKDNTPKKILNQLPIFLLFGKLAMKGFHFVYPPFVCYADITSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGJ1</accession>
    <name>A0A1A0HGJ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_96132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713478.1">
        <property type="nucleotide sequence ID" value="XM_018859422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGJ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGJ1"/>
    <dbReference type="GeneID" id="30032397"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA22997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="218" mass="24548" checksum="8A02990E7DB1FB80" modified="2016-10-05" version="1">MFFGGSLARDGTKPFIIDFKNEWHNTGMIVFRRNYGKVPLYINGFTDGTPVIKNSGSICLYSTTWDVQTTIEGNGCISVGYDSILHVELRQNRVRAPSGQVIYLESFNSYLRISGLRLELHYEPEFKVAGFGQNNVIEFDIDINHISLLNYHADSGTIFVASDRYLDVSFVIGKGYDGFAIKVTLSPNGSKLTYDRPVPDVSRPSECRCQSHFPSVFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGP4</accession>
    <name>A0A1A0HGP4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_9465</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713646.1">
        <property type="nucleotide sequence ID" value="XM_018859399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGP4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGP4"/>
    <dbReference type="GeneID" id="30032374"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="323" mass="35143" checksum="EC754BF031E12FF5" modified="2016-10-05" version="1">MPDWRLPCWGLLRWLLALFFFPITTTVPTTIPISILIPSHITVTITITAPITSPINVTIRSPTTTVPITIHLTVPITINITVPNTITIIVHITTTAPITITITITITSPIPSPILVSLTISITVPITITITVPITSPISVPITVPITITTIVPIAIIVPITITIIVPITTTVPIPVTITIPYCSYYHYHYHYYYYPITTTVSITIPLPVPLPVPVANTLPFPSTIPAGPLQSRPRCAASQSLIWGRLWPAARAGPGEPRQARPLRSRSTPKTHGLYSKRFQKLAASVGPYRALFLTLCMPGTFGQQASPGFPRPGRAFFRAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGU0</accession>
    <name>A0A1A0HGU0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_34040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713549.1">
        <property type="nucleotide sequence ID" value="XM_018856433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGU0"/>
    <dbReference type="STRING" id="869754.A0A1A0HGU0"/>
    <dbReference type="GeneID" id="30029409"/>
    <dbReference type="OrthoDB" id="5325112at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034058">
        <property type="term" value="P:endosomal vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032914">
        <property type="entry name" value="Vam6/VPS39/TRAP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894">
        <property type="entry name" value="CNH DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12894:SF27">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR-ASSOCIATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="CNH" evidence="6">
        <location>
            <begin position="133"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="1035"/>
            <end position="1062"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123792" checksum="5B9337FC67B8E04A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGW8</accession>
    <name>A0A1A0HGW8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Chromatin modification-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_9529</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17">Component of an histone acetyltransferase complex.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in cell cycle progression and meiosis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of an histone acetyltransferase complex. Interacts with H3K4me3 and to a lesser extent with H3K4me2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="17">The PHD-type zinc finger mediates the binding to H3K4me3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the ING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713722.1">
        <property type="nucleotide sequence ID" value="XM_018859404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGW8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGW8"/>
    <dbReference type="GeneID" id="30032379"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032777">
        <property type="term" value="C:piccolo histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140002">
        <property type="term" value="F:histone H3K4me3 reader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16858">
        <property type="entry name" value="ING_ING3_Yng2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000436">
        <property type="entry name" value="Chromatin modification-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024610">
        <property type="entry name" value="ING_N_histone-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF100">
        <property type="entry name" value="CHROMATIN MODIFICATION-RELATED PROTEIN YNG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12998">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01408">
        <property type="entry name" value="ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7 17" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 15 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15 17" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 16 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="19">
        <location>
            <begin position="202"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="130"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="143"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="159"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="14">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="14">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="14">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="14">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00063794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA23241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="262" mass="30033" checksum="F29541F607A30342" modified="2016-10-05" version="1">MDTTAVLDKYTQDLLNLPLEVKHLFEELKAKDTQLAEARRRYQTKDSQLHKFIRANGTLTRHPKEQQICSKIDEDMKLVQKIQKEKILLANTALFLVSKHMCNFEADIQKLERDELIAPLDESAQIEEIDGALETPRSSPAPVKKKRTLSVMKQRTKRPKSEDAEDSGHGEHTHSGHSRAHTVNAGSLAMKTSNGAEDADNNLYCFCQRVSFGEMIGCDNDDCQYEWFHWECVGITSPPNDDEIWYCPDCAPKMEKRKKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGZ7</accession>
    <name>A0A1A0HGZ7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Suf-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9429</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PRP39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713604.1">
        <property type="nucleotide sequence ID" value="XM_018859386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGZ7"/>
    <dbReference type="STRING" id="869754.A0A1A0HGZ7"/>
    <dbReference type="GeneID" id="30032361"/>
    <dbReference type="OrthoDB" id="10265668at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030627">
        <property type="term" value="F:pre-mRNA 5'-splice site binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000395">
        <property type="term" value="P:mRNA 5'-splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204">
        <property type="entry name" value="PRE-MRNA PROCESSING PROTEIN PRP39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17204:SF5">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="461"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="461"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="482"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="756" mass="83016" checksum="6D601229EB9FB99B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH37</accession>
    <name>A0A1A0HH37_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_103995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713791.1">
        <property type="nucleotide sequence ID" value="XM_018853757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH37"/>
    <dbReference type="GeneID" id="30026733"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="233" mass="26112" checksum="A4CF8B397ADE0957" modified="2016-10-05" version="1">MPCLSKLNAMFIEIKGMPIEIKGHAYRNQRPCFSKSKAMFIEIKGHTDHTKTQRNGMHHRGPGHGHIFVQFDWLREVERFFRQLCHTWRRGGDDIDGPYNRLVRKKTKKKNHLPRTSTSAPEKHIGMFRATQPGHAEGVRPASSGRVSTAVSPQAPVFGCSLSPPSRVFLGVSCTGVGARSRARWVQGGRGGAINHTTWRVFCAGSRKRPCPGARAWPPWAACKNGRLQKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH54</accession>
    <name>A0A1A0HH54_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Myb-like transcription factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37982</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713654.1">
        <property type="nucleotide sequence ID" value="XM_018856938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH54"/>
    <dbReference type="STRING" id="869754.A0A1A0HH54"/>
    <dbReference type="GeneID" id="30029914"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
        <property type="term" value="C:snRNA-activating protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051575">
        <property type="entry name" value="Myb-like_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="28"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="93"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="93"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="19101" checksum="84A22C43E1D88115" modified="2016-10-05" version="1" fragment="single">MSCKDKPKKSIYSVDTLAFTESLGLQTFRKEQRKTWMKDDDDHLLRRLADLYPDEYKSNSLKAGAINWELVSQAFGGERKAKDCRKRWALSLDPNLRRGKWTPEEDALLVLQFEKYGALWQQVAQDIAGRTEHQCLKRYYEVLDPSVSNRLKLWTEAEDLMLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH68</accession>
    <name>A0A1A0HH68_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29973</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714005.1">
        <property type="nucleotide sequence ID" value="XM_018855527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH68"/>
    <dbReference type="STRING" id="869754.A0A1A0HH68"/>
    <dbReference type="GeneID" id="30028503"/>
    <dbReference type="OrthoDB" id="550424at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.230.10:FF:000001">
        <property type="entry name" value="DnaJ subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.230.10">
        <property type="entry name" value="Heat shock protein DnaJ, cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.260.20">
        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044713">
        <property type="entry name" value="DNJA1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43888">
        <property type="entry name" value="DNAJ-LIKE-2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00684">
        <property type="entry name" value="DnaJ_CXXCXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="DnaJ/Hsp40 cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="HSP40/DnaJ peptide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291801" evidence="7">
        <location>
            <begin position="20"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="22"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="9">
        <location>
            <begin position="141"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="6">
        <location>
            <begin position="141"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="359" mass="39509" checksum="3B503117451BEBB9" modified="2016-10-05" version="1">MIAVRLISMACLLACLVAAGLDPYEVLGVPRDADDRTIKSQYRQLSKQYHPDKNPDPEAHERFLQVGEAYGILDDPQKRENYDKYGDPDGGAGQQGGFNDIFNQFFHGGHAGFGGQRQQRQQRGGDTQVVVRLSLQDFYVGKDLEFSVHMLNICAACSGSGSADSKRKTCGKCSGSGMFTQTVQFGPMIQRVQTPCDQCQGSGSTIANKCQACGGARVQKAPRNYSVFVAPGTPKGHTHVLEGEGDQSPDWVPGNLNIRFVEAEHENWGFRRVGDHLYRTEVLTAQEAGAGGWQREIRMFDDEVVTLRRDKGHAVMDGQVDIIKGHGMPHAHDHDEYGHLYVDYKVLPVGAAALEKDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH72</accession>
    <name>A0A1A0HH72_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_35512</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713708.1">
        <property type="nucleotide sequence ID" value="XM_018856619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH72"/>
    <dbReference type="STRING" id="869754.A0A1A0HH72"/>
    <dbReference type="GeneID" id="30029595"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15737">
        <property type="entry name" value="FYVE2_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052727">
        <property type="entry name" value="Rab4/Rab5_effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510">
        <property type="entry name" value="FYVE-FINGER-CONTAINING RAB5 EFFECTOR PROTEIN RABENOSYN-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510:SF44">
        <property type="entry name" value="RABENOSYN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="42"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="311"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="601"/>
            <end position="628"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="71586" checksum="AE8B72A24D2353AD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHJ0</accession>
    <name>A0A1A0HHJ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_30066</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Cytoplasmic dynein acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required to maintain uniform nuclear distribution in hyphae. May play an important role in the proper orientation of the mitotic spindle into the budding daughter cell yeast. Probably required for normal progression of the cell cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Consists of at least two heavy chains and a number of intermediate and light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714110.1">
        <property type="nucleotide sequence ID" value="XM_018855547.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HHJ0"/>
    <dbReference type="GeneID" id="30028523"/>
    <dbReference type="OrthoDB" id="447173at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000741">
        <property type="term" value="P:karyogamy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902850">
        <property type="term" value="P:microtubule cytoskeleton organization involved in mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein 1 heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000996">
        <property type="entry name" value="Cytoplasmic dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.180.20:FF:000002">
        <property type="entry name" value="Cytoplasmic dynein heavy chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002357">
        <property type="entry name" value="Glutathione S-transferase class-mu 26 kDa isozyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.180.20">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.720">
        <property type="entry name" value="Region D6 of dynein motor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035706">
        <property type="entry name" value="AAA_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041658">
        <property type="entry name" value="AAA_lid_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042219">
        <property type="entry name" value="AAA_lid_11_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054354">
        <property type="entry name" value="DYNC2H1-like_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041466">
        <property type="entry name" value="Dynein_AAA5_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024317">
        <property type="entry name" value="Dynein_heavy_chain_D4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004273">
        <property type="entry name" value="Dynein_heavy_D6_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013594">
        <property type="entry name" value="Dynein_heavy_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042228">
        <property type="entry name" value="Dynein_linker_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703">
        <property type="entry name" value="DYNEIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45703:SF36">
        <property type="entry name" value="DYNEIN HEAVY CHAIN, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12775">
        <property type="entry name" value="AAA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12780">
        <property type="entry name" value="AAA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12781">
        <property type="entry name" value="AAA_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18198">
        <property type="entry name" value="AAA_lid_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08385">
        <property type="entry name" value="DHC_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22597">
        <property type="entry name" value="DYN_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17852">
        <property type="entry name" value="Dynein_AAA_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03028">
        <property type="entry name" value="Dynein_heavy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5" id="KW-0415">Karyogamy</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-0505">Motor protein</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="1847"/>
            <end position="2011"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="2131"/>
            <end position="2289"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="2484"/>
            <end position="2639"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="3315"/>
            <end position="3370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00053342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA23629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="4188" mass="477358" checksum="BFB43AAB541F5926" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI02</accession>
    <name>A0A1A0HI02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutamyl-tRNA(Gln) amidotransferase subunit F, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_38133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714112.1">
        <property type="nucleotide sequence ID" value="XM_018856958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI02"/>
    <dbReference type="STRING" id="869754.A0A1A0HI02"/>
    <dbReference type="GeneID" id="30029934"/>
    <dbReference type="OrthoDB" id="4024285at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20977">
        <property type="entry name" value="GatF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="16985" checksum="E58576B5F8CF219C" modified="2016-10-05" version="1">MLRRSLFTSAGALQGKVYPKLQNVSELVGFLNKSNENVHDLVPGPTSVVDSKIVRKMLRLSGLESDISAETEQKWIQALNTQIGFINHLQPSGSHADTSENMNGAIFRLISSDHQPSEALGLEQLMEQVRANQLQSSGEKIKDFDQFVSSSVDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI74</accession>
    <name>A0A1A0HI74_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tafazzin family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_30245</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol + a 1,2-diacyl-sn-glycero-3-phosphocholine = a cardiolipin + a 1-acyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:33731"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64743"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:33732"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:33733"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion outer membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the taffazin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714350.1">
        <property type="nucleotide sequence ID" value="XM_018855583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI74"/>
    <dbReference type="STRING" id="869754.A0A1A0HI74"/>
    <dbReference type="GeneID" id="30028559"/>
    <dbReference type="OrthoDB" id="193467at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035965">
        <property type="term" value="P:cardiolipin acyl-chain remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007007">
        <property type="term" value="P:inner mitochondrial membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097250">
        <property type="term" value="P:mitochondrial respirasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000872">
        <property type="entry name" value="Tafazzin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497:SF0">
        <property type="entry name" value="TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12497">
        <property type="entry name" value="TAZ PROTEIN TAFAZZIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00979">
        <property type="entry name" value="TAFAZZIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="13">
        <location>
            <begin position="71"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="410" mass="46957" checksum="32C605EA5AA83912" modified="2016-10-05" version="1">MSYPDVLKRGEDFLSEYPRTSPWWNYVSHATCFGMVLGSKFFLNLFYNPQVHNIEKLDWALEKARKENRALLTVMNHMSVVDDPGFYSMLPMRFHTDIDTVRWGFGAHNICFSTPSMSWFFNLGKILGTKRFGEGPFQGSVDAAIRILSPDDTLDLEFSPWVKEREKPSLVQEINNIGGLVSEKVGQAVQAVKPNAEATNILMSKSPFVRSKTSWFHVFPEGFVLQLEEPENNSMRYFKWGVSRLILESTRTPVVVPIFSHGFEKIVPEDSPSDGFDRWIPKNIGAEIHVTIGDAIKDELLESYRNEWRKLCAKYTDPKNPQDLSPELMNGKKAQTLRSKLAADLRRHVLAIREGLGKFAPENQKFKDPAFWKAYTLSEGASDKDVKFIGQNWAIKRLQKHLPEYEDGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HI83</accession>
    <name>A0A1A0HI83_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_110948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714196.1">
        <property type="nucleotide sequence ID" value="XM_018853918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HI83"/>
    <dbReference type="GeneID" id="30026894"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="16660" checksum="A6E504536A480A55" modified="2016-10-05" version="1">MVLSVTCDLLFPCSARYSERPNTPLKADLTFHHFGFSQRILDTSDTLLPKSRLQRIPFCRNEEKPCLAGSFVFTFHNGRPVISFRHCCAPRELMIEAAAGNKALTDFKTTDRNCSTSSLCALSKRIPWSPAGAAAKWRAQRGTGLPQTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HID7</accession>
    <name>A0A1A0HID7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DAGKc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_34954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714084.1">
        <property type="nucleotide sequence ID" value="XM_018856544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HID7"/>
    <dbReference type="GeneID" id="30029520"/>
    <dbReference type="OrthoDB" id="3853857at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001727">
        <property type="term" value="F:lipid kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050187">
        <property type="entry name" value="Lipid_Phosphate_FormReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358:SF108">
        <property type="entry name" value="DAGKC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12358">
        <property type="entry name" value="SPHINGOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAGKc" evidence="1">
        <location>
            <begin position="43"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="350" mass="39519" checksum="DD7DCD114FC280BA" modified="2016-10-05" version="1">MLLLNQNDVYVLDSTKSGSQLRTKEAGFYGRILQPLFDDLFLVKHKYVATTSSVSIHEFSSTLQSNGRPILMIIIAGDTSISEFVNSLSKVDMGQIKIFVVPAGTGNSLALSLGIIDERIAVQRLFQYDEESTRPLNLYQAKFPLGSYFFKNTDRQQLLPNIVLFVVVASWAFHASLVADSDTEEMRKHGTSRFKVAAHENLKQLQEYRGDVVISDDTGSRITHKGPFAYLVVTPSQKFEPSFQILPKGDIFNSDLYLVGFQTQNNGNYILDIMKEVYNDGNHITDQRVFYDKVDKHLRIRLTLGPNETISRRRFCIDGAIVIVPNNEGDYVTFKNHGSHVNGWNILIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIQ1</accession>
    <name>A0A1A0HIQ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_30384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714515.1">
        <property type="nucleotide sequence ID" value="XM_018855628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIQ1"/>
    <dbReference type="GeneID" id="30028604"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="51" mass="6371" checksum="101826184B9653AB" modified="2016-10-05" version="1">MERTIRSFVQSLDIRFSMLYLHRTISQQQRRHRRFPKPYLQKLRNIQILVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIU1</accession>
    <name>A0A1A0HIU1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_114928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714402.1">
        <property type="nucleotide sequence ID" value="XM_018854004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIU1"/>
    <dbReference type="GeneID" id="30026980"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="87" mass="10183" checksum="80C39DD91D81C8DB" modified="2016-10-05" version="1">MNLPKKNNNEYEIVHFWCQKFGFQTSMHLGEMLAPLGTFFDNLKILNWFDKGVRRVYLLRDAKTNVPFAALSNDLWSQAPEKVYPMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJE9</accession>
    <name>A0A1A0HJE9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Dihydropteroate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_37497</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate = 7,8-dihydropteroate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19949"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="EC" id="2.5.1.15"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the C-terminal section; belongs to the DHPS family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the DHNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714493.1">
        <property type="nucleotide sequence ID" value="XM_018856880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJE9"/>
    <dbReference type="STRING" id="869754.A0A1A0HJE9"/>
    <dbReference type="GeneID" id="30029856"/>
    <dbReference type="OrthoDB" id="615426at2759"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="16">
        <location>
            <begin position="459"/>
            <end position="758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00009951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA24012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="768" mass="84206" checksum="7E0E79310BEDB027" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJF8</accession>
    <name>A0A1A0HJF8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CHCH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_35349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714775.1">
        <property type="nucleotide sequence ID" value="XM_018856601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJF8"/>
    <dbReference type="STRING" id="869754.A0A1A0HJF8"/>
    <dbReference type="GeneID" id="30029577"/>
    <dbReference type="OrthoDB" id="5586401at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010625">
        <property type="entry name" value="CHCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039870">
        <property type="entry name" value="Coa4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13639:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY FACTOR 4 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06747">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHCH" evidence="2">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA24294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="74" mass="8670" checksum="2F9A2F013ACCC094" modified="2016-10-05" version="1">MKPATNITLQEDDDTPDEWDQRILKTGCYEENLALQLCHADEGDWRKCLGQMAAFRKCWEANKNNERTHTVDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJH4</accession>
    <name>A0A1A0HJH4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ras modification protein ERF4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_10185</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Interacts with ERF2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERF4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714469.1">
        <property type="nucleotide sequence ID" value="XM_018853702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJH4"/>
    <dbReference type="STRING" id="869754.A0A1A0HJH4"/>
    <dbReference type="GeneID" id="30026678"/>
    <dbReference type="OrthoDB" id="5377273at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031211">
        <property type="term" value="C:endoplasmic reticulum palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051371">
        <property type="entry name" value="Ras_palmitoyltransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254">
        <property type="entry name" value="GOLGI AUTOANTIGEN, GOLGIN SUBFAMILY A, 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13254:SF0">
        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 7_ERF4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="7">
        <location>
            <begin position="81"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="266" mass="29683" checksum="F5B4B048FC13011B" modified="2016-10-05" version="1">MNDPLAKYAGANTAKHLVFFNYHEYLVSEYRPLNFAEGSPQQNLNSGEDQAHQEHRPALVVSHFPNVYAAYGGELYGRTRIVRVPRIYDLCEELYMVPQFSAYVPGHEPAALTSEAVTEFQVCGAYGGQKFGVTSLTPLVPQWLTKNEFRDIVQTINKLLHEAATPHGKHTWLENMVDFFSGALYSTFYSKYLVASNSELKMKALDAYVESVNISLRAKHPDLQVMSLLKSAMLSLDFQIPMVAGPSAGHLSPRSIHALGEQDKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJJ3</accession>
    <name>A0A1A0HJJ3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714538.1">
        <property type="nucleotide sequence ID" value="XM_018855629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJJ3"/>
    <dbReference type="GeneID" id="30028605"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291857" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="7847" checksum="53CBBD3594B3CE39" modified="2016-10-05" version="1">MTKCFGFFFLSLNPEISGQTSGDSAGQKAGGRDGLIVACTSGMPALDYRLCRVWGRPRVKCSIDSVNSSTGPWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJL4</accession>
    <name>A0A1A0HJL4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_119944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714667.1">
        <property type="nucleotide sequence ID" value="XM_018854139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJL4"/>
    <dbReference type="GeneID" id="30027115"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="107" mass="12009" checksum="54231C4C89CD3F96" modified="2016-10-05" version="1">MSKRNSERGHRCLGVLRNCPLAVGPRHVTPWQGGARERLAVFFLFLRIGISLWTETNRDAPHRVGARPELSAVLYLETLARHYVPGICALMASFFFFVKISTLSPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJN2</accession>
    <name>A0A1A0HJN2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_38250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714687.1">
        <property type="nucleotide sequence ID" value="XM_018856977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJN2"/>
    <dbReference type="STRING" id="869754.A0A1A0HJN2"/>
    <dbReference type="GeneID" id="30029953"/>
    <dbReference type="OrthoDB" id="274828at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017082">
        <property type="entry name" value="Ribosomal_mS29_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036996">
        <property type="entry name" value="RSM23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="428" mass="48332" checksum="65BC2B61DA793D4C" modified="2016-10-05" version="1">MTHLKFGEAVRQLKFENLAASLAPLNLAELSAEHLDNIDSQVVTYNPQTEKVLRDLGSFKKNQNHELFARPVSMVSQNTSRIHADFIEKLSGPSSQNRLCLLGEKGVGKSTLISQTQALLLSNSKQDVVLLHVDFSERMVDGSSDYIFNPVLGKFHQPMFTKRWIKKVRAANEAVFKKMPLTEDVSFVTKKQQYLLKKGEHTLYDYVALNNEFGFFGPSNALQFFIKQLKAHSKTVPVVFSMDNFNSLIGKPHTEYFHKDMKPITLGEFEFGDFVTQLVSGGFSFDKGGLLLSESKDSGECRTLRVGLGLEKPNPYDEPLQCYLEFAEQMTKNGGVKPFYVENMTKPQAREMLLFLDKAGVLQIREYPTKELYKSLEESGAIPGKTINVGEYVRCEDPETQFERILQSSFFASAGNPGRFLKINSLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJR5</accession>
    <name>A0A1A0HJR5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SH3-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_36423</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714741.1">
        <property type="nucleotide sequence ID" value="XM_018856737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJR5"/>
    <dbReference type="STRING" id="869754.A0A1A0HJR5"/>
    <dbReference type="GeneID" id="30029713"/>
    <dbReference type="OrthoDB" id="6250593at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050670">
        <property type="entry name" value="STAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929">
        <property type="entry name" value="JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45929:SF7">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="SH3" evidence="4">
        <location>
            <begin position="100"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="167"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="220"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="299" mass="32526" checksum="F1B1A3BF8BE58555" modified="2016-10-05" version="1">MSAALINRSLTNVRTELEFLYDSEVIDKLLFDSLMLSLPKKYAKDMSPWGLDKVPTKATFDATFTDSIAESLKKTLLRDDSDSSPQTPAPVPLPERTPAPVLGYCQASYDYESSESGDLKLVKGDVLAVVEHLSLDWWKGYKKGSSPAQAGVFPSNYVTLVAEKDFHTSEPSPLPSNNEKATYTPENSSFAAAPPMYKPAPNTAYQQGYQAQPSYGGQFQQPSYGGLVQQPSYGENSQFPPPSTSYNGQQQVQHEQQLPQQNNSGIDPKHVKKFGSKLGNAAIFGAGATIGSGIVNLIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJV0</accession>
    <name>A0A1A0HJV0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphatidic acid phosphatase type 2/haloperoxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_14632</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714757.1">
        <property type="nucleotide sequence ID" value="XM_018854521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJV0"/>
    <dbReference type="STRING" id="869754.A0A1A0HJV0"/>
    <dbReference type="GeneID" id="30027497"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF28">
        <property type="entry name" value="DIHYDROSPHINGOSINE 1-PHOSPHATE PHOSPHATASE LCB3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="258"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="9">
        <location>
            <begin position="81"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="450" mass="49372" checksum="064B2E6C27792C2A" modified="2016-10-05" version="1" fragment="single">KDAGNGPSDHYQTRLPAWRYALRQWCLPIVREETEALARLQAWARTPALDAYFAWTANLASHTFYVLMLPPLFWFGASAMGRDLVFVLGMGVYVTGFCKDLLCLPRPRSPPLQRITMLAYTAQEYGWPSSHSANATAVSLVLLCRLVQMHGLMSTARFAALAALLAGYYFSLLLGRLYCGMHGFFDVGAGVVLGAGLFLFRHVYGAAYDTWLLHSARNASWLGIFATVAMIVAGHLFLVHVHPEPVDDCPCFDDSVAFVGVLLGLDLSHYLCVLTGYFAARNEHADPLLIPFDPSSGALVAAARFAVGVGLVVAWKLLLKPLVFTVLPPLYKRLGIGLSRGHFISTAHTEKTTRQVRRQSLSNMRNEPLADLDKVLSPLTVTMSPETDIDAYELLDYQSHGPLEPGVPVQISGVFRPRYDVEITGRCIIYAGIAGMAVWGLGLSVVLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJW7</accession>
    <name>A0A1A0HJW7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_19983</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714796.1">
        <property type="nucleotide sequence ID" value="XM_018854917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJW7"/>
    <dbReference type="STRING" id="869754.A0A1A0HJW7"/>
    <dbReference type="GeneID" id="30027893"/>
    <dbReference type="OrthoDB" id="273070at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039136">
        <property type="entry name" value="NUFIP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019496">
        <property type="entry name" value="NUFIP1_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309:SF0">
        <property type="entry name" value="FMR1-INTERACTING PROTEIN NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13309">
        <property type="entry name" value="NUCLEAR FRAGILE X MENTAL RETARDATION PROTEIN INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10453">
        <property type="entry name" value="NUFIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="157" mass="18119" checksum="842F41EB85500C38" modified="2016-10-05" version="1" fragment="single">EPVFIEGTNISLQTEEDIARWIAERRKKWPTRKNVEAKTQDQKEKAPEKEQLLSKLNICKFYARNKRCKFGTKCRNVHESGTGQNHQATASQNTSSTRKMINGVLVNIPQRYKKEVQGAGSLFTKLVQRDLYEHENNVIVDFIQYLDSQGVINHDVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK14</accession>
    <name>A0A1A0HK14_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Clathrin adaptor, mu subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_37345</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714629.1">
        <property type="nucleotide sequence ID" value="XM_018856863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK14"/>
    <dbReference type="STRING" id="869754.A0A1A0HK14"/>
    <dbReference type="GeneID" id="30029839"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030131">
        <property type="term" value="C:clathrin adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="6">
        <location>
            <begin position="258"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="190"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="510"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA24148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="627" mass="70033" checksum="C76B3361762136E6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKE6</accession>
    <name>A0A1A0HKE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_37525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714843.1">
        <property type="nucleotide sequence ID" value="XM_018856882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKE6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKE6"/>
    <dbReference type="GeneID" id="30029858"/>
    <dbReference type="OrthoDB" id="506431at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03443">
        <property type="entry name" value="PaaI_thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052061">
        <property type="entry name" value="PTE-AB_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260:SF4">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47260">
        <property type="entry name" value="UPF0644 PROTEIN PB2B4.06"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioesterase" evidence="1">
        <location>
            <begin position="123"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="224" mass="24797" checksum="CE569F87ADE6A8E2" modified="2016-10-05" version="1">MSRLRTFTRVTSPLAAFAAGFLVFPQEWQRGRWEHRHLSYMNKEAVQRIENSEMFKALEADKDTVMSRSSDAFPAQHRKNHVGLGLMAGLGLMEVDPIIFTNSTAGELTAFYHLGKNLISADGQIHNGVTATILDEALCLCGFSQLPSKRGVTANLTIDFHNQAPPESTVVVRAKVVEAKGRKVVIHGTLFTMNSGAGPNVDIASAKCVLVEPKWLKYFWWVPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKH2</accession>
    <name>A0A1A0HKH2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_30769</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715006.1">
        <property type="nucleotide sequence ID" value="XM_018855701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKH2"/>
    <dbReference type="STRING" id="869754.A0A1A0HKH2"/>
    <dbReference type="GeneID" id="30028677"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021858">
        <property type="entry name" value="Fun_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11951">
        <property type="entry name" value="Fungal_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="4">
        <location>
            <begin position="117"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="180"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="862" mass="96877" checksum="50851D90BD3E6FFF" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GWQ6</accession>
    <name>A0A1A0GWQ6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_17449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000013">
        <property type="protein sequence ID" value="OBA16135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709241.1">
        <property type="nucleotide sequence ID" value="XM_018854715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GWQ6"/>
    <dbReference type="GeneID" id="30027691"/>
    <dbReference type="OrthoDB" id="4095080at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043457">
        <property type="term" value="P:regulation of cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044792">
        <property type="entry name" value="TAR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47188:SF1">
        <property type="entry name" value="PROTEIN TAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA16135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="52" mass="6047" checksum="03737BA361AE4C21" modified="2016-10-05" version="1" fragment="single">FPFNNFTYFFTLFSKCFSSFHHCTCSLSVSRQYLALDGIYHPLRAAFPNNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GYZ1</accession>
    <name>A0A1A0GYZ1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Actin interacting protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_136897</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709270.1">
        <property type="nucleotide sequence ID" value="XM_018854413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GYZ1"/>
    <dbReference type="STRING" id="869754.A0A1A0GYZ1"/>
    <dbReference type="GeneID" id="30027389"/>
    <dbReference type="OrthoDB" id="2306at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051016">
        <property type="term" value="P:barbed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032466">
        <property type="term" value="P:negative regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000102">
        <property type="entry name" value="Actin-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19856">
        <property type="entry name" value="WD-REPEATCONTAINING PROTEIN WDR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="82"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="221"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="264"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA16971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="641" mass="69491" checksum="2F673C2E077BF71D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZB5</accession>
    <name>A0A1A0GZB5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="9">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_61985</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709334.1">
        <property type="nucleotide sequence ID" value="XM_018858322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZB5"/>
    <dbReference type="STRING" id="869754.A0A1A0GZB5"/>
    <dbReference type="GeneID" id="30031298"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036261">
        <property type="term" value="P:7-methylguanosine cap hypermethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01720">
        <property type="entry name" value="Sm_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="30"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA17037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="112" mass="13044" checksum="81EAB9C29785BD75" modified="2016-10-05" version="1">MAELIDRVRSELTEIELQQLEEFEFKHGPMSLIQTAMNDGSPVMIYCRNNHKLLAKVKAFDRHCNLILEDVKELWTETLRNSKGKIIKTTSKERFIAKLFLRGDSVIIVLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H1T1</accession>
    <name>A0A1A0H1T1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EF-hand</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_47728</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA17986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709381.1">
        <property type="nucleotide sequence ID" value="XM_018858208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H1T1"/>
    <dbReference type="STRING" id="869754.A0A1A0H1T1"/>
    <dbReference type="GeneID" id="30031184"/>
    <dbReference type="OrthoDB" id="10045710at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF174">
        <property type="entry name" value="GH06923P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="2">
        <location>
            <begin position="50"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA17986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="17500" checksum="1A44385AFDE9FB44" modified="2016-10-05" version="1">MRKKDKRKERKTAFNEDKPWKNHGDLDVISDSQRKRYEGLWVSNKGLYLDRVATKELHGLDCVSMLNLIHGSVVKKLWSRSNLPRETLQAIWDLVDFRKDGTLNKAEFIVGMWLVDQRLYGRKLPKVVNKLVWSSLGGLGVNVVIKKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H278</accession>
    <name>A0A1A0H278_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_80137</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709453.1">
        <property type="nucleotide sequence ID" value="XM_018859176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H278"/>
    <dbReference type="STRING" id="869754.A0A1A0H278"/>
    <dbReference type="GeneID" id="30032152"/>
    <dbReference type="OrthoDB" id="10021397at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17502">
        <property type="entry name" value="MFS_Azr1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1720.10">
        <property type="entry name" value="Multidrug resistance protein D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF198">
        <property type="entry name" value="AZOLE RESISTANCE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="34"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="571" mass="61034" checksum="EC2C9FB448DBC48F" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H295</accession>
    <name>A0A1A0H295_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_47622</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709442.1">
        <property type="nucleotide sequence ID" value="XM_018858196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H295"/>
    <dbReference type="STRING" id="869754.A0A1A0H295"/>
    <dbReference type="GeneID" id="30031172"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF42">
        <property type="entry name" value="UPF0057 MEMBRANE PROTEIN ZK632.10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291703" evidence="7">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA18047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="76" mass="8572" checksum="1FF5A0CAA815A9B1" modified="2016-10-05" version="1">MHARDWFMVFLAVLLPPLPVAVKRGFSMDLLINVCLSIFVYLPGLIHALYIISKYPEPESHVHLGEHDQPQGYGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4N6</accession>
    <name>A0A1A0H4N6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Karyogamy protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_33658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709574.1">
        <property type="nucleotide sequence ID" value="XM_018856369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4N6"/>
    <dbReference type="GeneID" id="30029345"/>
    <dbReference type="OrthoDB" id="10445102at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="305" mass="35120" checksum="D26E193073B235BB" modified="2016-10-05" version="1">MKTSSKAQDLQEQLLAKFQAIVDLMDESFTHQEEQKREEAKTFDDFHNSMLERSRYFEDAWDYMSLEISTNTNKIDQSLSETHQKLQLIFDFVDTQFARILTANENLAKIHVQASQSISDDLMMLYQNSKSGQLVLQDILEDNLHKGLQINYLLQDTHLQVENMFEDIEKETRAIVANVTRELKDEALSLFIVFQTTFESTFSQLMFLSQNIGHIIFSMDAASLNLNSSLVILHERVQDLKSINMSSILSSVMLSPKVVSCVLVAICMFQFMFRWEHGQGAFMSAIFFGAFTTYLARSVIFAMFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4P2</accession>
    <name>A0A1A0H4P2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_47208</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709541.1">
        <property type="nucleotide sequence ID" value="XM_018858135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4P2"/>
    <dbReference type="STRING" id="869754.A0A1A0H4P2"/>
    <dbReference type="GeneID" id="30031111"/>
    <dbReference type="OrthoDB" id="2014058at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008699">
        <property type="entry name" value="NDUFB8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12840:SF1">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 8, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12840">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE ASHI SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05821">
        <property type="entry name" value="NDUF_B8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="175" mass="19937" checksum="2500BE4EC3F11BB8" modified="2016-10-05" version="1">MTLTTRTTITDQSTGETIRLTDPQNPLYADYPKVEPVFALQRDPYATYDFQQLRRNKNDPLTLDDDLYDMWSPDYFQTVDDKTALKHNAIFFSLFFGFGAFIAFWELNPEKPAMPRSYPYNGLAKSLGSGGEATDYFYRVRPDETAAAAGILADDVSVASQEEAYKKAYPDFFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4W9</accession>
    <name>A0A1A0H4W9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_13438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709656.1">
        <property type="nucleotide sequence ID" value="XM_018854362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4W9"/>
    <dbReference type="GeneID" id="30027338"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="201" mass="22453" checksum="C08795C2F7A1E989" modified="2016-10-05" version="1">MNVNQEGSALIDLRYHYYLAPWSASRPTNSITQTIAHTKIQTLVGEILDLQAQSTNYPHDQENVFENESTLLPIPAMAGYAIGNIFLATQYILSLIASNNQNTRKLYFHQYEAGLAGLLGLGILGTVVFEWLLDSKELTSMKEALYWGWNLILTAIFLESCCLLSGWLPLLFCIIVVFVTGFVVGTEYSNCGTMMKTLNQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H591</accession>
    <name>A0A1A0H591_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TPR-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_18941</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709781.1">
        <property type="nucleotide sequence ID" value="XM_018854824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H591"/>
    <dbReference type="STRING" id="869754.A0A1A0H591"/>
    <dbReference type="GeneID" id="30027800"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF2">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="473"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="516"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="482"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="554" mass="61208" checksum="CC48E54B1BC4810E" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5V8</accession>
    <name>A0A1A0H5V8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Got1-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_79858</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GOT1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709872.1">
        <property type="nucleotide sequence ID" value="XM_018859139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5V8"/>
    <dbReference type="STRING" id="869754.A0A1A0H5V8"/>
    <dbReference type="GeneID" id="30032115"/>
    <dbReference type="OrthoDB" id="204784at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000137">
        <property type="term" value="C:Golgi cis cisterna"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="144" mass="15849" checksum="3B927D4AA9A68D45" modified="2016-10-05" version="1">MLFDSTLWLTERQKYGVASTAAGVVFFLLGIVTFFDSSLLAIGNILFIIGVVLIIGPSRALYFFARPTKIKPTLFFFGGIVLILMKHSFIGFGVESVGILFLFGDFFGTIVLFLRSFPIIGPILKHPAIEPYINRVAGLDTLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Z0</accession>
    <name>A0A1A0H5Z0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="15">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_33514</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="15">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="15"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709865.1">
        <property type="nucleotide sequence ID" value="XM_018856317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Z0"/>
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    <dbReference type="GeneID" id="30029293"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:1990417">
        <property type="term" value="P:snoRNA release from pre-rRNA"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="CDD" id="cd17942">
        <property type="entry name" value="DEADc_DDX18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000379">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000460">
        <property type="entry name" value="RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044773">
        <property type="entry name" value="DDX18/Has1_DEADc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="19">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="141"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="18">
        <location>
            <begin position="331"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00024357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA19333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="574" mass="63872" checksum="4DDFF7407D4AFB36" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5Z1</accession>
    <name>A0A1A0H5Z1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">dihydrolipoyllysine-residue succinyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_215011</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710032.1">
        <property type="nucleotide sequence ID" value="XM_018855084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5Z1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5Z1"/>
    <dbReference type="GeneID" id="30028060"/>
    <dbReference type="OrthoDB" id="5391403at2759"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06849">
        <property type="entry name" value="lipoyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000006">
        <property type="entry name" value="Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050537">
        <property type="entry name" value="2-oxoacid_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416:SF5">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43416">
        <property type="entry name" value="DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="14">
        <location>
            <begin position="54"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="128"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="138"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA19504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="420" mass="45950" checksum="590FFAB5A160DB13" modified="2016-10-05" version="1">MLSRTLKSNLRRLPKAAACGAKATPSTIARTLVTKTAAPRLASMPRLTFHRFASTSVKVPEMAESISEGTLSAFNKEVGDFVNQDELVATIETDKIDVEVNAPVSGTITELLVAVEDTVEVGQEIIKIEEGDAPAGGAKKEEAPKQESKDEAPKKEEAPKKEEPKKEAKKEEPKKESKETASFTAFSRTEERVKMNRMRLRIAERLKESQNTAASLTTFNEVDMSNLMDMRKLYKDEILDKTGIKFGFMGAFSKAAALASKEIPAVNASIENNDTMVFRDYMDISIAVATPKGLVTPVVRNAESLSILGVEQEINRLGKKARDGKLSLEDMAGGTFTISNGGVFGSLYGTPIINLPQTAVLGLHGVKERPVTVKGQIVSRPMMYLALTYDHRVLDGREAVTFLKTIKELIEDPRKMLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H619</accession>
    <name>A0A1A0H619_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi apparatus membrane protein TVP38</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Golgi apparatus membrane protein tvp38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_45716</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Golgi membrane protein involved in vesicular trafficking and spindle migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TVP38/TMEM64 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710004.1">
        <property type="nucleotide sequence ID" value="XM_018857952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H619"/>
    <dbReference type="STRING" id="869754.A0A1A0H619"/>
    <dbReference type="GeneID" id="30030928"/>
    <dbReference type="OrthoDB" id="166803at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000022">
        <property type="term" value="P:mitotic spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051076">
        <property type="entry name" value="Golgi_membrane_TVP38/TMEM64"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549:SF1">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47549">
        <property type="entry name" value="GOLGI APPARATUS MEMBRANE PROTEIN TVP38-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="12">
        <location>
            <begin position="89"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="297"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA19476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="328" mass="36089" checksum="DA7B1B1DF96B7829" modified="2016-10-05" version="1">MLQTQVQILNDLGRTSSEWFQSQLTKKKALCIVGGLLGFVAGVTLLVFHKRFIAAMADVSDLIRASKFGGLALFVAIFFVGFPPLLGFSALSMLCGMVYGFPGGWPLLAAASTIGSFASFMVYRYFLRNQAERLVARNEKFRAFAEILKEDASLFLLILLRLCPLPYSLSNGALAAIPNLPASTYLLASVITSPKMFMHVFIGHTIKNLGDAERPAAAKALDVFSILLTGCAVSLASYIIYRRMQQKLESYHERGPNNDAMYEDLVFGDFGESPELGTNLELDAAEFDDDHFIIADDDEDLPAGTNARGLDLLDEPQELGKPPKYRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H651</accession>
    <name>A0A1A0H651_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CREG-like beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_79572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710092.1">
        <property type="nucleotide sequence ID" value="XM_018859090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H651"/>
    <dbReference type="STRING" id="869754.A0A1A0H651"/>
    <dbReference type="GeneID" id="30032066"/>
    <dbReference type="OrthoDB" id="2138282at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055343">
        <property type="entry name" value="CREG_beta-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273:SF1">
        <property type="entry name" value="ADL397C-AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37273">
        <property type="entry name" value="CHROMOSOME 8, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13883">
        <property type="entry name" value="CREG_beta-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291677" description="CREG-like beta-barrel domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="CREG-like beta-barrel" evidence="2">
        <location>
            <begin position="18"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="209" mass="22803" checksum="56196AEF3D003319" modified="2016-10-05" version="1">MRFSLTHVCWASLAWPGVADGAQVARTLVNRELLMNVNTIQQRQGRDAGTAQNGLPVSSMEYYADCDGDGDPYWLVLDIGTPAKNIARGSPYSFTIRAGDHPAQEHVDTRYPGGVASSPAGSPRLTLQGELVAVGASPEALAKLERCFLERHPDAERWLPLNKRSPHQAHWAKVVVSDVFMVGGFGDRAYIGPVAGEQYHAAWIHIEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6R6</accession>
    <name>A0A1A0H6R6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Acyl-coenzyme A oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">METBIDRAFT_206598</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2,3-saturated acyl-CoA + O2 = a (2E)-enoyl-CoA + H2O2</text>
            <dbReference type="Rhea" id="RHEA:38959"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="ChEBI" id="CHEBI:65111"/>
            <dbReference type="EC" id="1.3.3.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; peroxisomal fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 12">Belongs to the acyl-CoA oxidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710246.1">
        <property type="nucleotide sequence ID" value="XM_018854999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6R6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6R6"/>
    <dbReference type="GeneID" id="30027975"/>
    <dbReference type="OrthoDB" id="538336at2759"/>
    <dbReference type="UniPathway" id="UPA00661"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003997">
        <property type="term" value="F:acyl-CoA oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005504">
        <property type="term" value="F:fatty acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033540">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA oxidase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000015">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000003">
        <property type="entry name" value="Acyl-coenzyme A oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055060">
        <property type="entry name" value="ACOX_C_alpha1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029320">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012258">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002655">
        <property type="entry name" value="Acyl-CoA_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909:SF352">
        <property type="entry name" value="ACYL-COENZYME A OXIDASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10909">
        <property type="entry name" value="ELECTRON TRANSPORT OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01756">
        <property type="entry name" value="ACOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22924">
        <property type="entry name" value="ACOX_C_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14749">
        <property type="entry name" value="Acyl-CoA_ox_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000168">
        <property type="entry name" value="Acyl-CoA_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 14" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-coenzyme A oxidase N-terminal" evidence="18">
        <location>
            <begin position="31"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="17">
        <location>
            <begin position="155"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-alpha1" evidence="19">
        <location>
            <begin position="294"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase C-terminal" evidence="16">
        <location>
            <begin position="509"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="683"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="684"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="453"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000168-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF14749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="OBA19721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="703" mass="78545" checksum="141F7363098558E9" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6T5</accession>
    <name>A0A1A0H6T5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_13368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
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        <property type="protein sequence ID" value="OBA19622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_018854346.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052600">
        <property type="entry name" value="Nuc_rcpt_coact/corep"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295:SF6">
        <property type="entry name" value="NEOSIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23295">
        <property type="entry name" value="NUCLEAR RECEPTOR COACTIVATOR 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="335"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="707"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="748"/>
            <end position="839"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
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            <end position="63"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="93"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="603"/>
            <end position="618"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
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            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="767"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="801"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA19622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="839" mass="92301" checksum="FB1CF1FA64AEA927" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Z6</accession>
    <name>A0A1A0H6Z6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_208357</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710331.1">
        <property type="nucleotide sequence ID" value="XM_018855011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Z6"/>
    <dbReference type="GeneID" id="30027987"/>
    <dbReference type="OrthoDB" id="4086710at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="183" mass="19973" checksum="426DBEE5A7C7767A" modified="2016-10-05" version="1">MSRKQQKRQRKNLTVSDGLDEVADLASAVANSVHESTQGLESHEAQNFANFATYPSTSSVLPLSTHGDAKNSLPTFLIKSKEFTQGKLKSVAELQHSISSRRLVYSERTPLLPLHSSPEPHNEETHDEEPQAQSFSSSRAIRLAAGLAIVVSVGFLTVLWLCEEIMIAWRSAGCAVAKLAAFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H766</accession>
    <name>A0A1A0H766_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nuclear transport factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_32878</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710345.1">
        <property type="nucleotide sequence ID" value="XM_018856152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H766"/>
    <dbReference type="STRING" id="869754.A0A1A0H766"/>
    <dbReference type="GeneID" id="30029128"/>
    <dbReference type="OrthoDB" id="6507044at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000005">
        <property type="entry name" value="Nuclear transport factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00026247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="89" mass="10062" checksum="18866FC93197B3C1" modified="2016-10-05" version="1">MLTFETSQLQGAKDIVEKLVSLPFQKVAHRISTLDAQPASPNGDILAMVTGELLIDEEQNAQRYSQVFHLIPDGNSYYVFNDIFRLNYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H771</accession>
    <name>A0A1A0H771_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_208466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710350.1">
        <property type="nucleotide sequence ID" value="XM_018855014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H771"/>
    <dbReference type="GeneID" id="30027990"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA19825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="206" mass="23417" checksum="06FEDF20C4140C96" modified="2016-10-05" version="1">MDPQVASCTERGHEKKGTEQTRERRTHGMQTAHFLENKAHALRRPGAVWPRVFLHVMGPAVLSKWPRLREALHQGLGHSANTRRRETWLLADGKISRQYLQAKTEKTPHVGVFCMDGIFYERRLFYCRGFGKSRVPRPWGQCIQYWASGRGLFHIKGAPAPTNAAAQQARPAWLVWSHGPSTAEDAPWIRMSVQTIMAFHSPPVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7E4</accession>
    <name>A0A1A0H7E4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_44419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710342.1">
        <property type="nucleotide sequence ID" value="XM_018857790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7E4"/>
    <dbReference type="GeneID" id="30030766"/>
    <dbReference type="OrthoDB" id="4089805at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="83" mass="9274" checksum="FCFA687D0EBFD783" modified="2016-10-05" version="1">MAKVLKKVRALPSEPSANDVLKVEYIEVNLNNSRKLINSVTSASSTSQETVKETEAIVEDMLGLRNARAELAWLDKIIDERCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7G5</accession>
    <name>A0A1A0H7G5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_230395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710368.1">
        <property type="nucleotide sequence ID" value="XM_018855250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7G5"/>
    <dbReference type="GeneID" id="30028226"/>
    <dbReference type="OrthoDB" id="4096316at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="2">
        <location>
            <begin position="530"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="671" mass="76153" checksum="9A6914A9EC3D5067" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7H6</accession>
    <name>A0A1A0H7H6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL14m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_45278</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710574.1">
        <property type="nucleotide sequence ID" value="XM_018857902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7H6"/>
    <dbReference type="STRING" id="869754.A0A1A0H7H6"/>
    <dbReference type="GeneID" id="30030878"/>
    <dbReference type="OrthoDB" id="274765at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000005">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005745">
        <property type="entry name" value="Ribosomal_uL14_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01067">
        <property type="entry name" value="rplN_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="132" mass="14571" checksum="F9E233FBE91F4AD6" modified="2016-10-05" version="1">MIYLKTLLKVVDNSGAQIAECINVLRHKPKNCAKVGDQITVVIKEAKPMNSEITGQNANNRVKRRDICRAVVVRTKAPFRRPDGSVVRFDDNACVLINKNGDPLGTRVSSVVAKELKALEYNKVVALAPKVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7I2</accession>
    <name>A0A1A0H7I2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Catabolic 3-dehydroquinase</fullName>
            <shortName evidence="3">cDHQase</shortName>
            <ecNumber evidence="3">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">3-dehydroquinate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DQD1</name>
        <name evidence="8" type="ORF">METBIDRAFT_79360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Is involved in the catabolism of quinate. Allows the utilization of quinate as carbon source via the beta-ketoadipate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; 3,4-dihydroxybenzoate biosynthesis; 3,4-dihydroxybenzoate from 3-dehydroquinate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homododecamer. Adopts a ring-like structure, composed of an arrangement of two hexameric rings stacked on top of one another.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-II 3-dehydroquinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710579.1">
        <property type="nucleotide sequence ID" value="XM_018859045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7I2"/>
    <dbReference type="STRING" id="869754.A0A1A0H7I2"/>
    <dbReference type="GeneID" id="30032021"/>
    <dbReference type="OrthoDB" id="8191625at2759"/>
    <dbReference type="UniPathway" id="UPA00088">
        <property type="reaction ID" value="UER00178"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019631">
        <property type="term" value="P:quinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00466">
        <property type="entry name" value="DHQase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.9100">
        <property type="entry name" value="Dehydroquinase, class II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00169">
        <property type="entry name" value="AroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001874">
        <property type="entry name" value="DHquinase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018509">
        <property type="entry name" value="DHquinase_II_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036441">
        <property type="entry name" value="DHquinase_II_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01088">
        <property type="entry name" value="aroQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003804">
        <property type="entry name" value="PRK05395.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003805">
        <property type="entry name" value="PRK05395.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003806">
        <property type="entry name" value="PRK05395.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003807">
        <property type="entry name" value="PRK05395.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21272:SF3">
        <property type="entry name" value="CATABOLIC 3-DEHYDROQUINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01220">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001399">
        <property type="entry name" value="DHquinase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52304">
        <property type="entry name" value="Type II 3-dehydroquinate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01029">
        <property type="entry name" value="DEHYDROQUINASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 3" id="KW-0672">Quinate metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3 4">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3 4">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <begin position="104"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="3 6">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001399-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA20054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="145" mass="16075" checksum="2482AF6FC063684D" modified="2016-10-05" version="1">MAKKILLVNGPNLNLLGSREPEKYGTTTLADIEAEGQAQVSKYEGAELFTYQNNTEGFIVDRIHEAKKQGVKFIIINAGAYTHTSVAIRDALLGTDIPFIEVHITNVHQREPFRHHLYLLDKAIAVIAGLGVYGYTAALEYALNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7X5</accession>
    <name>A0A1A0H7X5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">GPI transamidase component PIG-S</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32229</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710721.1">
        <property type="nucleotide sequence ID" value="XM_018856012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7X5"/>
    <dbReference type="STRING" id="869754.A0A1A0H7X5"/>
    <dbReference type="GeneID" id="30028988"/>
    <dbReference type="OrthoDB" id="28748at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019540">
        <property type="entry name" value="PtdIno-glycan_biosynth_class_S"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21072:SF13">
        <property type="entry name" value="GPI TRANSAMIDASE COMPONENT PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10510">
        <property type="entry name" value="PIG-S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="57216" checksum="0EC3AA863D73735E" modified="2016-10-05" version="1">MTKTEELLRSKATRVNILLYLSAFVAFLGAPLFFFTTLIHRAHLPAEDVQHRVDEFAQSSQINIPVYFENSQAIPGLIEHTQQAIDASLAQQNLARNWGLTLQSRDAGKIDKLQDYVVRLEEHQTDVNAESINSYKLSKTSKEIGILTHIESLSASDLASYICQVLLEIVFKDELAFISTLLADVPSTDVVFPYSPTYNVVFNLFVEDGRQVDWPIEEAVEFIQPVFDALSNSSTFKPSSQIQYYSKLQSEPILNEDGTARIIPQSDLATFINYGEWNLNTHDIAPSINFLVFFPQSTYANVHLLAESSKTNSFLIPQWGGVHIYNANTKIDRDQKFKFRTAELNLIFDIFASQLFELLGVPNAPLSPALRIAAFHRITTLKNLKRALNNLTALLKLSSSLNGISIPESTREHVLAALENYDIAVQKLHQNDFGAAVAFAGRSVEQSDKAFFEKEMVQQAYFPSEHKLAVFLPLLGPICSIVFFGLLKYLRSRKTEELSSEELKKKQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H806</accession>
    <name>A0A1A0H806_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA20156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710681.1">
        <property type="nucleotide sequence ID" value="XM_018856217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H806"/>
    <dbReference type="STRING" id="869754.A0A1A0H806"/>
    <dbReference type="GeneID" id="30029193"/>
    <dbReference type="OrthoDB" id="10027144at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043577">
        <property type="term" value="P:chemotropism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045141">
        <property type="term" value="P:meiotic telomere clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057778">
        <property type="entry name" value="KH_Vigilin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24668">
        <property type="entry name" value="KH_Vigilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="171"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="552"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="634"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="714"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="795"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="872"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="954"/>
            <end position="1022"/>
        </location>
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    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="1023"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="1135"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="59"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1070"/>
            <end position="1104"/>
        </location>
    </feature>
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        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1204" mass="130974" checksum="6948F96D1BCF297A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
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    <name>A0A1A0H8P7_9ASCO</name>
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        <submittedName>
            <fullName evidence="5">Acid protease</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ORF">METBIDRAFT_44238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
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            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018857764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8P7"/>
    <dbReference type="STRING" id="869754.A0A1A0H8P7"/>
    <dbReference type="GeneID" id="30030740"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Asp"/>
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    </dbReference>
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        <property type="entry name" value="Acid proteases"/>
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        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
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            <begin position="1"/>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="521"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="4">
        <location>
            <begin position="64"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="538" mass="57054" checksum="05324F84B67F6287" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8S7</accession>
    <name>A0A1A0H8S7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_200378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_018854926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8S7"/>
    <dbReference type="GeneID" id="30027902"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="199" mass="22118" checksum="8D3B07C5B336467B" modified="2016-10-05" version="1">MKVMIGIVKSLERSRSQRKKALLLKHKHKLKSLSRNCNLLSLETLSVRDCGSIDKIRIKSTASISLENIPIPSSLPPAYDECIRVQPPTMTGCSRVSDYAEEKIPPTTAPEVCNEITRGRTSQVVYRSQLSGLAKRKSIRRPSPPRNGTKLPLEFGTVDGPVALRVQCCVQSWWKSLAIVQMIGLLVVLMSCFLLVLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U0</accession>
    <name>A0A1A0H8U0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43814</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710937.1">
        <property type="nucleotide sequence ID" value="XM_018857702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U0"/>
    <dbReference type="GeneID" id="30030678"/>
    <dbReference type="OrthoDB" id="10248987at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="63" mass="7184" checksum="A30C023954B9A517" modified="2016-10-05" version="1" fragment="single">KGQIFGGLDVESISILEQNITFDFEKGYVLMRNLIKYVTITIVCDPKDTPIFRHCPSWDIGAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8Y4</accession>
    <name>A0A1A0H8Y4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="13">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_78924</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 13">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the SHMT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="13"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711106.1">
        <property type="nucleotide sequence ID" value="XM_018858968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8Y4"/>
    <dbReference type="STRING" id="869754.A0A1A0H8Y4"/>
    <dbReference type="GeneID" id="30031944"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000097">
        <property type="entry name" value="Serine hydroxymethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF57">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 13" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="14">
        <location>
            <begin position="36"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA20584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="489" mass="53665" checksum="5DC782185BF7C3EA" modified="2016-10-05" version="1">MYSRSISALKSVPRTSLARNYALSAKAQALISKSVREVDPEMALILQQEKDRQRNSITLIPSENFTSKAVMDLLGSEMQNKYSEGYPGERYYGGNEIIDKAESLCRTRALEAFGLDSAKWGVNVQSLSGAPANLYAYSAILEVGDRIMGLDLPHGGHLSHGYQTPSAKISYISKYFQTMPYRLNEETGLIDYDTLEKNAILFRPKVIVAGASAYSRVIDYKRMRAIADKVGAYLLSDMAHISGLVSAGVTDSPFPYSDIVTTTTHKSLRGPRGAMIFFRKGIRKVTKKGKEIPYDLERKINFSVFPAHQGGPHNHTISALAVALKQCSAPEYKEYQQNVVDNAKAFAKALESRGFELVSKGTDTHLILVDLRSKKIDGARVEAVLERANIAANKNTVPGDKSALFPSGLRVGTPAMTTRGFGAEEFGKVAEYFQRAVDIAIDLKKKEQGSVAKELLASFKTLANESAEVKALDEEVKAWASKYPVPGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9C0</accession>
    <name>A0A1A0H9C0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SET domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_43305</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711138.1">
        <property type="nucleotide sequence ID" value="XM_018857642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9C0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9C0"/>
    <dbReference type="GeneID" id="30030618"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19177">
        <property type="entry name" value="SET_SETD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016852">
        <property type="entry name" value="SET_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044429">
        <property type="entry name" value="SETD4_SET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027158">
        <property type="entry name" value="Lys_MTase_YDR198C_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="31"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA20616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="435" mass="49086" checksum="4CA27883E72B7AE4" modified="2016-10-05" version="1">MKNIDEKIDALLSWMGSDGTDSLKSNSHVSAKLEVKDVPGSGRGLYAKAPISRNERLIRILPSYLLNFSTVLAHILKHNPNEQIPASVPQNLHVPVGKKDAVGDIYAGLSLETLLALSSFQIISLFLVLEKSRGSDSFWKPFIDMLPPIEELNLCPLVWKILDLPHADRLWAMLPRSARKHSDSVLARFEKDLLVVQSLLQETALFSIQTFLWAWMCINSRCLYMEIPQAKDSADNLTMAPYVDFLNHLSEDQCGIKIDTLGFQVFTSTGYQSGEELYFSYGAHSNEFLMCEYGFTLGQNKWNYVDITHFISLLFNPKQAQFLKAKGYHGDYTLNIDGMSFRCEVALATLQEQDPETSRRLNSFVEGTTDGSYYERQSNKLLNRILAKLIADCNARLNADHALDHAAQPQIEKIRSLYQDMKQICESAQANHPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9K0</accession>
    <name>A0A1A0H9K0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">DNA replication complex GINS protein PSF2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_43813</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711075.1">
        <property type="nucleotide sequence ID" value="XM_018857701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9K0"/>
    <dbReference type="STRING" id="869754.A0A1A0H9K0"/>
    <dbReference type="GeneID" id="30030677"/>
    <dbReference type="OrthoDB" id="1938138at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21694">
        <property type="entry name" value="GINS_B_Psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1020:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.5.50:FF:000001">
        <property type="entry name" value="DNA replication complex GINS protein PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007257">
        <property type="entry name" value="GINS_Psf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056784">
        <property type="entry name" value="PSF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12772:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25005">
        <property type="entry name" value="PSF2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05916">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028998">
        <property type="entry name" value="GINS_Psf2_subgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="5 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF2 N-terminal" evidence="10">
        <location>
            <begin position="12"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="GINS subunit" evidence="9">
        <location>
            <begin position="75"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR028998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA20553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="195" mass="22656" checksum="4B469CEE3E29D8FA" modified="2016-10-05" version="1">MVLIENLRQNLSPAEIQFLCENEMISILPRYSMPKLDLIADRVPQLKGMKRVEVPLWIALILKGQGKCNIIIPDWLNHDHLSEIYEQERKFPHMFSKLPWNWIELAKIMLTKAADDLQDSSTYLRSILQDLKELRQLKSKRGLRELNESNIQLNGLSLMEINEMRPFVLGVMGKLRELHESTTVEEGEGEGSDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9L3</accession>
    <name>A0A1A0H9L3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_43403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711341.1">
        <property type="nucleotide sequence ID" value="XM_018857652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9L3"/>
    <dbReference type="STRING" id="869754.A0A1A0H9L3"/>
    <dbReference type="GeneID" id="30030628"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040025">
        <property type="entry name" value="Znf622/Rei1/Reh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182:SF8">
        <property type="entry name" value="CYTOPLASMIC 60S SUBUNIT BIOGENESIS FACTOR ZNF622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13182">
        <property type="entry name" value="ZINC FINGER PROTEIN 622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA20819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="50" mass="5752" checksum="CF7986975BBC1A6E" modified="2016-10-05" version="1" fragment="single">MSHTCVSCALEFPDAPAQRAHMKLDWHRYNLKRRVAQLPAISEAVFVDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA42</accession>
    <name>A0A1A0HA42_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DUF618-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_42321</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711509.1">
        <property type="nucleotide sequence ID" value="XM_018857530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA42"/>
    <dbReference type="STRING" id="869754.A0A1A0HA42"/>
    <dbReference type="GeneID" id="30030506"/>
    <dbReference type="OrthoDB" id="10069473at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460:SF0">
        <property type="entry name" value="CID DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12460">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE INHIBITOR-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00582">
        <property type="entry name" value="RPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="3">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="299"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="235"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="384"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="405"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="423"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="470" mass="51303" checksum="16EA50021104B442" modified="2016-10-05" version="1" fragment="single">SMSFSPEIFRNKLDALQETQDSIVSLSQWVLFHHRHASHLCELWADYALQTSAGAPLKKKLSLLYLCNDVVQQARHKRKPEFIAGFAARLPAVLHALYGAVDEPVKPKVERLISVWNERSVFTKPQIENMQHAVLQAKDGQAFDPADAPDAGLKALLKAAAPPRIAPQLAPVNGLFLHMAQLLATASANVAHAHQQAQLYLPDDPSALDALPLPKVYVSKLNVLEKLCLMTVQTLEDAQTARRDIVAALADLSREVLAGLPPDTLQIELLQQKLERVRSTRAELLDMVDELLLQGVNSSVEHPSPAEEPSPVFDTSPDTAPSASDSLVPTYEDSSDSDSDADLPPAKRVAALLDEPPAQKKRRVSDASLSSATSKKSVAFSEEIEVKEFERDDQANYMDIIGDAGADDDDDDDNNNLPANDTHGFEIHHKDAVELMHERESEPGPGHSDESLSAPDPDKGDLLSLLSKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA49</accession>
    <name>A0A1A0HA49_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polynucleotide 5'-hydroxyl-kinase GRC3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polynucleotide 5'-hydroxyl-kinase grc3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CLP1</name>
        <name evidence="12" type="ORF">METBIDRAFT_72015</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of a pre-mRNA cleavage factor complex. Interacts directly with PCF11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Clp1 family. Clp1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711270.1">
        <property type="nucleotide sequence ID" value="XM_018858403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA49"/>
    <dbReference type="STRING" id="869754.A0A1A0HA49"/>
    <dbReference type="GeneID" id="30031379"/>
    <dbReference type="OrthoDB" id="258143at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005849">
        <property type="term" value="C:mRNA cleavage factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03035">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028606">
        <property type="entry name" value="Clp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="10">
        <location>
            <begin position="23"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="11">
        <location>
            <begin position="142"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="9">
        <location>
            <begin position="351"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="145"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA20748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="457" mass="49753" checksum="8ECC66739DA858BC" modified="2016-10-05" version="1">MSIPGFAASAAFEEPTLAVEVTIPARAEWRFECPFKLVMKVKIIEGVAEIFGTELPNDVEVRLTGCKYAIYAPLAAGCRLQYEVGPNKEELNISNEDAKISEYLSDDSPALQYTNAHFALEVMRHEAAASTDRNGPRVLVLGSSQSGKTALAKTLAAYAVKMDRVPLFVNLDPKTGVFSVPGSLTATPISDAFDLESVNGWGGSTTSGATYHNPKQPLVKSFGFYEFSENVDLYKHQVAQLGIACLSRASQDDQIRTGGLIVDTPSLSMKDAAVVENIVADFDINIILVTGSDRLAVDLKRKFAHKIARQTLDVVKMARNSAVAEVDDSFVRKAQEDTIKEYFNGNFKTRLSPYKTDVDMKNYTIYKTVKLSEYASQMAFLPAGDSSNLENSIIAITQAPVPANGKIAPRDLLNASVLGYAHVSKVDDAKCRMSLLLPFPGQIPRNVLIATSIGYTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAB3</accession>
    <name>A0A1A0HAB3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcriptional coactivator HFI1/ADA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_78147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711579.1">
        <property type="nucleotide sequence ID" value="XM_018858831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAB3"/>
    <dbReference type="STRING" id="869754.A0A1A0HAB3"/>
    <dbReference type="GeneID" id="30031807"/>
    <dbReference type="OrthoDB" id="10264870at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd22933">
        <property type="entry name" value="HFD_HFI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024738">
        <property type="entry name" value="Hfi1/Tada1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21277:SF5">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12767">
        <property type="entry name" value="SAGA-Tad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="310"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="321"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="390" mass="42565" checksum="14FEF158FEC02979" modified="2016-10-05" version="1">MSSVAAPSPVAASQPAKTNKRLQLEAVMREFQSKLGPDWETYHEALSAFLIGRLSRAELVARVGPLMKAGLLKYHNKLLLLNFAHSLQDGAQEYLSELASFWNKRNAKARGVKLSQYEKFKQNIMGLPLKERRRIRAITKDAGKRDRLGASVTLTRQALLPKIPMILDKEQQQLQVNNLVQWQQDVVNGINTPLASASHELPDTDTLSRKVLMTSREHGLTGGIGARVFEVISLGLETHLKNIVECAVDSVRYRKRKYESSAFLQPGFLQGGAGDMYNTLEMFPHLAEPGGPQARLCSIMLQNDDLIDHAGPGYELPPRPALAAPKAAPSAPESAGPKRPALDPENGPAAQGNGVVPNGGSGAPPPKEVSHVGTPDELKWLLHDLIRSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAK9</accession>
    <name>A0A1A0HAK9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_167073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711423.1">
        <property type="nucleotide sequence ID" value="XM_018854666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAK9"/>
    <dbReference type="GeneID" id="30027642"/>
    <dbReference type="OrthoDB" id="10479627at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="147" mass="16244" checksum="9EEC275523965092" modified="2016-10-05" version="1">MSSLSFLDHQLTLCDQIFDRAVLLGAQLESAISVVLGAAIVAITVPLITMFALDFGAYVSTLAARTLEHNFLAVRRPEIEFFRTSTSIGLVFKMDSLSAIRNLVNSNIRKCGSRSLMRIEVVDSTRQSWPLPCDLSQAVQIWQPNGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAV1</accession>
    <name>A0A1A0HAV1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <ecNumber evidence="3">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_11601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrpF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711523.1">
        <property type="nucleotide sequence ID" value="XM_018854030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAV1"/>
    <dbReference type="STRING" id="869754.A0A1A0HAV1"/>
    <dbReference type="GeneID" id="30027006"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="9">
        <location>
            <begin position="71"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA21013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="251" mass="27375" checksum="562A917659B5FCA5" modified="2016-10-05" version="1">MPPLVKICGLRSTEAAQKAIDSGADMLGVIMVPNRKRTVSPGVALEIAKACKKKRKELGRQFQTAGELTTHLSSQKFASHIEYFEAAARLVHENGPFLVGVFRNQGIEDVFGLARDCAVDIIQLHGSEEAQGYLRYNDTHGFGIIKRYVIPDHVDLMAQFFPHIARAERGFLLPLLDSEAGGEGKTIEWPLINGLDGRFLLAGGLDPSNVAETRPFLRNVVGFDVSGGVEDGDGQKNMAKIEAFVANAKQM</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBF5</accession>
    <name>A0A1A0HBF5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Monothiol glutaredoxin-5, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_78255</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711728.1">
        <property type="nucleotide sequence ID" value="XM_018858851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBF5"/>
    <dbReference type="STRING" id="869754.A0A1A0HBF5"/>
    <dbReference type="GeneID" id="30031827"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000005">
        <property type="entry name" value="Glutaredoxin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF16">
        <property type="entry name" value="GLUTAREDOXIN-RELATED PROTEIN 5, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="7">
        <location>
            <begin position="40"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="16691" checksum="1B7AFA27E4A603FA" modified="2016-10-05" version="1">MFSRSIGQASSRLFRQPLFFRSLSTEVKNALDGAVRSAPVVLFMKGTPEFPQCGFSRATIQILGQQGVDPNKFAAYNVLDDPELREGIKEYSQWPTIPQLYVNKEFIGGCDIVQSMAQSGDLLELLESNDCLVAEEDESPQQADIKPSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBI9</accession>
    <name>A0A1A0HBI9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_182111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711886.1">
        <property type="nucleotide sequence ID" value="XM_018854780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBI9"/>
    <dbReference type="GeneID" id="30027756"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="129" mass="14902" checksum="CE34413ACEDFADF8" modified="2016-10-05" version="1">MLSCGKKGPDRRFFMPGYHGLLRFARFRPMRPRSWCILNHVGRMMRLKTKHYSDDRKMLDHPCRGFHKASTDIAFGNPLTAAHCFTLDMNEKHQIQNVLVLIFSREASPCNLQKTTGFIDPGSLSLSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBN1</accession>
    <name>A0A1A0HBN1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Noc2-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_32052</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712054.1">
        <property type="nucleotide sequence ID" value="XM_018855975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBN1"/>
    <dbReference type="STRING" id="869754.A0A1A0HBN1"/>
    <dbReference type="GeneID" id="30028951"/>
    <dbReference type="OrthoDB" id="10266662at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="710"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="710"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="721"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="746" mass="84062" checksum="7AE87316D9CABF39" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBX8</accession>
    <name>A0A1A0HBX8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_42598</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712026.1">
        <property type="nucleotide sequence ID" value="XM_018857559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBX8"/>
    <dbReference type="STRING" id="869754.A0A1A0HBX8"/>
    <dbReference type="GeneID" id="30030535"/>
    <dbReference type="OrthoDB" id="2192946at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="8">
        <location>
            <begin position="59"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="7">
        <location>
            <begin position="527"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="672"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="678"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="860" mass="97034" checksum="8E8B1A76539783D6" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC16</accession>
    <name>A0A1A0HC16_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_41095</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711943.1">
        <property type="nucleotide sequence ID" value="XM_018857353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC16"/>
    <dbReference type="STRING" id="869754.A0A1A0HC16"/>
    <dbReference type="GeneID" id="30030329"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008521">
        <property type="term" value="F:acetyl-CoA transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035348">
        <property type="term" value="P:acetyl-CoA transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000289">
        <property type="entry name" value="Acetyl-coenzyme A transporter 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024371">
        <property type="entry name" value="AcetylCoA_trans_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778:SF9">
        <property type="entry name" value="ACETYL-COENZYME A TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 33 ACETYL-COA TRANSPORTER -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13000">
        <property type="entry name" value="Acatn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA21433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="534" mass="60274" checksum="57B46BFA55AEF1E0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC78</accession>
    <name>A0A1A0HC78_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">D-lactate dehydrogenase (cytochrome)</fullName>
            <ecNumber evidence="6">1.1.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-lactate ferricytochrome C oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_32198</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-lactate + 2 Fe(III)-[cytochrome c] = 2 Fe(II)-[cytochrome c] + pyruvate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13521"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.1.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-binding oxidoreductase/transferase type 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.2.4" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712244.1">
        <property type="nucleotide sequence ID" value="XM_018856002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC78"/>
    <dbReference type="STRING" id="869754.A0A1A0HC78"/>
    <dbReference type="GeneID" id="30028978"/>
    <dbReference type="OrthoDB" id="7786253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008720">
        <property type="term" value="F:D-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903457">
        <property type="term" value="P:lactate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.45.10:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2740:FF:000001">
        <property type="entry name" value="D-lactate dehydrogenase mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="entry name" value="Vanillyl-alcohol Oxidase, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-bd_oxidored_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748:SF117">
        <property type="entry name" value="AER321WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11748">
        <property type="entry name" value="D-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="FAD-linked oxidases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="10">
        <location>
            <begin position="123"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA21734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="558" mass="61963" checksum="846EED650D64ACD2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCC2</accession>
    <name>A0A1A0HCC2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DUF159-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_78550</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712156.1">
        <property type="nucleotide sequence ID" value="XM_018858897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCC2"/>
    <dbReference type="STRING" id="869754.A0A1A0HCC2"/>
    <dbReference type="GeneID" id="30031873"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="324"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="329"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="372" mass="42300" checksum="09C42DBF013F0A64" modified="2016-10-05" version="1">MCGRYALGISIENLPQQFNENVLHPDQSTDLTEQTDHEIAPNSLLEKRYETTASVSGTECTIHLNVLSSGFQASYNIPPTATGAIIYMDKHNSQNNEQENIIYCMEPLKFGLLPVWAKPQNSQSVKKGTGKTHGTEYSREVQQHQAKLFNCRRETLAQAQTVWATPRKHSRCVVPILGYFEWLNTEQGKVPYFVHLPTSPLLFLAGLYSHNHNYNDTEMVPLGAKHFSSFSIVTGPASGKGKNDMLWLHSRKPIFIEPNSKAWFDWLRPDDVWDEQILQSCLNTDTNPVYESLRWHTVAKSVGNPKNKCLDVIEEENVKQRPISSFFQTKKEAKPEPKSEGIAKCDDTGESSLTRKRKLDSDEEKTKKMKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCD0</accession>
    <name>A0A1A0HCD0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_12134</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712160.1">
        <property type="nucleotide sequence ID" value="XM_018854175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCD0"/>
    <dbReference type="GeneID" id="30027151"/>
    <dbReference type="OrthoDB" id="4096434at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="359" mass="40955" checksum="7BD28E17E2261FF9" modified="2016-10-05" version="1">MFYDAPVIPSSPCTISKACWEDIMGDTYSYEHGMIYYNNALKESTPRTENVSMASQVPYSPVIKQESDCGYSQTEYTYISSSDFLCMTPANTSYTSNSKTLSWESLFDEDSRIILDIQIPQPPPDHQNTQTETVDSFQSFTTASMKMVNQNVRGSFAICSDLAAFMEPKQKLLTSAYLVTLNFTKLKSFLGIISLSGPAQEYRLPIFKKIESLDDMLQSGKYDIPKTALDTGPKQVLPALKNRYVSRLTRRQLALVLGIHNYDISLVKDIENVILAMCDQLCGLKIGESSWSRIPRLARKDAVLKVYRFATPFFPNFTMDNVLTIIKRGAYSRTQEFLRRKRRRERKYSIEQNMSAMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCK4</accession>
    <name>A0A1A0HCK4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712235.1">
        <property type="nucleotide sequence ID" value="XM_018857564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCK4"/>
    <dbReference type="STRING" id="869754.A0A1A0HCK4"/>
    <dbReference type="GeneID" id="30030540"/>
    <dbReference type="OrthoDB" id="4087202at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026241">
        <property type="entry name" value="GIP4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02082">
        <property type="entry name" value="GLC7IP4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="509"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="838" mass="94004" checksum="630CB717886930BA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCX8</accession>
    <name>A0A1A0HCX8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_15072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712367.1">
        <property type="nucleotide sequence ID" value="XM_018854563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCX8"/>
    <dbReference type="GeneID" id="30027539"/>
    <dbReference type="OrthoDB" id="2163387at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="370" mass="38825" checksum="A2ACAC40CC20D4A1" modified="2016-10-05" version="1" fragment="single">KTPLKKEELEKIAQELKKKLSRASVAAKQLLSATATGAGRSLLPDSPVLQTLLPKLSPLKNYYMWRRQIAGATAKNVTLLPVLYSPGRRLPTLTKPSIFLSSSPLKNFNAGLSPAEPSPAGALSRKASMDSEALTELNDSPPHMVLEEIHGPQTPPKREALHPLPVLSSNPPSGRRTGPKPALTTPTLAKKPLSTGPNALLKTPTQAARGDAYNDDEGADLLMYLAASPSVPKSGAANTPRAPHNVAFAGHPGASKPHSGSLFMAPPLTPKRPLISTAKTPQNRLTPSVYGGLAVPGSTLPSAGLALTPAGFNMNDYVNFFTPSPGTAAAAHGGLAGRTLLKTPDFHGQVQSAHTSVDGKMINFDKAELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD53</accession>
    <name>A0A1A0HD53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRN6 beta-propeller domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_10817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712396.1">
        <property type="nucleotide sequence ID" value="XM_018853858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD53"/>
    <dbReference type="STRING" id="869754.A0A1A0HD53"/>
    <dbReference type="GeneID" id="30026834"/>
    <dbReference type="OrthoDB" id="4090074at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070860">
        <property type="term" value="C:RNA polymerase I core factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001179">
        <property type="term" value="F:RNA polymerase I general transcription initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001163">
        <property type="term" value="F:RNA polymerase I transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042790">
        <property type="term" value="P:nucleolar large rRNA transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019350">
        <property type="entry name" value="RNA_pol_I-sp_TIF_RRN6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048535">
        <property type="entry name" value="RRN6_beta-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28221">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28221:SF2">
        <property type="entry name" value="RNA POLYMERASE I-SPECIFIC TRANSCRIPTION INITIATION FACTOR RRN6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10214">
        <property type="entry name" value="Rrn6_beta-prop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRN6 beta-propeller" evidence="2">
        <location>
            <begin position="285"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="742"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="761"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="806"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="821"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="845"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="871"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="892"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA21900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="903" mass="100777" checksum="D89610F6672CFF45" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD70</accession>
    <name>A0A1A0HD70_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_139323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712374.1">
        <property type="nucleotide sequence ID" value="XM_018854454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD70"/>
    <dbReference type="GeneID" id="30027430"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="132" mass="15655" checksum="EB4F4A7D5B76B194" modified="2016-10-05" version="1">MSISGRCGSNPRCRLSRRNSWASPVQFLTVGLVLPWVRFIVCLFLIYGFDVFVWRSNGYWGRASNRSWMNQNENWQGRPKKYVKKALEGQLDYCDVYWEMVNCKKMGRHLLKIRRDNCIDEKPTFGKYESKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD89</accession>
    <name>A0A1A0HD89_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Ribosome biogenesis protein NOP53</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_10887</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the NOP53 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712477.1">
        <property type="nucleotide sequence ID" value="XM_018853873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD89"/>
    <dbReference type="STRING" id="869754.A0A1A0HD89"/>
    <dbReference type="GeneID" id="30026849"/>
    <dbReference type="OrthoDB" id="5072at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011687">
        <property type="entry name" value="Nop53/GLTSCR2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211">
        <property type="entry name" value="GLIOMA SUPPRESSOR CANDIDATE REGION GENE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14211:SF7">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NOP53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07767">
        <property type="entry name" value="Nop53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017302">
        <property type="entry name" value="Gltscr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="275"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR017302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="393" mass="44846" checksum="63086B8218BB6C25" modified="2016-10-05" version="1">MDAKTKTQSLRKGKKAWRKNILVDDINLALDANRERQVLHGDDTDGDFVIDTEGSSRPKNAVSVLKTTEILANKSKVPALASRKTKLKVSKARAEKLMILAGRLATGSKGKARTDTEGLVKGSNVDLWAEDDTSASKKINEPTRISFTKASKAPATLKHQPLHLHTEAEVDDLVHAGKSYNPSVEAWKSLVDREYVLENDAQMKVQALEQEQERIQHLMETLKDDELDGGESSAEEDVAEPEDPEEKYRLSVNERTEVKRKTRTQKNREARHKQQLELLKKMRELKSQLRDLQNLLAIQQEVAEKAALQDQKPRVPKKYKSHSKHEPLFTPIEVKLLDELTNNLRAVKPEGNPFYEQMYKLQMSGKVEARVPVAPRKRYKQKLTEKWSYKQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDC7</accession>
    <name>A0A1A0HDC7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_141618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712429.1">
        <property type="nucleotide sequence ID" value="XM_018854484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDC7"/>
    <dbReference type="STRING" id="869754.A0A1A0HDC7"/>
    <dbReference type="GeneID" id="30027460"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="288" mass="31085" checksum="AF7C7A7D71FA50EF" modified="2016-10-05" version="1">MSLWVFLSYDTYLCCVNSLPSGTSLFWDFLPFGSLCPGISYLLGFLAFCLLDFLPFGFHAFWKSLSGTHPNYAPLCLLGFLAFWVSCLLETTYPSYAPFCLLGLAIATRHGPRTTIATADAAGDAAIATTGLPATVRAAKKRQLQSRIRLLQPVPADSAAVPQQAAAGTLPAQFGYPSFDYSAYGQVNQTPLGSMASPGYYQVNTAKTAIPSAAYPFPLVLSSREHCLCTMDWMERRDGLEDSVLLFHPRIVDADHGLIAHADQTARLNHPSAFASKVIGNPNSSNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDC9</accession>
    <name>A0A1A0HDC9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_77445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712398.1">
        <property type="nucleotide sequence ID" value="XM_018858703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDC9"/>
    <dbReference type="GeneID" id="30031679"/>
    <dbReference type="OrthoDB" id="8189076at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="148" mass="16528" checksum="FBEE6939CC59E714" modified="2016-10-05" version="1">MATLPEQIKIDDITSGRVDPQVIYAELEHLKSEINILRSDMSVFLKALATIPESQSQQVYYDNLLAKVLTIRLDIKGFCAQYNRLLPIINLTQIKLGHEVEVLSGLSNGTTNGKANVRLSVPRPHLIHALPEATNRTSKNTETHNKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDR4</accession>
    <name>A0A1A0HDR4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_77600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712616.1">
        <property type="nucleotide sequence ID" value="XM_018858730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDR4"/>
    <dbReference type="STRING" id="869754.A0A1A0HDR4"/>
    <dbReference type="GeneID" id="30031706"/>
    <dbReference type="OrthoDB" id="6585768at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040001">
        <property type="term" value="P:establishment of mitotic spindle localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904750">
        <property type="term" value="P:negative regulation of protein localization to nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031536">
        <property type="term" value="P:positive regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023056">
        <property type="term" value="P:positive regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902542">
        <property type="term" value="P:regulation of protein localization to mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd04128">
        <property type="entry name" value="Spg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000330">
        <property type="entry name" value="Septum-promoting GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017231">
        <property type="entry name" value="Small_GTPase_Tem1/Spg1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037527">
        <property type="entry name" value="Small_GTPase_Tem1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="197" mass="22560" checksum="DB186EC8597CD62F" modified="2016-10-05" version="1">MQHEQPSNQVALKVGLIGDSQIGKTSLMVKYVEGLFDEDYIQTLGVNFMDKKILIRSTNITFLIWDLGGQKEFINMLPLVSNDAVAILFMFDLTRKLTLNSIKEWYRQVRGFNKTAIPFLIGTKYDEFIDLLPQDQLEITHQAKKFGKAMKLPVVFCSTSHSINVQKIFKIILSKAFDLKLNLEEIVNVGEPILIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE28</accession>
    <name>A0A1A0HE28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycosylphosphatidylinositol anchor biosynthesis protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_31168</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712731.1">
        <property type="nucleotide sequence ID" value="XM_018855786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE28"/>
    <dbReference type="STRING" id="869754.A0A1A0HE28"/>
    <dbReference type="GeneID" id="30028762"/>
    <dbReference type="OrthoDB" id="17366at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009580">
        <property type="entry name" value="GPI_biosynthesis_protein_Pig-F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06699">
        <property type="entry name" value="PIG-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA22235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="235" mass="25819" checksum="B37E14E1AF32591A" modified="2016-10-05" version="1">MRRRSIKKTVSFQTQVPGTTDGQIPEPGVLEGSVSIFAVPIHLLLLLHSLFSSGLTENPTQMMAKGLVNLVGMQILYGFWIVYFEKPSIKSKNKATSKGSAGSNIVLVLSCIAISVLLANITFVALILLGAPMHGFLKETYLLACHASVIVVHPILILFRLDLNQLGLIFKNEQVFRRIFRNQTLSASFVAILGAWIGVIPIPLDWDRPWQQWPITILSGVYVGSFAGSLTSVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE29</accession>
    <name>A0A1A0HE29_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclic-AMP phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38704</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclic nucleotide phosphodiesterase class-II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712654.1">
        <property type="nucleotide sequence ID" value="XM_018857035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE29"/>
    <dbReference type="STRING" id="869754.A0A1A0HE29"/>
    <dbReference type="GeneID" id="30030011"/>
    <dbReference type="OrthoDB" id="258495at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047555">
        <property type="term" value="F:3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006198">
        <property type="term" value="P:cAMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902660">
        <property type="term" value="P:negative regulation of glucose mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07735">
        <property type="entry name" value="class_II_PDE_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000396">
        <property type="entry name" value="Pdiesterase2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28283:SF1">
        <property type="entry name" value="3',5'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02112">
        <property type="entry name" value="PDEase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000962">
        <property type="entry name" value="Cyc_nuc_PDEase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00388">
        <property type="entry name" value="PDIESTERASE2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0114">cAMP</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR000962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="370" mass="40869" checksum="2EE2F2BDD236217F" modified="2016-10-05" version="1">MSYEITFIGTSGGPLESGNCSILVKPASLTYSQVLQGRDRPLFMIDAGSGWHALAETILRDRTKSGGSTEAPSRFSTQMALYEDCGTPQEYLQIPHSQPFLDLKDPPLIALRRVFSKVAAMLLSHPHMDHVKAMVINLAGFSDAGEKTVIYASKFTTDALNKHVFNGVMWPDMVLLGLVDLRPIPERGVLSMASGAYSVKHFDLSHGSVVNNGQYIEPYMSLAFLVCDNTSRAKMLIFGDFEADSILRLERNSLIWMYVAPFVLDGSLKCVILECSTHSREPGINLYGHMTPQHVMAELQRLETACAQTNEGSATAPFVRDLKVIVTHVKETYDGKDPRRRIMKELCELNDTQGLRLSISIATNGVLVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB8</accession>
    <name>A0A1A0HEB8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mitochondrial carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_31180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712744.1">
        <property type="nucleotide sequence ID" value="XM_018855795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEB8"/>
    <dbReference type="GeneID" id="30028771"/>
    <dbReference type="OrthoDB" id="193856at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000064">
        <property type="term" value="F:L-ornithine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF57">
        <property type="entry name" value="MITOCHONDRIAL SUBSTRATE CARRIER FAMILY PROTEIN L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="8"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="104"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="198"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA22248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="289" mass="32425" checksum="396AF905CCDF0112" modified="2016-10-05" version="1">MKEGDKAPPGYLGFVAGMFSGVTKNAVGHPFDTVKVRLQTSEGRFKGPVDCVMSTFKAEGVRGFYKGFTPPLAGWVLMDSVMLGSLHLYRRLLKENIYSDEKKLPLFGHIVAGLGSGWTVSLVAAPIEQFKARLQVQYDAKSSNYSGPIDVARKLWKISPLTWYTGLIPTMIFRTNFIFWWGSYEILTNWFTTNTKMSTPSINFWAGGLSATVFWIFAYPTDVVKQVIMTDSPIQAEKRFPRYMDAVRYIYKERGLAGFTRGFGPSILRSFPANAAALAAFEAVMRFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEF2</accession>
    <name>A0A1A0HEF2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Cap-associated protein CAF20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_38987</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Acts as an inhibitor of cap-dependent translation. Competes with eIF4G1 and EAP1 for binding to eIF4E and interferes with the formation of the eIF4F complex, inhibiting translation and stabilizing mRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CAF20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712861.1">
        <property type="nucleotide sequence ID" value="XM_018857076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEF2"/>
    <dbReference type="STRING" id="869754.A0A1A0HEF2"/>
    <dbReference type="GeneID" id="30030052"/>
    <dbReference type="OrthoDB" id="3995390at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008190">
        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031456">
        <property type="entry name" value="Caf20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17052">
        <property type="entry name" value="CAF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0652">Protein synthesis inhibitor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="165" mass="18902" checksum="D254DA9C90744D89" modified="2016-10-05" version="1">MPKYTEEQLLEARELAFTPKPDVLESFSKLVESVREHVANEKRVKITNGDSYIDEHGHERSYNHFNRRRLSRGNGPKPNLRKKSETTVDEDGWETYVKPRKSFGGDDHADDRTAFRELAKDTTVRVRPNNKNLGSSKAVDPRDAIADKHTNTFNAFEALGDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEG3</accession>
    <name>A0A1A0HEG3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone acetyltransferase</fullName>
            <ecNumber evidence="3 12">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_31214</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 12"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712789.1">
        <property type="nucleotide sequence ID" value="XM_018855811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEG3"/>
    <dbReference type="STRING" id="869754.A0A1A0HEG3"/>
    <dbReference type="GeneID" id="30028787"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990467">
        <property type="term" value="C:NuA3a histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="14">
        <location>
            <begin position="288"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="729"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="734"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="782"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="825" mass="94111" checksum="59C3D22E2933B8EE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEI4</accession>
    <name>A0A1A0HEI4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_11168</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712808.1">
        <property type="nucleotide sequence ID" value="XM_018853924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEI4"/>
    <dbReference type="STRING" id="869754.A0A1A0HEI4"/>
    <dbReference type="GeneID" id="30026900"/>
    <dbReference type="OrthoDB" id="29558at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="169" mass="18946" checksum="BB105D435AD29928" modified="2016-10-05" version="1">MGKKVNLNSVAELRLAVDEALPELFQQLGYEQSFALGDLKLALGFSTVAIAGGLYYLEKNVSFNDSYYVIVALIALYAVISAAMYVLSNGKGYKNNKYIGFNEKKQKISVYALAEPFDPIYDVKVVINDNFSAPVTESIPFAKMFDAFGFLNHEESKQIFRELLEKKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEL8</accession>
    <name>A0A1A0HEL8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_39361</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the major facilitator superfamily. Allantoate permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712942.1">
        <property type="nucleotide sequence ID" value="XM_018857132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEL8"/>
    <dbReference type="STRING" id="869754.A0A1A0HEL8"/>
    <dbReference type="GeneID" id="30030108"/>
    <dbReference type="OrthoDB" id="4454541at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000064">
        <property type="entry name" value="MFS allantoate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF97">
        <property type="entry name" value="ALLANTOATE TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G14700)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="423"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="447"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="66"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA22446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="514" mass="56996" checksum="5CC75EC86DBF4BFA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HET4</accession>
    <name>A0A1A0HET4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 12">FACT complex subunit POB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">METBIDRAFT_77889</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 12">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a stable heterodimer with SPT16. The SPT16-POB3 dimer weakly associates with multiple molecules of NHP6 to form the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the SSRP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713007.1">
        <property type="nucleotide sequence ID" value="XM_018858786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HET4"/>
    <dbReference type="STRING" id="869754.A0A1A0HET4"/>
    <dbReference type="GeneID" id="30031762"/>
    <dbReference type="OrthoDB" id="498543at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031508">
        <property type="term" value="P:pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045899">
        <property type="term" value="P:positive regulation of RNA polymerase II transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000098">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 12" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="363"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="467"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="471"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="OBA22511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="540" mass="60590" checksum="9AA1651E8EF20E8C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEV0</accession>
    <name>A0A1A0HEV0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_154826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713022.1">
        <property type="nucleotide sequence ID" value="XM_018854601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEV0"/>
    <dbReference type="GeneID" id="30027577"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="116" mass="12759" checksum="6CB285128051C377" modified="2016-10-05" version="1">MIKHPDQTETKTDQTETDQTETDQKKPNHYKSIKLEKKKEKKKKKSTEKEPLLAVPSLPTALEFRAPLTSKPMPAAPAPSLPASSASGEPAHSALGHRPRQLRPTSNRPMACAPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFA8</accession>
    <name>A0A1A0HFA8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713305.1">
        <property type="nucleotide sequence ID" value="XM_018855396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFA8"/>
    <dbReference type="GeneID" id="30028372"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="186" mass="21087" checksum="33140711C04CB9EF" modified="2016-10-05" version="1">MTFNPSRGKTSSPSNSQRKHSSLRNISSGIKSFVLRDDKAPKEPDNKESVSRLAIVEQNTRHHGIVTIQAKCKGKRSRFKNFQMDPVPESHESAPEAPLLEDLSVVESKIHSKTVRSNRPSSRPMQTPAENTTPKKCTELREANVTSIDLAARTDHRTIPKVKKYPRHFPFKNGKLEQTTKAWKDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFI3</accession>
    <name>A0A1A0HFI3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP-dependent RNA helicase DHH1</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">ATP-dependent RNA helicase dhh1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">METBIDRAFT_40419</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713119.1">
        <property type="nucleotide sequence ID" value="XM_018857269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFI3"/>
    <dbReference type="STRING" id="869754.A0A1A0HFI3"/>
    <dbReference type="GeneID" id="30030245"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098562">
        <property type="term" value="C:cytoplasmic side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036267">
        <property type="term" value="P:invasive filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045900">
        <property type="term" value="P:negative regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010603">
        <property type="term" value="P:regulation of cytoplasmic mRNA processing body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000749">
        <property type="term" value="P:response to pheromone triggering conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000114">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000364">
        <property type="entry name" value="ATP-dependent RNA helicase DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 17" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="61"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="241"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="412"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="16">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="432"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="491"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="OBA22623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="504" mass="57214" checksum="E8EC7D8AE2E320B0" modified="2016-10-05" version="1">MTDQNWKDSLNIPEKDTRVQTEDVLNTKGKSFEDLNLKRELLMGIFEAGFEKPSPIQEESIPMALAGRDILARAKNGTGKTASFVIPALQLVKSKLNKTQVLILVPTRELALQTSQVVRTLGKHLGINCMVTTGGTNLKDDIMRLNEPVHILVGTPGRVLDLAARSVADFSECPLFVMDEADKMLSREFKSVIEQILSFFPQKRQSLLFSATFPYVVKSFMDKHLTKPYEINLMDELTLRGISQFYAFVEEKQKLHCLNTLFSKLQINQSIIFCNSTNRVELLAKKITELGYSCYYSHAKMPQQARNKVFHEFRQGKVRNLVCSDLLTRGIDVQAVNVVINFDFPKSAETYLHRIGRSGRFGHLGLAINLMSWNDRYSLYQIEQELGTEIKPIPATIDKSLYVNENEESIPRPFKLEELPRGKDRRGGFEYQGQPQSQPPASQSPPQQHQPSQIPAQGQLGVQYPQYPQGVPPQFNYPNQYQVPPQYNGYPPQFGGQFPVQQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFT6</accession>
    <name>A0A1A0HFT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_75911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713199.1">
        <property type="nucleotide sequence ID" value="XM_018858445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFT6"/>
    <dbReference type="STRING" id="869754.A0A1A0HFT6"/>
    <dbReference type="GeneID" id="30031421"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534:SF49">
        <property type="entry name" value="LYSINE BIOSYNTHESIS REGULATORY PROTEIN LYS14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37534">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR PROTEIN UGA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="3">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="113"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA22718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="749" mass="84054" checksum="11B7568EFBDC22CE" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFV1</accession>
    <name>A0A1A0HFV1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_75920</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713214.1">
        <property type="nucleotide sequence ID" value="XM_018858447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFV1"/>
    <dbReference type="STRING" id="869754.A0A1A0HFV1"/>
    <dbReference type="GeneID" id="30031423"/>
    <dbReference type="OrthoDB" id="196103at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010291">
        <property type="entry name" value="Ion_channel_UNC-93"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051617">
        <property type="entry name" value="UNC-93-like_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294">
        <property type="entry name" value="ET TRANSLATION PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23294:SF59">
        <property type="entry name" value="UNC93-LIKE PROTEIN C922.05C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05978">
        <property type="entry name" value="UNC-93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="433"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="484" mass="53566" checksum="E6CE762FBD97D9DB" modified="2016-10-05" version="1">MVDSSSDVDHTEIVCHSWAEIEASGNWYDRRYVSWIPPFSHSTVQIVLMSFVIFLTPGCFNAITGIGNSGISDPTVADNANVALYSTFATVGAIGGVVCNTIGVRACLVFGGTGYIMYTLSLLVYHYKENAGFCIFAGAYLGVSASFTWAAQGSIIMSYALENKKAHAIMIFWVIFNFGAVIGSIIPLVQNLESGESNVNAGTYVAFLVLMGCGVLLACFLLPMDKVYKPDGSKVIAKKHPRLRDEIRGMIKVLVHEPKIYFMFPMFCALNFFYTYQFNEFNAVRFNVRTRSLNSLLYWFSQMVGAIVLGLMMDSPRFSRPTRARIGYAVLTVTSLAIWGGGLKFQIGVTRETDVVLIDFKDGLYIGPMFLYMFYGAYDAVFQSFIFWVLGTFSNNPKKVALYASFYKSLQSAFSAIAWRLDAIGVPYMSMFAASWGLVQGSLLIAAPLVLFMITETTDPDKDGIDEIMEASELRSVTSHPQKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFW2</accession>
    <name>A0A1A0HFW2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioesterase/thiol ester dehydrase-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_37396</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713372.1">
        <property type="nucleotide sequence ID" value="XM_018856870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFW2"/>
    <dbReference type="STRING" id="869754.A0A1A0HFW2"/>
    <dbReference type="GeneID" id="30029846"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="3">
        <location>
            <begin position="44"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="4">
        <location>
            <begin position="190"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="334" mass="39270" checksum="243E705B587D0E6C" modified="2016-10-05" version="1">MSSEDTFRSTKMCKMEEKFLLIQKTPTEFIGRYPLEPFREDARGTYGGEFVAQSLRAAWDSIEDREFDIHSLHSYFLKAGLKDSVMRYEVTTLSLGRSFSSKLVKCYQLHNDQLCFTLMASFTRNNNIQQRKLEFSKLPEKEMRHPRTKVPFEFLRSPHYTFHKYVDKLQSMPFMEHTNGNLSHIFVPETWSAPEDEMEKDPGARKFGLFFKVNDNVASAHDVEKAKMVDFAFASDSLYLSTVMRAVLPKLDYKKGNFFRVSLDHTVYYHDTNFDPTEWMYLDYKFERLSNDRVLVTSRYFTTDERLVAIALQEALCFFPLELIENNKLGSYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGE5</accession>
    <name>A0A1A0HGE5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cyclin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_29511</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713433.1">
        <property type="nucleotide sequence ID" value="XM_018855421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGE5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGE5"/>
    <dbReference type="GeneID" id="30028397"/>
    <dbReference type="OrthoDB" id="340962at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
        <property type="entry name" value="Cyclin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="178"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="427" mass="48125" checksum="1EB1D967C3B8406D" modified="2016-10-05" version="1">MQAPETLLSSGGSMSKNNPEQINSAAAHEPINGATSSPGPQPVAPTIDCADAGSTQNTVSPDFPVQSGNIIEKLSSGSISPAETGPKPEKKSVSGDDLYRRSSQYQTWSFTSEELYLIKKQTNEKGRATALQLFESSRSSLEAEKPEVFAAHGDKLTSEILELISFDEEQKYLHYFGQQIVQICAHFKMPTQVKATAISFFRRFYLVNSVMEYRPRNVLYTAVFLAAKLENYFISIETFCSRIPKTKPEDILGLEFIILQALKFTLLVHHAFRPVYGFFLDFQLLLLHPAPVMYDVNVDTIGQLYDKAKKWLNDHALLSDVSFLFTPPQIALAALYDSDKRITDRYLRLKFLHEPPEGKEVGQSSVPPHVKEHFDLILKTIKRCIKIAKEETTTSKEESTKIDEKCFFALNPLKLLKRRIKALPEQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGI7</accession>
    <name>A0A1A0HGI7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713597.1">
        <property type="nucleotide sequence ID" value="XM_018855453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGI7"/>
    <dbReference type="GeneID" id="30028429"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="86" mass="9550" checksum="1F016B090F470391" modified="2016-10-05" version="1">MILRIYKKSPAPAMHLFPPGLILLYVQFALPLLELTAKTHQGNINHMAFSSQNPRKHRPNSFAGSFEDPNGGCAKRPGSAQYAPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGJ5</accession>
    <name>A0A1A0HGJ5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_36402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713596.1">
        <property type="nucleotide sequence ID" value="XM_018856735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGJ5"/>
    <dbReference type="STRING" id="869754.A0A1A0HGJ5"/>
    <dbReference type="GeneID" id="30029711"/>
    <dbReference type="OrthoDB" id="4019734at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="344" mass="37835" checksum="44067373FE021A2A" modified="2016-10-05" version="1">MLPALRSWTLGKTASVRGYSTLGGPAGSSGFLSDLMKRIDTINMKSEKIAQLALRPTEHRQPSSRKAPAAADPPKKQKMPLAPKKYTRAPPRAKIVVKDHPLSSNMFKLMDEKNFRGGARDARPPGPRQGQNQAFRGEKPPFRGEKPAAYSQGRPQQRNMQPGDRRQGGNRPADGRRNPGAQKARNFVKPASIVPKKLKAAALKPTLSGDVFLHGRPAKLAVCTSSRVAAVAKECLLESHYPYKLPRSIIDNIDEGFSGNRFLLQKDWNLEVDAEHFSERIRKVVRGEADSLSVPKDATPAEQDTCRGLMSNGSLSMDDKRTIYDVVAGVKSVLDLLKNAAWNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGK6</accession>
    <name>A0A1A0HGK6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Telomere-associated protein Rif1 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_35961</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713493.1">
        <property type="nucleotide sequence ID" value="XM_018856676.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HGK6"/>
    <dbReference type="GeneID" id="30029652"/>
    <dbReference type="OrthoDB" id="4070686at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140445">
        <property type="term" value="C:chromosome, telomeric repeat region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022031">
        <property type="entry name" value="Rif1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22928:SF3">
        <property type="entry name" value="TELOMERE-ASSOCIATED PROTEIN RIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12231">
        <property type="entry name" value="Rif1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Telomere-associated protein Rif1 N-terminal" evidence="8">
        <location>
            <begin position="279"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="90"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1115"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1155"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1163"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1190"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1214"/>
            <end position="1282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1393" mass="156061" checksum="B80950CAF7B296EC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGL1</accession>
    <name>A0A1A0HGL1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SecY protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_9343</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713498.1">
        <property type="nucleotide sequence ID" value="XM_018859359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGL1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGL1"/>
    <dbReference type="GeneID" id="30032334"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071261">
        <property type="term" value="C:Ssh1 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="3">
        <location>
            <begin position="43"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA23017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="491" mass="52096" checksum="AF2EC5D479D98E37" modified="2016-10-05" version="1">MSRFRLLDLVKVVSPIVPEINYPLENVTIDEKVVFTIASALIFMLSQIPVFGLVRDAPLKMTDPFFSFRPLFAMEQGSLLELGLLPVLTSAFAWQLAAGLKLVRVKFAFGVDRELFQLAQKVTSLLLSAVFVVALIVSGYYDPVVRGASHESNAYGSYALIFAQVFGWNVVLTLMVEVLDKGYGFGSGVLCFLALNAATSLVRDVVGLELVSLTPDGVPQTYGVIAYLGKSLLSMELSTIKEAAVGIFIRSGYPSIGMVLIAVATGLAVIVLQNCRLELPVRSNRARGSANIYPIRLLYTGALPVVFAFTVVANVQILLHFLAAAVAPFYPLVASMLASRDASGKLVSGLAFYFSSPASLTASLLSPIRAVVYTGSIVVLASAFAIIWADISGSAPRDIAKQFKSQEIVISGKRDVSITKELSRTIPVAAVSGAVSLAALALVGELLGASGKTVSVTVGIVSAFSVLEDFMNELQQSGGSSQFVGSMAGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGS7</accession>
    <name>A0A1A0HGS7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_29864</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713860.1">
        <property type="nucleotide sequence ID" value="XM_018855500.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGS7"/>
    <dbReference type="STRING" id="869754.A0A1A0HGS7"/>
    <dbReference type="GeneID" id="30028476"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="5">
        <location>
            <begin position="28"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="404"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA23379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="494" mass="55379" checksum="2C1BBD0BEFB63DA6" modified="2016-10-05" version="1">MSVAVDAMAAAEQQITLEHQLREKVVNEEFKIWKKTVPLLYNSIHTHALKWPLLTVDFSPKYTISDDKKSLEVLFVTGSNTSQRDQDTVSLRTINLPSTMSPDFASFGTGENIIVSESASNSIDLGVKRLWSHAGEVNRAKFSPIGEKFVTFDNAGVVHLYDINQEMPVDFKFHKTEGYCLEWVSENQFLSGANDSQIALWNASSPAAPEKQFNTHTAVINDISFSKPAAVLFGSVADDFTTQIHDLRTTEDSPAIKIVEKHLQNAIQLHPEIGHLFATGGKDNLVNVYDARLTDRPIRQLFGHNDLIFGLRWDNIDPLQLYSWGLDKRVISWDLNYLTEEYAPPSTESQETKKRSKHTEDPCLQFIHAGHTNRVNDFAVHPSIPNLFASVGDDTLLEIYQPKTLREGDEDDEDEMAEEQDPEENEAVNGEADAASGTYEKKDADEDIDQIVDSPKLEEKTNFTADNGLHEQFGINSKPAAQEEHSNSDVDMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH19</accession>
    <name>A0A1A0HH19_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor MBP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_35231</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713955.1">
        <property type="nucleotide sequence ID" value="XM_018856583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH19"/>
    <dbReference type="STRING" id="869754.A0A1A0HH19"/>
    <dbReference type="GeneID" id="30029559"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000004">
        <property type="entry name" value="Transcription factor MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF15">
        <property type="entry name" value="TRANSCRIPTION FACTOR MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13606">
        <property type="entry name" value="Ank_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="7">
        <location>
            <begin position="8"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00073969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="887" mass="100717" checksum="DFDEFED94761150C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH70</accession>
    <name>A0A1A0HH70_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">2-oxoglutarate reductase</fullName>
            <ecNumber evidence="4">1.1.1.399</ecNumber>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_76376</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-2-hydroxyglutarate + NAD(+) = 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49612"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.399"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.399" evidence="4"/>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713826.1">
        <property type="nucleotide sequence ID" value="XM_018858519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH70"/>
    <dbReference type="STRING" id="869754.A0A1A0HH70"/>
    <dbReference type="GeneID" id="30031495"/>
    <dbReference type="OrthoDB" id="1621027at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061759">
        <property type="term" value="F:2-oxoglutarate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04901">
        <property type="entry name" value="ACT_3PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12176">
        <property type="entry name" value="PGDH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000036">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000041">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008759">
        <property type="entry name" value="PRK11790.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="395"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA23345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="465" mass="50608" checksum="55FF11D8B46C7153" modified="2016-10-05" version="1">MSAPQDIASSFKQALNLDSGSPNAVSTSPTQSYLSQFANSQKNSKALKPFSTGDIKILLLENVNITAIDLFKNQGYQVEFYKSSLPEDELIEKIKDVHAIGIRSKTKLTENVLRHAKNLAVVGCFCIGTNQVNLEYAAKAGIAVFNSPFSNSRSVAELVIAEIITLARQLGDRSIELHTGTWNKVSAKCWEIRGKTLGIVGYGHIGAQLSVLAEAMGMNVIYYDVMMIMALGNSKQVDSLDELLQKADFVTLHVPATPETKNLLSTPQFAAMKDGSYVINASRGTVIDIPALIQAMKSGKIAGAALDVYPHEPAKNGEGLFSNELNSFAEDLCSLRNVILTPHIGGSTEEAQSAIGVEVGTSLIKYINEGNTVGAVNFPEVALRDLDLDQENSVRVLYTHQNVPGVLKTVNNILSNHNIEKQFTDSRGDVAYLMADISDVDMSDIKSLYEQLEQTPYKIATRLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHB8</accession>
    <name>A0A1A0HHB8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Inner centromere protein ARK-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_9667</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the INCENP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713877.1">
        <property type="nucleotide sequence ID" value="XM_018859433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHB8"/>
    <dbReference type="STRING" id="869754.A0A1A0HHB8"/>
    <dbReference type="GeneID" id="30032408"/>
    <dbReference type="OrthoDB" id="6123at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005635">
        <property type="entry name" value="Inner_centromere_prot_ARK-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03941">
        <property type="entry name" value="INCENP_ARK-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inner centromere protein ARK-binding" evidence="8">
        <location>
            <begin position="843"/>
            <end position="894"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="80"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="441"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="768"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="588"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="798"/>
            <end position="812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA23396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="909" mass="99337" checksum="8EA1CC0B1A9DCCCD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHD9</accession>
    <name>A0A1A0HHD9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_37562</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily. YAT (TC 2.A.3.10) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713896.1">
        <property type="nucleotide sequence ID" value="XM_018856888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHD9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHD9"/>
    <dbReference type="GeneID" id="30029864"/>
    <dbReference type="OrthoDB" id="3900342at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000017">
        <property type="entry name" value="Amino acid permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004762">
        <property type="entry name" value="Amino_acid_permease_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00913">
        <property type="entry name" value="2A0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF1">
        <property type="entry name" value="GENERAL AMINO-ACID PERMEASE GAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="250"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="459"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="499"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="10">
        <location>
            <begin position="70"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="571" mass="62881" checksum="F4D4C5B6698341FC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHH2</accession>
    <name>A0A1A0HHH2_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_104089</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713808.1">
        <property type="nucleotide sequence ID" value="XM_018853764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHH2"/>
    <dbReference type="STRING" id="869754.A0A1A0HHH2"/>
    <dbReference type="GeneID" id="30026740"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015149">
        <property type="term" value="F:hexose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="389"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="18"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="492" mass="53103" checksum="17923B608B736E00" modified="2016-10-05" version="1">MRAMAAARDSLTVKLIVTVSTVCLGSLQFGYHMAEMNSPGAILSCRADVPGLNPYELSFFGRNGFSSCIPLTNEQVGLATSIFSIGGLVGSFYVGTLADSIGRKKTALLHNVFFFLGSMLNGFAGTFIALMMGRFIAGIGAGSALVITSLMINEIAPPSYKGFLGSMNQVSINIGILFAQFLALGWNNNNDWRYLLFMGSAIAAFNFGAIILYGEESPVWLFNKGMATQAFTTLHKLRGGEYAASRTEVNSWKTSSSSTAPEDGSLLNEEGVDTRESERGENGAISLKKYLTSSEYNNSKLVGTGILVLQQFCGINSIIFYGVSVLINIFPEAAVLINCLISIVNTLVTFASAPFVDKLGRKPLLKTSVCMMGVLAAVLGYGISRSNAIFSILGTFTYITFFAVGLGPIPFLLVGEVTQPRAKALAQSWGVTMNWLATFVVGFFFPIIKESRIGAGVYYIFSGMCGLAYYFISNYIPETKGCNTYEEVWSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHH4</accession>
    <name>A0A1A0HHH4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_9863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714095.1">
        <property type="nucleotide sequence ID" value="XM_018859475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHH4"/>
    <dbReference type="STRING" id="869754.A0A1A0HHH4"/>
    <dbReference type="GeneID" id="30032450"/>
    <dbReference type="OrthoDB" id="10254604at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020">
        <property type="entry name" value="FLAVIN REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15020:SF50">
        <property type="entry name" value="UPF0659 PROTEIN YMR090W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="222" mass="24406" checksum="FB064CE1F5F316C8" modified="2016-10-05" version="1">MSKVIVFGSHGKVGQNLIKLLALSDKYQATAVIRSPTQASTLRNLDEATANLNTSQLDMGSSSVDDLTKAIRGHNAVILTVGSAGKDLLKVDLDGVVKTFEASVQANVRRLILVSAVYAHDRVFGAATPLRDYYISKHYADRILKNEFRDALDFTILKPSRLTDGEGTGRIRILTGEQDVGLVDRKDVARAIFEILDEKSTFGKEYDFTSGDLDISDRRTWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHK0</accession>
    <name>A0A1A0HHK0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76623</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ING family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714120.1">
        <property type="nucleotide sequence ID" value="XM_018858562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHK0"/>
    <dbReference type="STRING" id="869754.A0A1A0HHK0"/>
    <dbReference type="GeneID" id="30031538"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF94">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G11940)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="9">
        <location>
            <begin position="195"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="206"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="7">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="265" mass="30291" checksum="2F71F9BF61495426" modified="2016-10-05" version="1">MASVVKSSSSVYSTYISTVDHLPCDIVRSLWVVQACNLAADSERQKLHDLLRHVRPSHVHQQPDRQGFLHAYQELRLKIQRWNREAVAELESLGIQMKTHGAMLRRGVGLLRVTAQAPQAPEPRASSELLKLQLDEHYRENPLASQVEALQERVLHGLRNVVIKHVPGRSSGVKIILKLPKARDRGAAPGEPERYCFCHQPSFGDMIACDFAKCKNGEWFHYKCVGLLNRAEVSRYKTQKWYCSAACQSDAEKHKPRKKRRRANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHP7</accession>
    <name>A0A1A0HHP7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_9640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713849.1">
        <property type="nucleotide sequence ID" value="XM_018859430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHP7"/>
    <dbReference type="STRING" id="869754.A0A1A0HHP7"/>
    <dbReference type="GeneID" id="30032405"/>
    <dbReference type="OrthoDB" id="410267at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576:SF166">
        <property type="entry name" value="ADR278WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21576">
        <property type="entry name" value="UNCHARACTERIZED NODULIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="316"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="418"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="452"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="259"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="530" mass="56825" checksum="DC8894115A1CCC2A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHR0</accession>
    <name>A0A1A0HHR0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_107435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714022.1">
        <property type="nucleotide sequence ID" value="XM_018853841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHR0"/>
    <dbReference type="GeneID" id="30026817"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8611" checksum="EC36FD9888160AF6" modified="2016-10-05" version="1">MLILPSCLRPEKCRGGPVNTYMPQSHLFIFNSPAGPKAGLSFEMVHMSSLTIQRRLALSLHTDDTLYKDLKKNYSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHV9</accession>
    <name>A0A1A0HHV9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714076.1">
        <property type="nucleotide sequence ID" value="XM_018855535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHV9"/>
    <dbReference type="STRING" id="869754.A0A1A0HHV9"/>
    <dbReference type="GeneID" id="30028511"/>
    <dbReference type="OrthoDB" id="4080041at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="847" mass="96222" checksum="5DD66A9136C20D30" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIC7</accession>
    <name>A0A1A0HIC7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pkr1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_36935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714074.1">
        <property type="nucleotide sequence ID" value="XM_018856802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIC7"/>
    <dbReference type="STRING" id="869754.A0A1A0HIC7"/>
    <dbReference type="GeneID" id="30029778"/>
    <dbReference type="OrthoDB" id="9626941at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013945">
        <property type="entry name" value="Pkr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28251:SF1">
        <property type="entry name" value="V-TYPE ATPASE ASSEMBLY FACTOR PKR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08636">
        <property type="entry name" value="Pkr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA23593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="116" mass="12782" checksum="ED85C2B0CDA214FE" modified="2016-10-05" version="1">MSFFVELWESIFTPGTSPALMKATHASFILLLLSLLFLIFYSGSIHFINLFVIASCLYAAVIWFVNELKNIKLKDNEELAKETGGLVSVPSEKKQVESGSTSGVKKPIGAPTKRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIK2</accession>
    <name>A0A1A0HIK2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_10049</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714312.1">
        <property type="nucleotide sequence ID" value="XM_018853674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIK2"/>
    <dbReference type="STRING" id="869754.A0A1A0HIK2"/>
    <dbReference type="GeneID" id="30026650"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060962">
        <property type="term" value="P:regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22701">
        <property type="entry name" value="FHA_FKH1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045178">
        <property type="entry name" value="Fhl1/FHA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21712:SF29">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN FHL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FHA" evidence="5">
        <location>
            <begin position="55"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Fork-head" evidence="6">
        <location>
            <begin position="439"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="3">
        <location>
            <begin position="439"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="265"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="392"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="754"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="288"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="408"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="762"/>
            <end position="786"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="821"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="848" mass="89138" checksum="EDC77F28AB1D209B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIL4</accession>
    <name>A0A1A0HIL4_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein kinase SCH9</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_17783</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714208.1">
        <property type="nucleotide sequence ID" value="XM_018854734.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIL4"/>
    <dbReference type="STRING" id="869754.A0A1A0HIL4"/>
    <dbReference type="GeneID" id="30027710"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904828">
        <property type="term" value="P:positive regulation of hydrogen sulfide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060963">
        <property type="term" value="P:positive regulation of ribosomal protein gene transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045945">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901494">
        <property type="term" value="P:regulation of cysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047484">
        <property type="term" value="P:regulation of response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090153">
        <property type="term" value="P:regulation of sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000116">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="54"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="235"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="15">
        <location>
            <begin position="497"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="586"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00074478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA23727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="632" mass="69562" checksum="D3B328014A036846" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIY8</accession>
    <name>A0A1A0HIY8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MYND-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_76883</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MUB1/samB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714447.1">
        <property type="nucleotide sequence ID" value="XM_018858603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIY8"/>
    <dbReference type="GeneID" id="30031579"/>
    <dbReference type="OrthoDB" id="5594178at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990304">
        <property type="term" value="C:MUB1-RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051664">
        <property type="entry name" value="MYND-type_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47442">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN MUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47442:SF1">
        <property type="entry name" value="MYND-TYPE ZINC FINGER PROTEIN MUB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="9">
        <location>
            <begin position="718"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="210"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="793"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA23966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="821" mass="92457" checksum="000F9CE10808C37A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HIZ0</accession>
    <name>A0A1A0HIZ0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin-like 1-activating enzyme E1A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_118232</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714605.1">
        <property type="nucleotide sequence ID" value="XM_018854103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HIZ0"/>
    <dbReference type="STRING" id="869754.A0A1A0HIZ0"/>
    <dbReference type="GeneID" id="30027079"/>
    <dbReference type="OrthoDB" id="1708823at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF162">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="9">
        <location>
            <begin position="15"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA24124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="345" mass="38260" checksum="79E3B5CAE3701E3C" modified="2016-10-05" version="1">MSTPEKLSADEIALYDRQIRLWGTETQLRLRSAKVLVIRLGGVGTETVKNLVLGGIHSIEILDDSVVKEEDFATQFFLPNDDSIIGQRKLPHVISSIQELNTRVHLLTSTNDVLEIGADYLRTFDMVIATELDKSELLRINELTRKLQIPLYVAGLHGMFGYILTDLIQHTSIKNLDKGNQSRTSGTAINGVKTITAVESVEKENKERVTVEDTYVPLAEIFTSTKLPSQLTRRQLKRLSGALPLVMSLFEIARPSNASEPVDQKHLGEVLKVVCERLGLPESTISAEYLSALASQAYTEFAPTAAIVGGVLAQDVIQYFGQKDSPINNCLIFDAMQSELPIYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ01</accession>
    <name>A0A1A0HJ01_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_37645</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714343.1">
        <property type="nucleotide sequence ID" value="XM_018856901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ01"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ01"/>
    <dbReference type="GeneID" id="30029877"/>
    <dbReference type="OrthoDB" id="10036721at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030174">
        <property type="term" value="P:regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000241">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054125">
        <property type="entry name" value="MCM5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21933">
        <property type="entry name" value="MCM5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="13">
        <location>
            <begin position="323"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA23862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="727" mass="81673" checksum="10A2D5B874B06874" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJ75</accession>
    <name>A0A1A0HJ75_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="6">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_36392</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714532.1">
        <property type="nucleotide sequence ID" value="XM_018856734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJ75"/>
    <dbReference type="STRING" id="869754.A0A1A0HJ75"/>
    <dbReference type="GeneID" id="30029710"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16489">
        <property type="entry name" value="mRING-CH-C4HC2H_ZNRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051878">
        <property type="entry name" value="ZNRF_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNRF1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46661:SF4">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0458">Lysosome</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0519">Myristate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="20">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="280"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA24051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="326" mass="35594" checksum="7AFB9764F22ED9CC" modified="2016-10-05" version="1">MPVWKADATASKCYLCKARFTLFNRRHHCRKCGQLVCATCSSKQVRYFANSYVLNSQNTPEKARSHVYYRTCDVCVDEIHMMRSALSPVPATETASVLQGHRDEPPAAVGPSADVHMIPGATSAANPLKTAPNRAQHASSDPPEFTGSNEPHDADSDADLCPVCGVDLGKQFLEESLGGPLASSAAYETYKEAHVSRCLVTYDFASNHLRLSSPPDGLHARNRMLVYNMPPIPEPSYETISDGSPNAGAVAAKTDGEIPGLVRSTATFSGTEKDDMDAECVICLEDLCPGDKVGRLECLCVFHYKCIKDWFNKKSYAECPVHYLHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJG1</accession>
    <name>A0A1A0HJG1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">t-SNARE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_34929</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714617.1">
        <property type="nucleotide sequence ID" value="XM_018856539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJG1"/>
    <dbReference type="STRING" id="869754.A0A1A0HJG1"/>
    <dbReference type="GeneID" id="30029515"/>
    <dbReference type="OrthoDB" id="10251371at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15845">
        <property type="entry name" value="SNARE_syntaxin16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006012">
        <property type="entry name" value="Syntaxin/epimorphin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF83">
        <property type="entry name" value="SYNTAXIN-16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00914">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="313"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="12">
        <location>
            <begin position="239"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="339"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA24136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="367" mass="41975" checksum="7F14587F33AC4C8D" modified="2016-10-05" version="1">MFRDRTNLFLSYRRTVPRLEAPGARRTVSLAEEEESLMSARRSMNGPRNLNSYRDDASRPNEIEMRPLAPSIFEISSLLDTNLAAIRQKTTELSLLYKKLLITRDSEKKPLEDGIDGLNYNITRMFEACYVLIKKFEFIQKNHVRLRLAYSADELSILENYKKTYALKIQDSLLVFRNLQNNYIKFLKDDDDDDDSHNLLLSSASQHIPLEEEDSRDIEDYSRQALRQAQQQIQQDPNSQLMASREREISKLAMGILEISTIFKEMENLVVDQGSMLDRIDYNIGRTAEDLRASDKELVRAQGHQKRTTKCKIIFLLCLVVLALFMVVMIRPSRKTTVVEKPSLGAGTPGQSGPQSRPTKQAGAPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJN6</accession>
    <name>A0A1A0HJN6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">WAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_30537</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714692.1">
        <property type="nucleotide sequence ID" value="XM_018855656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJN6"/>
    <dbReference type="STRING" id="869754.A0A1A0HJN6"/>
    <dbReference type="GeneID" id="30028632"/>
    <dbReference type="OrthoDB" id="332390at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018501">
        <property type="entry name" value="DDT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028942">
        <property type="entry name" value="WHIM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028941">
        <property type="entry name" value="WHIM2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013136">
        <property type="entry name" value="WSTF_Acf1_Cbp146"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32075:SF6">
        <property type="entry name" value="ISWI CHROMATIN-REMODELING COMPLEX SUBUNIT YPL216W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02791">
        <property type="entry name" value="DDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10537">
        <property type="entry name" value="WAC_Acf1_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15612">
        <property type="entry name" value="WHIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15613">
        <property type="entry name" value="WSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51136">
        <property type="entry name" value="WAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WAC" evidence="7">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="517"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="853"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1104"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1181"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="391"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="800"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="526"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="583"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="857"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1106"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1181"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA24211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1205" mass="137153" checksum="49ECBAAFA78368C8" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HJZ7</accession>
    <name>A0A1A0HJZ7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_30640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714826.1">
        <property type="nucleotide sequence ID" value="XM_018855675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HJZ7"/>
    <dbReference type="GeneID" id="30028651"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA24345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="60" mass="5996" checksum="4C5929CFAC8CBCC4" modified="2016-10-05" version="1">MQSKSIALYLAAASSVSATSSSNDFNHSNRTFSTAVADASQMVGVGVFGVAVVAGVAMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HK99</accession>
    <name>A0A1A0HK99_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phospholipase D/nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_30620</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714793.1">
        <property type="nucleotide sequence ID" value="XM_018855669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HK99"/>
    <dbReference type="STRING" id="869754.A0A1A0HK99"/>
    <dbReference type="GeneID" id="30028645"/>
    <dbReference type="OrthoDB" id="47785at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017005">
        <property type="term" value="F:3'-tyrosyl-DNA phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09196">
        <property type="entry name" value="PLDc_yTdp1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010347">
        <property type="entry name" value="Tdp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06087">
        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="11">
        <location>
            <position position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR610347-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA24312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="613" mass="69171" checksum="972F98223A1273EC" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKA3</accession>
    <name>A0A1A0HKA3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 repeat-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_77138</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714798.1">
        <property type="nucleotide sequence ID" value="XM_018858650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKA3"/>
    <dbReference type="STRING" id="869754.A0A1A0HKA3"/>
    <dbReference type="GeneID" id="30031626"/>
    <dbReference type="OrthoDB" id="361494at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000109">
        <property type="term" value="C:nucleotide-excision repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042238">
        <property type="entry name" value="Rad28/ERCC8/Ckn1/ATCSA-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46202:SF1">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="41"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="127"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="218"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="284"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA24317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="442" mass="48679" checksum="26F65005B81E71ED" modified="2016-10-05" version="1">MQSLLLERKTGRLRPSDFAVTVTESLYSTLRPVSPNVFPDNCHHNAAVNSLAVESSEHRYMLSGCGDSSIKIWDLRGERSPEAGRAEELREGSLAEAEQAFPRGLPPADSTAKPGGRLVPIAAIPRRAAHQFGVSAVEWWPRDTGMFLSASFDHTVKVWDTNEVAVVHTFDLASRVYALSVSAHRSNAYSSQAMIAVASDQPSVRLLDLRTASSAHTLAGHRGKTLAVGWHPHNPYMLALGGHDGEAKVWDIRRSKSCVARLDMTKTGVRSAAADRLNVGSSSVKAHLGPVNALAWDELGHVLYTAGNDDKIRVWDMVSSHAPPVNKLVNFGPLTRNKYLQTLPLVLGPRRETEVQHLHVASDNGDVLVFRALDGKNGPGGSSRTASIAYGHPYSATYYCGTMDGEIVPWREKWDRRQMRQIREMREIREIRETRQGTNEAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKD6</accession>
    <name>A0A1A0HKD6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">METBIDRAFT_133826</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Forms DNA polymerase zeta with REV7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714947.1">
        <property type="nucleotide sequence ID" value="XM_018854350.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HKD6"/>
    <dbReference type="GeneID" id="30027326"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016035">
        <property type="term" value="C:zeta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05778">
        <property type="entry name" value="DNA_polB_zeta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05534">
        <property type="entry name" value="POLBc_zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000007">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000002">
        <property type="entry name" value="DNA polymerase zeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056435">
        <property type="entry name" value="DPOD/Z_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030559">
        <property type="entry name" value="PolZ_Rev3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45812:SF1">
        <property type="entry name" value="DNA POLYMERASE ZETA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24055">
        <property type="entry name" value="POL3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase delta/zeta catalytic subunit N-terminal" evidence="24">
        <location>
            <begin position="95"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="23">
        <location>
            <begin position="740"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="22">
        <location>
            <begin position="984"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="479"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="523"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00066055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF24055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OBA24466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1580" mass="181756" checksum="E3EBF0FF6D50F5B5" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKJ8</accession>
    <name>A0A1A0HKJ8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714898.1">
        <property type="nucleotide sequence ID" value="XM_018856716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKJ8"/>
    <dbReference type="STRING" id="869754.A0A1A0HKJ8"/>
    <dbReference type="GeneID" id="30029692"/>
    <dbReference type="OrthoDB" id="2194681at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA24417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="50" mass="5545" checksum="65C80F6718266324" modified="2016-10-05" version="1" fragment="single">GDRISSNTLFYVTKTNNASLDNVWTDVFAKALEGKDLKELFLLIEPAQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HKX6</accession>
    <name>A0A1A0HKX6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="19">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_10682</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="19"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018715028.1">
        <property type="nucleotide sequence ID" value="XM_018853826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HKX6"/>
    <dbReference type="STRING" id="869754.A0A1A0HKX6"/>
    <dbReference type="GeneID" id="30026802"/>
    <dbReference type="OrthoDB" id="272481at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.40:FF:000001">
        <property type="entry name" value="DNA repair helicase (Rad3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.20:FF:000001">
        <property type="entry name" value="DNA repair helicase rad15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000135">
        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.20">
        <property type="entry name" value="Bacterial XPD DNA helicase, FeS cluster domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042493">
        <property type="entry name" value="XPD_DNA_FeS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 23" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="22">
        <location>
            <begin position="7"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="755"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="766"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="777"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA24547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="795" mass="91269" checksum="D66797719385220B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HL28</accession>
    <name>A0A1A0HL28_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cytosolic iron-sulfur protein assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CIA1</name>
        <name evidence="5" type="ORF">METBIDRAFT_10649</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat CIA1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA24513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018714994.1">
        <property type="nucleotide sequence ID" value="XM_018853819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HL28"/>
    <dbReference type="STRING" id="869754.A0A1A0HL28"/>
    <dbReference type="GeneID" id="30026795"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03037">
        <property type="entry name" value="ciao1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028608">
        <property type="entry name" value="CIAO1/Cia1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="186"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="306"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="374"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA24513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="388" mass="43534" checksum="8905B68EFFDC3EF9" modified="2016-10-05" version="1">MTKLLKTLKAHSDKVWSVSSHDKLPLLATASSDKTSKIYQLSLEHNFPLIGVLEETHKRSVRSVAFKPQLQSSENEFIDLPALSAGSFDSTISIWGIDEPEDLLENEQSDEEDGEESRLKREVDMLISPKNEWNLMALIEGHENEIKAVAWNRSGNYLASCSRDKTIWIWETDPETLEEFECISVLSDHEHDIKHIVWHPKYNILASSSYDDTVRLYQQDADDEDWSCVGILSGHEGTVWCSQFENPSSPTASKDKIRLASSSDDSTVRVWTCDKPGDSSRENSDLPSSIKHESDEMHWLLEQVLPQAHKYAIYSVSWSAKSGRIASAGADGQIVIYKESNGEWEIESSIKSAHGVYEINCVSWCNLSNGAEVLVSAGDDGNINIWEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ03</accession>
    <name>A0A1A0GZ03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_15023</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000011">
        <property type="protein sequence ID" value="OBA16958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709257.1">
        <property type="nucleotide sequence ID" value="XM_018854561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ03"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ03"/>
    <dbReference type="GeneID" id="30027537"/>
    <dbReference type="OrthoDB" id="2158884at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA16958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="352" mass="40127" checksum="C43A07C2B1AB8849" modified="2016-10-05" version="1" fragment="single">ILPIEYESFLKSYYMISRLGFGSFGCAILAKYRKSISDLLAPQPHKLGTLLEPLKYASSTKPHADGLVAIKVMKNQLKKPSDYLNVNEIKFILSVPSHSNLLQIFDLFIDSASGKLNIVMEPMDQNLYQFIQKHEGRAIAPWVIKSMLAQLLGAIKHIHSHGFFHRDVKPENILVSSAKHYYYKNIPPGKENDIYVLKLCDYGLSRHVTNTKDLTQYVSTRWYRAPEILLRRRSYSRPIDIWAFGSVAVELVNCRPLFAGLNETDQIWQILTKLGHPLFQGRCSEDLGGVWPEAVQLADKLGFIIPYIAGQTIQSILRTDFQDLAEAIQACFLWDPEARPTADTLSRHAYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0GZ86</accession>
    <name>A0A1A0GZ86_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_33840</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000010">
        <property type="protein sequence ID" value="OBA17005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709302.1">
        <property type="nucleotide sequence ID" value="XM_018856405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0GZ86"/>
    <dbReference type="STRING" id="869754.A0A1A0GZ86"/>
    <dbReference type="GeneID" id="30029381"/>
    <dbReference type="OrthoDB" id="6509975at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016274">
        <property type="entry name" value="Histidine_acid_Pase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963:SF18">
        <property type="entry name" value="ACID PHOSPHATASE PHO11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20963">
        <property type="entry name" value="MULTIPLE INOSITOL POLYPHOSPHATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000894">
        <property type="entry name" value="Acid_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00778">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="62"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="262"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="5">
        <location>
            <begin position="405"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000894-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA17005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="463" mass="52432" checksum="E1058E4A97587CD5" modified="2016-10-05" version="1">MVAISNLINGGLLLVDQLFYRDLASPEQASVESYNVVRFLGGSGPYVQHPGFGISTDIPDQCSLKQVHLMSRHGERFPSLSYGKRFEAIMDKVKLYNGTFRGELEFFNDYTYFVDNENNYETETTSINSEGTFSGTTDAMKHGLSFRAKYNSLFNASAETLKVFTANSARVHQTSNYFARGFMGDEYSDEAVEYYILDEDASMGANSMTPSYGCLNYQWDINEDIVGQYNESYLDGARKRLMKDNEKFNLTTSDVKNMFQWCAYEINVRGSSPVCDLFTNEEYVRYSYFQDLDNYYANSHGNNLSATVSSAWLNATRNFLMEKSPAYRVLLSFTHDNEIETFHAALGLFEPDEDLPTDHIPFPIPYSHIQVTPQGARTITEKFSCGDATYVRYVVNDAVIPIKTCQDGPGFSCELSDFEKYIDLRLEGKSYGTQCGINSTVPSVVSFLWDYAEKNYTTPDGDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H201</accession>
    <name>A0A1A0H201_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_227659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709454.1">
        <property type="nucleotide sequence ID" value="XM_018855191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H201"/>
    <dbReference type="STRING" id="869754.A0A1A0H201"/>
    <dbReference type="GeneID" id="30028167"/>
    <dbReference type="OrthoDB" id="5296287at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015355">
        <property type="term" value="F:secondary active monocarboxylate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035879">
        <property type="term" value="P:plasma membrane lactate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17316">
        <property type="entry name" value="MFS_SV2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23508:SF10">
        <property type="entry name" value="CARBOXYLIC ACID TRANSPORTER PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="458"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="75"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA18059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="524" mass="58090" checksum="058DA4518EFFE37A" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H239</accession>
    <name>A0A1A0H239_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_226803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000009">
        <property type="protein sequence ID" value="OBA18020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709415.1">
        <property type="nucleotide sequence ID" value="XM_018855185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H239"/>
    <dbReference type="GeneID" id="30028161"/>
    <dbReference type="OrthoDB" id="4093584at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA18020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="100" mass="11136" checksum="46EB3482EAEBFDEF" modified="2016-10-05" version="1">MSSESTKKTIPALIGLDDDSLKDLKFCVASFCAIVIIIFNYAYIMRQWVLDPELPMSTLAFYLATLICTMIVSIGFLAKVVHPRIYCTVDGPAIAEKKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Q2</accession>
    <name>A0A1A0H4Q2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">METBIDRAFT_47197</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Vacuole membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709551.1">
        <property type="nucleotide sequence ID" value="XM_018858134.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0H4Q2"/>
    <dbReference type="GeneID" id="30031110"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006995">
        <property type="term" value="P:cellular response to nitrogen starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016242">
        <property type="term" value="P:negative regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905356">
        <property type="term" value="P:regulation of snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05169">
        <property type="entry name" value="PIKKc_TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000020">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.150:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000371">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000006">
        <property type="entry name" value="Serine/threonine-protein kinase TOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.150">
        <property type="entry name" value="FKBP12-rapamycin binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009076">
        <property type="entry name" value="FRB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036738">
        <property type="entry name" value="FRB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024585">
        <property type="entry name" value="mTOR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026683">
        <property type="entry name" value="TOR_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08771">
        <property type="entry name" value="FRB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11865">
        <property type="entry name" value="mTOR_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01346">
        <property type="entry name" value="DUF3385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01345">
        <property type="entry name" value="Rapamycin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47212">
        <property type="entry name" value="FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0926">Vacuole</keyword>
    <feature type="repeat" description="HEAT" evidence="15">
        <location>
            <begin position="801"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="FAT" evidence="17">
        <location>
            <begin position="1342"/>
            <end position="1912"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="16">
        <location>
            <begin position="2086"/>
            <end position="2405"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="18">
        <location>
            <begin position="2431"/>
            <end position="2463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="OBA19016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2463" mass="278829" checksum="52BDE4668FCA4367" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4R0</accession>
    <name>A0A1A0H4R0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_47389</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709599.1">
        <property type="nucleotide sequence ID" value="XM_018858161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4R0"/>
    <dbReference type="STRING" id="869754.A0A1A0H4R0"/>
    <dbReference type="GeneID" id="30031137"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050797">
        <property type="entry name" value="Carb_Metab_Trans_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31668">
        <property type="entry name" value="GLUCOSE TRANSPORT TRANSCRIPTION REGULATOR RGT1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="819"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="916" mass="104452" checksum="FAD23990EF22C41C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4S8</accession>
    <name>A0A1A0H4S8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATPase expression protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_224688</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709576.1">
        <property type="nucleotide sequence ID" value="XM_018855164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4S8"/>
    <dbReference type="GeneID" id="30028140"/>
    <dbReference type="OrthoDB" id="4094059at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="47977" checksum="00C867B6D9E356EC" modified="2016-10-05" version="1">MVQSTRLLVRVYKNFARKFGHSVISAKDPVSFYPTASFLDNAAHEQRMKENEAAVPTRPSLQSVKHIYRDMVLPQDVCDLLWEPKTEDYVITSLAEDCALNGDSLHKIVSRSPVSETKLMFANIAISWLDKFKAQTLQLSELTGLEEQLLYNKPLQIISSDYTEDCLSSAENLKEFCDLLLKKGYSLESITQFILSSHTTLAQYKYILGYLCENMYVLNPVSISEFTGYIITDAYKRHTSNEKELLGYSEAFDCFLRERLLQVYPSSLRAVGAITLDKLAYLSSVSENFQTAIMTLTDLIQQHKTAPSKSTWGTFFAAYSKYAVEQDLLKSQILAQISALKPVIFHHRLTAHNFRFLLSKVVDNSHDLSHLVQLVQTSSPDILVECGGQVIKKLQQISNASNASSIVKAVQATQLSKALEAAGFQPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4V9</accession>
    <name>A0A1A0H4V9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribosomal RNA-processing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_47480</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709649.1">
        <property type="nucleotide sequence ID" value="XM_018858171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4V9"/>
    <dbReference type="STRING" id="869754.A0A1A0H4V9"/>
    <dbReference type="GeneID" id="30031147"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043628">
        <property type="term" value="P:regulatory ncRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd09862">
        <property type="entry name" value="PIN_Rrp44-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000309">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000010">
        <property type="entry name" value="Exosome complex exonuclease DIS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033770">
        <property type="entry name" value="RRP44_S1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17215">
        <property type="entry name" value="Rrp44_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="16">
        <location>
            <begin position="79"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="RNB" evidence="17">
        <location>
            <begin position="509"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA19114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="978" mass="111040" checksum="6BE391F48E9C6926" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H4Z7</accession>
    <name>A0A1A0H4Z7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">VLRF1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_33625</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The VLRF1 domain mediates binding to the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ANKZF1/VMS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709533.1">
        <property type="nucleotide sequence ID" value="XM_018856356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H4Z7"/>
    <dbReference type="STRING" id="869754.A0A1A0H4Z7"/>
    <dbReference type="GeneID" id="30029332"/>
    <dbReference type="OrthoDB" id="429841at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036266">
        <property type="term" value="C:Cdc48p-Npl4p-Vms1p AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072671">
        <property type="term" value="P:mitochondria-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047139">
        <property type="entry name" value="ANKZ1/VMS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041175">
        <property type="entry name" value="VLRF1/Vms1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036">
        <property type="entry name" value="ANKYRIN REPEAT AND ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16036:SF2">
        <property type="entry name" value="TRNA ENDONUCLEASE ANKZF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18826">
        <property type="entry name" value="bVLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52044">
        <property type="entry name" value="VLRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VLRF1" evidence="12">
        <location>
            <begin position="208"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="385"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA18998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="607" mass="68312" checksum="B93919B69B181B68" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H501</accession>
    <name>A0A1A0H501_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TPR-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_47287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA18987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709522.1">
        <property type="nucleotide sequence ID" value="XM_018858144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H501"/>
    <dbReference type="STRING" id="869754.A0A1A0H501"/>
    <dbReference type="GeneID" id="30031120"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291664" evidence="3">
        <location>
            <begin position="21"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA18987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="929" mass="104325" checksum="19439CEBB8DF8163" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H512</accession>
    <name>A0A1A0H512_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Condensin complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">METBIDRAFT_61256</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709548.1">
        <property type="nucleotide sequence ID" value="XM_018858316.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H512"/>
    <dbReference type="STRING" id="869754.A0A1A0H512"/>
    <dbReference type="GeneID" id="30031292"/>
    <dbReference type="OrthoDB" id="436262at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043007">
        <property type="term" value="P:maintenance of rDNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903342">
        <property type="term" value="P:negative regulation of meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044804">
        <property type="term" value="P:nucleophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070550">
        <property type="term" value="P:rDNA chromatin condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030466">
        <property type="term" value="P:silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007130">
        <property type="term" value="P:synaptonemal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070058">
        <property type="term" value="P:tRNA gene clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000272">
        <property type="entry name" value="Condensin complex subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024324">
        <property type="entry name" value="Condensin_cplx_su1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12922">
        <property type="entry name" value="Cnd1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="6 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 N-terminal" evidence="13">
        <location>
            <begin position="67"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="12">
        <location>
            <begin position="940"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="456"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="459"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR017127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="OBA19013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1124" mass="126399" checksum="2AA2F8D1C0E1A1C0" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H524</accession>
    <name>A0A1A0H524_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_33669</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709585.1">
        <property type="nucleotide sequence ID" value="XM_018856373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H524"/>
    <dbReference type="GeneID" id="30029349"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="79" mass="9485" checksum="BB935F8CB162D669" modified="2016-10-05" version="1" fragment="single">MDKAIRSFVHPSLYLRIICPAQNPPERHGRFSMPSLHRTNLQQRRRHRCFPKPYLQQLRNIQILVARAKIYQAHELDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H565</accession>
    <name>A0A1A0H565_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_80015</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709598.1">
        <property type="nucleotide sequence ID" value="XM_018859159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H565"/>
    <dbReference type="STRING" id="869754.A0A1A0H565"/>
    <dbReference type="GeneID" id="30032135"/>
    <dbReference type="OrthoDB" id="38125at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016768">
        <property type="term" value="F:spermine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006597">
        <property type="term" value="P:spermine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="18"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="307" mass="34993" checksum="8DEBFDD00DF9F2BC" modified="2016-10-05" version="1">MIDLANFSHPSISKINEKYWFHETSDEAFRGQSFGLEVVKILDHSTSPYQDILVFESATFGNVLILNGIIQCTERDEFAYQELITHVPIMSHPKPSKVLVIGGGDCGVVRELVKHIGKGVDHVTMVEIDKQVIDLSIKYLPEMAKFHNHPNVNILLQDGFKYLMDLKDVDETEKFDVIITDSSDPEGPAEEFFQVNYYNLLNNALKKNGVVVMQSSENIWLNLPYLSGLLEKASQVFSNIEYCQCYMPSYTSGQLGLIVATQDDSKNLQVPLRVFEDERELFKYYNSRVHQASFVLPTWADKALNRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H599</accession>
    <name>A0A1A0H599_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DUF862-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_47026</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709743.1">
        <property type="nucleotide sequence ID" value="XM_018858115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H599"/>
    <dbReference type="STRING" id="869754.A0A1A0H599"/>
    <dbReference type="GeneID" id="30031091"/>
    <dbReference type="OrthoDB" id="21221at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070646">
        <property type="term" value="P:protein modification by small protein removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF7">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="5"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="164" mass="18165" checksum="7F98C5A86D8D0EC5" modified="2016-10-05" version="1">MDEEFPVKVYVYDLSHGLAAVYSPIVLGSKIDAIYHTSAVIYGQEFYIDQGIQVCTKPGTTKYGTPIEVLDMGTTLIDQDTFHDFLQDLRDHDEGKYNAPAYDLFDNNCNHFTDVLLEFSVGRNLEDRILKLPQQVLANPNGQLLRQMLGGGSGGASGNAHFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5B7</accession>
    <name>A0A1A0H5B7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein mL50</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_47516</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL50 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000008">
        <property type="protein sequence ID" value="OBA19102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709637.1">
        <property type="nucleotide sequence ID" value="XM_018858181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5B7"/>
    <dbReference type="GeneID" id="30031157"/>
    <dbReference type="OrthoDB" id="3980895at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018305">
        <property type="entry name" value="Ribosomal_m50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10501">
        <property type="entry name" value="Ribosomal_L50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="222" mass="25593" checksum="5AC43E386F546FA5" modified="2016-10-05" version="1">MISTRFLGRSIGQRSFVTSPRSFFLKNIFGSKESKTKELIEKQDEYAVDPNSKILVLNEENSPSHKAFDPESDIPGFKISQWKEKLVLARDIEASVTKEMVADAINKAYQESHGSEISAESYATTSLGDLQKRFEFVKIVQQKLGFDIKDHTISRSHDVEYLYEALQKQIAHRWSSERNPNAIVLRPEDFKDQPNVYLNIEFSEKEQAEIFQDLKKQALENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H5H1</accession>
    <name>A0A1A0H5H1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_33359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709684.1">
        <property type="nucleotide sequence ID" value="XM_018856273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H5H1"/>
    <dbReference type="STRING" id="869754.A0A1A0H5H1"/>
    <dbReference type="GeneID" id="30029249"/>
    <dbReference type="OrthoDB" id="444325at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061632">
        <property type="term" value="F:RNA lariat debranching enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040194">
        <property type="entry name" value="Cwf19-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006768">
        <property type="entry name" value="Cwf19-like_C_dom-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006767">
        <property type="entry name" value="Cwf19-like_C_dom-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072">
        <property type="entry name" value="CWF19, CELL CYCLE CONTROL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12072:SF4">
        <property type="entry name" value="CWF19-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04677">
        <property type="entry name" value="CwfJ_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04676">
        <property type="entry name" value="CwfJ_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cwf19-like C-terminal" evidence="2">
        <location>
            <begin position="244"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Cwf19-like protein C-terminal" evidence="1">
        <location>
            <begin position="407"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="478" mass="54225" checksum="1C8677359ADD9C5E" modified="2016-10-05" version="1">MKSKFLVLYPDPAKLDAIIKKANVQHAKNGPFLAIVLLGCCLPAESPVTSPDAPTYFFGKSDEEGSFVDVSPNFTCVTSRNALLKLDSGVTLAFLNETLALANETDISIKADILFSYNWPYCIAADQRLTLVGDRAVDVVVRQLQPRYHFAAGSEMGKAHEHPVVAWSPKRSYRFVSLSREGTGSKWFYAFGIETGDDEVQMPSANPFLEDLKRPIEEENGGPATKQLRLDLRDGNDEPVKTRTKTVAPSECFFCLSNPRLESHMIVSIGKFSYLTVAKGPLTMPTRSLNFSGHAILIPIDHTPLLPDLKDVRQEIEQYMQSLADAFAKNGYDVAFYEISRPENVHFHIQMVPVPRGLIGKTFQRALDEKSRINNESFEHHSKLHFKKCLSEDPELQELENADRFIKFTIFSEKSKTMYITSLEEGKGLDLQFPRRVLAFLLRLPKRIKWDRCRQSMSQETIECQKFKLFYEGFDFTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H603</accession>
    <name>A0A1A0H603_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">QRI7</name>
        <name evidence="9" type="ORF">METBIDRAFT_222174</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="7">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000007">
        <property type="protein sequence ID" value="OBA19385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709917.1">
        <property type="nucleotide sequence ID" value="XM_018855151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H603"/>
    <dbReference type="STRING" id="869754.A0A1A0H603"/>
    <dbReference type="GeneID" id="30028127"/>
    <dbReference type="OrthoDB" id="10259622at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072670">
        <property type="term" value="P:mitochondrial tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017860">
        <property type="entry name" value="Peptidase_M22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01016">
        <property type="entry name" value="GLYCOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="8">
        <location>
            <begin position="58"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA19385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="426" mass="47516" checksum="D09960E43348A7E6" modified="2016-10-05" version="1">MQPTRLASRFVNFRIPHRCYKVLAIESSCDDSCVALLEKHTPNSYPVTVDEQKATLNSAKTGGIIPTAAHEFHQQNIALLVERFCGKHNISASNPPDLICVTRGPGMVGLLSAGLQFAKALAVAWNRPLIGVHHMLGHLLTAKLPLKTSSPDDLRYPFLSLLCSGGHTMLVLLKSLTDHEIIINTLDIAAGDSLDKCARELGFSGNMLGPELEKFVENIDEDKKNRFAQIKTHDANNEFGFRLKMPMRNTKRSKIPEKIEFGFASFLSTITSYKKDGLMTEESREFVAYKLQEVIFDHIVNRIQVAFMKHNLSEDNKFDEGILEAVNDFVCSGGVAANKVLRSKLFNDLDAGHTLKFHFPDLALCTDNATMIGNAGIEIFENLKKRSSLSILPIRKWPMNELLDVDGWLDVPENEYNSIVGFSNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6B7</accession>
    <name>A0A1A0H6B7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_45895</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a key role in the late stages of endoplasmic reticulum to Golgi traffic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit TRAPP (transport protein particle) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018709986.1">
        <property type="nucleotide sequence ID" value="XM_018857972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6B7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6B7"/>
    <dbReference type="GeneID" id="30030948"/>
    <dbReference type="OrthoDB" id="10254842at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1380.20:FF:000002">
        <property type="entry name" value="Trafficking protein particle complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017479">
        <property type="entry name" value="TRAPP_I_complex_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR017479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="247" mass="27669" checksum="8ECDA597480B5670" modified="2016-10-05" version="1">MIDKPILPSVESIGALTIHDVSKTGFGYNPSIGPNSNAITAETSSILLNKRVVNLTPTLHQSIYDRNVNNKKISEISLLTLGFLFCEIVSWAHGKSKDVLDLESRLNGLGYQIGQRYLELIKLREGIKYSKREIRIIEILQFIHGPFWKAVFGKPANELEKSQDVHDEYMIIDNMPPVSKFISIPKEYGDLNCSAFVAGIIEGALDSSGFHCTVVAHSAPAEETPLRTVFLVKFDESLFTREEIRFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6E0</accession>
    <name>A0A1A0H6E0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Pkinase-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_46191</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710000.1">
        <property type="nucleotide sequence ID" value="XM_018858002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6E0"/>
    <dbReference type="STRING" id="869754.A0A1A0H6E0"/>
    <dbReference type="GeneID" id="30030978"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030437">
        <property type="term" value="P:ascospore formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030447">
        <property type="term" value="P:filamentous growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031578">
        <property type="term" value="P:mitotic spindle orientation checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14137">
        <property type="entry name" value="STKc_GSK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039192">
        <property type="entry name" value="STKc_GSK3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF4">
        <property type="entry name" value="PROTEIN KINASE MCK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA19472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="374" mass="43158" checksum="7B32DEA9D4536709" modified="2016-10-05" version="1">MDTQEYVLDEVVDNETSQSLPMFVKEYKEIGKGAFGTVVEALLAHLDENGQPRPARLGPFAIKRVLAQTEYKCRELEILRAVNHPNVVCLKYFFDKRSRTDGKMYQNLVMECLPLNLQSEIKYYRHLKYTIPYPHMKAYTFQLARAMLYLHGMNISHRDIKPSNILIDPSTVRLKICDFGSAKQLEPNQPSVSYICSRFYRAPELIVGCTVYTTKIDIWGLGCVIAEMFLGKPIFQGVSSELQLKEVSKLLGPPPKTFFFNSNPQYRGNMFSTKLFTGTIQERFERIFSNSPPDVIDLLLKVLVYDPEARASAKSIMLHPFFDELKQESFKVYPRGAAEPIKLKLYDLSEYELNLMGPLRDEFLRPLQRTNEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6P3</accession>
    <name>A0A1A0H6P3_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_33330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710224.1">
        <property type="nucleotide sequence ID" value="XM_018856270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6P3"/>
    <dbReference type="STRING" id="869754.A0A1A0H6P3"/>
    <dbReference type="GeneID" id="30029246"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="248"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="292"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="498" mass="50176" checksum="CCC573886F0F1501" modified="2016-10-05" version="1" fragment="single">GSFSNTGDMYFGISGAPLVGTPFIVTSVTSWTNSGMMVFRRASGGSALLVIEQVLGGDGLSRITNDGSICLYNTFWLQTTSIDGSGCITVGSGAELDLQLAVGTLLFSVAETQTIYLESSDSVLSILGLSVSLIPDNTIKVAGFGNGNTIHLDILFLSYSYSSTTGILTLSLTFLLEVDIDIGTGYDESLFSTSSTLISRSISYSGPAPNSIPAVCSCVSSFPEVTTTAIASSSTFIASTDRSLGTITASSETSAPASSAAPASSEAPVSSEGPTSSAAPASSEDPVSSEGPTSSTTPAFSEAPASSAAPASSEAPAFSDTGSSFVGTTYTTTFVSTKSDGSVETDSGVVVVTTDSVGSATTASDLTRDVSEVGTGKPVDVSSTFITSRNSKVTDSCDGKSTCTWTSTDGAASASAGENSKTGVYSTLTTVTNGPSASDVTKLTVTLPSCIDCDTITTGGEPTSSITALVSSLSNSMSSITISASVNINHHGESVYCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6P4</accession>
    <name>A0A1A0H6P4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD40 repeat-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_45663</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat WDR6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710158.1">
        <property type="nucleotide sequence ID" value="XM_018857942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6P4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6P4"/>
    <dbReference type="GeneID" id="30030918"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002130">
        <property type="term" value="P:wobble position ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051973">
        <property type="entry name" value="tRNA_Anticodon_Mtase-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344">
        <property type="entry name" value="WD REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14344:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101908">
        <property type="entry name" value="Putative isomerase YbhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="191"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA19630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1014" mass="113400" checksum="73B47B11F4652E76" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6R7</accession>
    <name>A0A1A0H6R7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">25S rRNA (uridine-N(3))-methyltransferase BMT5-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_79647</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710178.1">
        <property type="nucleotide sequence ID" value="XM_018859104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6R7"/>
    <dbReference type="STRING" id="869754.A0A1A0H6R7"/>
    <dbReference type="GeneID" id="30032080"/>
    <dbReference type="OrthoDB" id="273345at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070042">
        <property type="term" value="F:rRNA (uridine-N3-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019446">
        <property type="entry name" value="BMT5-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF26">
        <property type="entry name" value="FERREDOXIN-FOLD ANTICODON-BINDING DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10354">
        <property type="entry name" value="BMT5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="25S rRNA (uridine-N(3))-methyltransferase BMT5-like" evidence="2">
        <location>
            <begin position="79"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA19650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="324" mass="36709" checksum="E9D6A61E9DB9799A" modified="2016-10-05" version="1">MGRKLKGRQFQAKGLKGALQVHIAQEKVKQDTQRALEAQSQTQKDKAKSVKSGGAKKRQPVHVVQKKAFIPFSPNETLLLVGEGDFSFACALVRQDLILPENLIATGFDSETEMQTKYPEAMENVKFLKESGVSVLHEVDATNLMQSLKLKSLGKNQRVRIFENGKRLNYVMFNFPHNGRGIKDVDRNIREHQKLVLGYFKSCIALFDVVNKRSNSAASGYLSEEDSTAEKIVMSLFEGEPYISWGVKALSRSVGYRVERSGAFDWPFFAGYHHRRTNSMKDTTKPASERDARIYLFDKALSKEEFEKLTGKKSKKRDDDSDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6S8</accession>
    <name>A0A1A0H6S8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_46219</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710188.1">
        <property type="nucleotide sequence ID" value="XM_018858010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6S8"/>
    <dbReference type="STRING" id="869754.A0A1A0H6S8"/>
    <dbReference type="GeneID" id="30030986"/>
    <dbReference type="OrthoDB" id="28455at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="201" mass="22728" checksum="23412EBD6AC9DB69" modified="2016-10-05" version="1">MSEPNFKPVTVTKPIPNLFDLGNLATFDPNPLDNAKLNDPEQKEAYLASVTRDNLQLLINQVLSLPIKTTTDTHGSSTGQDSTMTLIQLPEPTTALPREKSIPKDKPKTKWEKFAEIKGIKAKAKDGKMVYDEATGEWVPKWGYKGKNKDLDDQWLVEIDEKSKGTPNELIDPRSLARAERKKLVKKNELQHKRNVKNLQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6T6</accession>
    <name>A0A1A0H6T6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Patatin-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_45248</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710334.1">
        <property type="nucleotide sequence ID" value="XM_018857899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6T6"/>
    <dbReference type="STRING" id="869754.A0A1A0H6T6"/>
    <dbReference type="GeneID" id="30030875"/>
    <dbReference type="OrthoDB" id="10049244at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006642">
        <property type="term" value="P:triglyceride mobilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd07230">
        <property type="entry name" value="Pat_TGL4-5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF10">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3 4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="5">
        <location>
            <begin position="199"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="4">
        <location>
            <begin position="230"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="390"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA19809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="560" mass="63773" checksum="5B5D5CEA80A031BF" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6V4</accession>
    <name>A0A1A0H6V4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_32833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710286.1">
        <property type="nucleotide sequence ID" value="XM_018856138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6V4"/>
    <dbReference type="STRING" id="869754.A0A1A0H6V4"/>
    <dbReference type="GeneID" id="30029114"/>
    <dbReference type="OrthoDB" id="10258445at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA19761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="77" mass="8555" checksum="5DA5AD2D045E3FAE" modified="2016-10-05" version="1">MDPRVRQLFKQLVYMAKEYPAESGGYSRLVGQAKRAFRGTEIGHADDMHRALSKGEYIVKGTYEEPTPGGADRNPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H6Y9</accession>
    <name>A0A1A0H6Y9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH-ubiquinone oxidoreductase 49 kDa subunit mitochondrial</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_33306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000006">
        <property type="protein sequence ID" value="OBA19672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710200.1">
        <property type="nucleotide sequence ID" value="XM_018856265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H6Y9"/>
    <dbReference type="STRING" id="869754.A0A1A0H6Y9"/>
    <dbReference type="GeneID" id="30029241"/>
    <dbReference type="OrthoDB" id="1009at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.645.10:FF:000005">
        <property type="entry name" value="NADH-quinone oxidoreductase subunit D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01962">
        <property type="entry name" value="NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004739">
        <property type="entry name" value="PRK06075.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 49 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="6">
        <location>
            <begin position="183"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="453" mass="50991" checksum="C904D9E50AA596E6" modified="2016-10-05" version="1">MAPRLRAGAAPDPVKPYINAMGNMPSRVSEEGTTLSKTTFTEVLDEVDMTWGRDDDPKHVDKQNTKIRHFTINFGPQHPAAHGVLRLILELHGEEIVRSDPHVGLLHRGTEKLIESKTYMQALPYFDRLDYVSMMTNELVFALAVEKLLNVEVPLRGKYIRTLFGEITRVLNHCMSVLSHIMDVGGLTPFLWGFEEREKLMEFYERVSGARLHSAYVRPGGVSQDLPAGLLDDIYMWATQFGDRIDEVEELCTDNRIWKARTQDVGVVTADDALNYALSGVMLRGSGVPYDIRRAQPYDAYHLVDFDVAVGYNGDCYDRYLCRVAEFRQSLRIIEQCINDMPEGPVKVEDFKISPPPRSLMKEDMEALIHHFLLFTKGYAVPAGETYTAIEAPKGEMAVYVVSDGSERPYRCKIRAPGFAHLGAFDHVSRGNLLADAVAIIGTMDLVFGEVDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H734</accession>
    <name>A0A1A0H734_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DnaJ-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_45100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710371.1">
        <property type="nucleotide sequence ID" value="XM_018857877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H734"/>
    <dbReference type="STRING" id="869754.A0A1A0H734"/>
    <dbReference type="GeneID" id="30030853"/>
    <dbReference type="OrthoDB" id="442087at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="4">
        <location>
            <begin position="4"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="232"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="155"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="301"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="365"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA19846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="608" mass="69967" checksum="55E25EF27A7520E2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H7D7</accession>
    <name>A0A1A0H7D7_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA mismatch repair protein MutL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_72610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000005">
        <property type="protein sequence ID" value="OBA19890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710415.1">
        <property type="nucleotide sequence ID" value="XM_018858404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H7D7"/>
    <dbReference type="STRING" id="869754.A0A1A0H7D7"/>
    <dbReference type="GeneID" id="30031380"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032139">
        <property type="term" value="F:dinucleotide insertion or deletion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000404">
        <property type="term" value="F:heteroduplex DNA loop binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000710">
        <property type="term" value="P:meiotic mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03484">
        <property type="entry name" value="MutL_Trans_hPMS_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000003">
        <property type="entry name" value="DNA mismatch repair endonuclease MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="219"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="5">
        <location>
            <begin position="675"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="405"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="556"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="426"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="490"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="557"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA19890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="885" mass="97635" checksum="500FC8111F67CE56" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H868</accession>
    <name>A0A1A0H868_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NAD(P)-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43992</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710738.1">
        <property type="nucleotide sequence ID" value="XM_018857731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H868"/>
    <dbReference type="STRING" id="869754.A0A1A0H868"/>
    <dbReference type="GeneID" id="30030707"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="336" mass="37504" checksum="D7BEF9BEDEA1C60A" modified="2016-10-05" version="1">MVLNANFYKPESAPYFNPAEERKTAIVTGGNSGIGWYTVLHLYLHGYIVYMAGRNAEKVAHAVNEIHTEAVSRFGAYTEEEQKRKPLGELHSLHLDCCDLKSVEICAEEFLTKESHLDLLINNAGLMAVPFEMTKDGYEIQYQVNLVAPILFTEKLLPALEKTTTQASVPRVITLSSMGHNMSIKYWDPANPINKFPSALYSWVRYGNAKRGAIEYMRKFAQVHPEILAFSVHPGVITDTRLYDWWTSMPTLGPLFKLGFQGCGKVIGVSCEEGALATLRAALDPNLSAQESGSYFVTGGVIEAPSKVAQKQENIDTTWSYNIRLLTEKGFLKEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H8U7</accession>
    <name>A0A1A0H8U7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LrgB-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_43101</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711066.1">
        <property type="nucleotide sequence ID" value="XM_018857616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H8U7"/>
    <dbReference type="GeneID" id="30030592"/>
    <dbReference type="OrthoDB" id="2502820at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007300">
        <property type="entry name" value="CidB/LrgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249:SF0">
        <property type="entry name" value="PLASTIDAL GLYCOLATE_GLYCERATE TRANSLOCATOR 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30249">
        <property type="entry name" value="PUTATIVE SEROTONIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04172">
        <property type="entry name" value="LrgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="586"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA20544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="620" mass="69459" checksum="CC71438B791CB36D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H915</accession>
    <name>A0A1A0H915_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cenp-O kinetochore centromere component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_71196</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CENP-O/MCM21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018710894.1">
        <property type="nucleotide sequence ID" value="XM_018858395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H915"/>
    <dbReference type="STRING" id="869754.A0A1A0H915"/>
    <dbReference type="GeneID" id="30031371"/>
    <dbReference type="OrthoDB" id="10050372at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018464">
        <property type="entry name" value="CENP-O"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09496">
        <property type="entry name" value="CENP-O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="328" mass="36225" checksum="49009E02197241A0" modified="2016-10-05" version="1">MSLVDDLRDLETSIAALHEQIAPLQEKHDRMFREARDFEAAFKTKQLVRPVPGGRGPVPATADDAPPLIVHSHFDASIEKYFRTPALEPGPSTPAVLLRRQLQTAAGRVALAESIFRFGGITAFAINDRLYDQEDDALLGLRFDVLCHATLRFMAPHYIILRRRQPAAKTLESAPPWLVFRYTTPPYVPLDQLQDLLLQGDEGLQSFAEHVRALLVGVQYKHDKLDQLAGLSYAAVLGHGAADTIVSKLEKDLACTRAVLTMRRPAAAGGVLEIELLCGRLDVDAVHCTFGDARSELVVQTMLHGRSFQALAAAFSDVVRYLRETALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H996</accession>
    <name>A0A1A0H996_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DUF866-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_43948</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0587 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711092.1">
        <property type="nucleotide sequence ID" value="XM_018857721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H996"/>
    <dbReference type="STRING" id="869754.A0A1A0H996"/>
    <dbReference type="GeneID" id="30030697"/>
    <dbReference type="OrthoDB" id="10248838at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12857:SF0">
        <property type="entry name" value="CXXC MOTIF CONTAINING ZINC BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA20570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18513" checksum="E7BB6D51174B085F" modified="2016-10-05" version="1">MVKFYLKAQAELSNVTNLKPVDTPDSPFEYTFRIECTKCREIHGKPVTINRFEQHDISGSKGEASFVFRCKSCRNEHSAQISRTVESLSPENAGKWVNLLEIDARGADFIDFIPEGRWVCEALNSTSIFDEVDLEDGEWYDYDDNKGQEVSVTDFKWEISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9M9</accession>
    <name>A0A1A0H9M9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_32569</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711114.1">
        <property type="nucleotide sequence ID" value="XM_018856087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9M9"/>
    <dbReference type="STRING" id="869754.A0A1A0H9M9"/>
    <dbReference type="GeneID" id="30029063"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045254">
        <property type="term" value="C:pyruvate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007124">
        <property type="term" value="P:pseudohyphal growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000013">
        <property type="entry name" value="Pyruvate dehydrogenase E1 component subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017597">
        <property type="entry name" value="Pyrv_DH_E1_asu_subgrp-y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03182">
        <property type="entry name" value="PDH_E1_alph_y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="6">
        <location>
            <begin position="65"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA20592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="399" mass="44019" checksum="A4AC3D13B5C08C21" modified="2016-10-05" version="1">MLRHGAIRPLPRVLATSAQRGLASAASDLVNIQLPASSFEGYNLEQLPDLSFDTEKDSLLKMYKDMIIVRRMEMAADALYKAKKIRGFCHLSVGQEAIAVGIEYAITPKDTVITSYRCHGFAFMRGASVKSVLGELMGKRSGVSYGKGGSMHMFAPGFYGGNGIVGAQVPLGAGLAFAHKYKGEKNATFTLYGDGASNQGQVFEAYNMAKLWDLPCVFACENNRYGMGTSASRSSAMTEYYKRGQYIPGLKVNGMDILACYQASKFAKDWCASGNGPLVMEYETYRYGGHSMSDPGTTYRTREEVQQMRSRNDPIAGLKATLLELGIATEAEIKAYDKEARKYVDEQVAEAEKDSPPEAKMEILFEDVYVKGSEIPELRGRISDDTWNFEKNAFSNHVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0H9Q7</accession>
    <name>A0A1A0H9Q7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA topoisomerase</fullName>
            <ecNumber evidence="6">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_43242</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11 12">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711139.1">
        <property type="nucleotide sequence ID" value="XM_018857630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0H9Q7"/>
    <dbReference type="STRING" id="869754.A0A1A0H9Q7"/>
    <dbReference type="GeneID" id="30030606"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140226">
        <property type="term" value="F:RNA topoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006301">
        <property type="term" value="P:DNA damage tolerance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000018">
        <property type="term" value="P:regulation of DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.140:FF:000003">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="7">
        <location>
            <begin position="2"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="8">
        <location>
            <begin position="171"/>
            <end position="600"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA20617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="624" mass="69996" checksum="18D148BDC84203DA" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HA41</accession>
    <name>A0A1A0HA41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">Mevalonate kinase</fullName>
            <shortName evidence="18">MK</shortName>
            <ecNumber evidence="3 18">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">METBIDRAFT_41461</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Mevalonate kinase; part of the second module of ergosterol biosynthesis pathway that includes the middle steps of the pathway. The second module is carried out in the vacuole and involves the formation of farnesyl diphosphate, which is also an important intermediate in the biosynthesis of ubiquinone, dolichol, heme and prenylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="17 18">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 18"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA20870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711380.1">
        <property type="nucleotide sequence ID" value="XM_018857403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HA41"/>
    <dbReference type="STRING" id="869754.A0A1A0HA41"/>
    <dbReference type="GeneID" id="30030379"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000003">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 18 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 18" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 18" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16 18" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 18" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15 18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="19">
        <location>
            <begin position="124"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="20">
        <location>
            <begin position="294"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="OBA20870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="425" mass="45159" checksum="713C7B765D623397" modified="2016-10-05" version="1">MPDFFVSAPGKVILFGEHSAVYGKPAIAAALSLRAYLLVQRSADPDEIVLEFPDIGLVHKWTTKDMPWDELAAAVPRKNGGPAATDELVPELLDLAADLLVDCKLELHYTACRCFLYLYFNLCGPNVRGHRFCIRLTLPIGAGLGSLASTAVCLAAALATLGGHVGRPVHGASDVVLRKELSDAELIDAWSFMGEKCFHGNPSGIDNAVATHGGAVLYQRMADPSTPLIRTSIRNFAALRLLLINTRVPRSTAHLVGNVASLVAKHAKSTAAILDAMSHVATDAYQLMMSPSPDRASLRELVDINHGLLVALGVSHPSLEEVRVITERHQLGATKLTGAGGGGCAITVVDDAVSESDLDSVSKELAAKGFETFQAMLGGKGVGMLSADDCADTSIFTAESLAAYESREHIEQRIGASAVPGWKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HAB9</accession>
    <name>A0A1A0HAB9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_206354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000004">
        <property type="protein sequence ID" value="OBA20822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711344.1">
        <property type="nucleotide sequence ID" value="XM_018854991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HAB9"/>
    <dbReference type="GeneID" id="30027967"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA20822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="192" mass="20838" checksum="4909D385E1A0894F" modified="2016-10-05" version="1">MRSAACGMSSRPTCIQKQGRGQRPLGFISARHAGPAENCLRHDCGAGAPAHGQRPIKEMRRMEPGRKQTRGSHVRATFHPATAPRPGPGGARLRHAVVCVEKPQCLEFGAFIVRRAYSSACPYKAFFFSSLKREVDIFRAPRDKAHGPGSGLRAPADFTTPTRPETASPLGARMRQSPAPMDVSFCGGDRLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB00</accession>
    <name>A0A1A0HB00_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Subunit I/j of mitochondrial ATP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_69189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711573.1">
        <property type="nucleotide sequence ID" value="XM_018858375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB00"/>
    <dbReference type="STRING" id="869754.A0A1A0HB00"/>
    <dbReference type="GeneID" id="30031351"/>
    <dbReference type="OrthoDB" id="5520611at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033615">
        <property type="term" value="P:mitochondrial proton-transporting ATP synthase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006995">
        <property type="entry name" value="ATP_synth_F0_jsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28060:SF1">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT J, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04911">
        <property type="entry name" value="ATP-synt_J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="61" mass="6884" checksum="8AAE38B34D8AEB94" modified="2016-10-05" version="1">MRSYPTPILKNYWPFAVGAAISFGLIVKAAEALMNSDEFINDPRHPRFAKGGKFIDLEKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB27</accession>
    <name>A0A1A0HB27_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prefoldin alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_41381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711603.1">
        <property type="nucleotide sequence ID" value="XM_018857392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB27"/>
    <dbReference type="STRING" id="869754.A0A1A0HB27"/>
    <dbReference type="GeneID" id="30030368"/>
    <dbReference type="OrthoDB" id="10267474at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990113">
        <property type="term" value="P:RNA polymerase I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990114">
        <property type="term" value="P:RNA polymerase II core complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990115">
        <property type="term" value="P:RNA polymerase III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23157">
        <property type="entry name" value="Prefoldin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674:SF2">
        <property type="entry name" value="PREFOLDIN SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="149" mass="17050" checksum="DB7905BD793BCC09" modified="2016-10-05" version="1">MSQRRIDLSQLEPQQIVEIRKSTEEEIQHFTQSLKALQTAQLKLQDCISTIDNMDSTDNSALLVPMTSSLYLPGKIKDKGTYLVDIGTGYFAEKSSSEAKLVYQKKHTKLEGDSRKLKEILVQKNEIMNQINMVLRKIMMEQDQAAEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB34</accession>
    <name>A0A1A0HB34_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">p-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">METBIDRAFT_42535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711710.1">
        <property type="nucleotide sequence ID" value="XM_018857553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB34"/>
    <dbReference type="STRING" id="869754.A0A1A0HB34"/>
    <dbReference type="GeneID" id="30030529"/>
    <dbReference type="OrthoDB" id="10255969at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050334">
        <property type="entry name" value="Molybdenum_import_ModC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43514:SF4">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="10"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="290"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="OBA21200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="526" mass="59416" checksum="580211D9DB23AE3B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB40</accession>
    <name>A0A1A0HB40_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Amino acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_41312</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711607.1">
        <property type="nucleotide sequence ID" value="XM_018857382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB40"/>
    <dbReference type="STRING" id="869754.A0A1A0HB40"/>
    <dbReference type="GeneID" id="30030358"/>
    <dbReference type="OrthoDB" id="4476201at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649">
        <property type="entry name" value="AMINO-ACID PERMEASE BAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45649:SF6">
        <property type="entry name" value="GABA-SPECIFIC PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="354"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="434"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="504"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="567" mass="62078" checksum="2E55990A81553564" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB83</accession>
    <name>A0A1A0HB83_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_78291</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711781.1">
        <property type="nucleotide sequence ID" value="XM_018858856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HB83"/>
    <dbReference type="STRING" id="869754.A0A1A0HB83"/>
    <dbReference type="GeneID" id="30031832"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002889">
        <property type="entry name" value="WSC_carb-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16861">
        <property type="entry name" value="GLYCOPROTEIN 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16861:SF7">
        <property type="entry name" value="MEMBRANE ANCHOR OPY2 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01822">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00321">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51212">
        <property type="entry name" value="WSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291897" description="WSC domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="WSC" evidence="4">
        <location>
            <begin position="15"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA21271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="327" mass="33608" checksum="9AFCE8B8CF62C58F" modified="2016-10-05" version="1">MLYLAAWIWLAASLVSADEGCYSLVDTSVFEGTQQYGTQITCSTACGESYPYIAMKDGGECYCLSALPSSLDETSSSLCSVPCNGYGSETCGGTDAYTVFTNLEYLGTTLTSTTSLESLKSSTSPSSGSRPSAASSTTDNGSLSTSTTTINSSSFRTSSASGITSSGVHATTADSSTLSSSSSSASSAPSSASSSSSTSIGPIVGGVVGGLAALAFVTAIVFFFIRRKSKNHDEEEDYFKRGAAGGGGGSMSRAKSSKIDSVFDMPMVNPFEHPADELSEKRVSSAGVGGLTDPRLNPVMMGRRRISEGSLADEADYSRKILGVSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HB91</accession>
    <name>A0A1A0HB91_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_78280</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711765.1">
        <property type="nucleotide sequence ID" value="XM_018858854.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HB91"/>
    <dbReference type="GeneID" id="30031830"/>
    <dbReference type="OrthoDB" id="242910at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071561">
        <property type="term" value="C:nucleus-vacuole junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034271">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034272">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex, class III, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045324">
        <property type="term" value="P:late endosome to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055231">
        <property type="entry name" value="2AA_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045162">
        <property type="entry name" value="Vps15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17583:SF0">
        <property type="entry name" value="PHOSPHOINOSITIDE 3-KINASE REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22956">
        <property type="entry name" value="VPS15-like_hel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="27"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="625"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="1081"/>
            <end position="1122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="977"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="979"/>
            <end position="993"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1486" mass="166833" checksum="00AEEF3D6CFD7216" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBA6</accession>
    <name>A0A1A0HBA6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein RIX1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">METBIDRAFT_19087</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA and the nucleoplasmic transit of the pre-60S ribosomal subunits. Regulates pre-60S association of the critical remodeling factor MDN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RIX1/PELP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711924.1">
        <property type="nucleotide sequence ID" value="XM_018854843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBA6"/>
    <dbReference type="STRING" id="869754.A0A1A0HBA6"/>
    <dbReference type="GeneID" id="30027819"/>
    <dbReference type="OrthoDB" id="20900at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012583">
        <property type="entry name" value="RIX1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34105:SF1">
        <property type="entry name" value="PROLINE-, GLUTAMIC ACID- AND LEUCINE-RICH PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08167">
        <property type="entry name" value="RIX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Pre-rRNA-processing protein RIX1 N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="437"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="OBA21414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="483" mass="53010" checksum="F3CFDF3BAF0ADA77" modified="2016-10-05" version="1" fragment="single">MSLPLAVVIDQLKSTPASVIPILSALDKAKSTFASVSKVDLKHLTSRTLNLCRSNDPYSVWCGINIIYVLADSSEIVSAEGSAFFGQLLKVLGSQHAQNKAVLESAIECLARLCDSIRGKPTLTREILTPNLSSVFTVLLDKLALSPYLVLGSLKKLIQEHPTTSRPFANKLKTHILTFVMEAQFLSYPQKLRATACQTLACLPVIEKDAPEQHWLRDVHALISELTSTVEIYASFLNVQDDQEASTLLKSLGSTDHESLFPLLSIDINEPSSIFAISQRVGVLFELLQGYLLSGTKFPVVVPIGRVLSVVDLVCSLNTRFVSFKREIRDADVKQYVDISLTQCHFSALGLLQQLPATFCALLVPHMLAVLATLELLVFLQNKRLDHARILANEPMICEVIQCTSSFLGLTSFFADHSLISRILEAAVFLVQPRAGGQQLAGDGGKVKLSNQSKSARKKTKRNAAVPLSDLISHEHLFRQTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HBT1</accession>
    <name>A0A1A0HBT1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_188469</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712104.1">
        <property type="nucleotide sequence ID" value="XM_018854819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HBT1"/>
    <dbReference type="GeneID" id="30027795"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="131" mass="14451" checksum="C7A9ADD27CD5CAAE" modified="2016-10-05" version="1">MYEYYLMVLALMPKRFGVAKWCVGLVRASGHILVFYVPGATCCCALIEGPWLDIVVLISILVLVLTGMFNRLLTNRYFEFAANKDAVLNLLLTIMRVRNLILLALGEVSLVSTGAIIFNLLGLLARLASVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC02</accession>
    <name>A0A1A0HC02_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Vesicle tethering protein Uso1/P115-like head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_31942</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018711928.1">
        <property type="nucleotide sequence ID" value="XM_018855949.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HC02"/>
    <dbReference type="GeneID" id="30028925"/>
    <dbReference type="OrthoDB" id="4094778at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048211">
        <property type="term" value="P:Golgi vesicle docking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024095">
        <property type="entry name" value="Vesicle_P115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006953">
        <property type="entry name" value="Vesicle_Uso1_P115_head"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10013:SF0">
        <property type="entry name" value="GENERAL VESICULAR TRANSPORT FACTOR P115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04869">
        <property type="entry name" value="Uso1_p115_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vesicle tethering protein Uso1/P115-like head" evidence="6">
        <location>
            <begin position="383"/>
            <end position="737"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="476"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1089"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1897"/>
            <end position="1985"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="754"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1208"/>
            <end position="1359"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1426"/>
            <end position="1460"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1485"/>
            <end position="1512"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1543"/>
            <end position="1697"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="1750"/>
            <end position="1804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="484"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1089"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1107"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1117"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1897"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1945"/>
            <end position="1973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1974"/>
            <end position="1985"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA21418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="2022" mass="230007" checksum="4EDED83AEFC6DF18" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC03</accession>
    <name>A0A1A0HC03_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_187463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712051.1">
        <property type="nucleotide sequence ID" value="XM_018854808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC03"/>
    <dbReference type="GeneID" id="30027784"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="188" mass="23218" checksum="CF38548423B0D521" modified="2016-10-05" version="1">MGRVLGEWIYFCAWVCVGLYFWWFIFAWFCSLTWLCSFAWLYFLRGFIFLRGFIFCVALFFCVALFFAWLYFFAWLYFFAWLYFFAWLYFFAWLYFFAWFCSSGGFVLLRGFVLLCDFILCVALFFYATLFSTWLRSFMWLHFLASLLAWLIWICRCVFMAAFMFFCCVYSQAWWWLVLTAVRYYLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC41</accession>
    <name>A0A1A0HC41_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Trafficking protein particle complex subunit 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_42364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712214.1">
        <property type="nucleotide sequence ID" value="XM_018857536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC41"/>
    <dbReference type="STRING" id="869754.A0A1A0HC41"/>
    <dbReference type="GeneID" id="30030512"/>
    <dbReference type="OrthoDB" id="6278596at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021773">
        <property type="entry name" value="TPC11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374">
        <property type="entry name" value="FOIE GRAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14374:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11817">
        <property type="entry name" value="Foie-gras_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 11" evidence="1">
        <location>
            <begin position="309"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1156" mass="129767" checksum="8B73C146DF70417C" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC49</accession>
    <name>A0A1A0HC49_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_41078</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712096.1">
        <property type="nucleotide sequence ID" value="XM_018857350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC49"/>
    <dbReference type="GeneID" id="30030326"/>
    <dbReference type="OrthoDB" id="4003242at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA21586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="150" mass="17062" checksum="3957C365EC5204EF" modified="2016-10-05" version="1">MFRATSRTCRASFKRFFAAGAQHDAPAATQTEIDVTKVFSMAVLAGVSLYFYRSSDSPVVETAYYKQMENRDQMRSDAYLKRYKTSFIKTFIRDKGGLGQNHLRAAAHTPIPQTFIHLHSPYGQQFGAGIKTDKLGPRRERIKYFAPLQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HC95</accession>
    <name>A0A1A0HC95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C-1-tetrahydrofolate synthase, cytoplasmic</fullName>
            <ecNumber evidence="8">1.5.1.5</ecNumber>
            <ecNumber evidence="7">3.5.4.9</ecNumber>
            <ecNumber evidence="6">6.3.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">METBIDRAFT_12022</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(6R)-5,10-methenyltetrahydrofolate + H2O = (6R)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:23701"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:23702"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:22813"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:22814"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(6S)-5,6,7,8-tetrahydrofolate + formate + ATP = (6R)-10-formyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20221"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:20222"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:20223"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="25">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="8"/>
    <dbReference type="EC" id="3.5.4.9" evidence="7"/>
    <dbReference type="EC" id="6.3.4.3" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712033.1">
        <property type="nucleotide sequence ID" value="XM_018854149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HC95"/>
    <dbReference type="STRING" id="869754.A0A1A0HC95"/>
    <dbReference type="GeneID" id="30027125"/>
    <dbReference type="OrthoDB" id="5126881at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004329">
        <property type="term" value="F:formate-tetrahydrofolate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00477">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000006">
        <property type="entry name" value="Bifunctional protein FolD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000245">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001123">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10860:FF:000005">
        <property type="entry name" value="C-1-tetrahydrofolate synthase, cytoplasmic, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.410.10:FF:000001">
        <property type="entry name" value="Putative formate--tetrahydrofolate ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.410.10">
        <property type="entry name" value="Formyltetrahydrofolate synthetase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01543">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000559">
        <property type="entry name" value="Formate_THF_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020628">
        <property type="entry name" value="Formate_THF_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099:SF5">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48099">
        <property type="entry name" value="C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01268">
        <property type="entry name" value="FTHFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00721">
        <property type="entry name" value="FTHFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00722">
        <property type="entry name" value="FTHFS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="17" id="KW-0521">NADP</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase catalytic" evidence="23">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydrofolate dehydrogenase/cyclohydrolase NAD(P)-binding" evidence="24">
        <location>
            <begin position="143"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00017592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF02882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="OBA21523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="946" mass="100847" checksum="80CC97175F829C6B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCC6</accession>
    <name>A0A1A0HCC6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Small ribosomal subunit protein mS35 mitochondrial conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_40809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712155.1">
        <property type="nucleotide sequence ID" value="XM_018857317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCC6"/>
    <dbReference type="STRING" id="869754.A0A1A0HCC6"/>
    <dbReference type="GeneID" id="30030293"/>
    <dbReference type="OrthoDB" id="283424at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019349">
        <property type="entry name" value="Ribosomal_mS35_mit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039848">
        <property type="entry name" value="Ribosomal_mS35_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13490:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10213">
        <property type="entry name" value="MRP-S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small ribosomal subunit protein mS35 mitochondrial conserved" evidence="1">
        <location>
            <begin position="142"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="300" mass="34336" checksum="61AC315DBE3DE1ED" modified="2016-10-05" version="1">MKNSGLRHVAALRHQSQAGSSLYLNPHKWQGLPADRVFELHEMRKTALGEKYAPNDAERQAILATYAELKVASPALAYSYEIDNFAERVMNNTPVALRGLPKKYSNVPVLDKGATPHEKRRLEQLHRISAYEMPLLAKYRQPYTPQPAAQTPLKLKFTTDFSDESTAVNRSVALSVKLQHLGLTDRQATKFKLLAGDKFDHRSNTFRLSTDRYPESTQNARWLVETFNRLLRESKDLTKNTFDEVPLNNVRQSARRKAAVFPELWKRPQDAPVQKHRIVKECVDAVKQRMDAEYVKSLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCM9</accession>
    <name>A0A1A0HCM9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydropantolactone reductase</fullName>
            <ecNumber evidence="6">1.1.1.358</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-dehydropantolactone reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ketopantoyl-lactone reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_78551</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-pantolactone + NADP(+) = 2-dehydropantolactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16719"/>
            <dbReference type="ChEBI" id="CHEBI:18395"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.358"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>isatin + NADPH + H(+) = 3-hydroxyindolin-2-one + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:68608"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27539"/>
            <dbReference type="ChEBI" id="CHEBI:28536"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.358" evidence="6"/>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712157.1">
        <property type="nucleotide sequence ID" value="XM_018858898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCM9"/>
    <dbReference type="STRING" id="869754.A0A1A0HCM9"/>
    <dbReference type="GeneID" id="30031874"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047011">
        <property type="term" value="F:2-dehydropantolactone reductase (A-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042180">
        <property type="term" value="P:ketone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF3">
        <property type="entry name" value="NADP-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="12">
        <location>
            <begin position="50"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="11">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00051098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00066965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA21647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="326" mass="36809" checksum="4CEDF7491C90EA66" modified="2016-10-05" version="1">MTRTQPVEPFGTKLTTKSGNAVRLGTGSGTKWQIIKKARDASLQNTLVDELVDNLVTSVQNGFRHLDTAEIYTIHPEIAEAIKKSGVAREDLFVTTKYNPGIEQRHAFYKSGAESVDAALKEPDVEYIDLFLIHYPFFQKEYSHGQSLEDVWADLIQAKKDGKVRFIGGSNFTVDHLKLLSLVAGGDPEYSPQVNQIEFHPYLQNQSEGIREFAKENEILLEAYGPLSPLFRIVDSDGKEVVDHPLKPVMPRLAEKYGKTDAQILLRYTLQKGLLPITTSLQSDRQKEALAVYDFALDDEDVQLIDDTGSQFHFRGFFKNLYHTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCR7</accession>
    <name>A0A1A0HCR7_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LSM complex subunit LSM4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LSM4</name>
        <name evidence="12" type="ORF">METBIDRAFT_12184</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000003">
        <property type="protein sequence ID" value="OBA21708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712218.1">
        <property type="nucleotide sequence ID" value="XM_018854187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HCR7"/>
    <dbReference type="STRING" id="869754.A0A1A0HCR7"/>
    <dbReference type="GeneID" id="30027163"/>
    <dbReference type="OrthoDB" id="747253at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042149">
        <property type="term" value="P:cellular response to glucose starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="2"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="86"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA21708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="146" mass="16901" checksum="2306E963259538B8" modified="2016-10-05" version="1">MLPLYMLTSIKKQEILVELKNGETVYGKLNNCDSWMNLTLTDVVYNFNNGEKFQKMNEIYVRGAHIKFLRLPDLVMDQAKEQNMMNMEQRNRNQKRRGTGGFQRRNYNNDYSRDNRDNRNFNGSLRAGGNGGNGGNGGYGRRNYAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HCS1</accession>
    <name>A0A1A0HCS1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BAH-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_16615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712281.1">
        <property type="nucleotide sequence ID" value="XM_018854660.1"/>
    </dbReference>
    <dbReference type="STRING" id="869754.A0A1A0HCS1"/>
    <dbReference type="GeneID" id="30027636"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048189">
        <property type="term" value="C:Lid2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036205">
        <property type="term" value="P:histone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15497">
        <property type="entry name" value="PHD1_Snt2p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029617">
        <property type="entry name" value="Snt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00439">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51038">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BAH" evidence="7">
        <location>
            <begin position="137"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="323"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="1107"/>
            <end position="1163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="395"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="503"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="890"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="900"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="952"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="995"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA21785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1393" mass="155137" checksum="04FB605570D3F4D1" modified="2016-10-05" version="1" fragment="single"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HD95</accession>
    <name>A0A1A0HD95_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSN8/PSMD8/EIF3K domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_38975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA21903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712399.1">
        <property type="nucleotide sequence ID" value="XM_018857075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HD95"/>
    <dbReference type="STRING" id="869754.A0A1A0HD95"/>
    <dbReference type="GeneID" id="30030051"/>
    <dbReference type="OrthoDB" id="2100128at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39398:SF1">
        <property type="entry name" value="CSN8_PSMD8_EIF3K DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39398">
        <property type="entry name" value="YALI0F14311P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA21903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="35629" checksum="A7313B7623D20D96" modified="2016-10-05" version="1">MRFYAGNPGAGRSNSDGKLVDPALYGYISRGEDSRLQHSETAQREYFDWILETFRQVENSGLAAVAECAEKMSADLGIKGSSASSEQVLPSLRKLREALLHSPPSEFSKKVHLFSVRVSSAVGHYQTYVPSIQYLLADGRSLLTRTERKEMASILVLHVTHRTGDTSEGFRLFFQYLNPHKECRILQAIVAWAQGDYHAWMELYNSESDHSVFAIMTMGLATMMRHMIECFNGAYYTYSLEDLERCLPKGVTWAEFRERYNVPWELEDNTVHIRRRQVRGKRVDNAEKGGGGLRGDARPLREAGPKGGPGAVSGADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDW6</accession>
    <name>A0A1A0HDW6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_77736</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for protein translocation in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712785.1">
        <property type="nucleotide sequence ID" value="XM_018858752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDW6"/>
    <dbReference type="STRING" id="869754.A0A1A0HDW6"/>
    <dbReference type="GeneID" id="30031728"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006398">
        <property type="entry name" value="Sec61_beta_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="90" mass="9371" checksum="BC494350F96517A1" modified="2016-10-05" version="1">MSSSAAPGGLRNAVKRRNVADKKAKNAELMPLSTRAAGAGGSSATMLKLFTDEAQGLRVDPLVVLILAVGFIFSVIVMHVLAKITGKFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDX2</accession>
    <name>A0A1A0HDX2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome b-c1 complex subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_148564</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the UQCRB/QCR7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712707.1">
        <property type="nucleotide sequence ID" value="XM_018854549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDX2"/>
    <dbReference type="STRING" id="869754.A0A1A0HDX2"/>
    <dbReference type="GeneID" id="30027525"/>
    <dbReference type="OrthoDB" id="425749at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099617">
        <property type="term" value="C:matrix side of mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1090.10:FF:000001">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1090.10">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003197">
        <property type="entry name" value="QCR7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036544">
        <property type="entry name" value="QCR7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022:SF0">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12022">
        <property type="entry name" value="UBIQUINOL-CYTOCHROME C REDUCTASE COMPLEX 14 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02271">
        <property type="entry name" value="UCR_14kD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000022">
        <property type="entry name" value="Bc1_14K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81524">
        <property type="entry name" value="14 kDa protein of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="OBA22211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="124" mass="14043" checksum="582D1AA7003B7CA4" modified="2016-10-05" version="1">MASITSIVKASDWILSRPTLSKVVLPVAKTFCAYAGYREMGLKFNDLIAEENPIAQKAISRLPEDQLYARNFRTLTAHQLALSHQILPPNKAVQPEEDTHYLIPYLLEAEKEAFEKAELDQMKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HDX9</accession>
    <name>A0A1A0HDX9_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_39711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712800.1">
        <property type="nucleotide sequence ID" value="XM_018857169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HDX9"/>
    <dbReference type="STRING" id="869754.A0A1A0HDX9"/>
    <dbReference type="GeneID" id="30030145"/>
    <dbReference type="OrthoDB" id="4062523at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF6">
        <property type="entry name" value="PROTEIN ECM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="319" mass="34285" checksum="2DDF5626C2226F4B" modified="2016-10-05" version="1" fragment="single">MVSVTKLLGTAARPFRGLTPAQKRLLLLRIGSSLVAVCIAVVLYALRSYPSSLYVARINCSQLDVAAGLYQSLRSLMTETIISKGSGSNVLPIDLALTDSEVSILTQYAQTQVLNAPQAIVLGAQDWCTVNYATDFAGRLRDYSNMSTTCYEYGSLNFYDYRTLLLQNQLEIILAYAYESEDVQSAAYKERIQERSARYDVMRIMYAVQLALQVVLLAAGVFIYGTRGGAPDLAHTSAVTLNAVALVALTAGSTMIVSTAIVLADLAAMRAEIRRGLGDFGILMSVGPVFLALAAAALAVASLGMLAWIVPLWCANPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HE06</accession>
    <name>A0A1A0HE06_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein HGH1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_77698</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HGH1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712742.1">
        <property type="nucleotide sequence ID" value="XM_018858746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HE06"/>
    <dbReference type="STRING" id="869754.A0A1A0HE06"/>
    <dbReference type="GeneID" id="30031722"/>
    <dbReference type="OrthoDB" id="338814at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061770">
        <property type="term" value="F:translation elongation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039717">
        <property type="entry name" value="Hgh1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007206">
        <property type="entry name" value="Protein_HGH1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007205">
        <property type="entry name" value="Protein_HGH1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13387:SF9">
        <property type="entry name" value="PROTEIN HGH1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04063">
        <property type="entry name" value="DUF383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04064">
        <property type="entry name" value="DUF384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein HGH1 N-terminal" evidence="3">
        <location>
            <begin position="96"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Protein HGH1 C-terminal" evidence="4">
        <location>
            <begin position="289"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="360" mass="41135" checksum="47ABDA26475EC998" modified="2016-10-05" version="1">MPTELEELVEFLHSPQPAVVQIALDNLVGYSQGPQQKVFAYNNYEAIKDLKKLSRDTGKTSVSQSVTILANLCDDSTVRELIIKDKDYLSFLVSAIIRPTNINADLMSILLANLAKNDLITSIFEIEVKHTDEENKFFKLTKAMDCLMDCFVKGFDRSLNKYANYDYLAYFFSDISRLKMGRAYFITEQSYDEVVPISKLLVFTEKYDSKIRREGVASTIKNSLFDIDSHGTLITDEGINLLPYILLPLAGPEELDEDDMFNLPDELQLLPSDKTREPVETILNTHLESLLLLSTSKEIREYLRAKSVYALIRELHKVSDSEDVTALCDRLVQLLMRDEEVTRENVDESDEDEDQIVEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEB4</accession>
    <name>A0A1A0HEB4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DUF676-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">METBIDRAFT_77694</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712738.1">
        <property type="nucleotide sequence ID" value="XM_018858745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEB4"/>
    <dbReference type="GeneID" id="30031721"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF24">
        <property type="entry name" value="LIPID DROPLET PHOSPHOLIPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="5">
        <location>
            <begin position="14"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="351"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="OBA22242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="567" mass="65022" checksum="5FC6E8119036A5B4" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEE5</accession>
    <name>A0A1A0HEE5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_153984</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712980.1">
        <property type="nucleotide sequence ID" value="XM_018854595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEE5"/>
    <dbReference type="STRING" id="869754.A0A1A0HEE5"/>
    <dbReference type="GeneID" id="30027571"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013586">
        <property type="entry name" value="PSMD3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057985">
        <property type="entry name" value="TPR_PSMD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08375">
        <property type="entry name" value="Rpn3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25573">
        <property type="entry name" value="TPR_PSMD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="4">
        <location>
            <begin position="240"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="486" mass="53404" checksum="43F0CF3490D05FB7" modified="2016-10-05" version="1">MSAPTDVDMKDASLEAPKDAPGAPETPSLAQEIKLAFGSLHKAADNFDNRYVSKVFRDLGALRRKIAQDPQALAAVVEQTYPGTHKSKAVLQAPLPAPAGGTVAVDDPLKAPVVPEVDAYLHLLVQIYLLDTAQMAALAAFNGHVAAWLAAHDRRTLDFLQAKMWFYVARAAEVTGRLLDVRPALTGALRSATLRRDDETTAAVITLLLRNYLLSHDVSQAANLCEKSVFPAAAGNALAARYYYYVARIHAVQLDYSAAHECVTAAMRKAPQTALARGFVQQATKLNVLVELLMGDVPALSVFKAAPGRLEPYFLVTKAVRRGDLAGFAEVLRAHEPVFVADNNLTLVARLRQNVIKTGIRMLSLSYSRISLRDVCIRLRLDSEEATEYIVSKAVRDGVIEASVDHLLGHMKLRELLDVYSTRQPQEDFDQRIRFCLSLHTDSVKAMRYPADDSKNEMNRGPLEGLEDEMGLLQAIEDGDLDDFMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HEE6</accession>
    <name>A0A1A0HEE6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein EFR3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">METBIDRAFT_40283</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EFR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018712862.1">
        <property type="nucleotide sequence ID" value="XM_018857252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HEE6"/>
    <dbReference type="STRING" id="869754.A0A1A0HEE6"/>
    <dbReference type="GeneID" id="30030228"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039786">
        <property type="entry name" value="EFR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049150">
        <property type="entry name" value="EFR3_HEAT-like_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47766:SF1">
        <property type="entry name" value="PROTEIN EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21072">
        <property type="entry name" value="EFR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="780"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="OBA22366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="878" mass="94087" checksum="827C1B7523B20946" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF54</accession>
    <name>A0A1A0HF54_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_228140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713245.1">
        <property type="nucleotide sequence ID" value="XM_018855196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF54"/>
    <dbReference type="STRING" id="869754.A0A1A0HF54"/>
    <dbReference type="GeneID" id="30028172"/>
    <dbReference type="OrthoDB" id="6287725at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030427">
        <property type="term" value="C:site of polarized growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039867">
        <property type="entry name" value="Furry/Tao3/Mor2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295">
        <property type="entry name" value="FURRY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12295:SF30">
        <property type="entry name" value="PROTEIN FURRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="134" mass="15691" checksum="EA8B776B4E2424C1" modified="2016-10-05" version="1">MVPLQVNFTGREEESDFPYSGNLTHTLGLNFKELAFSLGQLSMVFLVDLFRFKNHKTLDHLPLLLRISFSLMDQYFPLVQEQTVSLLMHTMHSIAPDHPKAKRFVETLRKRDDLTRLWTYDLNNDKKGALLQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HF94</accession>
    <name>A0A1A0HF94_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_28339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713290.1">
        <property type="nucleotide sequence ID" value="XM_018855352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HF94"/>
    <dbReference type="STRING" id="869754.A0A1A0HF94"/>
    <dbReference type="GeneID" id="30028328"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000131">
        <property type="term" value="C:incipient cellular bud site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032878">
        <property type="term" value="P:regulation of establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031106">
        <property type="term" value="P:septin ring organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012104">
        <property type="entry name" value="PHO85_cyclin_1/2/9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF114">
        <property type="entry name" value="PHO85 CYCLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016511">
        <property type="entry name" value="Cyclin_Pcl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="1">
        <location>
            <begin position="49"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA22809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="182" mass="20636" checksum="32AADFDF876411D8" modified="2016-10-05" version="1" fragment="single">MTSSLDLKALNVFIRTPVSQDMVHKLVVATLQVIQCQEDKNSKSTNGGKPLPSLMSFINKLVKYTNVYTGTLMATLVLLGRLKLKLPHNAQGLACTRHRVFLSCLILALKFHNDSSPKNVHWAKYTDGLFSLKDVNLMEVQLLFLLNWNVKVSNEDMVLHLRKFLAPIKDDLVKTARMKQFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFB0</accession>
    <name>A0A1A0HFB0_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">COP9 signalosome complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">METBIDRAFT_39143</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the COP9 signalosome (CSN) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M67A family. CSN5 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713069.1">
        <property type="nucleotide sequence ID" value="XM_018857100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFB0"/>
    <dbReference type="STRING" id="869754.A0A1A0HFB0"/>
    <dbReference type="GeneID" id="30030076"/>
    <dbReference type="OrthoDB" id="605656at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019784">
        <property type="term" value="F:deNEDDylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000338">
        <property type="term" value="P:protein deneddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000203">
        <property type="entry name" value="COP9 signalosome complex subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0736">Signalosome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="15">
        <location>
            <begin position="55"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="194"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="16">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00014880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="OBA22573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="260" mass="28727" checksum="EEA5D373E4168486" modified="2016-10-05" version="1" fragment="single">MGAIDNPKALRVSLTKKKLSELSVAKSHSLYELPTDDNALDTRPWRNLPKYFTQVLISTVALVKMCAHAKLGGSIEIMGMLTGKIVGDLFIVMDVYSLPVEGTETRVNAQSEAYEFMVQYLDNLKEVKPKDSIVGWYHSHPGYGCWLSGIDVATQSLNQNFQDPYLAIVVDPSRTLNQGKVEIGAFRTFPVGYTDGKSSQGHEATSRRPAGAKKSPENMGAHSDQYYSLDIKLFKGPHDDEIVNSILDKSWISGIVQTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFH8</accession>
    <name>A0A1A0HFH8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Nascent polypeptide-associated complex subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">METBIDRAFT_39646</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the nascent polypeptide-associated complex (NAC), a dynamic component of the ribosomal exit tunnel, protecting the emerging polypeptides from interaction with other cytoplasmic proteins to ensure appropriate nascent protein targeting. The NAC complex also promotes mitochondrial protein import by enhancing productive ribosome interactions with the outer mitochondrial membrane and blocks the inappropriate interaction of ribosomes translating non-secretory nascent polypeptides with translocation sites in the membrane of the endoplasmic reticulum. EGD1 may act as a transcription factor that exert a negative effect on the expression of several genes that are transcribed by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the nascent polypeptide-associated complex (NAC), consisting of EGD2 and EGD1. NAC associates with ribosomes via EGD1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the NAC-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000002">
        <property type="protein sequence ID" value="OBA22618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713114.1">
        <property type="nucleotide sequence ID" value="XM_018857163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFH8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFH8"/>
    <dbReference type="GeneID" id="30030139"/>
    <dbReference type="OrthoDB" id="8033832at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005854">
        <property type="term" value="C:nascent polypeptide-associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006613">
        <property type="term" value="P:cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22055">
        <property type="entry name" value="NAC_BTF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.70.30:FF:000001">
        <property type="entry name" value="Transcription factor BTF3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039370">
        <property type="entry name" value="BTF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10351">
        <property type="entry name" value="TRANSCRIPTION FACTOR BTF3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="12">
        <location>
            <begin position="32"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="123"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="OBA22618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="151" mass="16327" checksum="DFD5268886ABBC54" modified="2016-10-05" version="1">MPIDQEKLAKLQKAGPRKVGGARVKAKKVKSESDDVKLMETLGKFKAIKVENVEEANFFKDDGKVLHFPRVGVQAASASNTFAFSGYPQEKDITQLIPNILPHLGAENLDVLRNLAEQIQAGKAPTELNAGGAGEDDIPDLVAGEKFDDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFP8</accession>
    <name>A0A1A0HFP8_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-conjugating enzyme E2 H</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">(E3-independent) E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin carrier protein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-protein ligase H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">METBIDRAFT_36872</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with MAEA and WDR26, components of the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713307.1">
        <property type="nucleotide sequence ID" value="XM_018856791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFP8"/>
    <dbReference type="STRING" id="869754.A0A1A0HFP8"/>
    <dbReference type="GeneID" id="30029767"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070096">
        <property type="term" value="P:mitochondrial outer membrane translocase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000078">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="2"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="14">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="OBA22826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="181" mass="20532" checksum="A50B451001A8E30E" modified="2016-10-05" version="1">MSAKRRIEKDVMDLMMSDFDVSLIDDSMQQFHVIFHGPSDTPYAGGVWKLRVELPDQYPIKLPLIGFVNKIYHPNIDETSGSVCLDVINQTWSPMFGLLNIFENFLPHLLRYGNPSDPLNTEALGLMTQNEEKYIETVKKYVQQYALQNLVTDELIEAAVENDDGDLSDVGSLSSDEDEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFV5</accession>
    <name>A0A1A0HFV5_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29444</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713346.1">
        <property type="nucleotide sequence ID" value="XM_018855402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFV5"/>
    <dbReference type="STRING" id="869754.A0A1A0HFV5"/>
    <dbReference type="GeneID" id="30028378"/>
    <dbReference type="OrthoDB" id="5324744at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="722" mass="80680" checksum="A8D9ED50BEE0C037" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HFY9</accession>
    <name>A0A1A0HFY9_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TRP C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">METBIDRAFT_228187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713293.1">
        <property type="nucleotide sequence ID" value="XM_018855197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HFY9"/>
    <dbReference type="STRING" id="869754.A0A1A0HFY9"/>
    <dbReference type="GeneID" id="30028173"/>
    <dbReference type="OrthoDB" id="5312224at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010308">
        <property type="entry name" value="TRP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040241">
        <property type="entry name" value="TRP_Flc/Pkd2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31145:SF6">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_7G01610)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06011">
        <property type="entry name" value="TRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="447"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="504"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="580"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="662"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="724"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="750"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="820"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="TRP C-terminal" evidence="2">
        <location>
            <begin position="487"/>
            <end position="845"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="OBA22812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1044" mass="116591" checksum="48B6673E6A16D44B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG03</accession>
    <name>A0A1A0HG03_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Hyphally-regulated cell wall protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_9402</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Cell wall</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713570.1">
        <property type="nucleotide sequence ID" value="XM_018859381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG03"/>
    <dbReference type="STRING" id="869754.A0A1A0HG03"/>
    <dbReference type="GeneID" id="30032356"/>
    <dbReference type="OrthoDB" id="4022214at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021031">
        <property type="entry name" value="Hyphal-reg_cell_wall_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11765">
        <property type="entry name" value="Hyphal_reg_CWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5008291825" description="Hyphally-regulated cell wall protein N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Hyphally-regulated cell wall protein N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="338" mass="37498" checksum="9C9FFD49CBF7EC08" modified="2016-10-05" version="1">MKFGIYVVAISWLLSRVFAVNIQQSTVEIDQGVKAIENLTIHKIVYYSIINSPQVTVLHSFKNAGTFYVTSTNGFEASVVIKGDHFQNSGLVVFNSFLVNESIHGVDVENDFANTGAMLFGVSGFNPYSSVTFVLSDGSWVNTGMISFLKLSEYPTRLYIGRSQRLKGGLSITNEGTICFYSQIWASYTRIKGHGCITVGSKSKLEIYFPDYRISPTQTINLESSDSLLQVYGMSSSLDIAPTIKVSGLGEGNSIEVDIPYLTMNAKEYSTRRGELSVEIKSKPRVYFRVGRGYRLVDFQIRPSAFGTRITVHKPAPRKPPRICKCATKIPVVPTYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-02-05" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG27</accession>
    <name>A0A1A0HG27_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_36621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713595.1">
        <property type="nucleotide sequence ID" value="XM_018856764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG27"/>
    <dbReference type="GeneID" id="30029740"/>
    <dbReference type="OrthoDB" id="4004290at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA23114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="56" mass="6238" checksum="DD783A7BAC3EE549" modified="2016-10-05" version="1" fragment="single">QYSLMYAAVTSKTSVLTLGYLTVSTMVPFVLSYKETQGNSVYTKGYISSRSLFNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG87</accession>
    <name>A0A1A0HG87_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">METBIDRAFT_29451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713354.1">
        <property type="nucleotide sequence ID" value="XM_018855405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG87"/>
    <dbReference type="GeneID" id="30028381"/>
    <dbReference type="OrthoDB" id="10462389at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="OBA22873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="113" mass="14024" checksum="3F2B774D6D059F07" modified="2016-10-05" version="1">MKRWRKFWKDFYKQEWLVQNDLTAIHLFNHGSYVPLHDDEVWKSRFECRLCLKRCSSDQKLTHLYNECETVHFWWKKSSFPARLRSREMLAPLDTFFDCRRNPNWLVKVVRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-06-18" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HG93</accession>
    <name>A0A1A0HG93_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_29555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713491.1">
        <property type="nucleotide sequence ID" value="XM_018855436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HG93"/>
    <dbReference type="STRING" id="869754.A0A1A0HG93"/>
    <dbReference type="GeneID" id="30028412"/>
    <dbReference type="OrthoDB" id="270639at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016150">
        <property type="term" value="F:translation release factor activity, codon nonspecific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070126">
        <property type="term" value="P:mitochondrial translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052104">
        <property type="entry name" value="Mito_Release_Factor_mL62"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075:SF54">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11075">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110916">
        <property type="entry name" value="Peptidyl-tRNA hydrolase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="1">
        <location>
            <begin position="37"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="179" mass="21033" checksum="F8D071493BBF79FE" modified="2016-10-05" version="1">MLRYGFSQFRVYSTRPVHSNEEVAVAKKWLRDFQSEHIPRDPFSITYSRSSGPGGQKVNKTLSKATVSLEAFQWLNPRICSWIPVPIIRQIAATKFRYLTKMGGLHIQSDTSRSREVNTDECFRKLLKEIKLVVYFEEDASEEDKKKWQKLAALLKEYRLQDKKRKSDKKKARSKKFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGD2</accession>
    <name>A0A1A0HGD2_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19 20">CDP-diacylglycerol--inositol 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="5 20">2.7.8.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">METBIDRAFT_76062</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>a CDP-1,2-diacyl-sn-glycerol + myo-inositol = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11580"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20 21">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.11" evidence="5 20"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA22913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713394.1">
        <property type="nucleotide sequence ID" value="XM_018858471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGD2"/>
    <dbReference type="STRING" id="869754.A0A1A0HGD2"/>
    <dbReference type="GeneID" id="30031447"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000021">
        <property type="entry name" value="CDP-diacylglycerol--inositol 3-phosphatidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014387">
        <property type="entry name" value="CDP_diag_ino_3_P_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000848">
        <property type="entry name" value="CDP_diag_ino_3_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 20 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 20" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="17 20" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="OBA22913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="213" mass="24509" checksum="4BCEBC0B2DA96DC4" modified="2016-10-05" version="1">MADNQKITVQHVFRFIPNVIGYIRVLTAFLSFLTMKNHPVWTLVLYGISGFLDAFDGYAARKFDQGTRFGAVLDMVTDRCATSSLICYLCVIYPQYCVMWQLLVSLDLSSHYMHMYAMLSSGSTSHKNVGEKSKLLNLYYTNRKVLFFVCLVNELFYMATYLHYYNFFWMGTCLGWVSAPIWIFKQIANVVQLKNAALILAEKDALERSTKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGF4</accession>
    <name>A0A1A0HGF4_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS general substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_76181</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713567.1">
        <property type="nucleotide sequence ID" value="XM_018858486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGF4"/>
    <dbReference type="STRING" id="869754.A0A1A0HGF4"/>
    <dbReference type="GeneID" id="30031462"/>
    <dbReference type="OrthoDB" id="5376138at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000088">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="89"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="536"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="558" mass="62370" checksum="24A4B975D4683E44" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGI1</accession>
    <name>A0A1A0HGI1_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_99060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713592.1">
        <property type="nucleotide sequence ID" value="XM_018859484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGI1"/>
    <dbReference type="GeneID" id="30032459"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="119" mass="13518" checksum="8F03CEB5CD7E714C" modified="2016-10-05" version="1">MSLRLTISCFENFSSLILYLSFSCLFLNLVSFFILSLPSSCLFLHPISYFILLPLSYLASFFLPPPLYLFPHLISCLFSASPGCLMYLQRSYRCLLASHSAALALFFFTLFTSPRQTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGM0</accession>
    <name>A0A1A0HGM0_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DUF962-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_76211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713621.1">
        <property type="nucleotide sequence ID" value="XM_018858494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGM0"/>
    <dbReference type="GeneID" id="30031470"/>
    <dbReference type="OrthoDB" id="2124888at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046521">
        <property type="term" value="P:sphingoid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026:SF9">
        <property type="entry name" value="2-HYDROXY-PALMITIC ACID DIOXYGENASE MPO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="Mpo1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="186" mass="21351" checksum="6E93BBBAFD400FB8" modified="2016-10-05" version="1">MPGIFDIKAHLVFYRKYHFNNTNVAIHLVCIPIILLSAISFSIGNVIFTQYPYITTGVVAAWLYGLYYVILDWQLGVPAFLFLAGFAHVQRVYYLELSPSSLILRSLYKQIAITAHIISWLAQFYGHAVYERRAPALFDNLLQAVVLAPFFVVYEIAFWLGFKLDIKKEMDNQAGKFVSEMSKKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGQ1</accession>
    <name>A0A1A0HGQ1_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase DBP2</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP-dependent RNA helicase dbp2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_49334</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713514.1">
        <property type="nucleotide sequence ID" value="XM_018858281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGQ1"/>
    <dbReference type="STRING" id="869754.A0A1A0HGQ1"/>
    <dbReference type="GeneID" id="30031257"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002151">
        <property type="term" value="F:G-quadruplex RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071042">
        <property type="term" value="P:nuclear polyadenylation-dependent mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13 18" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="4 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="140"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="330"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="482"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="12">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="14">
        <location>
            <begin position="513"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA23033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="539" mass="59316" checksum="6BFCC3FAE70FD69D" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-04-02" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGT6</accession>
    <name>A0A1A0HGT6_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">METBIDRAFT_29730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713686.1">
        <property type="nucleotide sequence ID" value="XM_018855468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGT6"/>
    <dbReference type="STRING" id="869754.A0A1A0HGT6"/>
    <dbReference type="GeneID" id="30028444"/>
    <dbReference type="OrthoDB" id="2320368at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_B">
        <property type="entry name" value="Ribosomal_uS2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005706">
        <property type="entry name" value="Ribosomal_uS2_bac/mit/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01011">
        <property type="entry name" value="rpsB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S2 PROKARYOTIC AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12534:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="141"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="458"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="469"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="OBA23205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="499" mass="54829" checksum="B04C03653CF53B7D" modified="2016-10-05" version="1">MLRISGRARSTMCQRRLVGGMVRMNSTATVGTRDTATEPALAEVAQQKLREKVMAEALAKEEQAMLKAKALRELKERTRLAVHALNNTPSQALEDRMLHLQSQLDLLPDQNNVKQLDEQIEDFLFESMRLPAAEVGYAPWTRSSENHKDGTGQVGSAPAQRIRTTSSGSIAHQFPNLQPTPDYKAYSEQELFLRHMAHVGHTGHLGTHQTQVYEPSEDTFNPRSVSELSIAQLMAAECHLGHAKALWRPSTQPFIYGEYQGVHLIDLNHTLVALRKAAKVTRAVAAKGGVILYVGTSRLKEQQVALEEAARRSNAYCVTHRWIPGTITNFTEVSRQILGPQKALVNLADEPTGKYFTTTEGTIIKPDLIVLMNPVENRNCIGESIKLRIPTIGLCDTNMEPTLLTYPVPCNDDSMRASSLILGVLLRAAQEGVQERKLAFAHYKQAQGASLAATKMEMEKKTGDAATESVATESVATESVATDVATSDRAPTAETEQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGU6</accession>
    <name>A0A1A0HGU6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">METBIDRAFT_98091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713554.1">
        <property type="nucleotide sequence ID" value="XM_018859463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGU6"/>
    <dbReference type="GeneID" id="30032438"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="OBA23073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="89" mass="9913" checksum="3837E45B99E0FF6D" modified="2016-10-05" version="1">MIIIRHVIVIFIKVVFILVISRYLIIRPRRGSHNHIHCIASHRTCSPISSLCTLLLSSIAIQLSFALSPLSFALSRNSCLQSTLLLFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HGX8</accession>
    <name>A0A1A0HGX8_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GDPD-domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">METBIDRAFT_29771</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713732.1">
        <property type="nucleotide sequence ID" value="XM_018855477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HGX8"/>
    <dbReference type="STRING" id="869754.A0A1A0HGX8"/>
    <dbReference type="GeneID" id="30028453"/>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047389">
        <property type="term" value="F:glycerophosphocholine phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd14484">
        <property type="entry name" value="SPX_GDE1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="6">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="518"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="7">
        <location>
            <begin position="917"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="684"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="699"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="OBA23251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1277" mass="142843" checksum="FD9A578D11A3DBC2" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH46</accession>
    <name>A0A1A0HH46_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein DOM34 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">METBIDRAFT_38160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the Dom34-Hbs1 complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway (PubMed:20890290). In the Dom34-Hbs1 complex, dom34 recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following ribosome-binding, the Dom34-Hbs1 complex promotes the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713802.1">
        <property type="nucleotide sequence ID" value="XM_018856964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH46"/>
    <dbReference type="STRING" id="869754.A0A1A0HH46"/>
    <dbReference type="GeneID" id="30029940"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990533">
        <property type="term" value="C:Dom34-Hbs1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000001">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000008">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0469">Meiosis</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="OBA23321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="387" mass="43077" checksum="7A2ACB237991F1D7" modified="2016-10-05" version="1">MKLLDKTIALEKDKSGLLSLVPQDKEDLWQLYNLIQKGDVVELLSIRNVKKTEGGKAERVHVRLKLAVESVDYAPSDEAMRIRGKTTEQSEYVPNQSYHTAEVQLNKSLKINKPEWDDISYGIVLEASSVEKKAEVGAVVLQEGVAHLCLVTDKMTVLRNKIEKAIPRKSRGDAGGSAHDKAMAKFLDMVQLTLSRNFDIEKLKVIILASPGFTAQALYKALLDTAIKEDNKTILKNKSKFLVVHSSTGYLQGLEEVLKDPSVQKNLNNTKFAKEAAVFEEFQKVLNEDDGRAWYGPKEVVRAVESGAVKSLLLTDALFRSDDIAVRKHYISLSEDVKNQGGDVYVLSTLHDCGVQLDQLTGAAVLLKYPVDLESDDDDEDEDDDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HH53</accession>
    <name>A0A1A0HH53_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">METBIDRAFT_76512</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713990.1">
        <property type="nucleotide sequence ID" value="XM_018858542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HH53"/>
    <dbReference type="STRING" id="869754.A0A1A0HH53"/>
    <dbReference type="GeneID" id="30031518"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="633"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="OBA23509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="1002" mass="113060" checksum="EF7C8D36D22CF7FD" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHC6</accession>
    <name>A0A1A0HHC6_9ASCO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Beta-sandwich domain of Sec23/24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">METBIDRAFT_29762</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="LXTC01000001">
        <property type="protein sequence ID" value="OBA23243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_018713724.1">
        <property type="nucleotide sequence ID" value="XM_018855475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A1A0HHC6"/>
    <dbReference type="STRING" id="869754.A0A1A0HHC6"/>
    <dbReference type="GeneID" id="30028451"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000092555">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803:SF4">
        <property type="entry name" value="SECRETORY 24CD, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="7">
        <location>
            <begin position="244"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="8">
        <location>
            <begin position="325"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="10">
        <location>
            <begin position="578"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="9">
        <location>
            <begin position="683"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="OBA23243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000092555"/>
        </source>
    </evidence>
    <sequence length="989" mass="108173" checksum="6BF36AFD67FCEC8B" modified="2016-10-05" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2016-10-05" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>A0A1A0HHR5</accession>
    <name>A0A1A0HHR5_9ASCO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Purine-cytosine permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">METBIDRAFT_29996</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993</name>
        <dbReference type="NCBI Taxonomy" id="869754"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Ascomycota</taxon>
            <taxon>Saccharomycotina</taxon>
            <taxon>Pichiomycetes</taxon>
            <taxon>Metschnikowiaceae</taxon>
            <taxon>Metschnikowia</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="submission" date="2016-05" db="EMBL/GenBank/DDBJ databases">
            <title>Comparative genomics of biotechnologically important yeasts.</title>
            <authorList>
                <consortium name="DOE Joint Genome Institute"/>
                <person name="Riley R."/>
                <person name="Haridas S."/>
                <person name="Wolfe K.H."/>
                <person name="Lopes M.R."/>
                <person name="Hittinger C.T."/>
                <person name="Goker M."/>
                <person name="Salamov A."/>
                <person name="Wisecaver J."/>
                <person name="Long T.M."/>
                <person name="Aerts A.L."/>
                <person name="Barry K."/>
                <person name="Choi C."/>
                <person name="Clum A."/>
                <person name="Coughlan A.Y."/>
                <person name="Deshpande S."/>
                <person name="Douglass A.P."/>
                <person name="Hanson S.J."/>
                <person name="Klenk H.-P."/>
                <person name="LaButti K."/>
                <person name="Lapidus A."/>
                <person name="Lindquist E."/>
                <person name="Lipzen A."/>
                <person name="Meier-kolthoff J.P."/>
                <person name="Ohm R.A."/>
                <person name="Otillar R.P."/>
                <person name="Pangilinan J."/>
                <person name="Peng Y."/>
                <person name="Rokas A."/>
                <person name="Rosa C.A."/>
                <person name="Scheuner C."/>
                <person name="Sibirny A.A."/>
                <person name="Slot J.C."/>
                <person name="Stielow J.B."/>
                <person name="Sun H."/>
                <person name="Kurtzman C.P."/>
                <person name="Blackwell M."/>
                <person name="Grigoriev I.V."/>
                <person name="Jeffries T.W."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">NRRL YB-4993</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane pr